Rh7CG099400

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000644
Physical Location & Seq
Forward (+)
7446913 .. 7452220
5308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG099400.1

Sequence Viewer

Length: 1923 bp
ATGTACCATCTAAGGAAAAAGGCGTTTTTGCTGACACTGGAAGCTTTGTACAACCTTTTGATCCAATATGTAGGTTGGTATTCTTCTTGGTCCACATGGTTGTGGGCACAGAAATCTACAAGGGTGATTTTATATACACCATGTGGTCACAATTTCTGCTTGAAGTGCTTCCAGAAATGGGTTGGACAAGGGAAGCGTACTTGTGCAAAGTGCCGCCATGCCATTCCTTCCAAAATGGCAAGTCAACCCCGCATCAATTCTTCACTTGTAGCTGCCATACGTATGGCTAAGATGTCAAAATCATATGCTACTGAGGGGCCTGCTAAGGTATACCACTTTGTCCGAAACCAAGACAGACCTGACAAAGCTTTCACTACCGAGCGGGCGCAGAAAACTGGGAAATCTAATGCTGCAAGTGGCAAGATATTTGTCACAATTCCAGCTGATCATTTTGGACCTATACCTGCTGAATATGATCCTGAGAGAAACCAAGGTGTGCTGGTAGGGGAATGCTGGGAGGATAGGCTTGAATGTCGACAGTGGGGTGCACACTTTCCCCATGTTGCTGGTATAGCTGGACAGTCTAACCATGGTGCACAGTCAGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATACACAGGAAGTGGTGGAAGGGATTTGAGTGGGAATAAGCGAACAAACAAGGATCAGTCATTTGACCAGACATTTGAGAAATATAACAAGGCTCTCCAAGTTAGTTGCCTGAAAGGGTATCCTGTACGGGTGGTAAGATCGCACAAGGAGAAGCGCTCCTCATATGCCCCTGAGAAAGGGTTGCGGTATGATGGAGTCTATAGAATTGAAAAATGTTGGACAAAGGTGGGAATCCAGGGGTTTAAGGTCTGCCGGTATCTTTTTGTTAGATGTGACAATGAACCTGCACCATGGACGAGTGATGAACATGGGGACCGGCCTAGACCTTTACCTCCAATACCTGAGCTGAAGAAAGCTACAAGTATAATAGAGAGGAAGTCAAGTCCATCGTGGGACTTTGAGGAGAAAGATGGCTGCTGGAAGTGGAAAAAGTCACCACCTATCAGTAAAAAAACCGTTCAGACATTAGATGCTGCTGATCGAAAGAGGTCAAGAAAAGGAAAGAGGAAGGCAAAAAATATTACTGTCCGAGAGAGGCTCTTAAGAGAATTCAGCTGCCTTGTTTGCGGGGAGGTCATGACATTACCAATGACCACACCTTGTGCTCATAACTTTTGCAAATCCTGTTTGGAAGGCGCTTTTGCGGGAAAGAGCTTGGTGAGAGAAAGAAACAGAGGGGGAAGAACCCTTCGAACCCAAAAGAATATAATGAGCTGCCCTTCTTGTCCTACTGATATCTCTGATTTTCTTCAAGATCCTCAGGTGAATAGAGAGTTAATGGATGCGATTGAGTCACTTAAGTCACAGAATGAAGAGGAAGAGAATGAGGATTCAGCTGAAGAAATGAGTGAAGATGTGGATAATTCTGACAACGAAATTAGCACTGAAATGGTTGAAGAAGCAGGTGTTAAAGAATGTGCTGAGGGTTCTCCAACAAACTGCAATCCCGAAAGGAGTCCCAAACGACAGAAAGTTGATGCTGGTGAAGGCTCACCAAAAACCAATGATGAGGAGGTAAATTGCATTCCTGTCGCCCAGACTAATGGCAAAGGCTCACCGGAAACCGATACTGAAGAGGTAAATGAAACTCCTGTCGCCCATACTAATGGCAAAGGCTCACCGGCTCCAGTTGCAAAATGTGGCAAGCAAGATGCTGGTCCGGTTGGCGTTGCAGCAAATACTAGGAGGAGGAAGAAGGCTGAAGTAGTGGGTAAAGATAATGGTTCCCCATCAAGTCCTCTGCTTGTGCAATCTGATTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

640

Amino Acids

71.58

Weight (kDa)

8.75

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 40 - 72 7.2e-06 Ring finger domain
SAD_SRA PF02182 163 - 312 1.1e-49 SAD/SRA domain
zf-RING_UBOX PF13445 405 - 436 4e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1550
Acc36I ACCTGC 3 cut(s) 472, 949, 1550
Acc65I GGTACC 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 621
AccBSI CCGCTC 1 cut(s) 382
AccI GTMKAC 2 cut(s) 330, 535
AciI CCGC 6 cut(s) 214, 250, 382, 841, 1224, 1301
AclWI GGATC 5 cut(s) 55, 470, 645, 717, 1406
AcsI RAATTY 1 cut(s) 1205
AcuI CTGAAG 4 cut(s) 1025, 1515, 1749, 1878
AdeI CACNNNGTG 2 cut(s) 143, 1259
AfaI GTAC 5 cut(s) 5, 50, 199, 623, 783
AfeI AGCGCT 1 cut(s) 812
AfiI CCNNNNNNNGG 2 cut(s) 178, 833
AflII CTTAAG 2 cut(s) 1198, 1454
AgsI TTSAA 6 cut(s) 163, 530, 866, 1409, 1553, 1919
AjnI CCWGG 1 cut(s) 891
Alw21I GWGCWC 3 cut(s) 550, 598, 1264
Alw44I GTGCAC 2 cut(s) 546, 594
AlwI GGATC 5 cut(s) 55, 470, 645, 717, 1406
Aor51HI AGCGCT 1 cut(s) 812
AoxI GGCC 2 cut(s) 317, 974
ApaLI GTGCAC 2 cut(s) 546, 594
ApeKI GCWGC 7 cut(s) 272, 410, 1071, 1130, 1212, 1371, 1829
ApoI RAATTY 1 cut(s) 1205
ArsI GACNNNNNNTTYG 2 cut(s) 713, 745
Asp700I GAANNNNTTC 1 cut(s) 167
Asp718I GGTACC 1 cut(s) 621
AspLEI GCGC 3 cut(s) 388, 813, 1295
AspS9I GGNCC 5 cut(s) 90, 317, 455, 970, 1814
AsuHPI GGTGA 8 cut(s) 136, 1083, 1327, 1432, 1641, 1652, 1704, 1767
AsuII TTCGAA 1 cut(s) 1348
AvaII GGWCC 4 cut(s) 90, 455, 970, 1814
AxyI CCTNAGG 1 cut(s) 1416
BaeGI GKGCMC 3 cut(s) 109, 550, 598
BanI GGYRCC 1 cut(s) 621
Bbv12I GWGCWC 3 cut(s) 550, 598, 1264
BbvCI CCTCAGC 1 cut(s) 1578
BbvI GCAGC 7 cut(s) 259, 397, 1058, 1117, 1199, 1358, 1841
BccI CCATC 5 cut(s) 15, 842, 1051, 1061, 1894
BcgI CGANNNNNNTGC 2 cut(s) 1669, 1703
BciT130I CCWGG 1 cut(s) 893
BciVI GTATCC 1 cut(s) 786
BclI TGATCA 1 cut(s) 445
BfaI CTAG 2 cut(s) 978, 1839
BfmI CTRYAG 1 cut(s) 856
BfoI RGCGCY 2 cut(s) 814, 1296
BfrI CTTAAG 2 cut(s) 1198, 1454
BfuAI ACCTGC 3 cut(s) 472, 949, 1550
BfuI GTATCC 1 cut(s) 786
BisI GCNGC 8 cut(s) 214, 273, 411, 1072, 1131, 1213, 1372, 1830
BlsI GCNGC 8 cut(s) 215, 274, 412, 1073, 1132, 1214, 1373, 1831
Bme1390I CCNGG 1 cut(s) 893
Bme18I GGWCC 4 cut(s) 90, 455, 970, 1814
BmgT120I GGNCC 5 cut(s) 90, 317, 455, 970, 1814
BmiI GGNNCC 5 cut(s) 318, 623, 971, 1782, 1882
BmrFI CCNGG 1 cut(s) 893
BmrI ACTGGG 1 cut(s) 405
BmsI GCATC 5 cut(s) 261, 1117, 1429, 1624, 1798
BmuI ACTGGG 1 cut(s) 405
BplI GAGNNNNNCTC 4 cut(s) 797, 829, 1179, 1211
BpmI CTGGAG 1 cut(s) 1767
Bpu10I CCTNAGC 3 cut(s) 324, 999, 1578
Bpu14I TTCGAA 1 cut(s) 1348
BsaAI YACGTR 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 490, 589, 892, 947
BsaWI WCCGGW 2 cut(s) 1714, 1816
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 833
Bse118I RCCGGY 3 cut(s) 909, 972, 1777
Bse1I ACTGG 3 cut(s) 42, 400, 1784
Bse21I CCTNAGG 1 cut(s) 1416
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 1 cut(s) 893
BseDI CCNNGG 4 cut(s) 490, 589, 892, 947
BseGI GGATG 1 cut(s) 1444
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 2 cut(s) 178, 833
BseMII CTCAG 6 cut(s) 303, 471, 819, 990, 1430, 1569
BseNI ACTGG 3 cut(s) 42, 400, 1784
BseRI GAGGAG 4 cut(s) 805, 1073, 1682, 1858
BseSI GKGCMC 3 cut(s) 109, 550, 598
BseXI GCAGC 7 cut(s) 259, 397, 1058, 1117, 1199, 1358, 1841
BseYI CCCAGC 1 cut(s) 513
BsgI GTGCAG 1 cut(s) 927
BshFI GGCC 2 cut(s) 319, 976
BshNI GGYRCC 1 cut(s) 621
BsiHKAI GWGCWC 3 cut(s) 550, 598, 1264
BsiSI CCGG 5 cut(s) 910, 973, 1715, 1778, 1817
BslFI GGGAC 3 cut(s) 983, 1064, 1599
BslI CCNNNNNNNGG 2 cut(s) 178, 833
BsmFI GGGAC 3 cut(s) 983, 1064, 1599
BsmI GAATGC 2 cut(s) 515, 1680
BsnI GGCC 2 cut(s) 319, 976
Bsp119I TTCGAA 1 cut(s) 1348
Bsp1286I GDGCHC 4 cut(s) 109, 550, 598, 1264
Bsp1407I TGTACA 1 cut(s) 48
Bsp143I GATC 8 cut(s) 60, 445, 475, 637, 709, 794, 1135, 1411
Bsp19I CCATGG 2 cut(s) 589, 947
BspACI CCGC 6 cut(s) 214, 250, 382, 841, 1224, 1301
BspANI GGCC 2 cut(s) 319, 976
BspCNI CTCAG 6 cut(s) 304, 472, 820, 991, 1429, 1570
BspHI TCATGA 1 cut(s) 1233
BspLI GGNNCC 5 cut(s) 318, 623, 971, 1782, 1882
BspMI ACCTGC 3 cut(s) 472, 949, 1550
BspPI GGATC 5 cut(s) 55, 470, 645, 717, 1406
BspT104I TTCGAA 1 cut(s) 1348
BspT107I GGYRCC 1 cut(s) 621
BspTI CTTAAG 2 cut(s) 1198, 1454
BsrBI CCGCTC 1 cut(s) 382
BsrFI RCCGGY 3 cut(s) 909, 972, 1777
BsrGI TGTACA 1 cut(s) 48
BsrI ACTGG 3 cut(s) 42, 400, 1784
BssAI RCCGGY 3 cut(s) 909, 972, 1777
BssECI CCNNGG 4 cut(s) 490, 589, 892, 947
BssMI GATC 8 cut(s) 60, 445, 475, 637, 709, 794, 1135, 1411
BssNAI GTATAC 1 cut(s) 331
BssT1I CCWWGG 3 cut(s) 490, 589, 947
Bst1107I GTATAC 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 893
Bst4CI ACNGT 5 cut(s) 540, 582, 600, 1114, 1183
Bst6I CTCTTC 3 cut(s) 1464, 1470, 1725
BstAFI CTTAAG 2 cut(s) 1198, 1454
BstAUI TGTACA 1 cut(s) 48
BstBAI YACGTR 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 1348
BstC8I GCNNGC 3 cut(s) 321, 384, 1802
BstDEI CTNAG 9 cut(s) 11, 288, 312, 324, 480, 828, 999, 1416, 1578
BstDSI CCRYGG 2 cut(s) 589, 947
BstENI CCTNNNNNAGG 1 cut(s) 831
BstF5I GGATG 1 cut(s) 1444
BstH2I RGCGCY 2 cut(s) 814, 1296
BstHHI GCGC 3 cut(s) 388, 813, 1295
BstKTI GATC 8 cut(s) 63, 448, 478, 640, 712, 797, 1138, 1414
BstMBI GATC 8 cut(s) 60, 445, 475, 637, 709, 794, 1135, 1411
BstMWI GCNNNNNNNGC 4 cut(s) 165, 572, 1221, 1787
BstNI CCWGG 1 cut(s) 893
BstSCI CCNGG 1 cut(s) 891
BstSFI CTRYAG 1 cut(s) 856
BstSLI GKGCMC 3 cut(s) 109, 550, 598
BstSNI TACGTA 1 cut(s) 281
BstV1I GCAGC 7 cut(s) 259, 397, 1058, 1117, 1199, 1358, 1841
BstX2I RGATCY 1 cut(s) 1411
BstXI CCANNNNNNTGG 4 cut(s) 283, 566, 1700, 1763
BstYI RGATCY 1 cut(s) 1411
BstZ17I GTATAC 1 cut(s) 331
Bsu36I CCTNAGG 1 cut(s) 1416
BsuI GTATCC 1 cut(s) 786
BsuRI GGCC 2 cut(s) 319, 976
BtgI CCRYGG 2 cut(s) 589, 947
BtsCI GGATG 1 cut(s) 1444
BtsIMutI CAGTG 3 cut(s) 35, 545, 1539
BveI ACCTGC 3 cut(s) 472, 949, 1550
Cac8I GCNNGC 3 cut(s) 321, 384, 1802
CciI TCATGA 1 cut(s) 1233
CfoI GCGC 3 cut(s) 388, 813, 1295
Cfr10I RCCGGY 3 cut(s) 909, 972, 1777
Cfr13I GGNCC 5 cut(s) 90, 317, 455, 970, 1814
Csp6I GTAC 5 cut(s) 4, 49, 198, 622, 782
CviAII CATG 9 cut(s) 96, 141, 218, 560, 590, 641, 948, 965, 1234
CviQI GTAC 5 cut(s) 4, 49, 198, 622, 782
DdeI CTNAG 9 cut(s) 11, 288, 312, 324, 480, 828, 999, 1416, 1578
DpnI GATC 8 cut(s) 62, 447, 477, 639, 711, 796, 1137, 1413
DpnII GATC 8 cut(s) 60, 445, 475, 637, 709, 794, 1135, 1411
DraIII CACNNNGTG 2 cut(s) 143, 1259
Eam1104I CTCTTC 3 cut(s) 1464, 1470, 1725
EarI CTCTTC 3 cut(s) 1464, 1470, 1725
Eco105I TACGTA 1 cut(s) 281
Eco130I CCWWGG 3 cut(s) 490, 589, 947
Eco32I GATATC 1 cut(s) 1393
Eco47I GGWCC 4 cut(s) 90, 455, 970, 1814
Eco47III AGCGCT 1 cut(s) 812
Eco57I CTGAAG 4 cut(s) 1025, 1515, 1749, 1878
Eco81I CCTNAGG 1 cut(s) 1416
EcoNI CCTNNNNNAGG 1 cut(s) 831
EcoO109I RGGNCCY 1 cut(s) 317
EcoRI GAATTC 1 cut(s) 1205
EcoRII CCWGG 1 cut(s) 891
EcoRV GATATC 1 cut(s) 1393
EcoT14I CCWWGG 3 cut(s) 490, 589, 947
ErhI CCWWGG 3 cut(s) 490, 589, 947
FaeI CATG 9 cut(s) 99, 144, 221, 563, 593, 644, 951, 968, 1237
FaqI GGGAC 3 cut(s) 983, 1064, 1599
FatI CATG 9 cut(s) 95, 140, 217, 559, 589, 640, 947, 964, 1233
FauI CCCGC 4 cut(s) 257, 375, 1217, 1294
FauNDI CATATG 2 cut(s) 304, 820
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 2 cut(s) 330, 535
Fnu4HI GCNGC 8 cut(s) 214, 273, 411, 1072, 1131, 1213, 1372, 1830
FokI GGATG 1 cut(s) 1451
Fsp4HI GCNGC 8 cut(s) 214, 273, 411, 1072, 1131, 1213, 1372, 1830
FspBI CTAG 2 cut(s) 978, 1839
GlaI GCGC 3 cut(s) 387, 812, 1294
GluI GCNGC 8 cut(s) 214, 273, 411, 1072, 1131, 1213, 1372, 1830
GsaI CCCAGC 1 cut(s) 517
GsuI CTGGAG 1 cut(s) 1767
HaeII RGCGCY 2 cut(s) 814, 1296
HaeIII GGCC 2 cut(s) 319, 976
HapII CCGG 5 cut(s) 910, 973, 1715, 1778, 1817
HhaI GCGC 3 cut(s) 388, 813, 1295
Hin1II CATG 9 cut(s) 99, 144, 221, 563, 593, 644, 951, 968, 1237
Hin6I GCGC 3 cut(s) 386, 811, 1293
HinP1I GCGC 3 cut(s) 386, 811, 1293
HincII GTYRAC 2 cut(s) 245, 536
HindII GTYRAC 2 cut(s) 245, 536
HindIII AAGCTT 2 cut(s) 42, 366
HinfI GANTC 5 cut(s) 852, 888, 1448, 1487, 1612
HpaII CCGG 5 cut(s) 910, 973, 1715, 1778, 1817
HphI GGTGA 8 cut(s) 136, 1083, 1327, 1432, 1641, 1652, 1704, 1767
Hpy166II GTNNAC 6 cut(s) 93, 245, 331, 536, 548, 596
Hpy188I TCNGA 6 cut(s) 344, 1119, 1187, 1399, 1525, 1912
Hpy188III TCNNGA 7 cut(s) 172, 479, 615, 1149, 1234, 1409, 1604
Hpy8I GTNNAC 6 cut(s) 93, 245, 331, 536, 548, 596
HpyAV CCTTC 8 cut(s) 237, 669, 1159, 1283, 1355, 1386, 1637, 1846
HpyCH4III ACNGT 5 cut(s) 540, 582, 600, 1114, 1183
HpyCH4IV ACGT 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 572, 1221, 1787
HpyF3I CTNAG 9 cut(s) 11, 288, 312, 324, 480, 828, 999, 1416, 1578
HpySE526I ACGT 1 cut(s) 280
Hsp92II CATG 9 cut(s) 99, 144, 221, 563, 593, 644, 951, 968, 1237
HspAI GCGC 3 cut(s) 386, 811, 1293
KpnI GGTACC 1 cut(s) 625
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 8 cut(s) 60, 445, 475, 637, 709, 794, 1135, 1411
LmnI GCTCC 2 cut(s) 818, 1786
Lsp1109I GCAGC 7 cut(s) 259, 397, 1058, 1117, 1199, 1358, 1841
LweI GCATC 5 cut(s) 261, 1117, 1429, 1624, 1798
MaeI CTAG 2 cut(s) 978, 1839
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 6 cut(s) 146, 430, 929, 1089, 1449, 1458
MalI GATC 8 cut(s) 62, 447, 477, 639, 711, 796, 1137, 1413
MbiI CCGCTC 1 cut(s) 382
MboI GATC 8 cut(s) 60, 445, 475, 637, 709, 794, 1135, 1411
MflI RGATCY 1 cut(s) 1411
MhlI GDGCHC 4 cut(s) 109, 550, 598, 1264
MluCI AATT 8 cut(s) 151, 256, 435, 861, 1205, 1519, 1533, 1675
MlyI GAGTC 3 cut(s) 861, 1457, 1621
MmeI TCCRAC 3 cut(s) 163, 854, 1613
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 5 cut(s) 900, 1199, 1433, 1455, 1566
MslI CAYNNNNRTG 4 cut(s) 100, 281, 1544, 1761
MspA1I CMGCKG 3 cut(s) 443, 1212, 1493
MspCI CTTAAG 2 cut(s) 1198, 1454
MspI CCGG 5 cut(s) 910, 973, 1715, 1778, 1817
MspR9I CCNGG 1 cut(s) 893
Mva1269I GAATGC 2 cut(s) 515, 1680
MvaI CCWGG 1 cut(s) 893
MwoI GCNNNNNNNGC 4 cut(s) 165, 572, 1221, 1787
NcoI CCATGG 2 cut(s) 589, 947
NdeI CATATG 2 cut(s) 304, 820
NdeII GATC 8 cut(s) 60, 445, 475, 637, 709, 794, 1135, 1411
NlaIII CATG 9 cut(s) 99, 144, 221, 563, 593, 644, 951, 968, 1237
NlaIV GGNNCC 5 cut(s) 318, 623, 971, 1782, 1882
NmuCI GTSAC 6 cut(s) 146, 430, 929, 1089, 1449, 1458
NspV TTCGAA 1 cut(s) 1348
PagI TCATGA 1 cut(s) 1233
PaqCI CACCTGC 1 cut(s) 1550
PctI GAATGC 2 cut(s) 515, 1680
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 888, 1487
PkrI GCNGC 8 cut(s) 215, 274, 412, 1073, 1132, 1214, 1373, 1831
PleI GAGTC 3 cut(s) 860, 1456, 1620
PpsI GAGTC 3 cut(s) 860, 1456, 1620
Ppu21I YACGTR 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 891
PspFI CCCAGC 1 cut(s) 513
PspGI CCWGG 1 cut(s) 891
PspN4I GGNNCC 5 cut(s) 318, 623, 971, 1782, 1882
PspPI GGNCC 5 cut(s) 90, 317, 455, 970, 1814
PsuI RGATCY 1 cut(s) 1411
PvuII CAGCTG 3 cut(s) 443, 1212, 1493
RsaI GTAC 5 cut(s) 5, 50, 199, 623, 783
RsaNI GTAC 5 cut(s) 4, 49, 198, 622, 782
RseI CAYNNNNRTG 4 cut(s) 100, 281, 1544, 1761
SalI GTCGAC 1 cut(s) 534
SaqAI TTAA 5 cut(s) 900, 1199, 1433, 1455, 1566
SatI GCNGC 8 cut(s) 214, 273, 411, 1072, 1131, 1213, 1372, 1830
Sau3AI GATC 8 cut(s) 60, 445, 475, 637, 709, 794, 1135, 1411
Sau96I GGNCC 5 cut(s) 90, 317, 455, 970, 1814
SchI GAGTC 3 cut(s) 861, 1457, 1621
ScrFI CCNGG 1 cut(s) 893
SduI GDGCHC 4 cut(s) 109, 550, 598, 1264
SfaNI GCATC 5 cut(s) 261, 1117, 1429, 1624, 1798
SfcI CTRYAG 1 cut(s) 856
SfuI TTCGAA 1 cut(s) 1348
SinI GGWCC 4 cut(s) 90, 455, 970, 1814
SmiMI CAYNNNNRTG 4 cut(s) 100, 281, 1544, 1761
SmlI CTYRAG 2 cut(s) 1198, 1454
SmoI CTYRAG 2 cut(s) 1198, 1454
SnaBI TACGTA 1 cut(s) 281
Sse9I AATT 8 cut(s) 151, 256, 435, 861, 1205, 1519, 1533, 1675
SsiI CCGC 6 cut(s) 214, 250, 382, 841, 1224, 1301
SspI AATATT 1 cut(s) 1177
SspMI CTAG 2 cut(s) 978, 1839
StyD4I CCNGG 1 cut(s) 891
StyI CCWWGG 3 cut(s) 490, 589, 947
TaaI ACNGT 5 cut(s) 540, 582, 600, 1114, 1183
TaiI ACGT 1 cut(s) 283
TaqI TCGA 3 cut(s) 535, 1138, 1348
TasI AATT 8 cut(s) 151, 256, 435, 861, 1205, 1519, 1533, 1675
TatI WGTACW 1 cut(s) 48
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 2 cut(s) 888, 1487
Tru1I TTAA 5 cut(s) 900, 1199, 1433, 1455, 1566
Tru9I TTAA 5 cut(s) 900, 1199, 1433, 1455, 1566
TscAI CASTG 3 cut(s) 42, 545, 1546
TseFI GTSAC 6 cut(s) 146, 430, 929, 1089, 1449, 1458
TseI GCWGC 7 cut(s) 272, 410, 1071, 1130, 1212, 1371, 1829
Tsp45I GTSAC 6 cut(s) 146, 430, 929, 1089, 1449, 1458
TspDTI ATGAA 4 cut(s) 951, 975, 1482, 1755
TspRI CASTG 3 cut(s) 42, 545, 1546
Vha464I CTTAAG 2 cut(s) 1198, 1454
VneI GTGCAC 2 cut(s) 546, 594
VpaK11BI GGWCC 4 cut(s) 90, 455, 970, 1814
XagI CCTNNNNNAGG 1 cut(s) 831
XapI RAATTY 1 cut(s) 1205
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XmiI GTMKAC 2 cut(s) 330, 535
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 2 cut(s) 978, 1839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.