Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0011834

Physical Location & Seq

Genomic Coordinates Forward (+)

24910331 .. 24916163

Gene Structure 5833 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1109500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2847 bp

ATGGCATCCATTGACATTCTCAATCCACCCAGAATTTGCATTCCCAAACCCCACACCCACTTCAAATCCCCAGCTCACTCGAAGCGTTTCTGTTTGGTTCGGAAACTTCAACCGCAGCTGCCTCTCCGCCACCGGTCACTCACCCTTATCTGCCAAACGTCTTCCGGGCCGTCGTCAAGGTCCGGTGACAATAGCAAATCCCCCCAGGACGATTTTGTGGCCAGGGTTTTGAAGGAGAACCCTAGCCAGATTGAACCCAGGTACTTGGTTGGCGACAAGTTCTATACTTTGAAGGAAAAAGAGAGTTTGGGGAAGAATTCCAATGTGGGTTTTGTTGAACTCTTGGCCAAACAGCTAAATTTTTCGAAGCTGAAAAATGAGAGAATTGAGGGACAGAGTGATGGTGGAGTGAAGGATGATGGTGTGTATTTGAAGGACATTTTGAGGGAGTATAAGGGGAAGCTCTATGTGCCTGAGCAGATTTTTGGGACGGAGTTGCCAGAGGAAGAGCAATTTGAGAAGAGTTTGGAGGAATTGCCCAGAATGAGCTATGAGGATTTTCTTAAAGCTATGAAGAGTGAAAAGGTTAAGCTCTTGACTTCCAAGGAGGTTGCCGGGACTTCTTATGGGGTCAGTGATTACATTGTTGATTTGAAGGAGATACCGGGCGAAAAGAGCTTGCATCGAACCAAATGGGCAATGAGGCTGGATGAGGGTGAAGCTCAAGCTCTTTTGGAGGATTACACGGGCCCGAGATATGTGATTGAGGGGCATACTACGTCTTGGGTTGGAAAGGTACCACAGTACCCTCATCCGGTGGCATCTTCCATATCTAGCAGAATGATGGTGGAGCTTGGAATGGTAACAGCTGTAATGGCTGCTGCAGCAGTTTTTATTGGAGGATTCCTGGCTTCTGCAGTATTTGCTGTTACCAGCTTCGTTTTTGCGTCAACTGTATATGTCGTATGGCCTATAGTGAAACCATTTACGAGACTTTTCCTTGGTCTCGCCTTGGGTATTTTGGAGAGAGTATGGGATAACCTTGTTGACTTTTTCAGTGATGGAGGCATTTTCTCTAAATTTTCTGAATTTTACACTTTTGGTGGTCTATCTGCCAGCATTGAGATGTTAAAACCAATAACGATTGTCCTTATGACCATGGTCATTCTTGTCCGTTTCACACTTTCAAGAAGACCTAAGAACTTCCGGAAGTGGGATCTGTGGCAAGGGATTGATTTTTCACGATCCAAAGCAGAAGCCCGTGTTGATGGGTCAACCGGAGTTAAGTTTAGTGATGTGGCAGGGATTGATGAAGCAGTAGAGGAACTTCTGGAGTTGGTGAGGTACCTGAAGAACCCAGAACTATTTGACAAAATGGGAATAAAACCTCCGCATGGGGTTCTTTTAGAGGGTCCACCTGGATGTGGCAAGACCCTGGTTGCGAAGGCCATAGCTGGTGAGGCTGGTGTTCCGTTTTACCAAATGGCTGGATCTGAATTTGTTGAAGTCTTAGTTGGTGTTGGTTCAGCTCGTATTAGGGATCTATTCAAAAGAGCCAAGGTAAACAAACCATCTGTTATTTTCATTGATGAGATTGATGCATTGGCTACGAGGCGTCAAGGAATTTTCAAGGAAACTAGCGACCACCTGTATAATGCAGCCACTCAAGAGAGAGAAACTACTCTGAATCAGCTCTTAATAGAGCTTGATGGATTTGATACTGGTAAAGGTGTTATATTCTTAGCTGCTACAAATCGCCGGGATTTATTAGACCCTGCACTTCTTCGACCAGGTCGTTTTGATCGGAAGATAAAAATTCGTCCACCTGCTGCGAAGGGGAGATTGGATATTTTGAAAATCCATGCAAGCAAAGTCAAAATGTCACCATCTGTTGATTTGTCCAGCTATGCACAGAACTTACCAGGATGGACGGGAGCAAAGTTGGCTCAGCTGGTCCAAGAGGCTGCACTTGTCGCAGTGAGGAAGGGGCATGATTCAATTTTTCAGACAGACTTGGATGATGCTGTTGATAGACTCACTGTAGGACCAAAGCGGGTTGGAATAGAGTTGGGCCATCTGGGTCAATGTCGTCGAGCCACTACTGAAGTTGGAGTGGCAATGACTTCTCATCTGCTTAGGCAATACGAAAACGCAGAAGTTGAACGGTGTGATCGCATTTCAATCATCCCTCGTGGTCAGACATTGTCACAAGTTGTATTTCATCGACTTGATGACGAATCATACATGTTTGAGCGCCGGCCACAATTGCTGCATCGCCTACAGGTTTTACTTGGTGGAAGGGCCGCTGAAGAGGTCATATATGGACGAGACTCATCAAGGGCATCGGTTGACTACCTTGCTGATGCATCTTGGCTTGCTCGTAAAATTTTAACCATTTGGAATTTGGAGAATCCGATGGTCATACATGGAGAGCCACCACCTTGGAGGAAGAAACTTGAATTTGTAGGCCCTCGGCTGGACTTCGAGGGATCACTGTACCACGACTATGACCTGATTGAACCACCAGTGAACTTCAATTTAGATGATGATGTTGCAAAAAGGGCGGAAGAGCTGATACACAAAATGTATGACAAAACACTTTCTCTGCTTAAGAAGCATCACACGGCCTTGCTTAAAACTGTGAAGGTTCTTCTTGAACGCAAGGAAATTAGCGGTGAAGAAATTGATTTTATCTTGAACAAGTACCTTCCACAAACACCCTTAAAGCTTCTTTTAGAGGAGGAAAATCCTGGCAGCCTTAAGTTCGTCACACAAGAACAGGAACAGGAACAGGAACGTGAGTCAGAGTACGCCCTTCTATCTCAATCCAATGGAGAAACCCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009657 GO:0009658 GO:0009706 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010020 GO:0010154 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0019866 GO:0022414 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0042170 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

949

Amino Acids

106.61

Weight (kDa)

6.89

Isoelectric Point (pI)

39.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	467 - 607	1.8e-39	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	630 - 673	1.5e-07	AAA+ lid domain
Peptidase_M41	PF01434	717 - 802	1.5e-11	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011834)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23940
fragaria_vesca	FvH4_5g04870 FvH4_5g04870
malus_domestica	MD06G1109500.v1.1 MD14G1130500.v1.1
prunus_persica	Prupe.5G122500_v2.0.a1
pyrus_communis	pycom06g10310
rosa_chinensis	RchiOBHm_Chr7g0194221
rosa_laevigata	RLG00000004209
rosa_multiflora	Rmu_co8268851.1_g000001 Rmu_sc0000901.1_g000001 Rmu_sc0009433.1_g000015
rosa_roxburghii	Rroxscaffold_3G00261490
rosa_rugosa	Rorug07G0014400
rosa_samantha	Rh7AG141800 Rh7BG142200 Rh7CG145900 Rh7DG143200
rosa_wichuraiana	Rw7G012120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1834
Acc36I	ACCTGC	1 cut(s)	1834
Acc65I	GGTACC	2 cut(s)	796, 1344
AccB1I	GGYRCC	2 cut(s)	796, 1344
AccIII	TCCGGA	1 cut(s)	1206
AciI	CCGC	7 cut(s)	113, 127, 1391, 2053, 2304, 2564, 2673
AclWI	GGATC	5 cut(s)	1224, 1239, 1498, 1548, 2497
AcoI	YGGCCR	3 cut(s)	219, 345, 2258
AcuI	CTGAAG	3 cut(s)	1370, 2124, 2328
AcyI	GRCGYC	1 cut(s)	1615
AfaI	GTAC	7 cut(s)	263, 798, 806, 1346, 2498, 2705, 2810
AfiI	CCNNNNNNNGG	5 cut(s)	814, 1213, 1394, 1424, 2476
AflII	CTTAAG	2 cut(s)	2609, 2759
AflIII	ACRYGT	1 cut(s)	2244
AgeI	ACCGGT	1 cut(s)	132
AjnI	CCWGG	9 cut(s)	204, 221, 257, 906, 1417, 1434, 1789, 1921, 2749
AjuI	GAANNNNNNNTTGG	4 cut(s)	1497, 1529, 1826, 1858
Alw26I	GTCTC	3 cut(s)	985, 1010, 2322
AlwI	GGATC	5 cut(s)	1224, 1239, 1498, 1548, 2497
Ama87I	CYCGRG	1 cut(s)	751
Aor13HI	TCCGGA	1 cut(s)	1206
ApaI	GGGCCC	1 cut(s)	752
AsiGI	ACCGGT	1 cut(s)	132
Asp700I	GAANNNNTTC	1 cut(s)	86
Asp718I	GGTACC	2 cut(s)	796, 1344
AspLEI	GCGC	1 cut(s)	2256
AsuC2I	CCSGG	4 cut(s)	166, 616, 666, 1760
AsuHPI	GGTGA	7 cut(s)	133, 197, 728, 1351, 1469, 1875, 2687
AsuII	TTCGAA	1 cut(s)	365
AvaI	CYCGRG	1 cut(s)	751
AvaII	GGWCC	4 cut(s)	180, 1412, 1954, 2045
BaeGI	GKGCMC	1 cut(s)	752
BaeI	ACNNNNGTAYC	4 cut(s)	788, 788, 821, 821
BalI	TGGCCA	2 cut(s)	221, 347
BanI	GGYRCC	2 cut(s)	796, 1344
BanII	GRGCYC	1 cut(s)	752
BarI	GAAGNNNNNNTAC	4 cut(s)	1311, 1343, 2559, 2591
BauI	CACGAG	1 cut(s)	2190
BbsI	GAAGAC	2 cut(s)	153, 1198
BceAI	ACGGC	2 cut(s)	154, 2640
BciT130I	CCWGG	9 cut(s)	206, 223, 259, 908, 1419, 1436, 1791, 1923, 2751
BcnI	CCSGG	4 cut(s)	166, 616, 666, 1760
BcoDI	GTCTC	3 cut(s)	985, 1010, 2322
BfaI	CTAG	3 cut(s)	243, 834, 1638
BfmI	CTRYAG	5 cut(s)	882, 915, 972, 2040, 2279
BfoI	RGCGCY	1 cut(s)	2257
BfrI	CTTAAG	2 cut(s)	2609, 2759
BfuAI	ACCTGC	1 cut(s)	1834
BlpI	GCTNAGC	1 cut(s)	1947
Bme18I	GGWCC	4 cut(s)	180, 1412, 1954, 2045
BmeT110I	CYCGRG	1 cut(s)	751
BmiI	GGNNCC	4 cut(s)	750, 798, 1346, 1413
BoxI	GACNNNNGTC	1 cut(s)	1160
BpiI	GAAGAC	2 cut(s)	153, 1198
BpmI	CTGGAG	1 cut(s)	1352
Bpu10I	CCTNAGC	2 cut(s)	474, 2135
Bpu1102I	GCTNAGC	1 cut(s)	1947
Bpu14I	TTCGAA	1 cut(s)	365
BpuEI	CTTGAG	2 cut(s)	708, 1650
BpuMI	CCSGG	4 cut(s)	166, 616, 666, 1760
BsaHI	GRCGYC	1 cut(s)	1615
BsaI	GGTCTC	1 cut(s)	1010
BsaWI	WCCGGW	5 cut(s)	132, 182, 814, 1206, 1277
Bsc4I	CCNNNNNNNGG	5 cut(s)	814, 1213, 1394, 1424, 2476
Bse118I	RCCGGY	2 cut(s)	132, 2256
Bse1I	ACTGG	2 cut(s)	1726, 2525
Bse3DI	GCAATG	2 cut(s)	705, 2124
BseAI	TCCGGA	1 cut(s)	1206
BseBI	CCWGG	9 cut(s)	206, 223, 259, 908, 1419, 1436, 1791, 1923, 2751
BseGI	GGATG	8 cut(s)	5, 421, 715, 811, 1427, 1931, 2023, 2184
BseLI	CCNNNNNNNGG	5 cut(s)	814, 1213, 1394, 1424, 2476
BseMI	GCAATG	2 cut(s)	705, 2124
BseMII	CTCAG	2 cut(s)	465, 1961
BseNI	ACTGG	2 cut(s)	1726, 2525
BseRI	GAGGAG	1 cut(s)	2753
BseSI	GKGCMC	1 cut(s)	752
BseYI	CCCAGC	1 cut(s)	70
BsgI	GTGCAG	2 cut(s)	1761, 1950
BshNI	GGYRCC	2 cut(s)	796, 1344
BshTI	ACCGGT	1 cut(s)	132
BsiHKCI	CYCGRG	1 cut(s)	751
BslFI	GGGAC	3 cut(s)	405, 502, 631
BslI	CCNNNNNNNGG	5 cut(s)	814, 1213, 1394, 1424, 2476
BsmAI	GTCTC	3 cut(s)	985, 1010, 2322
BsmFI	GGGAC	3 cut(s)	405, 502, 631
BsmI	GAATGC	1 cut(s)	39
Bso31I	GGTCTC	1 cut(s)	1010
BsoBI	CYCGRG	1 cut(s)	751
Bsp119I	TTCGAA	1 cut(s)	365
Bsp120I	GGGCCC	1 cut(s)	748
Bsp1286I	GDGCHC	1 cut(s)	752
Bsp13I	TCCGGA	1 cut(s)	1206
Bsp143I	GATC	7 cut(s)	1216, 1244, 1490, 1540, 1801, 2170, 2489
Bsp1720I	GCTNAGC	1 cut(s)	1947
Bsp19I	CCATGG	1 cut(s)	1158
BspACI	CCGC	7 cut(s)	113, 127, 1391, 2053, 2304, 2564, 2673
BspCNI	CTCAG	2 cut(s)	466, 1960
BspEI	TCCGGA	1 cut(s)	1206
BspLI	GGNNCC	4 cut(s)	750, 798, 1346, 1413
BspMAI	CTGCAG	2 cut(s)	886, 919
BspMI	ACCTGC	1 cut(s)	1834
BspPI	GGATC	5 cut(s)	1224, 1239, 1498, 1548, 2497
BspQI	GCTCTTC	2 cut(s)	501, 2562
BspT104I	TTCGAA	1 cut(s)	365
BspT107I	GGYRCC	2 cut(s)	796, 1344
BspTI	CTTAAG	2 cut(s)	2609, 2759
BspTNI	GGTCTC	1 cut(s)	1010
BsrDI	GCAATG	2 cut(s)	705, 2124
BsrFI	RCCGGY	2 cut(s)	132, 2256
BsrI	ACTGG	2 cut(s)	1726, 2525
BssAI	RCCGGY	2 cut(s)	132, 2256
BssMI	GATC	7 cut(s)	1216, 1244, 1490, 1540, 1801, 2170, 2489
BssNI	GRCGYC	1 cut(s)	1615
BssSI	CACGAG	1 cut(s)	2190
BssT1I	CCWWGG	6 cut(s)	603, 1000, 1011, 1158, 1557, 2441
Bst2BI	CACGAG	1 cut(s)	2190
Bst2UI	CCWGG	9 cut(s)	206, 223, 259, 908, 1419, 1436, 1791, 1923, 2751
Bst4CI	ACNGT	6 cut(s)	804, 955, 2041, 2166, 2496, 2641
Bst6I	CTCTTC	5 cut(s)	501, 515, 569, 2304, 2562
BstACI	GRCGYC	1 cut(s)	1615
BstAFI	CTTAAG	2 cut(s)	2609, 2759
BstAPI	GCANNNNNTGC	1 cut(s)	923
BstBI	TTCGAA	1 cut(s)	365
BstC8I	GCNNGC	5 cut(s)	680, 1117, 1867, 2258, 2376
BstDEI	CTNAG	6 cut(s)	474, 1197, 1510, 1741, 1947, 2135
BstDSI	CCRYGG	1 cut(s)	1158
BstF5I	GGATG	8 cut(s)	5, 421, 715, 811, 1427, 1931, 2023, 2184
BstH2I	RGCGCY	1 cut(s)	2257
BstHHI	GCGC	1 cut(s)	2256
BstKTI	GATC	7 cut(s)	1219, 1247, 1493, 1543, 1804, 2173, 2492
BstMAI	GTCTC	3 cut(s)	985, 1010, 2322
BstMBI	GATC	7 cut(s)	1216, 1244, 1490, 1540, 1801, 2170, 2489
BstNI	CCWGG	9 cut(s)	206, 223, 259, 908, 1419, 1436, 1791, 1923, 2751
BstNSI	RCATGY	1 cut(s)	2248
BstPAI	GACNNNNGTC	1 cut(s)	1160
BstSFI	CTRYAG	5 cut(s)	882, 915, 972, 2040, 2279
BstSLI	GKGCMC	1 cut(s)	752
BstV2I	GAAGAC	2 cut(s)	153, 1198
BstX2I	RGATCY	3 cut(s)	1216, 1490, 1540
BstXI	CCANNNNNNTGG	3 cut(s)	265, 1487, 2442
BstYI	RGATCY	3 cut(s)	1216, 1490, 1540
BtgI	CCRYGG	1 cut(s)	1158
BtgZI	GCGATG	1 cut(s)	2258
BtsCI	GGATG	8 cut(s)	5, 421, 715, 811, 1427, 1931, 2023, 2184
BtsI	GCAGTG	1 cut(s)	1983
BtsIMutI	CAGTG	6 cut(s)	640, 1063, 1983, 2037, 2492, 2532
BveI	ACCTGC	1 cut(s)	1834
Cac8I	GCNNGC	5 cut(s)	680, 1117, 1867, 2258, 2376
CfoI	GCGC	1 cut(s)	2256
Cfr10I	RCCGGY	2 cut(s)	132, 2256
CseI	GACGC	2 cut(s)	936, 1604
CsiI	ACCWGGT	1 cut(s)	1789
Csp6I	GTAC	7 cut(s)	262, 797, 805, 1345, 2497, 2704, 2809
CspAI	ACCGGT	1 cut(s)	132
CviAII	CATG	6 cut(s)	1159, 1394, 1862, 1991, 2245, 2426
CviQI	GTAC	7 cut(s)	262, 797, 805, 1345, 2497, 2704, 2809
DdeI	CTNAG	6 cut(s)	474, 1197, 1510, 1741, 1947, 2135
DpnI	GATC	7 cut(s)	1218, 1246, 1492, 1542, 1803, 2172, 2491
DpnII	GATC	7 cut(s)	1216, 1244, 1490, 1540, 1801, 2170, 2489
EaeI	YGGCCR	3 cut(s)	219, 345, 2258
Eam1104I	CTCTTC	5 cut(s)	501, 515, 569, 2304, 2562
EarI	CTCTTC	5 cut(s)	501, 515, 569, 2304, 2562
EciI	GGCGGA	2 cut(s)	116, 2579
Eco130I	CCWWGG	6 cut(s)	603, 1000, 1011, 1158, 1557, 2441
Eco24I	GRGCYC	1 cut(s)	752
Eco31I	GGTCTC	1 cut(s)	1010
Eco47I	GGWCC	4 cut(s)	180, 1412, 1954, 2045
Eco57I	CTGAAG	3 cut(s)	1370, 2124, 2328
Eco88I	CYCGRG	1 cut(s)	751
EcoO109I	RGGNCCY	1 cut(s)	2468
EcoRI	GAATTC	1 cut(s)	316
EcoRII	CCWGG	9 cut(s)	204, 221, 257, 906, 1417, 1434, 1789, 1921, 2749
EcoT14I	CCWWGG	6 cut(s)	603, 1000, 1011, 1158, 1557, 2441
EcoT22I	ATGCAT	2 cut(s)	1603, 2368
EcoT38I	GRGCYC	1 cut(s)	752
ErhI	CCWWGG	6 cut(s)	603, 1000, 1011, 1158, 1557, 2441
FaeI	CATG	6 cut(s)	1162, 1397, 1865, 1994, 2248, 2429
FaqI	GGGAC	3 cut(s)	405, 502, 631
FatI	CATG	6 cut(s)	1158, 1393, 1861, 1990, 2244, 2425
FauI	CCCGC	1 cut(s)	2046
FokI	GGATG	7 cut(s)	428, 722, 798, 1434, 1938, 2030, 2171
FriOI	GRGCYC	1 cut(s)	752
FspBI	CTAG	3 cut(s)	243, 834, 1638
GlaI	GCGC	1 cut(s)	2255
GsaI	CCCAGC	1 cut(s)	74
GsuI	CTGGAG	1 cut(s)	1352
HaeII	RGCGCY	1 cut(s)	2257
HgaI	GACGC	2 cut(s)	936, 1604
HhaI	GCGC	1 cut(s)	2256
Hin1I	GRCGYC	1 cut(s)	1615
Hin1II	CATG	6 cut(s)	1162, 1397, 1865, 1994, 2248, 2429
Hin6I	GCGC	1 cut(s)	2254
HinP1I	GCGC	1 cut(s)	2254
HincII	GTYRAC	4 cut(s)	951, 1048, 1275, 2350
HindII	GTYRAC	4 cut(s)	951, 1048, 1275, 2350
HindIII	AAGCTT	1 cut(s)	2726
HinfI	GANTC	8 cut(s)	903, 1687, 1994, 2034, 2237, 2330, 2410, 2801
HphI	GGTGA	7 cut(s)	133, 197, 728, 1351, 1469, 1875, 2687
Hpy166II	GTNNAC	8 cut(s)	951, 1048, 1275, 1415, 1564, 1823, 2350, 2530
Hpy188I	TCNGA	9 cut(s)	102, 1087, 1495, 1686, 1806, 2007, 2199, 2415, 2806
Hpy188III	TCNNGA	8 cut(s)	595, 1188, 1207, 1242, 1331, 1667, 2654, 2695
Hpy8I	GTNNAC	8 cut(s)	951, 1048, 1275, 1415, 1564, 1823, 2350, 2530
Hpy99I	CGWCG	2 cut(s)	175, 2094
HpyCH4III	ACNGT	6 cut(s)	804, 955, 2041, 2166, 2496, 2641
HpyCH4IV	ACGT	3 cut(s)	158, 779, 2797
HpyF3I	CTNAG	6 cut(s)	474, 1197, 1510, 1741, 1947, 2135
HpySE526I	ACGT	3 cut(s)	158, 779, 2797
Hsp92I	GRCGYC	1 cut(s)	1615
Hsp92II	CATG	6 cut(s)	1162, 1397, 1865, 1994, 2248, 2429
HspAI	GCGC	1 cut(s)	2254
Kpn2I	TCCGGA	1 cut(s)	1206
KpnI	GGTACC	2 cut(s)	800, 1348
KroI	GCCGGC	1 cut(s)	2256
KroNI	GCCGGC	1 cut(s)	2258
Kzo9I	GATC	7 cut(s)	1216, 1244, 1490, 1540, 1801, 2170, 2489
LguI	GCTCTTC	2 cut(s)	501, 2562
LmnI	GCTCC	2 cut(s)	850, 1934
MabI	ACCWGGT	1 cut(s)	1789
MaeI	CTAG	3 cut(s)	243, 834, 1638
MaeII	ACGT	3 cut(s)	158, 779, 2797
MaeIII	GTNAC	7 cut(s)	135, 185, 862, 928, 1881, 2205, 2767
MalI	GATC	7 cut(s)	1218, 1246, 1492, 1542, 1803, 2172, 2491
MboI	GATC	7 cut(s)	1216, 1244, 1490, 1540, 1801, 2170, 2489
MfeI	CAATTG	1 cut(s)	2264
MflI	RGATCY	3 cut(s)	1216, 1490, 1540
MhlI	GDGCHC	1 cut(s)	752
MlsI	TGGCCA	2 cut(s)	221, 347
MluNI	TGGCCA	2 cut(s)	221, 347
MlyI	GAGTC	3 cut(s)	2028, 2324, 2810
MmeI	TCCRAC	3 cut(s)	769, 2038, 2089
Mox20I	TGGCCA	2 cut(s)	221, 347
Mph1103I	ATGCAT	2 cut(s)	1603, 2368
MroI	TCCGGA	1 cut(s)	1206
MroNI	GCCGGC	1 cut(s)	2256
MroXI	GAANNNNTTC	1 cut(s)	86
MscI	TGGCCA	2 cut(s)	221, 347
MslI	CAYNNNNRTG	3 cut(s)	1124, 1420, 2505
Msp20I	TGGCCA	2 cut(s)	221, 347
MspA1I	CMGCKG	4 cut(s)	118, 869, 1951, 2306
MspCI	CTTAAG	2 cut(s)	2609, 2759
MunI	CAATTG	1 cut(s)	2264
Mva1269I	GAATGC	1 cut(s)	39
MvaI	CCWGG	9 cut(s)	206, 223, 259, 908, 1419, 1436, 1791, 1923, 2751
NaeI	GCCGGC	1 cut(s)	2258
NciI	CCSGG	4 cut(s)	166, 616, 666, 1760
NcoI	CCATGG	1 cut(s)	1158
NdeII	GATC	7 cut(s)	1216, 1244, 1490, 1540, 1801, 2170, 2489
NgoMIV	GCCGGC	1 cut(s)	2256
NlaIII	CATG	6 cut(s)	1162, 1397, 1865, 1994, 2248, 2429
NlaIV	GGNNCC	4 cut(s)	750, 798, 1346, 1413
NmeAIII	GCCGAG	1 cut(s)	2452
NmuCI	GTSAC	5 cut(s)	135, 185, 1881, 2205, 2767
NsiI	ATGCAT	2 cut(s)	1603, 2368
NspI	RCATGY	1 cut(s)	2248
NspV	TTCGAA	1 cut(s)	365
PaqCI	CACCTGC	1 cut(s)	1834
PciI	ACATGT	1 cut(s)	2244
PciSI	GCTCTTC	2 cut(s)	501, 2562
PcsI	WCGNNNNNNNCGW	1 cut(s)	1792
PctI	GAATGC	1 cut(s)	39
PdiI	GCCGGC	1 cut(s)	2258
PdmI	GAANNNNTTC	1 cut(s)	86
PfeI	GAWTC	5 cut(s)	903, 1687, 1994, 2237, 2410
PflFI	GACNNNGTC	2 cut(s)	1791, 2203
PinAI	ACCGGT	1 cut(s)	132
PleI	GAGTC	3 cut(s)	2028, 2324, 2809
PpsI	GAGTC	3 cut(s)	2028, 2324, 2809
PscI	ACATGT	1 cut(s)	2244
PshAI	GACNNNNGTC	1 cut(s)	1160
Psp6I	CCWGG	9 cut(s)	204, 221, 257, 906, 1417, 1434, 1789, 1921, 2749
PspFI	CCCAGC	1 cut(s)	70
PspGI	CCWGG	9 cut(s)	204, 221, 257, 906, 1417, 1434, 1789, 1921, 2749
PspN4I	GGNNCC	4 cut(s)	750, 798, 1346, 1413
PspOMI	GGGCCC	1 cut(s)	748
PstI	CTGCAG	2 cut(s)	886, 919
PsuI	RGATCY	3 cut(s)	1216, 1490, 1540
PsyI	GACNNNGTC	2 cut(s)	1791, 2203
PvuII	CAGCTG	3 cut(s)	118, 869, 1951
RsaI	GTAC	7 cut(s)	263, 798, 806, 1346, 2498, 2705, 2810
RsaNI	GTAC	7 cut(s)	262, 797, 805, 1345, 2497, 2704, 2809
RseI	CAYNNNNRTG	3 cut(s)	1124, 1420, 2505
SapI	GCTCTTC	2 cut(s)	501, 2562
Sau3AI	GATC	7 cut(s)	1216, 1244, 1490, 1540, 1801, 2170, 2489
SchI	GAGTC	3 cut(s)	2028, 2324, 2810
SduI	GDGCHC	1 cut(s)	752
SexAI	ACCWGGT	1 cut(s)	1789
SfcI	CTRYAG	5 cut(s)	882, 915, 972, 2040, 2279
SfuI	TTCGAA	1 cut(s)	365
SinI	GGWCC	4 cut(s)	180, 1412, 1954, 2045
SmiMI	CAYNNNNRTG	3 cut(s)	1124, 1420, 2505
SmlI	CTYRAG	4 cut(s)	723, 1665, 2609, 2759
SmoI	CTYRAG	4 cut(s)	723, 1665, 2609, 2759
SsiI	CCGC	7 cut(s)	113, 127, 1391, 2053, 2304, 2564, 2673
SspMI	CTAG	3 cut(s)	243, 834, 1638
StyI	CCWWGG	6 cut(s)	603, 1000, 1011, 1158, 1557, 2441
TaaI	ACNGT	6 cut(s)	804, 955, 2041, 2166, 2496, 2641
TaiI	ACGT	3 cut(s)	161, 782, 2800
TaqI	TCGA	7 cut(s)	80, 365, 685, 1786, 2092, 2224, 2484
TauI	GCSGC	1 cut(s)	2306
TfiI	GAWTC	5 cut(s)	903, 1687, 1994, 2237, 2410
TscAI	CASTG	6 cut(s)	640, 1063, 1983, 2044, 2499, 2532
TseFI	GTSAC	5 cut(s)	135, 185, 1881, 2205, 2767
Tsp45I	GTSAC	5 cut(s)	135, 185, 1881, 2205, 2767
TspDTI	ATGAA	4 cut(s)	587, 1326, 1573, 2210
TspGWI	ACGGA	3 cut(s)	506, 1163, 1461
TspRI	CASTG	6 cut(s)	640, 1063, 1983, 2044, 2499, 2532
Tth111I	GACNNNGTC	2 cut(s)	1791, 2203
Vha464I	CTTAAG	2 cut(s)	2609, 2759
VpaK11BI	GGWCC	4 cut(s)	180, 1412, 1954, 2045
XceI	RCATGY	1 cut(s)	2248
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2401
XmnI	GAANNNNTTC	1 cut(s)	86
XspI	CTAG	3 cut(s)	243, 834, 1638
Zsp2I	ATGCAT	2 cut(s)	1603, 2368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.