Prupe.2G102300_v2.0.a1

F-box kelch-repeat protein SKIP25-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0011373
Physical Location & Seq
Forward (+)
15805654 .. 15807397
1744 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G102300.1

Sequence Viewer

Length: 1158 bp
ATGGATCAAGAAGCCATACCAAAACCAAAACACTGTCGAACTCAAGCCTCTTCCGATGAGACTTTGCTGTTGCCAGGCTTACCAAACCACCTTGCACAGCTCTGCCTTGCAAAAATCAACCCTTCCGTTCTCTTCTCTGTTTGCCGCTCATGGCGGCGCCTTATCTACTCTCCTTCCTTCCCTCCTTTTTTCTCTCTCTACGCCATTCTCTCTCCACCACCACCTCATCACCACCACCACAACCCCAATTCCCCCATTCAGTTCTCCTCACTAGACCCCATATCAAACACATGGACATCTCTCCCTCCTCCTCCCTCCTCCCCTCCTCTCCAACTCCTCCACTGCCACCCGTCCTTCCTCTCCCGAAAACTACCGATCCAATGGTTGTCCGTCTCCGGCCGCCTTGTCCTCGTTGCTGCCACTACCCACCAGTTCACACCAGCGATGCCTCGTCCTCTTGTTTTCCAACCACTGTCCAACCAGTGGTCCTTCGGCCCCCCGCTGCCCGAGCCGCGCCGCTGGTGTGGGGTCGGGACTGTGGGTGGAAAAGTGTACGTGGCTAGTGGGATTGGAACAACTTATAGAGGAGATGTTGCGAGGTCGATGGAAGAATGGGACACTAAGAGAAAGGAGGCTTCTTCGTCATGGGTTAAGAAGGCATGCCTTAAAGATGGAAGGTTTAGCAGGGAGGATGTGGAGGCAATTGGGTACAGAGGGAAGCTATGGATGGTGAATTCCAAGGGCAATGCAGTCAAAGAAGGGGTTGTCTATGATGTGCAGAAGGACACGTGGCAAGTCATGCCTCAAGGCATGCTCGGTGGATGGAATGGGCCAGCTGCTGCAACCATCAATGATGATGGTCATGATGACCATGATGATGATGATGTGATGTATGTGGTGGATGAGAGAAAAGGAGTATTGAACAAGTATGTTGATGAAAATGACTGCTGGGAAATGGTGATGGAGTCGGTTGTGTTAAGAGAGGCTGAGCAAGTTTGTGCCGGGAGGGGGAGAGTTTGCGTGGTTTGTGCTAACGGAAGAAGGATTGTGGTGGTAGATGTCGTGGCGACACCACCGCGAATTTGGGTGGTGGAGCCGCCGCCGATGTTGGAAGTTGTGGCTGTACACATTTTGCCTAGGATGTGTAGGACGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_6

Protein Analysis

386

Amino Acids

42.96

Weight (kDa)

7.6

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31350 AT1G31350
fragaria_vesca FvH4_5g24190
malus_domestica MD06G1017400.v1.1 MD06G1017500.v1.1 MD07G1079500.v1.1
prunus_persica Prupe.2G102300_v2.0.a1 Prupe.2G102400_v2.0.a1 Prupe.5G018500_v2.0.a1
pyrus_communis pycom06g01420
rosa_chinensis RchiOBHm_Chr7g0213971
rosa_laevigata RLG00000002765
rosa_multiflora Rmu_sc0000328.1_g000002
rosa_roxburghii Rroxscaffold_3G00245610
rosa_rugosa Rorug07G0145100
rosa_samantha Rh7AG278400 Rh7BG271900 Rh7CG297400 Rh7DG283900
rosa_wichuraiana Rw7G023950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 156
AccB7I CCANNNNNTGG 1 cut(s) 483
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 2 cut(s) 514, 1078
AciI CCGC 9 cut(s) 145, 154, 400, 500, 512, 517, 1076, 1097, 1100
AclWI GGATC 2 cut(s) 12, 370
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 2 cut(s) 733, 1080
AcvI CACGTG 1 cut(s) 789
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 3 cut(s) 554, 710, 1125
AfiI CCNNNNNNNGG 2 cut(s) 483, 1008
AflIII ACRYGT 1 cut(s) 786
AgsI TTSAA 1 cut(s) 922
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 3 cut(s) 100, 721, 836
AluI AGCT 3 cut(s) 100, 721, 836
Alw26I GTCTC 2 cut(s) 53, 397
AlwI GGATC 2 cut(s) 12, 370
AlwNI CAGNNNCTG 1 cut(s) 839
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 3 cut(s) 397, 493, 830
ApeKI GCWGC 4 cut(s) 416, 502, 836, 839
ApoI RAATTY 2 cut(s) 733, 1080
AspA2I CCTAGG 1 cut(s) 1136
AspLEI GCGC 2 cut(s) 159, 516
AspS9I GGNCC 3 cut(s) 486, 494, 830
AsuC2I CCSGG 1 cut(s) 1003
AsuHPI GGTGA 3 cut(s) 221, 742, 970
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 486
AvrII CCTAGG 1 cut(s) 1136
BanI GGYRCC 1 cut(s) 156
BbrPI CACGTG 1 cut(s) 789
BbvI GCAGC 4 cut(s) 403, 489, 823, 826
BccI CCATC 7 cut(s) 598, 665, 721, 816, 851, 854, 955
BciT130I CCWGG 1 cut(s) 75
BcnI CCSGG 1 cut(s) 1003
BcoDI GTCTC 2 cut(s) 53, 397
BfaI CTAG 3 cut(s) 272, 561, 1137
BfoI RGCGCY 1 cut(s) 160
BlnI CCTAGG 1 cut(s) 1136
BlpI GCTNAGC 1 cut(s) 987
Bme1390I CCNGG 2 cut(s) 75, 1003
Bme18I GGWCC 1 cut(s) 486
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 3 cut(s) 486, 494, 830
BmiI GGNNCC 3 cut(s) 158, 496, 1095
BmrFI CCNGG 2 cut(s) 75, 1003
BmsI GCATC 1 cut(s) 435
Bpu1102I GCTNAGC 1 cut(s) 987
BpuEI CTTGAG 2 cut(s) 27, 789
BpuMI CCSGG 1 cut(s) 1003
BsaAI YACGTR 2 cut(s) 556, 789
BsaHI GRCGYC 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 738, 1136
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 1008
Bse1I ACTGG 2 cut(s) 430, 481
Bse3DI GCAATG 1 cut(s) 751
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 738, 1136
BseGI GGATG 5 cut(s) 697, 732, 827, 907, 1146
BseLI CCNNNNNNNGG 2 cut(s) 483, 1008
BseMI GCAATG 1 cut(s) 751
BseMII CTCAG 1 cut(s) 978
BseNI ACTGG 2 cut(s) 430, 481
BseRI GAGGAG 7 cut(s) 256, 297, 300, 307, 315, 326, 600
BseX3I CGGCCG 1 cut(s) 397
BseXI GCAGC 4 cut(s) 403, 489, 823, 826
BseYI CCCAGC 1 cut(s) 948
BsgI GTGCAG 1 cut(s) 797
Bsh1236I CGCG 2 cut(s) 514, 1078
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 3 cut(s) 399, 495, 832
BshNI GGYRCC 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 2 cut(s) 396, 1002
BslFI GGGAC 2 cut(s) 547, 629
BslI CCNNNNNNNGG 2 cut(s) 483, 1008
BsmAI GTCTC 2 cut(s) 53, 397
BsmBI CGTCTC 1 cut(s) 397
BsmFI GGGAC 2 cut(s) 547, 629
BsnI GGCC 3 cut(s) 399, 495, 832
BsoBI CYCGRG 1 cut(s) 506
Bsp1407I TGTACA 1 cut(s) 1123
Bsp143I GATC 2 cut(s) 4, 375
Bsp1720I GCTNAGC 1 cut(s) 987
BspACI CCGC 9 cut(s) 145, 154, 400, 500, 512, 517, 1076, 1097, 1100
BspANI GGCC 3 cut(s) 399, 495, 832
BspCNI CTCAG 1 cut(s) 979
BspFNI CGCG 2 cut(s) 514, 1078
BspHI TCATGA 1 cut(s) 862
BspLI GGNNCC 3 cut(s) 158, 496, 1095
BspPI GGATC 2 cut(s) 12, 370
BspT107I GGYRCC 1 cut(s) 156
BsrBI CCGCTC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 751
BsrGI TGTACA 1 cut(s) 1123
BsrI ACTGG 2 cut(s) 430, 481
BssECI CCNNGG 2 cut(s) 738, 1136
BssMI GATC 2 cut(s) 4, 375
BssNI GRCGYC 1 cut(s) 157
BssT1I CCWWGG 2 cut(s) 738, 1136
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 3 cut(s) 35, 474, 538
Bst6I CTCTTC 2 cut(s) 55, 137
BstACI GRCGYC 1 cut(s) 157
BstAPI GCANNNNNTGC 1 cut(s) 799
BstAUI TGTACA 1 cut(s) 1123
BstBAI YACGTR 2 cut(s) 556, 789
BstC8I GCNNGC 3 cut(s) 661, 812, 834
BstDEI CTNAG 2 cut(s) 621, 987
BstF5I GGATG 5 cut(s) 697, 732, 827, 907, 1146
BstFNI CGCG 2 cut(s) 514, 1078
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 2 cut(s) 159, 516
BstKTI GATC 2 cut(s) 7, 378
BstMAI GTCTC 2 cut(s) 53, 397
BstMBI GATC 2 cut(s) 4, 375
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 3 cut(s) 508, 511, 799
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 2 cut(s) 663, 814
BstSCI CCNGG 2 cut(s) 73, 1001
BstUI CGCG 2 cut(s) 514, 1078
BstV1I GCAGC 4 cut(s) 403, 489, 823, 826
BstZI CGGCCG 1 cut(s) 397
BsuRI GGCC 3 cut(s) 399, 495, 832
BtgZI GCGATG 1 cut(s) 458
BtsCI GGATG 5 cut(s) 697, 732, 827, 907, 1146
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 4 cut(s) 31, 340, 470, 488
Cac8I GCNNGC 3 cut(s) 661, 812, 834
CaiI CAGNNNCTG 1 cut(s) 839
CciI TCATGA 1 cut(s) 862
CfoI GCGC 2 cut(s) 159, 516
Cfr13I GGNCC 3 cut(s) 486, 494, 830
Csp6I GTAC 3 cut(s) 553, 709, 1124
CviAII CATG 8 cut(s) 150, 291, 645, 660, 799, 811, 863, 872
CviQI GTAC 3 cut(s) 553, 709, 1124
DdeI CTNAG 2 cut(s) 621, 987
DinI GGCGCC 1 cut(s) 158
DpnI GATC 2 cut(s) 6, 377
DpnII GATC 2 cut(s) 4, 375
EaeI YGGCCR 1 cut(s) 397
EagI CGGCCG 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 55, 137
EarI CTCTTC 2 cut(s) 55, 137
EclXI CGGCCG 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 738, 1136
Eco47I GGWCC 1 cut(s) 486
Eco52I CGGCCG 1 cut(s) 397
Eco72I CACGTG 1 cut(s) 789
Eco88I CYCGRG 1 cut(s) 506
EcoRI GAATTC 1 cut(s) 733
EcoRII CCWGG 1 cut(s) 73
EcoT14I CCWWGG 2 cut(s) 738, 1136
EgeI GGCGCC 1 cut(s) 158
EheI GGCGCC 1 cut(s) 158
ErhI CCWWGG 2 cut(s) 738, 1136
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 8 cut(s) 153, 294, 648, 663, 802, 814, 866, 875
FaqI GGGAC 2 cut(s) 547, 629
FatI CATG 8 cut(s) 149, 290, 644, 659, 798, 810, 862, 871
FauI CCCGC 1 cut(s) 507
FokI GGATG 5 cut(s) 704, 739, 834, 914, 1153
FspBI CTAG 3 cut(s) 272, 561, 1137
GlaI GCGC 2 cut(s) 158, 515
GsaI CCCAGC 1 cut(s) 952
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 3 cut(s) 399, 495, 832
HapII CCGG 2 cut(s) 396, 1002
HhaI GCGC 2 cut(s) 159, 516
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 8 cut(s) 153, 294, 648, 663, 802, 814, 866, 875
Hin6I GCGC 2 cut(s) 157, 514
HinP1I GCGC 2 cut(s) 157, 514
HinfI GANTC 1 cut(s) 965
HpaII CCGG 2 cut(s) 396, 1002
HphI GGTGA 3 cut(s) 221, 742, 970
Hpy166II GTNNAC 3 cut(s) 435, 553, 1126
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 4 cut(s) 8, 363, 532, 863
Hpy8I GTNNAC 3 cut(s) 435, 553, 1126
HpyCH4III ACNGT 3 cut(s) 35, 474, 538
HpyCH4IV ACGT 2 cut(s) 555, 788
HpyCH4V TGCA 5 cut(s) 95, 110, 749, 778, 842
HpyF10VI GCNNNNNNNGC 3 cut(s) 508, 511, 799
HpyF3I CTNAG 2 cut(s) 621, 987
HpySE526I ACGT 2 cut(s) 555, 788
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 8 cut(s) 153, 294, 648, 663, 802, 814, 866, 875
HspAI GCGC 2 cut(s) 157, 514
KasI GGCGCC 1 cut(s) 156
Kzo9I GATC 2 cut(s) 4, 375
LmnI GCTCC 1 cut(s) 1093
Lsp1109I GCAGC 4 cut(s) 403, 489, 823, 826
LweI GCATC 1 cut(s) 435
MaeI CTAG 3 cut(s) 272, 561, 1137
MaeII ACGT 2 cut(s) 555, 788
MalI GATC 2 cut(s) 6, 377
MbiI CCGCTC 1 cut(s) 147
MboI GATC 2 cut(s) 4, 375
MboII GAAGA 5 cut(s) 42, 124, 620, 630, 1050
MfeI CAATTG 1 cut(s) 702
MluCI AATT 4 cut(s) 247, 702, 733, 1080
Mly113I GGCGCC 1 cut(s) 157
MlyI GAGTC 1 cut(s) 974
MmeI TCCRAC 4 cut(s) 355, 490, 501, 1089
MseI TTAA 3 cut(s) 651, 666, 977
MslI CAYNNNNRTG 1 cut(s) 876
MspA1I CMGCKG 3 cut(s) 502, 519, 836
MspI CCGG 2 cut(s) 396, 1002
MspR9I CCNGG 2 cut(s) 75, 1003
MunI CAATTG 1 cut(s) 702
MvaI CCWGG 1 cut(s) 75
MvnI CGCG 2 cut(s) 514, 1078
MwoI GCNNNNNNNGC 3 cut(s) 508, 511, 799
NarI GGCGCC 1 cut(s) 157
NciI CCSGG 1 cut(s) 1003
NdeII GATC 2 cut(s) 4, 375
NlaIII CATG 8 cut(s) 153, 294, 648, 663, 802, 814, 866, 875
NlaIV GGNNCC 3 cut(s) 158, 496, 1095
NspI RCATGY 2 cut(s) 663, 814
PaeI GCATGC 2 cut(s) 663, 814
PagI TCATGA 1 cut(s) 862
PflMI CCANNNNNTGG 1 cut(s) 483
PleI GAGTC 1 cut(s) 973
PluTI GGCGCC 1 cut(s) 160
PmaCI CACGTG 1 cut(s) 789
PmlI CACGTG 1 cut(s) 789
PpsI GAGTC 1 cut(s) 973
Ppu21I YACGTR 2 cut(s) 556, 789
Psp6I CCWGG 1 cut(s) 73
PspCI CACGTG 1 cut(s) 789
PspFI CCCAGC 1 cut(s) 948
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 3 cut(s) 158, 496, 1095
PspPI GGNCC 3 cut(s) 486, 494, 830
PstNI CAGNNNCTG 1 cut(s) 839
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 3 cut(s) 554, 710, 1125
RsaNI GTAC 3 cut(s) 553, 709, 1124
RseI CAYNNNNRTG 1 cut(s) 876
SaqAI TTAA 3 cut(s) 651, 666, 977
Sau3AI GATC 2 cut(s) 4, 375
Sau96I GGNCC 3 cut(s) 486, 494, 830
SchI GAGTC 1 cut(s) 974
ScrFI CCNGG 2 cut(s) 75, 1003
SetI ASST 9 cut(s) 93, 102, 226, 558, 602, 680, 723, 791, 838
SfaNI GCATC 1 cut(s) 435
SfoI GGCGCC 1 cut(s) 158
SinI GGWCC 1 cut(s) 486
SmiMI CAYNNNNRTG 1 cut(s) 876
SmlI CTYRAG 2 cut(s) 42, 804
SmoI CTYRAG 2 cut(s) 42, 804
SphI GCATGC 2 cut(s) 663, 814
Sse9I AATT 4 cut(s) 247, 702, 733, 1080
SsiI CCGC 9 cut(s) 145, 154, 400, 500, 512, 517, 1076, 1097, 1100
SspDI GGCGCC 1 cut(s) 156
SspMI CTAG 3 cut(s) 272, 561, 1137
StyD4I CCNGG 2 cut(s) 73, 1001
StyI CCWWGG 2 cut(s) 738, 1136
TaaI ACNGT 3 cut(s) 35, 474, 538
TaiI ACGT 2 cut(s) 558, 791
TaqI TCGA 2 cut(s) 37, 602
TasI AATT 4 cut(s) 247, 702, 733, 1080
TatI WGTACW 1 cut(s) 1123
TauI GCSGC 7 cut(s) 147, 157, 402, 514, 519, 1099, 1102
Tru1I TTAA 3 cut(s) 651, 666, 977
Tru9I TTAA 3 cut(s) 651, 666, 977
TscAI CASTG 4 cut(s) 38, 347, 477, 488
TseI GCWGC 4 cut(s) 416, 502, 836, 839
TspDTI ATGAA 1 cut(s) 951
TspGWI ACGGA 3 cut(s) 115, 379, 1050
TspRI CASTG 4 cut(s) 38, 347, 477, 488
Van91I CCANNNNNTGG 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 486
XapI RAATTY 2 cut(s) 733, 1080
XceI RCATGY 2 cut(s) 663, 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 1080
XmaJI CCTAGG 1 cut(s) 1136
XspI CTAG 3 cut(s) 272, 561, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.