Rorug06G0481100

Protein BRANCHLESS TRICHOME

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009538
Physical Location & Seq
Forward (+)
60263385 .. 60263883
499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0481100.1

Sequence Viewer

Length: 417 bp
ATGTTCCGGAACGTGCTACGTGTCATTATCCCAGTGACTCCGAATCTCCGTCACATCAGCCCCCAAACTGGGACCAAACACTATGTTGGGCCGCTCGAGTCTCTTAGTAAGATCCAAGCCCATGATCCGCCTTTCACGGAGGATTTGCCTAGACTTGATGCGCCCAATTTCTACCACACTCTGGAGGACATACTTTTCGAGGACGTTAAGAAGAAAGAGGGCCTGACTTGGTCGGTGAACCGGCCCAATAACATTTCAGGCAGCAAAGAAGTTTGGGAATCTTACGCGGTGGCGGCTGATGCAGATCTGATTGCGGAGCAGCATATATGGACCGCGGTGGATCCAAATGCCAAAAACGAAGCGTTTAACATCAATAATGGGGATCACGTGGAAGAAACTTTGGAAGGTGTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0008150 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0048468 GO:0048856 GO:0048869 GO:0071840 GO:0090558 GO:0090626
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.51

Weight (kDa)

5.0

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 3 - 87 2.2e-06 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 181
AccBSI CCGCTC 1 cut(s) 94
AccII CGCG 2 cut(s) 287, 335
AccIII TCCGGA 1 cut(s) 6
AciI CCGC 7 cut(s) 92, 128, 287, 293, 314, 333, 335
AclWI GGATC 5 cut(s) 106, 119, 335, 348, 390
AcvI CACGTG 1 cut(s) 388
AfiI CCNNNNNNNGG 3 cut(s) 68, 69, 181
AflIII ACRYGT 1 cut(s) 19
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 5 cut(s) 106, 119, 335, 348, 390
Ama87I CYCGRG 1 cut(s) 95
Aor13HI TCCGGA 1 cut(s) 6
AoxI GGCC 3 cut(s) 89, 220, 242
ApeKI GCWGC 2 cut(s) 261, 319
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 5 cut(s) 72, 89, 220, 243, 330
AsuHPI GGTGA 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 2 cut(s) 72, 330
BamHI GGATCC 1 cut(s) 340
BbrPI CACGTG 1 cut(s) 388
BbvI GCAGC 2 cut(s) 273, 331
BcoDI GTCTC 1 cut(s) 105
BfaI CTAG 1 cut(s) 150
BglII AGATCT 1 cut(s) 304
BisI GCNGC 4 cut(s) 92, 262, 294, 320
BlsI GCNGC 4 cut(s) 93, 263, 295, 321
Bme18I GGWCC 2 cut(s) 72, 330
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 5 cut(s) 72, 89, 220, 243, 330
BmiI GGNNCC 2 cut(s) 73, 342
BmrI ACTGGG 2 cut(s) 26, 78
BmsI GCATC 2 cut(s) 148, 289
BmuI ACTGGG 2 cut(s) 26, 78
BpmI CTGGAG 1 cut(s) 203
BsaAI YACGTR 2 cut(s) 20, 388
BsaBI GATNNNNATC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 6
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 69, 181
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 2 cut(s) 32, 73
Bse8I GATNNNNATC 1 cut(s) 303
BseAI TCCGGA 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 333
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 3 cut(s) 68, 69, 181
BseNI ACTGG 2 cut(s) 32, 73
BseXI GCAGC 2 cut(s) 273, 331
Bsh1236I CGCG 2 cut(s) 287, 335
BshFI GGCC 3 cut(s) 91, 222, 244
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 7, 241
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 3 cut(s) 68, 69, 181
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 3 cut(s) 91, 222, 244
BsoBI CYCGRG 1 cut(s) 95
Bsp13I TCCGGA 1 cut(s) 6
Bsp143I GATC 5 cut(s) 111, 124, 304, 340, 382
BspACI CCGC 7 cut(s) 92, 128, 287, 293, 314, 333, 335
BspANI GGCC 3 cut(s) 91, 222, 244
BspEI TCCGGA 1 cut(s) 6
BspFNI CGCG 2 cut(s) 287, 335
BspLI GGNNCC 2 cut(s) 73, 342
BspPI GGATC 5 cut(s) 106, 119, 335, 348, 390
BsrBI CCGCTC 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 2 cut(s) 32, 73
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 5 cut(s) 111, 124, 304, 340, 382
BstBAI YACGTR 2 cut(s) 20, 388
BstDEI CTNAG 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 333
BstFNI CGCG 2 cut(s) 287, 335
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 5 cut(s) 114, 127, 307, 343, 385
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 5 cut(s) 111, 124, 304, 340, 382
BstMWI GCNNNNNNNGC 2 cut(s) 293, 299
BstUI CGCG 2 cut(s) 287, 335
BstV1I GCAGC 2 cut(s) 273, 331
BstX2I RGATCY 3 cut(s) 111, 304, 340
BstYI RGATCY 3 cut(s) 111, 304, 340
BsuRI GGCC 3 cut(s) 91, 222, 244
BtgI CCRYGG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 39
CfoI GCGC 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 5 cut(s) 72, 89, 220, 243, 330
Cfr42I CCGCGG 1 cut(s) 336
CviAII CATG 1 cut(s) 122
CviJI RGCY 7 cut(s) 60, 91, 119, 222, 244, 296, 414
CviKI_1 RGCY 7 cut(s) 60, 91, 119, 222, 244, 296, 414
DdeI CTNAG 1 cut(s) 104
DpnI GATC 5 cut(s) 113, 126, 306, 342, 384
DpnII GATC 5 cut(s) 111, 124, 304, 340, 382
EciI GGCGGA 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 72, 330
Eco72I CACGTG 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 220
FaeI CATG 1 cut(s) 125
FaiI YATR 6 cut(s) 84, 123, 191, 324, 326, 328
FaqI GGGAC 1 cut(s) 85
FatI CATG 1 cut(s) 121
Fnu4HI GCNGC 4 cut(s) 92, 262, 294, 320
Fsp4HI GCNGC 4 cut(s) 92, 262, 294, 320
FspBI CTAG 1 cut(s) 150
GlaI GCGC 1 cut(s) 162
GluI GCNGC 4 cut(s) 92, 262, 294, 320
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 3 cut(s) 91, 222, 244
HapII CCGG 2 cut(s) 7, 241
HhaI GCGC 1 cut(s) 163
Hin1II CATG 1 cut(s) 125
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 4 cut(s) 37, 43, 98, 278
HpaII CCGG 2 cut(s) 7, 241
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 2 cut(s) 42, 309
Hpy188III TCNNGA 2 cut(s) 7, 182
Hpy8I GTNNAC 1 cut(s) 238
HpyAV CCTTC 1 cut(s) 398
HpyCH4IV ACGT 4 cut(s) 12, 19, 204, 387
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 299
HpyF3I CTNAG 1 cut(s) 104
HpySE526I ACGT 4 cut(s) 12, 19, 204, 387
Hsp92II CATG 1 cut(s) 125
HspAI GCGC 1 cut(s) 161
Kpn2I TCCGGA 1 cut(s) 6
KspI CCGCGG 1 cut(s) 336
Kzo9I GATC 5 cut(s) 111, 124, 304, 340, 382
LmnI GCTCC 1 cut(s) 316
LpnPI CCDG 7 cut(s) 20, 45, 54, 167, 236, 243, 254
Lsp1109I GCAGC 2 cut(s) 273, 331
LweI GCATC 2 cut(s) 148, 289
MaeI CTAG 1 cut(s) 150
MaeII ACGT 4 cut(s) 12, 19, 204, 387
MaeIII GTNAC 2 cut(s) 34, 50
MalI GATC 5 cut(s) 113, 126, 306, 342, 384
MbiI CCGCTC 1 cut(s) 94
MboI GATC 5 cut(s) 111, 124, 304, 340, 382
MboII GAAGA 2 cut(s) 223, 404
MflI RGATCY 3 cut(s) 111, 304, 340
MluCI AATT 1 cut(s) 166
MlyI GAGTC 2 cut(s) 31, 107
MnlI CCTC 4 cut(s) 133, 178, 193, 211
MroI TCCGGA 1 cut(s) 6
MseI TTAA 2 cut(s) 207, 366
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 2 cut(s) 7, 241
MvnI CGCG 2 cut(s) 287, 335
MwoI GCNNNNNNNGC 2 cut(s) 293, 299
NdeII GATC 5 cut(s) 111, 124, 304, 340, 382
NlaIII CATG 1 cut(s) 125
NlaIV GGNNCC 2 cut(s) 73, 342
NmuCI GTSAC 2 cut(s) 34, 50
PaeR7I CTCGAG 1 cut(s) 95
PfeI GAWTC 2 cut(s) 43, 278
PflFI GACNNNGTC 1 cut(s) 229
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 4 cut(s) 93, 263, 295, 321
PleI GAGTC 2 cut(s) 31, 106
PmaCI CACGTG 1 cut(s) 388
PmlI CACGTG 1 cut(s) 388
PpsI GAGTC 2 cut(s) 31, 106
Ppu21I YACGTR 2 cut(s) 20, 388
PspCI CACGTG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 73, 342
PspPI GGNCC 5 cut(s) 72, 89, 220, 243, 330
PspXI VCTCGAGB 1 cut(s) 95
PsuI RGATCY 3 cut(s) 111, 304, 340
PsyI GACNNNGTC 1 cut(s) 229
SacII CCGCGG 1 cut(s) 336
SaqAI TTAA 2 cut(s) 207, 366
SatI GCNGC 4 cut(s) 92, 262, 294, 320
Sau3AI GATC 5 cut(s) 111, 124, 304, 340, 382
Sau96I GGNCC 5 cut(s) 72, 89, 220, 243, 330
SchI GAGTC 2 cut(s) 31, 107
SetI ASST 5 cut(s) 15, 22, 207, 390, 409
SfaNI GCATC 2 cut(s) 148, 289
Sfr274I CTCGAG 1 cut(s) 95
Sfr303I CCGCGG 1 cut(s) 336
SgrBI CCGCGG 1 cut(s) 336
SinI GGWCC 2 cut(s) 72, 330
SlaI CTCGAG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 1 cut(s) 166
SsiI CCGC 7 cut(s) 92, 128, 287, 293, 314, 333, 335
SspMI CTAG 1 cut(s) 150
TaiI ACGT 4 cut(s) 15, 22, 207, 390
TaqI TCGA 2 cut(s) 96, 198
TasI AATT 1 cut(s) 166
TauI GCSGC 2 cut(s) 94, 296
TfiI GAWTC 2 cut(s) 43, 278
Tru1I TTAA 2 cut(s) 207, 366
Tru9I TTAA 2 cut(s) 207, 366
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 2 cut(s) 34, 50
TseI GCWGC 2 cut(s) 261, 319
Tsp45I GTSAC 2 cut(s) 34, 50
TspGWI ACGGA 2 cut(s) 38, 152
TspRI CASTG 1 cut(s) 39
Tth111I GACNNNGTC 1 cut(s) 229
Van91I CCANNNNNTGG 1 cut(s) 181
VpaK11BI GGWCC 2 cut(s) 72, 330
XhoI CTCGAG 1 cut(s) 95
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.