Prupe.1G214700_v2.0.a1

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000644
Physical Location & Seq
Forward (+)
22770634 .. 22774954
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G214700.1

Sequence Viewer

Length: 2220 bp
ATGGCCAACGTCAGCGTTAGAGTGCTCCCCTGCGACGGCGACGGCTTTTGCATGGCCTGCAAAACGAAGCCGTCAGCCGAGGAGACGCTCACGTGCAAAACCTGCTACACCCCCTGGCATGTGGCTTGCCTCGCCGTCCCCCCCGAAACCCTTGCCGAGACTCTCATGTGGGACTGTCCCGACTGTGCTCCGCCGTCCGACGACGGACAGATCGTCGGTAATACGGTCGCCGACGGAGGAGAGGTCGGAAGCTCCGGCGAGCTGATCGCTGCGATCAGAGCCATCGAGTCGGACGAGTCTCTGACCGAGCGGGAGAAGGCAAAGAGGCGGCAGGAGCTGATGTCTGGAGGCGCGCGACCACCGTCCGATGGCGTCGGCGCGAATCCAGAGAAGGACGACAAAACCGTCGGTGAAAACGATGGCCTCGATAATCTCGAGGTCGATATCAAGTGCTCGATTTGCATGCAGCTTCCGGAAAGACCGGTTACGACACCTTGTGGTCACAACTTCTGCCTCAAGTGCTTCCAGAAATGGGTGAAGCAGGGAAAGCGAACCTGCGCGAATTGCCGCTGCGCAGTCCCGCCGAAGATGGCGAGCAACCCTCGCATCAACTCTGTGCTTGTGGAGGCCATCCGCATAGCGAAGCAGTCAAACGGCTTCATCGGAGAGGAACAACGAGCTAAGGTGGTTCACTATGTCCAAATCCAAAACCAGAACCGGCCTGACAAGGCGTTCACCACAGAGAGGGCCAAGAAGGCAGGCAAGGCCAACGCCGCGAGCGGCAGAATCTTCGTGACCATCCCGCCTGACCATTTTGGACCCATTCCGGCCGAAAACAACCCTGAGAAGAAGACGGGGGTGTTGGTTGGGGAGACTTATGACGGCAGGATAGAATGTAGGCAATGGGGGGTTCATTTTCCGATGATTTCTGGGATTGCCGGGCAGTCCAAACATGGGGCCCAGTCGGTTGTTCTCTCTGGTGGCTATGAAGATGATGAGGACCATGGCGAGTGGTTTCTTTACACGGGGAGTGGTGGAAGGGACCTTAGTGGCAATAAGCGTACAAATAAGGCTCACACTTTTGATCAGAAGTTTGAGCTGAGGAATCAGGCACTAAGAGTAAGTTGCCTACAGGGTTACCCTGTTCGAGTTGTCAGATCCAAGAAGGAGAAACGCTCCTCTTATGCCCCGCCAGCAGGGCTACGATACGATGGAGTTTACAGAATTGAGAAATGTTGGACAAAACATGGCAAACAAGGTTTTAAGGTATGCAGGTATCTTTTTGTTAGATGTGACAATGAACCTGCTCCTTGGACAAGTGATGTTCATGGAGACCGTCCAAGGCTTCTGCCAGTTATTCCGGAGTTGGTGAATGCGATTGATGTAACAGAAAGAAGGGTAGAACCTTCTTGGGACTTTGATGAAGAAAAAGGTTGCTGGATGTGGAAAAGGTCTCCACCACGTAGCAAAATACCGGTGGATAGTGGGAACCTAGGAGATAAGAAACAAACTAGGAAAGTTATCAAGCGAAAACAGAAAACGACTGTTAGCAAACGGCTTCTGAAAGGGTTGGAATGCTACCTCTGTCATAATGTTATGACTCTCCCAATTACTACGCCATGTGCTCACAACTTTTGCAAAGATTGCTTAGAGGGCGCCTTTGCTGGCATAAGCGCAACGACACAGCGGACATGCCAAGGCAGACGTACATTACGGGCAAAGAAAAACGTGATGAAGTGTCCTTCATGTACGAATGACATAGCAGATTTTCTTAACAATCCGCAGGTTAATACAGAACTCAAGAATATCATAGAATCACTCCAGGTGAAAGCTGTTAAGTCAGAGGAAGGTGCTAAAGAGTCAAGTAAACATACTGATGGCTCACATGAAAAGCCTGATTTGGTTGCTGAAGACCCCGAAACTCGCAATGCAAGTGAAGCGACTCTGGACGAAGCCAAAGAAGGTGCTGACATTTGTATGAGAGACGCAGAGAGACATGAGGGCAAGGAGCTCTTTAAGCAGCAAGATTCTGTTAACTGTGAGGTAGAACTTGGTCCGGTTGAACCTGCAAAGGCCAACAAGAAGGAACTGCAAGCAACTGCAGTTGAGAGAAACTTAAAGCAGACTCGCAAGCGAAAAAAGGCAAACAACAGAGGTAATGCGTTGGATGTCGGGGCAATGACTAGTAGCAAGAGGGGTAAAGTGGAAGCAGCTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

740

Amino Acids

81.67

Weight (kDa)

8.69

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
Acc16I TGCGCA 1 cut(s) 574
Acc36I ACCTGC 6 cut(s) 110, 563, 1263, 1312, 1774, 2074
AccB1I GGYRCC 1 cut(s) 1655
AccBSI CCGCTC 2 cut(s) 310, 780
AccII CGCG 5 cut(s) 353, 355, 380, 560, 776
AccIII TCCGGA 2 cut(s) 472, 1360
AclWI GGATC 1 cut(s) 1152
AcoI YGGCCR 2 cut(s) 3, 828
AcuI CTGAAG 1 cut(s) 1929
AcvI CACGTG 1 cut(s) 93
AcyI GRCGYC 2 cut(s) 372, 1656
AdeI CACNNNGTG 1 cut(s) 497
AfaI GTAC 3 cut(s) 1063, 1708, 1750
AfiI CCNNNNNNNGG 6 cut(s) 35, 368, 532, 744, 954, 1196
AgeI ACCGGT 2 cut(s) 481, 1474
AgsI TTSAA 1 cut(s) 2063
AhdI GACNNNNNGTC 1 cut(s) 212
AhlI ACTAGT 1 cut(s) 2183
AjnI CCWGG 2 cut(s) 113, 1821
AluBI AGCT 9 cut(s) 252, 262, 337, 469, 680, 1099, 1832, 2011, 2213
AluI AGCT 9 cut(s) 252, 262, 337, 469, 680, 1099, 1832, 2011, 2213
Alw21I GWGCWC 5 cut(s) 27, 190, 455, 1627, 2013
Alw26I GTCTC 8 cut(s) 77, 152, 303, 866, 1326, 1458, 1977, 1987
AlwI GGATC 1 cut(s) 1152
AlwNI CAGNNNCTG 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 434
Aor13HI TCCGGA 2 cut(s) 472, 1360
ApaI GGGCCC 1 cut(s) 961
ApeKI GCWGC 6 cut(s) 269, 466, 570, 2020, 2210, 2213
AsiGI ACCGGT 2 cut(s) 481, 1474
AspA2I CCTAGG 1 cut(s) 1492
AspLEI GCGC 7 cut(s) 353, 355, 380, 560, 575, 1658, 1676
AspS9I GGNCC 7 cut(s) 747, 818, 957, 958, 1000, 1042, 2054
AsuC2I CCSGG 1 cut(s) 940
AsuHPI GGTGA 5 cut(s) 422, 547, 727, 1381, 1837
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 4 cut(s) 818, 1000, 1042, 2054
AvrII CCTAGG 1 cut(s) 1492
BaeGI GKGCMC 1 cut(s) 961
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 1655
BanII GRGCYC 2 cut(s) 961, 2013
BbrPI CACGTG 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 857, 1917
Bbv12I GWGCWC 5 cut(s) 27, 190, 455, 1627, 2013
BbvCI CCTCAGC 1 cut(s) 1100
BbvI GCAGC 5 cut(s) 256, 478, 557, 2032, 2200
BccI CCATC 8 cut(s) 290, 362, 413, 583, 638, 806, 1205, 1871
BceAI ACGGC 8 cut(s) 52, 55, 58, 119, 178, 670, 898, 1571
BcgI CGANNNNNNTGC 6 cut(s) 134, 168, 445, 479, 772, 806
BciT130I CCWGG 2 cut(s) 115, 1823
BclI TGATCA 1 cut(s) 1084
BcnI CCSGG 1 cut(s) 940
BcoDI GTCTC 8 cut(s) 77, 152, 303, 866, 1326, 1458, 1977, 1987
BcuI ACTAGT 1 cut(s) 2183
BfaI CTAG 3 cut(s) 1493, 1512, 2184
BfmI CTRYAG 2 cut(s) 1130, 2100
BfoI RGCGCY 1 cut(s) 1659
BfuAI ACCTGC 6 cut(s) 110, 563, 1263, 1312, 1774, 2074
BglI GCCNNNNNGGC 2 cut(s) 755, 1198
BlnI CCTAGG 1 cut(s) 1492
Bme1390I CCNGG 3 cut(s) 115, 940, 1823
Bme18I GGWCC 4 cut(s) 818, 1000, 1042, 2054
BmeRI GACNNNNNGTC 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 7 cut(s) 747, 818, 957, 958, 1000, 1042, 2054
BmiI GGNNCC 6 cut(s) 820, 958, 959, 1043, 1490, 1657
BmrFI CCNGG 3 cut(s) 115, 940, 1823
BmrI ACTGGG 1 cut(s) 955
BmsI GCATC 1 cut(s) 615
BmuI ACTGGG 1 cut(s) 955
BoxI GACNNNNGTC 1 cut(s) 361
BpiI GAAGAC 2 cut(s) 857, 1917
BplI GAGNNNNNCTC 4 cut(s) 586, 618, 1160, 1192
BpmI CTGGAG 2 cut(s) 366, 1805
Bpu10I CCTNAGC 2 cut(s) 681, 1100
BpuEI CTTGAG 2 cut(s) 500, 1784
BpuMI CCSGG 1 cut(s) 940
BsaAI YACGTR 2 cut(s) 93, 1463
BsaHI GRCGYC 2 cut(s) 372, 1656
BsaI GGTCTC 2 cut(s) 1326, 1458
BsaJI CCNNGG 7 cut(s) 78, 113, 1003, 1310, 1340, 1492, 1696
BsaWI WCCGGW 5 cut(s) 472, 481, 1360, 1474, 2056
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 326, 356
Bsc4I CCNNNNNNNGG 6 cut(s) 35, 368, 532, 744, 954, 1196
Bse118I RCCGGY 3 cut(s) 481, 717, 1474
Bse1I ACTGG 2 cut(s) 961, 1352
Bse3DI GCAATG 3 cut(s) 908, 1933, 2184
BseAI TCCGGA 2 cut(s) 472, 1360
BseBI CCWGG 2 cut(s) 115, 1823
BseDI CCNNGG 7 cut(s) 78, 113, 1003, 1310, 1340, 1492, 1696
BseGI GGATG 4 cut(s) 630, 798, 1446, 2173
BseLI CCNNNNNNNGG 6 cut(s) 35, 368, 532, 744, 954, 1196
BseMI GCAATG 3 cut(s) 908, 1933, 2184
BseMII CTCAG 2 cut(s) 834, 1091
BseNI ACTGG 2 cut(s) 961, 1352
BsePI GCGCGC 1 cut(s) 351
BseRI GAGGAG 3 cut(s) 95, 252, 1168
BseSI GKGCMC 1 cut(s) 961
BseX3I CGGCCG 1 cut(s) 828
BseXI GCAGC 5 cut(s) 256, 478, 557, 2032, 2200
Bsh1236I CGCG 5 cut(s) 353, 355, 380, 560, 776
Bsh1285I CGRYCG 2 cut(s) 228, 831
BshNI GGYRCC 1 cut(s) 1655
BshTI ACCGGT 2 cut(s) 481, 1474
BsiEI CGRYCG 2 cut(s) 228, 831
BsiHKAI GWGCWC 5 cut(s) 27, 190, 455, 1627, 2013
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 9 cut(s) 255, 473, 482, 718, 827, 939, 1361, 1475, 2057
BslFI GGGAC 6 cut(s) 122, 162, 185, 563, 1055, 1427
BslI CCNNNNNNNGG 6 cut(s) 35, 368, 532, 744, 954, 1196
BsmAI GTCTC 8 cut(s) 77, 152, 303, 866, 1326, 1458, 1977, 1987
BsmBI CGTCTC 2 cut(s) 77, 1977
BsmFI GGGAC 6 cut(s) 122, 162, 185, 563, 1055, 1427
BsmI GAATGC 2 cut(s) 1378, 1580
Bso31I GGTCTC 2 cut(s) 1326, 1458
BsoBI CYCGRG 1 cut(s) 434
Bsp120I GGGCCC 1 cut(s) 957
Bsp1286I GDGCHC 6 cut(s) 27, 190, 455, 961, 1627, 2013
Bsp13I TCCGGA 2 cut(s) 472, 1360
Bsp143I GATC 5 cut(s) 210, 264, 273, 1084, 1157
Bsp19I CCATGG 1 cut(s) 1003
BspCNI CTCAG 2 cut(s) 835, 1092
BspEI TCCGGA 2 cut(s) 472, 1360
BspFNI CGCG 5 cut(s) 353, 355, 380, 560, 776
BspLI GGNNCC 6 cut(s) 820, 958, 959, 1043, 1490, 1657
BspMAI CTGCAG 1 cut(s) 2104
BspMI ACCTGC 6 cut(s) 110, 563, 1263, 1312, 1774, 2074
BspPI GGATC 1 cut(s) 1152
BspT107I GGYRCC 1 cut(s) 1655
BspTNI GGTCTC 2 cut(s) 1326, 1458
BsrBI CCGCTC 2 cut(s) 310, 780
BsrDI GCAATG 3 cut(s) 908, 1933, 2184
BsrFI RCCGGY 3 cut(s) 481, 717, 1474
BsrI ACTGG 2 cut(s) 961, 1352
BssAI RCCGGY 3 cut(s) 481, 717, 1474
BssECI CCNNGG 7 cut(s) 78, 113, 1003, 1310, 1340, 1492, 1696
BssHII GCGCGC 1 cut(s) 351
BssMI GATC 5 cut(s) 210, 264, 273, 1084, 1157
BssNI GRCGYC 2 cut(s) 372, 1656
BssT1I CCWWGG 5 cut(s) 1003, 1310, 1340, 1492, 1696
Bst2UI CCWGG 2 cut(s) 115, 1823
Bst4CI ACNGT 8 cut(s) 176, 185, 226, 363, 406, 1337, 1546, 2039
BstACI GRCGYC 2 cut(s) 372, 1656
BstAPI GCANNNNNTGC 3 cut(s) 57, 102, 1644
BstBAI YACGTR 2 cut(s) 93, 1463
BstDEI CTNAG 6 cut(s) 681, 843, 1046, 1100, 1115, 1648
BstDSI CCRYGG 1 cut(s) 1003
BstEII GGTNACC 1 cut(s) 1136
BstF5I GGATG 4 cut(s) 630, 798, 1446, 2173
BstFNI CGCG 5 cut(s) 353, 355, 380, 560, 776
BstH2I RGCGCY 1 cut(s) 1659
BstHHI GCGC 7 cut(s) 353, 355, 380, 560, 575, 1658, 1676
BstKTI GATC 5 cut(s) 213, 267, 276, 1087, 1160
BstMAI GTCTC 8 cut(s) 77, 152, 303, 866, 1326, 1458, 1977, 1987
BstMBI GATC 5 cut(s) 210, 264, 273, 1084, 1157
BstMCI CGRYCG 2 cut(s) 228, 831
BstNI CCWGG 2 cut(s) 115, 1823
BstNSI RCATGY 3 cut(s) 122, 466, 1695
BstPAI GACNNNNGTC 1 cut(s) 361
BstPI GGTNACC 1 cut(s) 1136
BstSCI CCNGG 3 cut(s) 113, 938, 1821
BstSFI CTRYAG 2 cut(s) 1130, 2100
BstSLI GKGCMC 1 cut(s) 961
BstUI CGCG 5 cut(s) 353, 355, 380, 560, 776
BstV1I GCAGC 5 cut(s) 256, 478, 557, 2032, 2200
BstV2I GAAGAC 2 cut(s) 857, 1917
BstX2I RGATCY 1 cut(s) 1157
BstYI RGATCY 1 cut(s) 1157
BstZI CGGCCG 1 cut(s) 828
BtgI CCRYGG 1 cut(s) 1003
BtsCI GGATG 4 cut(s) 630, 798, 1446, 2173
BveI ACCTGC 6 cut(s) 110, 563, 1263, 1312, 1774, 2074
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 7 cut(s) 353, 355, 380, 560, 575, 1658, 1676
Cfr10I RCCGGY 3 cut(s) 481, 717, 1474
Cfr13I GGNCC 7 cut(s) 747, 818, 957, 958, 1000, 1042, 2054
CseI GACGC 3 cut(s) 94, 361, 1994
Csp6I GTAC 3 cut(s) 1062, 1707, 1749
CspAI ACCGGT 2 cut(s) 481, 1474
CviQI GTAC 3 cut(s) 1062, 1707, 1749
DdeI CTNAG 6 cut(s) 681, 843, 1046, 1100, 1115, 1648
DinI GGCGCC 1 cut(s) 1657
DpnI GATC 5 cut(s) 212, 266, 275, 1086, 1159
DpnII GATC 5 cut(s) 210, 264, 273, 1084, 1157
DraIII CACNNNGTG 1 cut(s) 497
DrdI GACNNNNNNGTC 1 cut(s) 404
DriI GACNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 404
EaeI YGGCCR 2 cut(s) 3, 828
EagI CGGCCG 1 cut(s) 828
Eam1105I GACNNNNNGTC 1 cut(s) 212
EciI GGCGGA 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 2011
EclXI CGGCCG 1 cut(s) 828
Eco130I CCWWGG 5 cut(s) 1003, 1310, 1340, 1492, 1696
Eco24I GRGCYC 2 cut(s) 961, 2013
Eco31I GGTCTC 2 cut(s) 1326, 1458
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 4 cut(s) 818, 1000, 1042, 2054
Eco52I CGGCCG 1 cut(s) 828
Eco53kI GAGCTC 1 cut(s) 2011
Eco57I CTGAAG 1 cut(s) 1929
Eco72I CACGTG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 434
Eco91I GGTNACC 1 cut(s) 1136
EcoICRI GAGCTC 1 cut(s) 2011
EcoO109I RGGNCCY 2 cut(s) 957, 1042
EcoO65I GGTNACC 1 cut(s) 1136
EcoRII CCWGG 2 cut(s) 113, 1821
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 5 cut(s) 1003, 1310, 1340, 1492, 1696
EcoT38I GRGCYC 2 cut(s) 961, 2013
EgeI GGCGCC 1 cut(s) 1657
EheI GGCGCC 1 cut(s) 1657
ErhI CCWWGG 5 cut(s) 1003, 1310, 1340, 1492, 1696
Esp3I CGTCTC 2 cut(s) 77, 1977
FalI AAGNNNNNCTT 2 cut(s) 1997, 2029
FaqI GGGAC 6 cut(s) 122, 162, 185, 563, 1055, 1427
FauI CCCGC 4 cut(s) 303, 588, 810, 1197
FbaI TGATCA 1 cut(s) 1084
FokI GGATG 4 cut(s) 617, 785, 1453, 2180
FriOI GRGCYC 2 cut(s) 961, 2013
FspBI CTAG 3 cut(s) 1493, 1512, 2184
FspI TGCGCA 1 cut(s) 574
GlaI GCGC 7 cut(s) 352, 354, 379, 559, 574, 1657, 1675
GsuI CTGGAG 2 cut(s) 366, 1805
HaeII RGCGCY 1 cut(s) 1659
HapII CCGG 9 cut(s) 255, 473, 482, 718, 827, 939, 1361, 1475, 2057
HgaI GACGC 3 cut(s) 94, 361, 1994
HhaI GCGC 7 cut(s) 353, 355, 380, 560, 575, 1658, 1676
Hin1I GRCGYC 2 cut(s) 372, 1656
Hin6I GCGC 7 cut(s) 351, 353, 378, 558, 573, 1656, 1674
HinP1I GCGC 7 cut(s) 351, 353, 378, 558, 573, 1656, 1674
HincII GTYRAC 1 cut(s) 2035
HindII GTYRAC 1 cut(s) 2035
HpaI GTTAAC 1 cut(s) 2035
HpaII CCGG 9 cut(s) 255, 473, 482, 718, 827, 939, 1361, 1475, 2057
HphI GGTGA 5 cut(s) 422, 547, 727, 1381, 1837
Hpy166II GTNNAC 5 cut(s) 691, 735, 1219, 1868, 2035
Hpy8I GTNNAC 5 cut(s) 691, 735, 1219, 1868, 2035
Hpy99I CGWCG 8 cut(s) 38, 44, 203, 206, 218, 236, 377, 410
HpyCH4III ACNGT 8 cut(s) 176, 185, 226, 363, 406, 1337, 1546, 2039
HpyCH4IV ACGT 5 cut(s) 9, 92, 1462, 1705, 1728
HpyF3I CTNAG 6 cut(s) 681, 843, 1046, 1100, 1115, 1648
HpySE526I ACGT 5 cut(s) 9, 92, 1462, 1705, 1728
Hsp92I GRCGYC 2 cut(s) 372, 1656
HspAI GCGC 7 cut(s) 351, 353, 378, 558, 573, 1656, 1674
KasI GGCGCC 1 cut(s) 1655
Kpn2I TCCGGA 2 cut(s) 472, 1360
Ksp22I TGATCA 1 cut(s) 1084
KspAI GTTAAC 1 cut(s) 2035
Kzo9I GATC 5 cut(s) 210, 264, 273, 1084, 1157
LmnI GCTCC 7 cut(s) 30, 193, 257, 334, 1181, 1312, 2008
Lsp1109I GCAGC 5 cut(s) 256, 478, 557, 2032, 2200
LweI GCATC 1 cut(s) 615
MaeI CTAG 3 cut(s) 1493, 1512, 2184
MaeII ACGT 5 cut(s) 9, 92, 1462, 1705, 1728
MaeIII GTNAC 6 cut(s) 484, 500, 793, 1136, 1292, 1384
MalI GATC 5 cut(s) 212, 266, 275, 1086, 1159
MbiI CCGCTC 2 cut(s) 310, 780
MboI GATC 5 cut(s) 210, 264, 273, 1084, 1157
MboII GAAGA 7 cut(s) 598, 781, 859, 862, 1001, 1436, 1922
MflI RGATCY 1 cut(s) 1157
MhlI GDGCHC 6 cut(s) 27, 190, 455, 961, 1627, 2013
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 562, 1224, 1608
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 1656
MlyI GAGTC 7 cut(s) 154, 296, 305, 1594, 1868, 1936, 2119
MmeI TCCRAC 6 cut(s) 222, 226, 270, 1217, 1551, 2145
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 472, 1360
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 1263, 1773, 1788, 1836, 2016, 2034, 2117
MslI CAYNNNNRTG 2 cut(s) 1875, 1976
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 570, 1687, 2213
MspI CCGG 9 cut(s) 255, 473, 482, 718, 827, 939, 1361, 1475, 2057
MspR9I CCNGG 3 cut(s) 115, 940, 1823
Mva1269I GAATGC 2 cut(s) 1378, 1580
MvaI CCWGG 2 cut(s) 115, 1823
MvnI CGCG 5 cut(s) 353, 355, 380, 560, 776
NarI GGCGCC 1 cut(s) 1656
NciI CCSGG 1 cut(s) 940
NcoI CCATGG 1 cut(s) 1003
NdeII GATC 5 cut(s) 210, 264, 273, 1084, 1157
NlaIV GGNNCC 6 cut(s) 820, 958, 959, 1043, 1490, 1657
NmeAIII GCCGAG 2 cut(s) 103, 181
NmuCI GTSAC 3 cut(s) 500, 793, 1292
NsbI TGCGCA 1 cut(s) 574
NspI RCATGY 3 cut(s) 122, 466, 1695
PaeI GCATGC 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 434
PauI GCGCGC 1 cut(s) 351
PcsI WCGNNNNNNNCGW 6 cut(s) 210, 291, 402, 414, 423, 432
PctI GAATGC 2 cut(s) 1378, 1580
PfeI GAWTC 5 cut(s) 382, 786, 1105, 1814, 2027
PinAI ACCGGT 2 cut(s) 481, 1474
PleI GAGTC 7 cut(s) 154, 295, 304, 1594, 1867, 1936, 2119
PluTI GGCGCC 1 cut(s) 1659
PmaCI CACGTG 1 cut(s) 93
PmlI CACGTG 1 cut(s) 93
PpsI GAGTC 7 cut(s) 154, 295, 304, 1594, 1867, 1936, 2119
Ppu21I YACGTR 2 cut(s) 93, 1463
PpuMI RGGWCCY 1 cut(s) 1042
PshAI GACNNNNGTC 1 cut(s) 361
Psp124BI GAGCTC 1 cut(s) 2013
Psp5II RGGWCCY 1 cut(s) 1042
Psp6I CCWGG 2 cut(s) 113, 1821
PspCI CACGTG 1 cut(s) 93
PspEI GGTNACC 1 cut(s) 1136
PspGI CCWGG 2 cut(s) 113, 1821
PspN4I GGNNCC 6 cut(s) 820, 958, 959, 1043, 1490, 1657
PspOMI GGGCCC 1 cut(s) 957
PspPI GGNCC 7 cut(s) 747, 818, 957, 958, 1000, 1042, 2054
PspPPI RGGWCCY 1 cut(s) 1042
PstI CTGCAG 1 cut(s) 2104
PstNI CAGNNNCTG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 1157
PteI GCGCGC 1 cut(s) 351
PvuII CAGCTG 1 cut(s) 2213
RsaI GTAC 3 cut(s) 1063, 1708, 1750
RsaNI GTAC 3 cut(s) 1062, 1707, 1749
RseI CAYNNNNRTG 2 cut(s) 1875, 1976
SacI GAGCTC 1 cut(s) 2013
SaqAI TTAA 7 cut(s) 1263, 1773, 1788, 1836, 2016, 2034, 2117
Sau3AI GATC 5 cut(s) 210, 264, 273, 1084, 1157
Sau96I GGNCC 7 cut(s) 747, 818, 957, 958, 1000, 1042, 2054
SchI GAGTC 7 cut(s) 154, 296, 305, 1594, 1868, 1936, 2119
ScrFI CCNGG 3 cut(s) 115, 940, 1823
SduI GDGCHC 6 cut(s) 27, 190, 455, 961, 1627, 2013
SfaNI GCATC 1 cut(s) 615
SfcI CTRYAG 2 cut(s) 1130, 2100
SfoI GGCGCC 1 cut(s) 1657
Sfr274I CTCGAG 1 cut(s) 434
SinI GGWCC 4 cut(s) 818, 1000, 1042, 2054
SlaI CTCGAG 1 cut(s) 434
SmiMI CAYNNNNRTG 2 cut(s) 1875, 1976
SmlI CTYRAG 3 cut(s) 434, 515, 1799
SmoI CTYRAG 3 cut(s) 434, 515, 1799
SpeI ACTAGT 1 cut(s) 2183
SphI GCATGC 1 cut(s) 466
Sse9I AATT 3 cut(s) 562, 1224, 1608
SspDI GGCGCC 1 cut(s) 1655
SspMI CTAG 3 cut(s) 1493, 1512, 2184
SstI GAGCTC 1 cut(s) 2013
StyD4I CCNGG 3 cut(s) 113, 938, 1821
StyI CCWWGG 5 cut(s) 1003, 1310, 1340, 1492, 1696
TaaI ACNGT 8 cut(s) 176, 185, 226, 363, 406, 1337, 1546, 2039
TaiI ACGT 5 cut(s) 12, 95, 1465, 1708, 1731
TaqI TCGA 6 cut(s) 285, 426, 435, 441, 455, 1147
TaqII GACCGA 1 cut(s) 320
TasI AATT 3 cut(s) 562, 1224, 1608
TauI GCSGC 4 cut(s) 331, 570, 776, 783
TfiI GAWTC 5 cut(s) 382, 786, 1105, 1814, 2027
Tru1I TTAA 7 cut(s) 1263, 1773, 1788, 1836, 2016, 2034, 2117
Tru9I TTAA 7 cut(s) 1263, 1773, 1788, 1836, 2016, 2034, 2117
TseFI GTSAC 3 cut(s) 500, 793, 1292
TseI GCWGC 6 cut(s) 269, 466, 570, 2020, 2210, 2213
Tsp45I GTSAC 3 cut(s) 500, 793, 1292
TspDTI ATGAA 9 cut(s) 649, 902, 1002, 1314, 1316, 1437, 1734, 1748, 1902
TspGWI ACGGA 2 cut(s) 219, 249
VpaK11BI GGWCC 4 cut(s) 818, 1000, 1042, 2054
XceI RCATGY 3 cut(s) 122, 466, 1695
XhoI CTCGAG 1 cut(s) 434
XmaJI CCTAGG 1 cut(s) 1492
XspI CTAG 3 cut(s) 1493, 1512, 2184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.