MD06G1189300.v1.1

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008866
Physical Location & Seq
Forward (+)
32619760 .. 32620794
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1189300.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGTTGGTCGACCTTTCCCACAACCCTCTCCCTCTTCACTTCCCATGGCTCATGCTTCCTGACCACCACCGTCACCACCACCACCACCACCACCGCGCTCATGACGGCACTGAAGTCTACGAAAACCAACAGCTGAAGTGTTTCTTCAGCCTCTACACCAACCAAACCCACTGTCTCGAACTGCCCGAGGCTTTCGAAAAGACTGTATGTGGCTCGTCATCCGGTTGGCTGATCTTGCGCGACGAAACCACGTCGTCGGTCATCCTCCTCAACCCTCTCAACCGCGCCCAAATCGCTCTCCCAGAGCTCTCCTCCTCTCCGTTTTACGATCAAGACCCTTCACGTGTTCTTCACAAAGCTGTGTTGTCCTCAGACCCTTCTCGCTGTCCATTCGATTACAAAGTGTTGGCGGTTTTCGGAGAGAAGCGAAGGCTCATATGCTACAAAGCGAGGGACAAGACATGGACGATACTACACGATGCAGGGAATAACTATGAAGACATTATTTTGTTTAATGGGGAGGAGTTGCTTGCCGCTACTGACATTGGAAAGCTCGTTTCGTGCGATCTTGATGACGATGGGTGGTTTTCGCTGAGCGTTAATGAAATCGCTCCCCCTTTTACCTTCAAAGGAGGCAAAGTTTATCTTGTCGGCATTGGCGGAGAGCTCGTGATGCTGATAAGGTTTGTGCAGGACAACCCTCGTTACGGCCATGAGACTTATGACTTTGAGGCTTACAGGTATGAACAGTTCGAAAGCGAATGGGAAGCCATCAACGGCTTAGGGGAGTGGACGATTTTCGTGGGGAAAAACCACACTACCGCCATTAAAGCTCAAGATTTCAAGGGCCTGATAAAACCGAACTGCATCTACTTCACTGACGATAGTTTGGACATGGAAGATAGCTTGATGGTTGGCCACGATTCGGGAATCTTTAGCATGGAAGAAGGAAAATTTGAGGCGCTCGGATGTGATTTGAGTGGATTCATGTACGTTTGGCCTCGTCCAGTCTGGTTTATGCCTAACATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

345

Amino Acids

39.43

Weight (kDa)

5.18

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 48 - 312 9.5e-47 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 9, 117
AccII CGCG 3 cut(s) 96, 240, 285
AciI CCGC 6 cut(s) 94, 283, 410, 534, 660, 822
AcoI YGGCCR 2 cut(s) 709, 916
AcsI RAATTY 1 cut(s) 953
AcuI CTGAAG 3 cut(s) 130, 132, 155
AcvI CACGTG 1 cut(s) 344
AfaI GTAC 1 cut(s) 992
AfiI CCNNNNNNNGG 2 cut(s) 707, 925
AflIII ACRYGT 1 cut(s) 343
AgsI TTSAA 2 cut(s) 628, 844
AjiI CACGTC 1 cut(s) 252
AluBI AGCT 7 cut(s) 133, 307, 359, 553, 667, 833, 906
AluI AGCT 7 cut(s) 133, 307, 359, 553, 667, 833, 906
Alw21I GWGCWC 2 cut(s) 309, 669
Alw26I GTCTC 2 cut(s) 179, 710
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 4 cut(s) 709, 847, 916, 998
ApoI RAATTY 1 cut(s) 953
ArsI GACNNNNNNTTYG 2 cut(s) 157, 189
Asp700I GAANNNNTTC 1 cut(s) 140
AspLEI GCGC 4 cut(s) 98, 240, 287, 964
AspS9I GGNCC 1 cut(s) 847
AsuHPI GGTGA 1 cut(s) 65
AsuII TTCGAA 2 cut(s) 195, 753
AvaI CYCGRG 1 cut(s) 185
BalI TGGCCA 1 cut(s) 918
BanII GRGCYC 2 cut(s) 309, 669
BauI CACGAG 1 cut(s) 668
BbrPI CACGTG 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 504
Bbv12I GWGCWC 2 cut(s) 309, 669
BccI CCATC 3 cut(s) 572, 779, 904
BceAI ACGGC 3 cut(s) 121, 724, 793
BcoDI GTCTC 2 cut(s) 179, 710
BfoI RGCGCY 1 cut(s) 965
BisI GCNGC 1 cut(s) 534
BlpI GCTNAGC 1 cut(s) 593
BlsI GCNGC 1 cut(s) 535
BmeT110I CYCGRG 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 847
BmsI GCATC 3 cut(s) 469, 663, 876
BpiI GAAGAC 1 cut(s) 504
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu10I CCTNAGC 1 cut(s) 781
Bpu1102I GCTNAGC 1 cut(s) 593
Bpu14I TTCGAA 2 cut(s) 195, 753
BpuEI CTTGAG 1 cut(s) 819
BsaAI YACGTR 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 44, 186
BsaWI WCCGGW 1 cut(s) 221
BsaXI ACNNNNNCTCC 4 cut(s) 624, 654, 654, 684
Bsc4I CCNNNNNNNGG 2 cut(s) 707, 925
Bse1I ACTGG 1 cut(s) 1007
BseDI CCNNGG 2 cut(s) 44, 186
BseGI GGATG 3 cut(s) 218, 261, 974
BseLI CCNNNNNNNGG 2 cut(s) 707, 925
BseMII CTCAG 2 cut(s) 384, 584
BseNI ACTGG 1 cut(s) 1007
BseRI GAGGAG 4 cut(s) 257, 301, 304, 536
BsgI GTGCAG 1 cut(s) 710
Bsh1236I CGCG 3 cut(s) 96, 240, 285
BshFI GGCC 4 cut(s) 711, 849, 918, 1000
BsiHKAI GWGCWC 2 cut(s) 309, 669
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 2 cut(s) 707, 925
BsmAI GTCTC 2 cut(s) 179, 710
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 4 cut(s) 711, 849, 918, 1000
BsoBI CYCGRG 1 cut(s) 185
Bsp119I TTCGAA 2 cut(s) 195, 753
Bsp1286I GDGCHC 2 cut(s) 309, 669
Bsp143I GATC 3 cut(s) 231, 328, 565
Bsp1720I GCTNAGC 1 cut(s) 593
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 6 cut(s) 94, 283, 410, 534, 660, 822
BspANI GGCC 4 cut(s) 711, 849, 918, 1000
BspCNI CTCAG 2 cut(s) 383, 585
BspFNI CGCG 3 cut(s) 96, 240, 285
BspHI TCATGA 1 cut(s) 100
BspT104I TTCGAA 2 cut(s) 195, 753
BsrI ACTGG 1 cut(s) 1007
BssECI CCNNGG 2 cut(s) 44, 186
BssMI GATC 3 cut(s) 231, 328, 565
BssSI CACGAG 1 cut(s) 668
BssT1I CCWWGG 1 cut(s) 44
Bst2BI CACGAG 1 cut(s) 668
Bst4CI ACNGT 4 cut(s) 71, 173, 205, 750
Bst6I CTCTTC 1 cut(s) 39
BstBAI YACGTR 1 cut(s) 344
BstBI TTCGAA 2 cut(s) 195, 753
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 3 cut(s) 370, 593, 781
BstDSI CCRYGG 1 cut(s) 44
BstF5I GGATG 3 cut(s) 218, 261, 974
BstFNI CGCG 3 cut(s) 96, 240, 285
BstH2I RGCGCY 1 cut(s) 965
BstHHI GCGC 4 cut(s) 98, 240, 287, 964
BstKTI GATC 3 cut(s) 234, 331, 568
BstMAI GTCTC 2 cut(s) 179, 710
BstMBI GATC 3 cut(s) 231, 328, 565
BstMWI GCNNNNNNNGC 4 cut(s) 235, 293, 673, 830
BstUI CGCG 3 cut(s) 96, 240, 285
BstV2I GAAGAC 1 cut(s) 504
BsuRI GGCC 4 cut(s) 711, 849, 918, 1000
BtgI CCRYGG 1 cut(s) 44
BtrI CACGTC 1 cut(s) 252
BtsCI GGATG 3 cut(s) 218, 261, 974
BtsIMutI CAGTG 3 cut(s) 108, 169, 876
Cac8I GCNNGC 1 cut(s) 531
CciI TCATGA 1 cut(s) 100
CfoI GCGC 4 cut(s) 98, 240, 287, 964
Cfr13I GGNCC 1 cut(s) 847
Csp6I GTAC 1 cut(s) 991
CviAII CATG 9 cut(s) 45, 52, 101, 462, 713, 895, 940, 988, 1027
CviQI GTAC 1 cut(s) 991
DdeI CTNAG 3 cut(s) 370, 593, 781
DpnI GATC 3 cut(s) 233, 330, 567
DpnII GATC 3 cut(s) 231, 328, 565
EaeI YGGCCR 2 cut(s) 709, 916
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 1 cut(s) 675
Ecl136II GAGCTC 2 cut(s) 307, 667
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 2 cut(s) 309, 669
Eco53kI GAGCTC 2 cut(s) 307, 667
Eco57I CTGAAG 3 cut(s) 130, 132, 155
Eco72I CACGTG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 185
EcoICRI GAGCTC 2 cut(s) 307, 667
EcoO109I RGGNCCY 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 44
EcoT38I GRGCYC 2 cut(s) 309, 669
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 9 cut(s) 48, 55, 104, 465, 716, 898, 943, 991, 1030
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 630, 662
FaqI GGGAC 1 cut(s) 467
FatI CATG 9 cut(s) 44, 51, 100, 461, 712, 894, 939, 987, 1026
FauNDI CATATG 1 cut(s) 437
FblI GTMKAC 2 cut(s) 9, 117
Fnu4HI GCNGC 1 cut(s) 534
FokI GGATG 3 cut(s) 205, 248, 981
FriOI GRGCYC 2 cut(s) 309, 669
Fsp4HI GCNGC 1 cut(s) 534
GlaI GCGC 4 cut(s) 97, 239, 286, 963
GluI GCNGC 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 965
HaeIII GGCC 4 cut(s) 711, 849, 918, 1000
HapII CCGG 1 cut(s) 222
HhaI GCGC 4 cut(s) 98, 240, 287, 964
Hin1II CATG 9 cut(s) 48, 55, 104, 465, 716, 898, 943, 991, 1030
Hin6I GCGC 4 cut(s) 96, 238, 285, 962
HinP1I GCGC 4 cut(s) 96, 238, 285, 962
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 3 cut(s) 923, 930, 984
HpaII CCGG 1 cut(s) 222
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 3 cut(s) 10, 118, 792
Hpy188I TCNGA 3 cut(s) 373, 419, 968
Hpy188III TCNNGA 8 cut(s) 59, 101, 176, 332, 569, 670, 836, 927
Hpy8I GTNNAC 3 cut(s) 10, 118, 792
Hpy99I CGWCG 3 cut(s) 245, 256, 259
HpyAV CCTTC 5 cut(s) 348, 387, 423, 634, 941
HpyCH4III ACNGT 4 cut(s) 71, 173, 205, 750
HpyCH4IV ACGT 3 cut(s) 251, 343, 993
HpyCH4V TGCA 3 cut(s) 482, 691, 867
HpyF10VI GCNNNNNNNGC 4 cut(s) 235, 293, 673, 830
HpyF3I CTNAG 3 cut(s) 370, 593, 781
HpySE526I ACGT 3 cut(s) 251, 343, 993
Hsp92II CATG 9 cut(s) 48, 55, 104, 465, 716, 898, 943, 991, 1030
HspAI GCGC 4 cut(s) 96, 238, 285, 962
Kzo9I GATC 3 cut(s) 231, 328, 565
LmnI GCTCC 1 cut(s) 616
LpnPI CCDG 9 cut(s) 72, 235, 315, 468, 677, 724, 863, 997, 1020
LweI GCATC 3 cut(s) 469, 663, 876
MaeII ACGT 3 cut(s) 251, 343, 993
MaeIII GTNAC 2 cut(s) 71, 704
MalI GATC 3 cut(s) 233, 330, 567
MboI GATC 3 cut(s) 231, 328, 565
MboII GAAGA 6 cut(s) 26, 136, 341, 509, 911, 956
MhlI GDGCHC 2 cut(s) 309, 669
MlsI TGGCCA 1 cut(s) 918
MluCI AATT 1 cut(s) 953
MluNI TGGCCA 1 cut(s) 918
Mox20I TGGCCA 1 cut(s) 918
MroXI GAANNNNTTC 1 cut(s) 140
MscI TGGCCA 1 cut(s) 918
MseI TTAA 3 cut(s) 513, 600, 828
Msp20I TGGCCA 1 cut(s) 918
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 1 cut(s) 222
MvnI CGCG 3 cut(s) 96, 240, 285
MwoI GCNNNNNNNGC 4 cut(s) 235, 293, 673, 830
NcoI CCATGG 1 cut(s) 44
NdeI CATATG 1 cut(s) 437
NdeII GATC 3 cut(s) 231, 328, 565
NlaIII CATG 9 cut(s) 48, 55, 104, 465, 716, 898, 943, 991, 1030
NmuCI GTSAC 1 cut(s) 71
NspV TTCGAA 2 cut(s) 195, 753
PagI TCATGA 1 cut(s) 100
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 561
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 3 cut(s) 923, 930, 984
PkrI GCNGC 1 cut(s) 535
PmaCI CACGTG 1 cut(s) 344
PmlI CACGTG 1 cut(s) 344
Ppu21I YACGTR 1 cut(s) 344
Psp124BI GAGCTC 2 cut(s) 309, 669
PspCI CACGTG 1 cut(s) 344
PspPI GGNCC 1 cut(s) 847
PsrI GAACNNNNNNTAC 4 cut(s) 734, 766, 854, 886
PvuII CAGCTG 1 cut(s) 133
RsaI GTAC 1 cut(s) 992
RsaNI GTAC 1 cut(s) 991
SacI GAGCTC 2 cut(s) 309, 669
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 513, 600, 828
SatI GCNGC 1 cut(s) 534
Sau3AI GATC 3 cut(s) 231, 328, 565
Sau96I GGNCC 1 cut(s) 847
SduI GDGCHC 2 cut(s) 309, 669
SfaNI GCATC 3 cut(s) 469, 663, 876
SfuI TTCGAA 2 cut(s) 195, 753
SmlI CTYRAG 1 cut(s) 834
SmoI CTYRAG 1 cut(s) 834
Sse9I AATT 1 cut(s) 953
SsiI CCGC 6 cut(s) 94, 283, 410, 534, 660, 822
SstI GAGCTC 2 cut(s) 309, 669
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 4 cut(s) 71, 173, 205, 750
TaiI ACGT 3 cut(s) 254, 346, 996
TaqI TCGA 5 cut(s) 9, 177, 195, 393, 753
TaqII GACCGA 1 cut(s) 247
TasI AATT 1 cut(s) 953
TauI GCSGC 1 cut(s) 536
TfiI GAWTC 3 cut(s) 923, 930, 984
Tru1I TTAA 3 cut(s) 513, 600, 828
Tru9I TTAA 3 cut(s) 513, 600, 828
TscAI CASTG 3 cut(s) 115, 176, 883
TseFI GTSAC 1 cut(s) 71
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 4 cut(s) 510, 618, 759, 976
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 3 cut(s) 115, 176, 883
XapI RAATTY 1 cut(s) 953
XmiI GTMKAC 2 cut(s) 9, 117
XmnI GAANNNNTTC 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.