MD14G1146900.v1.1

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000644
Physical Location & Seq
Forward (+)
23950971 .. 23956466
5496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1146900.v1.1.491

Sequence Viewer

Length: 2568 bp
ATGGCGCAAGTTAGCCAGCTTCCCTGCGACGGAGACGGCGTCTGCATGCTCTGCAAGGCGACTCCGCCCGACGCCGAGAAGCTCACCTGCAAGACCTGCGTGACTCCCTGGCACGCTGCCTGCCTCTTGGCCAAGCCCGAGTCCCTGGCCGCCACTCTCCAGTGGGAGTGCCCCGACTGCACATCAATCTCCGGCGATGCTCCGGCGCACGTCCCCGCCGTGAACGCCGGCGTGGACGGCGGTTTGGTGGCGGCGATCCGGGAGATCGAGAACGACGCGACCCTGACGGAGCAGGAGAAGGCGAGGAGGAGGCAGGAGCTGATGAGCGGGAAGGCGACGGTGGAGGAGGAGAAGAAGGAGGCGAAGAACGACGTCTTGGATGTGCTTGGTGGGATTTTCAACTGCGTCTTCTGTATGCAGTTACCGGATAGGCCTGTTACGACACCATGTGGACACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGACAAGGGAAGCGTATTTGTGGAAAATGCCGTGGTCAAATTCCTCCCAAGATGGCAAGTAACCCCCGAATCAACTCTTCACTTGTATCTTGCATACGTACGGCTAAGTTGTCAAAAGCAAATGCAAACGGGGGGCCTGCTCGGGTTTATCATTATGTACAAAATCAAGACAGGCCGGACAAGGCGTTCACAACAGAACGTGCACAGAAGACTGGAAAAGCTAATGCTTCAAGTGGCAAGATATTTGTCACGATTCCAGGAGATCACTTTGGACCCATTCCTGCTGAAAATGATCCAGAGAGAAATCAAGGTGTTCTGGTTGGTGAGTGCTGGGAAGATAGGCTTGAATGTAGACAATGGGGTGCACATTTTCCCCATGTTGCTGGTATAGCTGGGCAGTCTGATCATGGTGCACAGTCAGTTGTGCTTTCAGGAGGCTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATATACCGGAAGTGGTGGAAGGGATTTGAGTGGTAATAAGCGAACAAACAAGAAACAGTCTTTTGACCAGACATTTGACAAATATAACAAGGCTCTCCGACTCAGTTGCCTGAAAGGTTATCCTGTGCGGGTGGTAAGGTCTCACAAGGAGAAGCGTTCCTCATATGCCCCTGCTGATAAAGGTGTTCGGTATGATGGGTGCTACAGAATAGAAAAATGTTGGCTTAAGGTTGGAGTCCAGGGGTTCAAAGTCTGCCGGTATCTTTTTGTCAGATGTGATAATGAACCTGCGCCATGGACGAGTGATGAGCATGGGGATCGACCTAGACCTTTACCTGATATTCCTGAGCTGAAGGCAGCAAAAGATATAACAGTGAGGAAAGGAAGTCCATCTTGGGACTTTGATGAAAAAGATGGCCGCTGGACATGGATGAAGCAACCACCTCCCAGCAAAAAACCTATTCTAGCATTCTGTCCTGAAGCTCAAAGGAAGACAACAAAAGCAATCAAGAAAGCACAAAAAATGACTGTACGACAGAAGCTCTTGAAAGAATTCAGCTGCCAAATTTGTCGGGAGGTGATGACTGCACCAGTAACCACACCTTGTGTTCATCACTTTTGCAAGTCGTGCTTAGAAGGTGCTTTTGTTGGCAAGAGTTTTGTGACAGAAAGAAGCAGAGGTGGACGGTCTCTCCGAGCCCAAAAGAATGTGATGAAATGCCCTGCCTGCCAGATGGATATCTCTGATTTTCTGAAGAATCCTCAGGTGAATACAGAGATAAAGGATATGATCGAGTCACTAAAAGCTCAGAATGAAAAGGAAGAGAAGGTGGATCCAACTGAGGAGTCAAGTGATGAAGAGGATGAGGATCCAAACGAGGTGTTAAGTGAAAATGATTCTGACAATGAAACTGCTGATCCTGTGTCTGACGAAGCAGGAGTGAATGGCAACGGGCAGACTTCACGAACAAAGTTGCCCACAAGGAGTCCCAAACGTCTGAAGGTTGATGCTGGTAATTCCTCATCAAAGACTGATAATGATGGGGAGTCAAAGGAAATTTTTGGTGCCCAGACTGATGGTTCGAAGGGAGATCCTAAAGAGAGCAAATCTGATGCTCCTGTTGCAAAGCGTGGGAGGAAACCTGCTGCTCAGGGTGGTGCTGGAGCGAAGGCCCGAGGAAGAAAGAGGACTAAGGAGGAGGAGGAAGCACCGGGTGATGCAGAAGGAAAATCTTCACCATCAAGTCCTCTGCAGGTGCAATCTGATGAGGATCCAAAAGAGAGCGAGAAGCCAGCTGCAAAATTTGGGAGGAAGCCTGCCAGTCAGGATGGTACTGGAGCAAAGACTAGAGGAAGAAAAAAGGCTCAGGAGAAAGAAGCATGGGGAAATGAAGAAGGAAATGGCTCACAGTCAAGTCCTCAGCAGGTGCAATCTGTTGAGGATCCAAAAGAGAACAAAAAACGAGCTGCAAAGCATGGCAGGAAGGCTGCTGGCGAGGGTGGTGCTGGAGCAAAGAAGGCTCGGGAGGAAGAAGCAATGGATGTTGATGATGGAAATGTTTCACCATCAAGCCCTCTGCACGTGAAAGCCGATGATTTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

856

Amino Acids

93.43

Weight (kDa)

8.37

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 134 - 177 3.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 134 - 174 2.3e-06 Ring finger domain
zf-C3HC4 PF00097 135 - 173 2.5e-06 Zinc finger, C3HC4 type (RING finger)
SAD_SRA PF02182 265 - 415 1.5e-48 SAD/SRA domain
zf-RING_UBOX PF13445 508 - 539 3.3e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 508 - 533 8.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 95, 2206, 2377
AatII GACGTC 1 cut(s) 375
Acc36I ACCTGC 6 cut(s) 95, 104, 1258, 2113, 2206, 2377
AccB1I GGYRCC 1 cut(s) 2027
AccBSI CCGCTC 1 cut(s) 327
AccI GTMKAC 1 cut(s) 841
AccII CGCG 1 cut(s) 278
AciI CCGC 8 cut(s) 65, 150, 216, 240, 251, 327, 1090, 1381
AcoI YGGCCR 3 cut(s) 129, 147, 1378
AcsI RAATTY 5 cut(s) 528, 1514, 1527, 2019, 2264
AcuI CTGAAG 5 cut(s) 488, 1334, 1461, 1736, 1982
AcvI CACGTG 1 cut(s) 2545
AcyI GRCGYC 3 cut(s) 39, 72, 372
AdeI CACNNNGTG 3 cut(s) 449, 1568, 2177
AfaI GTAC 4 cut(s) 589, 648, 1494, 2296
AfiI CCNNNNNNNGG 2 cut(s) 29, 484
AflII CTTAAG 1 cut(s) 1187
AgsI TTSAA 5 cut(s) 400, 720, 836, 1210, 1510
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 4 cut(s) 107, 144, 745, 1200
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 1339, 1371
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
Alw21I GWGCWC 3 cut(s) 694, 856, 904
Alw26I GTCTC 3 cut(s) 27, 1107, 1656
Alw44I GTGCAC 3 cut(s) 690, 852, 900
AlwNI CAGNNNCTG 1 cut(s) 319
Ama87I CYCGRG 4 cut(s) 137, 630, 2136, 2484
AoxI GGCC 7 cut(s) 129, 147, 431, 623, 662, 1378, 2133
ApaLI GTGCAC 3 cut(s) 690, 852, 900
ApeKI GCWGC 7 cut(s) 116, 1319, 1521, 2108, 2258, 2429, 2450
ApoI RAATTY 5 cut(s) 528, 1514, 1527, 2019, 2264
ArsI GACNNNNNNTTYG 4 cut(s) 1019, 1051, 2026, 2058
Asp700I GAANNNNTTC 4 cut(s) 473, 1514, 2194, 2521
AspLEI GCGC 3 cut(s) 7, 208, 1255
AspS9I GGNCC 3 cut(s) 623, 761, 2134
AsuC2I CCSGG 2 cut(s) 260, 2175
AsuHPI GGTGA 7 cut(s) 76, 824, 1552, 1741, 2189, 2190, 2517
AsuII TTCGAA 1 cut(s) 2045
AvaI CYCGRG 4 cut(s) 137, 630, 2136, 2484
AvaII GGWCC 1 cut(s) 761
AxyI CCTNAGG 1 cut(s) 1725
BaeGI GKGCMC 5 cut(s) 173, 694, 856, 904, 2032
BalI TGGCCA 1 cut(s) 131
BamHI GGATCC 4 cut(s) 1795, 1831, 2233, 2404
BanI GGYRCC 1 cut(s) 2027
BanII GRGCYC 1 cut(s) 1663
BbrPI CACGTG 1 cut(s) 2545
BbsI GAAGAC 3 cut(s) 400, 704, 1460
Bbv12I GWGCWC 3 cut(s) 694, 856, 904
BbvCI CCTCAGC 1 cut(s) 2382
BbvI GCAGC 7 cut(s) 103, 1331, 1508, 2095, 2245, 2416, 2437
BceAI ACGGC 5 cut(s) 52, 203, 253, 504, 606
BcgI CGANNNNNNTGC 6 cut(s) 1050, 1084, 1262, 1296, 2447, 2481
BciT130I CCWGG 4 cut(s) 109, 146, 747, 1202
BclI TGATCA 1 cut(s) 892
BcnI CCSGG 2 cut(s) 260, 2175
BcoDI GTCTC 3 cut(s) 27, 1107, 1656
BfaI CTAG 3 cut(s) 1287, 1427, 2310
BfmI CTRYAG 2 cut(s) 1165, 2213
BfrI CTTAAG 1 cut(s) 1187
BfuAI ACCTGC 6 cut(s) 95, 104, 1258, 2113, 2206, 2377
Bme1390I CCNGG 6 cut(s) 109, 146, 260, 747, 1202, 2175
Bme18I GGWCC 1 cut(s) 761
BmeT110I CYCGRG 4 cut(s) 137, 630, 2136, 2484
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 3 cut(s) 623, 761, 2134
BmiI GGNNCC 7 cut(s) 624, 763, 1797, 1833, 2029, 2235, 2406
BmrFI CCNGG 6 cut(s) 109, 146, 260, 747, 1202, 2175
BmsI GCATC 4 cut(s) 187, 1960, 2065, 2170
BpiI GAAGAC 3 cut(s) 400, 704, 1460
BpmI CTGGAG 4 cut(s) 143, 2145, 2319, 2490
Bpu10I CCTNAGC 4 cut(s) 1308, 2112, 2328, 2382
Bpu14I TTCGAA 1 cut(s) 2045
BpuMI CCSGG 2 cut(s) 260, 2175
BsaAI YACGTR 2 cut(s) 587, 2545
BsaBI GATNNNNATC 4 cut(s) 942, 1700, 1830, 2232
BsaHI GRCGYC 3 cut(s) 39, 72, 372
BsaI GGTCTC 2 cut(s) 1107, 1656
BsaJI CCNNGG 6 cut(s) 107, 144, 520, 1201, 1256, 2137
BsaWI WCCGGW 2 cut(s) 424, 968
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 1638, 1668
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 484
Bse118I RCCGGY 2 cut(s) 227, 1218
Bse1I ACTGG 5 cut(s) 160, 706, 1553, 2283, 2302
Bse21I CCTNAGG 1 cut(s) 1725
Bse3DI GCAATG 1 cut(s) 2505
Bse8I GATNNNNATC 4 cut(s) 942, 1700, 1830, 2232
BseBI CCWGG 4 cut(s) 109, 146, 747, 1202
BseDI CCNNGG 6 cut(s) 107, 144, 520, 1201, 1256, 2137
BseGI GGATG 5 cut(s) 385, 1398, 1831, 2296, 2509
BseJI GATNNNNATC 4 cut(s) 942, 1700, 1830, 2232
BseLI CCNNNNNNNGG 2 cut(s) 29, 484
BseMI GCAATG 1 cut(s) 2505
BseMII CTCAG 8 cut(s) 1078, 1299, 1739, 1784, 1794, 2126, 2342, 2396
BseNI ACTGG 5 cut(s) 160, 706, 1553, 2283, 2302
BseRI GAGGAG 7 cut(s) 319, 322, 359, 362, 1820, 2174, 2177
BseSI GKGCMC 5 cut(s) 173, 694, 856, 904, 2032
BseXI GCAGC 7 cut(s) 103, 1331, 1508, 2095, 2245, 2416, 2437
BseYI CCCAGC 3 cut(s) 819, 881, 1409
BsgI GTGCAG 3 cut(s) 163, 1533, 2525
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 7 cut(s) 131, 149, 433, 625, 664, 1380, 2135
BshNI GGYRCC 1 cut(s) 2027
BsiHKAI GWGCWC 3 cut(s) 694, 856, 904
BsiHKCI CYCGRG 4 cut(s) 137, 630, 2136, 2484
BsiSI CCGG 9 cut(s) 192, 203, 228, 259, 425, 665, 969, 1219, 2174
BsiWI CGTACG 1 cut(s) 587
BslFI GGGAC 4 cut(s) 127, 197, 1373, 1935
BslI CCNNNNNNNGG 2 cut(s) 29, 484
BsmAI GTCTC 3 cut(s) 27, 1107, 1656
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 4 cut(s) 127, 197, 1373, 1935
BsmI GAATGC 1 cut(s) 1430
BsnI GGCC 7 cut(s) 131, 149, 433, 625, 664, 1380, 2135
Bso31I GGTCTC 2 cut(s) 1107, 1656
BsoBI CYCGRG 4 cut(s) 137, 630, 2136, 2484
Bsp119I TTCGAA 1 cut(s) 2045
Bsp1286I GDGCHC 6 cut(s) 173, 694, 856, 904, 1663, 2032
Bsp1407I TGTACA 1 cut(s) 646
Bsp19I CCATGG 1 cut(s) 1256
BspACI CCGC 8 cut(s) 65, 150, 216, 240, 251, 327, 1090, 1381
BspANI GGCC 7 cut(s) 131, 149, 433, 625, 664, 1380, 2135
BspCNI CTCAG 8 cut(s) 1077, 1300, 1738, 1783, 1795, 2125, 2341, 2395
BspFNI CGCG 1 cut(s) 278
BspLI GGNNCC 7 cut(s) 624, 763, 1797, 1833, 2029, 2235, 2406
BspMAI CTGCAG 1 cut(s) 2217
BspMI ACCTGC 6 cut(s) 95, 104, 1258, 2113, 2206, 2377
BspT104I TTCGAA 1 cut(s) 2045
BspT107I GGYRCC 1 cut(s) 2027
BspTI CTTAAG 1 cut(s) 1187
BspTNI GGTCTC 2 cut(s) 1107, 1656
BsrBI CCGCTC 1 cut(s) 327
BsrDI GCAATG 1 cut(s) 2505
BsrFI RCCGGY 2 cut(s) 227, 1218
BsrGI TGTACA 1 cut(s) 646
BsrI ACTGG 5 cut(s) 160, 706, 1553, 2283, 2302
BssAI RCCGGY 2 cut(s) 227, 1218
BssECI CCNNGG 6 cut(s) 107, 144, 520, 1201, 1256, 2137
BssNI GRCGYC 3 cut(s) 39, 72, 372
BssT1I CCWWGG 1 cut(s) 1256
Bst2UI CCWGG 4 cut(s) 109, 146, 747, 1202
Bst4CI ACNGT 7 cut(s) 340, 906, 1020, 1336, 1492, 1650, 2373
Bst6I CTCTTC 3 cut(s) 571, 1779, 1815
BstACI GRCGYC 3 cut(s) 39, 72, 372
BstAFI CTTAAG 1 cut(s) 1187
BstAPI GCANNNNNTGC 3 cut(s) 51, 96, 1590
BstAUI TGTACA 1 cut(s) 646
BstBAI YACGTR 2 cut(s) 587, 2545
BstBI TTCGAA 1 cut(s) 2045
BstDSI CCRYGG 2 cut(s) 520, 1256
BstF5I GGATG 5 cut(s) 385, 1398, 1831, 2296, 2509
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 3 cut(s) 7, 208, 1255
BstMAI GTCTC 3 cut(s) 27, 1107, 1656
BstNI CCWGG 4 cut(s) 109, 146, 747, 1202
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 6 cut(s) 107, 144, 258, 745, 1200, 2173
BstSFI CTRYAG 2 cut(s) 1165, 2213
BstSLI GKGCMC 5 cut(s) 173, 694, 856, 904, 2032
BstSNI TACGTA 1 cut(s) 587
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 7 cut(s) 103, 1331, 1508, 2095, 2245, 2416, 2437
BstV2I GAAGAC 3 cut(s) 400, 704, 1460
BstX2I RGATCY 5 cut(s) 1795, 1831, 2053, 2233, 2404
BstXI CCANNNNNNTGG 2 cut(s) 872, 2039
BstYI RGATCY 5 cut(s) 1795, 1831, 2053, 2233, 2404
Bsu36I CCTNAGG 1 cut(s) 1725
BsuRI GGCC 7 cut(s) 131, 149, 433, 625, 664, 1380, 2135
BtgI CCRYGG 2 cut(s) 520, 1256
BtgZI GCGATG 1 cut(s) 210
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 5 cut(s) 385, 1398, 1831, 2296, 2509
BtsIMutI CAGTG 2 cut(s) 167, 1341
BveI ACCTGC 6 cut(s) 95, 104, 1258, 2113, 2206, 2377
CaiI CAGNNNCTG 1 cut(s) 319
CfoI GCGC 3 cut(s) 7, 208, 1255
Cfr10I RCCGGY 2 cut(s) 227, 1218
Cfr13I GGNCC 3 cut(s) 623, 761, 2134
CseI GACGC 4 cut(s) 28, 80, 284, 394
Csp6I GTAC 4 cut(s) 588, 647, 1493, 2295
CviQI GTAC 4 cut(s) 588, 647, 1493, 2295
DraIII CACNNNGTG 3 cut(s) 449, 1568, 2177
EaeI YGGCCR 3 cut(s) 129, 147, 1378
Eam1104I CTCTTC 3 cut(s) 571, 1779, 1815
EarI CTCTTC 3 cut(s) 571, 1779, 1815
EciI GGCGGA 1 cut(s) 54
Eco105I TACGTA 1 cut(s) 587
Eco130I CCWWGG 1 cut(s) 1256
Eco147I AGGCCT 1 cut(s) 433
Eco24I GRGCYC 1 cut(s) 1663
Eco31I GGTCTC 2 cut(s) 1107, 1656
Eco32I GATATC 1 cut(s) 1702
Eco47I GGWCC 1 cut(s) 761
Eco57I CTGAAG 5 cut(s) 488, 1334, 1461, 1736, 1982
Eco72I CACGTG 1 cut(s) 2545
Eco81I CCTNAGG 1 cut(s) 1725
Eco88I CYCGRG 4 cut(s) 137, 630, 2136, 2484
EcoO109I RGGNCCY 1 cut(s) 623
EcoRI GAATTC 1 cut(s) 1514
EcoRII CCWGG 4 cut(s) 107, 144, 745, 1200
EcoRV GATATC 1 cut(s) 1702
EcoT14I CCWWGG 1 cut(s) 1256
EcoT38I GRGCYC 1 cut(s) 1663
ErhI CCWWGG 1 cut(s) 1256
Esp3I CGTCTC 1 cut(s) 27
FalI AAGNNNNNCTT 6 cut(s) 816, 848, 1339, 1371, 1577, 1609
FaqI GGGAC 4 cut(s) 127, 197, 1373, 1935
FauI CCCGC 3 cut(s) 223, 320, 1083
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 1 cut(s) 892
FblI GTMKAC 1 cut(s) 841
FokI GGATG 5 cut(s) 392, 1405, 1838, 2303, 2516
FriOI GRGCYC 1 cut(s) 1663
FspBI CTAG 3 cut(s) 1287, 1427, 2310
GlaI GCGC 3 cut(s) 6, 207, 1254
GsaI CCCAGC 3 cut(s) 823, 885, 1413
GsuI CTGGAG 4 cut(s) 143, 2145, 2319, 2490
HaeIII GGCC 7 cut(s) 131, 149, 433, 625, 664, 1380, 2135
HapII CCGG 9 cut(s) 192, 203, 228, 259, 425, 665, 969, 1219, 2174
HgaI GACGC 4 cut(s) 28, 80, 284, 394
HhaI GCGC 3 cut(s) 7, 208, 1255
Hin1I GRCGYC 3 cut(s) 39, 72, 372
Hin6I GCGC 3 cut(s) 5, 206, 1253
HinP1I GCGC 3 cut(s) 5, 206, 1253
HpaII CCGG 9 cut(s) 192, 203, 228, 259, 425, 665, 969, 1219, 2174
HphI GGTGA 7 cut(s) 76, 824, 1552, 1741, 2189, 2190, 2517
Hpy166II GTNNAC 9 cut(s) 223, 235, 452, 678, 692, 842, 854, 902, 1646
Hpy8I GTNNAC 9 cut(s) 223, 235, 452, 678, 692, 842, 854, 902, 1646
Hpy99I CGWCG 5 cut(s) 32, 74, 278, 340, 374
HpyCH4III ACNGT 7 cut(s) 340, 906, 1020, 1336, 1492, 1650, 2373
HpyCH4IV ACGT 6 cut(s) 210, 372, 586, 688, 1957, 2544
HpySE526I ACGT 6 cut(s) 210, 372, 586, 688, 1957, 2544
Hsp92I GRCGYC 3 cut(s) 39, 72, 372
HspAI GCGC 3 cut(s) 5, 206, 1253
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 892
LmnI GCTCC 7 cut(s) 205, 289, 316, 2083, 2126, 2300, 2471
Lsp1109I GCAGC 7 cut(s) 103, 1331, 1508, 2095, 2245, 2416, 2437
LweI GCATC 4 cut(s) 187, 1960, 2065, 2170
MaeI CTAG 3 cut(s) 1287, 1427, 2310
MaeII ACGT 6 cut(s) 210, 372, 586, 688, 1957, 2544
MaeIII GTNAC 8 cut(s) 100, 420, 436, 548, 736, 1555, 1624, 1758
MbiI CCGCTC 1 cut(s) 327
MflI RGATCY 5 cut(s) 1795, 1831, 2053, 2233, 2404
MhlI GDGCHC 6 cut(s) 173, 694, 856, 904, 1663, 2032
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 7 cut(s) 457, 528, 1514, 1527, 1978, 2019, 2264
MluNI TGGCCA 1 cut(s) 131
MlyI GAGTC 9 cut(s) 55, 97, 149, 1056, 1206, 1766, 1817, 1957, 2018
MmeI TCCRAC 4 cut(s) 469, 1084, 1174, 1823
Mox20I TGGCCA 1 cut(s) 131
MreI CGCCGGCG 1 cut(s) 227
MroNI GCCGGC 1 cut(s) 227
MroXI GAANNNNTTC 4 cut(s) 473, 1514, 2194, 2521
MscI TGGCCA 1 cut(s) 131
MseI TTAA 2 cut(s) 1188, 1847
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 3 cut(s) 1383, 1521, 2258
MspCI CTTAAG 1 cut(s) 1187
MspI CCGG 9 cut(s) 192, 203, 228, 259, 425, 665, 969, 1219, 2174
MspR9I CCNGG 6 cut(s) 109, 146, 260, 747, 1202, 2175
Mva1269I GAATGC 1 cut(s) 1430
MvaI CCWGG 4 cut(s) 109, 146, 747, 1202
MvnI CGCG 1 cut(s) 278
NaeI GCCGGC 1 cut(s) 229
NciI CCSGG 2 cut(s) 260, 2175
NcoI CCATGG 1 cut(s) 1256
NdeI CATATG 1 cut(s) 1126
NgoMIV GCCGGC 1 cut(s) 227
NlaIV GGNNCC 7 cut(s) 624, 763, 1797, 1833, 2029, 2235, 2406
NmeAIII GCCGAG 1 cut(s) 100
NmuCI GTSAC 4 cut(s) 100, 736, 1624, 1758
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 2045
PaeI GCATGC 1 cut(s) 49
PaqCI CACCTGC 3 cut(s) 95, 2206, 2377
PceI AGGCCT 1 cut(s) 433
PcsI WCGNNNNNNNCGW 4 cut(s) 36, 216, 1654, 2550
PctI GAATGC 1 cut(s) 1430
PdiI GCCGGC 1 cut(s) 229
PdmI GAANNNNTTC 4 cut(s) 473, 1514, 2194, 2521
PfeI GAWTC 4 cut(s) 558, 742, 1720, 1859
Pfl23II CGTACG 1 cut(s) 587
PflFI GACNNNGTC 1 cut(s) 38
PfoI TCCNGGA 2 cut(s) 258, 745
PleI GAGTC 9 cut(s) 55, 97, 148, 1056, 1205, 1765, 1816, 1956, 2017
PmaCI CACGTG 1 cut(s) 2545
PmlI CACGTG 1 cut(s) 2545
PpsI GAGTC 9 cut(s) 55, 97, 148, 1056, 1205, 1765, 1816, 1956, 2017
Ppu21I YACGTR 2 cut(s) 587, 2545
Psp6I CCWGG 4 cut(s) 107, 144, 745, 1200
PspCI CACGTG 1 cut(s) 2545
PspFI CCCAGC 3 cut(s) 819, 881, 1409
PspGI CCWGG 4 cut(s) 107, 144, 745, 1200
PspLI CGTACG 1 cut(s) 587
PspN4I GGNNCC 7 cut(s) 624, 763, 1797, 1833, 2029, 2235, 2406
PspPI GGNCC 3 cut(s) 623, 761, 2134
PstI CTGCAG 1 cut(s) 2217
PstNI CAGNNNCTG 1 cut(s) 319
PsuI RGATCY 5 cut(s) 1795, 1831, 2053, 2233, 2404
PsyI GACNNNGTC 1 cut(s) 38
PvuII CAGCTG 2 cut(s) 1521, 2258
RsaI GTAC 4 cut(s) 589, 648, 1494, 2296
RsaNI GTAC 4 cut(s) 588, 647, 1493, 2295
SaqAI TTAA 2 cut(s) 1188, 1847
Sau96I GGNCC 3 cut(s) 623, 761, 2134
SchI GAGTC 9 cut(s) 55, 97, 149, 1056, 1206, 1766, 1817, 1957, 2018
ScrFI CCNGG 6 cut(s) 109, 146, 260, 747, 1202, 2175
SduI GDGCHC 6 cut(s) 173, 694, 856, 904, 1663, 2032
SfaNI GCATC 4 cut(s) 187, 1960, 2065, 2170
SfcI CTRYAG 2 cut(s) 1165, 2213
SfuI TTCGAA 1 cut(s) 2045
SgrAI CRCCGGYG 1 cut(s) 227
SinI GGWCC 1 cut(s) 761
SmlI CTYRAG 1 cut(s) 1187
SmoI CTYRAG 1 cut(s) 1187
SnaBI TACGTA 1 cut(s) 587
SphI GCATGC 1 cut(s) 49
Sse9I AATT 7 cut(s) 457, 528, 1514, 1527, 1978, 2019, 2264
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 8 cut(s) 65, 150, 216, 240, 251, 327, 1090, 1381
SspMI CTAG 3 cut(s) 1287, 1427, 2310
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 6 cut(s) 107, 144, 258, 745, 1200, 2173
StyI CCWWGG 1 cut(s) 1256
TaaI ACNGT 7 cut(s) 340, 906, 1020, 1336, 1492, 1650, 2373
TaiI ACGT 6 cut(s) 213, 375, 589, 691, 1960, 2547
TaqI TCGA 4 cut(s) 267, 1282, 1755, 2045
TasI AATT 7 cut(s) 457, 528, 1514, 1527, 1978, 2019, 2264
TatI WGTACW 1 cut(s) 646
TauI GCSGC 3 cut(s) 152, 254, 1383
TfiI GAWTC 4 cut(s) 558, 742, 1720, 1859
Tru1I TTAA 2 cut(s) 1188, 1847
Tru9I TTAA 2 cut(s) 1188, 1847
TscAI CASTG 2 cut(s) 167, 1341
TseFI GTSAC 4 cut(s) 100, 736, 1624, 1758
TseI GCWGC 7 cut(s) 116, 1319, 1521, 2108, 2258, 2429, 2450
Tsp45I GTSAC 4 cut(s) 100, 736, 1624, 1758
TspDTI ATGAA 9 cut(s) 1260, 1383, 1409, 1562, 1691, 1791, 1833, 1884, 2367
TspGWI ACGGA 2 cut(s) 45, 302
TspRI CASTG 2 cut(s) 167, 1341
Tth111I GACNNNGTC 1 cut(s) 38
Vha464I CTTAAG 1 cut(s) 1187
VneI GTGCAC 3 cut(s) 690, 852, 900
VpaK11BI GGWCC 1 cut(s) 761
XapI RAATTY 5 cut(s) 528, 1514, 1527, 2019, 2264
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 841
XmnI GAANNNNTTC 4 cut(s) 473, 1514, 2194, 2521
XspI CTAG 3 cut(s) 1287, 1427, 2310
ZraI GACGTC 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.