Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0010875

Physical Location & Seq

Genomic Coordinates Forward (+)

27111374 .. 27116427

Gene Structure 5054 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1178400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3414 bp

ATGATTGATGGAGAGACGACTGCCGGGGCCTCCTCTCCGGATCCCTCAATCCCGCTTGATCCCAAATCGGACGGTGTTGGTGACGGCGGCAATTTCGATGGCGATGTTTCGCCCCGATCGCCTCCGTCGCGCTACTCCTCGTTCGGGGAGTCCGAGTACGAGCGCTATTGTAGCGCCAACTCGATGATGGGCAAGCCGAGCATGTGTAGCGCAATCACGGTTTTCAATGATTTCCCGGAGCCCAAATTCGGGTCGTTGAAAAGTTGGAGACTTGGGGAGGAGAGTGGTGGTTTGGATAATTTTAGTTTGGGAGGGAGGATTGAGAGGAATCGAGAGGATCGGAGAGTTTTGAGTAGTGGTAGGATTGAGTTTGGGAAGGAGGATGGTAGTATTGGAGGACGGCGGACTGCGAATTACGGGTCTAGTGGGTCGGAATTGTATGGCAATGAGGATGCTGGTGGAGCTCATGATGTGGATGAATTGATGTCTTGGAAGCTTGAGAGTGGATCGTCGGGTTTAAGGGGGGGTTTGGATGTGAAGTATGGTTCGGATAATAGTGACGAGGACAGTGAAAAAGGTACGGAAGTTTGGAGGGGAGTTGTGGGAAGTGATAGTGTTGGAGCTGGTGTAGCTGCCCAAGAGACCAATGATTCGAAAGGGGTTGGGATTGGGAACCAGTTTGTACCCAAAGTTGAAGAATTAGATGGAGGAGAGATAGGTAGGGAGGAAGGGACCTCTAATGAGTATTCAGAGGAAGAGGGGTCCATGTACAACTATGGTTCGGATGATGAGCGCAAGAGTGGATTTAGTCAGCAGAGGAATGTCCACCATTATCAGCAAGAAAAACCCCAGAATGAAAATCCGTTTCTTATTAACACATCTGTAGCCTTTGGCTCCGATGATTGGGATGATTTCATGGAAGAGACTGGAGGAAGTAATTTAGATTCTTTCACGAACATATTTGAGGACCGGAGAGGAGAGAAAGTTGAAACCAAAAGGAAAGTTTCGAATTCCACTTCCATCACTTCAGTTGAGCATCAGAATGCATGTCAAACTGAGCAAGGAAATGACTTGACAGATGTGCAGCCTGGATGCAAACAAGTTCAAGCTGATAGTAAATCGGTAGAAGATGTTAACAGTTCCATGAAGCTAGCTAGTTCTCCAAGTTTTCTTGAAACCGATAGAGTAGAAGATGTGAAGGACATTCCTATGGCCAGTTATCAAGTTCAAGCTGTTGCTGATTTGGTTGAGTTTACCAAAAGTTCTTGTACTACCCCGACTGGTTTTCAAAATGTTCAAGAACCAGGGCTAGAAGATTCAAGTGATATTCCTTTGACAAATAATCAAGTTCCAGGTTCTGATGAATCAGCAAAACATAATAAAGATTCCTTGGTTGGCAATGTCCTTGAACTTCAGCCGGATCCACAAGCAAAAGAAATTCCTGACAAAAAGGGTTTGAGCATTTTGGATAATGGTGTTTCTGATGTGCATACATATATGAATACCGGGGAGGTCCTTGGGACTGATCATGGCCAAGATTTAGAACAGAAAAAGTTGGGGACTTTAAAAGTTAAATTGGACCCCCTTTCGAATATCTCAACTAATCAAATTAGCATCTATTCAACCAGAACTTCTGGAAATATGAAGACAGAGTTCCTTGAGGACCATAAGCCAAGTACACTGCCATCAACTTTTGAAAATAATACCACGAAAAGTCCTGTTTCAGAAGATATCCTTGAAGAATATCCTATGCCAGTTAAGACGGATAATTTTGAGCTTAATGAATTCTATGATGAGTTTGTTAATGAAATGGAGGAAATTCTGCTCGACTCTGCTGAGTCCCCAGGGGCAAGGTTCACTCATAGCAACAGGTTCTTACAATCACAGCAATATCTACCAGTAAGAGATGGTGGGTCGACTGCTTCTACTTCTGGTACAGATGATGCTCATTTGTTCAATCAGCATTCGTTGAGAATTGATGGGGTCGAAGTGGTGGGTGCAAGGCAGAAGAAGGGAGATGTCTCATTTAGTGAAAGACTGGTGGGAGTTAAAGAATACACTGTGTATAAAATAAAAGTATTGAGTGGCGAGGATCAATGGGAGGTGGAACGTCGATATCGCGATTTCTTTACACTCTATCGTCGATTAAAAACATTTTTTTCTGACCACGGCTGGGATCTACCTTCTCCCTGGTCTGCTGTAGAAAAGGAATCCAGAAAGATTTTTGGAAATGCGTCTCCTGATGTTATTGCAGAGAGAAGTGTTCTCATCCAAGAATGTCTACGGTCCGTTCTCCATTACAGGTTCTTTTCCAGTCCGCCAAGTGCATTAGTATGGTTTCTCTCTCCTCAAGATTCAGTTCCTAGCTCCCTGGAATCATATACACCTGAGTCTCTAACTAGAAGAGCAGACACAGAAGATATATCCACTTTGGGAAAGACCATATCACTTATTGTTGAAATTCGACAATCCAAATCTTTGAAGCAGATGCTAGAAGCACAACACTATACTTGTGCTGGATGCCACAAGCATTTTGATGATGGAAGGACTTTGATACAGGATTTTGCACAGACACTAGGATGGGGCAAGCCTCGACTTTGTGAATATACTGGTCAATTATTTTGCTCTTCGTGCCATGCAAATGAAATTGCAATCATACCAGCAAGAGTGTTGCATAACTGGGATTTTACGCAATATCCTGTTTCGCAATATGCTAAATCTTATCTGGATTCTATACATGACCAGCCCATGCTCTGCGTCAGTGCAGTTAATCCATTCCTTTTCTCGAAGGTTCCAGCATTGCTTAATGTTATGGGTGTGAGGAAGAAAATAGGAACCATTCTTCCTTATGTTCGTTGCCCATTCCGTAAGTCAATCAACAAAGGATTCGGGTCTCGAAGATATCTTCTAGAAAGCAATGATTTTTTTGCACTGAGAGACCTCATTGATCTTTCCAAAGGAGCCTTTGCAGTGCTGCCCGTGATTGTGGAGACTGCCTTGAGGAAAATTCTGGACCATATAACGGAGCAGTGTCTCATATGCTGTGATGTGGGAGTCCCCTGTGGAGCACGACAAGCATGCAGTGATCCTTCATCTCTCATTTTCCCCTTTCAGGAGGATGAAATTGAAAGGTGTCCGTCTTGCGAATCAGTATTCCACAAGCCTTGCTTAAGAAAGGTCATGGACTGCACTTGCGGGGCACGCCTCAGGGAAGACGAACCAGCACAGCTTATCAAGAGGGCAAGCTCCGGGGTCAGTGCTGAGATCAGTGGACTTCTGGACTTGTTTGGGGGAGGGTCAGGTTCCGGATTACTCTCGGGTCTATTTTCGAAGGTGAAGCCAGAGAAGCCGAGGGAACATAAAGACAGCGACAACGTCATTTTAATGGGTTCGTTACCAAGCACTTCTTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0009987 GO:0012505 GO:0030154 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045445 GO:0048856 GO:0048869 GO:0061061

KEGG Pathways

Metabolic & Signaling

ko04140 map04140

Pfam Domains

Protein Families

PX zf-RING_9

Protein Analysis

1138

Amino Acids

125.37

Weight (kDa)

4.86

Isoelectric Point (pI)

50.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PX	PF00787	695 - 775	5.1e-06	PX domain
RH_dom	PF13901	865 - 1070	1.1e-54	Rubicon Homology Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010875)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48195
fragaria_vesca	FvH4_5g05470 FvH4_5g05470 FvH4_5g05470
malus_domestica	MD06G1172500.v1.1 MD14G1178400.v1.1
prunus_persica	Prupe.5G171800_v2.0.a1 Prupe.5G171800_v2.0.a1
pyrus_communis	pycom06g15360 pycom14g14870
rosa_chinensis	RchiOBHm_Chr7g0193371
rosa_laevigata	RLG00000004275
rosa_multiflora	Rmu_co8475531.1_g000001
rosa_roxburghii	Rroxscaffold_3G00262170
rosa_rugosa	Rorug07G0009200
rosa_samantha	Rh7AG135500 Rh7BG135800 Rh7CG139200 Rh7DG137200
rosa_wichuraiana	Rw7G011550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3412
AccI	GTMKAC	2 cut(s)	1916, 2281
AccII	CGCG	2 cut(s)	130, 2121
AccIII	TCCGGA	2 cut(s)	37, 3305
AciI	CCGC	5 cut(s)	53, 87, 403, 2318, 3195
AcoI	YGGCCR	2 cut(s)	1212, 1531
AcsI	RAATTY	7 cut(s)	245, 1009, 1437, 1784, 1818, 2460, 3006
AcuI	CTGAAG	2 cut(s)	1011, 1397
AfaI	GTAC	7 cut(s)	158, 580, 684, 770, 1270, 1678, 1936
AfeI	AGCGCT	1 cut(s)	164
AfiI	CCNNNNNNNGG	7 cut(s)	144, 248, 249, 903, 2173, 3022, 3112
AflII	CTTAAG	1 cut(s)	3169
AjnI	CCWGG	6 cut(s)	1087, 1303, 1351, 1843, 2189, 2370
AloI	GAACNNNNNNTCC	2 cut(s)	2827, 2859
Alw21I	GWGCWC	2 cut(s)	466, 3070
AlwNI	CAGNNNCTG	1 cut(s)	1358
Ama87I	CYCGRG	1 cut(s)	3316
Aor13HI	TCCGGA	2 cut(s)	37, 3305
Aor51HI	AGCGCT	1 cut(s)	164
AoxI	GGCC	3 cut(s)	27, 1212, 1531
ApeKI	GCWGC	3 cut(s)	632, 1084, 2974
ApoI	RAATTY	7 cut(s)	245, 1009, 1437, 1784, 1818, 2460, 3006
ArsI	GACNNNNNNTTYG	4 cut(s)	404, 436, 634, 666
AspLEI	GCGC	5 cut(s)	132, 165, 176, 212, 795
AspS9I	GGNCC	9 cut(s)	27, 732, 762, 967, 1513, 1579, 1663, 2286, 3013
AsuC2I	CCSGG	4 cut(s)	25, 236, 1507, 3250
AsuHPI	GGTGA	2 cut(s)	92, 3346
AsuII	TTCGAA	4 cut(s)	653, 1007, 1589, 3329
AsuNHI	GCTAGC	1 cut(s)	1150
AvaI	CYCGRG	1 cut(s)	3316
AvaII	GGWCC	8 cut(s)	732, 762, 967, 1513, 1579, 1663, 2286, 3013
AxyI	CCTNAGG	1 cut(s)	3206
BaeGI	GKGCMC	1 cut(s)	3202
BaeI	ACNNNNGTAYC	2 cut(s)	570, 603
BalI	TGGCCA	2 cut(s)	1214, 1533
BamHI	GGATCC	2 cut(s)	40, 1420
BanII	GRGCYC	2 cut(s)	243, 466
BbsI	GAAGAC	2 cut(s)	1652, 3219
Bbv12I	GWGCWC	2 cut(s)	466, 3070
BbvI	GCAGC	3 cut(s)	619, 1096, 2961
BceAI	ACGGC	3 cut(s)	100, 416, 2185
BcgI	CGANNNNNNTGC	2 cut(s)	3060, 3094
BciT130I	CCWGG	6 cut(s)	1089, 1305, 1353, 1845, 2191, 2372
BclI	TGATCA	1 cut(s)	1525
BcnI	CCSGG	4 cut(s)	25, 236, 1507, 3250
BfaI	CTAG	9 cut(s)	423, 1151, 1155, 1310, 2364, 2400, 2492, 2576, 2909
BfmI	CTRYAG	2 cut(s)	882, 2199
BfoI	RGCGCY	2 cut(s)	166, 177
BfrI	CTTAAG	1 cut(s)	3169
BisI	GCNGC	4 cut(s)	88, 633, 1085, 2975
BlsI	GCNGC	4 cut(s)	89, 634, 1086, 2976
Bme18I	GGWCC	8 cut(s)	732, 762, 967, 1513, 1579, 1663, 2286, 3013
BmeT110I	CYCGRG	1 cut(s)	3316
BmgT120I	GGNCC	9 cut(s)	27, 732, 762, 967, 1513, 1579, 1663, 2286, 3013
BmrI	ACTGGG	1 cut(s)	2689
BmsI	GCATC	7 cut(s)	442, 1045, 1082, 1623, 1933, 2478, 2510
BmtI	GCTAGC	1 cut(s)	1154
BmuI	ACTGGG	1 cut(s)	2689
BpiI	GAAGAC	2 cut(s)	1652, 3219
BpmI	CTGGAG	1 cut(s)	948
Bpu14I	TTCGAA	4 cut(s)	653, 1007, 1589, 3329
BpuEI	CTTGAG	4 cut(s)	518, 1679, 2334, 3019
BpuMI	CCSGG	4 cut(s)	25, 236, 1507, 3250
BsaI	GGTCTC	3 cut(s)	635, 2898, 2931
BsaWI	WCCGGW	3 cut(s)	37, 969, 3305
BsaXI	ACNNNNNCTCC	6 cut(s)	140, 170, 612, 642, 969, 999
Bsc4I	CCNNNNNNNGG	7 cut(s)	144, 248, 249, 903, 2173, 3022, 3112
Bse21I	CCTNAGG	1 cut(s)	3206
Bse3DI	GCAATG	4 cut(s)	451, 1405, 2798, 2923
BseAI	TCCGGA	2 cut(s)	37, 3305
BseBI	CCWGG	6 cut(s)	1089, 1305, 1353, 1845, 2191, 2372
BseLI	CCNNNNNNNGG	7 cut(s)	144, 248, 249, 903, 2173, 3022, 3112
BseMI	GCAATG	4 cut(s)	451, 1405, 2798, 2923
BseMII	CTCAG	6 cut(s)	1047, 1827, 2379, 2924, 3220, 3252
BseRI	GAGGAG	6 cut(s)	22, 127, 293, 723, 990, 2337
BseSI	GKGCMC	1 cut(s)	3202
BseXI	GCAGC	3 cut(s)	619, 1096, 2961
BseYI	CCCAGC	1 cut(s)	2172
BsgI	GTGCAG	3 cut(s)	1103, 2784, 3172
Bsh1236I	CGCG	2 cut(s)	130, 2121
Bsh1285I	CGRYCG	1 cut(s)	119
BshFI	GGCC	3 cut(s)	29, 1214, 1533
BsiEI	CGRYCG	1 cut(s)	119
BsiHKAI	GWGCWC	2 cut(s)	466, 3070
BsiHKCI	CYCGRG	1 cut(s)	3316
BsiSI	CCGG	8 cut(s)	24, 38, 236, 970, 1418, 1506, 3249, 3306
BslFI	GGGAC	5 cut(s)	745, 1534, 1573, 1825, 3041
BslI	CCNNNNNNNGG	7 cut(s)	144, 248, 249, 903, 2173, 3022, 3112
BsmBI	CGTCTC	2 cut(s)	8, 2241
BsmFI	GGGAC	5 cut(s)	745, 1534, 1573, 1825, 3041
BsmI	GAATGC	2 cut(s)	1048, 1963
BsnI	GGCC	3 cut(s)	29, 1214, 1533
Bso31I	GGTCTC	3 cut(s)	635, 2898, 2931
BsoBI	CYCGRG	1 cut(s)	3316
Bsp119I	TTCGAA	4 cut(s)	653, 1007, 1589, 3329
Bsp1286I	GDGCHC	4 cut(s)	243, 466, 3070, 3202
Bsp13I	TCCGGA	2 cut(s)	37, 3305
Bsp1407I	TGTACA	1 cut(s)	768
Bsp68I	TCGCGA	1 cut(s)	2121
BspACI	CCGC	5 cut(s)	53, 87, 403, 2318, 3195
BspANI	GGCC	3 cut(s)	29, 1214, 1533
BspCNI	CTCAG	6 cut(s)	1048, 1828, 2380, 2925, 3219, 3253
BspEI	TCCGGA	2 cut(s)	37, 3305
BspFNI	CGCG	2 cut(s)	130, 2121
BspHI	TCATGA	1 cut(s)	466
BspOI	GCTAGC	1 cut(s)	1154
BspQI	GCTCTTC	2 cut(s)	2398, 2632
BspT104I	TTCGAA	4 cut(s)	653, 1007, 1589, 3329
BspTI	CTTAAG	1 cut(s)	3169
BspTNI	GGTCTC	3 cut(s)	635, 2898, 2931
BsrDI	GCAATG	4 cut(s)	451, 1405, 2798, 2923
BsrGI	TGTACA	1 cut(s)	768
BssT1I	CCWWGG	2 cut(s)	1389, 1516
Bst2UI	CCWGG	6 cut(s)	1089, 1305, 1353, 1845, 2191, 2372
Bst4CI	ACNGT	6 cut(s)	74, 220, 569, 1139, 2062, 2286
Bst6I	CTCTTC	4 cut(s)	750, 915, 2398, 2632
BstAFI	CTTAAG	1 cut(s)	3169
BstAUI	TGTACA	1 cut(s)	768
BstBI	TTCGAA	4 cut(s)	653, 1007, 1589, 3329
BstC8I	GCNNGC	6 cut(s)	194, 1152, 2588, 3079, 3202, 3244
BstDEI	CTNAG	6 cut(s)	1056, 1836, 2388, 2933, 3206, 3261
BstDSI	CCRYGG	1 cut(s)	2167
BstFNI	CGCG	2 cut(s)	130, 2121
BstH2I	RGCGCY	2 cut(s)	166, 177
BstHHI	GCGC	5 cut(s)	132, 165, 176, 212, 795
BstMCI	CGRYCG	1 cut(s)	119
BstNI	CCWGG	6 cut(s)	1089, 1305, 1353, 1845, 2191, 2372
BstNSI	RCATGY	3 cut(s)	205, 1050, 3081
BstSFI	CTRYAG	2 cut(s)	882, 2199
BstSLI	GKGCMC	1 cut(s)	3202
BstUI	CGCG	2 cut(s)	130, 2121
BstV1I	GCAGC	3 cut(s)	619, 1096, 2961
BstV2I	GAAGAC	2 cut(s)	1652, 3219
BstX2I	RGATCY	3 cut(s)	40, 1420, 2176
BstYI	RGATCY	3 cut(s)	40, 1420, 2176
Bsu36I	CCTNAGG	1 cut(s)	3206
BsuRI	GGCC	3 cut(s)	29, 1214, 1533
BtgI	CCRYGG	1 cut(s)	2167
BtgZI	GCGATG	1 cut(s)	117
BtsI	GCAGTG	4 cut(s)	1679, 2976, 3035, 3088
BtuMI	TCGCGA	1 cut(s)	2121
Cac8I	GCNNGC	6 cut(s)	194, 1152, 2588, 3079, 3202, 3244
CaiI	CAGNNNCTG	1 cut(s)	1358
CciI	TCATGA	1 cut(s)	466
CfoI	GCGC	5 cut(s)	132, 165, 176, 212, 795
Cfr13I	GGNCC	9 cut(s)	27, 732, 762, 967, 1513, 1579, 1663, 2286, 3013
CpoI	CGGWCCG	1 cut(s)	2286
CseI	GACGC	2 cut(s)	2223, 2746
Csp6I	GTAC	7 cut(s)	157, 579, 683, 769, 1269, 1677, 1935
CspCI	CAANNNNNGTGG	2 cut(s)	3146, 3181
CspI	CGGWCCG	1 cut(s)	2286
CviQI	GTAC	7 cut(s)	157, 579, 683, 769, 1269, 1677, 1935
DdeI	CTNAG	6 cut(s)	1056, 1836, 2388, 2933, 3206, 3261
DraI	TTTAAA	1 cut(s)	1566
EaeI	YGGCCR	2 cut(s)	1212, 1531
Eam1104I	CTCTTC	4 cut(s)	750, 915, 2398, 2632
EarI	CTCTTC	4 cut(s)	750, 915, 2398, 2632
EciI	GGCGGA	2 cut(s)	418, 2307
Ecl136II	GAGCTC	1 cut(s)	464
Eco130I	CCWWGG	2 cut(s)	1389, 1516
Eco24I	GRGCYC	2 cut(s)	243, 466
Eco31I	GGTCTC	3 cut(s)	635, 2898, 2931
Eco32I	GATATC	3 cut(s)	1732, 2117, 2903
Eco47I	GGWCC	8 cut(s)	732, 762, 967, 1513, 1579, 1663, 2286, 3013
Eco47III	AGCGCT	1 cut(s)	164
Eco53kI	GAGCTC	1 cut(s)	464
Eco57I	CTGAAG	2 cut(s)	1011, 1397
Eco81I	CCTNAGG	1 cut(s)	3206
Eco88I	CYCGRG	1 cut(s)	3316
EcoICRI	GAGCTC	1 cut(s)	464
EcoO109I	RGGNCCY	3 cut(s)	27, 732, 1513
EcoRI	GAATTC	2 cut(s)	1009, 1784
EcoRII	CCWGG	6 cut(s)	1087, 1303, 1351, 1843, 2189, 2370
EcoRV	GATATC	3 cut(s)	1732, 2117, 2903
EcoT14I	CCWWGG	2 cut(s)	1389, 1516
EcoT22I	ATGCAT	1 cut(s)	1048
EcoT38I	GRGCYC	2 cut(s)	243, 466
ErhI	CCWWGG	2 cut(s)	1389, 1516
Esp3I	CGTCTC	2 cut(s)	8, 2241
FalI	AAGNNNNNCTT	5 cut(s)	1719, 1751, 3152, 3184, 3391
FaqI	GGGAC	5 cut(s)	745, 1534, 1573, 1825, 3041
FauI	CCCGC	2 cut(s)	60, 3188
FauNDI	CATATG	1 cut(s)	3038
FbaI	TGATCA	1 cut(s)	1525
FblI	GTMKAC	2 cut(s)	1916, 2281
Fnu4HI	GCNGC	4 cut(s)	88, 633, 1085, 2975
FriOI	GRGCYC	2 cut(s)	243, 466
Fsp4HI	GCNGC	4 cut(s)	88, 633, 1085, 2975
FspBI	CTAG	9 cut(s)	423, 1151, 1155, 1310, 2364, 2400, 2492, 2576, 2909
GlaI	GCGC	5 cut(s)	131, 164, 175, 211, 794
GluI	GCNGC	4 cut(s)	88, 633, 1085, 2975
GsaI	CCCAGC	1 cut(s)	2176
GsuI	CTGGAG	1 cut(s)	948
HaeII	RGCGCY	2 cut(s)	166, 177
HaeIII	GGCC	3 cut(s)	29, 1214, 1533
HapII	CCGG	8 cut(s)	24, 38, 236, 970, 1418, 1506, 3249, 3306
HgaI	GACGC	2 cut(s)	2223, 2746
HhaI	GCGC	5 cut(s)	132, 165, 176, 212, 795
Hin6I	GCGC	5 cut(s)	130, 163, 174, 210, 793
HinP1I	GCGC	5 cut(s)	130, 163, 174, 210, 793
HincII	GTYRAC	2 cut(s)	1135, 1917
HindII	GTYRAC	2 cut(s)	1135, 1917
HindIII	AAGCTT	1 cut(s)	494
HpaI	GTTAAC	1 cut(s)	1135
HpaII	CCGG	8 cut(s)	24, 38, 236, 970, 1418, 1506, 3249, 3306
HphI	GGTGA	2 cut(s)	92, 3346
Hpy166II	GTNNAC	8 cut(s)	826, 1135, 1254, 1679, 1857, 1917, 2282, 3272
Hpy8I	GTNNAC	8 cut(s)	826, 1135, 1254, 1679, 1857, 1917, 2282, 3272
Hpy99I	CGWCG	4 cut(s)	130, 514, 2115, 2145
HpyAV	CCTTC	9 cut(s)	370, 722, 1192, 2005, 2193, 2538, 2782, 3099, 3325
HpyCH4III	ACNGT	6 cut(s)	74, 220, 569, 1139, 2062, 2286
HpyCH4IV	ACGT	2 cut(s)	2110, 3375
HpyF3I	CTNAG	6 cut(s)	1056, 1836, 2388, 2933, 3206, 3261
HpySE526I	ACGT	2 cut(s)	2110, 3375
HspAI	GCGC	5 cut(s)	130, 163, 174, 210, 793
Kpn2I	TCCGGA	2 cut(s)	37, 3305
Ksp22I	TGATCA	1 cut(s)	1525
KspAI	GTTAAC	1 cut(s)	1135
LguI	GCTCTTC	2 cut(s)	2398, 2632
LmnI	GCTCC	9 cut(s)	238, 461, 620, 899, 2372, 2960, 3025, 3065, 3251
Lsp1109I	GCAGC	3 cut(s)	619, 1096, 2961
LweI	GCATC	7 cut(s)	442, 1045, 1082, 1623, 1933, 2478, 2510
MaeI	CTAG	9 cut(s)	423, 1151, 1155, 1310, 2364, 2400, 2492, 2576, 2909
MaeII	ACGT	2 cut(s)	2110, 3375
MaeIII	GTNAC	3 cut(s)	80, 557, 3393
MflI	RGATCY	3 cut(s)	40, 1420, 2176
MhlI	GDGCHC	4 cut(s)	243, 466, 3070, 3202
MlsI	TGGCCA	2 cut(s)	1214, 1533
MluNI	TGGCCA	2 cut(s)	1214, 1533
MlyI	GAGTC	5 cut(s)	158, 1823, 1847, 2399, 3063
MmeI	TCCRAC	3 cut(s)	245, 411, 598
Mox20I	TGGCCA	2 cut(s)	1214, 1533
Mph1103I	ATGCAT	1 cut(s)	1048
MroI	TCCGGA	2 cut(s)	37, 3305
MscI	TGGCCA	2 cut(s)	1214, 1533
MslI	CAYNNNNRTG	7 cut(s)	1041, 1208, 2332, 2535, 2578, 2640, 3383
Msp20I	TGGCCA	2 cut(s)	1214, 1533
MspCI	CTTAAG	1 cut(s)	3169
MspI	CCGG	8 cut(s)	24, 38, 236, 970, 1418, 1506, 3249, 3306
Mva1269I	GAATGC	2 cut(s)	1048, 1963
MvaI	CCWGG	6 cut(s)	1089, 1305, 1353, 1845, 2191, 2372
MvnI	CGCG	2 cut(s)	130, 2121
NciI	CCSGG	4 cut(s)	25, 236, 1507, 3250
NdeI	CATATG	1 cut(s)	3038
NheI	GCTAGC	1 cut(s)	1150
NmeAIII	GCCGAG	2 cut(s)	222, 3375
NmuCI	GTSAC	2 cut(s)	80, 557
NruI	TCGCGA	1 cut(s)	2121
NsiI	ATGCAT	1 cut(s)	1048
NspI	RCATGY	3 cut(s)	205, 1050, 3081
NspV	TTCGAA	4 cut(s)	653, 1007, 1589, 3329
PaeI	GCATGC	1 cut(s)	3081
PagI	TCATGA	1 cut(s)	466
PasI	CCCWGGG	1 cut(s)	1844
PciSI	GCTCTTC	2 cut(s)	2398, 2632
PcsI	WCGNNNNNNNCGW	1 cut(s)	150
PctI	GAATGC	2 cut(s)	1048, 1963
PflFI	GACNNNGTC	1 cut(s)	3374
PfoI	TCCNGGA	1 cut(s)	234
PkrI	GCNGC	4 cut(s)	89, 634, 1086, 2976
Ple19I	CGATCG	1 cut(s)	119
PleI	GAGTC	5 cut(s)	157, 1823, 1846, 2398, 3062
PpsI	GAGTC	5 cut(s)	157, 1823, 1846, 2398, 3062
PpuMI	RGGWCCY	2 cut(s)	732, 1513
PsiI	TTATAA	1 cut(s)	3412
Psp124BI	GAGCTC	1 cut(s)	466
Psp5II	RGGWCCY	2 cut(s)	732, 1513
Psp6I	CCWGG	6 cut(s)	1087, 1303, 1351, 1843, 2189, 2370
PspFI	CCCAGC	1 cut(s)	2172
PspGI	CCWGG	6 cut(s)	1087, 1303, 1351, 1843, 2189, 2370
PspPI	GGNCC	9 cut(s)	27, 732, 762, 967, 1513, 1579, 1663, 2286, 3013
PspPPI	RGGWCCY	2 cut(s)	732, 1513
PstNI	CAGNNNCTG	1 cut(s)	1358
PsuI	RGATCY	3 cut(s)	40, 1420, 2176
PsyI	GACNNNGTC	1 cut(s)	3374
PvuI	CGATCG	1 cut(s)	119
RruI	TCGCGA	1 cut(s)	2121
RsaI	GTAC	7 cut(s)	158, 580, 684, 770, 1270, 1678, 1936
RsaNI	GTAC	7 cut(s)	157, 579, 683, 769, 1269, 1677, 1935
RseI	CAYNNNNRTG	7 cut(s)	1041, 1208, 2332, 2535, 2578, 2640, 3383
Rsr2I	CGGWCCG	1 cut(s)	2286
RsrII	CGGWCCG	1 cut(s)	2286
SacI	GAGCTC	1 cut(s)	466
SalI	GTCGAC	1 cut(s)	1915
SapI	GCTCTTC	2 cut(s)	2398, 2632
SatI	GCNGC	4 cut(s)	88, 633, 1085, 2975
Sau96I	GGNCC	9 cut(s)	27, 732, 762, 967, 1513, 1579, 1663, 2286, 3013
SchI	GAGTC	5 cut(s)	158, 1823, 1847, 2399, 3063
SduI	GDGCHC	4 cut(s)	243, 466, 3070, 3202
SfaNI	GCATC	7 cut(s)	442, 1045, 1082, 1623, 1933, 2478, 2510
SfcI	CTRYAG	2 cut(s)	882, 2199
SfuI	TTCGAA	4 cut(s)	653, 1007, 1589, 3329
SinI	GGWCC	8 cut(s)	732, 762, 967, 1513, 1579, 1663, 2286, 3013
SmiMI	CAYNNNNRTG	7 cut(s)	1041, 1208, 2332, 2535, 2578, 2640, 3383
SmlI	CTYRAG	5 cut(s)	497, 1658, 2349, 2998, 3169
SmoI	CTYRAG	5 cut(s)	497, 1658, 2349, 2998, 3169
SphI	GCATGC	1 cut(s)	3081
SsiI	CCGC	5 cut(s)	53, 87, 403, 2318, 3195
SspMI	CTAG	9 cut(s)	423, 1151, 1155, 1310, 2364, 2400, 2492, 2576, 2909
SstI	GAGCTC	1 cut(s)	466
StyI	CCWWGG	2 cut(s)	1389, 1516
TaaI	ACNGT	6 cut(s)	74, 220, 569, 1139, 2062, 2286
TaiI	ACGT	2 cut(s)	2113, 3378
TatI	WGTACW	3 cut(s)	768, 1268, 1676
TauI	GCSGC	1 cut(s)	90
TseFI	GTSAC	2 cut(s)	80, 557
TseI	GCWGC	3 cut(s)	632, 1084, 2974
Tsp45I	GTSAC	2 cut(s)	80, 557
TspGWI	ACGGA	8 cut(s)	114, 596, 852, 1778, 2278, 2855, 3038, 3126
Tth111I	GACNNNGTC	1 cut(s)	3374
Vha464I	CTTAAG	1 cut(s)	3169
VpaK11BI	GGWCC	8 cut(s)	732, 762, 967, 1513, 1579, 1663, 2286, 3013
XapI	RAATTY	7 cut(s)	245, 1009, 1437, 1784, 1818, 2460, 3006
XbaI	TCTAGA	1 cut(s)	2908
XceI	RCATGY	3 cut(s)	205, 1050, 3081
XcmI	CCANNNNNNNNNTGG	1 cut(s)	184
XmiI	GTMKAC	2 cut(s)	1916, 2281
XspI	CTAG	9 cut(s)	423, 1151, 1155, 1310, 2364, 2400, 2492, 2576, 2909
Zsp2I	ATGCAT	1 cut(s)	1048

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.