Rh6AG050600

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000644
Physical Location & Seq
Reverse (-)
7265995 .. 7278182
12188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG050600.1

Sequence Viewer

Length: 1710 bp
ATGTCAAATTACAATAGGGGGAAGACACCTTGCGGGCACAACTTCTGCCTCAAATGCTTTGAGAAATGGGTGGCGCAGGGTAAACTTACATGTGCAAATTGCCGCCAATCGATCCCCTCCACTCTGAGAATCAACTCTAATCTTGTGGGAAGCATTCGTATTGCCAGGGCATCACAACGCAGCGGCTCAGGGGAGTACCTACCTCAGTTGGCTCATCGCATTCGAAACCAGGACCGTCCAGACAAAGCATTTACAACCGAGCGAGCCAAGAAGCCTGGGAAAGCTAATGCCTCAAGTGGAAGGATCTTTGTGACATCAGCTCCGGATCATTTTGGGCCCATAACAGCCGAATATGATCCTGAGAAGAATAAGGGTGTGGCGGTGGGGGAGACTTGGGGGCATAGGATTGAATGTAGACAATGGGGAATTCATCGCCCTATGGTTTCCGGGATTTCTGGGCAGTGCAAGTATGGCGCCCAATCAGTGGTTCTCTCAGGAGGGTATATAGACGATGAAGATCATGGTGAATGGTTCATTTACACCGGGAGTTGTGGCAAGGATTTGAGTCGAAACAAGCGTACCAACAAGCAACACAGTAAAGATCAGATGTTCACAAAGCTTAATCAGTCACTCAGGCTAAGTTGCCTAAATGGTTATCCTGTTCGAGTAGTGAGATCGCACAAGGAGACACGCTCTTCGTATGCGCCTACGGAGGTGGGACTGCGATATGATGGGGTTTACAGGATTGAAAAATGTTGGCGTAAAGATGGAACACAGGGTTATAAGGTTTGCAGATATCTTTTTGTAAGATGTGACAACGAACCTGCGCCTTGGACGAGGTATGTTATGATGAAACACTCACTCTCTCTCTCTGCACCTTTAATTCTTGGAAAATTTATCTGCAGTGATGATCATGGGGACCGTCCAAGGCCTCTGCCAGATATTGCAGAGTTGAACAGTGCCATTGATATAACAAATACAAAGGGTTCTCCTTCTTGGGACAATGATGCAGAAAAAGCGTGCTGGATGTGGAAAAAGCCTCGACCACTTAGCAGACAACTGGTGGATAGGGGAGAACCAGCTGGGAAGAAAATTAGGAGAGTTAGACGGCAGGAACAGAATGCGAAAGAAAAAGTTCTCAAAGGGTTTAGGTGTGATAAATGTCAGAAGATTATGACTGAACCAGTTACTACACCATGTGGTCATAACTTCTGCAAAGCTTGTCTGCAAGATACCTTTGCTGGTATAAGTTTAATAAAACAGAGGACGTGCCAAGGCGGAAGAACATTGCGAGCACAAAAGAACGTCATGAAATGCCCAAAGTGTCCACATGACATAGCTGACTTTCTTCAAAATCCACAGGTTAATAAGAATCTAGAGAACGCCATAGAATTACTCCATCAGAAAGAGGAAGATGTTGCAGAGGAAGTGGAAGACGCTGAGGAAGATGAAGATGATGAAGAAGAGGAAGAAGAGGAAGAAGCTGAAGAAACTTACAATGCCGGTGAAGAGAGTGAAGCCGAAGACAAAGGAAGCATCCCACCAATACCTCAGACTGAAACTACAGAGCAACAAACTAGTGAGCAAAAGAAACCAAGTCATAAGCGAAATAGGTCGAGCAATGGAGACAACGTGTTGGATGGCGGAGCACTGACCAAGAGAAGCAAGAGGAGTAAAGTGGGAGCAGCTAGTAGTCTGATTGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

569

Amino Acids

64.1

Weight (kDa)

8.69

Isoelectric Point (pI)

53.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 123 - 273 5.6e-47 SAD/SRA domain
zf-RING_UBOX PF13445 385 - 416 5.3e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 783
Acc36I ACCTGC 1 cut(s) 832
AccB1I GGYRCC 1 cut(s) 473
AccB7I CCANNNNNTGG 1 cut(s) 484
AccI GTMKAC 1 cut(s) 415
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 6 cut(s) 33, 103, 183, 380, 1278, 1644
AclWI GGATC 4 cut(s) 106, 311, 333, 350
AcsI RAATTY 2 cut(s) 426, 893
AcuI CTGAAG 1 cut(s) 1506
AcyI GRCGYC 1 cut(s) 474
AdeI CACNNNGTG 1 cut(s) 1199
AfaI GTAC 2 cut(s) 197, 580
AfiI CCNNNNNNNGG 1 cut(s) 484
AflIII ACRYGT 2 cut(s) 89, 1632
AgsI TTSAA 4 cut(s) 410, 749, 955, 1352
AhlI ACTAGT 1 cut(s) 1577
AjiI CACGTC 1 cut(s) 1269
AjnI CCWGG 3 cut(s) 164, 228, 274
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 8 cut(s) 284, 320, 619, 1082, 1220, 1340, 1484, 1688
AluI AGCT 8 cut(s) 284, 320, 619, 1082, 1220, 1340, 1484, 1688
Alw21I GWGCWC 2 cut(s) 1297, 1651
Alw26I GTCTC 3 cut(s) 383, 680, 1620
AlwI GGATC 4 cut(s) 106, 311, 333, 350
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 2 cut(s) 335, 929
ApaI GGGCCC 1 cut(s) 339
ApeKI GCWGC 2 cut(s) 180, 1685
ApoI RAATTY 2 cut(s) 426, 893
ArsI GACNNNNNNTTYG 2 cut(s) 679, 711
Asp700I GAANNNNTTC 1 cut(s) 1134
AspLEI GCGC 4 cut(s) 76, 476, 706, 829
AspS9I GGNCC 4 cut(s) 232, 335, 336, 919
AsuC2I CCSGG 2 cut(s) 448, 544
AsuHPI GGTGA 2 cut(s) 536, 1517
AsuII TTCGAA 1 cut(s) 223
AvaII GGWCC 2 cut(s) 232, 919
BaeGI GKGCMC 2 cut(s) 39, 339
BaeI ACNNNNGTAYC 2 cut(s) 562, 595
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 339
BbsI GAAGAC 3 cut(s) 29, 1440, 1530
Bbv12I GWGCWC 2 cut(s) 1297, 1651
BbvCI CCTCAGC 1 cut(s) 1440
BbvI GCAGC 2 cut(s) 192, 1697
BccI CCATC 4 cut(s) 725, 761, 1407, 1634
BceAI ACGGC 1 cut(s) 1124
BciT130I CCWGG 3 cut(s) 166, 230, 276
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 2 cut(s) 448, 544
BcoDI GTCTC 3 cut(s) 383, 680, 1620
BcuI ACTAGT 1 cut(s) 1577
BfaI CTAG 3 cut(s) 1376, 1578, 1689
BfmI CTRYAG 2 cut(s) 901, 1563
BfoI RGCGCY 1 cut(s) 477
BfuAI ACCTGC 1 cut(s) 832
BisI GCNGC 4 cut(s) 103, 181, 184, 1686
BlsI GCNGC 4 cut(s) 104, 182, 185, 1687
Bme1390I CCNGG 5 cut(s) 166, 230, 276, 448, 544
Bme18I GGWCC 2 cut(s) 232, 919
BmgBI CACGTC 1 cut(s) 1269
BmgT120I GGNCC 4 cut(s) 232, 335, 336, 919
BmiI GGNNCC 3 cut(s) 337, 475, 920
BmrFI CCNGG 5 cut(s) 166, 230, 276, 448, 544
BmsI GCATC 3 cut(s) 179, 997, 1545
BpiI GAAGAC 3 cut(s) 29, 1440, 1530
BplI GAGNNNNNCTC 2 cut(s) 677, 709
Bpu10I CCTNAGC 2 cut(s) 187, 1440
Bpu14I TTCGAA 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 277
BpuMI CCSGG 2 cut(s) 448, 544
Bsa29I ATCGAT 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 5 cut(s) 165, 275, 830, 926, 1273
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 4 cut(s) 304, 334, 1617, 1647
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse118I RCCGGY 1 cut(s) 1502
Bse1I ACTGG 2 cut(s) 1065, 1184
Bse3DI GCAATG 2 cut(s) 1286, 1627
Bse8I GATNNNNATC 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 322
BseBI CCWGG 3 cut(s) 166, 230, 276
BseCI ATCGAT 1 cut(s) 110
BseDI CCNNGG 5 cut(s) 165, 275, 830, 926, 1273
BseGI GGATG 3 cut(s) 1032, 1536, 1645
BseJI GATNNNNATC 1 cut(s) 516
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMI GCAATG 2 cut(s) 1286, 1627
BseMII CTCAG 8 cut(s) 116, 201, 218, 351, 507, 646, 1431, 1565
BseNI ACTGG 2 cut(s) 1065, 1184
BseRI GAGGAG 1 cut(s) 1684
BseSI GKGCMC 2 cut(s) 39, 339
BseXI GCAGC 2 cut(s) 192, 1697
BseYI CCCAGC 1 cut(s) 1082
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 2 cut(s) 337, 931
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 110
BsiHKAI GWGCWC 2 cut(s) 1297, 1651
BsiSI CCGG 4 cut(s) 323, 447, 543, 1503
BslFI GGGAC 3 cut(s) 732, 932, 1013
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 3 cut(s) 383, 680, 1620
BsmFI GGGAC 3 cut(s) 732, 932, 1013
BsmI GAATGC 3 cut(s) 153, 219, 1126
BsnI GGCC 2 cut(s) 337, 931
Bsp119I TTCGAA 1 cut(s) 223
Bsp120I GGGCCC 1 cut(s) 335
Bsp1286I GDGCHC 4 cut(s) 39, 339, 1297, 1651
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 8 cut(s) 111, 303, 325, 355, 517, 601, 674, 910
BspACI CCGC 6 cut(s) 33, 103, 183, 380, 1278, 1644
BspANI GGCC 2 cut(s) 337, 931
BspCNI CTCAG 8 cut(s) 117, 200, 217, 352, 506, 645, 1432, 1564
BspDI ATCGAT 1 cut(s) 110
BspEI TCCGGA 1 cut(s) 322
BspHI TCATGA 2 cut(s) 1308, 1706
BspLI GGNNCC 3 cut(s) 337, 475, 920
BspMAI CTGCAG 1 cut(s) 905
BspMI ACCTGC 1 cut(s) 832
BspPI GGATC 4 cut(s) 106, 311, 333, 350
BspQI GCTCTTC 1 cut(s) 700
BspT104I TTCGAA 1 cut(s) 223
BspT107I GGYRCC 1 cut(s) 473
BsrDI GCAATG 2 cut(s) 1286, 1627
BsrFI RCCGGY 1 cut(s) 1502
BsrI ACTGG 2 cut(s) 1065, 1184
BssAI RCCGGY 1 cut(s) 1502
BssECI CCNNGG 5 cut(s) 165, 275, 830, 926, 1273
BssMI GATC 8 cut(s) 111, 303, 325, 355, 517, 601, 674, 910
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 3 cut(s) 830, 926, 1273
Bst2UI CCWGG 3 cut(s) 166, 230, 276
Bst4CI ACNGT 4 cut(s) 236, 596, 923, 959
Bst6I CTCTTC 4 cut(s) 700, 1458, 1467, 1503
BstACI GRCGYC 1 cut(s) 474
BstBI TTCGAA 1 cut(s) 223
BstC8I GCNNGC 4 cut(s) 35, 264, 1021, 1293
BstF5I GGATG 3 cut(s) 1032, 1536, 1645
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 4 cut(s) 76, 476, 706, 829
BstKTI GATC 8 cut(s) 114, 306, 328, 358, 520, 604, 677, 913
BstMAI GTCTC 3 cut(s) 383, 680, 1620
BstMBI GATC 8 cut(s) 111, 303, 325, 355, 517, 601, 674, 910
BstMWI GCNNNNNNNGC 3 cut(s) 54, 471, 1016
BstNI CCWGG 3 cut(s) 166, 230, 276
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 5 cut(s) 164, 228, 274, 446, 542
BstSFI CTRYAG 2 cut(s) 901, 1563
BstSLI GKGCMC 2 cut(s) 39, 339
BstV1I GCAGC 2 cut(s) 192, 1697
BstV2I GAAGAC 3 cut(s) 29, 1440, 1530
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
Bsu15I ATCGAT 1 cut(s) 110
BsuRI GGCC 2 cut(s) 337, 931
BsuTUI ATCGAT 1 cut(s) 110
BtgZI GCGATG 2 cut(s) 200, 416
BtrI CACGTC 1 cut(s) 1269
BtsCI GGATG 3 cut(s) 1032, 1536, 1645
BtsI GCAGTG 2 cut(s) 467, 910
BtsIMutI CAGTG 5 cut(s) 467, 489, 910, 964, 1649
BveI ACCTGC 1 cut(s) 832
Cac8I GCNNGC 4 cut(s) 35, 264, 1021, 1293
CciI TCATGA 2 cut(s) 1308, 1706
CfoI GCGC 4 cut(s) 76, 476, 706, 829
Cfr10I RCCGGY 1 cut(s) 1502
Cfr13I GGNCC 4 cut(s) 232, 335, 336, 919
ClaI ATCGAT 1 cut(s) 110
CseI GACGC 1 cut(s) 1445
Csp6I GTAC 2 cut(s) 196, 579
CviAII CATG 7 cut(s) 90, 521, 914, 1197, 1309, 1331, 1707
CviQI GTAC 2 cut(s) 196, 579
DinI GGCGCC 1 cut(s) 475
DpnI GATC 8 cut(s) 113, 305, 327, 357, 519, 603, 676, 912
DpnII GATC 8 cut(s) 111, 303, 325, 355, 517, 601, 674, 910
DraIII CACNNNGTG 1 cut(s) 1199
Eam1104I CTCTTC 4 cut(s) 700, 1458, 1467, 1503
EarI CTCTTC 4 cut(s) 700, 1458, 1467, 1503
EciI GGCGGA 2 cut(s) 1293, 1659
Eco130I CCWWGG 3 cut(s) 830, 926, 1273
Eco147I AGGCCT 1 cut(s) 931
Eco24I GRGCYC 1 cut(s) 339
Eco32I GATATC 1 cut(s) 797
Eco47I GGWCC 2 cut(s) 232, 919
Eco57I CTGAAG 1 cut(s) 1506
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 3 cut(s) 164, 228, 274
EcoRV GATATC 1 cut(s) 797
EcoT14I CCWWGG 3 cut(s) 830, 926, 1273
EcoT38I GRGCYC 1 cut(s) 339
EgeI GGCGCC 1 cut(s) 475
EheI GGCGCC 1 cut(s) 475
ErhI CCWWGG 3 cut(s) 830, 926, 1273
FaeI CATG 7 cut(s) 93, 524, 917, 1200, 1312, 1334, 1710
FaqI GGGAC 3 cut(s) 732, 932, 1013
FatI CATG 7 cut(s) 89, 520, 913, 1196, 1308, 1330, 1706
FauI CCCGC 1 cut(s) 26
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 1 cut(s) 415
Fnu4HI GCNGC 4 cut(s) 103, 181, 184, 1686
FokI GGATG 3 cut(s) 1039, 1523, 1652
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 103, 181, 184, 1686
FspBI CTAG 3 cut(s) 1376, 1578, 1689
GlaI GCGC 4 cut(s) 75, 475, 705, 828
GluI GCNGC 4 cut(s) 103, 181, 184, 1686
GsaI CCCAGC 1 cut(s) 1086
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 2 cut(s) 337, 931
HapII CCGG 4 cut(s) 323, 447, 543, 1503
HgaI GACGC 1 cut(s) 1445
HhaI GCGC 4 cut(s) 76, 476, 706, 829
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 7 cut(s) 93, 524, 917, 1200, 1312, 1334, 1710
Hin6I GCGC 4 cut(s) 74, 474, 704, 827
HinP1I GCGC 4 cut(s) 74, 474, 704, 827
HindIII AAGCTT 2 cut(s) 617, 1218
HinfI GANTC 4 cut(s) 129, 565, 1372, 1703
HpaII CCGG 4 cut(s) 323, 447, 543, 1503
HphI GGTGA 2 cut(s) 536, 1517
Hpy166II GTNNAC 5 cut(s) 83, 416, 612, 739, 1328
Hpy188I TCNGA 6 cut(s) 126, 606, 1167, 1404, 1554, 1698
Hpy188III TCNNGA 7 cut(s) 239, 323, 359, 495, 1309, 1376, 1707
Hpy8I GTNNAC 5 cut(s) 83, 416, 612, 739, 1328
HpyAV CCTTC 2 cut(s) 294, 1002
HpyCH4III ACNGT 4 cut(s) 236, 596, 923, 959
HpyCH4IV ACGT 3 cut(s) 1268, 1305, 1632
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 471, 1016
HpySE526I ACGT 3 cut(s) 1268, 1305, 1632
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 7 cut(s) 93, 524, 917, 1200, 1312, 1334, 1710
HspAI GCGC 4 cut(s) 74, 474, 704, 827
KasI GGCGCC 1 cut(s) 473
Kpn2I TCCGGA 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 8 cut(s) 111, 303, 325, 355, 517, 601, 674, 910
LguI GCTCTTC 1 cut(s) 700
LmnI GCTCC 3 cut(s) 325, 1646, 1682
Lsp1109I GCAGC 2 cut(s) 192, 1697
LweI GCATC 3 cut(s) 179, 997, 1545
MaeI CTAG 3 cut(s) 1376, 1578, 1689
MaeII ACGT 3 cut(s) 1268, 1305, 1632
MaeIII GTNAC 4 cut(s) 310, 627, 812, 1186
MalI GATC 8 cut(s) 113, 305, 327, 357, 519, 603, 676, 912
MboI GATC 8 cut(s) 111, 303, 325, 355, 517, 601, 674, 910
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 4 cut(s) 39, 339, 1297, 1651
MluCI AATT 7 cut(s) 7, 97, 426, 882, 893, 1092, 1391
Mly113I GGCGCC 1 cut(s) 474
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 1 cut(s) 1617
MroI TCCGGA 1 cut(s) 322
MroXI GAANNNNTTC 1 cut(s) 1134
MseI TTAA 4 cut(s) 621, 881, 1253, 1365
MspA1I CMGCKG 2 cut(s) 183, 1082
MspI CCGG 4 cut(s) 323, 447, 543, 1503
MspR9I CCNGG 5 cut(s) 166, 230, 276, 448, 544
Mva1269I GAATGC 3 cut(s) 153, 219, 1126
MvaI CCWGG 3 cut(s) 166, 230, 276
MwoI GCNNNNNNNGC 3 cut(s) 54, 471, 1016
NarI GGCGCC 1 cut(s) 474
NciI CCSGG 2 cut(s) 448, 544
NdeII GATC 8 cut(s) 111, 303, 325, 355, 517, 601, 674, 910
NlaIII CATG 7 cut(s) 93, 524, 917, 1200, 1312, 1334, 1710
NlaIV GGNNCC 3 cut(s) 337, 475, 920
NmuCI GTSAC 3 cut(s) 310, 627, 812
NspI RCATGY 1 cut(s) 93
NspV TTCGAA 1 cut(s) 223
PagI TCATGA 2 cut(s) 1308, 1706
PceI AGGCCT 1 cut(s) 931
PciI ACATGT 1 cut(s) 89
PciSI GCTCTTC 1 cut(s) 700
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PctI GAATGC 3 cut(s) 153, 219, 1126
PdmI GAANNNNTTC 1 cut(s) 1134
PfeI GAWTC 3 cut(s) 129, 1372, 1703
PflMI CCANNNNNTGG 1 cut(s) 484
PfoI TCCNGGA 1 cut(s) 446
PkrI GCNGC 4 cut(s) 104, 182, 185, 1687
PleI GAGTC 1 cut(s) 573
PluTI GGCGCC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 573
PscI ACATGT 1 cut(s) 89
PsiI TTATAA 1 cut(s) 783
Psp6I CCWGG 3 cut(s) 164, 228, 274
PspFI CCCAGC 1 cut(s) 1082
PspGI CCWGG 3 cut(s) 164, 228, 274
PspN4I GGNNCC 3 cut(s) 337, 475, 920
PspOMI GGGCCC 1 cut(s) 335
PspPI GGNCC 4 cut(s) 232, 335, 336, 919
PstI CTGCAG 1 cut(s) 905
PsuI RGATCY 1 cut(s) 303
PvuII CAGCTG 1 cut(s) 1082
RsaI GTAC 2 cut(s) 197, 580
RsaNI GTAC 2 cut(s) 196, 579
SapI GCTCTTC 1 cut(s) 700
SaqAI TTAA 4 cut(s) 621, 881, 1253, 1365
SatI GCNGC 4 cut(s) 103, 181, 184, 1686
Sau3AI GATC 8 cut(s) 111, 303, 325, 355, 517, 601, 674, 910
Sau96I GGNCC 4 cut(s) 232, 335, 336, 919
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 5 cut(s) 166, 230, 276, 448, 544
SduI GDGCHC 4 cut(s) 39, 339, 1297, 1651
SfaNI GCATC 3 cut(s) 179, 997, 1545
SfcI CTRYAG 2 cut(s) 901, 1563
SfoI GGCGCC 1 cut(s) 475
SfuI TTCGAA 1 cut(s) 223
SinI GGWCC 2 cut(s) 232, 919
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
SpeI ACTAGT 1 cut(s) 1577
Sse9I AATT 7 cut(s) 7, 97, 426, 882, 893, 1092, 1391
SseBI AGGCCT 1 cut(s) 931
SsiI CCGC 6 cut(s) 33, 103, 183, 380, 1278, 1644
SspDI GGCGCC 1 cut(s) 473
SspMI CTAG 3 cut(s) 1376, 1578, 1689
StuI AGGCCT 1 cut(s) 931
StyD4I CCNGG 5 cut(s) 164, 228, 274, 446, 542
StyI CCWWGG 3 cut(s) 830, 926, 1273
TaaI ACNGT 4 cut(s) 236, 596, 923, 959
TaiI ACGT 3 cut(s) 1271, 1308, 1635
TaqI TCGA 6 cut(s) 110, 223, 568, 664, 1042, 1616
TasI AATT 7 cut(s) 7, 97, 426, 882, 893, 1092, 1391
TauI GCSGC 2 cut(s) 105, 186
TfiI GAWTC 3 cut(s) 129, 1372, 1703
Tru1I TTAA 4 cut(s) 621, 881, 1253, 1365
Tru9I TTAA 4 cut(s) 621, 881, 1253, 1365
TscAI CASTG 5 cut(s) 467, 489, 910, 964, 1656
TseFI GTSAC 3 cut(s) 310, 627, 812
TseI GCWGC 2 cut(s) 180, 1685
Tsp45I GTSAC 3 cut(s) 310, 627, 812
TspDTI ATGAA 8 cut(s) 419, 523, 528, 866, 1325, 1464, 1473, 1695
TspGWI ACGGA 1 cut(s) 725
TspRI CASTG 5 cut(s) 467, 489, 910, 964, 1656
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 232, 919
XapI RAATTY 2 cut(s) 426, 893
XbaI TCTAGA 1 cut(s) 1375
XceI RCATGY 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 415
XmnI GAANNNNTTC 1 cut(s) 1134
XspI CTAG 3 cut(s) 1376, 1578, 1689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.