Rroxscaffold_3G00266210

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000644
Physical Location & Seq
Reverse (-)
59566285 .. 59571670
5386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266210.1

Sequence Viewer

Length: 2151 bp
ATGGCGCACGTTAGTCAGCTGCCGTGCGACGGAGACGGCGTCTGCATGCTCTGCAAAACGACGCCGTCGCACGAGGACCAGCTCACCTGCAGGACCTGCGTCACGCCGTGGCACGTCAGCTGCCTCTCGTCCCGTCCCGAATCGCTGGCTTCCACTCTCCAGTGGGAGTGCCCGGACTGTTCCTCGCTCTCCGGGAACACGCCGGCCGTGAAATCCGCCGTCTCCGGCGAGCTGATTGCCGCGATCCGGGCGATCGAGGCCGATGCGGCGCTGACGGAGCAGCAGAAGGCGAGTAAGCGACAGGAGCTGATGAGCGGGAAGGCTCTGCCGGAGGAAGAAGAAGGCGCCGGCAGGAAGGGAGTAAACGACGGCGTTTTGGATGGGATTTTCAATTGCTCGTTCTGTATGCAACTACCGGAGAGACCTGTTACGACACCATGTGGCCACAATTTCTGCTTGAAGTGCTTCCAGAAATGGGTTGGACAAGGGAAGCGTACTTGTGCAAAGTGCCGCCATCCCATTCCTTCCAAAATGGCAAGTCAACCCCGCATCAATTCTTCACTTGTAGTTGCCATACGTATGGCTAAGATGTCAAAATCATATGTTACTGAGGGGCCTGCTAAGGTATACCACTTTGTCCGAAACCAAGACAGACCTGACAAAGCTTTCACTACCGAGCGGGCACAGAAAACTGGGAAATCTAATGCTGCAAGTGGCAAGATATTTGTCACAATTCCAGCTGATCATTTTGGACCTATACCTGCTGAATATGATCCTGAGAGAAACCAAGGTGTGCTGGTTGGGGAATGCTGGGAGGATAGGCTTGAATGTCGACAGTGGGGTGCACACTTTCCCCATGTTGCTGGTATAGCTGGACAGTCTAACCATGGTGCACAGTCAGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATACACAGGAAGTGGTGGAAGGGATTTGAGTGGGAATAAGCGAACAAACAAGGATCAGTCATTTGACCAGACATTTGAGAAATATAACAAGGCTCTCCAAGTTAGTTGCCTGAAAGGGTATCCTGTACGGGTGGTAAGATCACACAAGGAGAAGCGCTCCTCATATGCCCCTGAGAAAGGGTTGCGGTACGATGGAGTCTATAGAATTGAAAAATGTTGGACAAAGGTGGGAATCCAGGGGTTTAAGGTCTGCCGGTATCTTTTTGTTAGATGTGACAATGAACCTGCACCATGGACGAGTGATGAACATGGGGATCGGCCTAGACCTTTACCTCCAATACCTGAGCTGAAGAAAGCTACAAGTATAATAGAGAGGAAGTCAAGTCCATCATGGGACTTTGAGGAGAAAGATGGCTGCTGGAAGTGGAAAAAGTCACCACCTATCAGTAAAAAAACTGTTCAGACATTAGATCCTGCTGATCAAAAGAGCTGCCTTGTTTGTGGGGAGGTCATGACATTACCAATGACCACACCTTGTGCTCATAACTTTTGCAAGTCCTGTTTGGAAGGCGCTTTTGCGGGAAAGAGCTTGGTTTGTAACTTGTGCATTTTGCCTTCATTTCATTCAGAAGATAATGTTACTGTTCCTCCATTTTATCTGATACGAATGGAGATATGTAATCTAGCCTTGGTGAACAGAGAGTTAATGGATGCGATTGAGTCACTTAAGTCACAGAATGAAGAGGAAGAGAATGAGGATTCAGCTGAAGAAATGAGCGAAGATATGGATAATTCAGACAATGAAATTAGCACTGAAATTGTTGAAGAAGCAGGTGTTAAAGAATTTGCAGAGAATTCCCCAACACAGTGCAATCCTGAAAGGAGTCCCAAACGACAGAAAGTTGATGTTGGTAAAGGCTCACTGAAAATCAATGATGAGGAGGTAAATGTAATTCCTGTCTTCCAGACTAATGGCAAGGGCTCACCAAAAACCGATGCTGAGGAGGTAAATGAAAATCCTGTTGCCCAGACTAATGGCAAAGGGTCAGCGGCCTCAGTTGCAAAATGCGGCGAGCACGATGCTGGTCCGGGTGGCGTTGCAGCAAATACTAGAGGGAGGACGAAGGCTGAAGAAGTTGTGGGTAAGGATAATGGTTCCCCATCAAGTCCTCTGCTTATGCAATCAGATTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

716

Amino Acids

78.67

Weight (kDa)

5.86

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 131 - 171 1.5e-06 Ring finger domain
zf-C3HC4_3 PF13920 131 - 175 3.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 132 - 170 2.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 132 - 170 2.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 132 - 170 5e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 262 - 411 1.4e-49 SAD/SRA domain
zf-RING_UBOX PF13445 482 - 515 2.8e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 95, 1775
Acc36I ACCTGC 5 cut(s) 95, 104, 769, 1246, 1775
Acc65I GGTACC 1 cut(s) 918
AccB1I GGYRCC 2 cut(s) 344, 918
AccBSI CCGCTC 2 cut(s) 315, 679
AccI GTMKAC 2 cut(s) 627, 832
AccII CGCG 1 cut(s) 242
AclWI GGATC 6 cut(s) 238, 767, 942, 1014, 1275, 1418
AcoI YGGCCR 2 cut(s) 204, 442
AcsI RAATTY 2 cut(s) 1796, 1807
AcuI CTGAAG 3 cut(s) 1322, 1740, 2103
AcyI GRCGYC 3 cut(s) 39, 62, 345
AdeI CACNNNGTG 3 cut(s) 108, 440, 1490
AfaI GTAC 4 cut(s) 496, 920, 1080, 1142
AfeI AGCGCT 1 cut(s) 1109
AfiI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
AflII CTTAAG 1 cut(s) 1679
AgsI TTSAA 6 cut(s) 391, 460, 827, 1163, 1778, 2147
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 1 cut(s) 1188
Alw21I GWGCWC 4 cut(s) 847, 895, 1495, 2031
Alw26I GTCTC 3 cut(s) 27, 226, 415
Alw44I GTGCAC 2 cut(s) 843, 891
AlwI GGATC 6 cut(s) 238, 767, 942, 1014, 1275, 1418
AlwNI CAGNNNCTG 2 cut(s) 96, 307
Aor51HI AGCGCT 1 cut(s) 1109
AoxI GGCC 6 cut(s) 204, 258, 442, 614, 1271, 2004
ApaLI GTGCAC 2 cut(s) 843, 891
ApeKI GCWGC 7 cut(s) 19, 120, 280, 707, 1368, 1443, 2054
ApoI RAATTY 2 cut(s) 1796, 1807
ArsI GACNNNNNNTTYG 2 cut(s) 1010, 1042
Asp700I GAANNNNTTC 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 918
AspLEI GCGC 5 cut(s) 7, 271, 347, 1110, 1526
AspS9I GGNCC 5 cut(s) 76, 93, 614, 752, 2039
AsuC2I CCSGG 4 cut(s) 173, 193, 248, 2043
AsuHPI GGTGA 4 cut(s) 76, 1380, 1657, 1929
AvaII GGWCC 4 cut(s) 76, 93, 752, 2039
BaeGI GKGCMC 4 cut(s) 173, 685, 847, 895
BalI TGGCCA 1 cut(s) 444
BanI GGYRCC 2 cut(s) 344, 918
BanII GRGCYC 1 cut(s) 1937
BauI CACGAG 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 1906
Bbv12I GWGCWC 4 cut(s) 847, 895, 1495, 2031
BbvCI CCTCAGC 1 cut(s) 1953
BbvI GCAGC 7 cut(s) 6, 107, 292, 694, 1355, 1430, 2066
BccI CCATC 6 cut(s) 374, 522, 1139, 1348, 1358, 2122
BceAI ACGGC 7 cut(s) 7, 49, 52, 91, 191, 203, 385
BcgI CGANNNNNNTGC 6 cut(s) 218, 245, 252, 279, 2015, 2049
BciT130I CCWGG 1 cut(s) 1190
BciVI GTATCC 1 cut(s) 1083
BclI TGATCA 2 cut(s) 742, 1432
BcnI CCSGG 4 cut(s) 173, 193, 248, 2043
BcoDI GTCTC 3 cut(s) 27, 226, 415
BfaI CTAG 3 cut(s) 1275, 1637, 2064
BfmI CTRYAG 2 cut(s) 88, 1153
BfoI RGCGCY 4 cut(s) 272, 348, 1111, 1527
BfrI CTTAAG 1 cut(s) 1679
BfuAI ACCTGC 5 cut(s) 95, 104, 769, 1246, 1775
BfuI GTATCC 1 cut(s) 1083
BglI GCCNNNNNGGC 1 cut(s) 266
Bme1390I CCNGG 5 cut(s) 173, 193, 248, 1190, 2043
Bme18I GGWCC 4 cut(s) 76, 93, 752, 2039
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 5 cut(s) 76, 93, 614, 752, 2039
BmiI GGNNCC 4 cut(s) 346, 615, 920, 2110
BmrFI CCNGG 5 cut(s) 173, 193, 248, 1190, 2043
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 5 cut(s) 253, 558, 1654, 1939, 2023
BmuI ACTGGG 1 cut(s) 702
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 1906
BplI GAGNNNNNCTC 2 cut(s) 1094, 1126
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 3 cut(s) 621, 1296, 1953
BpuMI CCSGG 4 cut(s) 173, 193, 248, 2043
BsaAI YACGTR 1 cut(s) 578
BsaBI GATNNNNATC 1 cut(s) 933
BsaHI GRCGYC 3 cut(s) 39, 62, 345
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 6 cut(s) 107, 787, 886, 1189, 1244, 1641
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 1585, 1615
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
Bse118I RCCGGY 3 cut(s) 202, 347, 1206
Bse1I ACTGG 2 cut(s) 160, 697
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 1 cut(s) 1190
BseDI CCNNGG 6 cut(s) 107, 787, 886, 1189, 1244, 1641
BseGI GGATG 3 cut(s) 385, 514, 1669
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
BseMII CTCAG 6 cut(s) 600, 768, 1116, 1287, 1944, 2022
BseNI ACTGG 2 cut(s) 160, 697
BseRI GAGGAG 4 cut(s) 1102, 1370, 1907, 1970
BseSI GKGCMC 4 cut(s) 173, 685, 847, 895
BseX3I CGGCCG 1 cut(s) 204
BseXI GCAGC 7 cut(s) 6, 107, 292, 694, 1355, 1430, 2066
BseYI CCCAGC 1 cut(s) 810
BsgI GTGCAG 1 cut(s) 1224
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 2 cut(s) 207, 255
BshFI GGCC 6 cut(s) 206, 260, 444, 616, 1273, 2006
BshNI GGYRCC 2 cut(s) 344, 918
BsiEI CGRYCG 2 cut(s) 207, 255
BsiHKAI GWGCWC 4 cut(s) 847, 895, 1495, 2031
BslFI GGGAC 4 cut(s) 115, 120, 1361, 1824
BslI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
BsmAI GTCTC 3 cut(s) 27, 226, 415
BsmBI CGTCTC 2 cut(s) 27, 226
BsmFI GGGAC 4 cut(s) 115, 120, 1361, 1824
BsmI GAATGC 1 cut(s) 812
BsnI GGCC 6 cut(s) 206, 260, 444, 616, 1273, 2006
Bso31I GGTCTC 1 cut(s) 415
Bsp1286I GDGCHC 7 cut(s) 173, 685, 847, 895, 1495, 1937, 2031
Bsp19I CCATGG 2 cut(s) 886, 1244
BspANI GGCC 6 cut(s) 206, 260, 444, 616, 1273, 2006
BspCNI CTCAG 6 cut(s) 601, 769, 1117, 1288, 1945, 2021
BspFNI CGCG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 1464
BspLI GGNNCC 4 cut(s) 346, 615, 920, 2110
BspMAI CTGCAG 1 cut(s) 92
BspMI ACCTGC 5 cut(s) 95, 104, 769, 1246, 1775
BspPI GGATC 6 cut(s) 238, 767, 942, 1014, 1275, 1418
BspT107I GGYRCC 2 cut(s) 344, 918
BspTI CTTAAG 1 cut(s) 1679
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 2 cut(s) 315, 679
BsrFI RCCGGY 3 cut(s) 202, 347, 1206
BsrI ACTGG 2 cut(s) 160, 697
BssAI RCCGGY 3 cut(s) 202, 347, 1206
BssECI CCNNGG 6 cut(s) 107, 787, 886, 1189, 1244, 1641
BssNAI GTATAC 1 cut(s) 628
BssNI GRCGYC 3 cut(s) 39, 62, 345
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 4 cut(s) 787, 886, 1244, 1641
Bst1107I GTATAC 1 cut(s) 628
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 1190
Bst4CI ACNGT 7 cut(s) 179, 837, 879, 897, 1411, 1597, 1821
Bst6I CTCTTC 2 cut(s) 1689, 1695
BstACI GRCGYC 3 cut(s) 39, 62, 345
BstAFI CTTAAG 1 cut(s) 1679
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 8 cut(s) 47, 147, 204, 230, 349, 618, 681, 2027
BstDEI CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1953, 2008
BstDSI CCRYGG 3 cut(s) 107, 886, 1244
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 3 cut(s) 385, 514, 1669
BstFNI CGCG 1 cut(s) 242
BstH2I RGCGCY 4 cut(s) 272, 348, 1111, 1527
BstHHI GCGC 5 cut(s) 7, 271, 347, 1110, 1526
BstMAI GTCTC 3 cut(s) 27, 226, 415
BstMCI CGRYCG 2 cut(s) 207, 255
BstNI CCWGG 1 cut(s) 1190
BstNSI RCATGY 1 cut(s) 49
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 5 cut(s) 171, 191, 246, 1188, 2041
BstSFI CTRYAG 2 cut(s) 88, 1153
BstSLI GKGCMC 4 cut(s) 173, 685, 847, 895
BstSNI TACGTA 1 cut(s) 578
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 7 cut(s) 6, 107, 292, 694, 1355, 1430, 2066
BstV2I GAAGAC 1 cut(s) 1906
BstX2I RGATCY 1 cut(s) 1423
BstXI CCANNNNNNTGG 4 cut(s) 580, 863, 1925, 1988
BstYI RGATCY 1 cut(s) 1423
BstZ17I GTATAC 1 cut(s) 628
BstZI CGGCCG 1 cut(s) 204
BsuI GTATCC 1 cut(s) 1083
BsuRI GGCC 6 cut(s) 206, 260, 444, 616, 1273, 2006
BtgI CCRYGG 3 cut(s) 107, 886, 1244
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 3 cut(s) 385, 514, 1669
BtsIMutI CAGTG 5 cut(s) 167, 842, 1764, 1826, 1874
BveI ACCTGC 5 cut(s) 95, 104, 769, 1246, 1775
Cac8I GCNNGC 8 cut(s) 47, 147, 204, 230, 349, 618, 681, 2027
CaiI CAGNNNCTG 2 cut(s) 96, 307
CciI TCATGA 1 cut(s) 1464
CfoI GCGC 5 cut(s) 7, 271, 347, 1110, 1526
Cfr10I RCCGGY 3 cut(s) 202, 347, 1206
Cfr13I GGNCC 5 cut(s) 76, 93, 614, 752, 2039
CseI GACGC 3 cut(s) 28, 70, 88
Csp6I GTAC 4 cut(s) 495, 919, 1079, 1141
CviAII CATG 9 cut(s) 46, 438, 857, 887, 938, 1245, 1262, 1344, 1465
CviQI GTAC 4 cut(s) 495, 919, 1079, 1141
DdeI CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1953, 2008
DinI GGCGCC 1 cut(s) 346
DraIII CACNNNGTG 3 cut(s) 108, 440, 1490
EaeI YGGCCR 2 cut(s) 204, 442
EagI CGGCCG 1 cut(s) 204
Eam1104I CTCTTC 2 cut(s) 1689, 1695
EarI CTCTTC 2 cut(s) 1689, 1695
EciI GGCGGA 1 cut(s) 205
EclXI CGGCCG 1 cut(s) 204
Eco105I TACGTA 1 cut(s) 578
Eco130I CCWWGG 4 cut(s) 787, 886, 1244, 1641
Eco24I GRGCYC 1 cut(s) 1937
Eco31I GGTCTC 1 cut(s) 415
Eco47I GGWCC 4 cut(s) 76, 93, 752, 2039
Eco47III AGCGCT 1 cut(s) 1109
Eco52I CGGCCG 1 cut(s) 204
Eco57I CTGAAG 3 cut(s) 1322, 1740, 2103
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoO109I RGGNCCY 2 cut(s) 93, 614
EcoRI GAATTC 1 cut(s) 1807
EcoRII CCWGG 1 cut(s) 1188
EcoT14I CCWWGG 4 cut(s) 787, 886, 1244, 1641
EcoT38I GRGCYC 1 cut(s) 1937
EgeI GGCGCC 1 cut(s) 346
EheI GGCGCC 1 cut(s) 346
ErhI CCWWGG 4 cut(s) 787, 886, 1244, 1641
Esp3I CGTCTC 2 cut(s) 27, 226
FaeI CATG 9 cut(s) 49, 441, 860, 890, 941, 1248, 1265, 1347, 1468
FaqI GGGAC 4 cut(s) 115, 120, 1361, 1824
FatI CATG 9 cut(s) 45, 437, 856, 886, 937, 1244, 1261, 1343, 1464
FauI CCCGC 4 cut(s) 308, 554, 672, 1525
FauNDI CATATG 2 cut(s) 601, 1117
FbaI TGATCA 2 cut(s) 742, 1432
FblI GTMKAC 2 cut(s) 627, 832
FokI GGATG 3 cut(s) 392, 501, 1676
FriOI GRGCYC 1 cut(s) 1937
FspBI CTAG 3 cut(s) 1275, 1637, 2064
GlaI GCGC 5 cut(s) 6, 270, 346, 1109, 1525
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 4 cut(s) 272, 348, 1111, 1527
HaeIII GGCC 6 cut(s) 206, 260, 444, 616, 1273, 2006
HgaI GACGC 3 cut(s) 28, 70, 88
HhaI GCGC 5 cut(s) 7, 271, 347, 1110, 1526
Hin1I GRCGYC 3 cut(s) 39, 62, 345
Hin1II CATG 9 cut(s) 49, 441, 860, 890, 941, 1248, 1265, 1347, 1468
Hin6I GCGC 5 cut(s) 5, 269, 345, 1108, 1524
HinP1I GCGC 5 cut(s) 5, 269, 345, 1108, 1524
HincII GTYRAC 2 cut(s) 542, 833
HindII GTYRAC 2 cut(s) 542, 833
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 6 cut(s) 140, 1149, 1185, 1673, 1712, 1837
HphI GGTGA 4 cut(s) 76, 1380, 1657, 1929
Hpy166II GTNNAC 7 cut(s) 364, 542, 628, 833, 845, 893, 1648
Hpy188I TCNGA 6 cut(s) 641, 1416, 1582, 1614, 1750, 2140
Hpy188III TCNNGA 7 cut(s) 137, 469, 776, 912, 1465, 1829, 1918
Hpy8I GTNNAC 7 cut(s) 364, 542, 628, 833, 845, 893, 1648
Hpy99I CGWCG 4 cut(s) 32, 64, 70, 371
HpyAV CCTTC 9 cut(s) 280, 313, 335, 349, 534, 966, 1514, 1578, 2071
HpyCH4III ACNGT 7 cut(s) 179, 837, 879, 897, 1411, 1597, 1821
HpyCH4IV ACGT 3 cut(s) 9, 114, 577
HpyF3I CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1953, 2008
HpySE526I ACGT 3 cut(s) 9, 114, 577
Hsp92I GRCGYC 3 cut(s) 39, 62, 345
Hsp92II CATG 9 cut(s) 49, 441, 860, 890, 941, 1248, 1265, 1347, 1468
HspAI GCGC 5 cut(s) 5, 269, 345, 1108, 1524
KasI GGCGCC 1 cut(s) 344
KpnI GGTACC 1 cut(s) 922
KroI GCCGGC 2 cut(s) 202, 347
KroNI GCCGGC 2 cut(s) 204, 349
Ksp22I TGATCA 2 cut(s) 742, 1432
LmnI GCTCC 3 cut(s) 277, 304, 1115
Lsp1109I GCAGC 7 cut(s) 6, 107, 292, 694, 1355, 1430, 2066
LweI GCATC 5 cut(s) 253, 558, 1654, 1939, 2023
MaeI CTAG 3 cut(s) 1275, 1637, 2064
MaeII ACGT 3 cut(s) 9, 114, 577
MbiI CCGCTC 2 cut(s) 315, 679
MfeI CAATTG 1 cut(s) 391
MflI RGATCY 1 cut(s) 1423
MhlI GDGCHC 7 cut(s) 173, 685, 847, 895, 1495, 1937, 2031
MlsI TGGCCA 1 cut(s) 444
MluNI TGGCCA 1 cut(s) 444
Mly113I GGCGCC 1 cut(s) 345
MlyI GAGTC 3 cut(s) 1158, 1682, 1846
MmeI TCCRAC 2 cut(s) 460, 1151
Mox20I TGGCCA 1 cut(s) 444
MroNI GCCGGC 2 cut(s) 202, 347
MroXI GAANNNNTTC 1 cut(s) 464
MscI TGGCCA 1 cut(s) 444
MseI TTAA 4 cut(s) 1197, 1658, 1680, 1791
MslI CAYNNNNRTG 1 cut(s) 578
Msp20I TGGCCA 1 cut(s) 444
MspA1I CMGCKG 5 cut(s) 19, 120, 740, 1718, 2003
MspCI CTTAAG 1 cut(s) 1679
MspR9I CCNGG 5 cut(s) 173, 193, 248, 1190, 2043
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 1 cut(s) 812
MvaI CCWGG 1 cut(s) 1190
MvnI CGCG 1 cut(s) 242
NaeI GCCGGC 2 cut(s) 204, 349
NarI GGCGCC 1 cut(s) 345
NciI CCSGG 4 cut(s) 173, 193, 248, 2043
NcoI CCATGG 2 cut(s) 886, 1244
NdeI CATATG 2 cut(s) 601, 1117
NgoMIV GCCGGC 2 cut(s) 202, 347
NlaIII CATG 9 cut(s) 49, 441, 860, 890, 941, 1248, 1265, 1347, 1468
NlaIV GGNNCC 4 cut(s) 346, 615, 920, 2110
NmuCI GTSAC 6 cut(s) 100, 727, 1226, 1386, 1674, 1683
NspI RCATGY 1 cut(s) 49
PaeI GCATGC 1 cut(s) 49
PagI TCATGA 1 cut(s) 1464
PaqCI CACCTGC 2 cut(s) 95, 1775
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 812
PdiI GCCGGC 2 cut(s) 204, 349
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 3 cut(s) 140, 1185, 1712
PflFI GACNNNGTC 2 cut(s) 38, 64
PfoI TCCNGGA 1 cut(s) 191
Ple19I CGATCG 1 cut(s) 255
PleI GAGTC 3 cut(s) 1157, 1681, 1845
PluTI GGCGCC 1 cut(s) 348
PpsI GAGTC 3 cut(s) 1157, 1681, 1845
Ppu21I YACGTR 1 cut(s) 578
PpuMI RGGWCCY 1 cut(s) 93
PshAI GACNNNNGTC 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 1188
PspFI CCCAGC 1 cut(s) 810
PspGI CCWGG 1 cut(s) 1188
PspN4I GGNNCC 4 cut(s) 346, 615, 920, 2110
PspPI GGNCC 5 cut(s) 76, 93, 614, 752, 2039
PspPPI RGGWCCY 1 cut(s) 93
PstI CTGCAG 1 cut(s) 92
PstNI CAGNNNCTG 2 cut(s) 96, 307
PsuI RGATCY 1 cut(s) 1423
PsyI GACNNNGTC 2 cut(s) 38, 64
PvuI CGATCG 1 cut(s) 255
PvuII CAGCTG 4 cut(s) 19, 120, 740, 1718
RsaI GTAC 4 cut(s) 496, 920, 1080, 1142
RsaNI GTAC 4 cut(s) 495, 919, 1079, 1141
RseI CAYNNNNRTG 1 cut(s) 578
SalI GTCGAC 1 cut(s) 831
SaqAI TTAA 4 cut(s) 1197, 1658, 1680, 1791
Sau96I GGNCC 5 cut(s) 76, 93, 614, 752, 2039
SbfI CCTGCAGG 1 cut(s) 92
SchI GAGTC 3 cut(s) 1158, 1682, 1846
ScrFI CCNGG 5 cut(s) 173, 193, 248, 1190, 2043
SdaI CCTGCAGG 1 cut(s) 92
SduI GDGCHC 7 cut(s) 173, 685, 847, 895, 1495, 1937, 2031
SfaNI GCATC 5 cut(s) 253, 558, 1654, 1939, 2023
SfcI CTRYAG 2 cut(s) 88, 1153
SfoI GGCGCC 1 cut(s) 346
SinI GGWCC 4 cut(s) 76, 93, 752, 2039
SmiMI CAYNNNNRTG 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 1679
SmoI CTYRAG 1 cut(s) 1679
SnaBI TACGTA 1 cut(s) 578
SphI GCATGC 1 cut(s) 49
Sse8387I CCTGCAGG 1 cut(s) 92
SspDI GGCGCC 1 cut(s) 344
SspMI CTAG 3 cut(s) 1275, 1637, 2064
StyD4I CCNGG 5 cut(s) 171, 191, 246, 1188, 2041
StyI CCWWGG 4 cut(s) 787, 886, 1244, 1641
TaaI ACNGT 7 cut(s) 179, 837, 879, 897, 1411, 1597, 1821
TaiI ACGT 3 cut(s) 12, 117, 580
TaqI TCGA 2 cut(s) 255, 832
TauI GCSGC 5 cut(s) 242, 269, 513, 2006, 2025
TfiI GAWTC 3 cut(s) 140, 1185, 1712
Tru1I TTAA 4 cut(s) 1197, 1658, 1680, 1791
Tru9I TTAA 4 cut(s) 1197, 1658, 1680, 1791
TscAI CASTG 5 cut(s) 167, 842, 1771, 1826, 1881
TseFI GTSAC 6 cut(s) 100, 727, 1226, 1386, 1674, 1683
TseI GCWGC 7 cut(s) 19, 120, 280, 707, 1368, 1443, 2054
Tsp45I GTSAC 6 cut(s) 100, 727, 1226, 1386, 1674, 1683
TspDTI ATGAA 7 cut(s) 1248, 1272, 1560, 1565, 1707, 1770, 1980
TspGWI ACGGA 2 cut(s) 45, 290
TspRI CASTG 5 cut(s) 167, 842, 1771, 1826, 1881
Tth111I GACNNNGTC 2 cut(s) 38, 64
Vha464I CTTAAG 1 cut(s) 1679
VneI GTGCAC 2 cut(s) 843, 891
VpaK11BI GGWCC 4 cut(s) 76, 93, 752, 2039
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 2 cut(s) 1796, 1807
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmiI GTMKAC 2 cut(s) 627, 832
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 3 cut(s) 1275, 1637, 2064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.