Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006782

Physical Location & Seq

Genomic Coordinates Forward (+)

20717336 .. 20721090

Gene Structure 3755 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1084400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1905 bp

ATGGCGGTGTCTGAGAACCTGAGAAAACAGCTTGCTCTTTCTGTGAGAAGTATCCAGTGGAGCTATGGAATTTTCTGGTCCATTTCAGCCAGACAACCAGGGGTGTTAGAGTGGGGCGATGGGTACTACAATGGAGATATAAAGACCAGAAAAACAGTTCAAGCCGTAGAGCTTGATGCTGACCAAATGGGTTTGCAAAGGAGTGAGCAACTGAGAGAACTTTATGATTCCCTCTCAGCGGGCGAAGCAAGTCCGCAGGCTAGAAGGCCTTCTGCATCATTATCACCCGAAGATCTGGCTGATACTGAGTGGTATTACCTAGTTTGTATGTCGTTCGTCTTCAACATTGGCCAAGGGTTGCCAGGAAGAACATTAGCAAATGGCAAACCTACCTGGCTATGCAATGCTCACTACGTGGATAGTAAAGTGTTTTCTCGTTCACTACTAGCAAAGAGCGCATCCATTCAGACTGTTGTATGCTTTCCATTTTTGGGAGGAGTGATCGAGCTCGGTGTGACTGAACTGGTTCTAGAGGACCCTAGTCTCATTCAGCATGTTAAAACATCTTTCATGGAGGCTCCATATCCCATAATCGCTTCCAAGAGAACCAATCCTAGTGCAGGAAGCACAAGAAACGACAATGATCTTGCTTGTGCCATGCTTGATGATGGTATAGTCAACACCAAATTAATTCCTGTTGTAGAGTGTCAAGAAATGGATGTGACTTCACCTGATGACAATTCAAATGGTTTGGGTCCTAATCAACCGGCTGATGATTCATTCATGGTGGAAGGGATGAACGGAGGAGCTTCCCAAGTGCAAAGCTGGCAATTTATGGACGATGAGTTAAGTAATTTTGTGCACCATTCCATGGATTCCAGTGATTGTATATCTCAGACTTTGGTGTATCCGGAAAAGGTTCTCTCTGGTCCTAAGACTGAACAGGCAAATGATCACTGCCCGCATGAACATAAAGAGTGCAATAGCACTAAAAAGACCTCTTTGGATCCTCAAGGCAATGACTTGCAGTATAAAAGTGTTCTTTCTGCTCTTTTGAAGAGCTCACACCAATTGGTTTTGGGGCCACACTTCAAGAATTCTCATCAGGAATCCAGCTTTACCAGTTGGAAGAGAGGAGGGTTTGTAAAATGCCTGACACAAAGAGGTGGAACCCCGCAAAAATTATTGAAGAAGATTTTGCTTGAAGTTCCTCAGATGCATGTTGACTGTGTGCTTGAGCCCCCAGAAGATACCAGCAATGTAAATGGAGTTTGTAGACCAGAGGCTGATGATATTGATACAAATCATGCATTATCTGATGAGAGGAGGCGGAGGGAAAGACTGAATGAAAGATTTTGCATTTTGAAATCAATGGTTCCCTCGGTTAGCAAGGAGGACGAGGTATCCATTTTAGATGATGCAATAGAGCATTTGAAAGATCTTGAGAAAAGGGTTGAAGAGTTGGAATCGTTTTGGGAGCCAACAAATTCATACTCAAAAATGAAGAGGAAACTCCAAGATACAGTTGAGAGAACATCTGACAACTGTTGCAACCCCAAAATTAGCAATGGGAAGAAGCCTATAGTTTACAAGAGGAAGGTCAGCAACGTTGATGAAACAGAACCAGAAATCAATCACATTGTATCGAAGAACATTTCAAGTGATAATATAACAGTTAATATGAACAACAAAGATGTTCTAATAGAGATGAAGTTTCCTTGGAGGGAGGGAGTGTTGCTAGAGATCATGGATGCCACAAGCCGGCTTCATTTAGATGCTCACTCGGTTCAATCATCCACCGCGGATGGAATTCTTTCCCTGACCATTAAATCCAGGTTCAAGGGGACGAGTATTGCATCAGCAGGGACAATCAAGCAAGCACTTCATAGAATTGCCAGAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0001101 GO:0001708 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0008544 GO:0009653 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009913 GO:0009957 GO:0009962 GO:0009963 GO:0009987 GO:0010026 GO:0010033 GO:0010090 GO:0010091 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0023052 GO:0030154 GO:0030855 GO:0031323 GO:0031326 GO:0031537 GO:0031539 GO:0031540 GO:0031542 GO:0032502 GO:0032870 GO:0032989 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045165 GO:0048468 GO:0048518 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0060429 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071840 GO:0080090 GO:0090558 GO:0090626 GO:0140110 GO:1901700 GO:1901701 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

635

Amino Acids

70.66

Weight (kDa)

5.6

Isoelectric Point (pI)

47.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	7 - 190	7.5e-53	bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH_SAC51	PF23173	433 - 484	1.5e-06	Transcription factor SAC51 bHLH domain
HLH	PF00010	440 - 482	1.7e-09	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	556 - 632	1e-21	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63650 AT1G63650 AT1G63650 AT5G41315 AT5G41315 AT5G41315
fragaria_vesca	FvH4_5g02520
malus_domestica	MD06G1084400.v1.1 MD14G1105500.v1.1
prunus_persica	Prupe.5G100700_v2.0.a1 Prupe.5G100700_v2.0.a1 Prupe.5G100700_v2.0.a1
pyrus_communis	pycom06g08070 pycom14g10100
rosa_chinensis	RchiOBHm_Chr7g0197531
rosa_laevigata	RLG00000003927
rosa_multiflora	Rmu_sc0007007.1_g000003
rosa_roxburghii	Rroxscaffold_3G00258370
rosa_rugosa	Rorug07G0040800
rosa_samantha	Rh1AG185600 Rh1CG171400 Rh7AG165700 Rh7BG167700 Rh7CG174100 Rh7DG167100
rosa_wichuraiana	Rw7G014420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	871
AccI	GTMKAC	1 cut(s)	1276
AccII	CGCG	1 cut(s)	1802
AccIII	TCCGGA	1 cut(s)	910
AciI	CCGC	8 cut(s)	5, 239, 254, 962, 1175, 1330, 1800, 1802
AclI	AACGTT	1 cut(s)	1608
AclWI	GGATC	2 cut(s)	1001, 1014
AcoI	YGGCCR	1 cut(s)	349
AcsI	RAATTY	4 cut(s)	69, 1096, 1486, 1809
AdeI	CACNNNGTG	1 cut(s)	415
AfaI	GTAC	1 cut(s)	125
AfiI	CCNNNNNNNGG	5 cut(s)	238, 491, 620, 871, 1761
AjnI	CCWGG	4 cut(s)	97, 361, 392, 1832
AluBI	AGCT	9 cut(s)	31, 63, 172, 508, 809, 825, 1062, 1116, 1902
AluI	AGCT	9 cut(s)	31, 63, 172, 508, 809, 825, 1062, 1116, 1902
Alw21I	GWGCWC	3 cut(s)	510, 864, 1064
Alw26I	GTCTC	1 cut(s)	548
Alw44I	GTGCAC	1 cut(s)	860
AlwI	GGATC	2 cut(s)	1001, 1014
AlwNI	CAGNNNCTG	2 cut(s)	1286, 1902
Aor13HI	TCCGGA	1 cut(s)	910
AoxI	GGCC	3 cut(s)	266, 349, 1082
ApaLI	GTGCAC	1 cut(s)	860
ApoI	RAATTY	4 cut(s)	69, 1096, 1486, 1809
AseI	ATTAAT	1 cut(s)	689
Asp700I	GAANNNNTTC	4 cut(s)	268, 525, 918, 1813
AspLEI	GCGC	1 cut(s)	458
AspS9I	GGNCC	5 cut(s)	78, 535, 755, 929, 1082
AsuHPI	GGTGA	2 cut(s)	276, 720
AvaII	GGWCC	4 cut(s)	78, 535, 755, 929
BaeGI	GKGCMC	1 cut(s)	864
BalI	TGGCCA	1 cut(s)	351
BamHI	GGATCC	1 cut(s)	1006
BanII	GRGCYC	3 cut(s)	510, 1064, 1242
BbsI	GAAGAC	1 cut(s)	331
Bbv12I	GWGCWC	3 cut(s)	510, 864, 1064
BccI	CCATC	3 cut(s)	113, 662, 1799
BceAI	ACGGC	1 cut(s)	149
BciT130I	CCWGG	4 cut(s)	99, 363, 394, 1834
BciVI	GTATCC	3 cut(s)	62, 918, 1414
BclI	TGATCA	1 cut(s)	952
BcoDI	GTCTC	1 cut(s)	548
BfaI	CTAG	7 cut(s)	261, 320, 446, 530, 540, 615, 1739
BfmI	CTRYAG	1 cut(s)	1581
BfuI	GTATCC	3 cut(s)	62, 918, 1414
BglII	AGATCT	2 cut(s)	292, 1438
Bme1390I	CCNGG	4 cut(s)	99, 363, 394, 1834
Bme18I	GGWCC	4 cut(s)	78, 535, 755, 929
BmgT120I	GGNCC	5 cut(s)	78, 535, 755, 929, 1082
BmiI	GGNNCC	8 cut(s)	537, 579, 756, 1008, 1083, 1171, 1377, 1479
BmrFI	CCNGG	4 cut(s)	99, 363, 394, 1834
BmsI	GCATC	8 cut(s)	166, 284, 467, 1206, 1408, 1741, 1765, 1865
BoxI	GACNNNNGTC	1 cut(s)	540
BpiI	GAAGAC	1 cut(s)	331
BpuEI	CTTGAG	3 cut(s)	996, 1256, 1463
BsaAI	YACGTR	1 cut(s)	415
BsaBI	GATNNNNATC	1 cut(s)	1302
BsaJI	CCNNGG	6 cut(s)	98, 352, 870, 1380, 1718, 1800
BsaWI	WCCGGW	1 cut(s)	910
BsaXI	ACNNNNNCTCC	4 cut(s)	1128, 1158, 1718, 1748
Bsc4I	CCNNNNNNNGG	5 cut(s)	238, 491, 620, 871, 1761
Bse118I	RCCGGY	2 cut(s)	766, 1761
Bse1I	ACTGG	4 cut(s)	55, 528, 879, 1122
Bse3DI	GCAATG	4 cut(s)	409, 1024, 1264, 1573
Bse8I	GATNNNNATC	1 cut(s)	1302
BseAI	TCCGGA	1 cut(s)	910
BseBI	CCWGG	4 cut(s)	99, 363, 394, 1834
BseDI	CCNNGG	6 cut(s)	98, 352, 870, 1380, 1718, 1800
BseGI	GGATG	6 cut(s)	458, 724, 801, 1756, 1793, 1810
BseJI	GATNNNNATC	1 cut(s)	1302
BseLI	CCNNNNNNNGG	5 cut(s)	238, 491, 620, 871, 1761
BseMI	GCAATG	4 cut(s)	409, 1024, 1264, 1573
BseMII	CTCAG	6 cut(s)	11, 203, 249, 297, 908, 1226
BseNI	ACTGG	4 cut(s)	55, 528, 879, 1122
BseRI	GAGGAG	4 cut(s)	510, 819, 1149, 1339
BseSI	GKGCMC	1 cut(s)	864
BsgI	GTGCAG	1 cut(s)	639
Bsh1236I	CGCG	1 cut(s)	1802
BshFI	GGCC	3 cut(s)	268, 351, 1084
BsiHKAI	GWGCWC	3 cut(s)	510, 864, 1064
BsiSI	CCGG	3 cut(s)	767, 911, 1762
BslFI	GGGAC	2 cut(s)	1858, 1879
BslI	CCNNNNNNNGG	5 cut(s)	238, 491, 620, 871, 1761
BsmAI	GTCTC	1 cut(s)	548
BsmFI	GGGAC	2 cut(s)	1858, 1879
BsnI	GGCC	3 cut(s)	268, 351, 1084
Bsp1286I	GDGCHC	4 cut(s)	510, 864, 1064, 1242
Bsp13I	TCCGGA	1 cut(s)	910
Bsp143I	GATC	7 cut(s)	292, 501, 643, 952, 1006, 1438, 1743
Bsp19I	CCATGG	1 cut(s)	870
BspACI	CCGC	8 cut(s)	5, 239, 254, 962, 1175, 1330, 1800, 1802
BspANI	GGCC	3 cut(s)	268, 351, 1084
BspCNI	CTCAG	7 cut(s)	4, 12, 204, 248, 298, 907, 1225
BspEI	TCCGGA	1 cut(s)	910
BspFNI	CGCG	1 cut(s)	1802
BspLI	GGNNCC	8 cut(s)	537, 579, 756, 1008, 1083, 1171, 1377, 1479
BspPI	GGATC	2 cut(s)	1001, 1014
BspQI	GCTCTTC	1 cut(s)	1052
BsrDI	GCAATG	4 cut(s)	409, 1024, 1264, 1573
BsrFI	RCCGGY	2 cut(s)	766, 1761
BsrI	ACTGG	4 cut(s)	55, 528, 879, 1122
BssAI	RCCGGY	2 cut(s)	766, 1761
BssECI	CCNNGG	6 cut(s)	98, 352, 870, 1380, 1718, 1800
BssMI	GATC	7 cut(s)	292, 501, 643, 952, 1006, 1438, 1743
BssT1I	CCWWGG	3 cut(s)	352, 870, 1718
Bst2UI	CCWGG	4 cut(s)	99, 363, 394, 1834
Bst4CI	ACNGT	6 cut(s)	157, 472, 1229, 1525, 1547, 1675
Bst6I	CTCTTC	4 cut(s)	1052, 1124, 1452, 1499
BstBAI	YACGTR	1 cut(s)	415
BstC8I	GCNNGC	7 cut(s)	33, 241, 258, 827, 962, 1763, 1878
BstDEI	CTNAG	8 cut(s)	12, 20, 212, 235, 306, 894, 933, 1212
BstDSI	CCRYGG	2 cut(s)	870, 1800
BstENI	CCTNNNNNAGG	1 cut(s)	618
BstF5I	GGATG	6 cut(s)	458, 724, 801, 1756, 1793, 1810
BstFNI	CGCG	1 cut(s)	1802
BstHHI	GCGC	1 cut(s)	458
BstKTI	GATC	7 cut(s)	295, 504, 646, 955, 1009, 1441, 1746
BstMAI	GTCTC	1 cut(s)	548
BstMBI	GATC	7 cut(s)	292, 501, 643, 952, 1006, 1438, 1743
BstMWI	GCNNNNNNNGC	3 cut(s)	245, 455, 826
BstNI	CCWGG	4 cut(s)	99, 363, 394, 1834
BstNSI	RCATGY	2 cut(s)	557, 1223
BstPAI	GACNNNNGTC	1 cut(s)	540
BstSCI	CCNGG	4 cut(s)	97, 361, 392, 1832
BstSFI	CTRYAG	1 cut(s)	1581
BstSLI	GKGCMC	1 cut(s)	864
BstUI	CGCG	1 cut(s)	1802
BstV2I	GAAGAC	1 cut(s)	331
BstX2I	RGATCY	3 cut(s)	292, 1006, 1438
BstYI	RGATCY	3 cut(s)	292, 1006, 1438
BsuI	GTATCC	3 cut(s)	62, 918, 1414
BsuRI	GGCC	3 cut(s)	268, 351, 1084
BtgI	CCRYGG	2 cut(s)	870, 1800
BtgZI	GCGATG	1 cut(s)	132
BtsCI	GGATG	6 cut(s)	458, 724, 801, 1756, 1793, 1810
BtsI	GCAGTG	1 cut(s)	955
BtsIMutI	CAGTG	3 cut(s)	62, 886, 955
Cac8I	GCNNGC	7 cut(s)	33, 241, 258, 827, 962, 1763, 1878
CaiI	CAGNNNCTG	2 cut(s)	1286, 1902
CfoI	GCGC	1 cut(s)	458
Cfr10I	RCCGGY	2 cut(s)	766, 1761
Cfr13I	GGNCC	5 cut(s)	78, 535, 755, 929, 1082
Cfr42I	CCGCGG	1 cut(s)	1803
Csp6I	GTAC	1 cut(s)	124
CviAII	CATG	9 cut(s)	554, 571, 658, 784, 871, 965, 1220, 1307, 1747
CviQI	GTAC	1 cut(s)	124
DdeI	CTNAG	8 cut(s)	12, 20, 212, 235, 306, 894, 933, 1212
DpnI	GATC	7 cut(s)	294, 503, 645, 954, 1008, 1440, 1745
DpnII	GATC	7 cut(s)	292, 501, 643, 952, 1006, 1438, 1743
DraIII	CACNNNGTG	1 cut(s)	415
EaeI	YGGCCR	1 cut(s)	349
Eam1104I	CTCTTC	4 cut(s)	1052, 1124, 1452, 1499
EarI	CTCTTC	4 cut(s)	1052, 1124, 1452, 1499
EciI	GGCGGA	1 cut(s)	1345
Ecl136II	GAGCTC	2 cut(s)	508, 1062
Eco130I	CCWWGG	3 cut(s)	352, 870, 1718
Eco147I	AGGCCT	1 cut(s)	268
Eco24I	GRGCYC	3 cut(s)	510, 1064, 1242
Eco47I	GGWCC	4 cut(s)	78, 535, 755, 929
Eco53kI	GAGCTC	2 cut(s)	508, 1062
EcoICRI	GAGCTC	2 cut(s)	508, 1062
EcoNI	CCTNNNNNAGG	1 cut(s)	618
EcoO109I	RGGNCCY	2 cut(s)	535, 755
EcoRI	GAATTC	2 cut(s)	1096, 1809
EcoRII	CCWGG	4 cut(s)	97, 361, 392, 1832
EcoT14I	CCWWGG	3 cut(s)	352, 870, 1718
EcoT22I	ATGCAT	2 cut(s)	1221, 1312
EcoT38I	GRGCYC	3 cut(s)	510, 1064, 1242
ErhI	CCWWGG	3 cut(s)	352, 870, 1718
FaeI	CATG	9 cut(s)	557, 574, 661, 787, 874, 968, 1223, 1310, 1750
FaqI	GGGAC	2 cut(s)	1858, 1879
FatI	CATG	9 cut(s)	553, 570, 657, 783, 870, 964, 1219, 1306, 1746
FauI	CCCGC	3 cut(s)	232, 969, 1182
FbaI	TGATCA	1 cut(s)	952
FblI	GTMKAC	1 cut(s)	1276
FokI	GGATG	6 cut(s)	445, 731, 808, 1763, 1780, 1817
FriOI	GRGCYC	3 cut(s)	510, 1064, 1242
FspBI	CTAG	7 cut(s)	261, 320, 446, 530, 540, 615, 1739
GlaI	GCGC	1 cut(s)	457
HaeIII	GGCC	3 cut(s)	268, 351, 1084
HapII	CCGG	3 cut(s)	767, 911, 1762
HhaI	GCGC	1 cut(s)	458
Hin1II	CATG	9 cut(s)	557, 574, 661, 787, 874, 968, 1223, 1310, 1750
Hin6I	GCGC	1 cut(s)	456
HinP1I	GCGC	1 cut(s)	456
HincII	GTYRAC	2 cut(s)	679, 1225
HindII	GTYRAC	2 cut(s)	679, 1225
HinfI	GANTC	5 cut(s)	227, 776, 875, 1109, 1466
HpaII	CCGG	3 cut(s)	767, 911, 1762
HphI	GGTGA	2 cut(s)	276, 720
Hpy166II	GTNNAC	6 cut(s)	440, 679, 862, 1225, 1277, 1588
Hpy188I	TCNGA	6 cut(s)	13, 468, 897, 1215, 1318, 1540
Hpy188III	TCNNGA	6 cut(s)	530, 710, 911, 1093, 1106, 1442
Hpy8I	GTNNAC	6 cut(s)	440, 679, 862, 1225, 1277, 1588
HpyAV	CCTTC	4 cut(s)	258, 279, 785, 1591
HpyCH4III	ACNGT	6 cut(s)	157, 472, 1229, 1525, 1547, 1675
HpyCH4IV	ACGT	2 cut(s)	414, 1608
HpyF10VI	GCNNNNNNNGC	3 cut(s)	245, 455, 826
HpyF3I	CTNAG	8 cut(s)	12, 20, 212, 235, 306, 894, 933, 1212
HpySE526I	ACGT	2 cut(s)	414, 1608
Hsp92II	CATG	9 cut(s)	557, 574, 661, 787, 874, 968, 1223, 1310, 1750
HspAI	GCGC	1 cut(s)	456
Kpn2I	TCCGGA	1 cut(s)	910
KroI	GCCGGC	1 cut(s)	1761
KroNI	GCCGGC	1 cut(s)	1763
Ksp22I	TGATCA	1 cut(s)	952
KspI	CCGCGG	1 cut(s)	1803
Kzo9I	GATC	7 cut(s)	292, 501, 643, 952, 1006, 1438, 1743
LguI	GCTCTTC	1 cut(s)	1052
LmnI	GCTCC	4 cut(s)	60, 583, 806, 1477
LweI	GCATC	8 cut(s)	166, 284, 467, 1206, 1408, 1741, 1765, 1865
MaeI	CTAG	7 cut(s)	261, 320, 446, 530, 540, 615, 1739
MaeII	ACGT	2 cut(s)	414, 1608
MaeIII	GTNAC	2 cut(s)	514, 721
MalI	GATC	7 cut(s)	294, 503, 645, 954, 1008, 1440, 1745
MboI	GATC	7 cut(s)	292, 501, 643, 952, 1006, 1438, 1743
MfeI	CAATTG	1 cut(s)	1070
MflI	RGATCY	3 cut(s)	292, 1006, 1438
MhlI	GDGCHC	4 cut(s)	510, 864, 1064, 1242
MlsI	TGGCCA	1 cut(s)	351
MluNI	TGGCCA	1 cut(s)	351
MmeI	TCCRAC	2 cut(s)	1106, 1443
Mox20I	TGGCCA	1 cut(s)	351
Mph1103I	ATGCAT	2 cut(s)	1221, 1312
MroI	TCCGGA	1 cut(s)	910
MroNI	GCCGGC	1 cut(s)	1761
MroXI	GAANNNNTTC	4 cut(s)	268, 525, 918, 1813
MscI	TGGCCA	1 cut(s)	351
MseI	TTAA	5 cut(s)	558, 689, 848, 1677, 1827
MslI	CAYNNNNRTG	1 cut(s)	1773
Msp20I	TGGCCA	1 cut(s)	351
MspA1I	CMGCKG	2 cut(s)	239, 1802
MspI	CCGG	3 cut(s)	767, 911, 1762
MspR9I	CCNGG	4 cut(s)	99, 363, 394, 1834
MunI	CAATTG	1 cut(s)	1070
MvaI	CCWGG	4 cut(s)	99, 363, 394, 1834
MvnI	CGCG	1 cut(s)	1802
MwoI	GCNNNNNNNGC	3 cut(s)	245, 455, 826
NaeI	GCCGGC	1 cut(s)	1763
NcoI	CCATGG	1 cut(s)	870
NdeII	GATC	7 cut(s)	292, 501, 643, 952, 1006, 1438, 1743
NgoMIV	GCCGGC	1 cut(s)	1761
NlaIII	CATG	9 cut(s)	557, 574, 661, 787, 874, 968, 1223, 1310, 1750
NlaIV	GGNNCC	8 cut(s)	537, 579, 756, 1008, 1083, 1171, 1377, 1479
NmuCI	GTSAC	2 cut(s)	514, 721
NsiI	ATGCAT	2 cut(s)	1221, 1312
NspI	RCATGY	2 cut(s)	557, 1223
PceI	AGGCCT	1 cut(s)	268
PciSI	GCTCTTC	1 cut(s)	1052
PdiI	GCCGGC	1 cut(s)	1763
PdmI	GAANNNNTTC	4 cut(s)	268, 525, 918, 1813
PfeI	GAWTC	5 cut(s)	227, 776, 875, 1109, 1466
PflMI	CCANNNNNTGG	1 cut(s)	871
Ppu21I	YACGTR	1 cut(s)	415
PpuMI	RGGWCCY	2 cut(s)	535, 755
PshAI	GACNNNNGTC	1 cut(s)	540
PshBI	ATTAAT	1 cut(s)	689
Psp124BI	GAGCTC	2 cut(s)	510, 1064
Psp1406I	AACGTT	1 cut(s)	1608
Psp5II	RGGWCCY	2 cut(s)	535, 755
Psp6I	CCWGG	4 cut(s)	97, 361, 392, 1832
PspGI	CCWGG	4 cut(s)	97, 361, 392, 1832
PspN4I	GGNNCC	8 cut(s)	537, 579, 756, 1008, 1083, 1171, 1377, 1479
PspPI	GGNCC	5 cut(s)	78, 535, 755, 929, 1082
PspPPI	RGGWCCY	2 cut(s)	535, 755
PstNI	CAGNNNCTG	2 cut(s)	1286, 1902
PsuI	RGATCY	3 cut(s)	292, 1006, 1438
RsaI	GTAC	1 cut(s)	125
RsaNI	GTAC	1 cut(s)	124
RseI	CAYNNNNRTG	1 cut(s)	1773
SacI	GAGCTC	2 cut(s)	510, 1064
SacII	CCGCGG	1 cut(s)	1803
SapI	GCTCTTC	1 cut(s)	1052
SaqAI	TTAA	5 cut(s)	558, 689, 848, 1677, 1827
Sau3AI	GATC	7 cut(s)	292, 501, 643, 952, 1006, 1438, 1743
Sau96I	GGNCC	5 cut(s)	78, 535, 755, 929, 1082
ScrFI	CCNGG	4 cut(s)	99, 363, 394, 1834
SduI	GDGCHC	4 cut(s)	510, 864, 1064, 1242
SfaNI	GCATC	8 cut(s)	166, 284, 467, 1206, 1408, 1741, 1765, 1865
SfcI	CTRYAG	1 cut(s)	1581
Sfr303I	CCGCGG	1 cut(s)	1803
SgrBI	CCGCGG	1 cut(s)	1803
SinI	GGWCC	4 cut(s)	78, 535, 755, 929
SmiMI	CAYNNNNRTG	1 cut(s)	1773
SmlI	CTYRAG	3 cut(s)	1011, 1235, 1442
SmoI	CTYRAG	3 cut(s)	1011, 1235, 1442
SseBI	AGGCCT	1 cut(s)	268
SsiI	CCGC	8 cut(s)	5, 239, 254, 962, 1175, 1330, 1800, 1802
SspMI	CTAG	7 cut(s)	261, 320, 446, 530, 540, 615, 1739
SstI	GAGCTC	2 cut(s)	510, 1064
StuI	AGGCCT	1 cut(s)	268
StyD4I	CCNGG	4 cut(s)	97, 361, 392, 1832
StyI	CCWWGG	3 cut(s)	352, 870, 1718
TaaI	ACNGT	6 cut(s)	157, 472, 1229, 1525, 1547, 1675
TaiI	ACGT	2 cut(s)	417, 1611
TaqI	TCGA	2 cut(s)	504, 1646
TfiI	GAWTC	5 cut(s)	227, 776, 875, 1109, 1466
Tru1I	TTAA	5 cut(s)	558, 689, 848, 1677, 1827
Tru9I	TTAA	5 cut(s)	558, 689, 848, 1677, 1827
TscAI	CASTG	3 cut(s)	62, 886, 962
TseFI	GTSAC	2 cut(s)	514, 721
Tsp45I	GTSAC	2 cut(s)	514, 721
TspGWI	ACGGA	1 cut(s)	816
TspRI	CASTG	3 cut(s)	62, 886, 962
Van91I	CCANNNNNTGG	1 cut(s)	871
VneI	GTGCAC	1 cut(s)	860
VpaK11BI	GGWCC	4 cut(s)	78, 535, 755, 929
VspI	ATTAAT	1 cut(s)	689
XagI	CCTNNNNNAGG	1 cut(s)	618
XapI	RAATTY	4 cut(s)	69, 1096, 1486, 1809
XbaI	TCTAGA	1 cut(s)	529
XceI	RCATGY	2 cut(s)	557, 1223
XcmI	CCANNNNNNNNNTGG	1 cut(s)	62
XmiI	GTMKAC	1 cut(s)	1276
XmnI	GAANNNNTTC	4 cut(s)	268, 525, 918, 1813
XspI	CTAG	7 cut(s)	261, 320, 446, 530, 540, 615, 1739
Zsp2I	ATGCAT	2 cut(s)	1221, 1312

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.