Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010875

Physical Location & Seq

Genomic Coordinates Forward (+)

31263658 .. 31268996

Gene Structure 5339 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1172500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3423 bp

ATGATTAATGGAGAGAAGATTGCCGAGGCTGCCTCTCCCGATCCGTCCATCCCGTTCGATCCCAAATCGGACGGCGTAGGTGACGGTGGTGATTGCGATGGCGATGTTTCGCCCCGATCGCCTCCGTCGCGCTACTCCTCGTTCGGCGAGTCCGAGTACGAGCGCTATTGCAGCGCCAACTCGATCATGGGCACGCCGAGCATGTGTAGCACAATCACGGTTTTCAATGATTTCCCGGAGCCCGAATTTGGGTCGTTGAAAAGTTTGGGACTTGGGGAGGGGAGTGGTGGTTTGGATAATTTTAGCTTGGGAGGGAGGATTGAGAGAAATCGAGAGGATCGGAGATGTTTGAGTAGTGGTAGGATTGAGTTTGGGAAGGAGGATGGTAATATTGGAGGACGGAGGAGGGCGAGTTACGGGTCTAGTGGGTTGGAATTGTATGGCAATGAGGATGATGGTGGAGCTGATGATGTAAATGAATTGATGTCTTGGAAGCTTGAGAGTGGATCGTCGGGTTTAAGGGGGGTTTCGGATGTGAAGTATGGTTCGGATAATAGCGACGAGGACAGTGAAAAGGGTATGGAAGTTTGGAGGGGAGTTGTGGGAAATGATAGTATTGGAGTTGAAGGTGTGGCAGCCCAAGAGACTAATGATTCGAATGGGGAAGGAATTCGGAACCAGTTTGTACCTGAAGTTGAAGAATTTGATGGAAGAGAGATGGATAGGGAAGAAGGGACATCATCTAGAAATGAGTATTCAGAGGATGAGGGGTCTATGTACAACTATGGTTCGGATGATGAGCGCAAGAGCGGCTTTAGTCAGCAGAGGAATGTCCACTATTATCAGCAAGAGAAACCCCAGAATGAAAATCCGTTTCTTATTAACTCATCCGTAGCCTTTGGTTCCGATGATTGGGATGATTTCATGCAAGAAAGTGGCGGGAGTAATTTAGATTCTTTCACTAGGAACGTATTTGAGGACAGGAGAGGACGGAATGTTGAAACCAAAAGGAAAATTTCGAATTCCACTTCCATCACTTCAGTTGAGGATCAGAATGCATGTCAAACCGAGCAAGGAAATGATGTGAATGTTGTGCAGCCTGGATGCAAACAAGTTCAAGCTGATGATAAATTGGTAGAAAATGTTAACAGTTCCATGAAGCTAGCTAGTTCTCCAAGTTTTCTTGAAACCGATAGAGTAGTAGATGTGAAGGACATTCCTGTGGCCAGTTATCAAGTTCAAGCTATTGATGATTCGGTTGAGTTCACCAAAAGTTCTTTTACGACCCCTTTTCAAAATGTTCAAGAACCAGAGGTAAAAGATTCAAGAGATATGCTTTTCACCAAGAATCAAGCTCCAGGTCCTGACGAATCAGCAAAACATAATAAGGCTTCCTTGGTTGGCAATGTCTTCAATATTCAGCCGGATCCACAAGCAAAAGAAAGTCCTGACAAAAAGGGTTTGAGCATTTTGGATGATGGTGTTTCTGATGTGCATAAGTATATGAATACCGATGAAGTCATTGATATTGATCACGGTCAAGATTTAGAAAAGAAAAAGTTGGGGACTTTAAAAGTTAAACTGGACCCCCTTTCTGATCAATCAACTAATCAAATTAGCATCCATTCAACCAGAACTTCGGGAAATATGGAGACAGACTTCCTTGAGGACCATAAACCAAGTACATCGCCATCAATTTTTGGAAATAATACCACAAAAAGTTCTGTTTCAGAAGATATCCTTGAAGAATATCCTATGCCAGTTAAGACGGATAATTCTGAGCATAATGAATTCTATGATGAGGTTGTTAATGAAATGGAGGAAATTCTGCTCGACTCTGCTGAGTCCCCAGGAGCAAGGTTCACTCATGGCAACAGTTACTTACAATCACAGCAACCTCTACCAGTAAGAGATGGTGGGTCGACTGCTTCTACTTCTGGTACAGATGATGCTCATTTGTTCAATCAGCATTCGTTAAGAATTGATGGGGTCGAAGTGGTGGGTGCAAGGCAGAAGAAGGGAGATGTCTCATTTAGTGAAAGACTGGTGGGAGTGAAAGAATACACTGTGTATCAAATAAGAGTGTTGAGTGGCGAGGATCATTGGGAGGTTGAACGTCGTTATCGTGATTTCTTTACACTCTATCGTCGATTGAAAACAGTGTTTTCTGACCACGGCTGGGATCTACCTTCTCCCTGGTCTGCTGTAGAAAAGGAATCCAGAAAGATTTTTGGAAATGCGTCTCCTGATGTTATTGCAGAGAGAAGTGTTCTCATCCAAGAGTGTCTACAGTCCGTTCTCCATTTCAGGTTCTTTTCCAGTCCGCCGAGTGCACTCGTATGGTTTCTGTCTGCTCAAGATTCAGTTCCTAGCTCCCTGGAATCATCATACACACCTGAGTCTCTTACTAGAAGAGCTGACACACAAAATATTTCCACTTGGGGAAAGACCATATCTCTTATTGTTGAAATTCGACAATCCAAATCTTTGAAGCAGATGCTAGAAGCACAATATTATACTTGTGCTGGGTGCCACAAGCATTTTGATGATGGCAAGACTTTGATACGGGATTTTGCACAGACACTTGGATGGGGCAAGCCTCGACTTTGTGAATATACTGGTCAATTATTTTGCTCTTCATGCCATACAAATGAAATTGCAATCATACCGGCAAGAGTGTTGCATAACTGGGACTTTACTAAATATCCAGTTTCGCAATATGCTAAATCTTATCTGGATTCTATACATGACCAGCCCATGCTTTGCGTCAGTGCAGTTAATCCGTTCCTTTTCTCGAAAGTCCCAGCCTTGCTTCATGTTATGGGTGTGAGGAAGAAAATAGGCAACATTCTTCCTTATGTTCGTTGTCCATTCCGTAGGTCAATCAACAAAGGACTTGGGTCTCGAAGATATCTTCTAGAAGGCAATGATTTTTTTGCACTGAGAGACCTCATTGATCTTTCTAAAGGAGCCTTTGCTGTGCTGCCCGTGATTGTAGAAACTGTCTCAAGGAAAATTCTGGGCCATATAACGGAGCAGTGTCTCATATGCTGCGATGTGGGAGTCCCCTGTGGGGCACGGCAAGCATGCAATGACCCTTCATCTCTCATTTTCCCTTTTCAGGAGGATGAGATCGAAAGGTGTCCATCTTGCGCATCAGTATTCCACAAGCCTTGCTTCAGAAAGATCATGGAATGCACTTGTGGCGCACACCTCAGGGAAGACGAGCCAGCGCAGCTCATTAGAAGGGCAACCTCTGGGGTCGGGCCTGAGATCAGTGGTTTTCTGGACTTGTTTGGGGGAGGTTCGGGTTCCGGATTACTCTCTGGATTGTTTTCAAAGGCGAAGCCGGAGAAGCCAAGGGAACATAAAGACGGCGACAACGTCATTTTAATGGGTTCGTTGCCAAGCACTTCTTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0009987 GO:0012505 GO:0030154 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045445 GO:0048856 GO:0048869 GO:0061061

KEGG Pathways

Metabolic & Signaling

ko04140 map04140

Pfam Domains

Protein Families

PX zf-RING_9

Protein Analysis

1141

Amino Acids

126.18

Weight (kDa)

4.89

Isoelectric Point (pI)

51.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PX	PF00787	697 - 776	6.5e-06	PX domain
RH_dom	PF13901	868 - 1070	3.4e-54	Rubicon Homology Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010875)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48195
fragaria_vesca	FvH4_5g05470 FvH4_5g05470 FvH4_5g05470
malus_domestica	MD06G1172500.v1.1 MD14G1178400.v1.1
prunus_persica	Prupe.5G171800_v2.0.a1 Prupe.5G171800_v2.0.a1
pyrus_communis	pycom06g15360 pycom14g14870
rosa_chinensis	RchiOBHm_Chr7g0193371
rosa_laevigata	RLG00000004275
rosa_multiflora	Rmu_co8475531.1_g000001
rosa_roxburghii	Rroxscaffold_3G00262170
rosa_rugosa	Rorug07G0009200
rosa_samantha	Rh7AG135500 Rh7BG135800 Rh7CG139200 Rh7DG137200
rosa_wichuraiana	Rw7G011550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3421
Acc16I	TGCGCA	1 cut(s)	3154
AccB1I	GGYRCC	1 cut(s)	2529
AccBSI	CCGCTC	1 cut(s)	810
AccI	GTMKAC	2 cut(s)	1922, 2287
AccII	CGCG	1 cut(s)	130
AccIII	TCCGGA	1 cut(s)	3314
AciI	CCGC	3 cut(s)	810, 939, 2324
AclWI	GGATC	9 cut(s)	35, 53, 345, 514, 1056, 1421, 1434, 2106, 2190
AcoI	YGGCCR	1 cut(s)	1224
AcsI	RAATTY	9 cut(s)	245, 669, 701, 1014, 1021, 1790, 1824, 2469, 3015
AcuI	CTGAAG	3 cut(s)	711, 1023, 3163
AfaI	GTAC	5 cut(s)	158, 687, 779, 1684, 1942
AfeI	AGCGCT	1 cut(s)	164
AfiI	CCNNNNNNNGG	7 cut(s)	248, 249, 912, 2179, 3031, 3073, 3121
AjnI	CCWGG	5 cut(s)	1099, 1357, 1849, 2195, 2376
Alw21I	GWGCWC	1 cut(s)	2335
Alw26I	GTCTC	9 cut(s)	638, 1646, 2032, 2247, 2406, 2907, 2940, 3010, 3047
Alw44I	GTGCAC	1 cut(s)	2331
AlwI	GGATC	9 cut(s)	35, 53, 345, 514, 1056, 1421, 1434, 2106, 2190
AlwNI	CAGNNNCTG	1 cut(s)	1364
Aor13HI	TCCGGA	1 cut(s)	3314
Aor51HI	AGCGCT	1 cut(s)	164
AoxI	GGCC	3 cut(s)	1224, 3022, 3266
ApaLI	GTGCAC	1 cut(s)	2331
ApeKI	GCWGC	7 cut(s)	29, 171, 635, 1096, 2983, 3051, 3235
ApoI	RAATTY	9 cut(s)	245, 669, 701, 1014, 1021, 1790, 1824, 2469, 3015
ArsI	GACNNNNNNTTYG	2 cut(s)	637, 669
AseI	ATTAAT	1 cut(s)	6
Asp700I	GAANNNNTTC	1 cut(s)	669
AspLEI	GCGC	7 cut(s)	132, 165, 176, 804, 3155, 3209, 3235
AspS9I	GGNCC	5 cut(s)	1361, 1585, 1669, 3022, 3266
AsuC2I	CCSGG	1 cut(s)	236
AsuHPI	GGTGA	4 cut(s)	92, 101, 1258, 1333
AsuII	TTCGAA	2 cut(s)	656, 1019
AsuNHI	GCTAGC	1 cut(s)	1162
AvaII	GGWCC	3 cut(s)	1361, 1585, 1669
AxyI	CCTNAGG	1 cut(s)	3215
BaeGI	GKGCMC	3 cut(s)	194, 2335, 3079
BalI	TGGCCA	1 cut(s)	1226
BamHI	GGATCC	1 cut(s)	1426
BanI	GGYRCC	1 cut(s)	2529
BanII	GRGCYC	1 cut(s)	243
BbsI	GAAGAC	2 cut(s)	1402, 3228
Bbv12I	GWGCWC	1 cut(s)	2335
BbvI	GCAGC	7 cut(s)	16, 183, 647, 1108, 2970, 3038, 3247
BceAI	ACGGC	4 cut(s)	88, 2191, 3095, 3391
BciT130I	CCWGG	5 cut(s)	1101, 1359, 1851, 2197, 2378
BclI	TGATCA	2 cut(s)	1531, 1597
BcnI	CCSGG	1 cut(s)	236
BcoDI	GTCTC	9 cut(s)	638, 1646, 2032, 2247, 2406, 2907, 2940, 3010, 3047
BfaI	CTAG	9 cut(s)	423, 744, 963, 1163, 1167, 2370, 2409, 2501, 2918
BfmI	CTRYAG	2 cut(s)	2205, 2288
BfoI	RGCGCY	2 cut(s)	166, 177
BisI	GCNGC	8 cut(s)	30, 172, 636, 811, 1097, 2984, 3052, 3236
BlsI	GCNGC	8 cut(s)	31, 173, 637, 812, 1098, 2985, 3053, 3237
Bme1390I	CCNGG	6 cut(s)	236, 1101, 1359, 1851, 2197, 2378
Bme18I	GGWCC	3 cut(s)	1361, 1585, 1669
BmgT120I	GGNCC	5 cut(s)	1361, 1585, 1669, 3022, 3266
BmiI	GGNNCC	8 cut(s)	240, 677, 904, 1428, 1587, 2531, 2971, 3313
BmrFI	CCNGG	6 cut(s)	236, 1101, 1359, 1851, 2197, 2378
BmrI	ACTGGG	1 cut(s)	2698
BmsI	GCATC	5 cut(s)	1094, 1629, 1939, 2487, 3164
BmtI	GCTAGC	1 cut(s)	1166
BmuI	ACTGGG	1 cut(s)	2698
BoxI	GACNNNNGTC	1 cut(s)	2899
BpiI	GAAGAC	2 cut(s)	1402, 3228
BplI	GAGNNNNNCTC	2 cut(s)	17, 49
BpmI	CTGGAG	1 cut(s)	1341
Bpu14I	TTCGAA	2 cut(s)	656, 1019
BpuEI	CTTGAG	4 cut(s)	518, 1685, 2340, 2992
BpuMI	CCSGG	1 cut(s)	236
BsaBI	GATNNNNATC	1 cut(s)	1530
BsaI	GGTCTC	2 cut(s)	2907, 2940
BsaJI	CCNNGG	7 cut(s)	24, 1395, 1849, 2173, 2195, 2376, 3359
BsaWI	WCCGGW	1 cut(s)	3314
BsaXI	ACNNNNNCTCC	4 cut(s)	274, 304, 397, 427
Bsc4I	CCNNNNNNNGG	7 cut(s)	248, 249, 912, 2179, 3031, 3073, 3121
Bse118I	RCCGGY	1 cut(s)	2668
Bse21I	CCTNAGG	1 cut(s)	3215
Bse3DI	GCAATG	4 cut(s)	451, 1411, 2932, 3097
Bse8I	GATNNNNATC	1 cut(s)	1530
BseAI	TCCGGA	1 cut(s)	3314
BseBI	CCWGG	5 cut(s)	1101, 1359, 1851, 2197, 2378
BseDI	CCNNGG	7 cut(s)	24, 1395, 1849, 2173, 2195, 2376, 3359
BseJI	GATNNNNATC	1 cut(s)	1530
BseLI	CCNNNNNNNGG	7 cut(s)	248, 249, 912, 2179, 3031, 3073, 3121
BseMI	GCAATG	4 cut(s)	451, 1411, 2932, 3097
BseMII	CTCAG	6 cut(s)	1770, 1833, 2388, 2933, 3229, 3261
BseRI	GAGGAG	2 cut(s)	127, 418
BseSI	GKGCMC	3 cut(s)	194, 2335, 3079
BseXI	GCAGC	7 cut(s)	16, 183, 647, 1108, 2970, 3038, 3247
BseYI	CCCAGC	3 cut(s)	2178, 2525, 2803
BsgI	GTGCAG	2 cut(s)	1115, 2793
Bsh1236I	CGCG	1 cut(s)	130
Bsh1285I	CGRYCG	1 cut(s)	119
BshFI	GGCC	3 cut(s)	1226, 3024, 3268
BshNI	GGYRCC	1 cut(s)	2529
BsiEI	CGRYCG	1 cut(s)	119
BsiHKAI	GWGCWC	1 cut(s)	2335
BsiSI	CCGG	5 cut(s)	236, 1424, 2669, 3315, 3350
BslFI	GGGAC	7 cut(s)	282, 748, 1579, 1831, 2705, 2786, 3050
BslI	CCNNNNNNNGG	7 cut(s)	248, 249, 912, 2179, 3031, 3073, 3121
BsmAI	GTCTC	9 cut(s)	638, 1646, 2032, 2247, 2406, 2907, 2940, 3010, 3047
BsmBI	CGTCTC	1 cut(s)	2247
BsmFI	GGGAC	7 cut(s)	282, 748, 1579, 1831, 2705, 2786, 3050
BsmI	GAATGC	3 cut(s)	1060, 1969, 3200
BsnI	GGCC	3 cut(s)	1226, 3024, 3268
Bso31I	GGTCTC	2 cut(s)	2907, 2940
Bsp119I	TTCGAA	2 cut(s)	656, 1019
Bsp1286I	GDGCHC	4 cut(s)	194, 243, 2335, 3079
Bsp13I	TCCGGA	1 cut(s)	3314
Bsp1407I	TGTACA	1 cut(s)	777
BspACI	CCGC	3 cut(s)	810, 939, 2324
BspANI	GGCC	3 cut(s)	1226, 3024, 3268
BspCNI	CTCAG	6 cut(s)	1771, 1834, 2389, 2934, 3228, 3262
BspEI	TCCGGA	1 cut(s)	3314
BspFNI	CGCG	1 cut(s)	130
BspLI	GGNNCC	8 cut(s)	240, 677, 904, 1428, 1587, 2531, 2971, 3313
BspOI	GCTAGC	1 cut(s)	1166
BspPI	GGATC	9 cut(s)	35, 53, 345, 514, 1056, 1421, 1434, 2106, 2190
BspQI	GCTCTTC	2 cut(s)	2407, 2641
BspT104I	TTCGAA	2 cut(s)	656, 1019
BspT107I	GGYRCC	1 cut(s)	2529
BspTNI	GGTCTC	2 cut(s)	2907, 2940
BsrBI	CCGCTC	1 cut(s)	810
BsrDI	GCAATG	4 cut(s)	451, 1411, 2932, 3097
BsrFI	RCCGGY	1 cut(s)	2668
BsrGI	TGTACA	1 cut(s)	777
BssAI	RCCGGY	1 cut(s)	2668
BssECI	CCNNGG	7 cut(s)	24, 1395, 1849, 2173, 2195, 2376, 3359
BssT1I	CCWWGG	2 cut(s)	1395, 3359
Bst2UI	CCWGG	5 cut(s)	1101, 1359, 1851, 2197, 2378
Bst6I	CTCTTC	3 cut(s)	706, 2407, 2641
BstAUI	TGTACA	1 cut(s)	777
BstBI	TTCGAA	2 cut(s)	656, 1019
BstC8I	GCNNGC	6 cut(s)	194, 1164, 2597, 3084, 3088, 3231
BstDEI	CTNAG	6 cut(s)	1779, 1842, 2397, 2942, 3215, 3270
BstDSI	CCRYGG	1 cut(s)	2173
BstFNI	CGCG	1 cut(s)	130
BstH2I	RGCGCY	2 cut(s)	166, 177
BstHHI	GCGC	7 cut(s)	132, 165, 176, 804, 3155, 3209, 3235
BstMAI	GTCTC	9 cut(s)	638, 1646, 2032, 2247, 2406, 2907, 2940, 3010, 3047
BstMCI	CGRYCG	1 cut(s)	119
BstNI	CCWGG	5 cut(s)	1101, 1359, 1851, 2197, 2378
BstNSI	RCATGY	3 cut(s)	205, 1062, 3090
BstPAI	GACNNNNGTC	1 cut(s)	2899
BstSCI	CCNGG	6 cut(s)	234, 1099, 1357, 1849, 2195, 2376
BstSFI	CTRYAG	2 cut(s)	2205, 2288
BstSLI	GKGCMC	3 cut(s)	194, 2335, 3079
BstUI	CGCG	1 cut(s)	130
BstV1I	GCAGC	7 cut(s)	16, 183, 647, 1108, 2970, 3038, 3247
BstV2I	GAAGAC	2 cut(s)	1402, 3228
BstX2I	RGATCY	2 cut(s)	1426, 2182
BstYI	RGATCY	2 cut(s)	1426, 2182
Bsu36I	CCTNAGG	1 cut(s)	3215
BsuRI	GGCC	3 cut(s)	1226, 3024, 3268
BtgI	CCRYGG	1 cut(s)	2173
BtgZI	GCGATG	4 cut(s)	111, 117, 1671, 3069
BtsI	GCAGTG	1 cut(s)	3044
BtsIMutI	CAGTG	7 cut(s)	574, 2064, 2166, 2776, 2939, 3044, 3283
Cac8I	GCNNGC	6 cut(s)	194, 1164, 2597, 3084, 3088, 3231
CaiI	CAGNNNCTG	1 cut(s)	1364
CfoI	GCGC	7 cut(s)	132, 165, 176, 804, 3155, 3209, 3235
Cfr10I	RCCGGY	1 cut(s)	2668
Cfr13I	GGNCC	5 cut(s)	1361, 1585, 1669, 3022, 3266
CseI	GACGC	2 cut(s)	2229, 2755
Csp6I	GTAC	5 cut(s)	157, 686, 778, 1683, 1941
CspCI	CAANNNNNGTGG	2 cut(s)	3155, 3190
CviQI	GTAC	5 cut(s)	157, 686, 778, 1683, 1941
DdeI	CTNAG	6 cut(s)	1779, 1842, 2397, 2942, 3215, 3270
DraI	TTTAAA	1 cut(s)	1572
EaeI	YGGCCR	1 cut(s)	1224
Eam1104I	CTCTTC	3 cut(s)	706, 2407, 2641
EarI	CTCTTC	3 cut(s)	706, 2407, 2641
EciI	GGCGGA	1 cut(s)	2313
Eco130I	CCWWGG	2 cut(s)	1395, 3359
Eco24I	GRGCYC	1 cut(s)	243
Eco31I	GGTCTC	2 cut(s)	2907, 2940
Eco32I	GATATC	2 cut(s)	1738, 2912
Eco47I	GGWCC	3 cut(s)	1361, 1585, 1669
Eco47III	AGCGCT	1 cut(s)	164
Eco57I	CTGAAG	3 cut(s)	711, 1023, 3163
Eco81I	CCTNAGG	1 cut(s)	3215
EcoO109I	RGGNCCY	1 cut(s)	1361
EcoRI	GAATTC	3 cut(s)	669, 1021, 1790
EcoRII	CCWGG	5 cut(s)	1099, 1357, 1849, 2195, 2376
EcoRV	GATATC	2 cut(s)	1738, 2912
EcoT14I	CCWWGG	2 cut(s)	1395, 3359
EcoT22I	ATGCAT	1 cut(s)	1060
EcoT38I	GRGCYC	1 cut(s)	243
ErhI	CCWWGG	2 cut(s)	1395, 3359
Esp3I	CGTCTC	1 cut(s)	2247
FalI	AAGNNNNNCTT	9 cut(s)	797, 829, 1379, 1411, 1725, 1757, 3161, 3193, 3400
FaqI	GGGAC	7 cut(s)	282, 748, 1579, 1831, 2705, 2786, 3050
FauI	CCCGC	1 cut(s)	932
FauNDI	CATATG	1 cut(s)	3047
FbaI	TGATCA	2 cut(s)	1531, 1597
FblI	GTMKAC	2 cut(s)	1922, 2287
Fnu4HI	GCNGC	8 cut(s)	30, 172, 636, 811, 1097, 2984, 3052, 3236
FriOI	GRGCYC	1 cut(s)	243
Fsp4HI	GCNGC	8 cut(s)	30, 172, 636, 811, 1097, 2984, 3052, 3236
FspBI	CTAG	9 cut(s)	423, 744, 963, 1163, 1167, 2370, 2409, 2501, 2918
FspI	TGCGCA	1 cut(s)	3154
GlaI	GCGC	7 cut(s)	131, 164, 175, 803, 3154, 3208, 3234
GluI	GCNGC	8 cut(s)	30, 172, 636, 811, 1097, 2984, 3052, 3236
GsaI	CCCAGC	3 cut(s)	2182, 2529, 2807
GsuI	CTGGAG	1 cut(s)	1341
HaeII	RGCGCY	2 cut(s)	166, 177
HaeIII	GGCC	3 cut(s)	1226, 3024, 3268
HapII	CCGG	5 cut(s)	236, 1424, 2669, 3315, 3350
HgaI	GACGC	2 cut(s)	2229, 2755
HhaI	GCGC	7 cut(s)	132, 165, 176, 804, 3155, 3209, 3235
Hin6I	GCGC	7 cut(s)	130, 163, 174, 802, 3153, 3207, 3233
HinP1I	GCGC	7 cut(s)	130, 163, 174, 802, 3153, 3207, 3233
HincII	GTYRAC	2 cut(s)	1147, 1923
HindII	GTYRAC	2 cut(s)	1147, 1923
HindIII	AAGCTT	1 cut(s)	494
HpaI	GTTAAC	1 cut(s)	1147
HpaII	CCGG	5 cut(s)	236, 1424, 2669, 3315, 3350
HphI	GGTGA	4 cut(s)	92, 101, 1258, 1333
Hpy166II	GTNNAC	7 cut(s)	835, 1147, 1266, 1863, 1923, 2288, 2333
Hpy8I	GTNNAC	7 cut(s)	835, 1147, 1266, 1863, 1923, 2288, 2333
Hpy99I	CGWCG	5 cut(s)	130, 514, 563, 2121, 2151
HpyCH4IV	ACGT	3 cut(s)	969, 2116, 3384
HpyF3I	CTNAG	6 cut(s)	1779, 1842, 2397, 2942, 3215, 3270
HpySE526I	ACGT	3 cut(s)	969, 2116, 3384
HspAI	GCGC	7 cut(s)	130, 163, 174, 802, 3153, 3207, 3233
Kpn2I	TCCGGA	1 cut(s)	3314
Ksp22I	TGATCA	2 cut(s)	1531, 1597
KspAI	GTTAAC	1 cut(s)	1147
LguI	GCTCTTC	2 cut(s)	2407, 2641
LmnI	GCTCC	7 cut(s)	238, 461, 1360, 1853, 2378, 2969, 3034
Lsp1109I	GCAGC	7 cut(s)	16, 183, 647, 1108, 2970, 3038, 3247
LweI	GCATC	5 cut(s)	1094, 1629, 1939, 2487, 3164
MaeI	CTAG	9 cut(s)	423, 744, 963, 1163, 1167, 2370, 2409, 2501, 2918
MaeII	ACGT	3 cut(s)	969, 2116, 3384
MaeIII	GTNAC	3 cut(s)	80, 413, 1877
MbiI	CCGCTC	1 cut(s)	810
MflI	RGATCY	2 cut(s)	1426, 2182
MhlI	GDGCHC	4 cut(s)	194, 243, 2335, 3079
MlsI	TGGCCA	1 cut(s)	1226
MluNI	TGGCCA	1 cut(s)	1226
MlyI	GAGTC	5 cut(s)	158, 1829, 1853, 2408, 3072
MmeI	TCCRAC	1 cut(s)	411
Mox20I	TGGCCA	1 cut(s)	1226
Mph1103I	ATGCAT	1 cut(s)	1060
MroI	TCCGGA	1 cut(s)	3314
MroXI	GAANNNNTTC	1 cut(s)	669
MscI	TGGCCA	1 cut(s)	1226
MslI	CAYNNNNRTG	6 cut(s)	1220, 2338, 2544, 2587, 2649, 3392
Msp20I	TGGCCA	1 cut(s)	1226
MspI	CCGG	5 cut(s)	236, 1424, 2669, 3315, 3350
MspR9I	CCNGG	6 cut(s)	236, 1101, 1359, 1851, 2197, 2378
Mva1269I	GAATGC	3 cut(s)	1060, 1969, 3200
MvaI	CCWGG	5 cut(s)	1101, 1359, 1851, 2197, 2378
MvnI	CGCG	1 cut(s)	130
NciI	CCSGG	1 cut(s)	236
NdeI	CATATG	1 cut(s)	3047
NheI	GCTAGC	1 cut(s)	1162
NlaIV	GGNNCC	8 cut(s)	240, 677, 904, 1428, 1587, 2531, 2971, 3313
NmeAIII	GCCGAG	3 cut(s)	49, 222, 2352
NmuCI	GTSAC	1 cut(s)	80
NsbI	TGCGCA	1 cut(s)	3154
NsiI	ATGCAT	1 cut(s)	1060
NspI	RCATGY	3 cut(s)	205, 1062, 3090
NspV	TTCGAA	2 cut(s)	656, 1019
PaeI	GCATGC	1 cut(s)	3090
PciSI	GCTCTTC	2 cut(s)	2407, 2641
PcsI	WCGNNNNNNNCGW	3 cut(s)	150, 2122, 3381
PctI	GAATGC	3 cut(s)	1060, 1969, 3200
PdmI	GAANNNNTTC	1 cut(s)	669
PflFI	GACNNNGTC	1 cut(s)	3383
PfoI	TCCNGGA	1 cut(s)	234
PkrI	GCNGC	8 cut(s)	31, 173, 637, 812, 1098, 2985, 3053, 3237
Ple19I	CGATCG	1 cut(s)	119
PleI	GAGTC	5 cut(s)	157, 1829, 1852, 2407, 3071
PpsI	GAGTC	5 cut(s)	157, 1829, 1852, 2407, 3071
PpuMI	RGGWCCY	1 cut(s)	1361
PshAI	GACNNNNGTC	1 cut(s)	2899
PshBI	ATTAAT	1 cut(s)	6
PsiI	TTATAA	1 cut(s)	3421
Psp5II	RGGWCCY	1 cut(s)	1361
Psp6I	CCWGG	5 cut(s)	1099, 1357, 1849, 2195, 2376
PspFI	CCCAGC	3 cut(s)	2178, 2525, 2803
PspGI	CCWGG	5 cut(s)	1099, 1357, 1849, 2195, 2376
PspN4I	GGNNCC	8 cut(s)	240, 677, 904, 1428, 1587, 2531, 2971, 3313
PspPI	GGNCC	5 cut(s)	1361, 1585, 1669, 3022, 3266
PspPPI	RGGWCCY	1 cut(s)	1361
PstNI	CAGNNNCTG	1 cut(s)	1364
PsuI	RGATCY	2 cut(s)	1426, 2182
PsyI	GACNNNGTC	1 cut(s)	3383
PvuI	CGATCG	1 cut(s)	119
RsaI	GTAC	5 cut(s)	158, 687, 779, 1684, 1942
RsaNI	GTAC	5 cut(s)	157, 686, 778, 1683, 1941
RseI	CAYNNNNRTG	6 cut(s)	1220, 2338, 2544, 2587, 2649, 3392
SalI	GTCGAC	1 cut(s)	1921
SapI	GCTCTTC	2 cut(s)	2407, 2641
SatI	GCNGC	8 cut(s)	30, 172, 636, 811, 1097, 2984, 3052, 3236
Sau96I	GGNCC	5 cut(s)	1361, 1585, 1669, 3022, 3266
SchI	GAGTC	5 cut(s)	158, 1829, 1853, 2408, 3072
ScrFI	CCNGG	6 cut(s)	236, 1101, 1359, 1851, 2197, 2378
SduI	GDGCHC	4 cut(s)	194, 243, 2335, 3079
SfaNI	GCATC	5 cut(s)	1094, 1629, 1939, 2487, 3164
SfcI	CTRYAG	2 cut(s)	2205, 2288
SfuI	TTCGAA	2 cut(s)	656, 1019
SinI	GGWCC	3 cut(s)	1361, 1585, 1669
SmiMI	CAYNNNNRTG	6 cut(s)	1220, 2338, 2544, 2587, 2649, 3392
SmlI	CTYRAG	4 cut(s)	497, 1664, 2355, 3007
SmoI	CTYRAG	4 cut(s)	497, 1664, 2355, 3007
SphI	GCATGC	1 cut(s)	3090
SsiI	CCGC	3 cut(s)	810, 939, 2324
SspI	AATATT	4 cut(s)	391, 1417, 2431, 2513
SspMI	CTAG	9 cut(s)	423, 744, 963, 1163, 1167, 2370, 2409, 2501, 2918
StyD4I	CCNGG	6 cut(s)	234, 1099, 1357, 1849, 2195, 2376
StyI	CCWWGG	2 cut(s)	1395, 3359
TaiI	ACGT	3 cut(s)	972, 2119, 3387
TatI	WGTACW	2 cut(s)	777, 1682
TauI	GCSGC	1 cut(s)	813
TscAI	CASTG	7 cut(s)	574, 2071, 2166, 2776, 2946, 3044, 3283
TseFI	GTSAC	1 cut(s)	80
TseI	GCWGC	7 cut(s)	29, 171, 635, 1096, 2983, 3051, 3235
Tsp45I	GTSAC	1 cut(s)	80
TspRI	CASTG	7 cut(s)	574, 2071, 2166, 2776, 2946, 3044, 3283
Tth111I	GACNNNGTC	1 cut(s)	3383
VneI	GTGCAC	1 cut(s)	2331
VpaK11BI	GGWCC	3 cut(s)	1361, 1585, 1669
VspI	ATTAAT	1 cut(s)	6
XapI	RAATTY	9 cut(s)	245, 669, 701, 1014, 1021, 1790, 1824, 2469, 3015
XbaI	TCTAGA	2 cut(s)	743, 2917
XceI	RCATGY	3 cut(s)	205, 1062, 3090
XcmI	CCANNNNNNNNNTGG	1 cut(s)	184
XmiI	GTMKAC	2 cut(s)	1922, 2287
XmnI	GAANNNNTTC	1 cut(s)	669
XspI	CTAG	9 cut(s)	423, 744, 963, 1163, 1167, 2370, 2409, 2501, 2918
Zsp2I	ATGCAT	1 cut(s)	1060

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.