Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005313

Physical Location & Seq

Genomic Coordinates Forward (+)

3633090 .. 3641231

Gene Structure 8142 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1030500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGGGTTGCTTACACTCCAAAACCGCCCACCTCCTCCGCTCCCCCGACCACCCCTCTTCTCTACCCGACACCCACAAACCTGACCCGGCTAATGGGGATCCAGCTGCGGCGGCGGATCCCCTGGTGCCGGCATTCAAAGAGTACGGCGTCGACGAGCTCCGGAAGGCCACGAACGGGTTCAGCTCCGAATGCATTGTTTCCGAAAGCGGCGAAAAAGCTCCCAATGTTGTTTACAGAGGGAAGCTTGATGGCAATCGCCTTGTCGCTGTTAAACGCTTCTCCAAGCAATCTTGGCCCGACGCCCAGCAGTTCGTGGCTGAGGCTTCTGGAGTAGGGAAGTTGAGGCACAAGAGATTGGTGAATCTGATTGGTTGCTCTGCCGAGGGTGACGAACGGCTCTTGGTGGCCGAGTATATGCCTAATGATACTCTTTCCAAGCATCTCTTCCATTGGGACAAGAAGCCATTGCCATGGGAAATGCGTGTTAGAGTTGCATACCATATTGCAGAGGCACTTGATCATTGTAATACAGAAAACCGGAAGATCTATCATGATTTGAATGCATATAGAGTTCTTTTTGACGAGGATGGTGACCCTCGATTGTCTACCTTTGGCCTTATGAAAAATAGTCGGGATGGAAAAAGCTACAGTACCAATTTGGCTTATACTCCACCTGAGTTTCTACGGACAGGCAGGGTCATCCCAGAGAGTGTAATCTACAGTTATGGAACTGTCCTGTTGGACCTTTTGAGTGGAAAGCATATTCCTCCAAGCCATGCACTGGATTTGATAAGGGGAAAGAATGTATTGCTGTTGATGGATTCATCATTGGAAGGGCAGTATGCGAATGATGATGCCACGAAGTTGGTTGAACTTGCTTCCAAATGTCTTCAGTCTGAGGCCAGGGATAGAGGAGATATTAAGTTTCTTCTCACAGCCATTGCCCCGTTTCAAAAACAGAAAGAGGTGGCATCTCATGTTTTAATGGGCCTCCCAAAAAACACTGTTGTGGTGCCAACCATGCTTTCTCCCCTTGGAAAGGCGTGTGCCAGGATGGATCTTACTGCTGTGCATGATATTTTGCTAAAAACAGGCTATAAAGATGAGGAAGGTGCCGAAAATGAGCTCTCATTCCAAGAATGGACACAACAAGTCCAAGATATGTTGAACACAAAGAAATTTGGTGATATTGCATTTAGGGATAAGGATTTCAAGAATGCAATCGAGTATTATTCCAAGTTGGTTGCTATGATATCTGGCCCTCCAGCAACTGTCTTTGCGAGAAGAGCCTTCTCGTACTTAATGAATGGCCAAGCAGAGCTTGCTTTAAGAGATGCGATGCAGGCACAGGTATGCATACCAGAGTGGCCGACTGCATTCTACTTGCAGGCGCTTGCGCTCTCAAGGCTCGGAATGGAGACTGATGCTCAGGACATGCTAAATGATGGAGCGGCCTTTGACACAAAGAAACACAACGCCTGGCGTAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048545 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

497

Amino Acids

55.31

Weight (kDa)

6.45

Isoelectric Point (pI)

34.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	76 - 311	1.2e-25	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	76 - 306	5.6e-25	Protein kinase domain
TPR_BSK1_C	PF25575	347 - 464	9.4e-54	Serine/threonine-protein kinase BSK1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46570
fragaria_vesca	FvH4_5g21570 FvH4_5g21570 FvH4_5g21570 FvH4_5g21570 FvH4_5g21570
malus_domestica	MD06G1030500.v1.1 MD07G1082900.v1.1
prunus_persica	Prupe.2G110200_v2.0.a1 Prupe.5G041700_v2.0.a1 Prupe.5G041700_v2.0.a1 Prupe.5G041700_v2.0.a1 Prupe.5G041700_v2.0.a1 Prupe.5G041700_v2.0.a1
pyrus_communis	pycom06g02520 pycom07g06620 pycom16g24750
rosa_chinensis	RchiOBHm_Chr7g0208991
rosa_laevigata	RLG00000003174
rosa_multiflora	Rmu_sc0002829.1_g000001
rosa_roxburghii	Rroxscaffold_3G00249910 Rroxscaffold_5G00335930
rosa_rugosa	Rorug07G0107900 Rorug07G0108000
rosa_samantha	Rh7AG242700 Rh7BG237500 Rh7CG257800 Rh7DG249600
rosa_wichuraiana	Rw7G020560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	124, 1012, 1112
AccB7I	CCANNNNNTGG	1 cut(s)	781
AccBSI	CCGCTC	2 cut(s)	39, 1451
AccI	GTMKAC	2 cut(s)	150, 605
AccIII	TCCGGA	1 cut(s)	159
AciI	CCGC	7 cut(s)	24, 37, 107, 110, 113, 207, 1451
AclWI	GGATC	5 cut(s)	92, 105, 110, 123, 1065
AcoI	YGGCCR	3 cut(s)	405, 1309, 1367
AcsI	RAATTY	1 cut(s)	1178
AcuI	CTGAAG	1 cut(s)	875
AcyI	GRCGYC	2 cut(s)	147, 300
AfaI	GTAC	3 cut(s)	143, 652, 1298
AfiI	CCNNNNNNNGG	5 cut(s)	92, 127, 174, 781, 1039
AgsI	TTSAA	6 cut(s)	136, 559, 872, 953, 1168, 1213
AjnI	CCWGG	4 cut(s)	120, 902, 1049, 1478
AjuI	GAANNNNNNNTTGG	4 cut(s)	428, 460, 1009, 1041
AleI	CACNNNNGTG	1 cut(s)	1007
AluBI	AGCT	8 cut(s)	104, 157, 183, 218, 244, 645, 1126, 1321
AluI	AGCT	8 cut(s)	104, 157, 183, 218, 244, 645, 1126, 1321
Alw21I	GWGCWC	2 cut(s)	159, 1128
Alw26I	GTCTC	1 cut(s)	1412
AlwI	GGATC	5 cut(s)	92, 105, 110, 123, 1065
AlwNI	CAGNNNCTG	1 cut(s)	1271
Aor13HI	TCCGGA	1 cut(s)	159
ApeKI	GCWGC	1 cut(s)	104
ApoI	RAATTY	1 cut(s)	1178
Asp700I	GAANNNNTTC	1 cut(s)	176
AspLEI	GCGC	2 cut(s)	1393, 1399
AspS9I	GGNCC	4 cut(s)	294, 742, 988, 1259
AsuC2I	CCSGG	1 cut(s)	86
AsuHPI	GGTGA	4 cut(s)	370, 398, 602, 1196
AvaII	GGWCC	1 cut(s)	742
BalI	TGGCCA	1 cut(s)	1311
BamHI	GGATCC	2 cut(s)	97, 115
BanI	GGYRCC	3 cut(s)	124, 1012, 1112
BanII	GRGCYC	2 cut(s)	159, 1128
BbsI	GAAGAC	1 cut(s)	881
Bbv12I	GWGCWC	2 cut(s)	159, 1128
BbvCI	CCTCAGC	1 cut(s)	318
BbvI	GCAGC	1 cut(s)	91
BccI	CCATC	6 cut(s)	242, 581, 629, 811, 1048, 1439
BceAI	ACGGC	2 cut(s)	160, 410
BciT130I	CCWGG	4 cut(s)	122, 904, 1051, 1480
BclI	TGATCA	1 cut(s)	517
BcnI	CCSGG	1 cut(s)	86
BcoDI	GTCTC	1 cut(s)	1412
BfmI	CTRYAG	2 cut(s)	646, 718
BfoI	RGCGCY	1 cut(s)	1394
BglII	AGATCT	1 cut(s)	543
BisI	GCNGC	5 cut(s)	105, 108, 111, 208, 1452
BlsI	GCNGC	5 cut(s)	106, 109, 112, 209, 1453
Bme1390I	CCNGG	5 cut(s)	86, 122, 904, 1051, 1480
Bme18I	GGWCC	1 cut(s)	742
BmgT120I	GGNCC	4 cut(s)	294, 742, 988, 1259
BmiI	GGNNCC	5 cut(s)	99, 117, 126, 1014, 1114
BmrFI	CCNGG	5 cut(s)	86, 122, 904, 1051, 1480
BmsI	GCATC	6 cut(s)	448, 844, 980, 1324, 1329, 1414
BpiI	GAAGAC	1 cut(s)	881
BpmI	CTGGAG	2 cut(s)	348, 1248
Bpu10I	CCTNAGC	2 cut(s)	318, 1428
BpuEI	CTTGAG	1 cut(s)	1387
BpuMI	CCSGG	1 cut(s)	86
BsaBI	GATNNNNATC	1 cut(s)	252
BsaHI	GRCGYC	2 cut(s)	147, 300
BsaJI	CCNNGG	5 cut(s)	120, 381, 470, 903, 1033
BsaWI	WCCGGW	2 cut(s)	159, 537
Bsc4I	CCNNNNNNNGG	5 cut(s)	92, 127, 174, 781, 1039
Bse118I	RCCGGY	1 cut(s)	127
Bse1I	ACTGG	1 cut(s)	786
Bse3DI	GCAATG	2 cut(s)	464, 939
Bse8I	GATNNNNATC	1 cut(s)	252
BseAI	TCCGGA	1 cut(s)	159
BseBI	CCWGG	4 cut(s)	122, 904, 1051, 1480
BseDI	CCNNGG	5 cut(s)	120, 381, 470, 903, 1033
BseGI	GGATG	4 cut(s)	592, 640, 699, 1059
BseJI	GATNNNNATC	1 cut(s)	252
BseLI	CCNNNNNNNGG	5 cut(s)	92, 127, 174, 781, 1039
BseMI	GCAATG	2 cut(s)	464, 939
BseMII	CTCAG	4 cut(s)	309, 666, 888, 1442
BseNI	ACTGG	1 cut(s)	786
BseRI	GAGGAG	2 cut(s)	23, 927
BseXI	GCAGC	1 cut(s)	91
BseYI	CCCAGC	1 cut(s)	303
BshNI	GGYRCC	3 cut(s)	124, 1012, 1112
BsiHKAI	GWGCWC	2 cut(s)	159, 1128
BsiSI	CCGG	4 cut(s)	86, 128, 160, 538
BslFI	GGGAC	1 cut(s)	467
BslI	CCNNNNNNNGG	5 cut(s)	92, 127, 174, 781, 1039
BsmAI	GTCTC	1 cut(s)	1412
BsmFI	GGGAC	1 cut(s)	467
BsmI	GAATGC	5 cut(s)	131, 194, 565, 1222, 1376
Bsp1286I	GDGCHC	2 cut(s)	159, 1128
Bsp13I	TCCGGA	1 cut(s)	159
Bsp143I	GATC	5 cut(s)	97, 115, 517, 543, 1057
Bsp19I	CCATGG	1 cut(s)	470
BspACI	CCGC	7 cut(s)	24, 37, 107, 110, 113, 207, 1451
BspCNI	CTCAG	4 cut(s)	310, 667, 889, 1441
BspEI	TCCGGA	1 cut(s)	159
BspHI	TCATGA	1 cut(s)	550
BspLI	GGNNCC	5 cut(s)	99, 117, 126, 1014, 1114
BspPI	GGATC	5 cut(s)	92, 105, 110, 123, 1065
BspQI	GCTCTTC	1 cut(s)	1279
BspT107I	GGYRCC	3 cut(s)	124, 1012, 1112
BsrBI	CCGCTC	2 cut(s)	39, 1451
BsrDI	GCAATG	2 cut(s)	464, 939
BsrFI	RCCGGY	1 cut(s)	127
BsrI	ACTGG	1 cut(s)	786
BssAI	RCCGGY	1 cut(s)	127
BssECI	CCNNGG	5 cut(s)	120, 381, 470, 903, 1033
BssMI	GATC	5 cut(s)	97, 115, 517, 543, 1057
BssNI	GRCGYC	2 cut(s)	147, 300
BssT1I	CCWWGG	2 cut(s)	470, 1033
Bst2UI	CCWGG	4 cut(s)	122, 904, 1051, 1480
Bst4CI	ACNGT	5 cut(s)	650, 722, 733, 1006, 1273
Bst6I	CTCTTC	3 cut(s)	61, 449, 1279
BstACI	GRCGYC	2 cut(s)	147, 300
BstAPI	GCANNNNNTGC	1 cut(s)	1322
BstC8I	GCNNGC	5 cut(s)	129, 1323, 1344, 1389, 1395
BstDEI	CTNAG	4 cut(s)	318, 675, 897, 1428
BstDSI	CCRYGG	1 cut(s)	470
BstEII	GGTNACC	1 cut(s)	590
BstENI	CCTNNNNNAGG	1 cut(s)	1037
BstF5I	GGATG	4 cut(s)	592, 640, 699, 1059
BstH2I	RGCGCY	1 cut(s)	1394
BstHHI	GCGC	2 cut(s)	1393, 1399
BstKTI	GATC	5 cut(s)	100, 118, 520, 546, 1060
BstMAI	GTCTC	1 cut(s)	1412
BstMBI	GATC	5 cut(s)	97, 115, 517, 543, 1057
BstMWI	GCNNNNNNNGC	8 cut(s)	110, 189, 292, 1021, 1286, 1322, 1343, 1405
BstNI	CCWGG	4 cut(s)	122, 904, 1051, 1480
BstNSI	RCATGY	1 cut(s)	1438
BstPI	GGTNACC	1 cut(s)	590
BstSCI	CCNGG	5 cut(s)	84, 120, 902, 1049, 1478
BstSFI	CTRYAG	2 cut(s)	646, 718
BstV1I	GCAGC	1 cut(s)	91
BstV2I	GAAGAC	1 cut(s)	881
BstX2I	RGATCY	4 cut(s)	97, 115, 543, 1057
BstXI	CCANNNNNNTGG	2 cut(s)	471, 865
BstYI	RGATCY	4 cut(s)	97, 115, 543, 1057
BtgI	CCRYGG	1 cut(s)	470
BtgZI	GCGATG	1 cut(s)	1352
BtsCI	GGATG	4 cut(s)	592, 640, 699, 1059
BtsIMutI	CAGTG	2 cut(s)	779, 1002
Cac8I	GCNNGC	5 cut(s)	129, 1323, 1344, 1389, 1395
CaiI	CAGNNNCTG	1 cut(s)	1271
CciI	TCATGA	1 cut(s)	550
CfoI	GCGC	2 cut(s)	1393, 1399
Cfr10I	RCCGGY	1 cut(s)	127
Cfr13I	GGNCC	4 cut(s)	294, 742, 988, 1259
CseI	GACGC	2 cut(s)	136, 308
Csp6I	GTAC	3 cut(s)	142, 651, 1297
CviAII	CATG	7 cut(s)	471, 551, 776, 977, 1021, 1073, 1435
CviQI	GTAC	3 cut(s)	142, 651, 1297
DdeI	CTNAG	4 cut(s)	318, 675, 897, 1428
DpnI	GATC	5 cut(s)	99, 117, 519, 545, 1059
DpnII	GATC	5 cut(s)	97, 115, 517, 543, 1057
EaeI	YGGCCR	3 cut(s)	405, 1309, 1367
Eam1104I	CTCTTC	3 cut(s)	61, 449, 1279
EarI	CTCTTC	3 cut(s)	61, 449, 1279
EciI	GGCGGA	1 cut(s)	128
Ecl136II	GAGCTC	2 cut(s)	157, 1126
Eco130I	CCWWGG	2 cut(s)	470, 1033
Eco24I	GRGCYC	2 cut(s)	159, 1128
Eco32I	GATATC	1 cut(s)	1254
Eco47I	GGWCC	1 cut(s)	742
Eco53kI	GAGCTC	2 cut(s)	157, 1126
Eco57I	CTGAAG	1 cut(s)	875
Eco91I	GGTNACC	1 cut(s)	590
EcoICRI	GAGCTC	2 cut(s)	157, 1126
EcoNI	CCTNNNNNAGG	1 cut(s)	1037
EcoO65I	GGTNACC	1 cut(s)	590
EcoRII	CCWGG	4 cut(s)	120, 902, 1049, 1478
EcoRV	GATATC	1 cut(s)	1254
EcoT14I	CCWWGG	2 cut(s)	470, 1033
EcoT22I	ATGCAT	3 cut(s)	194, 565, 1358
EcoT38I	GRGCYC	2 cut(s)	159, 1128
ErhI	CCWWGG	2 cut(s)	470, 1033
FaeI	CATG	7 cut(s)	474, 554, 779, 980, 1024, 1076, 1438
FalI	AAGNNNNNCTT	4 cut(s)	428, 460, 1305, 1337
FaqI	GGGAC	1 cut(s)	467
FatI	CATG	7 cut(s)	470, 550, 775, 976, 1020, 1072, 1434
FbaI	TGATCA	1 cut(s)	517
FblI	GTMKAC	2 cut(s)	150, 605
Fnu4HI	GCNGC	5 cut(s)	105, 108, 111, 208, 1452
FokI	GGATG	4 cut(s)	599, 647, 686, 1066
FriOI	GRGCYC	2 cut(s)	159, 1128
Fsp4HI	GCNGC	5 cut(s)	105, 108, 111, 208, 1452
GlaI	GCGC	2 cut(s)	1392, 1398
GluI	GCNGC	5 cut(s)	105, 108, 111, 208, 1452
GsaI	CCCAGC	1 cut(s)	307
GsuI	CTGGAG	2 cut(s)	348, 1248
HaeII	RGCGCY	1 cut(s)	1394
HapII	CCGG	4 cut(s)	86, 128, 160, 538
HgaI	GACGC	2 cut(s)	136, 308
HhaI	GCGC	2 cut(s)	1393, 1399
Hin1I	GRCGYC	2 cut(s)	147, 300
Hin1II	CATG	7 cut(s)	474, 554, 779, 980, 1024, 1076, 1438
Hin6I	GCGC	2 cut(s)	1391, 1397
HinP1I	GCGC	2 cut(s)	1391, 1397
HincII	GTYRAC	1 cut(s)	151
HindII	GTYRAC	1 cut(s)	151
HindIII	AAGCTT	1 cut(s)	242
HinfI	GANTC	2 cut(s)	361, 821
HpaII	CCGG	4 cut(s)	86, 128, 160, 538
HphI	GGTGA	4 cut(s)	370, 398, 602, 1196
Hpy166II	GTNNAC	3 cut(s)	151, 232, 606
Hpy188I	TCNGA	5 cut(s)	187, 202, 366, 898, 1412
Hpy188III	TCNNGA	6 cut(s)	160, 327, 551, 632, 1213, 1430
Hpy8I	GTNNAC	3 cut(s)	151, 232, 606
Hpy99I	CGWCG	3 cut(s)	152, 155, 302
HpyAV	CCTTC	4 cut(s)	157, 827, 1103, 1300
HpyCH4III	ACNGT	5 cut(s)	650, 722, 733, 1006, 1273
HpyF10VI	GCNNNNNNNGC	8 cut(s)	110, 189, 292, 1021, 1286, 1322, 1343, 1405
HpyF3I	CTNAG	4 cut(s)	318, 675, 897, 1428
Hsp92I	GRCGYC	2 cut(s)	147, 300
Hsp92II	CATG	7 cut(s)	474, 554, 779, 980, 1024, 1076, 1438
HspAI	GCGC	2 cut(s)	1391, 1397
Kpn2I	TCCGGA	1 cut(s)	159
KroI	GCCGGC	1 cut(s)	127
KroNI	GCCGGC	1 cut(s)	129
Ksp22I	TGATCA	1 cut(s)	517
Kzo9I	GATC	5 cut(s)	97, 115, 517, 543, 1057
LguI	GCTCTTC	1 cut(s)	1279
LmnI	GCTCC	5 cut(s)	44, 162, 188, 223, 1448
Lsp1109I	GCAGC	1 cut(s)	91
LweI	GCATC	6 cut(s)	448, 844, 980, 1324, 1329, 1414
MaeIII	GTNAC	3 cut(s)	386, 590, 1484
MalI	GATC	5 cut(s)	99, 117, 519, 545, 1059
MbiI	CCGCTC	2 cut(s)	39, 1451
MboI	GATC	5 cut(s)	97, 115, 517, 543, 1057
MboII	GAAGA	6 cut(s)	48, 436, 553, 881, 920, 1296
MflI	RGATCY	4 cut(s)	97, 115, 543, 1057
MhlI	GDGCHC	2 cut(s)	159, 1128
MlsI	TGGCCA	1 cut(s)	1311
MluCI	AATT	2 cut(s)	655, 1178
MluNI	TGGCCA	1 cut(s)	1311
MmeI	TCCRAC	1 cut(s)	720
Mox20I	TGGCCA	1 cut(s)	1311
Mph1103I	ATGCAT	3 cut(s)	194, 565, 1358
MroI	TCCGGA	1 cut(s)	159
MroNI	GCCGGC	1 cut(s)	127
MroXI	GAANNNNTTC	1 cut(s)	176
MscI	TGGCCA	1 cut(s)	1311
MseI	TTAA	5 cut(s)	270, 921, 983, 1301, 1328
MslI	CAYNNNNRTG	3 cut(s)	469, 1007, 1351
Msp20I	TGGCCA	1 cut(s)	1311
MspA1I	CMGCKG	1 cut(s)	104
MspI	CCGG	4 cut(s)	86, 128, 160, 538
MspR9I	CCNGG	5 cut(s)	86, 122, 904, 1051, 1480
Mva1269I	GAATGC	5 cut(s)	131, 194, 565, 1222, 1376
MvaI	CCWGG	4 cut(s)	122, 904, 1051, 1480
MwoI	GCNNNNNNNGC	8 cut(s)	110, 189, 292, 1021, 1286, 1322, 1343, 1405
NaeI	GCCGGC	1 cut(s)	129
NciI	CCSGG	1 cut(s)	86
NcoI	CCATGG	1 cut(s)	470
NdeII	GATC	5 cut(s)	97, 115, 517, 543, 1057
NgoMIV	GCCGGC	1 cut(s)	127
NlaIII	CATG	7 cut(s)	474, 554, 779, 980, 1024, 1076, 1438
NlaIV	GGNNCC	5 cut(s)	99, 117, 126, 1014, 1114
NmeAIII	GCCGAG	2 cut(s)	406, 433
NmuCI	GTSAC	2 cut(s)	386, 590
NsiI	ATGCAT	3 cut(s)	194, 565, 1358
NspI	RCATGY	1 cut(s)	1438
OliI	CACNNNNGTG	1 cut(s)	1007
PagI	TCATGA	1 cut(s)	550
PciSI	GCTCTTC	1 cut(s)	1279
PcsI	WCGNNNNNNNCGW	1 cut(s)	150
PctI	GAATGC	5 cut(s)	131, 194, 565, 1222, 1376
PdiI	GCCGGC	1 cut(s)	129
PdmI	GAANNNNTTC	1 cut(s)	176
PfeI	GAWTC	2 cut(s)	361, 821
PflMI	CCANNNNNTGG	1 cut(s)	781
PkrI	GCNGC	5 cut(s)	106, 109, 112, 209, 1453
Psp124BI	GAGCTC	2 cut(s)	159, 1128
Psp6I	CCWGG	4 cut(s)	120, 902, 1049, 1478
PspEI	GGTNACC	1 cut(s)	590
PspFI	CCCAGC	1 cut(s)	303
PspGI	CCWGG	4 cut(s)	120, 902, 1049, 1478
PspN4I	GGNNCC	5 cut(s)	99, 117, 126, 1014, 1114
PspPI	GGNCC	4 cut(s)	294, 742, 988, 1259
PstNI	CAGNNNCTG	1 cut(s)	1271
PsuI	RGATCY	4 cut(s)	97, 115, 543, 1057
PvuII	CAGCTG	1 cut(s)	104
RsaI	GTAC	3 cut(s)	143, 652, 1298
RsaNI	GTAC	3 cut(s)	142, 651, 1297
RseI	CAYNNNNRTG	3 cut(s)	469, 1007, 1351
SacI	GAGCTC	2 cut(s)	159, 1128
SalI	GTCGAC	1 cut(s)	149
SapI	GCTCTTC	1 cut(s)	1279
SaqAI	TTAA	5 cut(s)	270, 921, 983, 1301, 1328
SatI	GCNGC	5 cut(s)	105, 108, 111, 208, 1452
Sau3AI	GATC	5 cut(s)	97, 115, 517, 543, 1057
Sau96I	GGNCC	4 cut(s)	294, 742, 988, 1259
ScrFI	CCNGG	5 cut(s)	86, 122, 904, 1051, 1480
SduI	GDGCHC	2 cut(s)	159, 1128
SfaNI	GCATC	6 cut(s)	448, 844, 980, 1324, 1329, 1414
SfcI	CTRYAG	2 cut(s)	646, 718
SgrDI	CGTCGACG	1 cut(s)	149
SinI	GGWCC	1 cut(s)	742
SmiMI	CAYNNNNRTG	3 cut(s)	469, 1007, 1351
SmlI	CTYRAG	1 cut(s)	1402
SmoI	CTYRAG	1 cut(s)	1402
Sse9I	AATT	2 cut(s)	655, 1178
SsiI	CCGC	7 cut(s)	24, 37, 107, 110, 113, 207, 1451
SstI	GAGCTC	2 cut(s)	159, 1128
StyD4I	CCNGG	5 cut(s)	84, 120, 902, 1049, 1478
StyI	CCWWGG	2 cut(s)	470, 1033
TaaI	ACNGT	5 cut(s)	650, 722, 733, 1006, 1273
TaqI	TCGA	3 cut(s)	150, 598, 1224
TasI	AATT	2 cut(s)	655, 1178
TauI	GCSGC	4 cut(s)	110, 113, 210, 1454
TfiI	GAWTC	2 cut(s)	361, 821
Tru1I	TTAA	5 cut(s)	270, 921, 983, 1301, 1328
Tru9I	TTAA	5 cut(s)	270, 921, 983, 1301, 1328
TscAI	CASTG	2 cut(s)	786, 1009
TseFI	GTSAC	2 cut(s)	386, 590
TseI	GCWGC	1 cut(s)	104
Tsp45I	GTSAC	2 cut(s)	386, 590
TspDTI	ATGAA	3 cut(s)	635, 813, 1319
TspGWI	ACGGA	1 cut(s)	700
TspRI	CASTG	2 cut(s)	786, 1009
Van91I	CCANNNNNTGG	1 cut(s)	781
VpaK11BI	GGWCC	1 cut(s)	742
XagI	CCTNNNNNAGG	1 cut(s)	1037
XapI	RAATTY	1 cut(s)	1178
XceI	RCATGY	1 cut(s)	1438
XmiI	GTMKAC	2 cut(s)	150, 605
XmnI	GAANNNNTTC	1 cut(s)	176
Zsp2I	ATGCAT	3 cut(s)	194, 565, 1358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.