Rmu_sc0009433.1_g000015

ATP-dependent zinc metalloprotease

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009433.1
Physical Location & Seq
Reverse (-)
58442 .. 71724
13283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009433.1_g000015.1.cds

Sequence Viewer

Length: 6264 bp
atggtatccattgacattctaaactcacccaggctttacattcccaaaccccacagccacttcaaatctctagctcactcaaagcacctcaatttgataagaagaattcaaaagcagccagctttccctcgccggtcactcacggtcctctgctcacggtccggtgacgctagcaaagcccctgaggatgattttgtgaccagggtcttgaaggagaacccaagccagattgaacccaggtacttgattggtgaaaagttttatactttgaaggaaaaagagagtttgagcaagaaatccaatgtgggttttgctgaactcttggcaaagaggttgaatttcaacaaggcagagctgaagaaacagagcggtgaggctcaaagtggtagtggagtgaagaagaatgaggaggctgtgtttttgaatgatattttgagggagtacaaggggaagctctatgtgcctgagcaaattttcggaaccgagttgccggaggaagatgaatttgagaagagtttggaggaattgcccaagatgagctttgaggattttcagaaagctatgaagaatgataaggttgacttgttgacttctaaggaagttaagggtgcttcttatgggtccagggatttcattgttgatttgaaggagatcccaggcgagaagagcttgcatagaaccaaatgggcaatgaggctggatgagggtgaagctcttgctcttttggaggattatacgggtccaagatatgtgatagaaagacataccacgtcttccgttggaagcttaccacagtaccctcatccggtggcgtcttccatatcgagcagaatgatggtagagcttggagtggtaacagcattaatggttgctgctgcagttgttgttggaggattcctggcgtctgcagtatttgctgttaccagtttcgtgtttgtgtcaactgtatatgttgtgtggcctatagtcaagccgtttatcaggcttttctatttatcaggatggacgggagcaaagttggcacagctggtccaagaggctgcacttgttgcagtaaggaaggggcatgattcaatttatcggtcagacttggatgatgcagttgatagacttacagtaggaccaaaacgggttggaatcgatttgggtcatcagggacaatgccgtcgagctactactgaagttggagttgcattgacttctcacctgcttaggcaatatgagaatgcagaagttgagtcctgtgatcgcatttcaatcattcctcgtggccagacactgtcgcaagttgtatttgatcgtcttgatgatgaatcatacatgtttgagcgtcggccacaattgttgcatcgccttcaggttttacttggagggagggctgctgaagaggtcatatatggacgggacacatcaagggcatcagttgactaccttgctgatgcatcttggcttgctcgtaaaatcttaactgtttggaatttggagaatccaatggtcatacatggagagccaccaccttggaggaagaaacccaagtttgtcggccctcgactggactttgagggttcattgtacgatgactatggcctgattgaaccaccagtgaacttcaatttagatgatcaggttgcacaaaggactgaagagctggtagagagcatgtatgaaaaaacgctttctttgcttaagcggcatcatgcagccttgcttaaaaccgtgaaagttcttcttgaacacaaggaaatcagcggtgaagaaattgattttatactgaacaagtatcctccccaaacacccgtgaagcttctattagaagaagaaaaccctggtagcctggacttcatgaaacaggaagagaaacgatggacgggagcaaagttggcacagctggtccaagaggctgcacttgttgcagtaaggaaggggcatgattcaatttatcggtcagacttggatgatgcagttgatagacttacagtaggaccaaaacgggttggaatcgatttgggtcatcagggacaatgccgtcgagctactactgaagttggagttgcattgacttctcacctgcttaggcaatatgagaatgcagaagttgagtcctgtgatcgcatttcaatcattcctcgtggccagacactgtcgcaagttgtatttgatcgtcttgatgatgaatcatacatgtttgagcgtcggccacaattgttgcatcgccttcaggttttacttggagggagggctgctgaagaggtcatatatggacgggacacatcaagggcatcagttgactaccttgctgatgcatcttggcttgctcgtaaaatcttaactgtttggaatttggagaatccaatggtcatacatggagagccaccaccttggaggaagaaacccaagtttgtcggccctcgactggactttgagggttcattgtacgatgactatggcctgattgaaccaccagtgaacttcaatttagatgatcaggttgcacaaaggactgaagagctggtagagagcatgtatgaaaaaacgctttctttgcttaagcggcatcatgcagccttgcttaaaaccgtgaaagttcttcttgaacacaaggaaatcagcggtgaagaaattgattttatactgaacaagtatcctccccaaacacccgtgaagcttctattagaagaagaaaaccctggtagcctggacttcatgaaacaggaagcgaaacgatggacgggagcaaagttggcacagctggtccaagaggctgcacttgttgcagtaaggaaggggcatgattcaatttatcggtcagacttggatgatgcagttgatagacttacagtaggaccaaaacgggttggaatcgatttgggtcatcagggacaatgccgtcgagctactactgaagttggagttgcattgacttctcacctgcttaggcaatatgagaatgcagaagttgagtcctgtgatcgcatttcaatcattcctcgtggccagacactgtcgcaagttgtatttgatcgtcttgatgatgaatcatacatgtttgagcgtcggccacaattgttgcatcgccttcaggttttacttggagggagggctgctgaagaggtcatatatggacgggacacatcaagggcatcagttgactaccttgctgatgcatcttggcttgctcgtaaaatcttaactgtttggaatttggagaatccaatggtcatacatggagagccaccaccttggaggaagaaacccaagtttgtcggccctcgactggactttgagggttcattgtacgatgactatggcctgattgaaccaccagtgaacttcaatttagatgatcaggttgcacaaaggactgaagagctggtagagagcatgtatgaaaaaacgctttctttgcttaagcggcatcatgcagccttgcttaaaaccgtgaaagttcttcttgaacacaaggaaatcagcggtgaagaaattgattttatactgaacaagtatcctccccaaacacccgtgaagcttctattagaagaagaaaaccctggtagcctggacttcatgaaacaggaagcgaaacgtgagcaatatgagaatgcagaagttgagtcctgtgatcgcatttcaatcattcctcgtggccaggttttacttggagggagggctgctgaagaggtcatatatggacgggacacatcaagggcatcagttgactaccttgctgatgcatcttggcttgctcgtaaaatcttaactgtttggaatttggagaatccaatggtcatacatggagagccaccaccttggaggaagaaacccaagtttgtcggccctcgactggactttgagggttcattgtacgatgactatggcctgattgaaccaccagtgaacttcaatttagatgatcaggttgcacaaaggactgaagagctggtagagagcatgtatgaaaaaacgctttctttgcttaagcggcatcatgctgccttgcttaaaaccgtgaaagttcttcttgaacacaaggaaatcagcggtgaagaaattgattttatactgaacaagtatcctccccaaacacccgtgaagcttctattagaagaagaaaaccctggtagcctggacttcatgaaacaggaagcgaaacaacatgagagagagatgaagaagcctcgccacggttccttacatatcaaaatccaaagcaacaacaactctgacaccaaggacagaggagacaaaaacaacaagagcagcaaccactacaacaacaataaagggaagagcttctatgagctctctctgtctttgatcttctctctctggtgtcttgttgtcctgttctactctaagcttggtcttggccatggcaatggagggaatatttcaggtccgagtaacagaactacacattgtcctggtgttcacaatggtaagcatgcatattcatttgtggcaaatggaggcgaaaataagacaaatgggatgcttttagattataattcgtctgaaagttgtaatggtactgctgttggtcacaacttgccagtttcgaagtactcactgccggagacaaatggattagaaaagatagtttggagagttttgggttatagcagtttggtctgtgaactcaaccaagaacaagaacagcatagcagaaactgtccaggacaatgtgtgaatagaacttctcactctacctatctcaatcttgatgaatttcgaaacgtaacaaagcaagagaaagaccgtgtaatgcctagtcagcttgttaacattacccatgggttcgaacctgatggaacggagtacaactatgcctccgaatccaagggggcaaaggtggtgtctcacaacaaggaagcaaaaggagcaagtaacatattaggcaaggatcacgataaatacctcagaaacccttgttccgtagagggaaagtttgttataattgagctcgcagaggagactctggtagatgctgtcaaaattgcaaattttgagcactactcttctaatttcaaggaatttaagttgtctggcagtttaagctatccaacagaagcatggtcaccgctgggtagctttgttgctgctaatagtaagcatgctcaaacctttaagcttcctgaacccaaatgggtgagatacctaaagttggatttagtaagccactacggagcagagttttactgcacacttagtgttctagaggtgtatggtattaatgcaatcgaaagaatgcttgaagatctcattgtggagtctgcagaatctgcaaccaacaagttgccagagcctaatgcaactgtaatatcttctacaaaagaagaggttggtccaactgaccggaaaataagcagcgatagtcaaaatgggattggtgcagctgtagggattcaaagcactgaagaagcgcaacgagccaattcagatgcttccaagaattcagtaactactaaccagattcctgatccggttatgacagttcggcagcagccaaatggaagaattcctggtgattctgttctgaagattttgatgcagaaggtgcggtcacttgagctgaacttgtccgtgctggaggagtttatcaaagaattgaacagaagacaaggcgatgttttgccagagcttggaaaagagatattaagaatatcattgctgttggagcaaagcaaaatggagattaaagatctcatgcaatggaaggaaactatggaaaaacaaatgagtgaccttgaactatggaaagctgctgtctcgtctcaattgaatgcattggtcagggaaaataacttgttgagattagatttcgaaaaggttgtaaatgatcaagccagtctcgaaagcaaagagcttgctattttagcagtttttccagacgtcagtttctcttctcgtccggcggctcctcctgcgttggcgtgggcttctagcctcgtctgcgtgtggcgtgggcttctggcctcgtctgcgtgtggcgagggcagccggacgggtcttttcccttcttgggatcgtgggttcctcatggcgggctttggcttcgatttcttctcggatggtggtggtcgacacatcgtgctagttcacaagaaaacaggctccggcagccggcgattggttcaatctgatttcgcaggtccacatccatgcaccgctgaaagcctccaatcgtggtggtgtggtttgcgatttggagggcgggattggagatttgcaggatcttcaatgggttggagagttgggatggaatag

Protein Analysis

2087

Amino Acids

235.19

Weight (kDa)

6.09

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Orthologous Genes (Group: OG0011834)

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4503, 4952
AarI CACCTGC 3 cut(s) 1215, 2076, 2937
AasI GACNNNNNNGTC 3 cut(s) 1164, 2025, 2886
AatII GACGTC 1 cut(s) 5892
Acc36I ACCTGC 4 cut(s) 1215, 2076, 2937, 6137
AccB7I CCANNNNNTGG 1 cut(s) 5639
AccBSI CCGCTC 1 cut(s) 369
AccI GTMKAC 1 cut(s) 6079
AclWI GGATC 5 cut(s) 646, 4908, 5471, 6030, 6238
AcoI YGGCCR 8 cut(s) 1270, 1334, 2131, 2195, 2992, 3056, 3643, 4366
AcyI GRCGYC 3 cut(s) 812, 902, 5889
AdeI CACNNNGTG 2 cut(s) 5207, 6088
AflII CTTAAG 4 cut(s) 1685, 2546, 3407, 3974
AflIII ACRYGT 3 cut(s) 1320, 2181, 3042
AjiI CACGTC 1 cut(s) 771
AloI GAACNNNNNNTCC 2 cut(s) 6014, 6046
Alw21I GWGCWC 3 cut(s) 4302, 4962, 5010
Alw26I GTCTC 7 cut(s) 4233, 4568, 4860, 4964, 5770, 5775, 5852
AlwI GGATC 5 cut(s) 646, 4908, 5471, 6030, 6238
AlwNI CAGNNNCTG 5 cut(s) 1279, 2140, 3001, 4668, 5279
ArsI GACNNNNNNTTYG 2 cut(s) 4838, 4870
AseI ATTAAT 2 cut(s) 863, 5229
Asp700I GAANNNNTTC 1 cut(s) 4289
AspLEI GCGC 1 cut(s) 5422
AsuII TTCGAA 4 cut(s) 4556, 4729, 4797, 5820
AsuNHI GCTAGC 1 cut(s) 170
AxyI CCTNAGG 1 cut(s) 183
BaeI ACNNNNGTAYC 4 cut(s) 779, 812, 4518, 4551
BalI TGGCCA 5 cut(s) 1272, 2133, 2994, 3645, 4368
BanII GRGCYC 2 cut(s) 4302, 4962
BauI CACGAG 4 cut(s) 1266, 2127, 2988, 3639
BbsI GAAGAC 3 cut(s) 765, 807, 5620
Bbv12I GWGCWC 3 cut(s) 4302, 4962, 5010
BccI CCATC 7 cut(s) 829, 996, 1857, 2718, 4799, 6062, 6250
BceAI ACGGC 4 cut(s) 958, 1149, 2010, 2871
BciVI GTATCC 5 cut(s) 16, 1791, 2652, 3513, 4080
BclI TGATCA 5 cut(s) 1621, 2482, 3343, 3910, 5836
BcoDI GTCTC 7 cut(s) 4233, 4568, 4860, 4964, 5770, 5775, 5852
BfaI CTAG 6 cut(s) 71, 171, 4767, 5213, 5940, 6092
BfmI CTRYAG 5 cut(s) 876, 906, 963, 5271, 5394
BfrI CTTAAG 4 cut(s) 1685, 2546, 3407, 3974
BfuAI ACCTGC 4 cut(s) 1215, 2076, 2937, 6137
BfuI GTATCC 5 cut(s) 16, 1791, 2652, 3513, 4080
BglII AGATCT 2 cut(s) 5254, 5698
BmcAI AGTACT 1 cut(s) 4562
BmgBI CACGTC 1 cut(s) 771
BmiI GGNNCC 7 cut(s) 481, 622, 741, 4186, 5916, 6032, 6112
BmtI GCTAGC 1 cut(s) 174
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 3 cut(s) 765, 807, 5620
BplI GAGNNNNNCTC 2 cut(s) 4997, 5029
BpmI CTGGAG 1 cut(s) 5606
Bpu10I CCTNAGC 4 cut(s) 465, 1211, 2072, 2933
Bpu14I TTCGAA 4 cut(s) 4556, 4729, 4797, 5820
BpuEI CTTGAG 1 cut(s) 5585
Bsa29I ATCGAT 3 cut(s) 1140, 2001, 2862
BsaHI GRCGYC 3 cut(s) 812, 902, 5889
BsaWI WCCGGW 4 cut(s) 161, 805, 5352, 5479
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 4811, 4841
Bse118I RCCGGY 2 cut(s) 132, 6120
Bse21I CCTNAGG 1 cut(s) 183
Bse3DI GCAATG 4 cut(s) 696, 4381, 5663, 5714
BseCI ATCGAT 3 cut(s) 1140, 2001, 2862
BseMI GCAATG 4 cut(s) 696, 4381, 5663, 5714
BseMII CTCAG 3 cut(s) 174, 456, 4930
BseRI GAGGAG 5 cut(s) 422, 4251, 4982, 5603, 5907
BseYI CCCAGC 1 cut(s) 5080
BsgI GTGCAG 5 cut(s) 1026, 1887, 2748, 5182, 5409
BshVI ATCGAT 3 cut(s) 1140, 2001, 2862
BsiHKAI GWGCWC 3 cut(s) 4302, 4962, 5010
BslFI GGGAC 7 cut(s) 1170, 1418, 2031, 2279, 2892, 3140, 3707
BsmAI GTCTC 7 cut(s) 4233, 4568, 4860, 4964, 5770, 5775, 5852
BsmBI CGTCTC 1 cut(s) 5775
BsmFI GGGAC 7 cut(s) 1170, 1418, 2031, 2279, 2892, 3140, 3707
BsmI GAATGC 6 cut(s) 1231, 2092, 2953, 3604, 5250, 5785
Bsp119I TTCGAA 4 cut(s) 4556, 4729, 4797, 5820
Bsp1286I GDGCHC 3 cut(s) 4302, 4962, 5010
Bsp19I CCATGG 2 cut(s) 4369, 4789
BspCNI CTCAG 3 cut(s) 175, 457, 4929
BspDI ATCGAT 3 cut(s) 1140, 2001, 2862
BspHI TCATGA 4 cut(s) 1842, 2703, 3564, 4131
BspLI GGNNCC 7 cut(s) 481, 622, 741, 4186, 5916, 6032, 6112
BspMAI CTGCAG 3 cut(s) 880, 910, 5275
BspMI ACCTGC 4 cut(s) 1215, 2076, 2937, 6137
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 5 cut(s) 646, 4908, 5471, 6030, 6238
BspQI GCTCTTC 6 cut(s) 659, 1638, 2499, 3360, 3927, 4280
BspT104I TTCGAA 4 cut(s) 4556, 4729, 4797, 5820
BspTI CTTAAG 4 cut(s) 1685, 2546, 3407, 3974
BsrBI CCGCTC 1 cut(s) 369
BsrDI GCAATG 4 cut(s) 696, 4381, 5663, 5714
BsrFI RCCGGY 2 cut(s) 132, 6120
BssAI RCCGGY 2 cut(s) 132, 6120
BssNI GRCGYC 3 cut(s) 812, 902, 5889
BssSI CACGAG 4 cut(s) 1266, 2127, 2988, 3639
BssT1I CCWWGG 8 cut(s) 1517, 2378, 3239, 3806, 4227, 4369, 4789, 4836
Bst2BI CACGAG 4 cut(s) 1266, 2127, 2988, 3639
BstACI GRCGYC 3 cut(s) 812, 902, 5889
BstAFI CTTAAG 4 cut(s) 1685, 2546, 3407, 3974
BstAPI GCANNNNNTGC 6 cut(s) 914, 1049, 1910, 2771, 5279, 5554
BstBI TTCGAA 4 cut(s) 4556, 4729, 4797, 5820
BstDEI CTNAG 9 cut(s) 183, 465, 594, 1211, 2072, 2933, 4353, 4916, 5204
BstDSI CCRYGG 3 cut(s) 4180, 4369, 4789
BstEII GGTNACC 1 cut(s) 5073
BstHHI GCGC 1 cut(s) 5422
BstMAI GTCTC 7 cut(s) 4233, 4568, 4860, 4964, 5770, 5775, 5852
BstNSI RCATGY 9 cut(s) 1324, 1663, 2185, 2524, 3046, 3385, 3952, 4445, 5114
BstPAI GACNNNNGTC 1 cut(s) 203
BstPI GGTNACC 1 cut(s) 5073
BstSFI CTRYAG 5 cut(s) 876, 906, 963, 5271, 5394
BstV2I GAAGAC 3 cut(s) 765, 807, 5620
BstX2I RGATCY 4 cut(s) 651, 5254, 5698, 6230
BstXI CCANNNNNNTGG 5 cut(s) 1518, 2379, 3240, 3807, 4376
BstYI RGATCY 4 cut(s) 651, 5254, 5698, 6230
Bsu15I ATCGAT 3 cut(s) 1140, 2001, 2862
Bsu36I CCTNAGG 1 cut(s) 183
BsuI GTATCC 5 cut(s) 16, 1791, 2652, 3513, 4080
BsuTUI ATCGAT 3 cut(s) 1140, 2001, 2862
BtgI CCRYGG 3 cut(s) 4180, 4369, 4789
BtgZI GCGATG 4 cut(s) 1334, 2195, 3056, 5637
BtrI CACGTC 1 cut(s) 771
BtsI GCAGTG 1 cut(s) 4565
BtsIMutI CAGTG 9 cut(s) 1277, 1608, 2138, 2469, 2999, 3330, 3897, 4565, 5409
BveI ACCTGC 4 cut(s) 1215, 2076, 2937, 6137
CaiI CAGNNNCTG 5 cut(s) 1279, 2140, 3001, 4668, 5279
CciI TCATGA 4 cut(s) 1842, 2703, 3564, 4131
CfoI GCGC 1 cut(s) 5422
Cfr10I RCCGGY 2 cut(s) 132, 6120
ClaI ATCGAT 3 cut(s) 1140, 2001, 2862
CpoI CGGWCCG 1 cut(s) 159
CseI GACGC 6 cut(s) 176, 801, 891, 1319, 2180, 3041
CspCI CAANNNNNGTGG 8 cut(s) 1326, 1361, 2187, 2222, 3048, 3083, 6167, 6202
CspI CGGWCCG 1 cut(s) 159
DdeI CTNAG 9 cut(s) 183, 465, 594, 1211, 2072, 2933, 4353, 4916, 5204
DraIII CACNNNGTG 2 cut(s) 5207, 6088
DrdI GACNNNNNNGTC 3 cut(s) 1164, 2025, 2886
DseDI GACNNNNNNGTC 3 cut(s) 1164, 2025, 2886
EaeI YGGCCR 8 cut(s) 1270, 1334, 2131, 2195, 2992, 3056, 3643, 4366
Ecl136II GAGCTC 2 cut(s) 4300, 4960
Eco130I CCWWGG 8 cut(s) 1517, 2378, 3239, 3806, 4227, 4369, 4789, 4836
Eco24I GRGCYC 2 cut(s) 4302, 4962
Eco53kI GAGCTC 2 cut(s) 4300, 4960
Eco81I CCTNAGG 1 cut(s) 183
Eco91I GGTNACC 1 cut(s) 5073
EcoICRI GAGCTC 2 cut(s) 4300, 4960
EcoO65I GGTNACC 1 cut(s) 5073
EcoRI GAATTC 3 cut(s) 105, 5449, 5514
EcoT14I CCWWGG 8 cut(s) 1517, 2378, 3239, 3806, 4227, 4369, 4789, 4836
EcoT22I ATGCAT 6 cut(s) 1444, 2305, 3166, 3733, 4447, 5785
EcoT38I GRGCYC 2 cut(s) 4302, 4962
ErhI CCWWGG 8 cut(s) 1517, 2378, 3239, 3806, 4227, 4369, 4789, 4836
Esp3I CGTCTC 1 cut(s) 5775
FaqI GGGAC 7 cut(s) 1170, 1418, 2031, 2279, 2892, 3140, 3707
FauI CCCGC 2 cut(s) 6034, 6204
FbaI TGATCA 5 cut(s) 1621, 2482, 3343, 3910, 5836
FblI GTMKAC 1 cut(s) 6079
FriOI GRGCYC 2 cut(s) 4302, 4962
FspBI CTAG 6 cut(s) 71, 171, 4767, 5213, 5940, 6092
GlaI GCGC 1 cut(s) 5421
GsaI CCCAGC 1 cut(s) 5084
GsuI CTGGAG 1 cut(s) 5606
HgaI GACGC 6 cut(s) 176, 801, 891, 1319, 2180, 3041
HhaI GCGC 1 cut(s) 5422
Hin1I GRCGYC 3 cut(s) 812, 902, 5889
Hin6I GCGC 1 cut(s) 5420
HinP1I GCGC 1 cut(s) 5420
HincII GTYRAC 9 cut(s) 580, 588, 942, 1426, 2287, 3148, 3715, 4780, 6080
HindII GTYRAC 9 cut(s) 580, 588, 942, 1426, 2287, 3148, 3715, 4780, 6080
HindIII AAGCTT 7 cut(s) 784, 1802, 2663, 3524, 4091, 4355, 5126
HpaI GTTAAC 1 cut(s) 4780
Hpy99I CGWCG 6 cut(s) 1170, 1335, 2031, 2196, 2892, 3057
HpyCH4IV ACGT 4 cut(s) 770, 3583, 4734, 5889
HpyF3I CTNAG 9 cut(s) 183, 465, 594, 1211, 2072, 2933, 4353, 4916, 5204
HpySE526I ACGT 4 cut(s) 770, 3583, 4734, 5889
Hsp92I GRCGYC 3 cut(s) 812, 902, 5889
HspAI GCGC 1 cut(s) 5420
KroI GCCGGC 1 cut(s) 6120
KroNI GCCGGC 1 cut(s) 6122
Ksp22I TGATCA 5 cut(s) 1621, 2482, 3343, 3910, 5836
KspAI GTTAAC 1 cut(s) 4780
LguI GCTCTTC 6 cut(s) 659, 1638, 2499, 3360, 3927, 4280
LmnI GCTCC 8 cut(s) 1010, 1871, 2732, 4877, 5183, 5674, 5920, 6116
MaeI CTAG 6 cut(s) 71, 171, 4767, 5213, 5940, 6092
MaeII ACGT 4 cut(s) 770, 3583, 4734, 5889
MbiI CCGCTC 1 cut(s) 369
MfeI CAATTG 4 cut(s) 1340, 2201, 3062, 5774
MflI RGATCY 4 cut(s) 651, 5254, 5698, 6230
MhlI GDGCHC 3 cut(s) 4302, 4962, 5010
MlsI TGGCCA 5 cut(s) 1272, 2133, 2994, 3645, 4368
MluNI TGGCCA 5 cut(s) 1272, 2133, 2994, 3645, 4368
MlyI GAGTC 6 cut(s) 1247, 2108, 2969, 3620, 4966, 5276
Mox20I TGGCCA 5 cut(s) 1272, 2133, 2994, 3645, 4368
Mph1103I ATGCAT 6 cut(s) 1444, 2305, 3166, 3733, 4447, 5785
MroNI GCCGGC 1 cut(s) 6120
MroXI GAANNNNTTC 1 cut(s) 4289
MscI TGGCCA 5 cut(s) 1272, 2133, 2994, 3645, 4368
MslI CAYNNNNRTG 2 cut(s) 4374, 6157
Msp20I TGGCCA 5 cut(s) 1272, 2133, 2994, 3645, 4368
MspCI CTTAAG 4 cut(s) 1685, 2546, 3407, 3974
MunI CAATTG 4 cut(s) 1340, 2201, 3062, 5774
Mva1269I GAATGC 6 cut(s) 1231, 2092, 2953, 3604, 5250, 5785
NaeI GCCGGC 1 cut(s) 6122
NcoI CCATGG 2 cut(s) 4369, 4789
NgoMIV GCCGGC 1 cut(s) 6120
NheI GCTAGC 1 cut(s) 170
NlaIV GGNNCC 7 cut(s) 481, 622, 741, 4186, 5916, 6032, 6112
NmuCI GTSAC 7 cut(s) 135, 164, 196, 4538, 5073, 5559, 5738
NsiI ATGCAT 6 cut(s) 1444, 2305, 3166, 3733, 4447, 5785
NspI RCATGY 9 cut(s) 1324, 1663, 2185, 2524, 3046, 3385, 3952, 4445, 5114
NspV TTCGAA 4 cut(s) 4556, 4729, 4797, 5820
PaeI GCATGC 2 cut(s) 4445, 5114
PagI TCATGA 4 cut(s) 1842, 2703, 3564, 4131
PaqCI CACCTGC 3 cut(s) 1215, 2076, 2937
PciI ACATGT 3 cut(s) 1320, 2181, 3042
PciSI GCTCTTC 6 cut(s) 659, 1638, 2499, 3360, 3927, 4280
PctI GAATGC 6 cut(s) 1231, 2092, 2953, 3604, 5250, 5785
PdiI GCCGGC 1 cut(s) 6122
PdmI GAANNNNTTC 1 cut(s) 4289
PflFI GACNNNGTC 3 cut(s) 1279, 2140, 3001
PflMI CCANNNNNTGG 1 cut(s) 5639
PfoI TCCNGGA 1 cut(s) 4672
PleI GAGTC 6 cut(s) 1246, 2107, 2968, 3619, 4966, 5275
PpsI GAGTC 6 cut(s) 1246, 2107, 2968, 3619, 4966, 5275
PscI ACATGT 3 cut(s) 1320, 2181, 3042
PshAI GACNNNNGTC 1 cut(s) 203
PshBI ATTAAT 2 cut(s) 863, 5229
PsiI TTATAA 2 cut(s) 4503, 4952
Psp124BI GAGCTC 2 cut(s) 4302, 4962
PspEI GGTNACC 1 cut(s) 5073
PspFI CCCAGC 1 cut(s) 5080
PspN4I GGNNCC 7 cut(s) 481, 622, 741, 4186, 5916, 6032, 6112
PstI CTGCAG 3 cut(s) 880, 910, 5275
PstNI CAGNNNCTG 5 cut(s) 1279, 2140, 3001, 4668, 5279
PsuI RGATCY 4 cut(s) 651, 5254, 5698, 6230
PsyI GACNNNGTC 3 cut(s) 1279, 2140, 3001
PvuII CAGCTG 4 cut(s) 1027, 1888, 2749, 5393
RseI CAYNNNNRTG 2 cut(s) 4374, 6157
Rsr2I CGGWCCG 1 cut(s) 159
RsrII CGGWCCG 1 cut(s) 159
SacI GAGCTC 2 cut(s) 4302, 4962
SalI GTCGAC 1 cut(s) 6078
SapI GCTCTTC 6 cut(s) 659, 1638, 2499, 3360, 3927, 4280
ScaI AGTACT 1 cut(s) 4562
SchI GAGTC 6 cut(s) 1247, 2108, 2969, 3620, 4966, 5276
SduI GDGCHC 3 cut(s) 4302, 4962, 5010
SfcI CTRYAG 5 cut(s) 876, 906, 963, 5271, 5394
SfuI TTCGAA 4 cut(s) 4556, 4729, 4797, 5820
SmiMI CAYNNNNRTG 2 cut(s) 4374, 6157
SmlI CTYRAG 5 cut(s) 1685, 2546, 3407, 3974, 5564
SmoI CTYRAG 5 cut(s) 1685, 2546, 3407, 3974, 5564
SphI GCATGC 2 cut(s) 4445, 5114
SspI AATATT 1 cut(s) 4387
SspMI CTAG 6 cut(s) 71, 171, 4767, 5213, 5940, 6092
SstI GAGCTC 2 cut(s) 4302, 4962
StyI CCWWGG 8 cut(s) 1517, 2378, 3239, 3806, 4227, 4369, 4789, 4836
TaiI ACGT 4 cut(s) 773, 3586, 4737, 5892
TaqII GACCGA 3 cut(s) 1071, 1932, 2793
TatI WGTACW 3 cut(s) 441, 4560, 4815
TauI GCSGC 5 cut(s) 1693, 2554, 3415, 3982, 5915
TscAI CASTG 9 cut(s) 1284, 1608, 2145, 2469, 3006, 3330, 3897, 4572, 5416
TseFI GTSAC 7 cut(s) 135, 164, 196, 4538, 5073, 5559, 5738
Tsp45I GTSAC 7 cut(s) 135, 164, 196, 4538, 5073, 5559, 5738
TspGWI ACGGA 5 cut(s) 766, 4826, 4921, 5196, 5569
TspRI CASTG 9 cut(s) 1284, 1608, 2145, 2469, 3006, 3330, 3897, 4572, 5416
Tth111I GACNNNGTC 3 cut(s) 1279, 2140, 3001
Van91I CCANNNNNTGG 1 cut(s) 5639
Vha464I CTTAAG 4 cut(s) 1685, 2546, 3407, 3974
VspI ATTAAT 2 cut(s) 863, 5229
XbaI TCTAGA 1 cut(s) 5212
XceI RCATGY 9 cut(s) 1324, 1663, 2185, 2524, 3046, 3385, 3952, 4445, 5114
XcmI CCANNNNNNNNNTGG 2 cut(s) 3653, 5067
XmiI GTMKAC 1 cut(s) 6079
XmnI GAANNNNTTC 1 cut(s) 4289
XspI CTAG 6 cut(s) 71, 171, 4767, 5213, 5940, 6092
ZraI GACGTC 1 cut(s) 5890
ZrmI AGTACT 1 cut(s) 4562
Zsp2I ATGCAT 6 cut(s) 1444, 2305, 3166, 3733, 4447, 5785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.