Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0011834

Physical Location & Seq

Genomic Coordinates Forward (+)

20826328 .. 20832383

Gene Structure 6056 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1130500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2847 bp

ATGGCATCCATTGACATTCTCAATCCACCCAAAATTTACATTCCCAAACCACACACCCACTTCAAACCCCCAGCTCAGTCGAAGCGTTTCGGTTTGATTCGGAAAGTTCAACCGCAGCTGCCTATCCGCCACCGGTCACTCACCCTCCTCTGCCAATCGTCTTCCTGGCCGTCGTCAAGGTCCGGTGACAATAGCAAAGCCCCCCAGGACGATTATGTGACCAGGGTCTTGAAGGAGAACCCTAGCCAGATTGAACCCAGGTACTTGGTAGGCGACAAGTTTTATACTTTGAAAGAAAAAGAGAGTTTGGGGAAGAATTCCAATGTGGGTTTTGCTGAACTCTTTGCGAAAAGGCTGAATTTTTCGAAGGCGAAAAATGAGAGAACTGAGGGGCTAAGTGATGGTGGAGTGAAGGATGACGCTGTACATTTGAAGGACATTTTGAGGGAGTACAAGGGGAAGCTCTATGTGCCTGAGCAGATTTTCGGGACAGAGTTGCCGGAGGAAGAGCAATTTGAGAAGAGTTTGGAGGAATTGCCCAGAATGAGCTATGAGGAGTTTCTGAAAGTTGTGAAGAGTGATAAGGTTAAGTTGTTAATTTCCAAGGAAGTTGCAGGGACTTCTTATGGTGTCTGTGATTACATTGTTGATTTGAAGGAGATACCGGGTGAAAAGAGCTTGCATCGAACCAAATGGGCAATGAGGCTGGATGAGGGTGAAGCTCAAGCTCTTTTGGAGGATTACACAGGCCCGAGATATATGATTGAGGGGCATACTACGTCGTGGGTTGGAAAGGTACCACAGTACCCTCATCCGGTGGCATCTTCCATATCTAGCAGAATGATGGTGGAGCTTGGAATGGTAACAGCTGTAATGGCTGCTGCAGCAGTTTTTATTGGAGGATTCCTGGCTTCTGCTGTATTTGCTGTTACCAGCTTCGTTTTTGCTTCAACTGTATATGTTGTATTACCTATAGTCAAACCATTTTTGAGACTTTTTCTTGGTCTCGTCTTGGGTATTTTGGAGAGAGTATGGGACAACCTTGTTGACTTTTTCAGTGATGGAGGCATTTTCTCTAAATTTTCTGAATTTTACACATTTGGTGGTCTATCTGCCAGCATTGAGATGTTAAAACCGATAACAATTGTCCTTATGACCATGGTCATTCTTGTCCGTTTCACACTTTCAAGAAGACCTAAGAACTTCCGGAAGTGGGACCTGTGGCAAGGGATTGATTTTTCACGATCCAAAGCTGAAGCCCGTGTTGATGGGTCAACTGGAGTTAAGTTTGGTGATGTGGCAGGGATTGATGAAGCAGTAGAGGAACTCCTGGAGTTGGTGAGGTACTTGAAGAACCCAGAACTATTTGACAAAATGGGAATAAAACCACCGCATGGGGTTCTTTTAGAGGGTCCACCTGGATGTGGCAAGACCCTGGTTGCAAAGGCCATAGCTGGTGAGGCTGGTGTTCCGTTTTACCAAATGGCTGGATCTGAATTTGTTGAAGTCTTAGTTGGTGTTGGTTCAGCTCGTATTAGGGATCTATTCAAAAGAGCCAAGGTAAACAAACCATCTGTTATTTTCATCGATGAGATTGATGCGTTGGCTACGAGGCGTCAAGGAATTTTCAAGGAAACTAGCGACCACCTGTATAATGCAGCCACTCAAGAGAGAGAAACTACTCTGAACCAGCTCTTAATTGAGCTTGATGGATTTGATACCGGTAAAGGTGTTATATTCTTAGCTGCTACAAATCGCAGGGATTTGCTAGACCCTGCACTTCTTCGACCAGGTCGTTTTGACCGGAAGATTAAAATTCGTCCTCCTGCTGCGAAGGGGAGATTGGATATTTTGAAAATCCATGCAAGCAAAGTGAAAATGTCACCATCTGTTGATTTGTCCAGCTATGCACAGAACTTACCTGGATGGACGGGAGCAAAGTTGGCTCAGCTGGTCCAAGAGGCTGCACTTGTAGCAGTGAGGAAGGGGCATGATTCAATTTTTCAGTCAGACTTGGATGATGCTGTTGATAGACTCACTGTAGGACCAAAGCGGGTTGGAATAGAGTTGGGCCATCAGGGTCAATGTCGTCGAGCCACTACTGAAGTTGGAGTGGCAATGGCTTCTCATCTGCTTAGGCAATATGAGAATGCAGAAGTTGAACGATGTGATCGCATTTCAATCATCCCTCGTGGTCAGACATTGTCACAAGTTGTATTTCATCGACTTGATGATGAATCATACATGTTTGAACGCCGGCCACAATTACTGCATCGCCTTCAGGTTTTACTTGGTGGGAGGGCTGCTGAAGAGGTCATATATGGACGAGACACATCAAGGGCATCGGTTGACTACCTTGCTGATGCATCTTGGCTTGCTCGTAAAATTTTAACCATTTGGAATTTGGAGAATCCGATGGTCATACACGGAGAGCCACCACCCTGGAGGAAGAAACTTGAATTTGTAGGCCCTCGGCTGGACTTCGAGGGATCACTGTACCACGACTATGATCTGATTGAACCACCAGTGAACTTCAATTTAGATGATGATGTTGCAAAAAGGGCGGAAGAGCTGATACATAAAATGTATGACAAAACACTTTCTCTGCTTAAGAAGCATCACACGGCCTTGCTTAAAACTGTGAAGGTTCTTCTTGAACGCAAGGAAATTAGCGGTGAAGAAATTGATTTTATCTTGAACAAGTACCCTCCACAAACACCCTTAAAGCTTCTTTTAGAGGAGGAAAATCCTGGCAGCCTTAAGTTTGTCACACAAGAACAAGAACAGGAACAGGAACGTCAGTTAGAGTACGCCCTTCTATCACAATCCAAAGGGGAAACCCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009657 GO:0009658 GO:0009706 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010020 GO:0010154 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0019866 GO:0022414 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0042170 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

949

Amino Acids

106.69

Weight (kDa)

7.66

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	467 - 607	1.8e-39	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	630 - 673	9e-08	AAA+ lid domain
Peptidase_M41	PF01434	714 - 802	4.4e-12	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011834)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23940
fragaria_vesca	FvH4_5g04870 FvH4_5g04870
malus_domestica	MD06G1109500.v1.1 MD14G1130500.v1.1
prunus_persica	Prupe.5G122500_v2.0.a1
pyrus_communis	pycom06g10310
rosa_chinensis	RchiOBHm_Chr7g0194221
rosa_laevigata	RLG00000004209
rosa_multiflora	Rmu_co8268851.1_g000001 Rmu_sc0000901.1_g000001 Rmu_sc0009433.1_g000015
rosa_roxburghii	Rroxscaffold_3G00261490
rosa_rugosa	Rorug07G0014400
rosa_samantha	Rh7AG141800 Rh7BG142200 Rh7CG145900 Rh7DG143200
rosa_wichuraiana	Rw7G012120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	796
AccB1I	GGYRCC	1 cut(s)	796
AccB7I	CCANNNNNTGG	1 cut(s)	1394
AccIII	TCCGGA	1 cut(s)	1206
AciI	CCGC	6 cut(s)	113, 127, 1391, 2053, 2564, 2673
AclWI	GGATC	4 cut(s)	1239, 1498, 1548, 2497
AcoI	YGGCCR	2 cut(s)	167, 2258
AcuI	CTGAAG	4 cut(s)	1275, 2124, 2264, 2328
AcyI	GRCGYC	1 cut(s)	1615
AfaI	GTAC	9 cut(s)	263, 426, 452, 798, 806, 1346, 2498, 2705, 2810
AfiI	CCNNNNNNNGG	6 cut(s)	814, 1213, 1336, 1394, 1424, 2476
AflII	CTTAAG	2 cut(s)	2609, 2759
AflIII	ACRYGT	1 cut(s)	2244
AgeI	ACCGGT	2 cut(s)	132, 1721
AjuI	GAANNNNNNNTTGG	4 cut(s)	1497, 1529, 1826, 1858
Alw26I	GTCTC	3 cut(s)	985, 1010, 2322
AlwI	GGATC	4 cut(s)	1239, 1498, 1548, 2497
Ama87I	CYCGRG	1 cut(s)	751
Aor13HI	TCCGGA	1 cut(s)	1206
AoxI	GGCC	7 cut(s)	167, 748, 1446, 2071, 2258, 2467, 2625
AsiGI	ACCGGT	2 cut(s)	132, 1721
Asp700I	GAANNNNTTC	1 cut(s)	86
Asp718I	GGTACC	1 cut(s)	796
AspS9I	GGNCC	8 cut(s)	180, 749, 1216, 1412, 1954, 2045, 2071, 2468
AsuC2I	CCSGG	1 cut(s)	666
AsuHPI	GGTGA	9 cut(s)	133, 197, 680, 728, 1304, 1351, 1469, 1875, 2687
AsuII	TTCGAA	1 cut(s)	365
AvaI	CYCGRG	1 cut(s)	751
AvaII	GGWCC	5 cut(s)	180, 1216, 1412, 1954, 2045
BaeI	ACNNNNGTAYC	4 cut(s)	788, 788, 821, 821
BanI	GGYRCC	1 cut(s)	796
BarI	GAAGNNNNNNTAC	2 cut(s)	2559, 2591
BauI	CACGAG	1 cut(s)	2190
BbsI	GAAGAC	2 cut(s)	153, 1198
BceAI	ACGGC	2 cut(s)	154, 2640
BcnI	CCSGG	1 cut(s)	666
BcoDI	GTCTC	3 cut(s)	985, 1010, 2322
BfaI	CTAG	4 cut(s)	243, 834, 1638, 1769
BfmI	CTRYAG	3 cut(s)	882, 972, 2040
BfrI	CTTAAG	2 cut(s)	2609, 2759
BlpI	GCTNAGC	1 cut(s)	1947
Bme18I	GGWCC	5 cut(s)	180, 1216, 1412, 1954, 2045
BmeT110I	CYCGRG	1 cut(s)	751
BmgT120I	GGNCC	8 cut(s)	180, 749, 1216, 1412, 1954, 2045, 2071, 2468
BmiI	GGNNCC	3 cut(s)	798, 1217, 1413
BoxI	GACNNNNGTC	2 cut(s)	224, 1160
BpiI	GAAGAC	2 cut(s)	153, 1198
BpmI	CTGGAG	3 cut(s)	1299, 1352, 2464
Bpu10I	CCTNAGC	2 cut(s)	474, 2135
Bpu1102I	GCTNAGC	1 cut(s)	1947
Bpu14I	TTCGAA	1 cut(s)	365
BpuEI	CTTGAG	2 cut(s)	708, 1650
BpuMI	CCSGG	1 cut(s)	666
Bsa29I	ATCGAT	1 cut(s)	1587
BsaHI	GRCGYC	1 cut(s)	1615
BsaI	GGTCTC	1 cut(s)	1010
BsaJI	CCNNGG	9 cut(s)	204, 222, 257, 603, 1158, 1434, 1557, 2441, 2471
BsaWI	WCCGGW	6 cut(s)	132, 182, 814, 1206, 1721, 1803
BsaXI	ACNNNNNCTCC	2 cut(s)	129, 159
Bsc4I	CCNNNNNNNGG	6 cut(s)	814, 1213, 1336, 1394, 1424, 2476
Bse118I	RCCGGY	3 cut(s)	132, 1721, 2256
Bse1I	ACTGG	2 cut(s)	1282, 2525
Bse3DI	GCAATG	2 cut(s)	705, 2124
BseAI	TCCGGA	1 cut(s)	1206
BseCI	ATCGAT	1 cut(s)	1587
BseDI	CCNNGG	9 cut(s)	204, 222, 257, 603, 1158, 1434, 1557, 2441, 2471
BseGI	GGATG	8 cut(s)	5, 421, 715, 811, 1427, 1931, 2023, 2184
BseLI	CCNNNNNNNGG	6 cut(s)	814, 1213, 1336, 1394, 1424, 2476
BseMI	GCAATG	2 cut(s)	705, 2124
BseMII	CTCAG	4 cut(s)	89, 378, 465, 1961
BseNI	ACTGG	2 cut(s)	1282, 2525
BseRI	GAGGAG	3 cut(s)	137, 569, 2753
BseYI	CCCAGC	1 cut(s)	70
BsgI	GTGCAG	2 cut(s)	1761, 1950
BshFI	GGCC	7 cut(s)	169, 750, 1448, 2073, 2260, 2469, 2627
BshNI	GGYRCC	1 cut(s)	796
BshTI	ACCGGT	2 cut(s)	132, 1721
BshVI	ATCGAT	1 cut(s)	1587
BsiHKCI	CYCGRG	1 cut(s)	751
BsiSI	CCGG	9 cut(s)	133, 183, 500, 665, 815, 1207, 1722, 1804, 2257
BslFI	GGGAC	4 cut(s)	502, 631, 1049, 1229
BslI	CCNNNNNNNGG	6 cut(s)	814, 1213, 1336, 1394, 1424, 2476
BsmAI	GTCTC	3 cut(s)	985, 1010, 2322
BsmFI	GGGAC	4 cut(s)	502, 631, 1049, 1229
BsmI	GAATGC	1 cut(s)	2155
BsnI	GGCC	7 cut(s)	169, 750, 1448, 2073, 2260, 2469, 2627
Bso31I	GGTCTC	1 cut(s)	1010
BsoBI	CYCGRG	1 cut(s)	751
Bsp119I	TTCGAA	1 cut(s)	365
Bsp13I	TCCGGA	1 cut(s)	1206
Bsp1407I	TGTACA	1 cut(s)	424
Bsp143I	GATC	6 cut(s)	1244, 1490, 1540, 2170, 2489, 2509
Bsp1720I	GCTNAGC	1 cut(s)	1947
Bsp19I	CCATGG	1 cut(s)	1158
BspACI	CCGC	6 cut(s)	113, 127, 1391, 2053, 2564, 2673
BspANI	GGCC	7 cut(s)	169, 750, 1448, 2073, 2260, 2469, 2627
BspCNI	CTCAG	4 cut(s)	88, 379, 466, 1960
BspDI	ATCGAT	1 cut(s)	1587
BspEI	TCCGGA	1 cut(s)	1206
BspLI	GGNNCC	3 cut(s)	798, 1217, 1413
BspMAI	CTGCAG	1 cut(s)	886
BspPI	GGATC	4 cut(s)	1239, 1498, 1548, 2497
BspQI	GCTCTTC	2 cut(s)	501, 2562
BspT104I	TTCGAA	1 cut(s)	365
BspT107I	GGYRCC	1 cut(s)	796
BspTI	CTTAAG	2 cut(s)	2609, 2759
BspTNI	GGTCTC	1 cut(s)	1010
BsrDI	GCAATG	2 cut(s)	705, 2124
BsrFI	RCCGGY	3 cut(s)	132, 1721, 2256
BsrGI	TGTACA	1 cut(s)	424
BsrI	ACTGG	2 cut(s)	1282, 2525
BssAI	RCCGGY	3 cut(s)	132, 1721, 2256
BssECI	CCNNGG	9 cut(s)	204, 222, 257, 603, 1158, 1434, 1557, 2441, 2471
BssMI	GATC	6 cut(s)	1244, 1490, 1540, 2170, 2489, 2509
BssNI	GRCGYC	1 cut(s)	1615
BssSI	CACGAG	1 cut(s)	2190
BssT1I	CCWWGG	3 cut(s)	603, 1158, 1557
Bst2BI	CACGAG	1 cut(s)	2190
Bst4CI	ACNGT	5 cut(s)	804, 955, 2041, 2496, 2641
Bst6I	CTCTTC	5 cut(s)	501, 515, 569, 2304, 2562
BstACI	GRCGYC	1 cut(s)	1615
BstAFI	CTTAAG	2 cut(s)	2609, 2759
BstAUI	TGTACA	1 cut(s)	424
BstBI	TTCGAA	1 cut(s)	365
BstC8I	GCNNGC	5 cut(s)	680, 1117, 1867, 2258, 2376
BstDEI	CTNAG	9 cut(s)	75, 387, 395, 474, 1197, 1510, 1741, 1947, 2135
BstDSI	CCRYGG	1 cut(s)	1158
BstF5I	GGATG	8 cut(s)	5, 421, 715, 811, 1427, 1931, 2023, 2184
BstKTI	GATC	6 cut(s)	1247, 1493, 1543, 2173, 2492, 2512
BstMAI	GTCTC	3 cut(s)	985, 1010, 2322
BstMBI	GATC	6 cut(s)	1244, 1490, 1540, 2170, 2489, 2509
BstMWI	GCNNNNNNNGC	9 cut(s)	469, 875, 884, 923, 1460, 1943, 1973, 2561, 2614
BstNSI	RCATGY	1 cut(s)	2248
BstPAI	GACNNNNGTC	2 cut(s)	224, 1160
BstSFI	CTRYAG	3 cut(s)	882, 972, 2040
BstV2I	GAAGAC	2 cut(s)	153, 1198
BstX2I	RGATCY	2 cut(s)	1490, 1540
BstXI	CCANNNNNNTGG	3 cut(s)	265, 1487, 2442
BstYI	RGATCY	2 cut(s)	1490, 1540
Bsu15I	ATCGAT	1 cut(s)	1587
BsuRI	GGCC	7 cut(s)	169, 750, 1448, 2073, 2260, 2469, 2627
BsuTUI	ATCGAT	1 cut(s)	1587
BtgI	CCRYGG	1 cut(s)	1158
BtgZI	GCGATG	1 cut(s)	2258
BtsCI	GGATG	8 cut(s)	5, 421, 715, 811, 1427, 1931, 2023, 2184
BtsI	GCAGTG	1 cut(s)	1983
BtsIMutI	CAGTG	5 cut(s)	1063, 1983, 2037, 2492, 2532
Cac8I	GCNNGC	5 cut(s)	680, 1117, 1867, 2258, 2376
Cfr10I	RCCGGY	3 cut(s)	132, 1721, 2256
Cfr13I	GGNCC	8 cut(s)	180, 749, 1216, 1412, 1954, 2045, 2071, 2468
ClaI	ATCGAT	1 cut(s)	1587
CseI	GACGC	2 cut(s)	428, 1604
CsiI	ACCWGGT	1 cut(s)	1789
Csp6I	GTAC	9 cut(s)	262, 425, 451, 797, 805, 1345, 2497, 2704, 2809
CspAI	ACCGGT	2 cut(s)	132, 1721
CviAII	CATG	5 cut(s)	1159, 1394, 1862, 1991, 2245
CviQI	GTAC	9 cut(s)	262, 425, 451, 797, 805, 1345, 2497, 2704, 2809
DdeI	CTNAG	9 cut(s)	75, 387, 395, 474, 1197, 1510, 1741, 1947, 2135
DpnI	GATC	6 cut(s)	1246, 1492, 1542, 2172, 2491, 2511
DpnII	GATC	6 cut(s)	1244, 1490, 1540, 2170, 2489, 2509
EaeI	YGGCCR	2 cut(s)	167, 2258
Eam1104I	CTCTTC	5 cut(s)	501, 515, 569, 2304, 2562
EarI	CTCTTC	5 cut(s)	501, 515, 569, 2304, 2562
EciI	GGCGGA	2 cut(s)	116, 2579
Eco130I	CCWWGG	3 cut(s)	603, 1158, 1557
Eco31I	GGTCTC	1 cut(s)	1010
Eco47I	GGWCC	5 cut(s)	180, 1216, 1412, 1954, 2045
Eco57I	CTGAAG	4 cut(s)	1275, 2124, 2264, 2328
Eco88I	CYCGRG	1 cut(s)	751
EcoO109I	RGGNCCY	2 cut(s)	1216, 2468
EcoRI	GAATTC	1 cut(s)	316
EcoT14I	CCWWGG	3 cut(s)	603, 1158, 1557
EcoT22I	ATGCAT	1 cut(s)	2368
ErhI	CCWWGG	3 cut(s)	603, 1158, 1557
FaeI	CATG	5 cut(s)	1162, 1397, 1865, 1994, 2248
FaqI	GGGAC	4 cut(s)	502, 631, 1049, 1229
FatI	CATG	5 cut(s)	1158, 1393, 1861, 1990, 2244
FauI	CCCGC	1 cut(s)	2046
FokI	GGATG	7 cut(s)	428, 722, 798, 1434, 1938, 2030, 2171
FspBI	CTAG	4 cut(s)	243, 834, 1638, 1769
GsaI	CCCAGC	1 cut(s)	74
GsuI	CTGGAG	3 cut(s)	1299, 1352, 2464
HaeIII	GGCC	7 cut(s)	169, 750, 1448, 2073, 2260, 2469, 2627
HapII	CCGG	9 cut(s)	133, 183, 500, 665, 815, 1207, 1722, 1804, 2257
HgaI	GACGC	2 cut(s)	428, 1604
Hin1I	GRCGYC	1 cut(s)	1615
Hin1II	CATG	5 cut(s)	1162, 1397, 1865, 1994, 2248
HincII	GTYRAC	3 cut(s)	1048, 1275, 2350
HindII	GTYRAC	3 cut(s)	1048, 1275, 2350
HindIII	AAGCTT	1 cut(s)	2726
HinfI	GANTC	6 cut(s)	97, 903, 1994, 2034, 2237, 2410
HpaII	CCGG	9 cut(s)	133, 183, 500, 665, 815, 1207, 1722, 1804, 2257
HphI	GGTGA	9 cut(s)	133, 197, 680, 728, 1304, 1351, 1469, 1875, 2687
Hpy166II	GTNNAC	6 cut(s)	1048, 1275, 1415, 1564, 2350, 2530
Hpy188I	TCNGA	9 cut(s)	102, 564, 1087, 1495, 1686, 2011, 2199, 2415, 2514
Hpy188III	TCNNGA	8 cut(s)	229, 487, 1188, 1207, 1242, 1667, 2654, 2695
Hpy8I	GTNNAC	6 cut(s)	1048, 1275, 1415, 1564, 2350, 2530
Hpy99I	CGWCG	3 cut(s)	175, 784, 2094
HpyCH4III	ACNGT	5 cut(s)	804, 955, 2041, 2496, 2641
HpyCH4IV	ACGT	2 cut(s)	779, 2797
HpyF10VI	GCNNNNNNNGC	9 cut(s)	469, 875, 884, 923, 1460, 1943, 1973, 2561, 2614
HpyF3I	CTNAG	9 cut(s)	75, 387, 395, 474, 1197, 1510, 1741, 1947, 2135
HpySE526I	ACGT	2 cut(s)	779, 2797
Hsp92I	GRCGYC	1 cut(s)	1615
Hsp92II	CATG	5 cut(s)	1162, 1397, 1865, 1994, 2248
Kpn2I	TCCGGA	1 cut(s)	1206
KpnI	GGTACC	1 cut(s)	800
KroI	GCCGGC	1 cut(s)	2256
KroNI	GCCGGC	1 cut(s)	2258
Kzo9I	GATC	6 cut(s)	1244, 1490, 1540, 2170, 2489, 2509
LguI	GCTCTTC	2 cut(s)	501, 2562
LmnI	GCTCC	2 cut(s)	850, 1934
MabI	ACCWGGT	1 cut(s)	1789
MaeI	CTAG	4 cut(s)	243, 834, 1638, 1769
MaeII	ACGT	2 cut(s)	779, 2797
MaeIII	GTNAC	8 cut(s)	135, 185, 217, 862, 928, 1881, 2205, 2767
MalI	GATC	6 cut(s)	1246, 1492, 1542, 2172, 2491, 2511
MboI	GATC	6 cut(s)	1244, 1490, 1540, 2170, 2489, 2509
MfeI	CAATTG	1 cut(s)	1143
MflI	RGATCY	2 cut(s)	1490, 1540
MlyI	GAGTC	1 cut(s)	2028
MmeI	TCCRAC	3 cut(s)	769, 2038, 2089
Mph1103I	ATGCAT	1 cut(s)	2368
MroI	TCCGGA	1 cut(s)	1206
MroNI	GCCGGC	1 cut(s)	2256
MroXI	GAANNNNTTC	1 cut(s)	86
MslI	CAYNNNNRTG	3 cut(s)	1124, 1420, 2505
MspA1I	CMGCKG	3 cut(s)	118, 869, 1951
MspCI	CTTAAG	2 cut(s)	2609, 2759
MspI	CCGG	9 cut(s)	133, 183, 500, 665, 815, 1207, 1722, 1804, 2257
MunI	CAATTG	1 cut(s)	1143
Mva1269I	GAATGC	1 cut(s)	2155
MwoI	GCNNNNNNNGC	9 cut(s)	469, 875, 884, 923, 1460, 1943, 1973, 2561, 2614
NaeI	GCCGGC	1 cut(s)	2258
NciI	CCSGG	1 cut(s)	666
NcoI	CCATGG	1 cut(s)	1158
NdeII	GATC	6 cut(s)	1244, 1490, 1540, 2170, 2489, 2509
NgoMIV	GCCGGC	1 cut(s)	2256
NlaIII	CATG	5 cut(s)	1162, 1397, 1865, 1994, 2248
NlaIV	GGNNCC	3 cut(s)	798, 1217, 1413
NmeAIII	GCCGAG	1 cut(s)	2452
NmuCI	GTSAC	6 cut(s)	135, 185, 217, 1881, 2205, 2767
NsiI	ATGCAT	1 cut(s)	2368
NspI	RCATGY	1 cut(s)	2248
NspV	TTCGAA	1 cut(s)	365
PciI	ACATGT	1 cut(s)	2244
PciSI	GCTCTTC	2 cut(s)	501, 2562
PcsI	WCGNNNNNNNCGW	1 cut(s)	1792
PctI	GAATGC	1 cut(s)	2155
PdiI	GCCGGC	1 cut(s)	2258
PdmI	GAANNNNTTC	1 cut(s)	86
PfeI	GAWTC	5 cut(s)	97, 903, 1994, 2237, 2410
PflFI	GACNNNGTC	2 cut(s)	1791, 2203
PflMI	CCANNNNNTGG	1 cut(s)	1394
PfoI	TCCNGGA	1 cut(s)	1329
PinAI	ACCGGT	2 cut(s)	132, 1721
PleI	GAGTC	1 cut(s)	2028
PpsI	GAGTC	1 cut(s)	2028
PpuMI	RGGWCCY	1 cut(s)	1216
PscI	ACATGT	1 cut(s)	2244
PshAI	GACNNNNGTC	2 cut(s)	224, 1160
Psp5II	RGGWCCY	1 cut(s)	1216
PspFI	CCCAGC	1 cut(s)	70
PspN4I	GGNNCC	3 cut(s)	798, 1217, 1413
PspPI	GGNCC	8 cut(s)	180, 749, 1216, 1412, 1954, 2045, 2071, 2468
PspPPI	RGGWCCY	1 cut(s)	1216
PstI	CTGCAG	1 cut(s)	886
PsuI	RGATCY	2 cut(s)	1490, 1540
PsyI	GACNNNGTC	2 cut(s)	1791, 2203
PvuII	CAGCTG	3 cut(s)	118, 869, 1951
RsaI	GTAC	9 cut(s)	263, 426, 452, 798, 806, 1346, 2498, 2705, 2810
RsaNI	GTAC	9 cut(s)	262, 425, 451, 797, 805, 1345, 2497, 2704, 2809
RseI	CAYNNNNRTG	3 cut(s)	1124, 1420, 2505
SapI	GCTCTTC	2 cut(s)	501, 2562
Sau3AI	GATC	6 cut(s)	1244, 1490, 1540, 2170, 2489, 2509
Sau96I	GGNCC	8 cut(s)	180, 749, 1216, 1412, 1954, 2045, 2071, 2468
SchI	GAGTC	1 cut(s)	2028
SexAI	ACCWGGT	1 cut(s)	1789
SfcI	CTRYAG	3 cut(s)	882, 972, 2040
SfuI	TTCGAA	1 cut(s)	365
SinI	GGWCC	5 cut(s)	180, 1216, 1412, 1954, 2045
SmiMI	CAYNNNNRTG	3 cut(s)	1124, 1420, 2505
SmlI	CTYRAG	4 cut(s)	723, 1665, 2609, 2759
SmoI	CTYRAG	4 cut(s)	723, 1665, 2609, 2759
SsiI	CCGC	6 cut(s)	113, 127, 1391, 2053, 2564, 2673
SspMI	CTAG	4 cut(s)	243, 834, 1638, 1769
StyI	CCWWGG	3 cut(s)	603, 1158, 1557
TaaI	ACNGT	5 cut(s)	804, 955, 2041, 2496, 2641
TaiI	ACGT	2 cut(s)	782, 2800
TaqI	TCGA	8 cut(s)	80, 365, 685, 1587, 1786, 2092, 2224, 2484
TatI	WGTACW	2 cut(s)	424, 450
TfiI	GAWTC	5 cut(s)	97, 903, 1994, 2237, 2410
TscAI	CASTG	5 cut(s)	1063, 1983, 2044, 2499, 2532
TseFI	GTSAC	6 cut(s)	135, 185, 217, 1881, 2205, 2767
Tsp45I	GTSAC	6 cut(s)	135, 185, 217, 1881, 2205, 2767
TspDTI	ATGAA	4 cut(s)	1326, 1573, 2210, 2250
TspGWI	ACGGA	3 cut(s)	1163, 1461, 2442
TspRI	CASTG	5 cut(s)	1063, 1983, 2044, 2499, 2532
Tth111I	GACNNNGTC	2 cut(s)	1791, 2203
Van91I	CCANNNNNTGG	1 cut(s)	1394
Vha464I	CTTAAG	2 cut(s)	2609, 2759
VpaK11BI	GGWCC	5 cut(s)	180, 1216, 1412, 1954, 2045
XceI	RCATGY	1 cut(s)	2248
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2401
XmnI	GAANNNNTTC	1 cut(s)	86
XspI	CTAG	4 cut(s)	243, 834, 1638, 1769
Zsp2I	ATGCAT	1 cut(s)	2368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.