RchiOBHm_Chr6g0248801

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000644
Physical Location & Seq
Reverse (-)
4702946 .. 4710832
7887 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22302

Sequence Viewer

Length: 933 bp
ATGCGCCTACGGAGGTGGGACTGCGATATGATGGGGTTTACAGGATTGAAAAATGTTGGCGGAAAGATGGAACACAGGGTTAAGGTTTGCAGATATCTTTTTGTAAGATGTGACAACGAACCTGCGCCTTGGACGAGTGATGATCATGGGGACCGTCCAAGGCCTCTGCCAGATATTGCAGAGTTGAACAGTGCCATTGATATAACAAATACAAAGGGTTCTCCTTCTTGGGACTATGATGCAGAAAAAGCGTGCTGGATGTGGAAAAAGCCTCGACCACTTAGCAGACAACTGGTGGATAGGGGAGAACCAGATGGGAAGAAAATTAGGAGAGTTAGACGGCAGGAACAGAATGCGAAAGAAAAAGTTCTCAAAGGGTTTAGGTGTGATAAATGTCAGAAGATTATGACTGAACCAGTTACTACACCATGTGGTCATAACTTCTGCAAAGCTTGTCTGCAAGATACCTTTGCTGGTATAAGTTTAATAAAACAGAGGACGTGCCAAGGCGGAAGAACATTGCGAGCACAAAAGAACGTCATGAAATGCCCAAAGTGTCCACATGACATAGCTGACTTTCTTCAAAATCCACAGGTTAATAAGAATCTTGAGAACGCCATAGAATTACTCCATCAGAAAGAGGAAGATGTTGCAGAGGAAGTGGAAGACGCTGAGGAAGATGAAGATGATGAAGAAGAGGAAGAAGCTGAAGAAACTTACAATGCCGGTGAAGAGAGTGAAGCCGAAGACAAAGGAAGCATCCCACCAATACCTCAGATTGAAACTACAGAGCAACAAACCTGTGAGCAAAAGAAACCAAGTCATAAGCGAAATAGGTCGAGCAATGGAGACAACGTGTTGGATGGCGGAGCACTGACCAAGAGAAGCAAGAGGAGTAAAGTGGGAGCAGCTAGTAGTCTGATTGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

310

Amino Acids

35.22

Weight (kDa)

5.72

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_UBOX PF13445 129 - 161 8.2e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 130
AciI CCGC 3 cut(s) 60, 510, 867
AcuI CTGAAG 1 cut(s) 729
AdeI CACNNNGTG 1 cut(s) 431
AflIII ACRYGT 1 cut(s) 855
AgsI TTSAA 4 cut(s) 49, 187, 584, 782
AjiI CACGTC 1 cut(s) 501
AluBI AGCT 4 cut(s) 452, 572, 707, 911
AluI AGCT 4 cut(s) 452, 572, 707, 911
Alw21I GWGCWC 2 cut(s) 529, 874
Alw26I GTCTC 1 cut(s) 843
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 908
Asp700I GAANNNNTTC 1 cut(s) 366
AspLEI GCGC 2 cut(s) 6, 127
AspS9I GGNCC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 740
AvaII GGWCC 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 672, 753
Bbv12I GWGCWC 2 cut(s) 529, 874
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 920
BccI CCATC 5 cut(s) 25, 61, 308, 639, 857
BceAI ACGGC 1 cut(s) 356
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 843
BfaI CTAG 1 cut(s) 912
BfmI CTRYAG 1 cut(s) 786
BfuAI ACCTGC 1 cut(s) 130
BisI GCNGC 1 cut(s) 909
BlsI GCNGC 1 cut(s) 910
Bme18I GGWCC 1 cut(s) 151
BmgBI CACGTC 1 cut(s) 501
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 1 cut(s) 152
BmsI GCATC 2 cut(s) 229, 768
BpiI GAAGAC 2 cut(s) 672, 753
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 629
BsaJI CCNNGG 3 cut(s) 128, 158, 505
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bse118I RCCGGY 1 cut(s) 725
Bse1I ACTGG 2 cut(s) 297, 416
Bse3DI GCAATG 2 cut(s) 518, 850
BseDI CCNNGG 3 cut(s) 128, 158, 505
BseGI GGATG 3 cut(s) 264, 759, 868
BseMI GCAATG 2 cut(s) 518, 850
BseMII CTCAG 2 cut(s) 663, 788
BseNI ACTGG 2 cut(s) 297, 416
BseRI GAGGAG 1 cut(s) 907
BseXI GCAGC 1 cut(s) 920
BshFI GGCC 1 cut(s) 163
BsiHKAI GWGCWC 2 cut(s) 529, 874
BsiSI CCGG 1 cut(s) 726
BslFI GGGAC 3 cut(s) 32, 164, 245
BsmAI GTCTC 1 cut(s) 843
BsmFI GGGAC 3 cut(s) 32, 164, 245
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 529, 874
Bsp143I GATC 1 cut(s) 142
BspACI CCGC 3 cut(s) 60, 510, 867
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 2 cut(s) 664, 787
BspHI TCATGA 2 cut(s) 540, 929
BspLI GGNNCC 1 cut(s) 152
BspMI ACCTGC 1 cut(s) 130
BsrDI GCAATG 2 cut(s) 518, 850
BsrFI RCCGGY 1 cut(s) 725
BsrI ACTGG 2 cut(s) 297, 416
BssAI RCCGGY 1 cut(s) 725
BssECI CCNNGG 3 cut(s) 128, 158, 505
BssMI GATC 1 cut(s) 142
BssT1I CCWWGG 3 cut(s) 128, 158, 505
Bst4CI ACNGT 2 cut(s) 155, 191
Bst6I CTCTTC 2 cut(s) 690, 726
BstC8I GCNNGC 2 cut(s) 253, 525
BstDEI CTNAG 3 cut(s) 281, 672, 774
BstF5I GGATG 3 cut(s) 264, 759, 868
BstHHI GCGC 2 cut(s) 6, 127
BstKTI GATC 1 cut(s) 145
BstMAI GTCTC 1 cut(s) 843
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstSFI CTRYAG 1 cut(s) 786
BstV1I GCAGC 1 cut(s) 920
BstV2I GAAGAC 2 cut(s) 672, 753
BsuRI GGCC 1 cut(s) 163
BtrI CACGTC 1 cut(s) 501
BtsCI GGATG 3 cut(s) 264, 759, 868
BtsIMutI CAGTG 2 cut(s) 196, 872
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 2 cut(s) 253, 525
CciI TCATGA 2 cut(s) 540, 929
CfoI GCGC 2 cut(s) 6, 127
Cfr10I RCCGGY 1 cut(s) 725
Cfr13I GGNCC 1 cut(s) 151
CseI GACGC 1 cut(s) 677
CviAII CATG 5 cut(s) 146, 429, 541, 563, 930
CviJI RGCY 7 cut(s) 163, 271, 452, 572, 707, 743, 911
CviKI_1 RGCY 7 cut(s) 163, 271, 452, 572, 707, 743, 911
DdeI CTNAG 3 cut(s) 281, 672, 774
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
DraIII CACNNNGTG 1 cut(s) 431
Eam1104I CTCTTC 2 cut(s) 690, 726
EarI CTCTTC 2 cut(s) 690, 726
EciI GGCGGA 3 cut(s) 75, 525, 882
Eco130I CCWWGG 3 cut(s) 128, 158, 505
Eco147I AGGCCT 1 cut(s) 163
Eco32I GATATC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 729
EcoRV GATATC 1 cut(s) 95
EcoT14I CCWWGG 3 cut(s) 128, 158, 505
ErhI CCWWGG 3 cut(s) 128, 158, 505
FaeI CATG 5 cut(s) 149, 432, 544, 566, 933
FaqI GGGAC 3 cut(s) 32, 164, 245
FatI CATG 5 cut(s) 145, 428, 540, 562, 929
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 909
FokI GGATG 3 cut(s) 271, 746, 875
Fsp4HI GCNGC 1 cut(s) 909
FspBI CTAG 1 cut(s) 912
GlaI GCGC 2 cut(s) 5, 126
GluI GCNGC 1 cut(s) 909
HaeIII GGCC 1 cut(s) 163
HapII CCGG 1 cut(s) 726
HgaI GACGC 1 cut(s) 677
HhaI GCGC 2 cut(s) 6, 127
Hin1II CATG 5 cut(s) 149, 432, 544, 566, 933
Hin6I GCGC 2 cut(s) 4, 125
HinP1I GCGC 2 cut(s) 4, 125
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 2 cut(s) 604, 926
HpaII CCGG 1 cut(s) 726
HphI GGTGA 1 cut(s) 740
Hpy166II GTNNAC 2 cut(s) 39, 560
Hpy188I TCNGA 4 cut(s) 399, 636, 777, 921
Hpy188III TCNNGA 3 cut(s) 541, 608, 930
Hpy8I GTNNAC 2 cut(s) 39, 560
HpyAV CCTTC 1 cut(s) 234
HpyCH4III ACNGT 2 cut(s) 155, 191
HpyCH4IV ACGT 3 cut(s) 500, 537, 855
HpyCH4V TGCA 6 cut(s) 90, 179, 242, 447, 460, 653
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 281, 672, 774
HpySE526I ACGT 3 cut(s) 500, 537, 855
Hsp92II CATG 5 cut(s) 149, 432, 544, 566, 933
HspAI GCGC 2 cut(s) 4, 125
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 1 cut(s) 142
LmnI GCTCC 2 cut(s) 869, 905
Lsp1109I GCAGC 1 cut(s) 920
LweI GCATC 2 cut(s) 229, 768
MaeI CTAG 1 cut(s) 912
MaeII ACGT 3 cut(s) 500, 537, 855
MaeIII GTNAC 2 cut(s) 110, 418
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MhlI GDGCHC 2 cut(s) 529, 874
MluCI AATT 2 cut(s) 324, 623
MmeI TCCRAC 1 cut(s) 840
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 3 cut(s) 81, 485, 597
MspI CCGG 1 cut(s) 726
Mva1269I GAATGC 1 cut(s) 358
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeII GATC 1 cut(s) 142
NlaIII CATG 5 cut(s) 149, 432, 544, 566, 933
NlaIV GGNNCC 1 cut(s) 152
NmuCI GTSAC 1 cut(s) 110
PagI TCATGA 2 cut(s) 540, 929
PceI AGGCCT 1 cut(s) 163
PctI GAATGC 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 2 cut(s) 604, 926
PkrI GCNGC 1 cut(s) 910
PspN4I GGNNCC 1 cut(s) 152
PspPI GGNCC 1 cut(s) 151
SaqAI TTAA 3 cut(s) 81, 485, 597
SatI GCNGC 1 cut(s) 909
Sau3AI GATC 1 cut(s) 142
Sau96I GGNCC 1 cut(s) 151
SduI GDGCHC 2 cut(s) 529, 874
SfaNI GCATC 2 cut(s) 229, 768
SfcI CTRYAG 1 cut(s) 786
SinI GGWCC 1 cut(s) 151
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
Sse9I AATT 2 cut(s) 324, 623
SseBI AGGCCT 1 cut(s) 163
SsiI CCGC 3 cut(s) 60, 510, 867
SspMI CTAG 1 cut(s) 912
StuI AGGCCT 1 cut(s) 163
StyI CCWWGG 3 cut(s) 128, 158, 505
TaaI ACNGT 2 cut(s) 155, 191
TaiI ACGT 3 cut(s) 503, 540, 858
TaqI TCGA 2 cut(s) 274, 839
TasI AATT 2 cut(s) 324, 623
TfiI GAWTC 2 cut(s) 604, 926
Tru1I TTAA 3 cut(s) 81, 485, 597
Tru9I TTAA 3 cut(s) 81, 485, 597
TscAI CASTG 2 cut(s) 196, 879
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 908
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 4 cut(s) 557, 696, 705, 918
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 2 cut(s) 196, 879
VpaK11BI GGWCC 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 1 cut(s) 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.