MD06G1017500.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011373
Physical Location & Seq
Forward (+)
2143066 .. 2144199
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1017500.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGCAATGTCAGAGGCAAAGACTTCCATATCTCCCTCAGAAAGCAAGAAACGGAGTCAAAACGACGACCAACATCAGCCCCTCCTGCCTGGTCTGCCGGACAGCATAGCCCTACTCTCCCTCTCCCTCGTCCATCCTTCTGTTTTGTTCTCCGTTTGCACCTCATGGCGCCGCCTTATCTACTCCTCCTCCTTCCCTCCTTTTCTCTCTCTCTACGCCCTCTTCTCCTCCTCAGCTTCCGCCTCCTCTAATTCCATTCATTTCTACAGCTTTGACCCGATCTCCTCCGACTGGCATCCCCTCCCCCCAACCCCTCCCGACCCGCCTCTCCGCCTCGTCCTTCGCCACCCCTCTTTCACATCCCGCAACTTCCCCATCCAATCCGTCTCCGCATGCGGCAACCTAATCCTCCTCGCAGCCACCACCCATAACTTTCTCCACGCTCTCCCTCGCCCTCTGGTCTTCGACCCAGTTACCAGAAACTGGACCTTCGGTCCCCCATTCACCACGCCACGCCGCTGGTGTGTGGTCGGAGTGCTAAGTGGAGTGGTCTACATGGCGAGTGGAATCGGGTCCCATTTCTCTATGGATGTTGCGCGTTCAGTAGAGAAGTGGGACTTAAGCAAATGGAAAAAGTATCAAGTTAATTCGAATGGTTGGGAGAAAGTGAGCGGGCTTCGAGATGGGAGATTCAGCAGAGATGCGATCGACGCTGTGGGATGGAGAGGAAAGCTCTGCATGGTCAATGTTAAGGGACACGCTGCAAAAGAGGGAGCTGTCTACGATGCGGAGAAGGACACGTGGCAAGACATGCCGGAAGGGATGATTATTGGGTGGAGAGGACCGGTGGCAGCCATGGACGAGGACGTGATGTACGTGGTGGACGAGGCCAACGGTGCTTTGAGAAAATACGATCCCAACAAGGATGTGTGGGAAGAGATATTCGAGTCCGAGAGGCTGAGAGGAGCTGATCAGATCGCTGCCGGAGGTGGAAGAGTTTGCGTTGTTTGTGGTGGTGAGATATTTGTGGTGGACGTGTCGGCGGCGACGCCGAGGCTTTGGGCTGTAGAAACGCCTTCGGGGCTAGAGGCGCTGGCGATTCATATCTTGCCGAGAATGAGCAATGCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0008150 GO:0009628 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:0050896 GO:0080167 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_6

Protein Analysis

378

Amino Acids

41.53

Weight (kDa)

6.37

Isoelectric Point (pI)

54.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 148 - 345 1.4e-10 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31350 AT1G31350
fragaria_vesca FvH4_5g24190
malus_domestica MD06G1017400.v1.1 MD06G1017500.v1.1 MD07G1079500.v1.1
prunus_persica Prupe.2G102300_v2.0.a1 Prupe.2G102400_v2.0.a1 Prupe.5G018500_v2.0.a1
pyrus_communis pycom06g01420
rosa_chinensis RchiOBHm_Chr7g0213971
rosa_laevigata RLG00000002765
rosa_multiflora Rmu_sc0000328.1_g000002
rosa_roxburghii Rroxscaffold_3G00245610
rosa_rugosa Rorug07G0145100
rosa_samantha Rh7AG278400 Rh7BG271900 Rh7CG297400 Rh7DG283900
rosa_wichuraiana Rw7G023950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 168
AccB7I CCANNNNNTGG 1 cut(s) 483
AccBSI CCGCTC 1 cut(s) 672
AccI GTMKAC 2 cut(s) 552, 780
AccII CGCG 1 cut(s) 598
AclWI GGATC 1 cut(s) 908
AcvI CACGTG 1 cut(s) 801
AcyI GRCGYC 2 cut(s) 169, 1049
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 2 cut(s) 291, 483
AflII CTTAAG 1 cut(s) 619
AflIII ACRYGT 2 cut(s) 798, 1035
AgeI ACCGGT 1 cut(s) 844
AhdI GACNNNNNGTC 1 cut(s) 492
AjiI CACGTC 2 cut(s) 868, 1036
AjnI CCWGG 1 cut(s) 88
AluBI AGCT 5 cut(s) 236, 270, 733, 776, 968
AluI AGCT 5 cut(s) 236, 270, 733, 776, 968
Alw26I GTCTC 1 cut(s) 391
AlwI GGATC 1 cut(s) 908
AlwNI CAGNNNCTG 1 cut(s) 483
AoxI GGCC 1 cut(s) 888
ApeKI GCWGC 4 cut(s) 416, 761, 851, 980
AsiGI ACCGGT 1 cut(s) 844
AspLEI GCGC 3 cut(s) 171, 598, 1093
AspS9I GGNCC 4 cut(s) 486, 494, 573, 842
AsuHPI GGTGA 2 cut(s) 496, 1028
AsuII TTCGAA 1 cut(s) 650
AvaII GGWCC 4 cut(s) 486, 494, 573, 842
BanI GGYRCC 1 cut(s) 168
BbrPI CACGTG 1 cut(s) 801
BbsI GAAGAC 1 cut(s) 454
BbvCI CCTCAGC 1 cut(s) 232
BbvI GCAGC 4 cut(s) 428, 748, 863, 967
BccI CCATC 4 cut(s) 141, 383, 677, 714
BciT130I CCWGG 1 cut(s) 90
BclI TGATCA 1 cut(s) 970
BcoDI GTCTC 1 cut(s) 391
BfaI CTAG 1 cut(s) 1085
BfmI CTRYAG 2 cut(s) 265, 1065
BfoI RGCGCY 2 cut(s) 172, 1094
BfrI CTTAAG 1 cut(s) 619
BglI GCCNNNNNGGC 1 cut(s) 1081
BisI GCNGC 8 cut(s) 172, 397, 417, 517, 762, 852, 981, 1044
BlsI GCNGC 8 cut(s) 173, 398, 418, 518, 763, 853, 982, 1045
Bme1390I CCNGG 1 cut(s) 90
Bme18I GGWCC 4 cut(s) 486, 494, 573, 842
BmeRI GACNNNNNGTC 1 cut(s) 492
BmgBI CACGTC 2 cut(s) 868, 1036
BmgT120I GGNCC 4 cut(s) 486, 494, 573, 842
BmiI GGNNCC 4 cut(s) 170, 496, 574, 575
BmrFI CCNGG 1 cut(s) 90
BmrI ACTGGG 1 cut(s) 464
BmsI GCATC 3 cut(s) 304, 691, 775
BmuI ACTGGG 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 454
BplI GAGNNNNNCTC 2 cut(s) 717, 749
Bpu10I CCTNAGC 1 cut(s) 232
Bpu14I TTCGAA 1 cut(s) 650
BsaAI YACGTR 2 cut(s) 801, 877
BsaBI GATNNNNATC 1 cut(s) 1103
BsaHI GRCGYC 2 cut(s) 169, 1049
BsaJI CCNNGG 2 cut(s) 855, 1052
BsaWI WCCGGW 1 cut(s) 844
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 266, 296
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 483
Bse118I RCCGGY 1 cut(s) 844
Bse1I ACTGG 3 cut(s) 296, 470, 488
Bse3DI GCAATG 2 cut(s) 12, 1129
Bse8I GATNNNNATC 1 cut(s) 1103
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 855, 1052
BseGI GGATG 8 cut(s) 133, 295, 359, 375, 595, 725, 828, 931
BseJI GATNNNNATC 1 cut(s) 1103
BseLI CCNNNNNNNGG 2 cut(s) 291, 483
BseMI GCAATG 2 cut(s) 12, 1129
BseMII CTCAG 3 cut(s) 51, 246, 950
BseNI ACTGG 3 cut(s) 296, 470, 488
BseRI GAGGAG 8 cut(s) 175, 178, 217, 220, 235, 274, 401, 978
BseXI GCAGC 4 cut(s) 428, 748, 863, 967
Bsh1236I CGCG 1 cut(s) 598
Bsh1285I CGRYCG 1 cut(s) 708
BshFI GGCC 1 cut(s) 890
BshNI GGYRCC 1 cut(s) 168
BshTI ACCGGT 1 cut(s) 844
BsiEI CGRYCG 1 cut(s) 708
BsiSI CCGG 4 cut(s) 98, 815, 845, 984
BslFI GGGAC 4 cut(s) 480, 559, 629, 768
BslI CCNNNNNNNGG 2 cut(s) 291, 483
BsmAI GTCTC 1 cut(s) 391
BsmBI CGTCTC 1 cut(s) 391
BsmFI GGGAC 4 cut(s) 480, 559, 629, 768
BsnI GGCC 1 cut(s) 890
Bsp119I TTCGAA 1 cut(s) 650
Bsp143I GATC 5 cut(s) 279, 705, 913, 970, 975
Bsp19I CCATGG 1 cut(s) 855
BspANI GGCC 1 cut(s) 890
BspCNI CTCAG 3 cut(s) 50, 245, 951
BspFNI CGCG 1 cut(s) 598
BspLI GGNNCC 4 cut(s) 170, 496, 574, 575
BspPI GGATC 1 cut(s) 908
BspT104I TTCGAA 1 cut(s) 650
BspT107I GGYRCC 1 cut(s) 168
BspTI CTTAAG 1 cut(s) 619
BsrBI CCGCTC 1 cut(s) 672
BsrDI GCAATG 2 cut(s) 12, 1129
BsrFI RCCGGY 1 cut(s) 844
BsrI ACTGG 3 cut(s) 296, 470, 488
BssAI RCCGGY 1 cut(s) 844
BssECI CCNNGG 2 cut(s) 855, 1052
BssMI GATC 5 cut(s) 279, 705, 913, 970, 975
BssNI GRCGYC 2 cut(s) 169, 1049
BssT1I CCWWGG 1 cut(s) 855
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 1 cut(s) 896
Bst6I CTCTTC 3 cut(s) 227, 930, 988
BstACI GRCGYC 2 cut(s) 169, 1049
BstAFI CTTAAG 1 cut(s) 619
BstAPI GCANNNNNTGC 1 cut(s) 811
BstBAI YACGTR 2 cut(s) 801, 877
BstBI TTCGAA 1 cut(s) 650
BstC8I GCNNGC 3 cut(s) 394, 674, 1095
BstDEI CTNAG 4 cut(s) 37, 232, 539, 959
BstDSI CCRYGG 1 cut(s) 855
BstF5I GGATG 8 cut(s) 133, 295, 359, 375, 595, 725, 828, 931
BstFNI CGCG 1 cut(s) 598
BstH2I RGCGCY 2 cut(s) 172, 1094
BstHHI GCGC 3 cut(s) 171, 598, 1093
BstKTI GATC 5 cut(s) 282, 708, 916, 973, 978
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 5 cut(s) 279, 705, 913, 970, 975
BstMCI CGRYCG 1 cut(s) 708
BstMWI GCNNNNNNNGC 7 cut(s) 85, 94, 710, 811, 896, 1081, 1090
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 2 cut(s) 396, 814
BstSCI CCNGG 1 cut(s) 88
BstSFI CTRYAG 2 cut(s) 265, 1065
BstUI CGCG 1 cut(s) 598
BstV1I GCAGC 4 cut(s) 428, 748, 863, 967
BstV2I GAAGAC 1 cut(s) 454
BstXI CCANNNNNNTGG 1 cut(s) 519
BsuRI GGCC 1 cut(s) 890
BtgI CCRYGG 1 cut(s) 855
BtrI CACGTC 2 cut(s) 868, 1036
BtsCI GGATG 8 cut(s) 133, 295, 359, 375, 595, 725, 828, 931
Cac8I GCNNGC 3 cut(s) 394, 674, 1095
CaiI CAGNNNCTG 1 cut(s) 483
CfoI GCGC 3 cut(s) 171, 598, 1093
Cfr10I RCCGGY 1 cut(s) 844
Cfr13I GGNCC 4 cut(s) 486, 494, 573, 842
CseI GACGC 2 cut(s) 719, 1057
Csp6I GTAC 1 cut(s) 874
CspAI ACCGGT 1 cut(s) 844
CviAII CATG 6 cut(s) 165, 393, 556, 739, 811, 856
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 4 cut(s) 37, 232, 539, 959
DinI GGCGCC 1 cut(s) 170
DpnI GATC 5 cut(s) 281, 707, 915, 972, 977
DpnII GATC 5 cut(s) 279, 705, 913, 970, 975
DriI GACNNNNNGTC 1 cut(s) 492
Eam1104I CTCTTC 3 cut(s) 227, 930, 988
Eam1105I GACNNNNNGTC 1 cut(s) 492
EarI CTCTTC 3 cut(s) 227, 930, 988
EciI GGCGGA 2 cut(s) 229, 320
Eco130I CCWWGG 1 cut(s) 855
Eco47I GGWCC 4 cut(s) 486, 494, 573, 842
Eco72I CACGTG 1 cut(s) 801
EcoO109I RGGNCCY 1 cut(s) 573
EcoRII CCWGG 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 855
EgeI GGCGCC 1 cut(s) 170
EheI GGCGCC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 855
Esp3I CGTCTC 1 cut(s) 391
FaeI CATG 6 cut(s) 168, 396, 559, 742, 814, 859
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 4 cut(s) 480, 559, 629, 768
FatI CATG 6 cut(s) 164, 392, 555, 738, 810, 855
FauI CCCGC 3 cut(s) 330, 371, 665
FbaI TGATCA 1 cut(s) 970
FblI GTMKAC 2 cut(s) 552, 780
Fnu4HI GCNGC 8 cut(s) 172, 397, 417, 517, 762, 852, 981, 1044
FokI GGATG 8 cut(s) 120, 282, 346, 362, 602, 732, 835, 938
Fsp4HI GCNGC 8 cut(s) 172, 397, 417, 517, 762, 852, 981, 1044
FspBI CTAG 1 cut(s) 1085
GlaI GCGC 3 cut(s) 170, 597, 1092
GluI GCNGC 8 cut(s) 172, 397, 417, 517, 762, 852, 981, 1044
HaeII RGCGCY 2 cut(s) 172, 1094
HaeIII GGCC 1 cut(s) 890
HapII CCGG 4 cut(s) 98, 815, 845, 984
HgaI GACGC 2 cut(s) 719, 1057
HhaI GCGC 3 cut(s) 171, 598, 1093
Hin1I GRCGYC 2 cut(s) 169, 1049
Hin1II CATG 6 cut(s) 168, 396, 559, 742, 814, 859
Hin6I GCGC 3 cut(s) 169, 596, 1091
HinP1I GCGC 3 cut(s) 169, 596, 1091
HinfI GANTC 5 cut(s) 55, 567, 690, 947, 1099
HpaII CCGG 4 cut(s) 98, 815, 845, 984
HphI GGTGA 2 cut(s) 496, 1028
Hpy166II GTNNAC 4 cut(s) 553, 781, 883, 1033
Hpy188I TCNGA 6 cut(s) 13, 40, 289, 533, 952, 975
Hpy188III TCNNGA 2 cut(s) 317, 680
Hpy8I GTNNAC 4 cut(s) 553, 781, 883, 1033
Hpy99I CGWCG 3 cut(s) 68, 713, 1051
HpyAV CCTTC 7 cut(s) 147, 202, 350, 499, 787, 812, 1086
HpyCH4III ACNGT 1 cut(s) 896
HpyCH4IV ACGT 4 cut(s) 800, 867, 876, 1035
HpyCH4V TGCA 3 cut(s) 159, 738, 764
HpyF10VI GCNNNNNNNGC 7 cut(s) 85, 94, 710, 811, 896, 1081, 1090
HpyF3I CTNAG 4 cut(s) 37, 232, 539, 959
HpySE526I ACGT 4 cut(s) 800, 867, 876, 1035
Hsp92I GRCGYC 2 cut(s) 169, 1049
Hsp92II CATG 6 cut(s) 168, 396, 559, 742, 814, 859
HspAI GCGC 3 cut(s) 169, 596, 1091
KasI GGCGCC 1 cut(s) 168
KflI GGGWCCC 1 cut(s) 573
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 5 cut(s) 279, 705, 913, 970, 975
LmnI GCTCC 2 cut(s) 773, 965
Lsp1109I GCAGC 4 cut(s) 428, 748, 863, 967
LweI GCATC 3 cut(s) 304, 691, 775
MaeI CTAG 1 cut(s) 1085
MaeII ACGT 4 cut(s) 800, 867, 876, 1035
MaeIII GTNAC 1 cut(s) 472
MalI GATC 5 cut(s) 281, 707, 915, 972, 977
MbiI CCGCTC 1 cut(s) 672
MboI GATC 5 cut(s) 279, 705, 913, 970, 975
MboII GAAGA 4 cut(s) 214, 454, 947, 1005
MluCI AATT 2 cut(s) 250, 646
Mly113I GGCGCC 1 cut(s) 169
MlyI GAGTC 2 cut(s) 64, 956
MmeI TCCRAC 2 cut(s) 312, 511
MseI TTAA 3 cut(s) 620, 645, 750
MspA1I CMGCKG 1 cut(s) 519
MspCI CTTAAG 1 cut(s) 619
MspI CCGG 4 cut(s) 98, 815, 845, 984
MspR9I CCNGG 1 cut(s) 90
MvaI CCWGG 1 cut(s) 90
MvnI CGCG 1 cut(s) 598
MwoI GCNNNNNNNGC 7 cut(s) 85, 94, 710, 811, 896, 1081, 1090
NarI GGCGCC 1 cut(s) 169
NcoI CCATGG 1 cut(s) 855
NdeII GATC 5 cut(s) 279, 705, 913, 970, 975
NlaIII CATG 6 cut(s) 168, 396, 559, 742, 814, 859
NlaIV GGNNCC 4 cut(s) 170, 496, 574, 575
NmeAIII GCCGAG 1 cut(s) 1077
NspI RCATGY 2 cut(s) 396, 814
NspV TTCGAA 1 cut(s) 650
PaeI GCATGC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 2 cut(s) 873, 882
PfeI GAWTC 3 cut(s) 567, 690, 1099
PflMI CCANNNNNTGG 1 cut(s) 483
PinAI ACCGGT 1 cut(s) 844
PkrI GCNGC 8 cut(s) 173, 398, 418, 518, 763, 853, 982, 1045
Ple19I CGATCG 1 cut(s) 708
PleI GAGTC 2 cut(s) 63, 955
PluTI GGCGCC 1 cut(s) 172
PmaCI CACGTG 1 cut(s) 801
PmlI CACGTG 1 cut(s) 801
PpsI GAGTC 2 cut(s) 63, 955
Ppu21I YACGTR 2 cut(s) 801, 877
PpuMI RGGWCCY 1 cut(s) 573
Psp5II RGGWCCY 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 88
PspCI CACGTG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 4 cut(s) 170, 496, 574, 575
PspPI GGNCC 4 cut(s) 486, 494, 573, 842
PspPPI RGGWCCY 1 cut(s) 573
PstNI CAGNNNCTG 1 cut(s) 483
PvuI CGATCG 1 cut(s) 708
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
SaqAI TTAA 3 cut(s) 620, 645, 750
SatI GCNGC 8 cut(s) 172, 397, 417, 517, 762, 852, 981, 1044
Sau3AI GATC 5 cut(s) 279, 705, 913, 970, 975
Sau96I GGNCC 4 cut(s) 486, 494, 573, 842
SchI GAGTC 2 cut(s) 64, 956
ScrFI CCNGG 1 cut(s) 90
SfaNI GCATC 3 cut(s) 304, 691, 775
SfcI CTRYAG 2 cut(s) 265, 1065
SfoI GGCGCC 1 cut(s) 170
SfuI TTCGAA 1 cut(s) 650
SinI GGWCC 4 cut(s) 486, 494, 573, 842
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
SphI GCATGC 1 cut(s) 396
Sse9I AATT 2 cut(s) 250, 646
SspDI GGCGCC 1 cut(s) 168
SspMI CTAG 1 cut(s) 1085
StyD4I CCNGG 1 cut(s) 88
StyI CCWWGG 1 cut(s) 855
TaaI ACNGT 1 cut(s) 896
TaiI ACGT 4 cut(s) 803, 870, 879, 1038
TaqI TCGA 5 cut(s) 465, 650, 679, 708, 945
TaqII GACCGA 1 cut(s) 482
TasI AATT 2 cut(s) 250, 646
TauI GCSGC 4 cut(s) 174, 399, 519, 1046
TfiI GAWTC 3 cut(s) 567, 690, 1099
Tru1I TTAA 3 cut(s) 620, 645, 750
Tru9I TTAA 3 cut(s) 620, 645, 750
TseI GCWGC 4 cut(s) 416, 761, 851, 980
TspDTI ATGAA 2 cut(s) 248, 1091
TspGWI ACGGA 3 cut(s) 67, 142, 373
Van91I CCANNNNNTGG 1 cut(s) 483
Vha464I CTTAAG 1 cut(s) 619
VpaK11BI GGWCC 4 cut(s) 486, 494, 573, 842
XceI RCATGY 2 cut(s) 396, 814
XmiI GTMKAC 2 cut(s) 552, 780
XspI CTAG 1 cut(s) 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.