Rmu_sc0002393.1_g000020

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002393.1
OG0000644
Physical Location & Seq
Reverse (-)
90328 .. 93837
3510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002393.1_g000020.1.cds

Sequence Viewer

Length: 1506 bp
atgtctgacgtcatcaccggccttccctgcaactccgacggcctctgcatggtgtgcaaccaaaagccgtcgccggaggacctgttgtcttgcaccacttgcgcctcaccctggcacctgacttgcctctccgtccgtcccgaaacctcagccgacgctctggtgtggcagtgtcctgactgcacttctccgacggcttcggtaggtgccggaggagaaggaagctcctgcgatctggtggcggccatccgagctctggagaccgatgagtcgttgagtgaggctgagaaggcgaagaaacggcaggagctgatgtccaaaaacgttttagcaacgtccgatggcgacggcaacaaccgtgtgaagaggaccaacggcagtcgtaatgatgccctcgatattctcattaagagcttaacctgttcaatttgcttcgagcttccggagaggcctgttacgacaccttgcgggcacaacttctgcctcaaatgctttgagaaatgggtggcgcagggtaaacttacatgtgcaaattgccgccaatcgatcccctccactctgagaatcaactctaatcttgtgggaagcattcgtattgccaaggcatcacaacgcagcggctcaggggagtacctacctcagctggctcatcgcattagaaaccaggaccgtccagacaaagcatttacaaccgagcgagccaagaagcctgggaaagctaatgcctcaagtggaaggatctttgtgacatcagctccggatcattttgggcccataaccgccgaatatgatcctgagaagaataagggtgtggcggtgggggagacttgggggcataggattgaatgtagacaatggggtattcatcgccctatggtttccgggatttctgggcagtgcaagtatggcgcccaatcagtagttctctcaggagggtatatagacgatgaagatcacggtgaatggttcatttacaccgggagtggtggcaaggatttgagtggaaacaagcgtaccaacaagcaacacagtaaagatcagacgttcacaaagcttaatcagtcactcaggctaagttgcctaaatggttatcctgttcgagtaactcgagttgagcccaagcccaaaggccaaagcccaaacccaagctatagaaccagaagcccaactcccttgcctagccatcgccaccagcgtcgtcggcagctctggtggtcgacccttccaccatgccatcaattgtcaatcagcgaaccacccagacaagtacttctccggcgttcaaccggacaactgatccaggctaatccagcctcccaccgctacctggaccccaacccgagttcccggtccttgttacctgagcaatcgcccgctgcgactcaaccaccaccaccttcaataaaacgcagaaccctctggcaacctcgacagagctatgaagccgccgtcgatcaccaagaacaagacttggcacctagacgacccagaactcctccaccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

501

Amino Acids

55.21

Weight (kDa)

9.24

Isoelectric Point (pI)

61.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 268
AatII GACGTC 1 cut(s) 12
AccB1I GGYRCC 4 cut(s) 114, 206, 908, 1474
AccI GTMKAC 2 cut(s) 850, 1217
AccIII TCCGGA 2 cut(s) 442, 757
AciI CCGC 9 cut(s) 242, 468, 538, 618, 780, 815, 1321, 1374, 1446
AclI AACGTT 1 cut(s) 324
AclWI GGATC 5 cut(s) 541, 746, 768, 785, 1291
AcoI YGGCCR 1 cut(s) 243
AcyI GRCGYC 2 cut(s) 9, 909
AfaI GTAC 3 cut(s) 632, 1015, 1269
AfiI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 1327
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 4 cut(s) 426, 845, 1284, 1401
AhdI GACNNNNNGTC 1 cut(s) 85
AjnI CCWGG 5 cut(s) 110, 663, 709, 1299, 1326
AjuI GAANNNNNNNTTGG 2 cut(s) 906, 938
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 2 cut(s) 254, 818
AlwI GGATC 5 cut(s) 541, 746, 768, 785, 1291
AlwNI CAGNNNCTG 1 cut(s) 310
Ama87I CYCGRG 2 cut(s) 1107, 1339
Aor13HI TCCGGA 2 cut(s) 442, 757
AoxI GGCC 6 cut(s) 19, 40, 243, 449, 770, 1129
ApaI GGGCCC 1 cut(s) 774
ApeKI GCWGC 3 cut(s) 615, 1204, 1376
AspLEI GCGC 3 cut(s) 104, 511, 911
AspS9I GGNCC 7 cut(s) 79, 369, 667, 770, 771, 1330, 1350
AsuC2I CCSGG 3 cut(s) 883, 979, 1348
AsuHPI GGTGA 4 cut(s) 7, 99, 971, 1448
AvaI CYCGRG 2 cut(s) 1107, 1339
AvaII GGWCC 5 cut(s) 79, 369, 667, 1330, 1350
BaeGI GKGCMC 2 cut(s) 474, 774
BaeI ACNNNNGTAYC 2 cut(s) 997, 1030
BanI GGYRCC 4 cut(s) 114, 206, 908, 1474
BanII GRGCYC 3 cut(s) 256, 774, 1119
Bbv12I GWGCWC 1 cut(s) 256
BbvCI CCTCAGC 2 cut(s) 148, 639
BbvI GCAGC 3 cut(s) 627, 1216, 1363
BccI CCATC 4 cut(s) 254, 335, 1191, 1242
BceAI ACGGC 7 cut(s) 52, 55, 210, 317, 364, 391, 1433
BciT130I CCWGG 5 cut(s) 112, 665, 711, 1301, 1328
BcnI CCSGG 3 cut(s) 883, 979, 1348
BcoDI GTCTC 2 cut(s) 254, 818
BfaI CTAG 2 cut(s) 1179, 1479
BfmI CTRYAG 1 cut(s) 1150
BfoI RGCGCY 1 cut(s) 912
BisI GCNGC 7 cut(s) 243, 538, 616, 619, 1205, 1377, 1446
BlsI GCNGC 7 cut(s) 244, 539, 617, 620, 1206, 1378, 1447
BmcAI AGTACT 1 cut(s) 1269
Bme1390I CCNGG 8 cut(s) 112, 665, 711, 883, 979, 1301, 1328, 1348
Bme18I GGWCC 5 cut(s) 79, 369, 667, 1330, 1350
BmeRI GACNNNNNGTC 1 cut(s) 85
BmeT110I CYCGRG 2 cut(s) 1107, 1339
BmgT120I GGNCC 7 cut(s) 79, 369, 667, 770, 771, 1330, 1350
BmiI GGNNCC 6 cut(s) 116, 208, 772, 910, 1332, 1476
BmrFI CCNGG 8 cut(s) 112, 665, 711, 883, 979, 1301, 1328, 1348
BmsI GCATC 2 cut(s) 379, 614
BpmI CTGGAG 1 cut(s) 278
Bpu10I CCTNAGC 4 cut(s) 148, 622, 639, 1362
BpuEI CTTGAG 1 cut(s) 712
BpuMI CCSGG 3 cut(s) 883, 979, 1348
Bsa29I ATCGAT 1 cut(s) 545
BsaBI GATNNNNATC 1 cut(s) 951
BsaHI GRCGYC 2 cut(s) 9, 909
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 3 cut(s) 110, 600, 710
BsaWI WCCGGW 3 cut(s) 442, 757, 1286
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 299, 329, 739, 769
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 111, 256, 1327
Bse118I RCCGGY 1 cut(s) 17
Bse8I GATNNNNATC 1 cut(s) 951
BseAI TCCGGA 2 cut(s) 442, 757
BseBI CCWGG 5 cut(s) 112, 665, 711, 1301, 1328
BseCI ATCGAT 1 cut(s) 545
BseDI CCNNGG 3 cut(s) 110, 600, 710
BseGI GGATG 1 cut(s) 246
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 1327
BseMII CTCAG 9 cut(s) 162, 276, 551, 636, 653, 786, 942, 1081, 1353
BseRI GAGGAG 2 cut(s) 228, 1485
BseSI GKGCMC 2 cut(s) 474, 774
BseXI GCAGC 3 cut(s) 627, 1216, 1363
BsgI GTGCAG 1 cut(s) 166
BshFI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1131
BshNI GGYRCC 4 cut(s) 114, 206, 908, 1474
BshVI ATCGAT 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 256
BsiHKCI CYCGRG 2 cut(s) 1107, 1339
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 1327
BsmAI GTCTC 2 cut(s) 254, 818
BsmFI GGGAC 1 cut(s) 123
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1131
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 2 cut(s) 1107, 1339
Bsp120I GGGCCC 1 cut(s) 770
Bsp1286I GDGCHC 4 cut(s) 256, 474, 774, 1119
Bsp13I TCCGGA 2 cut(s) 442, 757
Bsp143I GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1296, 1453
BspACI CCGC 9 cut(s) 242, 468, 538, 618, 780, 815, 1321, 1374, 1446
BspANI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1131
BspCNI CTCAG 9 cut(s) 161, 277, 552, 635, 652, 787, 941, 1080, 1354
BspDI ATCGAT 1 cut(s) 545
BspEI TCCGGA 2 cut(s) 442, 757
BspLI GGNNCC 6 cut(s) 116, 208, 772, 910, 1332, 1476
BspPI GGATC 5 cut(s) 541, 746, 768, 785, 1291
BspT107I GGYRCC 4 cut(s) 114, 206, 908, 1474
BspTNI GGTCTC 1 cut(s) 254
BsrFI RCCGGY 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 3 cut(s) 110, 600, 710
BssMI GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1296, 1453
BssNI GRCGYC 2 cut(s) 9, 909
BssT1I CCWWGG 1 cut(s) 600
Bst2UI CCWGG 5 cut(s) 112, 665, 711, 1301, 1328
Bst4CI ACNGT 5 cut(s) 359, 671, 959, 1031, 1503
Bst6I CTCTTC 1 cut(s) 359
BstACI GRCGYC 2 cut(s) 9, 909
BstAPI GCANNNNNTGC 2 cut(s) 54, 99
BstC8I GCNNGC 4 cut(s) 470, 645, 699, 1374
BstF5I GGATG 1 cut(s) 246
BstH2I RGCGCY 1 cut(s) 912
BstHHI GCGC 3 cut(s) 104, 511, 911
BstKTI GATC 9 cut(s) 235, 549, 741, 763, 793, 955, 1039, 1299, 1456
BstMAI GTCTC 2 cut(s) 254, 818
BstMBI GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1296, 1453
BstMWI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 251, 290, 489, 906, 1201, 1310
BstNI CCWGG 5 cut(s) 112, 665, 711, 1301, 1328
BstNSI RCATGY 1 cut(s) 528
BstSCI CCNGG 8 cut(s) 110, 663, 709, 881, 977, 1299, 1326, 1346
BstSFI CTRYAG 1 cut(s) 1150
BstSLI GKGCMC 2 cut(s) 474, 774
BstV1I GCAGC 3 cut(s) 627, 1216, 1363
BstX2I RGATCY 1 cut(s) 738
BstYI RGATCY 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 545
BsuRI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1131
BsuTUI ATCGAT 1 cut(s) 545
BtgZI GCGATG 3 cut(s) 635, 851, 1169
BtsCI GGATG 1 cut(s) 246
BtsI GCAGTG 2 cut(s) 176, 902
BtsIMutI CAGTG 2 cut(s) 176, 902
Cac8I GCNNGC 4 cut(s) 470, 645, 699, 1374
CaiI CAGNNNCTG 1 cut(s) 310
CfoI GCGC 3 cut(s) 104, 511, 911
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 7 cut(s) 79, 369, 667, 770, 771, 1330, 1350
ClaI ATCGAT 1 cut(s) 545
CseI GACGC 2 cut(s) 164, 1184
Csp6I GTAC 3 cut(s) 631, 1014, 1268
CviAII CATG 3 cut(s) 49, 525, 1230
CviQI GTAC 3 cut(s) 631, 1014, 1268
DinI GGCGCC 1 cut(s) 910
DpnI GATC 9 cut(s) 234, 548, 740, 762, 792, 954, 1038, 1298, 1455
DpnII GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1296, 1453
DrdI GACNNNNNNGTC 1 cut(s) 268
DriI GACNNNNNGTC 1 cut(s) 85
DseDI GACNNNNNNGTC 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 359
Eam1105I GACNNNNNGTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 359
Ecl136II GAGCTC 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 600
Eco147I AGGCCT 1 cut(s) 451
Eco24I GRGCYC 3 cut(s) 256, 774, 1119
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 5 cut(s) 79, 369, 667, 1330, 1350
Eco53kI GAGCTC 1 cut(s) 254
Eco88I CYCGRG 2 cut(s) 1107, 1339
EcoICRI GAGCTC 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 5 cut(s) 110, 663, 709, 1299, 1326
EcoT14I CCWWGG 1 cut(s) 600
EcoT38I GRGCYC 3 cut(s) 256, 774, 1119
EgeI GGCGCC 1 cut(s) 910
EheI GGCGCC 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 3 cut(s) 52, 528, 1233
FaqI GGGAC 1 cut(s) 123
FatI CATG 3 cut(s) 48, 524, 1229
FauI CCCGC 2 cut(s) 461, 1381
FblI GTMKAC 2 cut(s) 850, 1217
Fnu4HI GCNGC 7 cut(s) 243, 538, 616, 619, 1205, 1377, 1446
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 3 cut(s) 256, 774, 1119
Fsp4HI GCNGC 7 cut(s) 243, 538, 616, 619, 1205, 1377, 1446
FspBI CTAG 2 cut(s) 1179, 1479
GlaI GCGC 3 cut(s) 103, 510, 910
GluI GCNGC 7 cut(s) 243, 538, 616, 619, 1205, 1377, 1446
GsuI CTGGAG 1 cut(s) 278
HaeII RGCGCY 1 cut(s) 912
HaeIII GGCC 6 cut(s) 21, 42, 245, 451, 772, 1131
HgaI GACGC 2 cut(s) 164, 1184
HhaI GCGC 3 cut(s) 104, 511, 911
Hin1I GRCGYC 2 cut(s) 9, 909
Hin1II CATG 3 cut(s) 52, 528, 1233
Hin6I GCGC 3 cut(s) 102, 509, 909
HinP1I GCGC 3 cut(s) 102, 509, 909
HincII GTYRAC 1 cut(s) 1218
HindII GTYRAC 1 cut(s) 1218
HindIII AAGCTT 1 cut(s) 1052
HinfI GANTC 3 cut(s) 269, 564, 1381
HphI GGTGA 4 cut(s) 7, 99, 971, 1448
Hpy166II GTNNAC 4 cut(s) 518, 851, 1047, 1218
Hpy188I TCNGA 7 cut(s) 7, 37, 192, 251, 340, 561, 1041
Hpy188III TCNNGA 8 cut(s) 140, 176, 257, 443, 674, 758, 794, 930
Hpy8I GTNNAC 4 cut(s) 518, 851, 1047, 1218
Hpy99I CGWCG 8 cut(s) 41, 73, 158, 196, 350, 1200, 1203, 1454
HpyAV CCTTC 6 cut(s) 32, 212, 283, 729, 1232, 1407
HpyCH4III ACNGT 5 cut(s) 359, 671, 959, 1031, 1503
HpyCH4IV ACGT 4 cut(s) 9, 324, 335, 1043
HpyCH4V TGCA 7 cut(s) 30, 48, 57, 93, 183, 530, 900
HpyF10VI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 251, 290, 489, 906, 1201, 1310
HpySE526I ACGT 4 cut(s) 9, 324, 335, 1043
Hsp92I GRCGYC 2 cut(s) 9, 909
Hsp92II CATG 3 cut(s) 52, 528, 1233
HspAI GCGC 3 cut(s) 102, 509, 909
KasI GGCGCC 1 cut(s) 908
Kpn2I TCCGGA 2 cut(s) 442, 757
Kzo9I GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1296, 1453
LmnI GCTCC 3 cut(s) 230, 307, 760
Lsp1109I GCAGC 3 cut(s) 627, 1216, 1363
LweI GCATC 2 cut(s) 379, 614
MaeI CTAG 2 cut(s) 1179, 1479
MaeII ACGT 4 cut(s) 9, 324, 335, 1043
MaeIII GTNAC 5 cut(s) 454, 745, 1062, 1102, 1356
MalI GATC 9 cut(s) 234, 548, 740, 762, 792, 954, 1038, 1298, 1455
MboI GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1296, 1453
MboII GAAGA 4 cut(s) 307, 376, 811, 962
MfeI CAATTG 1 cut(s) 1238
MflI RGATCY 1 cut(s) 738
MhlI GDGCHC 4 cut(s) 256, 474, 774, 1119
MluCI AATT 3 cut(s) 426, 532, 1238
Mly113I GGCGCC 1 cut(s) 909
MlyI GAGTC 2 cut(s) 278, 1375
MmeI TCCRAC 2 cut(s) 60, 215
MroI TCCGGA 2 cut(s) 442, 757
MseI TTAA 3 cut(s) 408, 416, 1056
MspA1I CMGCKG 3 cut(s) 618, 643, 1376
MspR9I CCNGG 8 cut(s) 112, 665, 711, 883, 979, 1301, 1328, 1348
MunI CAATTG 1 cut(s) 1238
Mva1269I GAATGC 1 cut(s) 588
MvaI CCWGG 5 cut(s) 112, 665, 711, 1301, 1328
MwoI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 251, 290, 489, 906, 1201, 1310
NarI GGCGCC 1 cut(s) 909
NciI CCSGG 3 cut(s) 883, 979, 1348
NdeII GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1296, 1453
NlaIII CATG 3 cut(s) 52, 528, 1233
NlaIV GGNNCC 6 cut(s) 116, 208, 772, 910, 1332, 1476
NmuCI GTSAC 2 cut(s) 745, 1062
NspI RCATGY 1 cut(s) 528
PaeR7I CTCGAG 1 cut(s) 1107
PceI AGGCCT 1 cut(s) 451
PciI ACATGT 1 cut(s) 524
PcsI WCGNNNNNNNCGW 3 cut(s) 1105, 1192, 1376
PctI GAATGC 1 cut(s) 588
PfeI GAWTC 1 cut(s) 564
PfoI TCCNGGA 1 cut(s) 881
PkrI GCNGC 7 cut(s) 244, 539, 617, 620, 1206, 1378, 1447
PleI GAGTC 2 cut(s) 277, 1375
PluTI GGCGCC 1 cut(s) 912
PpsI GAGTC 2 cut(s) 277, 1375
PpuMI RGGWCCY 1 cut(s) 79
PscI ACATGT 1 cut(s) 524
Psp124BI GAGCTC 1 cut(s) 256
Psp1406I AACGTT 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 5 cut(s) 110, 663, 709, 1299, 1326
PspGI CCWGG 5 cut(s) 110, 663, 709, 1299, 1326
PspN4I GGNNCC 6 cut(s) 116, 208, 772, 910, 1332, 1476
PspOMI GGGCCC 1 cut(s) 770
PspPI GGNCC 7 cut(s) 79, 369, 667, 770, 771, 1330, 1350
PspPPI RGGWCCY 1 cut(s) 79
PspXI VCTCGAGB 1 cut(s) 1107
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 738
PvuII CAGCTG 1 cut(s) 643
RsaI GTAC 3 cut(s) 632, 1015, 1269
RsaNI GTAC 3 cut(s) 631, 1014, 1268
SacI GAGCTC 1 cut(s) 256
SalI GTCGAC 1 cut(s) 1216
SaqAI TTAA 3 cut(s) 408, 416, 1056
SatI GCNGC 7 cut(s) 243, 538, 616, 619, 1205, 1377, 1446
Sau3AI GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1296, 1453
Sau96I GGNCC 7 cut(s) 79, 369, 667, 770, 771, 1330, 1350
ScaI AGTACT 1 cut(s) 1269
SchI GAGTC 2 cut(s) 278, 1375
ScrFI CCNGG 8 cut(s) 112, 665, 711, 883, 979, 1301, 1328, 1348
SduI GDGCHC 4 cut(s) 256, 474, 774, 1119
SfaNI GCATC 2 cut(s) 379, 614
SfcI CTRYAG 1 cut(s) 1150
SfoI GGCGCC 1 cut(s) 910
Sfr274I CTCGAG 1 cut(s) 1107
SinI GGWCC 5 cut(s) 79, 369, 667, 1330, 1350
SlaI CTCGAG 1 cut(s) 1107
SmlI CTYRAG 2 cut(s) 727, 1107
SmoI CTYRAG 2 cut(s) 727, 1107
Sse9I AATT 3 cut(s) 426, 532, 1238
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 9 cut(s) 242, 468, 538, 618, 780, 815, 1321, 1374, 1446
SspDI GGCGCC 1 cut(s) 908
SspMI CTAG 2 cut(s) 1179, 1479
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 8 cut(s) 110, 663, 709, 881, 977, 1299, 1326, 1346
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 5 cut(s) 359, 671, 959, 1031, 1503
TaiI ACGT 4 cut(s) 12, 327, 338, 1046
TaqI TCGA 8 cut(s) 396, 435, 545, 1099, 1108, 1217, 1429, 1452
TaqII GACCGA 1 cut(s) 278
TasI AATT 3 cut(s) 426, 532, 1238
TatI WGTACW 1 cut(s) 1267
TauI GCSGC 4 cut(s) 245, 540, 621, 1448
TfiI GAWTC 1 cut(s) 564
Tru1I TTAA 3 cut(s) 408, 416, 1056
Tru9I TTAA 3 cut(s) 408, 416, 1056
TscAI CASTG 2 cut(s) 176, 902
TseFI GTSAC 2 cut(s) 745, 1062
TseI GCWGC 3 cut(s) 615, 1204, 1376
Tsp45I GTSAC 2 cut(s) 745, 1062
TspDTI ATGAA 4 cut(s) 854, 958, 963, 1455
TspGWI ACGGA 2 cut(s) 121, 125
TspRI CASTG 2 cut(s) 176, 902
VpaK11BI GGWCC 5 cut(s) 79, 369, 667, 1330, 1350
XceI RCATGY 1 cut(s) 528
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XhoI CTCGAG 1 cut(s) 1107
XmiI GTMKAC 2 cut(s) 850, 1217
XspI CTAG 2 cut(s) 1179, 1479
ZraI GACGTC 1 cut(s) 10
ZrmI AGTACT 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.