MD07G1079500.v1.1

F-box kelch-repeat protein SKIP25-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011373
Physical Location & Seq
Forward (+)
7657879 .. 7659024
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1079500.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGGATCAAGACATACCAAAACCAAAACGCAACAAAAACACACAGAACTCTTCCAACGAGTGTTCGTTGTTGCCAGGCTTACCCAACCACCTTGCACAGCTCTGCCTTACAAGGATCCAACCTTCAACTCTCTACTTTGTTTGCCGTTCATGGCGGCGCCTCATTTACTCTCCTTCATTCCCTCCTTTCTTCGCTCTCTATGCCATTCTCTCCCCTCCACCTCCCCTCAACTGCAATTCATCAATTGAGTTCTACTCACTAGATCCCCTATCAAACAATTGGACATCTCTCCCTCCACCTCCTTCAGACCCTCCTCTTCACCTCCTCCGCCGCCACCCTTCCTTCCTCTCCCGAAAAATCCCCATCCAATCACTATCCGTCTCTGGCAGTCTTGTCCTCGTCGCAGCCACTAATTACAATTTCACTCCAGCACTGCCTCGTCCTCTCGTCTTCCGACCATTGTCCAACCAATGGTCATTTGGCCCTCCGCTAGCAGAGCCTCGCCGGTGGTGTGCGACGGGGACAGTAGGGGGAAAAGTCTACGTTGCAAGTGGGATTGGATCAAATTACAGAGGCGACGTGGCGAGGTCTATGGAGGAGTGGGACATGAAGAAGAAGAAGGAGGCATCTTCATCATGGGTTAAGAAGGCGGGGTTGAAAGATGGGAGGTTTAGTAGGGAGGCTGTGGAGGCAATTGGGTATAGAGGGAAGCTTTGGATGGTGAATGTGAAGGGCAATGCAAGAAAAGAAGGAGCTGTTTATGACGTGGAAAATGACACATGGCAAGACATGCCTCAAGGCATGATTGGTGGTTGGAATGGGCCTGCTGCTGCATCCATCAATGACGACGACGACGATGATCATGATCATGATGTGATGTATGTGGTGGATGAGAGTACAGGCGTGCTGAGCAAGTATGATGATGAGAACGACTGCTGGGAAATGGTGGTGGAGTCGGATGTGTTAAGAGAAGCTGAGCAAGTTTGTGCAGGGAGAGGGAGAGTTTGTGTGGTTTGTGCTAACGAGAAAAGGATTGCGGTGGTGGATGTGGTGGCGCCGCAGCCGAGGATTTGGGTGGTGGAGCCGCCGCCGATGCTGCAAGTTGTGGCGGTACACATTTTGCCTAGGATGTGTAGGTCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_6

Protein Analysis

382

Amino Acids

42.55

Weight (kDa)

6.61

Isoelectric Point (pI)

51.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 124 - 359 4.9e-11 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31350 AT1G31350
fragaria_vesca FvH4_5g24190
malus_domestica MD06G1017400.v1.1 MD06G1017500.v1.1 MD07G1079500.v1.1
prunus_persica Prupe.2G102300_v2.0.a1 Prupe.2G102400_v2.0.a1 Prupe.5G018500_v2.0.a1
pyrus_communis pycom06g01420
rosa_chinensis RchiOBHm_Chr7g0213971
rosa_laevigata RLG00000002765
rosa_multiflora Rmu_sc0000328.1_g000002
rosa_roxburghii Rroxscaffold_3G00245610
rosa_rugosa Rorug07G0145100
rosa_samantha Rh7AG278400 Rh7BG271900 Rh7CG297400 Rh7DG283900
rosa_wichuraiana Rw7G023950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 156, 1054
AccB7I CCANNNNNTGG 1 cut(s) 471
AccI GTMKAC 1 cut(s) 540
AclWI GGATC 5 cut(s) 12, 109, 122, 257, 568
AcuI CTGAAG 1 cut(s) 288
AcyI GRCGYC 2 cut(s) 157, 1055
AfaI GTAC 2 cut(s) 898, 1113
AfiI CCNNNNNNNGG 1 cut(s) 471
AgsI TTSAA 2 cut(s) 126, 658
AjiI CACGTC 2 cut(s) 580, 766
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 4 cut(s) 100, 712, 755, 974
AluI AGCT 4 cut(s) 100, 712, 755, 974
Alw26I GTCTC 1 cut(s) 385
AlwI GGATC 5 cut(s) 12, 109, 122, 257, 568
AoxI GGCC 2 cut(s) 481, 821
ApeKI GCWGC 5 cut(s) 404, 827, 830, 1060, 1096
AspA2I CCTAGG 1 cut(s) 1124
AspLEI GCGC 2 cut(s) 159, 1057
AspS9I GGNCC 2 cut(s) 482, 821
AsuHPI GGTGA 2 cut(s) 311, 733
AsuNHI GCTAGC 1 cut(s) 490
AvrII CCTAGG 1 cut(s) 1124
BamHI GGATCC 1 cut(s) 114
BanI GGYRCC 2 cut(s) 156, 1054
BbsI GAAGAC 1 cut(s) 442
BbvI GCAGC 5 cut(s) 416, 814, 817, 1072, 1083
BccI CCATC 4 cut(s) 371, 656, 712, 845
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 1 cut(s) 75
BclI TGATCA 2 cut(s) 859, 865
BcoDI GTCTC 1 cut(s) 385
BfaI CTAG 3 cut(s) 260, 491, 1125
BfoI RGCGCY 2 cut(s) 160, 1058
BlnI CCTAGG 1 cut(s) 1124
BlpI GCTNAGC 2 cut(s) 908, 975
Bme1390I CCNGG 1 cut(s) 75
BmgBI CACGTC 2 cut(s) 580, 766
BmgT120I GGNCC 2 cut(s) 482, 821
BmiI GGNNCC 4 cut(s) 116, 158, 1056, 1083
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 3 cut(s) 635, 842, 1083
BmtI GCTAGC 1 cut(s) 494
BoxI GACNNNNGTC 1 cut(s) 460
BpiI GAAGAC 1 cut(s) 442
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 1 cut(s) 411
Bpu1102I GCTNAGC 2 cut(s) 908, 975
BpuEI CTTGAG 1 cut(s) 780
BsaBI GATNNNNATC 1 cut(s) 864
BsaHI GRCGYC 2 cut(s) 157, 1055
BsaJI CCNNGG 2 cut(s) 1064, 1124
BsaXI ACNNNNNCTCC 4 cut(s) 658, 688, 991, 1021
Bsc4I CCNNNNNNNGG 1 cut(s) 471
Bse118I RCCGGY 1 cut(s) 504
Bse3DI GCAATG 1 cut(s) 742
Bse8I GATNNNNATC 1 cut(s) 864
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 1064, 1124
BseGI GGATG 7 cut(s) 363, 723, 833, 895, 964, 1051, 1134
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 1 cut(s) 471
BseMI GCAATG 1 cut(s) 742
BseMII CTCAG 2 cut(s) 899, 966
BseRI GAGGAG 3 cut(s) 303, 314, 611
BseXI GCAGC 5 cut(s) 416, 814, 817, 1072, 1083
BseYI CCCAGC 1 cut(s) 936
BsgI GTGCAG 1 cut(s) 1008
BshFI GGCC 2 cut(s) 483, 823
BshNI GGYRCC 2 cut(s) 156, 1054
BsiSI CCGG 1 cut(s) 505
BslFI GGGAC 2 cut(s) 535, 617
BslI CCNNNNNNNGG 1 cut(s) 471
BsmAI GTCTC 1 cut(s) 385
BsmBI CGTCTC 1 cut(s) 385
BsmFI GGGAC 2 cut(s) 535, 617
BsnI GGCC 2 cut(s) 483, 823
Bsp143I GATC 6 cut(s) 4, 114, 262, 560, 859, 865
Bsp1720I GCTNAGC 2 cut(s) 908, 975
BspANI GGCC 2 cut(s) 483, 823
BspCNI CTCAG 2 cut(s) 900, 967
BspHI TCATGA 2 cut(s) 862, 868
BspLI GGNNCC 4 cut(s) 116, 158, 1056, 1083
BspOI GCTAGC 1 cut(s) 494
BspPI GGATC 5 cut(s) 12, 109, 122, 257, 568
BspT107I GGYRCC 2 cut(s) 156, 1054
BsrDI GCAATG 1 cut(s) 742
BsrFI RCCGGY 1 cut(s) 504
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 2 cut(s) 1064, 1124
BssMI GATC 6 cut(s) 4, 114, 262, 560, 859, 865
BssNI GRCGYC 2 cut(s) 157, 1055
BssT1I CCWWGG 1 cut(s) 1124
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 1 cut(s) 526
Bst6I CTCTTC 2 cut(s) 55, 321
BstACI GRCGYC 2 cut(s) 157, 1055
BstAPI GCANNNNNTGC 1 cut(s) 790
BstC8I GCNNGC 3 cut(s) 492, 825, 905
BstDEI CTNAG 2 cut(s) 908, 975
BstF5I GGATG 7 cut(s) 363, 723, 833, 895, 964, 1051, 1134
BstH2I RGCGCY 2 cut(s) 160, 1058
BstHHI GCGC 2 cut(s) 159, 1057
BstKTI GATC 6 cut(s) 7, 117, 265, 563, 862, 868
BstMAI GTCTC 1 cut(s) 385
BstMBI GATC 6 cut(s) 4, 114, 262, 560, 859, 865
BstMWI GCNNNNNNNGC 7 cut(s) 200, 496, 689, 790, 909, 1093, 1096
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 1 cut(s) 793
BstPAI GACNNNNGTC 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 73
BstV1I GCAGC 5 cut(s) 416, 814, 817, 1072, 1083
BstV2I GAAGAC 1 cut(s) 442
BstX2I RGATCY 2 cut(s) 114, 262
BstYI RGATCY 2 cut(s) 114, 262
BsuRI GGCC 2 cut(s) 483, 823
BtrI CACGTC 2 cut(s) 580, 766
BtsCI GGATG 7 cut(s) 363, 723, 833, 895, 964, 1051, 1134
BtsI GCAGTG 1 cut(s) 431
BtsIMutI CAGTG 1 cut(s) 431
Cac8I GCNNGC 3 cut(s) 492, 825, 905
CciI TCATGA 2 cut(s) 862, 868
CfoI GCGC 2 cut(s) 159, 1057
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 2 cut(s) 482, 821
Csp6I GTAC 2 cut(s) 897, 1112
CviAII CATG 8 cut(s) 150, 607, 636, 780, 790, 802, 863, 869
CviQI GTAC 2 cut(s) 897, 1112
DdeI CTNAG 2 cut(s) 908, 975
DinI GGCGCC 2 cut(s) 158, 1056
DpnI GATC 6 cut(s) 6, 116, 264, 562, 861, 867
DpnII GATC 6 cut(s) 4, 114, 262, 560, 859, 865
Eam1104I CTCTTC 2 cut(s) 55, 321
EarI CTCTTC 2 cut(s) 55, 321
EciI GGCGGA 1 cut(s) 317
Eco130I CCWWGG 1 cut(s) 1124
Eco57I CTGAAG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 1124
EgeI GGCGCC 2 cut(s) 158, 1056
EheI GGCGCC 2 cut(s) 158, 1056
ErhI CCWWGG 1 cut(s) 1124
Esp3I CGTCTC 1 cut(s) 385
FaeI CATG 8 cut(s) 153, 610, 639, 783, 793, 805, 866, 872
FaqI GGGAC 2 cut(s) 535, 617
FatI CATG 8 cut(s) 149, 606, 635, 779, 789, 801, 862, 868
FauI CCCGC 1 cut(s) 643
FbaI TGATCA 2 cut(s) 859, 865
FblI GTMKAC 1 cut(s) 540
FokI GGATG 7 cut(s) 350, 730, 820, 902, 971, 1058, 1141
FspBI CTAG 3 cut(s) 260, 491, 1125
GlaI GCGC 2 cut(s) 158, 1056
GsaI CCCAGC 1 cut(s) 940
GsuI CTGGAG 1 cut(s) 411
HaeII RGCGCY 2 cut(s) 160, 1058
HaeIII GGCC 2 cut(s) 483, 823
HapII CCGG 1 cut(s) 505
HhaI GCGC 2 cut(s) 159, 1057
Hin1I GRCGYC 2 cut(s) 157, 1055
Hin1II CATG 8 cut(s) 153, 610, 639, 783, 793, 805, 866, 872
Hin6I GCGC 2 cut(s) 157, 1055
HinP1I GCGC 2 cut(s) 157, 1055
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 1 cut(s) 953
HpaII CCGG 1 cut(s) 505
HphI GGTGA 2 cut(s) 311, 733
Hpy166II GTNNAC 2 cut(s) 541, 1114
Hpy188I TCNGA 4 cut(s) 307, 455, 958, 1141
Hpy188III TCNNGA 4 cut(s) 8, 351, 863, 869
Hpy8I GTNNAC 2 cut(s) 541, 1114
Hpy99I CGWCG 6 cut(s) 404, 520, 581, 851, 854, 857
HpyAV CCTTC 9 cut(s) 132, 183, 312, 348, 352, 613, 640, 724, 743
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4IV ACGT 3 cut(s) 543, 579, 765
HpyCH4V TGCA 7 cut(s) 95, 234, 548, 740, 833, 989, 1099
HpyF10VI GCNNNNNNNGC 7 cut(s) 200, 496, 689, 790, 909, 1093, 1096
HpyF3I CTNAG 2 cut(s) 908, 975
HpySE526I ACGT 3 cut(s) 543, 579, 765
Hsp92I GRCGYC 2 cut(s) 157, 1055
Hsp92II CATG 8 cut(s) 153, 610, 639, 783, 793, 805, 866, 872
HspAI GCGC 2 cut(s) 157, 1055
KasI GGCGCC 2 cut(s) 156, 1054
Ksp22I TGATCA 2 cut(s) 859, 865
Kzo9I GATC 6 cut(s) 4, 114, 262, 560, 859, 865
LmnI GCTCC 2 cut(s) 752, 1081
LpnPI CCDG 9 cut(s) 60, 87, 369, 441, 518, 837, 885, 922, 975
Lsp1109I GCAGC 5 cut(s) 416, 814, 817, 1072, 1083
LweI GCATC 3 cut(s) 635, 842, 1083
MaeI CTAG 3 cut(s) 260, 491, 1125
MaeII ACGT 3 cut(s) 543, 579, 765
MalI GATC 6 cut(s) 6, 116, 264, 562, 861, 867
MboI GATC 6 cut(s) 4, 114, 262, 560, 859, 865
MboII GAAGA 8 cut(s) 42, 181, 308, 442, 621, 622, 625, 628
MfeI CAATTG 3 cut(s) 243, 277, 693
MflI RGATCY 2 cut(s) 114, 262
MluCI AATT 7 cut(s) 235, 243, 277, 412, 418, 565, 693
Mly113I GGCGCC 2 cut(s) 157, 1055
MlyI GAGTC 1 cut(s) 962
MmeI TCCRAC 6 cut(s) 78, 142, 478, 489, 794, 936
MseI TTAA 2 cut(s) 642, 965
MslI CAYNNNNRTG 1 cut(s) 867
MspI CCGG 1 cut(s) 505
MspR9I CCNGG 1 cut(s) 75
MunI CAATTG 3 cut(s) 243, 277, 693
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 7 cut(s) 200, 496, 689, 790, 909, 1093, 1096
NarI GGCGCC 2 cut(s) 157, 1055
NdeII GATC 6 cut(s) 4, 114, 262, 560, 859, 865
NheI GCTAGC 1 cut(s) 490
NlaIII CATG 8 cut(s) 153, 610, 639, 783, 793, 805, 866, 872
NlaIV GGNNCC 4 cut(s) 116, 158, 1056, 1083
NmeAIII GCCGAG 1 cut(s) 1089
NspI RCATGY 1 cut(s) 793
PagI TCATGA 2 cut(s) 862, 868
PcsI WCGNNNNNNNCGW 1 cut(s) 852
PflMI CCANNNNNTGG 1 cut(s) 471
PleI GAGTC 1 cut(s) 961
PluTI GGCGCC 2 cut(s) 160, 1058
PpsI GAGTC 1 cut(s) 961
PshAI GACNNNNGTC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 73
PspFI CCCAGC 1 cut(s) 936
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 4 cut(s) 116, 158, 1056, 1083
PspPI GGNCC 2 cut(s) 482, 821
PsuI RGATCY 2 cut(s) 114, 262
RsaI GTAC 2 cut(s) 898, 1113
RsaNI GTAC 2 cut(s) 897, 1112
RseI CAYNNNNRTG 1 cut(s) 867
SaqAI TTAA 2 cut(s) 642, 965
Sau3AI GATC 6 cut(s) 4, 114, 262, 560, 859, 865
Sau96I GGNCC 2 cut(s) 482, 821
SchI GAGTC 1 cut(s) 962
ScrFI CCNGG 1 cut(s) 75
SfaNI GCATC 3 cut(s) 635, 842, 1083
SfoI GGCGCC 2 cut(s) 158, 1056
SgrAI CRCCGGYG 1 cut(s) 504
SmiMI CAYNNNNRTG 1 cut(s) 867
SmlI CTYRAG 1 cut(s) 795
SmoI CTYRAG 1 cut(s) 795
Sse9I AATT 7 cut(s) 235, 243, 277, 412, 418, 565, 693
SspDI GGCGCC 2 cut(s) 156, 1054
SspMI CTAG 3 cut(s) 260, 491, 1125
StyD4I CCNGG 1 cut(s) 73
StyI CCWWGG 1 cut(s) 1124
TaaI ACNGT 1 cut(s) 526
TaiI ACGT 3 cut(s) 546, 582, 768
TasI AATT 7 cut(s) 235, 243, 277, 412, 418, 565, 693
TatI WGTACW 1 cut(s) 896
TauI GCSGC 5 cut(s) 157, 333, 1060, 1087, 1090
Tru1I TTAA 2 cut(s) 642, 965
Tru9I TTAA 2 cut(s) 642, 965
TscAI CASTG 1 cut(s) 438
TseI GCWGC 5 cut(s) 404, 827, 830, 1060, 1096
TspDTI ATGAA 5 cut(s) 138, 165, 228, 621, 623
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 1 cut(s) 438
Van91I CCANNNNNTGG 1 cut(s) 471
XceI RCATGY 1 cut(s) 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmaJI CCTAGG 1 cut(s) 1124
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 3 cut(s) 260, 491, 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.