pycom06g01280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013635
Physical Location & Seq
Reverse (-)
1325392 .. 1327849
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g01280.4

Sequence Viewer

Length: 660 bp
ATGAAGAAAAGCGGCGGAAGCGGAAGCGGAAGTCGAACCCGGAGATGCTTAACCGCGAACTTTCTGGAAACGCGGTTACAATTTCCAGTGGCTTCTTCCCCTGCAAACCAACCGTCACCGAAATCACCAATTTTCAATGCCAGCAGCAGGCGCAAGAGGGCTGCCGACGCTGCCGGAGGAGGAGCCTCAGCCTCTGTTGTGGCGGCGCTCATGGAATCCCCCAAAAGTAACGCTTTAGCTTCGATTCCTGATCTCAAGCACCTCGCCTCCTCTCGTCTCCGCGATCTCAAGCTCCACGTCGACAGATCCCACTGTGAGATCCTCAAGGATTTTGATTCCTCTCAATCTCGCCTCCACAAGCGATTCAAGATCCAGAGCCAGGCATGCCAACAAGTGTTGGATGAAGCAGATAAGGAATACAAGAAGATGACCCAAAGGATTACTGAAAGTCGGGAAGCAATGGCGAGTTCATGGCAGATGCACAGACCACCGCTTGTCGTGCTTGCAAAACATCAATCGCTGAGCTTTCGCAGTCCTTTGAGAAACAAATTGATGCTCTTCGCAGCCGTTATGGCATTCAAACAAATTAGCAATCAATTGTCCTTGCAATATTATCTACTGAAAACCTTAGGCATGTATAGTGGTGACGAAAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

24.28

Weight (kDa)

10.2

Isoelectric Point (pI)

71.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08760 FvH4_5g24040
malus_domestica MD06G1015900.v1.1 MD06G1016300.v1.1 MD06G1016600.v1.1
prunus_persica Prupe.5G019800_v2.0.a1
pyrus_communis pycom06g01280
rosa_chinensis RchiOBHm_Chr7g0213521
rosa_laevigata RLG00000002798
rosa_multiflora Rmu_sc0010076.1_g000002
rosa_roxburghii Rroxscaffold_3G00245810
rosa_rugosa Rorug07G0142700
rosa_samantha Rh7AG275900 Rh7BG270300 Rh7CG294800 Rh7DG282500
rosa_wichuraiana Rw0G023480 Rw7G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 300
AccII CGCG 3 cut(s) 56, 73, 282
AciI CCGC 9 cut(s) 12, 15, 21, 27, 54, 73, 203, 280, 491
AclWI GGATC 3 cut(s) 300, 313, 364
AfiI CCNNNNNNNGG 2 cut(s) 147, 379
AgsI TTSAA 3 cut(s) 136, 367, 580
AjiI CACGTC 1 cut(s) 298
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 3 cut(s) 239, 292, 525
AluI AGCT 3 cut(s) 239, 292, 525
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 3 cut(s) 300, 313, 364
AlwNI CAGNNNCTG 1 cut(s) 194
ApeKI GCWGC 4 cut(s) 144, 161, 170, 563
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AspLEI GCGC 2 cut(s) 153, 208
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 3 cut(s) 108, 117, 656
AxyI CCTNAGG 1 cut(s) 628
BbvCI CCTCAGC 1 cut(s) 187
BbvI GCAGC 4 cut(s) 148, 156, 157, 575
BceAI ACGGC 1 cut(s) 551
BciT130I CCWGG 1 cut(s) 380
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 281
BfoI RGCGCY 1 cut(s) 209
BglI GCCNNNNNGGC 1 cut(s) 572
BisI GCNGC 6 cut(s) 13, 145, 162, 171, 204, 564
BlpI GCTNAGC 1 cut(s) 521
BlsI GCNGC 6 cut(s) 14, 146, 163, 172, 205, 565
Bme1390I CCNGG 2 cut(s) 40, 380
BmgBI CACGTC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 2 cut(s) 40, 380
BmsI GCATC 3 cut(s) 35, 468, 543
Bpu10I CCTNAGC 1 cut(s) 187
Bpu1102I GCTNAGC 1 cut(s) 521
BpuEI CTTGAG 3 cut(s) 239, 272, 308
BpuMI CCSGG 1 cut(s) 40
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 379
Bse1I ACTGG 1 cut(s) 86
Bse21I CCTNAGG 1 cut(s) 628
Bse3DI GCAATG 1 cut(s) 465
BseBI CCWGG 1 cut(s) 380
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 2 cut(s) 147, 379
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 2 cut(s) 201, 512
BseNI ACTGG 1 cut(s) 86
BseRI GAGGAG 3 cut(s) 192, 195, 259
BseXI GCAGC 4 cut(s) 148, 156, 157, 575
Bsh1236I CGCG 3 cut(s) 56, 73, 282
BsiSI CCGG 2 cut(s) 40, 174
BslI CCNNNNNNNGG 2 cut(s) 147, 379
BsmAI GTCTC 1 cut(s) 281
BsmBI CGTCTC 1 cut(s) 281
BsmI GAATGC 1 cut(s) 575
Bsp143I GATC 5 cut(s) 250, 283, 305, 318, 369
Bsp1720I GCTNAGC 1 cut(s) 521
BspACI CCGC 9 cut(s) 12, 15, 21, 27, 54, 73, 203, 280, 491
BspCNI CTCAG 2 cut(s) 200, 513
BspFNI CGCG 3 cut(s) 56, 73, 282
BspLI GGNNCC 1 cut(s) 184
BspPI GGATC 3 cut(s) 300, 313, 364
BspQI GCTCTTC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 465
BsrI ACTGG 1 cut(s) 86
BssMI GATC 5 cut(s) 250, 283, 305, 318, 369
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 2 cut(s) 114, 314
Bst6I CTCTTC 1 cut(s) 563
BstC8I GCNNGC 4 cut(s) 142, 149, 385, 504
BstDEI CTNAG 3 cut(s) 187, 521, 628
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 3 cut(s) 56, 73, 282
BstH2I RGCGCY 1 cut(s) 209
BstHHI GCGC 2 cut(s) 153, 208
BstKTI GATC 5 cut(s) 253, 286, 308, 321, 372
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 5 cut(s) 250, 283, 305, 318, 369
BstMWI GCNNNNNNNGC 7 cut(s) 18, 150, 167, 170, 384, 499, 572
BstNI CCWGG 1 cut(s) 380
BstNSI RCATGY 2 cut(s) 387, 637
BstSCI CCNGG 2 cut(s) 38, 378
BstUI CGCG 3 cut(s) 56, 73, 282
BstV1I GCAGC 4 cut(s) 148, 156, 157, 575
BstX2I RGATCY 3 cut(s) 305, 318, 369
BstYI RGATCY 3 cut(s) 305, 318, 369
Bsu36I CCTNAGG 1 cut(s) 628
BtrI CACGTC 1 cut(s) 298
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 2 cut(s) 93, 310
Cac8I GCNNGC 4 cut(s) 142, 149, 385, 504
CaiI CAGNNNCTG 1 cut(s) 194
CfoI GCGC 2 cut(s) 153, 208
CseI GACGC 1 cut(s) 176
CviAII CATG 4 cut(s) 211, 384, 471, 634
CviJI RGCY 9 cut(s) 92, 161, 185, 191, 239, 292, 378, 525, 566
CviKI_1 RGCY 9 cut(s) 92, 161, 185, 191, 239, 292, 378, 525, 566
DdeI CTNAG 3 cut(s) 187, 521, 628
DpnI GATC 5 cut(s) 252, 285, 307, 320, 371
DpnII GATC 5 cut(s) 250, 283, 305, 318, 369
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
EciI GGCGGA 1 cut(s) 30
Eco81I CCTNAGG 1 cut(s) 628
EcoRII CCWGG 1 cut(s) 378
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 4 cut(s) 214, 387, 474, 637
FaiI YATR 6 cut(s) 212, 385, 472, 572, 635, 639
FalI AAGNNNNNCTT 2 cut(s) 217, 249
FatI CATG 4 cut(s) 210, 383, 470, 633
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 6 cut(s) 13, 145, 162, 171, 204, 564
FokI GGATG 1 cut(s) 413
Fsp4HI GCNGC 6 cut(s) 13, 145, 162, 171, 204, 564
GlaI GCGC 2 cut(s) 152, 207
GluI GCNGC 6 cut(s) 13, 145, 162, 171, 204, 564
HaeII RGCGCY 1 cut(s) 209
HapII CCGG 2 cut(s) 40, 174
HgaI GACGC 1 cut(s) 176
HhaI GCGC 2 cut(s) 153, 208
Hin1II CATG 4 cut(s) 214, 387, 474, 637
Hin6I GCGC 2 cut(s) 151, 206
HinP1I GCGC 2 cut(s) 151, 206
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 4 cut(s) 215, 244, 335, 363
HpaII CCGG 2 cut(s) 40, 174
HphI GGTGA 3 cut(s) 108, 117, 656
Hpy166II GTNNAC 1 cut(s) 301
Hpy188III TCNNGA 5 cut(s) 65, 248, 367, 373, 452
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 2 cut(s) 170, 302
HpyCH4III ACNGT 2 cut(s) 114, 314
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 4 cut(s) 104, 481, 506, 607
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 150, 167, 170, 384, 499, 572
HpyF3I CTNAG 3 cut(s) 187, 521, 628
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 4 cut(s) 214, 387, 474, 637
HspAI GCGC 2 cut(s) 151, 206
Kzo9I GATC 5 cut(s) 250, 283, 305, 318, 369
LguI GCTCTTC 1 cut(s) 563
LmnI GCTCC 2 cut(s) 182, 297
Lsp1109I GCAGC 4 cut(s) 148, 156, 157, 575
LweI GCATC 3 cut(s) 35, 468, 543
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 4 cut(s) 75, 114, 227, 644
MalI GATC 5 cut(s) 252, 285, 307, 320, 371
MboI GATC 5 cut(s) 250, 283, 305, 318, 369
MboII GAAGA 4 cut(s) 16, 87, 436, 550
MfeI CAATTG 2 cut(s) 596, 655
MflI RGATCY 3 cut(s) 305, 318, 369
MluCI AATT 6 cut(s) 80, 129, 548, 585, 596, 655
MmeI TCCRAC 1 cut(s) 378
MseI TTAA 1 cut(s) 50
MspI CCGG 2 cut(s) 40, 174
MspR9I CCNGG 2 cut(s) 40, 380
MunI CAATTG 2 cut(s) 596, 655
Mva1269I GAATGC 1 cut(s) 575
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 3 cut(s) 56, 73, 282
MwoI GCNNNNNNNGC 7 cut(s) 18, 150, 167, 170, 384, 499, 572
NciI CCSGG 1 cut(s) 40
NdeII GATC 5 cut(s) 250, 283, 305, 318, 369
NlaIII CATG 4 cut(s) 214, 387, 474, 637
NlaIV GGNNCC 1 cut(s) 184
NmuCI GTSAC 2 cut(s) 114, 644
NspI RCATGY 2 cut(s) 387, 637
PaeI GCATGC 1 cut(s) 387
PciSI GCTCTTC 1 cut(s) 563
PctI GAATGC 1 cut(s) 575
PfeI GAWTC 4 cut(s) 215, 244, 335, 363
PkrI GCNGC 6 cut(s) 14, 146, 163, 172, 205, 565
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 3 cut(s) 305, 318, 369
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 563
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 6 cut(s) 13, 145, 162, 171, 204, 564
Sau3AI GATC 5 cut(s) 250, 283, 305, 318, 369
ScrFI CCNGG 2 cut(s) 40, 380
SetI ASST 6 cut(s) 241, 264, 294, 300, 527, 629
SfaNI GCATC 3 cut(s) 35, 468, 543
SmlI CTYRAG 3 cut(s) 254, 287, 323
SmoI CTYRAG 3 cut(s) 254, 287, 323
SphI GCATGC 1 cut(s) 387
Sse9I AATT 6 cut(s) 80, 129, 548, 585, 596, 655
SsiI CCGC 9 cut(s) 12, 15, 21, 27, 54, 73, 203, 280, 491
SspI AATATT 1 cut(s) 611
StyD4I CCNGG 2 cut(s) 38, 378
TaaI ACNGT 2 cut(s) 114, 314
TaiI ACGT 1 cut(s) 300
TaqI TCGA 3 cut(s) 34, 242, 300
TasI AATT 6 cut(s) 80, 129, 548, 585, 596, 655
TauI GCSGC 2 cut(s) 15, 206
TfiI GAWTC 4 cut(s) 215, 244, 335, 363
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 2 cut(s) 93, 317
TseFI GTSAC 2 cut(s) 114, 644
TseI GCWGC 4 cut(s) 144, 161, 170, 563
Tsp45I GTSAC 2 cut(s) 114, 644
TspDTI ATGAA 3 cut(s) 17, 417, 459
TspRI CASTG 2 cut(s) 93, 317
XceI RCATGY 2 cut(s) 387, 637
XmiI GTMKAC 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.