RLG00000005233

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008866
Physical Location & Seq
Reverse (-)
66135826 .. 66136773
948 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005233

Sequence Viewer

Length: 948 bp
ATGGTGAACTTTTACACCAAACCCCTTCATGAAAACTCCCCATGGCTAATGCTTCCGAGCCAGTACCAACCTGAAGAACATCACATTATCACCAAACACTCCAGATTCTTCCTCAACGTCTCCACCAACCAAGTCCACGGTTTCGAGCTACCCAAAAACGATCATGAAAAGACCGTATGTGGATTCTCGGCCGATTGGCTCATCTTGTTGGATGGCCAAAACTCATCACTCACTCTCCTCAACCCATTCACCGGCCACCAAGTCAACAATCTCCCACAGCTCCCTCCAAACGGCGCGGTCCACAAGGCCATATTGTCCACAGACCCGTTATGCAACCCTAGAGACTACAAAGTCCTGGCAATCTTCGGAGAGAAGCGAAACCTACTTTGTTACGAAGACGGTAACCAGGAGTGGACTGTTTTACAAGGTTATGGGAACCAGTACGACGACGTTCTTGCATCCACAGACAAGGAGTTGCTTGCAGTGAATGAACTAGGGAGGCTTGTACTTTGTGATCTTGATTGTAGGTTTTCACTGGAATATAATGAGCGTGCATTACCTTCATTCTTTAACGGGAACAAGGTTTATTTAGTGAGCCTAGAAGGACTGGAATTTATACTGTTGAGCTTTTCGGGGACAAGCTATGTTTTTGGGCATCAAAGCTATGACGATAACCTTAGAAGGTTTGAGGTTTACTATTATGTTGAAAAGAAGTGGCACTACACCACAGATTTGGGTGAGAGGACGATTTTCTTGGGACATAACCACTCGGTAGCTGTTTGTGATGCGCCGGGTTATAGACGTAATTGCATCTACTTCACCGGTGATGAGGAAGGTGAAATTGATAACTCGGGAGTGTTTAGTTTGAAAGATGGGGAAGCTGAAAGACTGCAATGGTTTATACCTGGTACTACTGCTGCAAGGAAACTTGTAATATACAATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

316

Amino Acids

36.15

Weight (kDa)

5.35

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 37 - 288 8.7e-40 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AccII CGCG 1 cut(s) 296
AciI CCGC 1 cut(s) 296
AcoI YGGCCR 3 cut(s) 189, 214, 253
AcsI RAATTY 1 cut(s) 611
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 4 cut(s) 65, 443, 507, 910
AfiI CCNNNNNNNGG 2 cut(s) 251, 290
AgeI ACCGGT 1 cut(s) 821
AgsI TTSAA 2 cut(s) 707, 868
AjnI CCWGG 3 cut(s) 354, 405, 904
AluBI AGCT 7 cut(s) 148, 280, 627, 642, 663, 776, 881
AluI AGCT 7 cut(s) 148, 280, 627, 642, 663, 776, 881
Alw26I GTCTC 2 cut(s) 124, 336
Ama87I CYCGRG 1 cut(s) 850
AoxI GGCC 4 cut(s) 189, 214, 253, 306
ApeKI GCWGC 1 cut(s) 917
ApoI RAATTY 1 cut(s) 611
AsiGI ACCGGT 1 cut(s) 821
AspLEI GCGC 2 cut(s) 296, 790
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 7 cut(s) 16, 82, 241, 749, 811, 836, 848
AvaI CYCGRG 1 cut(s) 850
AvaII GGWCC 1 cut(s) 298
BalI TGGCCA 1 cut(s) 216
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbsI GAAGAC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 904
BccI CCATC 2 cut(s) 206, 866
BceAI ACGGC 1 cut(s) 307
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 3 cut(s) 356, 407, 906
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 2 cut(s) 124, 336
BfaI CTAG 3 cut(s) 339, 494, 599
BisI GCNGC 1 cut(s) 918
BlsI GCNGC 1 cut(s) 919
Bme1390I CCNGG 4 cut(s) 356, 407, 792, 906
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 850
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 437
BmrFI CCNGG 4 cut(s) 356, 407, 792, 906
BmsI GCATC 4 cut(s) 467, 664, 775, 819
BpiI GAAGAC 1 cut(s) 402
BpmI CTGGAG 1 cut(s) 85
BpuMI CCSGG 1 cut(s) 792
BsaJI CCNNGG 2 cut(s) 41, 136
BsaWI WCCGGW 1 cut(s) 821
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 290
Bse118I RCCGGY 2 cut(s) 251, 821
Bse1I ACTGG 4 cut(s) 61, 439, 540, 612
Bse3DI GCAATG 1 cut(s) 899
BseBI CCWGG 3 cut(s) 356, 407, 906
BseDI CCNNGG 2 cut(s) 41, 136
BseGI GGATG 2 cut(s) 217, 458
BseLI CCNNNNNNNGG 2 cut(s) 251, 290
BseMI GCAATG 1 cut(s) 899
BseNI ACTGG 4 cut(s) 61, 439, 540, 612
BseRI GAGGAG 1 cut(s) 227
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 1 cut(s) 904
Bsh1236I CGCG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 4 cut(s) 191, 216, 255, 308
BshTI ACCGGT 1 cut(s) 821
BsiEI CGRYCG 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 850
BsiSI CCGG 3 cut(s) 252, 791, 822
BslFI GGGAC 2 cut(s) 649, 771
BslI CCNNNNNNNGG 2 cut(s) 251, 290
BsmAI GTCTC 2 cut(s) 124, 336
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 2 cut(s) 649, 771
BsnI GGCC 4 cut(s) 191, 216, 255, 308
BsoBI CYCGRG 1 cut(s) 850
Bsp143I GATC 2 cut(s) 160, 514
Bsp19I CCATGG 1 cut(s) 41
BspACI CCGC 1 cut(s) 296
BspANI GGCC 4 cut(s) 191, 216, 255, 308
BspFNI CGCG 1 cut(s) 296
BspHI TCATGA 2 cut(s) 28, 163
BspLI GGNNCC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 899
BsrFI RCCGGY 2 cut(s) 251, 821
BsrI ACTGG 4 cut(s) 61, 439, 540, 612
BssAI RCCGGY 2 cut(s) 251, 821
BssECI CCNNGG 2 cut(s) 41, 136
BssMI GATC 2 cut(s) 160, 514
BssT1I CCWWGG 1 cut(s) 41
Bst2UI CCWGG 3 cut(s) 356, 407, 906
Bst4CI ACNGT 5 cut(s) 140, 175, 401, 418, 621
BstC8I GCNNGC 2 cut(s) 480, 552
BstDEI CTNAG 1 cut(s) 677
BstDSI CCRYGG 2 cut(s) 41, 136
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 2 cut(s) 217, 458
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 2 cut(s) 296, 790
BstKTI GATC 2 cut(s) 163, 517
BstMAI GTCTC 2 cut(s) 124, 336
BstMBI GATC 2 cut(s) 160, 514
BstMCI CGRYCG 1 cut(s) 192
BstNI CCWGG 3 cut(s) 356, 407, 906
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 4 cut(s) 354, 405, 790, 904
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 1 cut(s) 904
BstV2I GAAGAC 1 cut(s) 402
BstXI CCANNNNNNTGG 1 cut(s) 733
BstZI CGGCCG 1 cut(s) 189
BsuRI GGCC 4 cut(s) 191, 216, 255, 308
BtgI CCRYGG 2 cut(s) 41, 136
BtsCI GGATG 2 cut(s) 217, 458
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 2 cut(s) 489, 533
Cac8I GCNNGC 2 cut(s) 480, 552
CciI TCATGA 2 cut(s) 28, 163
CfoI GCGC 2 cut(s) 296, 790
Cfr10I RCCGGY 2 cut(s) 251, 821
Cfr13I GGNCC 1 cut(s) 298
CsiI ACCWGGT 1 cut(s) 904
Csp6I GTAC 4 cut(s) 64, 442, 506, 909
CspAI ACCGGT 1 cut(s) 821
CviAII CATG 3 cut(s) 29, 42, 164
CviQI GTAC 4 cut(s) 64, 442, 506, 909
DdeI CTNAG 1 cut(s) 677
DpnI GATC 2 cut(s) 162, 516
DpnII GATC 2 cut(s) 160, 514
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 3 cut(s) 189, 214, 253
EagI CGGCCG 1 cut(s) 189
EclXI CGGCCG 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 298
Eco52I CGGCCG 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 850
Eco91I GGTNACC 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 354, 405, 904
EcoT14I CCWWGG 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 3 cut(s) 32, 45, 167
FaqI GGGAC 2 cut(s) 649, 771
FatI CATG 3 cut(s) 28, 41, 163
Fnu4HI GCNGC 1 cut(s) 918
FokI GGATG 2 cut(s) 224, 445
Fsp4HI GCNGC 1 cut(s) 918
FspBI CTAG 3 cut(s) 339, 494, 599
GlaI GCGC 2 cut(s) 295, 789
GluI GCNGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 85
HaeIII GGCC 4 cut(s) 191, 216, 255, 308
HapII CCGG 3 cut(s) 252, 791, 822
HhaI GCGC 2 cut(s) 296, 790
Hin1II CATG 3 cut(s) 32, 45, 167
Hin6I GCGC 2 cut(s) 294, 788
HinP1I GCGC 2 cut(s) 294, 788
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 2 cut(s) 105, 183
HpaII CCGG 3 cut(s) 252, 791, 822
HphI GGTGA 7 cut(s) 16, 82, 241, 749, 811, 836, 848
Hpy166II GTNNAC 7 cut(s) 7, 136, 265, 301, 318, 414, 694
Hpy188I TCNGA 2 cut(s) 57, 368
Hpy188III TCNNGA 5 cut(s) 29, 102, 164, 518, 852
Hpy8I GTNNAC 7 cut(s) 7, 136, 265, 301, 318, 414, 694
Hpy99I CGWCG 2 cut(s) 449, 452
HpyAV CCTTC 5 cut(s) 35, 570, 596, 675, 827
HpyCH4III ACNGT 5 cut(s) 140, 175, 401, 418, 621
HpyCH4IV ACGT 3 cut(s) 117, 450, 802
HpyCH4V TGCA 7 cut(s) 333, 458, 482, 554, 810, 892, 920
HpyF3I CTNAG 1 cut(s) 677
HpySE526I ACGT 3 cut(s) 117, 450, 802
Hsp92II CATG 3 cut(s) 32, 45, 167
HspAI GCGC 2 cut(s) 294, 788
Kzo9I GATC 2 cut(s) 160, 514
LmnI GCTCC 1 cut(s) 285
Lsp1109I GCAGC 1 cut(s) 904
LweI GCATC 4 cut(s) 467, 664, 775, 819
MabI ACCWGGT 1 cut(s) 904
MaeI CTAG 3 cut(s) 339, 494, 599
MaeII ACGT 3 cut(s) 117, 450, 802
MaeIII GTNAC 2 cut(s) 389, 401
MalI GATC 2 cut(s) 162, 516
MboI GATC 2 cut(s) 160, 514
MboII GAAGA 4 cut(s) 86, 100, 355, 407
MlsI TGGCCA 1 cut(s) 216
MluCI AATT 3 cut(s) 611, 805, 840
MluNI TGGCCA 1 cut(s) 216
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 7 cut(s) 122, 248, 294, 492, 682, 735, 823
Mox20I TGGCCA 1 cut(s) 216
MscI TGGCCA 1 cut(s) 216
MseI TTAA 1 cut(s) 570
Msp20I TGGCCA 1 cut(s) 216
MspI CCGG 3 cut(s) 252, 791, 822
MspR9I CCNGG 4 cut(s) 356, 407, 792, 906
MvaI CCWGG 3 cut(s) 356, 407, 906
MvnI CGCG 1 cut(s) 296
NciI CCSGG 1 cut(s) 792
NcoI CCATGG 1 cut(s) 41
NdeII GATC 2 cut(s) 160, 514
NlaIII CATG 3 cut(s) 32, 45, 167
NlaIV GGNNCC 1 cut(s) 437
NmeAIII GCCGAG 1 cut(s) 167
PagI TCATGA 2 cut(s) 28, 163
PfeI GAWTC 2 cut(s) 105, 183
PinAI ACCGGT 1 cut(s) 821
PkrI GCNGC 1 cut(s) 919
Psp6I CCWGG 3 cut(s) 354, 405, 904
PspEI GGTNACC 1 cut(s) 401
PspGI CCWGG 3 cut(s) 354, 405, 904
PspN4I GGNNCC 1 cut(s) 437
PspPI GGNCC 1 cut(s) 298
RsaI GTAC 4 cut(s) 65, 443, 507, 910
RsaNI GTAC 4 cut(s) 64, 442, 506, 909
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 1 cut(s) 918
Sau3AI GATC 2 cut(s) 160, 514
Sau96I GGNCC 1 cut(s) 298
ScrFI CCNGG 4 cut(s) 356, 407, 792, 906
SexAI ACCWGGT 1 cut(s) 904
SfaNI GCATC 4 cut(s) 467, 664, 775, 819
SgrAI CRCCGGYG 1 cut(s) 821
SinI GGWCC 1 cut(s) 298
Sse9I AATT 3 cut(s) 611, 805, 840
SsiI CCGC 1 cut(s) 296
SspMI CTAG 3 cut(s) 339, 494, 599
StyD4I CCNGG 4 cut(s) 354, 405, 790, 904
StyI CCWWGG 1 cut(s) 41
TaaI ACNGT 5 cut(s) 140, 175, 401, 418, 621
TaiI ACGT 3 cut(s) 120, 453, 805
TaqI TCGA 1 cut(s) 144
TasI AATT 3 cut(s) 611, 805, 840
TatI WGTACW 1 cut(s) 505
TfiI GAWTC 2 cut(s) 105, 183
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 2 cut(s) 489, 540
TseI GCWGC 1 cut(s) 917
TspDTI ATGAA 5 cut(s) 17, 45, 180, 504, 552
TspRI CASTG 2 cut(s) 489, 540
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 1 cut(s) 611
XspI CTAG 3 cut(s) 339, 494, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.