Rorug06G0491700

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000644
Physical Location & Seq
Forward (+)
60982195 .. 60986682
4488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0491700.1

Sequence Viewer

Length: 2127 bp
ATGAAGAGAGGAGGCAGAGATGATGAAAAGGTTATGGGGCCTATGTTCCCAAGACTCCATGTGAATGATGCAGCAGATAAAGGAGGGCCAAGAGCACCTCCAAGGAACAAGATGGCCCTGTATGAACAGCTTAGTATACCCTCTCAGAGGTTCAACCATGGGGTTATGCCACTCAATCAAAACAGTATGGGATCCCCAGCTTTCTCCAATCAGGGGAATGGTCCTGAAAGAAATTTGCCTTTACCAATTCATGGTCGTCCATCTACACCTACTCATCACTCTGGCGGCCAGTCTGATGGAGTAAATGTGAACATGCCCTCAGTACAACCTCGGCAAAGAAAGAGGGCAGGAGATGAAGATGATTTTGCAGTTCCTGTATTTGTTCAATCAAGGATGGGTCAATGTCAGAGTAGAACTCAAAATGGTGGTGGTAATGCAAAACTTACTCCCTTCAGCCAGTCTCATTCTGACTGTGCAATAAAATTCAAAAGTGCATCCAAGAAGGATAATAAACAAAGTAGCTCCCCCAGTCTCAATTTAAGACGAGAATTTAGAAGTGAGACAGAAGAGGATCTCAACGCATGTAGCTCAGCTAGGGACCATTCAGTAGAAGCTGCCACAAATATCTCAACTAGAGAAATGGTTGATGTTCCTGTAAATGTGAATACTTCTCCAAATAAAGAGTATGAAGACCATCCTGTTCCTACTTTCAACAGGTCTTGTGATAGTGATGCTTGCTTACAACAAAACTCTAGATCTGGGTCCCAACTAAACAACACTGAACAAGATGATAGTCTCCTTGAGTCCACAAGGGATGTAGAGAAGGGAACTGTTTCACAGGCAAGAAGTGTTTCTCGTTACGGGGGGAATCATAGCAGCCCCAACGAGCCTGATAATGACAGTGAATACCATGTAGACAGAATGTGTATTTCAGCACAAATGGGAAATGCAGACAAAGGTGATGATGTCTCAGAGACCTCCATGGTGGATTCTATATCAGGCATGGACATCTCTCCTGATGATGTAGTAGGAATAATAGGTCAGAAGCATTTCTGGAAGGCAAGAAGGGCAATTGTCAATCAGCAAAGATTGTTTGCAGTGCAAGTATTTGAGTTGCATAGACTCATTAAGGTCCAACGACTGATTGCTGGATCTCCACATCTTTTGCTTGAAGATACTGCTTTCCTAGGCACATCCTTAAGGGGTTCTCCTGCAAAGAAACTCCCGGCAGAGTATGTTGTAAAGCCACTACTACTAAAGCGCAAGCATGAGTCTGAGAAGTCAAATAACAAAATGGAATGTTCTGCTGAAAATGCGGTTGGGAAAGCTTCTCTTTCTTCTGTGAAAAATGGTAGCCAGCCTTCACATTATGGACCATATGTAGGGAGTCCACCTCCTCCAGCACCTGTGCCTGCTGACAATAAAGCAGCTCCTTGGTGCTTCCATCAACCACCTGGGCATCAGTATTTAATTCCTGTTATGTCTCCTTCTGAAGGACTCATATACAAGCCATATAATGGACCTGGATTTGTGGGAGCAGCTTGTGGAGGATGTGGACCTTTTGGCTCAAGTTCCATGCCGGGTAACTTCATTAAGCCAGCATATGGGGTTCCGGGATCTCATCATCAAGGAATGGGGGTTCTCCCTGTCCCTCATCCTACTTACTTTCCTCCCTATGGCATGTCCGTCATGAATCCCACCATGTCAAGCTCAGCTGTTGAACAAATGAATTGGTTTGCTGGACCTGGTATGCATGGATCAACTGATCAGTTGTCAGGAAACTCTAATTTGCAGCATCAAAGCTCATGTAATATGCCAACACCCAAAAATGGAGCTATTCCTCATGCCATGAAGTCTCGAGCATCAAACCCCAGTGAGTTACAAGGAAGTACTGGTAATAGTCCCGGTGACAGAGCACCAGTTGCGACGGATCAAAATGCTGATGAAAGTGATGCACTTCAACTTTTCCCTTCTGTTCCAGCAGTTCCAGAGCGAGTTCCCCAACCTCATGATACAAACCAGGCAACACAAGTGATAAGAGTTGTCCCGCACAATCCTAGATCTGCTACTGCATCAGCTGCTCGAATTTTCCAATCTATACAAGAAGAGAGAAAACAGTATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

708

Amino Acids

76.57

Weight (kDa)

7.36

Isoelectric Point (pI)

63.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 251, 1517, 1604
AccI GTMKAC 2 cut(s) 136, 915
AciI CCGC 3 cut(s) 285, 1316, 2048
AclWI GGATC 7 cut(s) 186, 199, 579, 1159, 1624, 1765, 1938
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 4 cut(s) 232, 482, 548, 2085
AcuI CTGAAG 2 cut(s) 436, 1512
AfaI GTAC 2 cut(s) 324, 1891
AfiI CCNNNNNNNGG 9 cut(s) 147, 213, 251, 1382, 1493, 1517, 1604, 1676, 1829
AflII CTTAAG 1 cut(s) 1198
AgsI TTSAA 7 cut(s) 154, 386, 487, 712, 1172, 1721, 1961
AjnI CCWGG 4 cut(s) 1453, 1522, 1744, 2019
AjuI GAANNNNNNNTTGG 2 cut(s) 1302, 1334
AloI GAACNNNNNNTCC 2 cut(s) 1623, 1655
Alw21I GWGCWC 2 cut(s) 97, 1918
Alw26I GTCTC 8 cut(s) 465, 536, 554, 800, 968, 973, 1488, 1860
AlwI GGATC 7 cut(s) 186, 199, 579, 1159, 1624, 1765, 1938
AlwNI CAGNNNCTG 2 cut(s) 374, 1406
Ama87I CYCGRG 1 cut(s) 1857
AoxI GGCC 4 cut(s) 38, 86, 114, 286
ApeKI GCWGC 7 cut(s) 71, 614, 876, 1427, 1538, 1792, 2078
ApoI RAATTY 4 cut(s) 232, 482, 548, 2085
Asp700I GAANNNNTTC 3 cut(s) 832, 850, 1049
AspA2I CCTAGG 1 cut(s) 1186
AspLEI GCGC 1 cut(s) 1263
AsuC2I CCSGG 4 cut(s) 1226, 1581, 1614, 1905
AsuHPI GGTGA 2 cut(s) 971, 1919
AvaI CYCGRG 1 cut(s) 1857
AvaII GGWCC 8 cut(s) 221, 598, 762, 1132, 1373, 1520, 1556, 1742
AvrII CCTAGG 1 cut(s) 1186
BamHI GGATCC 1 cut(s) 191
BarI GAAGNNNNNNTAC 2 cut(s) 1345, 1377
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 2 cut(s) 97, 1918
BbvI GCAGC 7 cut(s) 83, 601, 888, 1439, 1550, 1804, 2065
BccI CCATC 6 cut(s) 106, 268, 290, 388, 702, 1452
BciT130I CCWGG 4 cut(s) 1455, 1524, 1746, 2021
BclI TGATCA 1 cut(s) 1765
BcnI CCSGG 4 cut(s) 1226, 1581, 1614, 1905
BcoDI GTCTC 8 cut(s) 465, 536, 554, 800, 968, 973, 1488, 1860
BfaI CTAG 5 cut(s) 594, 633, 753, 1187, 2058
BfrI CTTAAG 1 cut(s) 1198
BglII AGATCT 2 cut(s) 755, 2060
BisI GCNGC 8 cut(s) 72, 286, 615, 877, 1428, 1539, 1793, 2079
BlnI CCTAGG 1 cut(s) 1186
BlpI GCTNAGC 2 cut(s) 589, 1711
BlsI GCNGC 8 cut(s) 73, 287, 616, 878, 1429, 1540, 1794, 2080
BmcAI AGTACT 1 cut(s) 1891
Bme1390I CCNGG 8 cut(s) 1226, 1455, 1524, 1581, 1614, 1746, 1905, 2021
Bme18I GGWCC 8 cut(s) 221, 598, 762, 1132, 1373, 1520, 1556, 1742
BmeT110I CYCGRG 1 cut(s) 1857
BmiI GGNNCC 6 cut(s) 39, 193, 599, 763, 764, 1611
BmrFI CCNGG 8 cut(s) 1226, 1455, 1524, 1581, 1614, 1746, 1905, 2021
BmrI ACTGGG 2 cut(s) 522, 1866
BmsI GCATC 8 cut(s) 58, 503, 721, 1468, 1804, 1871, 1942, 2081
BmuI ACTGGG 2 cut(s) 522, 1866
BpiI GAAGAC 1 cut(s) 696
BplI GAGNNNNNCTC 4 cut(s) 400, 432, 1378, 1410
BpmI CTGGAG 1 cut(s) 1383
Bpu1102I GCTNAGC 2 cut(s) 589, 1711
BpuEI CTTGAG 2 cut(s) 821, 1552
BpuMI CCSGG 4 cut(s) 1226, 1581, 1614, 1905
BsaI GGTCTC 1 cut(s) 968
BsaJI CCNNGG 7 cut(s) 101, 157, 329, 981, 1186, 1433, 1454
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 9 cut(s) 147, 213, 251, 1382, 1493, 1517, 1604, 1676, 1829
Bse1I ACTGG 6 cut(s) 289, 457, 528, 1872, 1897, 1919
BseBI CCWGG 4 cut(s) 1455, 1524, 1746, 2021
BseDI CCNNGG 7 cut(s) 101, 157, 329, 981, 1186, 1433, 1454
BseGI GGATG 7 cut(s) 399, 494, 694, 820, 1193, 1556, 1654
BseLI CCNNNNNNNGG 9 cut(s) 147, 213, 251, 1382, 1493, 1517, 1604, 1676, 1829
BseMII CTCAG 6 cut(s) 158, 333, 603, 984, 1266, 1725
BseNI ACTGG 6 cut(s) 289, 457, 528, 1872, 1897, 1919
BseRI GAGGAG 2 cut(s) 24, 1386
BseXI GCAGC 7 cut(s) 83, 601, 888, 1439, 1550, 1804, 2065
BseYI CCCAGC 1 cut(s) 196
BshFI GGCC 4 cut(s) 40, 88, 116, 288
BsiHKAI GWGCWC 2 cut(s) 97, 1918
BsiHKCI CYCGRG 1 cut(s) 1857
BsiSI CCGG 4 cut(s) 1226, 1580, 1613, 1905
BslFI GGGAC 5 cut(s) 611, 748, 1634, 1887, 2030
BslI CCNNNNNNNGG 9 cut(s) 147, 213, 251, 1382, 1493, 1517, 1604, 1676, 1829
BsmAI GTCTC 8 cut(s) 465, 536, 554, 800, 968, 973, 1488, 1860
BsmFI GGGAC 5 cut(s) 611, 748, 1634, 1887, 2030
BsnI GGCC 4 cut(s) 40, 88, 116, 288
Bso31I GGTCTC 1 cut(s) 968
BsoBI CYCGRG 1 cut(s) 1857
Bsp1286I GDGCHC 2 cut(s) 97, 1918
Bsp143I GATC 9 cut(s) 191, 571, 755, 1151, 1616, 1757, 1765, 1930, 2060
Bsp1720I GCTNAGC 2 cut(s) 589, 1711
Bsp19I CCATGG 2 cut(s) 157, 981
BspACI CCGC 3 cut(s) 285, 1316, 2048
BspANI GGCC 4 cut(s) 40, 88, 116, 288
BspCNI CTCAG 6 cut(s) 157, 332, 602, 983, 1267, 1724
BspHI TCATGA 2 cut(s) 1689, 2008
BspLI GGNNCC 6 cut(s) 39, 193, 599, 763, 764, 1611
BspPI GGATC 7 cut(s) 186, 199, 579, 1159, 1624, 1765, 1938
BspTI CTTAAG 1 cut(s) 1198
BspTNI GGTCTC 1 cut(s) 968
BsrI ACTGG 6 cut(s) 289, 457, 528, 1872, 1897, 1919
BssECI CCNNGG 7 cut(s) 101, 157, 329, 981, 1186, 1433, 1454
BssMI GATC 9 cut(s) 191, 571, 755, 1151, 1616, 1757, 1765, 1930, 2060
BssNAI GTATAC 1 cut(s) 137
BssT1I CCWWGG 5 cut(s) 101, 157, 981, 1186, 1433
Bst1107I GTATAC 1 cut(s) 137
Bst2UI CCWGG 4 cut(s) 1455, 1524, 1746, 2021
Bst4CI ACNGT 5 cut(s) 185, 473, 832, 902, 2118
Bst6I CTCTTC 2 cut(s) 561, 2100
BstAFI CTTAAG 1 cut(s) 1198
BstAPI GCANNNNNTGC 2 cut(s) 1922, 2078
BstC8I GCNNGC 5 cut(s) 736, 1265, 1358, 1413, 1599
BstDEI CTNAG 7 cut(s) 131, 144, 319, 589, 970, 1275, 1711
BstDSI CCRYGG 2 cut(s) 157, 981
BstENI CCTNNNNNAGG 2 cut(s) 145, 1491
BstF5I GGATG 7 cut(s) 399, 494, 694, 820, 1193, 1556, 1654
BstHHI GCGC 1 cut(s) 1263
BstKTI GATC 9 cut(s) 194, 574, 758, 1154, 1619, 1760, 1768, 1933, 2063
BstMAI GTCTC 8 cut(s) 465, 536, 554, 800, 968, 973, 1488, 1860
BstMBI GATC 9 cut(s) 191, 571, 755, 1151, 1616, 1757, 1765, 1930, 2060
BstMWI GCNNNNNNNGC 4 cut(s) 1067, 1313, 1922, 2078
BstNI CCWGG 4 cut(s) 1455, 1524, 1746, 2021
BstNSI RCATGY 3 cut(s) 316, 585, 1684
BstSCI CCNGG 8 cut(s) 1224, 1453, 1522, 1579, 1612, 1744, 1903, 2019
BstV1I GCAGC 7 cut(s) 83, 601, 888, 1439, 1550, 1804, 2065
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 6 cut(s) 191, 571, 755, 1151, 1616, 2060
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 6 cut(s) 191, 571, 755, 1151, 1616, 2060
BstZ17I GTATAC 1 cut(s) 137
BsuRI GGCC 4 cut(s) 40, 88, 116, 288
BtgI CCRYGG 2 cut(s) 157, 981
BtsCI GGATG 7 cut(s) 399, 494, 694, 820, 1193, 1556, 1654
BtsI GCAGTG 1 cut(s) 1104
BtsIMutI CAGTG 4 cut(s) 777, 907, 1104, 1879
Cac8I GCNNGC 5 cut(s) 736, 1265, 1358, 1413, 1599
CaiI CAGNNNCTG 2 cut(s) 374, 1406
CciI TCATGA 2 cut(s) 1689, 2008
CfoI GCGC 1 cut(s) 1263
CsiI ACCWGGT 1 cut(s) 1744
Csp6I GTAC 2 cut(s) 323, 1890
CspCI CAANNNNNGTGG 2 cut(s) 1146, 1181
CviQI GTAC 2 cut(s) 323, 1890
DdeI CTNAG 7 cut(s) 131, 144, 319, 589, 970, 1275, 1711
DpnI GATC 9 cut(s) 193, 573, 757, 1153, 1618, 1759, 1767, 1932, 2062
DpnII GATC 9 cut(s) 191, 571, 755, 1151, 1616, 1757, 1765, 1930, 2060
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 561, 2100
EarI CTCTTC 2 cut(s) 561, 2100
Eco130I CCWWGG 5 cut(s) 101, 157, 981, 1186, 1433
Eco31I GGTCTC 1 cut(s) 968
Eco47I GGWCC 8 cut(s) 221, 598, 762, 1132, 1373, 1520, 1556, 1742
Eco57I CTGAAG 2 cut(s) 436, 1512
Eco88I CYCGRG 1 cut(s) 1857
EcoNI CCTNNNNNAGG 2 cut(s) 145, 1491
EcoO109I RGGNCCY 2 cut(s) 38, 762
EcoRII CCWGG 4 cut(s) 1453, 1522, 1744, 2019
EcoT14I CCWWGG 5 cut(s) 101, 157, 981, 1186, 1433
EcoT22I ATGCAT 1 cut(s) 1755
ErhI CCWWGG 5 cut(s) 101, 157, 981, 1186, 1433
FalI AAGNNNNNCTT 2 cut(s) 1317, 1349
FaqI GGGAC 5 cut(s) 611, 748, 1634, 1887, 2030
FauI CCCGC 1 cut(s) 2055
FauNDI CATATG 2 cut(s) 1378, 1603
FbaI TGATCA 1 cut(s) 1765
FblI GTMKAC 2 cut(s) 136, 915
Fnu4HI GCNGC 8 cut(s) 72, 286, 615, 877, 1428, 1539, 1793, 2079
FokI GGATG 7 cut(s) 406, 481, 681, 827, 1180, 1563, 1641
Fsp4HI GCNGC 8 cut(s) 72, 286, 615, 877, 1428, 1539, 1793, 2079
FspBI CTAG 5 cut(s) 594, 633, 753, 1187, 2058
GlaI GCGC 1 cut(s) 1262
GluI GCNGC 8 cut(s) 72, 286, 615, 877, 1428, 1539, 1793, 2079
GsaI CCCAGC 1 cut(s) 200
GsuI CTGGAG 1 cut(s) 1383
HaeIII GGCC 4 cut(s) 40, 88, 116, 288
HapII CCGG 4 cut(s) 1226, 1580, 1613, 1905
HhaI GCGC 1 cut(s) 1263
Hin6I GCGC 1 cut(s) 1261
HinP1I GCGC 1 cut(s) 1261
HindIII AAGCTT 1 cut(s) 1326
HinfI GANTC 9 cut(s) 54, 803, 868, 989, 1122, 1271, 1387, 1497, 1693
HpaII CCGG 4 cut(s) 1226, 1580, 1613, 1905
HphI GGTGA 2 cut(s) 971, 1919
Hpy166II GTNNAC 6 cut(s) 137, 310, 807, 916, 1391, 1556
Hpy188I TCNGA 8 cut(s) 147, 295, 408, 469, 973, 1044, 1276, 1492
Hpy188III TCNNGA 9 cut(s) 224, 753, 1016, 1054, 1690, 1776, 1857, 1988, 2009
Hpy8I GTNNAC 6 cut(s) 137, 310, 807, 916, 1391, 1556
Hpy99I CGWCG 1 cut(s) 1930
HpyAV CCTTC 9 cut(s) 460, 496, 817, 1051, 1059, 1371, 1487, 1497, 1980
HpyCH4III ACNGT 5 cut(s) 185, 473, 832, 902, 2118
HpyF10VI GCNNNNNNNGC 4 cut(s) 1067, 1313, 1922, 2078
HpyF3I CTNAG 7 cut(s) 131, 144, 319, 589, 970, 1275, 1711
HspAI GCGC 1 cut(s) 1261
KflI GGGWCCC 1 cut(s) 762
Ksp22I TGATCA 1 cut(s) 1765
Kzo9I GATC 9 cut(s) 191, 571, 755, 1151, 1616, 1757, 1765, 1930, 2060
LmnI GCTCC 4 cut(s) 527, 1435, 1535, 1832
Lsp1109I GCAGC 7 cut(s) 83, 601, 888, 1439, 1550, 1804, 2065
LweI GCATC 8 cut(s) 58, 503, 721, 1468, 1804, 1871, 1942, 2081
MabI ACCWGGT 1 cut(s) 1744
MaeI CTAG 5 cut(s) 594, 633, 753, 1187, 2058
MaeIII GTNAC 4 cut(s) 857, 1583, 1878, 1907
MalI GATC 9 cut(s) 193, 573, 757, 1153, 1618, 1759, 1767, 1932, 2062
MboI GATC 9 cut(s) 191, 571, 755, 1151, 1616, 1757, 1765, 1930, 2060
MboII GAAGA 7 cut(s) 16, 368, 578, 701, 1184, 1329, 2117
MfeI CAATTG 1 cut(s) 1071
MflI RGATCY 6 cut(s) 191, 571, 755, 1151, 1616, 2060
MhlI GDGCHC 2 cut(s) 97, 1918
MlyI GAGTC 6 cut(s) 48, 812, 1116, 1280, 1396, 1491
MmeI TCCRAC 1 cut(s) 1159
Mph1103I ATGCAT 1 cut(s) 1755
MroXI GAANNNNTTC 3 cut(s) 832, 850, 1049
MseI TTAA 5 cut(s) 539, 1128, 1199, 1469, 1593
MslI CAYNNNNRTG 1 cut(s) 63
MspA1I CMGCKG 2 cut(s) 1715, 2078
MspCI CTTAAG 1 cut(s) 1198
MspI CCGG 4 cut(s) 1226, 1580, 1613, 1905
MspR9I CCNGG 8 cut(s) 1226, 1455, 1524, 1581, 1614, 1746, 1905, 2021
MunI CAATTG 1 cut(s) 1071
MvaI CCWGG 4 cut(s) 1455, 1524, 1746, 2021
MwoI GCNNNNNNNGC 4 cut(s) 1067, 1313, 1922, 2078
NciI CCSGG 4 cut(s) 1226, 1581, 1614, 1905
NcoI CCATGG 2 cut(s) 157, 981
NdeI CATATG 2 cut(s) 1378, 1603
NdeII GATC 9 cut(s) 191, 571, 755, 1151, 1616, 1757, 1765, 1930, 2060
NlaIV GGNNCC 6 cut(s) 39, 193, 599, 763, 764, 1611
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 1907
NsiI ATGCAT 1 cut(s) 1755
NspI RCATGY 3 cut(s) 316, 585, 1684
PaeR7I CTCGAG 1 cut(s) 1857
PagI TCATGA 2 cut(s) 1689, 2008
PdmI GAANNNNTTC 3 cut(s) 832, 850, 1049
PfeI GAWTC 3 cut(s) 868, 989, 1693
PflMI CCANNNNNTGG 3 cut(s) 251, 1517, 1604
PfoI TCCNGGA 1 cut(s) 1612
PkrI GCNGC 8 cut(s) 73, 287, 616, 878, 1429, 1540, 1794, 2080
PleI GAGTC 6 cut(s) 48, 811, 1116, 1279, 1395, 1491
PpsI GAGTC 6 cut(s) 48, 811, 1116, 1279, 1395, 1491
PpuMI RGGWCCY 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 762
Psp6I CCWGG 4 cut(s) 1453, 1522, 1744, 2019
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 4 cut(s) 1453, 1522, 1744, 2019
PspN4I GGNNCC 6 cut(s) 39, 193, 599, 763, 764, 1611
PspPPI RGGWCCY 1 cut(s) 762
PstNI CAGNNNCTG 2 cut(s) 374, 1406
PsuI RGATCY 6 cut(s) 191, 571, 755, 1151, 1616, 2060
PvuII CAGCTG 2 cut(s) 1715, 2078
RsaI GTAC 2 cut(s) 324, 1891
RsaNI GTAC 2 cut(s) 323, 1890
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 5 cut(s) 539, 1128, 1199, 1469, 1593
SatI GCNGC 8 cut(s) 72, 286, 615, 877, 1428, 1539, 1793, 2079
Sau3AI GATC 9 cut(s) 191, 571, 755, 1151, 1616, 1757, 1765, 1930, 2060
ScaI AGTACT 1 cut(s) 1891
SchI GAGTC 6 cut(s) 48, 812, 1116, 1280, 1396, 1491
ScrFI CCNGG 8 cut(s) 1226, 1455, 1524, 1581, 1614, 1746, 1905, 2021
SduI GDGCHC 2 cut(s) 97, 1918
SexAI ACCWGGT 1 cut(s) 1744
SfaNI GCATC 8 cut(s) 58, 503, 721, 1468, 1804, 1871, 1942, 2081
Sfr274I CTCGAG 1 cut(s) 1857
SinI GGWCC 8 cut(s) 221, 598, 762, 1132, 1373, 1520, 1556, 1742
SlaI CTCGAG 1 cut(s) 1857
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 4 cut(s) 800, 1198, 1567, 1857
SmoI CTYRAG 4 cut(s) 800, 1198, 1567, 1857
SsiI CCGC 3 cut(s) 285, 1316, 2048
SspMI CTAG 5 cut(s) 594, 633, 753, 1187, 2058
StyD4I CCNGG 8 cut(s) 1224, 1453, 1522, 1579, 1612, 1744, 1903, 2019
StyI CCWWGG 5 cut(s) 101, 157, 981, 1186, 1433
TaaI ACNGT 5 cut(s) 185, 473, 832, 902, 2118
TaqI TCGA 2 cut(s) 1858, 2083
TatI WGTACW 2 cut(s) 322, 1889
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 3 cut(s) 868, 989, 1693
Tru1I TTAA 5 cut(s) 539, 1128, 1199, 1469, 1593
Tru9I TTAA 5 cut(s) 539, 1128, 1199, 1469, 1593
TscAI CASTG 4 cut(s) 784, 907, 1104, 1879
TseFI GTSAC 1 cut(s) 1907
TseI GCWGC 7 cut(s) 71, 614, 876, 1427, 1538, 1792, 2078
Tsp45I GTSAC 1 cut(s) 1907
TspGWI ACGGA 2 cut(s) 1675, 1943
TspRI CASTG 4 cut(s) 784, 907, 1104, 1879
Van91I CCANNNNNTGG 3 cut(s) 251, 1517, 1604
Vha464I CTTAAG 1 cut(s) 1198
VpaK11BI GGWCC 8 cut(s) 221, 598, 762, 1132, 1373, 1520, 1556, 1742
XagI CCTNNNNNAGG 2 cut(s) 145, 1491
XapI RAATTY 4 cut(s) 232, 482, 548, 2085
XbaI TCTAGA 1 cut(s) 752
XceI RCATGY 3 cut(s) 316, 585, 1684
XcmI CCANNNNNNNNNTGG 1 cut(s) 1451
XhoI CTCGAG 1 cut(s) 1857
XmaJI CCTAGG 1 cut(s) 1186
XmiI GTMKAC 2 cut(s) 136, 915
XmnI GAANNNNTTC 3 cut(s) 832, 850, 1049
XspI CTAG 5 cut(s) 594, 633, 753, 1187, 2058
ZrmI AGTACT 1 cut(s) 1891
Zsp2I ATGCAT 1 cut(s) 1755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.