MD06G1120900.v1.1

Protein BRANCHLESS TRICHOME

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009538
Physical Location & Seq
Forward (+)
26134419 .. 26135471
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1120900.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGATGAGCACCAATAGTACTCCTCCTCCTAGCCCAGGAAATACCAGAAATATTGAAATCACCACCTCCGCTTTCCCTACCTGGAAACTGTACGAAAACCCTTTTTATAATTCTCAAAAACCCCATCAAAATCCACCCCTACAACCACAAAACCAGCAGCAGCAATGCCAAAACTTTAGCAACAAGCAACCGCAACAAGAAGTTCACCATTGCCTGCACCTCCCCATCTCCGCCCGCAAGCTCGCCGCTTCCTTCTGGGATCTCACCTTCTTCAAGCCGGCAATGGAGTCCGAGATGGATTACACGCGAGCCCAGATCATCGGAATGAAAGCCGAGCTCGAGTACGAGCGGAAGGCGCGCAAGAAGTTGGAGAGTTCGAAGAAGAGGTTGGCCAACGAGTTGGGGGAGGAGAGGAGGGCAAGAGAAGCGATCGAGAGGGTTTGCGAGGAGCTTGCTAGAGAAATTTCTTTCGGGAAGTCAGAGATCACGAAGATGAGGAAAGAGATCGAGGAGGAGAGGAAAATGCTTCGAATGGCGGAGGTGCTGAGGGAGGAGAGAGTTCAGATGAAGCTCGCCGAGGCGAGACTTCTGTTCGAAGAGAAGGTGTTAGAGTTGGAGGGGTGTAAACAAATGCTGTGTGCCGCCGAGAACTCACATTTCAAGATCAAACATAAAAATGAGGTTGTTAACGTTGCTAGTTTGGATAGTTCCAGTGGAAAGTCTGGGGCTCTTTGTGGTGATCGGAACAATGATTCTGTTTCCAGTTTTTCTACTGCAACTTCAAGGGAAGTGCTTTTGGGTGAAAAAGCAGCTTTCAGTGAAAACAGCGGTGGATTTTCATCCATGGCGGTTCAGAAAAGAGCCTCACCAGAACCAGAAAATCCTCACATAAAGAGAGGGATCAAGGGGTTTGTTGAATTCCCAAGAGTTGTCAGAGCAATTGGATCAAAAAGTAGGCATTGGGGCACAAAGCTTGAGTGCCAAAAAGCTCAGCTCAGAATTCTGATGAAGCAAAAGAGCCCCATTAGATCCAATAGTTTCATTATTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0008150 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0048468 GO:0048856 GO:0048869 GO:0071840 GO:0090558 GO:0090626
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

39.93

Weight (kDa)

9.39

Isoelectric Point (pI)

57.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 108
AccBSI CCGCTC 1 cut(s) 349
AccII CGCG 2 cut(s) 307, 358
AclI AACGTT 1 cut(s) 690
AclWI GGATC 4 cut(s) 267, 908, 952, 1023
AcoI YGGCCR 1 cut(s) 390
AcsI RAATTY 3 cut(s) 462, 917, 999
AfaI GTAC 3 cut(s) 19, 92, 344
AfiI CCNNNNNNNGG 1 cut(s) 35
AgsI TTSAA 5 cut(s) 56, 274, 661, 783, 917
AjnI CCWGG 2 cut(s) 34, 80
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 8 cut(s) 241, 337, 451, 571, 812, 973, 989, 994
AluI AGCT 8 cut(s) 241, 337, 451, 571, 812, 973, 989, 994
Alw21I GWGCWC 2 cut(s) 11, 339
Alw26I GTCTC 1 cut(s) 577
AlwI GGATC 4 cut(s) 267, 908, 952, 1023
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 3 cut(s) 157, 160, 809
ApoI RAATTY 3 cut(s) 462, 917, 999
AspLEI GCGC 2 cut(s) 358, 360
AsuHPI GGTGA 6 cut(s) 52, 197, 256, 749, 812, 858
AsuII TTCGAA 3 cut(s) 377, 529, 594
AvaI CYCGRG 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 968
BalI TGGCCA 1 cut(s) 392
BanII GRGCYC 4 cut(s) 313, 339, 730, 1022
Bbv12I GWGCWC 2 cut(s) 11, 339
BbvCI CCTCAGC 1 cut(s) 545
BbvI GCAGC 3 cut(s) 169, 172, 821
BccI CCATC 3 cut(s) 132, 233, 289
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 2 cut(s) 36, 82
BcoDI GTCTC 1 cut(s) 577
BfaI CTAG 3 cut(s) 30, 456, 696
BisI GCNGC 5 cut(s) 158, 161, 246, 642, 810
BlpI GCTNAGC 1 cut(s) 990
BlsI GCNGC 5 cut(s) 159, 162, 247, 643, 811
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 2 cut(s) 36, 82
BmeT110I CYCGRG 1 cut(s) 338
BmrFI CCNGG 2 cut(s) 36, 82
Bpu10I CCTNAGC 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 990
Bpu14I TTCGAA 3 cut(s) 377, 529, 594
BpuEI CTTGAG 1 cut(s) 995
BsaBI GATNNNNATC 1 cut(s) 838
BsaJI CCNNGG 3 cut(s) 34, 576, 843
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 711, 762
Bse3DI GCAATG 3 cut(s) 170, 208, 288
Bse8I GATNNNNATC 1 cut(s) 838
BseBI CCWGG 2 cut(s) 36, 82
BseDI CCNNGG 3 cut(s) 34, 576, 843
BseGI GGATG 1 cut(s) 839
BseJI GATNNNNATC 1 cut(s) 838
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMI GCAATG 3 cut(s) 170, 208, 288
BseMII CTCAG 3 cut(s) 536, 1004, 1009
BseNI ACTGG 2 cut(s) 711, 762
BsePI GCGCGC 1 cut(s) 356
BseRI GAGGAG 8 cut(s) 12, 15, 422, 427, 461, 524, 527, 566
BseSI GKGCMC 1 cut(s) 968
BseXI GCAGC 3 cut(s) 169, 172, 821
BsgI GTGCAG 1 cut(s) 200
Bsh1236I CGCG 2 cut(s) 307, 358
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 1 cut(s) 392
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 2 cut(s) 11, 339
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 577
BsnI GGCC 1 cut(s) 392
BsoBI CYCGRG 1 cut(s) 338
Bsp119I TTCGAA 3 cut(s) 377, 529, 594
Bsp1286I GDGCHC 6 cut(s) 11, 313, 339, 730, 968, 1022
Bsp1720I GCTNAGC 1 cut(s) 990
Bsp19I CCATGG 1 cut(s) 843
BspANI GGCC 1 cut(s) 392
BspCNI CTCAG 3 cut(s) 537, 1003, 1008
BspFNI CGCG 2 cut(s) 307, 358
BspPI GGATC 4 cut(s) 267, 908, 952, 1023
BspT104I TTCGAA 3 cut(s) 377, 529, 594
BsrBI CCGCTC 1 cut(s) 349
BsrDI GCAATG 3 cut(s) 170, 208, 288
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 2 cut(s) 711, 762
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 3 cut(s) 34, 576, 843
BssHII GCGCGC 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 843
Bst2UI CCWGG 2 cut(s) 36, 82
Bst4CI ACNGT 1 cut(s) 90
Bst6I CTCTTC 2 cut(s) 377, 591
BstBI TTCGAA 3 cut(s) 377, 529, 594
BstC8I GCNNGC 9 cut(s) 215, 235, 239, 243, 279, 309, 358, 453, 573
BstDEI CTNAG 3 cut(s) 545, 990, 995
BstDSI CCRYGG 1 cut(s) 843
BstENI CCTNNNNNAGG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 839
BstFNI CGCG 2 cut(s) 307, 358
BstHHI GCGC 2 cut(s) 358, 360
BstMAI GTCTC 1 cut(s) 577
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 2 cut(s) 355, 425
BstNI CCWGG 2 cut(s) 36, 82
BstSCI CCNGG 2 cut(s) 34, 80
BstSLI GKGCMC 1 cut(s) 968
BstUI CGCG 2 cut(s) 307, 358
BstV1I GCAGC 3 cut(s) 169, 172, 821
BstX2I RGATCY 2 cut(s) 259, 1028
BstXI CCANNNNNNTGG 1 cut(s) 400
BstYI RGATCY 2 cut(s) 259, 1028
BsuRI GGCC 1 cut(s) 392
BtgI CCRYGG 1 cut(s) 843
BtsCI GGATG 1 cut(s) 839
BtsIMutI CAGTG 2 cut(s) 718, 823
Cac8I GCNNGC 9 cut(s) 215, 235, 239, 243, 279, 309, 358, 453, 573
CfoI GCGC 2 cut(s) 358, 360
Cfr10I RCCGGY 1 cut(s) 277
Csp6I GTAC 3 cut(s) 18, 91, 343
CviAII CATG 1 cut(s) 844
CviQI GTAC 3 cut(s) 18, 91, 343
DdeI CTNAG 3 cut(s) 545, 990, 995
EaeI YGGCCR 1 cut(s) 390
Eam1104I CTCTTC 2 cut(s) 377, 591
EarI CTCTTC 2 cut(s) 377, 591
EciI GGCGGA 2 cut(s) 220, 551
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 843
Eco24I GRGCYC 4 cut(s) 313, 339, 730, 1022
Eco53kI GAGCTC 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 33
EcoRI GAATTC 2 cut(s) 917, 999
EcoRII CCWGG 2 cut(s) 34, 80
EcoT14I CCWWGG 1 cut(s) 843
EcoT38I GRGCYC 4 cut(s) 313, 339, 730, 1022
ErhI CCWWGG 1 cut(s) 843
FaeI CATG 1 cut(s) 847
FaiI YATR 4 cut(s) 108, 672, 845, 890
FatI CATG 1 cut(s) 843
FauI CCCGC 1 cut(s) 242
Fnu4HI GCNGC 5 cut(s) 158, 161, 246, 642, 810
FokI GGATG 1 cut(s) 826
FriOI GRGCYC 4 cut(s) 313, 339, 730, 1022
Fsp4HI GCNGC 5 cut(s) 158, 161, 246, 642, 810
FspBI CTAG 3 cut(s) 30, 456, 696
GlaI GCGC 2 cut(s) 357, 359
GluI GCNGC 5 cut(s) 158, 161, 246, 642, 810
HaeIII GGCC 1 cut(s) 392
HapII CCGG 1 cut(s) 278
HhaI GCGC 2 cut(s) 358, 360
Hin1II CATG 1 cut(s) 847
Hin6I GCGC 2 cut(s) 356, 358
HinP1I GCGC 2 cut(s) 356, 358
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 1 cut(s) 971
HinfI GANTC 2 cut(s) 287, 752
HpaI GTTAAC 1 cut(s) 688
HpaII CCGG 1 cut(s) 278
HphI GGTGA 6 cut(s) 52, 197, 256, 749, 812, 858
Hpy166II GTNNAC 3 cut(s) 205, 626, 688
Hpy188I TCNGA 9 cut(s) 292, 323, 481, 564, 744, 855, 935, 998, 1005
Hpy188III TCNNGA 4 cut(s) 433, 472, 487, 661
Hpy8I GTNNAC 3 cut(s) 205, 626, 688
HpyAV CCTTC 4 cut(s) 262, 277, 346, 595
HpyCH4III ACNGT 1 cut(s) 90
HpyCH4IV ACGT 1 cut(s) 690
HpyCH4V TGCA 2 cut(s) 217, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 355, 425
HpyF3I CTNAG 3 cut(s) 545, 990, 995
HpySE526I ACGT 1 cut(s) 690
Hsp92II CATG 1 cut(s) 847
HspAI GCGC 2 cut(s) 356, 358
KroI GCCGGC 1 cut(s) 277
KroNI GCCGGC 1 cut(s) 279
KspAI GTTAAC 1 cut(s) 688
LmnI GCTCC 1 cut(s) 448
Lsp1109I GCAGC 3 cut(s) 169, 172, 821
MaeI CTAG 3 cut(s) 30, 456, 696
MaeII ACGT 1 cut(s) 690
MbiI CCGCTC 1 cut(s) 349
MboII GAAGA 5 cut(s) 262, 391, 394, 502, 608
MfeI CAATTG 1 cut(s) 939
MflI RGATCY 2 cut(s) 259, 1028
MhlI GDGCHC 6 cut(s) 11, 313, 339, 730, 968, 1022
MlsI TGGCCA 1 cut(s) 392
MluCI AATT 5 cut(s) 109, 462, 917, 939, 999
MluNI TGGCCA 1 cut(s) 392
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 2 cut(s) 348, 594
Mox20I TGGCCA 1 cut(s) 392
MroNI GCCGGC 1 cut(s) 277
MscI TGGCCA 1 cut(s) 392
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 3 cut(s) 323, 491, 675
Msp20I TGGCCA 1 cut(s) 392
MspA1I CMGCKG 1 cut(s) 828
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 2 cut(s) 36, 82
MunI CAATTG 1 cut(s) 939
MvaI CCWGG 2 cut(s) 36, 82
MvnI CGCG 2 cut(s) 307, 358
MwoI GCNNNNNNNGC 2 cut(s) 355, 425
NaeI GCCGGC 1 cut(s) 279
NcoI CCATGG 1 cut(s) 843
NgoMIV GCCGGC 1 cut(s) 277
NlaIII CATG 1 cut(s) 847
NmeAIII GCCGAG 3 cut(s) 358, 601, 670
NspV TTCGAA 3 cut(s) 377, 529, 594
PaeR7I CTCGAG 1 cut(s) 338
PauI GCGCGC 1 cut(s) 356
PdiI GCCGGC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 752
PkrI GCNGC 5 cut(s) 159, 162, 247, 643, 811
Ple19I CGATCG 1 cut(s) 432
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
PsiI TTATAA 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 2 cut(s) 34, 80
PspGI CCWGG 2 cut(s) 34, 80
PspXI VCTCGAGB 1 cut(s) 338
PsuI RGATCY 2 cut(s) 259, 1028
PteI GCGCGC 1 cut(s) 356
PvuI CGATCG 1 cut(s) 432
RsaI GTAC 3 cut(s) 19, 92, 344
RsaNI GTAC 3 cut(s) 18, 91, 343
RseI CAYNNNNRTG 3 cut(s) 323, 491, 675
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 687
SatI GCNGC 5 cut(s) 158, 161, 246, 642, 810
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 36, 82
SduI GDGCHC 6 cut(s) 11, 313, 339, 730, 968, 1022
Sfr274I CTCGAG 1 cut(s) 338
SfuI TTCGAA 3 cut(s) 377, 529, 594
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 3 cut(s) 323, 491, 675
SmlI CTYRAG 2 cut(s) 338, 974
SmoI CTYRAG 2 cut(s) 338, 974
Sse9I AATT 5 cut(s) 109, 462, 917, 939, 999
SspI AATATT 1 cut(s) 52
SspMI CTAG 3 cut(s) 30, 456, 696
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 2 cut(s) 34, 80
StyI CCWWGG 1 cut(s) 843
TaaI ACNGT 1 cut(s) 90
TaiI ACGT 1 cut(s) 693
TaqI TCGA 6 cut(s) 339, 377, 432, 507, 529, 594
TasI AATT 5 cut(s) 109, 462, 917, 939, 999
TatI WGTACW 1 cut(s) 17
TauI GCSGC 2 cut(s) 248, 644
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 2 cut(s) 718, 823
TseI GCWGC 3 cut(s) 157, 160, 809
TspDTI ATGAA 5 cut(s) 341, 581, 828, 1022, 1030
TspRI CASTG 2 cut(s) 718, 823
XagI CCTNNNNNAGG 1 cut(s) 33
XapI RAATTY 3 cut(s) 462, 917, 999
XhoI CTCGAG 1 cut(s) 338
XspI CTAG 3 cut(s) 30, 456, 696
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.