Rmu_sc0002393.1_g000019

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002393.1
OG0000644
Physical Location & Seq
Reverse (-)
87159 .. 88912
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002393.1_g000019.1.cds

Sequence Viewer

Length: 636 bp
atgcgcctacggaggtgggactgcgatatgatggggtttacaggattgaaaaatgttggcggaaagatggaacacagtgatgatcatggggaccgtccaaggcctctgccagatattgcagagttgaacagtgccattgatataacaaatacaaagggttctccttcttgggactatgatgcagaaaaagcgtgctggatgtggaaaaagcctcgaccacttagcagacaactggtgcataggggagaaccagatgggaagaaaattaggagagttagacggcaggaacagaatgcgaaagaaaaagttctcacagaattactccagcagaaagaggaagatgttgcagaggaagtggaagacgctgaggaagatgaagatgatgaagaagaggaagaagaggaagaagctgaagaaacttacaatgccggtgaagagagtgaagccgaagacaaaggaagcatcccaccaatacctcagaccgaaactacagagcaacaaacctgtgagcaaaagaaaccaagtcataagcgaaataggtcgagcaatggagacaacgtgttggatggcggagcactgaccaagagaagcaagaggactaaagtgggagcagctagtagtctgattgattcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

211

Amino Acids

24.01

Weight (kDa)

4.78

Isoelectric Point (pI)

79.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 60, 570
AcuI CTGAAG 1 cut(s) 432
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 2 cut(s) 49, 127
AluBI AGCT 2 cut(s) 410, 614
AluI AGCT 2 cut(s) 410, 614
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 1 cut(s) 546
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 1 cut(s) 611
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 443
AvaII GGWCC 1 cut(s) 91
BbsI GAAGAC 2 cut(s) 366, 456
Bbv12I GWGCWC 1 cut(s) 577
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 623
BccI CCATC 4 cut(s) 25, 61, 248, 560
BceAI ACGGC 1 cut(s) 296
BclI TGATCA 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 546
BfaI CTAG 1 cut(s) 615
BfmI CTRYAG 1 cut(s) 489
BisI GCNGC 1 cut(s) 612
BlsI GCNGC 1 cut(s) 613
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 92
BmsI GCATC 2 cut(s) 169, 471
BpiI GAAGAC 2 cut(s) 366, 456
BpmI CTGGAG 1 cut(s) 308
Bpu10I CCTNAGC 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 553
BseDI CCNNGG 1 cut(s) 98
BseGI GGATG 3 cut(s) 204, 462, 571
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 2 cut(s) 357, 491
BseNI ACTGG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 623
BshFI GGCC 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 1 cut(s) 429
BslFI GGGAC 3 cut(s) 32, 104, 185
BsmAI GTCTC 1 cut(s) 546
BsmFI GGGAC 3 cut(s) 32, 104, 185
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 2 cut(s) 60, 570
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 2 cut(s) 358, 490
BspHI TCATGA 1 cut(s) 632
BspLI GGNNCC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 553
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 1 cut(s) 237
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 1 cut(s) 98
BssMI GATC 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 98
Bst4CI ACNGT 3 cut(s) 77, 95, 131
Bst6I CTCTTC 3 cut(s) 384, 393, 429
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 3 cut(s) 221, 366, 477
BstF5I GGATG 3 cut(s) 204, 462, 571
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 546
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 1 cut(s) 623
BstV2I GAAGAC 2 cut(s) 366, 456
BsuRI GGCC 1 cut(s) 103
BtsCI GGATG 3 cut(s) 204, 462, 571
BtsIMutI CAGTG 3 cut(s) 82, 136, 575
Cac8I GCNNGC 1 cut(s) 193
CciI TCATGA 1 cut(s) 632
CfoI GCGC 1 cut(s) 6
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 1 cut(s) 91
CseI GACGC 1 cut(s) 371
CviAII CATG 2 cut(s) 86, 633
CviJI RGCY 5 cut(s) 103, 211, 410, 446, 614
CviKI_1 RGCY 5 cut(s) 103, 211, 410, 446, 614
DdeI CTNAG 3 cut(s) 221, 366, 477
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
Eam1104I CTCTTC 3 cut(s) 384, 393, 429
EarI CTCTTC 3 cut(s) 384, 393, 429
EciI GGCGGA 2 cut(s) 75, 585
Eco130I CCWWGG 1 cut(s) 98
Eco147I AGGCCT 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 432
EcoT14I CCWWGG 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 2 cut(s) 89, 636
FaiI YATR 7 cut(s) 29, 87, 143, 177, 240, 528, 634
FaqI GGGAC 3 cut(s) 32, 104, 185
FatI CATG 2 cut(s) 85, 632
FbaI TGATCA 1 cut(s) 82
Fnu4HI GCNGC 1 cut(s) 612
FokI GGATG 3 cut(s) 211, 449, 578
Fsp4HI GCNGC 1 cut(s) 612
FspBI CTAG 1 cut(s) 615
GlaI GCGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 612
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 1 cut(s) 103
HapII CCGG 1 cut(s) 429
HgaI GACGC 1 cut(s) 371
HhaI GCGC 1 cut(s) 6
Hin1II CATG 2 cut(s) 89, 636
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HinfI GANTC 1 cut(s) 629
HpaII CCGG 1 cut(s) 429
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 1 cut(s) 39
Hpy188I TCNGA 2 cut(s) 480, 624
Hpy188III TCNNGA 1 cut(s) 633
Hpy8I GTNNAC 1 cut(s) 39
HpyAV CCTTC 1 cut(s) 174
HpyCH4III ACNGT 3 cut(s) 77, 95, 131
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 4 cut(s) 119, 182, 238, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 3 cut(s) 221, 366, 477
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 2 cut(s) 89, 636
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 572, 608
LpnPI CCDG 9 cut(s) 27, 123, 181, 218, 264, 269, 338, 442, 517
Lsp1109I GCAGC 1 cut(s) 623
LweI GCATC 2 cut(s) 169, 471
MaeI CTAG 1 cut(s) 615
MaeII ACGT 1 cut(s) 558
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 2 cut(s) 264, 317
MmeI TCCRAC 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 306
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 1 cut(s) 429
Mva1269I GAATGC 1 cut(s) 298
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeII GATC 1 cut(s) 82
NlaIII CATG 2 cut(s) 89, 636
NlaIV GGNNCC 1 cut(s) 92
PagI TCATGA 1 cut(s) 632
PceI AGGCCT 1 cut(s) 103
PctI GAATGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 629
PkrI GCNGC 1 cut(s) 613
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 78
SatI GCNGC 1 cut(s) 612
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 577
SetI ASST 7 cut(s) 17, 412, 478, 506, 542, 561, 616
SfaNI GCATC 2 cut(s) 169, 471
SfcI CTRYAG 1 cut(s) 489
SinI GGWCC 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 2 cut(s) 264, 317
SseBI AGGCCT 1 cut(s) 103
SsiI CCGC 2 cut(s) 60, 570
SspMI CTAG 1 cut(s) 615
StuI AGGCCT 1 cut(s) 103
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 3 cut(s) 77, 95, 131
TaiI ACGT 1 cut(s) 561
TaqI TCGA 2 cut(s) 214, 542
TaqII GACCGA 1 cut(s) 497
TasI AATT 2 cut(s) 264, 317
TfiI GAWTC 1 cut(s) 629
TscAI CASTG 3 cut(s) 82, 136, 582
TseI GCWGC 1 cut(s) 611
TspDTI ATGAA 3 cut(s) 390, 399, 621
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 3 cut(s) 82, 136, 582
VpaK11BI GGWCC 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.