Rroxscaffold_3G00272570

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008866
Physical Location & Seq
Reverse (-)
64643132 .. 64644031
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272570.1

Sequence Viewer

Length: 900 bp
ATGCTTCCGAGACAGTACCAACCTGAAGAACATCACATTATCACCAAACACTCCAGATTCTTCCTCAACGTTTCCACCAACCAAGTCCACCGTTTCGAGCTACCCAAAAACGATCATGAAAAGACCGTATGTGGATTCTCGGCCGATTGGCTCATCTTGTTGGATGGCCAAAACTCATCACTCAGTCTCCTCAACCCATTCACCGGCCACCAAGTCAACAATCTCCCACGGCTCCCTCCAAAGGGCGCCGTCCACAAGGCCATATTGTCCTCAGACCCGTTATGCAACCCTAGAGACTACAAAGTCCTGGCAATCTTCGGAGAGAAGCGAAACCTAATTTGTTACGAAGACGGTAACCAGGAGTGGACTGTTTTACAAGGTTATGGGAACCAGTACGACGACGTTCTTGCATCCACAGACAAGGAGTTGCTTGCAGTGAATGAACTAGGGAGGCTTGTACTTTGTGATCTTGATTGTAGGTTTTCACTGGAATATAATGAGCGTGCATTACCTTCATTCTTTAACGGGAACAAGGTTTATTTAGTGAGCCCAGAAGGACTGGAATTTATACTGTTGAGCTTTTCGGGGACAAGCTATGTTTTTGGGCGTCAAAGCTATGACGATAACCTTAGAAGGTTTGAGGTTTACTATTATGTTGAAAAGAAGTGGCACTACATCACAGATTTGGGTGAGAGGACGATTTTCTTGGGACATAACCACTCGGTAGCTGTTTGTGATGCTCCGGGTTATAGACGTAATTGCATCTACTTCACCGGTGACGAGGAAGGTGAAATTGATAACTCGGGAGTGTTTAGTTTGAAAGATGGGGAAGCTGAAAGGCTGCAATGGTTTATGCCTGGTACTACTGCTGCAAGGAAACTTGTAACATACAATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

299

Amino Acids

34.4

Weight (kDa)

5.7

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 21 - 272 3.2e-40 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 245
AclI AACGTT 1 cut(s) 69
AcoI YGGCCR 3 cut(s) 141, 166, 205
AcsI RAATTY 1 cut(s) 563
AcuI CTGAAG 1 cut(s) 45
AcyI GRCGYC 2 cut(s) 246, 607
AfaI GTAC 4 cut(s) 17, 395, 459, 862
AfiI CCNNNNNNNGG 3 cut(s) 203, 241, 242
AgeI ACCGGT 1 cut(s) 773
AgsI TTSAA 2 cut(s) 659, 820
AjnI CCWGG 3 cut(s) 306, 357, 856
AluBI AGCT 6 cut(s) 100, 579, 594, 615, 728, 833
AluI AGCT 6 cut(s) 100, 579, 594, 615, 728, 833
Alw26I GTCTC 3 cut(s) 4, 191, 288
Ama87I CYCGRG 1 cut(s) 802
AoxI GGCC 4 cut(s) 141, 166, 205, 258
ApeKI GCWGC 2 cut(s) 841, 869
ApoI RAATTY 1 cut(s) 563
AsiGI ACCGGT 1 cut(s) 773
AspLEI GCGC 1 cut(s) 248
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 6 cut(s) 34, 193, 701, 763, 788, 800
AvaI CYCGRG 1 cut(s) 802
BalI TGGCCA 1 cut(s) 168
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 551
BarI GAAGNNNNNNTAC 2 cut(s) 656, 688
BbsI GAAGAC 1 cut(s) 354
BbvI GCAGC 2 cut(s) 828, 856
BccI CCATC 2 cut(s) 158, 818
BceAI ACGGC 2 cut(s) 233, 245
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 3 cut(s) 308, 359, 858
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 3 cut(s) 4, 191, 288
BfaI CTAG 2 cut(s) 291, 446
BfoI RGCGCY 1 cut(s) 249
BisI GCNGC 2 cut(s) 842, 870
BlsI GCNGC 2 cut(s) 843, 871
Bme1390I CCNGG 4 cut(s) 308, 359, 744, 858
BmeT110I CYCGRG 1 cut(s) 802
BmiI GGNNCC 3 cut(s) 233, 247, 389
BmrFI CCNGG 4 cut(s) 308, 359, 744, 858
BmsI GCATC 3 cut(s) 419, 727, 771
BpiI GAAGAC 1 cut(s) 354
BpmI CTGGAG 1 cut(s) 37
BpuMI CCSGG 1 cut(s) 744
BsaHI GRCGYC 2 cut(s) 246, 607
BsaJI CCNNGG 1 cut(s) 227
BsaWI WCCGGW 1 cut(s) 773
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 241, 242
Bse118I RCCGGY 2 cut(s) 203, 773
Bse1I ACTGG 3 cut(s) 391, 492, 564
Bse3DI GCAATG 1 cut(s) 851
BseBI CCWGG 3 cut(s) 308, 359, 858
BseDI CCNNGG 1 cut(s) 227
BseGI GGATG 2 cut(s) 169, 410
BseLI CCNNNNNNNGG 3 cut(s) 203, 241, 242
BseMI GCAATG 1 cut(s) 851
BseMII CTCAG 2 cut(s) 196, 285
BseNI ACTGG 3 cut(s) 391, 492, 564
BseRI GAGGAG 1 cut(s) 179
BseX3I CGGCCG 1 cut(s) 141
BseXI GCAGC 2 cut(s) 828, 856
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 4 cut(s) 143, 168, 207, 260
BshNI GGYRCC 1 cut(s) 245
BshTI ACCGGT 1 cut(s) 773
BsiEI CGRYCG 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 802
BsiSI CCGG 3 cut(s) 204, 743, 774
BslFI GGGAC 2 cut(s) 601, 723
BslI CCNNNNNNNGG 3 cut(s) 203, 241, 242
BsmAI GTCTC 3 cut(s) 4, 191, 288
BsmFI GGGAC 2 cut(s) 601, 723
BsnI GGCC 4 cut(s) 143, 168, 207, 260
BsoBI CYCGRG 1 cut(s) 802
Bsp1286I GDGCHC 1 cut(s) 551
Bsp143I GATC 2 cut(s) 112, 466
BspANI GGCC 4 cut(s) 143, 168, 207, 260
BspCNI CTCAG 2 cut(s) 195, 284
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 3 cut(s) 233, 247, 389
BspT107I GGYRCC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 851
BsrFI RCCGGY 2 cut(s) 203, 773
BsrI ACTGG 3 cut(s) 391, 492, 564
BssAI RCCGGY 2 cut(s) 203, 773
BssECI CCNNGG 1 cut(s) 227
BssMI GATC 2 cut(s) 112, 466
BssNI GRCGYC 2 cut(s) 246, 607
Bst2UI CCWGG 3 cut(s) 308, 359, 858
Bst4CI ACNGT 6 cut(s) 15, 92, 127, 353, 370, 573
BstACI GRCGYC 2 cut(s) 246, 607
BstC8I GCNNGC 2 cut(s) 432, 504
BstDEI CTNAG 3 cut(s) 182, 271, 629
BstDSI CCRYGG 1 cut(s) 227
BstEII GGTNACC 1 cut(s) 353
BstF5I GGATG 2 cut(s) 169, 410
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 2 cut(s) 115, 469
BstMAI GTCTC 3 cut(s) 4, 191, 288
BstMBI GATC 2 cut(s) 112, 466
BstMCI CGRYCG 1 cut(s) 144
BstNI CCWGG 3 cut(s) 308, 359, 858
BstPI GGTNACC 1 cut(s) 353
BstSCI CCNGG 4 cut(s) 306, 357, 742, 856
BstV1I GCAGC 2 cut(s) 828, 856
BstV2I GAAGAC 1 cut(s) 354
BstZI CGGCCG 1 cut(s) 141
BsuRI GGCC 4 cut(s) 143, 168, 207, 260
BtgI CCRYGG 1 cut(s) 227
BtsCI GGATG 2 cut(s) 169, 410
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 2 cut(s) 441, 485
Cac8I GCNNGC 2 cut(s) 432, 504
CciI TCATGA 1 cut(s) 115
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 2 cut(s) 203, 773
CseI GACGC 1 cut(s) 596
Csp6I GTAC 4 cut(s) 16, 394, 458, 861
CspAI ACCGGT 1 cut(s) 773
CviAII CATG 1 cut(s) 116
CviQI GTAC 4 cut(s) 16, 394, 458, 861
DdeI CTNAG 3 cut(s) 182, 271, 629
DinI GGCGCC 1 cut(s) 247
DpnI GATC 2 cut(s) 114, 468
DpnII GATC 2 cut(s) 112, 466
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 3 cut(s) 141, 166, 205
EagI CGGCCG 1 cut(s) 141
EclXI CGGCCG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 551
Eco52I CGGCCG 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 802
Eco91I GGTNACC 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 353
EcoRII CCWGG 3 cut(s) 306, 357, 856
EcoT38I GRGCYC 1 cut(s) 551
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
FaeI CATG 1 cut(s) 119
FaqI GGGAC 2 cut(s) 601, 723
FatI CATG 1 cut(s) 115
Fnu4HI GCNGC 2 cut(s) 842, 870
FokI GGATG 2 cut(s) 176, 397
FriOI GRGCYC 1 cut(s) 551
Fsp4HI GCNGC 2 cut(s) 842, 870
FspBI CTAG 2 cut(s) 291, 446
GlaI GCGC 1 cut(s) 247
GluI GCNGC 2 cut(s) 842, 870
GsuI CTGGAG 1 cut(s) 37
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 4 cut(s) 143, 168, 207, 260
HapII CCGG 3 cut(s) 204, 743, 774
HgaI GACGC 1 cut(s) 596
HhaI GCGC 1 cut(s) 248
Hin1I GRCGYC 2 cut(s) 246, 607
Hin1II CATG 1 cut(s) 119
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 2 cut(s) 57, 135
HpaII CCGG 3 cut(s) 204, 743, 774
HphI GGTGA 6 cut(s) 34, 193, 701, 763, 788, 800
Hpy166II GTNNAC 5 cut(s) 88, 217, 253, 366, 646
Hpy188I TCNGA 3 cut(s) 9, 274, 320
Hpy188III TCNNGA 4 cut(s) 54, 116, 470, 804
Hpy8I GTNNAC 5 cut(s) 88, 217, 253, 366, 646
Hpy99I CGWCG 2 cut(s) 401, 404
HpyAV CCTTC 4 cut(s) 522, 548, 627, 779
HpyCH4III ACNGT 6 cut(s) 15, 92, 127, 353, 370, 573
HpyCH4IV ACGT 3 cut(s) 69, 402, 754
HpyCH4V TGCA 7 cut(s) 285, 410, 434, 506, 762, 844, 872
HpyF3I CTNAG 3 cut(s) 182, 271, 629
HpySE526I ACGT 3 cut(s) 69, 402, 754
Hsp92I GRCGYC 2 cut(s) 246, 607
Hsp92II CATG 1 cut(s) 119
HspAI GCGC 1 cut(s) 246
KasI GGCGCC 1 cut(s) 245
Kzo9I GATC 2 cut(s) 112, 466
LmnI GCTCC 2 cut(s) 237, 745
Lsp1109I GCAGC 2 cut(s) 828, 856
LweI GCATC 3 cut(s) 419, 727, 771
MaeI CTAG 2 cut(s) 291, 446
MaeII ACGT 3 cut(s) 69, 402, 754
MaeIII GTNAC 4 cut(s) 341, 353, 776, 883
MalI GATC 2 cut(s) 114, 468
MboI GATC 2 cut(s) 112, 466
MboII GAAGA 4 cut(s) 38, 52, 307, 359
MhlI GDGCHC 1 cut(s) 551
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 4 cut(s) 336, 563, 757, 792
MluNI TGGCCA 1 cut(s) 168
Mly113I GGCGCC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 8 cut(s) 74, 200, 246, 280, 444, 634, 687, 775
Mox20I TGGCCA 1 cut(s) 168
MscI TGGCCA 1 cut(s) 168
MseI TTAA 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 168
MspI CCGG 3 cut(s) 204, 743, 774
MspR9I CCNGG 4 cut(s) 308, 359, 744, 858
MvaI CCWGG 3 cut(s) 308, 359, 858
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 1 cut(s) 744
NdeII GATC 2 cut(s) 112, 466
NlaIII CATG 1 cut(s) 119
NlaIV GGNNCC 3 cut(s) 233, 247, 389
NmeAIII GCCGAG 1 cut(s) 119
NmuCI GTSAC 1 cut(s) 776
PagI TCATGA 1 cut(s) 115
PfeI GAWTC 2 cut(s) 57, 135
PinAI ACCGGT 1 cut(s) 773
PkrI GCNGC 2 cut(s) 843, 871
PluTI GGCGCC 1 cut(s) 249
Psp1406I AACGTT 1 cut(s) 69
Psp6I CCWGG 3 cut(s) 306, 357, 856
PspEI GGTNACC 1 cut(s) 353
PspGI CCWGG 3 cut(s) 306, 357, 856
PspN4I GGNNCC 3 cut(s) 233, 247, 389
RsaI GTAC 4 cut(s) 17, 395, 459, 862
RsaNI GTAC 4 cut(s) 16, 394, 458, 861
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 2 cut(s) 842, 870
Sau3AI GATC 2 cut(s) 112, 466
ScrFI CCNGG 4 cut(s) 308, 359, 744, 858
SduI GDGCHC 1 cut(s) 551
SfaNI GCATC 3 cut(s) 419, 727, 771
SfoI GGCGCC 1 cut(s) 247
SgrAI CRCCGGYG 1 cut(s) 773
Sse9I AATT 4 cut(s) 336, 563, 757, 792
SspDI GGCGCC 1 cut(s) 245
SspMI CTAG 2 cut(s) 291, 446
StyD4I CCNGG 4 cut(s) 306, 357, 742, 856
TaaI ACNGT 6 cut(s) 15, 92, 127, 353, 370, 573
TaiI ACGT 3 cut(s) 72, 405, 757
TaqI TCGA 1 cut(s) 96
TasI AATT 4 cut(s) 336, 563, 757, 792
TatI WGTACW 1 cut(s) 457
TfiI GAWTC 2 cut(s) 57, 135
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 2 cut(s) 441, 492
TseFI GTSAC 1 cut(s) 776
TseI GCWGC 2 cut(s) 841, 869
Tsp45I GTSAC 1 cut(s) 776
TspDTI ATGAA 3 cut(s) 132, 456, 504
TspRI CASTG 2 cut(s) 441, 492
XapI RAATTY 1 cut(s) 563
XspI CTAG 2 cut(s) 291, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.