FvH4_5g09600

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000644
Physical Location & Seq
Reverse (-)
5545578 .. 5550668
5091 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09600.t2

Sequence Viewer

Length: 2412 bp
ATGGCGCACGTTATCCACCTCCCGTGCGACGGAGACGGCGTCTGCATGCTCTGCAAAACGACGCCGTCGGACGAGGACCGGCTCACCTGCAAGACCTGCGCCACGCCGTGGCACGTGAGCTGTCTCTCGTCCCGTCCCGAATCGCTCGCTTCCACTCTCCACTGGGAGTGCCCGGACTGTTCTTCACTCACCGGCGACACGCCGGCCGTGCAATCGGCAGTCTCCGGCGACCTGATAGCCGCGATCCGGGCGATCGAGGCCGATGCGGCGCTGACGGAGCAGCAGAAGGCGTGTAAGCGACAGGAGCTGATGAGCGGGAAGGCGCAGCAGGAGGAAGACGAAGGCTGCGCCGGCAGGAAGGGAGTAAACGACGGCGTTTTGGATGGGATTTTCAATTGCTCGTTCTGTATGCAACTACCGGAGCGACCTGTGACGACACCATGTGGCCACAATTTCTGCTTGAAGTGCTTCCAGAAATGGGTTGGACAAGGAAAGCGTACTTGTGCAAAGTGTCGCCATGTCATCCCTTCCAAAATGGCAAGTCAACCCCGCATCAATTCTTCACTTGTAGTTGCCATACGTATGGCGAAGCTGTCAAAATCACATGTTACTGAGGGGCCTGCAAAGTTATACCACTTTGTTCGAAACCAAGACAGGCCTGACAAAGCTTTCACTACTGAGCGGGCGCAAAATTCAGGAAAATCTAATGCTGCAAGTGGCAAGATATTTGTCACAATTCCAGCTGATCATTTTGGTCCTATAACTGCTGAATATGATCCTGAGAGAAACCAAGGGGTGCTGGTTGGGGAATGCTGGGAGGATAGGCTTGAATGTCGACAGTGGGGTGCACACTTTCCCCATGTTGCTGGTATAGCTGGACAGTCTAACCATGGTGCACAGTCAGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATACACAGGAAGTGGTGGAAGGGATTTGAGTGGGAATAAGCGAACAAACAAGGATCAGTCATTTGACCAGACATTTGAGAAATATAACAAGGCTCTCCAAGTTAGTTGCATGAAAGGGTATCCTTTACGGGTGGTAAGATCACACAAGGAGAAGCGCTCCTCATATGCCCCTGAGAAAGGGTTGCGGTATGATGGAATCTATAGAATTGAAAAGTGTTGGACAAAGGTGGGAATCCAGGGGTTCAAGGTTTGCCGGTATCTTTTTGTTAGATGTGACAATGAACCTGCACCATGGACGAGTGATCAACACGGAGACCGGCCTAGACCTTTACCTGCAATACCTGAGCTGAAGAAAGCTACAAGTATAATAGAGAGGAGAACAAGTCCATCATGGGACTTTGAGGAAAAAGATGGCTGCTGGAAGTGGAAAAAATCACCACCTATCAGTAAAAAAACTGTTCAGACGTTAGATCCTGATGATCGAAAGAGGTCAAGAAGAGGAAGGAGGAACGCAAGGGATATTACTGTCCGAGAGAGGCTCTTAAGAGAATTCAGCTGCCTTCTTTGCGGGGAGGTCATGACATTACCAATAACCACTCCTTGTGCGCATAATTTTTGCAAGTCGTGTTTAGAAGGCGCTTTTACGGGAAAGAGTTTGGTAAGAGAAAGAAACAGAGGGGGAAGAACCCTCCGAACCCAAAAGAATATAATGAGTTGCCCTTCTTGCCCCACTGATATCTCTGATTTTCTTCAAAATCCTCAGGTGAACAGAGAGTTAATGGATGCGATTGTGTCACTTAAGTCACAGAAAGAAGAGGAAGAGAATGGGGATTCAGCTGAAGAAATGAGCCAAGATGTGGATAATTCTGACAACGAAATCACTGAAATTGTTGAAGATGCAGGTGTTAGAGAATACGCAGAGAATTCTCCTACAAAGTGCAACTCCGAAAGGAGTCCCAAACGACAGAAAGTTGATGCTGTGTTAGGATCACCAAAAACTGATGATGAGGATGTAAATGAAATTCACATCACCCGGACTAATGGCAGAGGCTCACCGGAAACCAATGATGAGGAGGTAAATGGAATTCATGTCACCCATGCTAATGGCAAAGGCTCACCGGAAACCAATGTTGAGGAGGTAAATGGAATTCATGTCACCCATGCTAATGGCAAAGGCTCACCGGAAACCAATGTTGAGGAGGTAAATGGAATTCATGTCACCCATGCTAATGGCAAAGGCTCACCGGAAACCAATGTTGAGGAGGTAAATGAAATTCCTGTCACCCATACAAATGGAACAGGCTCACCGGTTCCAGTTGCTAATTGCGGCAAGCAAGATGCTAGTCCAGGTGGTGTTAAAGCAATTACTAGAGAAAAGATGAAGGCCGAGGAAGTAGTAGGAGAAGATAATGGTTCCCCATCAAGTCCTCTCCTTGTGCAATCTGATTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

804

Amino Acids

88.58

Weight (kDa)

6.22

Isoelectric Point (pI)

56.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 132 - 172 1.6e-06 Ring finger domain
zf-C3HC4_3 PF13920 132 - 175 1.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 133 - 171 3.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 133 - 171 3.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 133 - 171 5.3e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 263 - 412 5e-49 SAD/SRA domain
zf-RING_UBOX PF13445 505 - 536 9.7e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 95, 1847
Acc16I TGCGCA 1 cut(s) 1563
Acc36I ACCTGC 5 cut(s) 95, 104, 1249, 1297, 1847
Acc65I GGTACC 1 cut(s) 921
AccB1I GGYRCC 1 cut(s) 921
AccB7I CCANNNNNTGG 2 cut(s) 108, 1813
AccBSI CCGCTC 2 cut(s) 315, 682
AccI GTMKAC 1 cut(s) 835
AccII CGCG 1 cut(s) 242
AciI CCGC 8 cut(s) 240, 266, 315, 550, 682, 1141, 1524, 2283
AclWI GGATC 6 cut(s) 238, 770, 945, 1017, 1421, 1951
AcoI YGGCCR 2 cut(s) 204, 445
AcsI RAATTY 8 cut(s) 691, 1505, 1879, 1977, 2040, 2103, 2166, 2229
AcuI CTGAAG 2 cut(s) 1325, 1815
AcvI CACGTG 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 39, 62
AdeI CACNNNGTG 2 cut(s) 108, 443
AfaI GTAC 2 cut(s) 499, 923
AfeI AGCGCT 1 cut(s) 1112
AfiI CCNNNNNNNGG 6 cut(s) 29, 108, 246, 478, 1133, 1813
AflII CTTAAG 2 cut(s) 1498, 1754
AflIII ACRYGT 1 cut(s) 604
AgeI ACCGGT 1 cut(s) 2263
AgsI TTSAA 8 cut(s) 394, 463, 830, 1166, 1201, 1709, 1850, 2408
AjnI CCWGG 2 cut(s) 1191, 2302
Alw21I GWGCWC 2 cut(s) 850, 898
Alw26I GTCTC 4 cut(s) 27, 128, 226, 1263
Alw44I GTGCAC 2 cut(s) 846, 894
AlwI GGATC 6 cut(s) 238, 770, 945, 1017, 1421, 1951
AlwNI CAGNNNCTG 1 cut(s) 307
Aor51HI AGCGCT 1 cut(s) 1112
AoxI GGCC 7 cut(s) 204, 258, 445, 617, 656, 1274, 2340
ApaLI GTGCAC 2 cut(s) 846, 894
ApeKI GCWGC 6 cut(s) 280, 325, 345, 710, 1371, 1512
ApoI RAATTY 8 cut(s) 691, 1505, 1879, 1977, 2040, 2103, 2166, 2229
ArsI GACNNNNNNTTYG 2 cut(s) 1013, 1045
AsiGI ACCGGT 1 cut(s) 2263
Asp700I GAANNNNTTC 1 cut(s) 467
Asp718I GGTACC 1 cut(s) 921
AspLEI GCGC 9 cut(s) 7, 101, 271, 325, 350, 688, 1113, 1564, 1595
AspS9I GGNCC 3 cut(s) 76, 617, 755
AsuC2I CCSGG 3 cut(s) 173, 248, 1990
AsuII TTCGAA 1 cut(s) 643
AvaII GGWCC 2 cut(s) 76, 755
AxyI CCTNAGG 1 cut(s) 1716
BaeGI GKGCMC 3 cut(s) 173, 850, 898
BalI TGGCCA 1 cut(s) 447
BanI GGYRCC 1 cut(s) 921
BbrPI CACGTG 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 2 cut(s) 850, 898
BbvI GCAGC 6 cut(s) 292, 332, 337, 697, 1358, 1499
BccI CCATC 5 cut(s) 377, 1142, 1351, 1361, 2383
BceAI ACGGC 5 cut(s) 49, 52, 91, 191, 388
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 2 cut(s) 1193, 2304
BciVI GTATCC 1 cut(s) 1086
BclI TGATCA 2 cut(s) 745, 1258
BcnI CCSGG 3 cut(s) 173, 248, 1990
BcoDI GTCTC 4 cut(s) 27, 128, 226, 1263
BfaI CTAG 3 cut(s) 1278, 2298, 2325
BfmI CTRYAG 1 cut(s) 1156
BfoI RGCGCY 3 cut(s) 272, 1114, 1596
BfrI CTTAAG 2 cut(s) 1498, 1754
BfuAI ACCTGC 5 cut(s) 95, 104, 1249, 1297, 1847
BfuI GTATCC 1 cut(s) 1086
BglI GCCNNNNNGGC 1 cut(s) 266
BisI GCNGC 9 cut(s) 240, 267, 281, 326, 346, 711, 1372, 1513, 2284
BlsI GCNGC 9 cut(s) 241, 268, 282, 327, 347, 712, 1373, 1514, 2285
Bme1390I CCNGG 5 cut(s) 173, 248, 1193, 1990, 2304
Bme18I GGWCC 2 cut(s) 76, 755
BmgT120I GGNCC 3 cut(s) 76, 617, 755
BmiI GGNNCC 4 cut(s) 618, 923, 2268, 2371
BmrFI CCNGG 5 cut(s) 173, 248, 1193, 1990, 2304
BmrI ACTGGG 1 cut(s) 172
BmsI GCATC 6 cut(s) 253, 561, 1729, 1843, 1921, 2284
BmuI ACTGGG 1 cut(s) 172
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 4 cut(s) 1097, 1129, 1479, 1511
Bpu10I CCTNAGC 1 cut(s) 1299
Bpu14I TTCGAA 1 cut(s) 643
BpuMI CCSGG 3 cut(s) 173, 248, 1990
BsaAI YACGTR 2 cut(s) 115, 581
BsaBI GATNNNNATC 1 cut(s) 936
BsaHI GRCGYC 2 cut(s) 39, 62
BsaI GGTCTC 1 cut(s) 1263
BsaJI CCNNGG 6 cut(s) 107, 790, 889, 1192, 1247, 2343
BsaWI WCCGGW 6 cut(s) 418, 2011, 2074, 2137, 2200, 2263
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 1324, 1354
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 108, 246, 478, 1133, 1813
Bse118I RCCGGY 7 cut(s) 78, 191, 202, 350, 1209, 1272, 2263
Bse1I ACTGG 2 cut(s) 167, 2270
Bse21I CCTNAGG 1 cut(s) 1716
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 2 cut(s) 1193, 2304
BseDI CCNNGG 6 cut(s) 107, 790, 889, 1192, 1247, 2343
BseGI GGATG 4 cut(s) 388, 522, 1744, 1972
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 6 cut(s) 29, 108, 246, 478, 1133, 1813
BseMII CTCAG 6 cut(s) 603, 669, 771, 1119, 1290, 1730
BseNI ACTGG 2 cut(s) 167, 2270
BseRI GAGGAG 6 cut(s) 1105, 1345, 2042, 2105, 2168, 2231
BseSI GKGCMC 3 cut(s) 173, 850, 898
BseX3I CGGCCG 1 cut(s) 204
BseXI GCAGC 6 cut(s) 292, 332, 337, 697, 1358, 1499
BseYI CCCAGC 1 cut(s) 813
BsgI GTGCAG 1 cut(s) 1227
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 2 cut(s) 207, 255
BshFI GGCC 7 cut(s) 206, 260, 447, 619, 658, 1276, 2342
BshNI GGYRCC 1 cut(s) 921
BshTI ACCGGT 1 cut(s) 2263
BsiEI CGRYCG 2 cut(s) 207, 255
BsiHKAI GWGCWC 2 cut(s) 850, 898
BslFI GGGAC 4 cut(s) 115, 120, 1364, 1896
BslI CCNNNNNNNGG 6 cut(s) 29, 108, 246, 478, 1133, 1813
BsmAI GTCTC 4 cut(s) 27, 128, 226, 1263
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 4 cut(s) 115, 120, 1364, 1896
BsmI GAATGC 1 cut(s) 815
BsnI GGCC 7 cut(s) 206, 260, 447, 619, 658, 1276, 2342
Bso31I GGTCTC 1 cut(s) 1263
Bsp119I TTCGAA 1 cut(s) 643
Bsp1286I GDGCHC 3 cut(s) 173, 850, 898
Bsp19I CCATGG 2 cut(s) 889, 1247
BspACI CCGC 8 cut(s) 240, 266, 315, 550, 682, 1141, 1524, 2283
BspANI GGCC 7 cut(s) 206, 260, 447, 619, 658, 1276, 2342
BspCNI CTCAG 6 cut(s) 604, 670, 772, 1120, 1291, 1729
BspFNI CGCG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 1533
BspLI GGNNCC 4 cut(s) 618, 923, 2268, 2371
BspMI ACCTGC 5 cut(s) 95, 104, 1249, 1297, 1847
BspPI GGATC 6 cut(s) 238, 770, 945, 1017, 1421, 1951
BspT104I TTCGAA 1 cut(s) 643
BspT107I GGYRCC 1 cut(s) 921
BspTI CTTAAG 2 cut(s) 1498, 1754
BspTNI GGTCTC 1 cut(s) 1263
BsrBI CCGCTC 2 cut(s) 315, 682
BsrFI RCCGGY 7 cut(s) 78, 191, 202, 350, 1209, 1272, 2263
BsrI ACTGG 2 cut(s) 167, 2270
BssAI RCCGGY 7 cut(s) 78, 191, 202, 350, 1209, 1272, 2263
BssECI CCNNGG 6 cut(s) 107, 790, 889, 1192, 1247, 2343
BssNI GRCGYC 2 cut(s) 39, 62
BssT1I CCWWGG 3 cut(s) 790, 889, 1247
Bst2UI CCWGG 2 cut(s) 1193, 2304
Bst4CI ACNGT 6 cut(s) 179, 840, 882, 900, 1414, 1483
Bst6I CTCTTC 3 cut(s) 1447, 1764, 1770
BstACI GRCGYC 2 cut(s) 39, 62
BstAFI CTTAAG 2 cut(s) 1498, 1754
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstBAI YACGTR 2 cut(s) 115, 581
BstBI TTCGAA 1 cut(s) 643
BstC8I GCNNGC 7 cut(s) 47, 147, 204, 352, 621, 684, 2288
BstDEI CTNAG 6 cut(s) 612, 678, 780, 1128, 1299, 1716
BstDSI CCRYGG 3 cut(s) 107, 889, 1247
BstENI CCTNNNNNAGG 1 cut(s) 1131
BstF5I GGATG 4 cut(s) 388, 522, 1744, 1972
BstFNI CGCG 1 cut(s) 242
BstH2I RGCGCY 3 cut(s) 272, 1114, 1596
BstHHI GCGC 9 cut(s) 7, 101, 271, 325, 350, 688, 1113, 1564, 1595
BstMAI GTCTC 4 cut(s) 27, 128, 226, 1263
BstMCI CGRYCG 2 cut(s) 207, 255
BstNI CCWGG 2 cut(s) 1193, 2304
BstNSI RCATGY 2 cut(s) 49, 608
BstSCI CCNGG 5 cut(s) 171, 246, 1191, 1988, 2302
BstSFI CTRYAG 1 cut(s) 1156
BstSLI GKGCMC 3 cut(s) 173, 850, 898
BstSNI TACGTA 1 cut(s) 581
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 6 cut(s) 292, 332, 337, 697, 1358, 1499
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 1426
BstXI CCANNNNNNTGG 6 cut(s) 583, 866, 2060, 2123, 2186, 2249
BstYI RGATCY 1 cut(s) 1426
BstZI CGGCCG 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 1716
BsuI GTATCC 1 cut(s) 1086
BsuRI GGCC 7 cut(s) 206, 260, 447, 619, 658, 1276, 2342
BtgI CCRYGG 3 cut(s) 107, 889, 1247
BtsCI GGATG 4 cut(s) 388, 522, 1744, 1972
BtsIMutI CAGTG 4 cut(s) 160, 845, 1686, 1836
BveI ACCTGC 5 cut(s) 95, 104, 1249, 1297, 1847
Cac8I GCNNGC 7 cut(s) 47, 147, 204, 352, 621, 684, 2288
CaiI CAGNNNCTG 1 cut(s) 307
CciI TCATGA 1 cut(s) 1533
CfoI GCGC 9 cut(s) 7, 101, 271, 325, 350, 688, 1113, 1564, 1595
Cfr10I RCCGGY 7 cut(s) 78, 191, 202, 350, 1209, 1272, 2263
Cfr13I GGNCC 3 cut(s) 76, 617, 755
CseI GACGC 2 cut(s) 28, 70
Csp6I GTAC 2 cut(s) 498, 922
CspAI ACCGGT 1 cut(s) 2263
CviQI GTAC 2 cut(s) 498, 922
DdeI CTNAG 6 cut(s) 612, 678, 780, 1128, 1299, 1716
DraIII CACNNNGTG 2 cut(s) 108, 443
EaeI YGGCCR 2 cut(s) 204, 445
EagI CGGCCG 1 cut(s) 204
Eam1104I CTCTTC 3 cut(s) 1447, 1764, 1770
EarI CTCTTC 3 cut(s) 1447, 1764, 1770
EclXI CGGCCG 1 cut(s) 204
Eco105I TACGTA 1 cut(s) 581
Eco130I CCWWGG 3 cut(s) 790, 889, 1247
Eco147I AGGCCT 1 cut(s) 658
Eco31I GGTCTC 1 cut(s) 1263
Eco32I GATATC 1 cut(s) 1693
Eco47I GGWCC 2 cut(s) 76, 755
Eco47III AGCGCT 1 cut(s) 1112
Eco52I CGGCCG 1 cut(s) 204
Eco57I CTGAAG 2 cut(s) 1325, 1815
Eco72I CACGTG 1 cut(s) 115
Eco81I CCTNAGG 1 cut(s) 1716
EcoNI CCTNNNNNAGG 1 cut(s) 1131
EcoO109I RGGNCCY 1 cut(s) 617
EcoRI GAATTC 5 cut(s) 1505, 1879, 2040, 2103, 2166
EcoRII CCWGG 2 cut(s) 1191, 2302
EcoRV GATATC 1 cut(s) 1693
EcoT14I CCWWGG 3 cut(s) 790, 889, 1247
ErhI CCWWGG 3 cut(s) 790, 889, 1247
Esp3I CGTCTC 1 cut(s) 27
FaqI GGGAC 4 cut(s) 115, 120, 1364, 1896
FauI CCCGC 4 cut(s) 308, 557, 675, 1517
FauNDI CATATG 1 cut(s) 1120
FbaI TGATCA 2 cut(s) 745, 1258
FblI GTMKAC 1 cut(s) 835
Fnu4HI GCNGC 9 cut(s) 240, 267, 281, 326, 346, 711, 1372, 1513, 2284
FokI GGATG 4 cut(s) 395, 509, 1751, 1979
Fsp4HI GCNGC 9 cut(s) 240, 267, 281, 326, 346, 711, 1372, 1513, 2284
FspAI RTGCGCAY 1 cut(s) 1563
FspBI CTAG 3 cut(s) 1278, 2298, 2325
FspI TGCGCA 1 cut(s) 1563
GlaI GCGC 9 cut(s) 6, 100, 270, 324, 349, 687, 1112, 1563, 1594
GluI GCNGC 9 cut(s) 240, 267, 281, 326, 346, 711, 1372, 1513, 2284
GsaI CCCAGC 1 cut(s) 817
HaeII RGCGCY 3 cut(s) 272, 1114, 1596
HaeIII GGCC 7 cut(s) 206, 260, 447, 619, 658, 1276, 2342
HgaI GACGC 2 cut(s) 28, 70
HhaI GCGC 9 cut(s) 7, 101, 271, 325, 350, 688, 1113, 1564, 1595
Hin1I GRCGYC 2 cut(s) 39, 62
Hin6I GCGC 9 cut(s) 5, 99, 269, 323, 348, 686, 1111, 1562, 1593
HinP1I GCGC 9 cut(s) 5, 99, 269, 323, 348, 686, 1111, 1562, 1593
HincII GTYRAC 2 cut(s) 545, 836
HindII GTYRAC 2 cut(s) 545, 836
HindIII AAGCTT 1 cut(s) 666
HinfI GANTC 5 cut(s) 140, 1152, 1188, 1787, 1909
Hpy166II GTNNAC 6 cut(s) 367, 545, 836, 848, 896, 1723
Hpy188I TCNGA 8 cut(s) 70, 1419, 1487, 1649, 1699, 1825, 1903, 2401
Hpy188III TCNNGA 8 cut(s) 137, 472, 696, 779, 915, 1430, 1449, 1534
Hpy8I GTNNAC 6 cut(s) 367, 545, 836, 848, 896, 1723
Hpy99I CGWCG 4 cut(s) 32, 64, 70, 374
HpyCH4III ACNGT 6 cut(s) 179, 840, 882, 900, 1414, 1483
HpyCH4IV ACGT 4 cut(s) 9, 114, 580, 1421
HpyF3I CTNAG 6 cut(s) 612, 678, 780, 1128, 1299, 1716
HpySE526I ACGT 4 cut(s) 9, 114, 580, 1421
Hsp92I GRCGYC 2 cut(s) 39, 62
HspAI GCGC 9 cut(s) 5, 99, 269, 323, 348, 686, 1111, 1562, 1593
KpnI GGTACC 1 cut(s) 925
KroI GCCGGC 2 cut(s) 202, 350
KroNI GCCGGC 2 cut(s) 204, 352
Ksp22I TGATCA 2 cut(s) 745, 1258
LmnI GCTCC 4 cut(s) 277, 304, 421, 1118
Lsp1109I GCAGC 6 cut(s) 292, 332, 337, 697, 1358, 1499
LweI GCATC 6 cut(s) 253, 561, 1729, 1843, 1921, 2284
MaeI CTAG 3 cut(s) 1278, 2298, 2325
MaeII ACGT 4 cut(s) 9, 114, 580, 1421
MbiI CCGCTC 2 cut(s) 315, 682
MfeI CAATTG 1 cut(s) 394
MflI RGATCY 1 cut(s) 1426
MhlI GDGCHC 3 cut(s) 173, 850, 898
MlsI TGGCCA 1 cut(s) 447
MluNI TGGCCA 1 cut(s) 447
MlyI GAGTC 1 cut(s) 1918
MmeI TCCRAC 3 cut(s) 48, 463, 1154
Mox20I TGGCCA 1 cut(s) 447
MroNI GCCGGC 2 cut(s) 202, 350
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 447
MseI TTAA 4 cut(s) 1499, 1733, 1755, 2313
MslI CAYNNNNRTG 5 cut(s) 581, 2058, 2121, 2184, 2247
Msp20I TGGCCA 1 cut(s) 447
MspA1I CMGCKG 3 cut(s) 743, 1512, 1793
MspCI CTTAAG 2 cut(s) 1498, 1754
MspR9I CCNGG 5 cut(s) 173, 248, 1193, 1990, 2304
MunI CAATTG 1 cut(s) 394
Mva1269I GAATGC 1 cut(s) 815
MvaI CCWGG 2 cut(s) 1193, 2304
MvnI CGCG 1 cut(s) 242
NaeI GCCGGC 2 cut(s) 204, 352
NciI CCSGG 3 cut(s) 173, 248, 1990
NcoI CCATGG 2 cut(s) 889, 1247
NdeI CATATG 1 cut(s) 1120
NgoMIV GCCGGC 2 cut(s) 202, 350
NlaIV GGNNCC 4 cut(s) 618, 923, 2268, 2371
NmeAIII GCCGAG 1 cut(s) 2368
NmuCI GTSAC 9 cut(s) 430, 730, 1229, 1749, 1758, 2047, 2110, 2173, 2236
NsbI TGCGCA 1 cut(s) 1563
NspI RCATGY 2 cut(s) 49, 608
NspV TTCGAA 1 cut(s) 643
PaeI GCATGC 1 cut(s) 49
PagI TCATGA 1 cut(s) 1533
PaqCI CACCTGC 2 cut(s) 95, 1847
PceI AGGCCT 1 cut(s) 658
PciI ACATGT 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 815
PdiI GCCGGC 2 cut(s) 204, 352
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 4 cut(s) 140, 1152, 1188, 1787
PflFI GACNNNGTC 2 cut(s) 38, 64
PflMI CCANNNNNTGG 2 cut(s) 108, 1813
PinAI ACCGGT 1 cut(s) 2263
PkrI GCNGC 9 cut(s) 241, 268, 282, 327, 347, 712, 1373, 1514, 2285
Ple19I CGATCG 1 cut(s) 255
PleI GAGTC 1 cut(s) 1917
PmaCI CACGTG 1 cut(s) 115
PmlI CACGTG 1 cut(s) 115
PpsI GAGTC 1 cut(s) 1917
Ppu21I YACGTR 2 cut(s) 115, 581
PscI ACATGT 1 cut(s) 604
Psp6I CCWGG 2 cut(s) 1191, 2302
PspCI CACGTG 1 cut(s) 115
PspFI CCCAGC 1 cut(s) 813
PspGI CCWGG 2 cut(s) 1191, 2302
PspN4I GGNNCC 4 cut(s) 618, 923, 2268, 2371
PspPI GGNCC 3 cut(s) 76, 617, 755
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 1426
PsyI GACNNNGTC 2 cut(s) 38, 64
PvuI CGATCG 1 cut(s) 255
PvuII CAGCTG 3 cut(s) 743, 1512, 1793
RsaI GTAC 2 cut(s) 499, 923
RsaNI GTAC 2 cut(s) 498, 922
RseI CAYNNNNRTG 5 cut(s) 581, 2058, 2121, 2184, 2247
SalI GTCGAC 1 cut(s) 834
SaqAI TTAA 4 cut(s) 1499, 1733, 1755, 2313
SatI GCNGC 9 cut(s) 240, 267, 281, 326, 346, 711, 1372, 1513, 2284
Sau96I GGNCC 3 cut(s) 76, 617, 755
SchI GAGTC 1 cut(s) 1918
ScrFI CCNGG 5 cut(s) 173, 248, 1193, 1990, 2304
SduI GDGCHC 3 cut(s) 173, 850, 898
SfaNI GCATC 6 cut(s) 253, 561, 1729, 1843, 1921, 2284
SfcI CTRYAG 1 cut(s) 1156
SfuI TTCGAA 1 cut(s) 643
SgrAI CRCCGGYG 1 cut(s) 191
SinI GGWCC 2 cut(s) 76, 755
SmiMI CAYNNNNRTG 5 cut(s) 581, 2058, 2121, 2184, 2247
SmlI CTYRAG 2 cut(s) 1498, 1754
SmoI CTYRAG 2 cut(s) 1498, 1754
SnaBI TACGTA 1 cut(s) 581
SphI GCATGC 1 cut(s) 49
SseBI AGGCCT 1 cut(s) 658
SsiI CCGC 8 cut(s) 240, 266, 315, 550, 682, 1141, 1524, 2283
SspMI CTAG 3 cut(s) 1278, 2298, 2325
StuI AGGCCT 1 cut(s) 658
StyD4I CCNGG 5 cut(s) 171, 246, 1191, 1988, 2302
StyI CCWWGG 3 cut(s) 790, 889, 1247
TaaI ACNGT 6 cut(s) 179, 840, 882, 900, 1414, 1483
TaiI ACGT 4 cut(s) 12, 117, 583, 1424
TaqI TCGA 4 cut(s) 255, 643, 835, 1438
TauI GCSGC 3 cut(s) 242, 269, 2286
TfiI GAWTC 4 cut(s) 140, 1152, 1188, 1787
Tru1I TTAA 4 cut(s) 1499, 1733, 1755, 2313
Tru9I TTAA 4 cut(s) 1499, 1733, 1755, 2313
TscAI CASTG 4 cut(s) 167, 845, 1693, 1843
TseFI GTSAC 9 cut(s) 430, 730, 1229, 1749, 1758, 2047, 2110, 2173, 2236
TseI GCWGC 6 cut(s) 280, 325, 345, 710, 1371, 1512
Tsp45I GTSAC 9 cut(s) 430, 730, 1229, 1749, 1758, 2047, 2110, 2173, 2236
TspDTI ATGAA 8 cut(s) 1082, 1251, 1989, 2033, 2096, 2159, 2241, 2351
TspGWI ACGGA 3 cut(s) 45, 290, 1281
TspRI CASTG 4 cut(s) 167, 845, 1693, 1843
Tth111I GACNNNGTC 2 cut(s) 38, 64
Van91I CCANNNNNTGG 2 cut(s) 108, 1813
Vha464I CTTAAG 2 cut(s) 1498, 1754
VneI GTGCAC 2 cut(s) 846, 894
VpaK11BI GGWCC 2 cut(s) 76, 755
XagI CCTNNNNNAGG 1 cut(s) 1131
XapI RAATTY 8 cut(s) 691, 1505, 1879, 1977, 2040, 2103, 2166, 2229
XceI RCATGY 2 cut(s) 49, 608
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XmiI GTMKAC 1 cut(s) 835
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 3 cut(s) 1278, 2298, 2325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.