AT4G08590

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000644
Physical Location & Seq
Reverse (-)
5463794 .. 5466747
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08590.6

Sequence Viewer

Length: 1398 bp
ATGACGCGTGTCAACCAGCTTCCATGCGACTGTGTATCTACGGCGGAGGAATCTCTCACTTCCGGCACGTGCATTACCCCTACGCACGTGACTTCCCTTTCGTCTCCTCTTGATCGCTCCGGCGATGTTGATCCTCTTCCTGTTTCCGACGAATCTGGTGGCTCGAAGGCCGATGAATCTATGACTGACGCAGATGAGACTAAAAAGAGGAAACGGATACTCAGTGGTGATTGTGAAGCCGATGAGAATAATAAGAGTGACGGAGAAATCGCGAGTCTCAATGACGGTGTTGATGCGTTTACTGCGATTTGTGAAGACTTGAATTGTTCTCTGTGTAATCAATTGCCTGATAGGCCCGTCACGATATTATGTGGACATAACTTTTGCTTGAAATGTTTTGACAAGTGGATTGATCAAGGGAATCAAATTTGTGCTACATGTCGTAGCACAATTCCTGACAAAATGGCTGCCAATCCTCGTGTTAACTCGTCTCTTGTGTCTGTTATCCGTTATGTAAAAGTTGCTAAAACTGCTGGTGTTGGTACTGCAAATTTTTTTCCTTTTACAAGCAACCAAGACGGCCCAGAGAATGCCTTTAGAACCAAGCGCGCTAAAATCGGGGAGGAGAATGCTGCAAGGATATATGTTACCGTACCATTTGATCACTTTGGTCCTATACCAGCTGAACATGATCCTGTCAGGAACCAAGGTGTTTTAGTTGGAGAATCCTGGGAGAATCGAGTAGAGTGTCGGCAGTGGGGGGTTCACTTGCCACATGTTTCTTGCATTGCTGGACAAGAAGACTATGGAGCTCAGTCTGTAGTAATATCTGGAGGTTATAAGGATGACGAGGATCATGGAGAATGGTTTCTATACACAGGAAGGAGTAGAGGGAGACATTTCGCTAACGAGGATCAGGAGTTTGAAGACTTGAATGAAGCTTTAAGAGTTAGTTGCGAAATGGGGTATCCAGTTCGAGTTGTCAGGTCTTACAAGGATAGGTATTCTGCATATGCCCCTAAGGAAGGAGTGAGATATGATGGTGTATACAGGATCGAGAAATGCTGGCGAAAAGCTAGATTCCCGGATTCATTTAAGGTCTGTCGTTACCTGTTTGTAAGATGTGACAATGAGCCAGCTCCATGGAACAGTGATGAGAGTGGAGATCGTCCAAGACCTTTGCCTAATATTCCAGAGCTTGAAACGGCCTCAGACCTGTTTGAGAGAAAGGAAAGTCCATCATGGGATTTTGATGAAGCCGAGGGCCGTTGGAGATGGATGAAGCCTCCACCTGCAAATCATGAGCAGAGGGAGAGAATGAAGATGGCTATGACATGTCTTCTCCTTTTTGTCCTTATCATTCTCGTTGGTTCATCATCTATCTTATATCAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

465

Amino Acids

52.31

Weight (kDa)

5.12

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 106 - 151 2.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 108 - 148 7.3e-07 Ring finger domain
zf-C3HC4 PF00097 109 - 147 1.4e-07 Zinc finger, C3HC4 type (RING finger)
SAD_SRA PF02182 235 - 377 3.7e-43 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AarI CACCTGC 1 cut(s) 1300
Acc36I ACCTGC 1 cut(s) 1300
AccI GTMKAC 1 cut(s) 1047
AccII CGCG 3 cut(s) 7, 272, 609
AciI CCGC 1 cut(s) 44
AclWI GGATC 5 cut(s) 125, 686, 861, 921, 1061
AcsI RAATTY 2 cut(s) 426, 550
AcvI CACGTG 2 cut(s) 69, 88
AfaI GTAC 2 cut(s) 544, 654
AfiI CCNNNNNNNGG 1 cut(s) 1025
AflIII ACRYGT 4 cut(s) 5, 437, 775, 1334
AgsI TTSAA 5 cut(s) 322, 391, 926, 934, 1202
AjnI CCWGG 1 cut(s) 728
AluBI AGCT 7 cut(s) 19, 683, 812, 941, 1076, 1139, 1198
AluI AGCT 7 cut(s) 19, 683, 812, 941, 1076, 1139, 1198
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 5 cut(s) 108, 191, 281, 495, 889
AlwI GGATC 5 cut(s) 125, 686, 861, 921, 1061
AoxI GGCC 5 cut(s) 168, 353, 580, 1206, 1264
ApeKI GCWGC 2 cut(s) 467, 632
ApoI RAATTY 2 cut(s) 426, 550
ArsI GACNNNNNNTTYG 4 cut(s) 82, 114, 366, 398
Asp700I GAANNNNTTC 1 cut(s) 867
AspLEI GCGC 2 cut(s) 609, 611
AspS9I GGNCC 4 cut(s) 354, 581, 671, 1264
AsuC2I CCSGG 1 cut(s) 1085
AsuHPI GGTGA 1 cut(s) 239
AvaII GGWCC 1 cut(s) 671
AxyI CCTNAGG 1 cut(s) 1020
BanII GRGCYC 1 cut(s) 814
BauI CACGAG 1 cut(s) 477
BbrPI CACGTG 2 cut(s) 69, 88
BbsI GAAGAC 4 cut(s) 321, 807, 933, 1331
Bbv12I GWGCWC 1 cut(s) 814
BbvI GCAGC 2 cut(s) 454, 619
BccI CCATC 4 cut(s) 1034, 1246, 1269, 1318
BceAI ACGGC 4 cut(s) 57, 595, 1221, 1251
BciT130I CCWGG 1 cut(s) 730
BciVI GTATCC 2 cut(s) 210, 978
BclI TGATCA 2 cut(s) 412, 661
BcnI CCSGG 1 cut(s) 1085
BcoDI GTCTC 5 cut(s) 108, 191, 281, 495, 889
BfaI CTAG 1 cut(s) 1077
BfmI CTRYAG 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 1300
BfuI GTATCC 2 cut(s) 210, 978
BglI GCCNNNNNGGC 1 cut(s) 352
BisI GCNGC 2 cut(s) 468, 633
BlsI GCNGC 2 cut(s) 469, 634
Bme1390I CCNGG 2 cut(s) 730, 1085
Bme18I GGWCC 1 cut(s) 671
BmgT120I GGNCC 4 cut(s) 354, 581, 671, 1264
BmiI GGNNCC 1 cut(s) 704
BmrFI CCNGG 2 cut(s) 730, 1085
BmsI GCATC 1 cut(s) 283
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 4 cut(s) 321, 807, 933, 1331
BpmI CTGGAG 1 cut(s) 852
BpuMI CCSGG 1 cut(s) 1085
BsaAI YACGTR 2 cut(s) 69, 88
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 4 cut(s) 706, 729, 1142, 1260
Bsc4I CCNNNNNNNGG 1 cut(s) 1025
Bse1I ACTGG 1 cut(s) 971
Bse21I CCTNAGG 1 cut(s) 1020
Bse3DI GCAATG 1 cut(s) 786
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 1 cut(s) 730
BseDI CCNNGG 4 cut(s) 706, 729, 1142, 1260
BseGI GGATG 2 cut(s) 850, 1284
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 1025
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 3 cut(s) 235, 827, 1224
BseNI ACTGG 1 cut(s) 971
BsePI GCGCGC 1 cut(s) 607
BseRI GAGGAG 2 cut(s) 96, 638
BseXI GCAGC 2 cut(s) 454, 619
Bsh1236I CGCG 3 cut(s) 7, 272, 609
BshFI GGCC 5 cut(s) 170, 355, 582, 1208, 1266
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 3 cut(s) 63, 120, 1085
BslI CCNNNNNNNGG 1 cut(s) 1025
BsmAI GTCTC 5 cut(s) 108, 191, 281, 495, 889
BsmBI CGTCTC 2 cut(s) 108, 495
BsmI GAATGC 2 cut(s) 595, 634
BsnI GGCC 5 cut(s) 170, 355, 582, 1208, 1266
Bsp1286I GDGCHC 1 cut(s) 814
Bsp143I GATC 9 cut(s) 112, 130, 412, 661, 691, 853, 913, 1053, 1165
Bsp19I CCATGG 1 cut(s) 1142
Bsp68I TCGCGA 1 cut(s) 272
BspACI CCGC 1 cut(s) 44
BspANI GGCC 5 cut(s) 170, 355, 582, 1208, 1266
BspCNI CTCAG 3 cut(s) 234, 826, 1223
BspFNI CGCG 3 cut(s) 7, 272, 609
BspHI TCATGA 1 cut(s) 1300
BspLI GGNNCC 1 cut(s) 704
BspMI ACCTGC 1 cut(s) 1300
BspPI GGATC 5 cut(s) 125, 686, 861, 921, 1061
BsrDI GCAATG 1 cut(s) 786
BsrI ACTGG 1 cut(s) 971
BssECI CCNNGG 4 cut(s) 706, 729, 1142, 1260
BssHII GCGCGC 1 cut(s) 607
BssMI GATC 9 cut(s) 112, 130, 412, 661, 691, 853, 913, 1053, 1165
BssNAI GTATAC 1 cut(s) 1048
BssSI CACGAG 1 cut(s) 477
BssT1I CCWWGG 2 cut(s) 706, 1142
Bst1107I GTATAC 1 cut(s) 1048
Bst2BI CACGAG 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 730
Bst4CI ACNGT 4 cut(s) 32, 287, 652, 1151
Bst6I CTCTTC 1 cut(s) 141
BstBAI YACGTR 2 cut(s) 69, 88
BstC8I GCNNGC 3 cut(s) 609, 1067, 1137
BstDEI CTNAG 4 cut(s) 221, 813, 1020, 1210
BstDSI CCRYGG 1 cut(s) 1142
BstENI CCTNNNNNAGG 1 cut(s) 1023
BstF5I GGATG 2 cut(s) 850, 1284
BstFNI CGCG 3 cut(s) 7, 272, 609
BstHHI GCGC 2 cut(s) 609, 611
BstKTI GATC 9 cut(s) 115, 133, 415, 664, 694, 856, 916, 1056, 1168
BstMAI GTCTC 5 cut(s) 108, 191, 281, 495, 889
BstMBI GATC 9 cut(s) 112, 130, 412, 661, 691, 853, 913, 1053, 1165
BstMWI GCNNNNNNNGC 3 cut(s) 302, 352, 530
BstNI CCWGG 1 cut(s) 730
BstNSI RCATGY 3 cut(s) 441, 779, 1338
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 728, 1083
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 3 cut(s) 7, 272, 609
BstV1I GCAGC 2 cut(s) 454, 619
BstV2I GAAGAC 4 cut(s) 321, 807, 933, 1331
BstXI CCANNNNNNTGG 1 cut(s) 1143
BstZ17I GTATAC 1 cut(s) 1048
Bsu36I CCTNAGG 1 cut(s) 1020
BsuI GTATCC 2 cut(s) 210, 978
BsuRI GGCC 5 cut(s) 170, 355, 582, 1208, 1266
BtgI CCRYGG 1 cut(s) 1142
BtgZI GCGATG 1 cut(s) 138
BtsCI GGATG 2 cut(s) 850, 1284
BtsI GCAGTG 1 cut(s) 761
BtsIMutI CAGTG 3 cut(s) 229, 761, 1156
BtuMI TCGCGA 1 cut(s) 272
BveI ACCTGC 1 cut(s) 1300
Cac8I GCNNGC 3 cut(s) 609, 1067, 1137
CciI TCATGA 1 cut(s) 1300
CfoI GCGC 2 cut(s) 609, 611
Cfr13I GGNCC 4 cut(s) 354, 581, 671, 1264
CseI GACGC 2 cut(s) 13, 197
Csp6I GTAC 2 cut(s) 543, 653
CviAII CATG 9 cut(s) 24, 438, 689, 776, 857, 1143, 1242, 1301, 1335
CviQI GTAC 2 cut(s) 543, 653
DdeI CTNAG 4 cut(s) 221, 813, 1020, 1210
DpnI GATC 9 cut(s) 114, 132, 414, 663, 693, 855, 915, 1055, 1167
DpnII GATC 9 cut(s) 112, 130, 412, 661, 691, 853, 913, 1053, 1165
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 812
Eco130I CCWWGG 2 cut(s) 706, 1142
Eco24I GRGCYC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 671
Eco53kI GAGCTC 1 cut(s) 812
Eco72I CACGTG 2 cut(s) 69, 88
Eco81I CCTNAGG 1 cut(s) 1020
EcoICRI GAGCTC 1 cut(s) 812
EcoNI CCTNNNNNAGG 1 cut(s) 1023
EcoRII CCWGG 1 cut(s) 728
EcoT14I CCWWGG 2 cut(s) 706, 1142
EcoT38I GRGCYC 1 cut(s) 814
ErhI CCWWGG 2 cut(s) 706, 1142
Esp3I CGTCTC 2 cut(s) 108, 495
FaeI CATG 9 cut(s) 27, 441, 692, 779, 860, 1146, 1245, 1304, 1338
FatI CATG 9 cut(s) 23, 437, 688, 775, 856, 1142, 1241, 1300, 1334
FauNDI CATATG 1 cut(s) 1012
FbaI TGATCA 2 cut(s) 412, 661
FblI GTMKAC 1 cut(s) 1047
Fnu4HI GCNGC 2 cut(s) 468, 633
FokI GGATG 2 cut(s) 857, 1291
FriOI GRGCYC 1 cut(s) 814
Fsp4HI GCNGC 2 cut(s) 468, 633
FspBI CTAG 1 cut(s) 1077
GlaI GCGC 2 cut(s) 608, 610
GluI GCNGC 2 cut(s) 468, 633
GsuI CTGGAG 1 cut(s) 852
HaeIII GGCC 5 cut(s) 170, 355, 582, 1208, 1266
HapII CCGG 3 cut(s) 63, 120, 1085
HgaI GACGC 2 cut(s) 13, 197
HhaI GCGC 2 cut(s) 609, 611
Hin1II CATG 9 cut(s) 27, 441, 692, 779, 860, 1146, 1245, 1304, 1338
Hin6I GCGC 2 cut(s) 607, 609
HinP1I GCGC 2 cut(s) 607, 609
HincII GTYRAC 2 cut(s) 13, 484
HindII GTYRAC 2 cut(s) 13, 484
HindIII AAGCTT 1 cut(s) 939
HinfI GANTC 9 cut(s) 50, 152, 176, 274, 421, 725, 736, 1080, 1088
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 3 cut(s) 63, 120, 1085
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 6 cut(s) 13, 300, 374, 484, 766, 1048
Hpy188I TCNGA 2 cut(s) 148, 1213
Hpy8I GTNNAC 6 cut(s) 13, 300, 374, 484, 766, 1048
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 3 cut(s) 160, 876, 1019
HpyCH4III ACNGT 4 cut(s) 32, 287, 652, 1151
HpyCH4IV ACGT 2 cut(s) 68, 87
HpyCH4V TGCA 6 cut(s) 72, 548, 635, 786, 1010, 1295
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 352, 530
HpyF3I CTNAG 4 cut(s) 221, 813, 1020, 1210
HpySE526I ACGT 2 cut(s) 68, 87
Hsp92II CATG 9 cut(s) 27, 441, 692, 779, 860, 1146, 1245, 1304, 1338
HspAI GCGC 2 cut(s) 607, 609
Ksp22I TGATCA 2 cut(s) 412, 661
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 9 cut(s) 112, 130, 412, 661, 691, 853, 913, 1053, 1165
LmnI GCTCC 3 cut(s) 122, 809, 1144
Lsp1109I GCAGC 2 cut(s) 454, 619
LweI GCATC 1 cut(s) 283
MaeI CTAG 1 cut(s) 1077
MaeII ACGT 2 cut(s) 68, 87
MaeIII GTNAC 6 cut(s) 88, 257, 358, 646, 1106, 1124
MalI GATC 9 cut(s) 114, 132, 414, 663, 693, 855, 915, 1055, 1167
MboI GATC 9 cut(s) 112, 130, 412, 661, 691, 853, 913, 1053, 1165
MboII GAAGA 6 cut(s) 128, 326, 812, 938, 1331, 1333
MfeI CAATTG 1 cut(s) 341
MhlI GDGCHC 1 cut(s) 814
MluCI AATT 5 cut(s) 322, 341, 426, 450, 550
MluI ACGCGT 1 cut(s) 5
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 3 cut(s) 171, 700, 1250
MroXI GAANNNNTTC 1 cut(s) 867
MseI TTAA 3 cut(s) 483, 944, 1095
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 3 cut(s) 63, 120, 1085
MspR9I CCNGG 2 cut(s) 730, 1085
MunI CAATTG 1 cut(s) 341
Mva1269I GAATGC 2 cut(s) 595, 634
MvaI CCWGG 1 cut(s) 730
MvnI CGCG 3 cut(s) 7, 272, 609
MwoI GCNNNNNNNGC 3 cut(s) 302, 352, 530
NciI CCSGG 1 cut(s) 1085
NcoI CCATGG 1 cut(s) 1142
NdeI CATATG 1 cut(s) 1012
NdeII GATC 9 cut(s) 112, 130, 412, 661, 691, 853, 913, 1053, 1165
NlaIII CATG 9 cut(s) 27, 441, 692, 779, 860, 1146, 1245, 1304, 1338
NlaIV GGNNCC 1 cut(s) 704
NmeAIII GCCGAG 1 cut(s) 1285
NmuCI GTSAC 4 cut(s) 88, 257, 358, 1124
NruI TCGCGA 1 cut(s) 272
NspI RCATGY 3 cut(s) 441, 779, 1338
PagI TCATGA 1 cut(s) 1300
PaqCI CACCTGC 1 cut(s) 1300
PauI GCGCGC 1 cut(s) 607
PciI ACATGT 3 cut(s) 437, 775, 1334
PctI GAATGC 2 cut(s) 595, 634
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 8 cut(s) 50, 152, 176, 421, 725, 736, 1080, 1088
PfoI TCCNGGA 1 cut(s) 1083
PkrI GCNGC 2 cut(s) 469, 634
PleI GAGTC 1 cut(s) 282
PmaCI CACGTG 2 cut(s) 69, 88
PmlI CACGTG 2 cut(s) 69, 88
PpsI GAGTC 1 cut(s) 282
Ppu21I YACGTR 2 cut(s) 69, 88
PscI ACATGT 3 cut(s) 437, 775, 1334
PshAI GACNNNNGTC 1 cut(s) 8
PsiI TTATAA 1 cut(s) 840
Psp124BI GAGCTC 1 cut(s) 814
Psp6I CCWGG 1 cut(s) 728
PspCI CACGTG 2 cut(s) 69, 88
PspGI CCWGG 1 cut(s) 728
PspN4I GGNNCC 1 cut(s) 704
PspPI GGNCC 4 cut(s) 354, 581, 671, 1264
PteI GCGCGC 1 cut(s) 607
PvuII CAGCTG 1 cut(s) 683
RruI TCGCGA 1 cut(s) 272
RsaI GTAC 2 cut(s) 544, 654
RsaNI GTAC 2 cut(s) 543, 653
SacI GAGCTC 1 cut(s) 814
SaqAI TTAA 3 cut(s) 483, 944, 1095
SatI GCNGC 2 cut(s) 468, 633
Sau3AI GATC 9 cut(s) 112, 130, 412, 661, 691, 853, 913, 1053, 1165
Sau96I GGNCC 4 cut(s) 354, 581, 671, 1264
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 2 cut(s) 730, 1085
SduI GDGCHC 1 cut(s) 814
SfaNI GCATC 1 cut(s) 283
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 1 cut(s) 671
Sse9I AATT 5 cut(s) 322, 341, 426, 450, 550
SsiI CCGC 1 cut(s) 44
SspI AATATT 1 cut(s) 1189
SspMI CTAG 1 cut(s) 1077
SstI GAGCTC 1 cut(s) 814
StyD4I CCNGG 2 cut(s) 728, 1083
StyI CCWWGG 2 cut(s) 706, 1142
TaaI ACNGT 4 cut(s) 32, 287, 652, 1151
TaiI ACGT 2 cut(s) 71, 90
TaqI TCGA 4 cut(s) 164, 739, 976, 1056
TasI AATT 5 cut(s) 322, 341, 426, 450, 550
TfiI GAWTC 8 cut(s) 50, 152, 176, 421, 725, 736, 1080, 1088
Tru1I TTAA 3 cut(s) 483, 944, 1095
Tru9I TTAA 3 cut(s) 483, 944, 1095
TscAI CASTG 3 cut(s) 229, 761, 1156
TseFI GTSAC 4 cut(s) 88, 257, 358, 1124
TseI GCWGC 2 cut(s) 467, 632
Tsp45I GTSAC 4 cut(s) 88, 257, 358, 1124
TspDTI ATGAA 7 cut(s) 189, 951, 1080, 1269, 1295, 1334, 1362
TspGWI ACGGA 3 cut(s) 229, 276, 497
TspRI CASTG 3 cut(s) 229, 761, 1156
VpaK11BI GGWCC 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 1023
XapI RAATTY 2 cut(s) 426, 550
XceI RCATGY 3 cut(s) 441, 779, 1338
XmiI GTMKAC 1 cut(s) 1047
XmnI GAANNNNTTC 1 cut(s) 867
XspI CTAG 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.