Rroxscaffold_7G00212960

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000644
Physical Location & Seq
Forward (+)
63494250 .. 63505618
11369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212960.1

Sequence Viewer

Length: 888 bp
ATGTGCAAATTGCCGCCAATCGATCCCCTCCACTCGAGAATCAACTCTAATCTTGTGGGAAGCATTTGTATTGCCAAGGCATCACAACGCGGTGGCTCGGGGGAGTACCTACCTCGGTTGGCTTATCGCATTCGAAACCGGGACCGTCTGGACAAAGCATTTACAACCGAGCGAGCCAAAAAGCACGGGAAAGCTAATGCCTCAAGTGGAAGGATCTTTGTGACATCAACTCCGGATCATTTTGGGCCCATAACCGCCGAATATGATCCTAAGAAGAATAAGGGTGTGGCGGTGGGGGAGACTTGGGGGCATAGGATTGAATGTAGACAATGGGGTATTCATCGCCCTATGGTTTCGGGATTTACGGGGCGGTGCAAGTATGGCGCCCAATCGGTGGTTCTCTCGGGAGGGTATATAGACGATGAAGATCACGGTGAATGGTTCATTTACACCGGGAGGTATCAGAATCATCGTCTCAAAACTAAGGCCTCTCGTTTGATTAGCAACTCAAGGGAAGTTCAACAAGCCAAAGAACTCGAGTTTTGGTGGCCGGTGCTAGGAGAAAACTCGGTTGGAACCCAATTCGGCCGAAAATGGAAAAGCGATTCCCGCCGCCGATCCGGCGATTCCGAGGCGCAAGGCTGCCACCGGCAGCTGCGCAAGGTTGAGCGAAGCCGTGGGAAGTGTTTGATTAGCAACTCAAGGGAAGTTCAACAAGCCAAAGAACTCGAGTTTTGGTGGTCGGAGCTAGGAGAAAACTCGGTTGGAACCCAATTCGGCCGAAAATGGAAAAGCGATTCCCGCCGCCGATACGGCGATTCCGAGGCGCAAGGTCGCCACGGCGGCTGCGCAAGGTTGAGGCAAAGGCGTGGGAAGTGGTCGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

295

Amino Acids

33.71

Weight (kDa)

10.15

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 93 - 163 3.5e-19 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 659, 850
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 394
AccI GTMKAC 1 cut(s) 325
AccII CGCG 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 232
AclWI GGATC 5 cut(s) 17, 221, 243, 260, 612
AcoI YGGCCR 3 cut(s) 548, 586, 778
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 394, 557
AgsI TTSAA 3 cut(s) 320, 521, 713
AjuI GAANNNNNNNTTGG 6 cut(s) 381, 413, 555, 587, 747, 779
AluBI AGCT 3 cut(s) 194, 655, 748
AluI AGCT 3 cut(s) 194, 655, 748
Alw26I GTCTC 2 cut(s) 293, 479
AlwI GGATC 5 cut(s) 17, 221, 243, 260, 612
Ama87I CYCGRG 5 cut(s) 34, 97, 403, 536, 728
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 5 cut(s) 245, 486, 548, 586, 778
ApaI GGGCCC 1 cut(s) 249
ApeKI GCWGC 4 cut(s) 642, 652, 655, 846
AspLEI GCGC 5 cut(s) 386, 637, 660, 829, 851
AspS9I GGNCC 3 cut(s) 142, 245, 246
AsuC2I CCSGG 2 cut(s) 140, 454
AsuHPI GGTGA 1 cut(s) 446
AsuII TTCGAA 1 cut(s) 133
AvaI CYCGRG 5 cut(s) 34, 97, 403, 536, 728
AvaII GGWCC 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 249
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 249
BbvI GCAGC 4 cut(s) 629, 642, 664, 833
BceAI ACGGC 3 cut(s) 660, 829, 856
BcnI CCSGG 2 cut(s) 140, 454
BcoDI GTCTC 2 cut(s) 293, 479
BfaI CTAG 2 cut(s) 557, 749
BfoI RGCGCY 1 cut(s) 387
BglI GCCNNNNNGGC 3 cut(s) 621, 813, 843
BisI GCNGC 8 cut(s) 14, 613, 643, 653, 656, 805, 844, 847
BlsI GCNGC 8 cut(s) 15, 614, 644, 654, 657, 806, 845, 848
Bme1390I CCNGG 2 cut(s) 140, 454
Bme18I GGWCC 1 cut(s) 142
BmeT110I CYCGRG 5 cut(s) 34, 97, 403, 536, 728
BmgT120I GGNCC 3 cut(s) 142, 245, 246
BmiI GGNNCC 5 cut(s) 143, 247, 385, 577, 769
BmrFI CCNGG 2 cut(s) 140, 454
BmsI GCATC 1 cut(s) 89
Bpu14I TTCGAA 1 cut(s) 133
BpuEI CTTGAG 3 cut(s) 187, 493, 685
BpuMI CCSGG 2 cut(s) 140, 454
Bsa29I ATCGAT 1 cut(s) 21
BsaBI GATNNNNATC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 384
BsaJI CCNNGG 6 cut(s) 75, 113, 630, 676, 822, 838
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 214, 244
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 557
Bse118I RCCGGY 2 cut(s) 550, 648
Bse8I GATNNNNATC 1 cut(s) 426
BseAI TCCGGA 1 cut(s) 232
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 6 cut(s) 75, 113, 630, 676, 822, 838
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 2 cut(s) 394, 557
BseSI GKGCMC 1 cut(s) 249
BseX3I CGGCCG 2 cut(s) 586, 778
BseXI GCAGC 4 cut(s) 629, 642, 664, 833
Bsh1236I CGCG 1 cut(s) 90
Bsh1285I CGRYCG 2 cut(s) 589, 781
BshFI GGCC 5 cut(s) 247, 488, 550, 588, 780
BshNI GGYRCC 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 21
BsiEI CGRYCG 2 cut(s) 589, 781
BsiHKCI CYCGRG 5 cut(s) 34, 97, 403, 536, 728
BsiSI CCGG 6 cut(s) 139, 233, 453, 551, 621, 649
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 2 cut(s) 394, 557
BsmAI GTCTC 2 cut(s) 293, 479
BsmBI CGTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 5 cut(s) 247, 488, 550, 588, 780
BsoBI CYCGRG 5 cut(s) 34, 97, 403, 536, 728
Bsp119I TTCGAA 1 cut(s) 133
Bsp120I GGGCCC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 249
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 6 cut(s) 22, 213, 235, 265, 427, 617
BspANI GGCC 5 cut(s) 247, 488, 550, 588, 780
BspDI ATCGAT 1 cut(s) 21
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 5 cut(s) 143, 247, 385, 577, 769
BspPI GGATC 5 cut(s) 17, 221, 243, 260, 612
BspT104I TTCGAA 1 cut(s) 133
BspT107I GGYRCC 1 cut(s) 383
BsrFI RCCGGY 2 cut(s) 550, 648
BssAI RCCGGY 2 cut(s) 550, 648
BssECI CCNNGG 6 cut(s) 75, 113, 630, 676, 822, 838
BssMI GATC 6 cut(s) 22, 213, 235, 265, 427, 617
BssNI GRCGYC 1 cut(s) 384
BssT1I CCWWGG 1 cut(s) 75
Bst4CI ACNGT 2 cut(s) 146, 434
BstACI GRCGYC 1 cut(s) 384
BstBI TTCGAA 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 2 cut(s) 270, 483
BstDSI CCRYGG 2 cut(s) 676, 838
BstFNI CGCG 1 cut(s) 90
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 5 cut(s) 386, 637, 660, 829, 851
BstKTI GATC 6 cut(s) 25, 216, 238, 268, 430, 620
BstMAI GTCTC 2 cut(s) 293, 479
BstMBI GATC 6 cut(s) 22, 213, 235, 265, 427, 617
BstMCI CGRYCG 2 cut(s) 589, 781
BstMWI GCNNNNNNNGC 6 cut(s) 381, 609, 621, 801, 813, 843
BstSCI CCNGG 2 cut(s) 138, 452
BstSLI GKGCMC 1 cut(s) 249
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 4 cut(s) 629, 642, 664, 833
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BstZI CGGCCG 2 cut(s) 586, 778
Bsu15I ATCGAT 1 cut(s) 21
BsuRI GGCC 5 cut(s) 247, 488, 550, 588, 780
BsuTUI ATCGAT 1 cut(s) 21
BtgI CCRYGG 2 cut(s) 676, 838
BtgZI GCGATG 1 cut(s) 326
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 5 cut(s) 386, 637, 660, 829, 851
Cfr10I RCCGGY 2 cut(s) 550, 648
Cfr13I GGNCC 3 cut(s) 142, 245, 246
ClaI ATCGAT 1 cut(s) 21
Csp6I GTAC 1 cut(s) 106
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 2 cut(s) 270, 483
DinI GGCGCC 1 cut(s) 385
DpnI GATC 6 cut(s) 24, 215, 237, 267, 429, 619
DpnII GATC 6 cut(s) 22, 213, 235, 265, 427, 617
EaeI YGGCCR 3 cut(s) 548, 586, 778
EagI CGGCCG 2 cut(s) 586, 778
EclXI CGGCCG 2 cut(s) 586, 778
Eco130I CCWWGG 1 cut(s) 75
Eco147I AGGCCT 1 cut(s) 488
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 142
Eco52I CGGCCG 2 cut(s) 586, 778
Eco88I CYCGRG 5 cut(s) 34, 97, 403, 536, 728
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 479
FaiI YATR 7 cut(s) 251, 264, 312, 350, 381, 414, 416
FaqI GGGAC 1 cut(s) 155
FauI CCCGC 2 cut(s) 617, 809
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 8 cut(s) 14, 613, 643, 653, 656, 805, 844, 847
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 8 cut(s) 14, 613, 643, 653, 656, 805, 844, 847
FspBI CTAG 2 cut(s) 557, 749
FspI TGCGCA 2 cut(s) 659, 850
GlaI GCGC 5 cut(s) 385, 636, 659, 828, 850
GluI GCNGC 8 cut(s) 14, 613, 643, 653, 656, 805, 844, 847
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 5 cut(s) 247, 488, 550, 588, 780
HapII CCGG 6 cut(s) 139, 233, 453, 551, 621, 649
HhaI GCGC 5 cut(s) 386, 637, 660, 829, 851
Hin1I GRCGYC 1 cut(s) 384
Hin6I GCGC 5 cut(s) 384, 635, 658, 827, 849
HinP1I GCGC 5 cut(s) 384, 635, 658, 827, 849
HinfI GANTC 6 cut(s) 39, 466, 605, 626, 797, 818
HpaII CCGG 6 cut(s) 139, 233, 453, 551, 621, 649
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 4 cut(s) 465, 631, 745, 823
Hpy188III TCNNGA 5 cut(s) 36, 149, 233, 357, 405
Hpy8I GTNNAC 1 cut(s) 326
HpyAV CCTTC 1 cut(s) 204
HpyCH4III ACNGT 2 cut(s) 146, 434
HpyCH4V TGCA 2 cut(s) 6, 375
HpyF10VI GCNNNNNNNGC 6 cut(s) 381, 609, 621, 801, 813, 843
HpyF3I CTNAG 2 cut(s) 270, 483
Hsp92I GRCGYC 1 cut(s) 384
HspAI GCGC 5 cut(s) 384, 635, 658, 827, 849
KasI GGCGCC 1 cut(s) 383
Kpn2I TCCGGA 1 cut(s) 232
Kzo9I GATC 6 cut(s) 22, 213, 235, 265, 427, 617
LmnI GCTCC 1 cut(s) 745
LpnPI CCDG 7 cut(s) 134, 152, 246, 466, 564, 634, 662
Lsp1109I GCAGC 4 cut(s) 629, 642, 664, 833
LweI GCATC 1 cut(s) 89
MaeI CTAG 2 cut(s) 557, 749
MaeIII GTNAC 1 cut(s) 220
MalI GATC 6 cut(s) 24, 215, 237, 267, 429, 619
MboI GATC 6 cut(s) 22, 213, 235, 265, 427, 617
MboII GAAGA 2 cut(s) 286, 437
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 3 cut(s) 8, 581, 773
Mly113I GGCGCC 1 cut(s) 384
MmeI TCCRAC 3 cut(s) 553, 723, 745
MnlI CCTC 9 cut(s) 38, 123, 211, 401, 450, 499, 625, 817, 852
MroI TCCGGA 1 cut(s) 232
MspA1I CMGCKG 1 cut(s) 655
MspI CCGG 6 cut(s) 139, 233, 453, 551, 621, 649
MspR9I CCNGG 2 cut(s) 140, 454
Mva1269I GAATGC 1 cut(s) 129
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 6 cut(s) 381, 609, 621, 801, 813, 843
NarI GGCGCC 1 cut(s) 384
NciI CCSGG 2 cut(s) 140, 454
NdeII GATC 6 cut(s) 22, 213, 235, 265, 427, 617
NlaIV GGNNCC 5 cut(s) 143, 247, 385, 577, 769
NmuCI GTSAC 1 cut(s) 220
NsbI TGCGCA 2 cut(s) 659, 850
NspV TTCGAA 1 cut(s) 133
PaeR7I CTCGAG 3 cut(s) 34, 536, 728
PceI AGGCCT 1 cut(s) 488
PcsI WCGNNNNNNNCGW 1 cut(s) 819
PctI GAATGC 1 cut(s) 129
PfeI GAWTC 6 cut(s) 39, 466, 605, 626, 797, 818
PflMI CCANNNNNTGG 1 cut(s) 394
PkrI GCNGC 8 cut(s) 15, 614, 644, 654, 657, 806, 845, 848
PluTI GGCGCC 1 cut(s) 387
PspN4I GGNNCC 5 cut(s) 143, 247, 385, 577, 769
PspOMI GGGCCC 1 cut(s) 245
PspPI GGNCC 3 cut(s) 142, 245, 246
PspXI VCTCGAGB 2 cut(s) 536, 728
PsuI RGATCY 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 655
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SatI GCNGC 8 cut(s) 14, 613, 643, 653, 656, 805, 844, 847
Sau3AI GATC 6 cut(s) 22, 213, 235, 265, 427, 617
Sau96I GGNCC 3 cut(s) 142, 245, 246
ScrFI CCNGG 2 cut(s) 140, 454
SduI GDGCHC 1 cut(s) 249
SetI ASST 9 cut(s) 111, 115, 196, 461, 657, 666, 750, 835, 857
SfaNI GCATC 1 cut(s) 89
SfoI GGCGCC 1 cut(s) 385
Sfr274I CTCGAG 3 cut(s) 34, 536, 728
SfuI TTCGAA 1 cut(s) 133
SinI GGWCC 1 cut(s) 142
SlaI CTCGAG 3 cut(s) 34, 536, 728
SmlI CTYRAG 6 cut(s) 34, 202, 508, 536, 700, 728
SmoI CTYRAG 6 cut(s) 34, 202, 508, 536, 700, 728
Sse9I AATT 3 cut(s) 8, 581, 773
SseBI AGGCCT 1 cut(s) 488
SspDI GGCGCC 1 cut(s) 383
SspMI CTAG 2 cut(s) 557, 749
StuI AGGCCT 1 cut(s) 488
StyD4I CCNGG 2 cut(s) 138, 452
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 146, 434
TaqI TCGA 5 cut(s) 21, 35, 133, 537, 729
TasI AATT 3 cut(s) 8, 581, 773
TauI GCSGC 4 cut(s) 16, 615, 807, 846
TfiI GAWTC 6 cut(s) 39, 466, 605, 626, 797, 818
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 4 cut(s) 642, 652, 655, 846
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 3 cut(s) 329, 433, 438
Van91I CCANNNNNTGG 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 142
XhoI CTCGAG 3 cut(s) 34, 536, 728
XmiI GTMKAC 1 cut(s) 325
XspI CTAG 2 cut(s) 557, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.