pycom06g16820

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008866
Physical Location & Seq
Forward (+)
20952750 .. 20953760
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g16820.1

Sequence Viewer

Length: 1011 bp
ATGTTGGTCGACCTTTCCCACAACCCTCTCCCTCTTTACTTCCCATGGCTCATGCTTCCTGACCACCACCGTCACCACCACCACCACCACAACGCCCGTAACGGCGTTGAAGCTTACGAAAACCAACACCTGAAGTGTTTCTTCAGCCTTTACGCGCACCAAGTCCACTGTCTCGAACTGCCCGAGGCTTTCGAAAAGACTGTATGTGGCTCGTCACCCGGTTGGCTAATCTTGCGCGACGAAACTACGTCGTTGGTCACCCTCCTCAACCCTCTCAACCGCGCCCAAATCGCTCTCCCAGAGCTCTCCTCCTCTCCGTTTTATGATCGAGACCCTTCAAGTGTTCTTCACAAAGCTGTGCTATCCTCAGATCCTTCTCGCTGTCCGTTTGATTACAAAGTTTTGGCGATTTTCGGAGAGAAGCGAAGGCTCATATGTTACAAAGCGAGGGAAAAGACGTGGACAATGCTACACGATGCAGGGAATAACTATGAAGACATTATTTTGTTTAATGGGGAGGAGTTGCTTGCCACTACTGAGATTGGAAAGCTCGTTTTATGCGATCTCGATGATGATGGCTGGTTTTCGCCGAGCGTTGATGAAATCGCTCCCCCTTTTACCTTCAAAGGTGGCAAAGTTTATCTTGTCGGCATTGGCGGAGAGCTCGTGATGCTGATAAGGTTTGTGCAGGACAACCCTCGTTACGGCCATGAGACTTATGACTTTGAGGCTTACAGGTATGAAAAGTTCGAAAGCGAATGGGAAGCCATCAACGGCTTAGGGGAATGGACGATTTTCGTGGGGCATAACCACACTACTGCCATTAAAGCTCAAGATTTCAAGGGCCTGATAAAACCTAACTGCATCTACTTCACTGACGAGAGTTTGGACATGGAAGATAGCTTGATGGTTGGCCACGATTCGGGAATCTTTAGCATGGAAGAAGGAAAATTTGAGGCGCTCGGATGTGATTTGAGTGGATTCACGTACGTTTGGCCTCGTCCGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

337

Amino Acids

38.46

Weight (kDa)

5.2

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 47 - 311 2.5e-46 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AccII CGCG 3 cut(s) 155, 237, 282
AciI CCGC 2 cut(s) 280, 657
AclWI GGATC 1 cut(s) 365
AcoI YGGCCR 2 cut(s) 706, 913
AcsI RAATTY 1 cut(s) 950
AcuI CTGAAG 2 cut(s) 127, 152
AfaI GTAC 1 cut(s) 989
AfiI CCNNNNNNNGG 2 cut(s) 704, 922
AgsI TTSAA 4 cut(s) 110, 339, 625, 841
AjiI CACGTC 1 cut(s) 459
AluBI AGCT 7 cut(s) 113, 304, 356, 550, 664, 830, 903
AluI AGCT 7 cut(s) 113, 304, 356, 550, 664, 830, 903
Alw21I GWGCWC 2 cut(s) 306, 666
Alw26I GTCTC 3 cut(s) 176, 324, 707
AlwI GGATC 1 cut(s) 365
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 4 cut(s) 706, 844, 913, 995
ApoI RAATTY 1 cut(s) 950
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 4 cut(s) 157, 237, 284, 961
AspS9I GGNCC 1 cut(s) 844
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 3 cut(s) 65, 207, 250
AsuII TTCGAA 2 cut(s) 192, 750
AvaI CYCGRG 1 cut(s) 182
BalI TGGCCA 1 cut(s) 915
BanII GRGCYC 2 cut(s) 306, 666
BauI CACGAG 1 cut(s) 665
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 2 cut(s) 306, 666
BccI CCATC 3 cut(s) 569, 776, 901
BceAI ACGGC 3 cut(s) 118, 721, 790
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 3 cut(s) 176, 324, 707
BfaI CTAG 1 cut(s) 1009
BfoI RGCGCY 1 cut(s) 962
Bme1390I CCNGG 1 cut(s) 219
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 459
BmgT120I GGNCC 1 cut(s) 844
BmrFI CCNGG 1 cut(s) 219
BmsI GCATC 3 cut(s) 466, 660, 873
BpiI GAAGAC 1 cut(s) 501
BplI GAGNNNNNCTC 2 cut(s) 293, 325
Bpu10I CCTNAGC 1 cut(s) 778
Bpu14I TTCGAA 2 cut(s) 192, 750
BpuEI CTTGAG 1 cut(s) 816
BpuMI CCSGG 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 987
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 44, 183
BsaWI WCCGGW 1 cut(s) 1003
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bsc4I CCNNNNNNNGG 2 cut(s) 704, 922
BseDI CCNNGG 2 cut(s) 44, 183
BseGI GGATG 1 cut(s) 971
BseLI CCNNNNNNNGG 2 cut(s) 704, 922
BseMII CTCAG 2 cut(s) 381, 528
BseRI GAGGAG 4 cut(s) 254, 298, 301, 533
BsgI GTGCAG 1 cut(s) 707
Bsh1236I CGCG 3 cut(s) 155, 237, 282
BshFI GGCC 4 cut(s) 708, 846, 915, 997
BsiHKAI GWGCWC 2 cut(s) 306, 666
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 219, 1004
BsiWI CGTACG 1 cut(s) 987
BslI CCNNNNNNNGG 2 cut(s) 704, 922
BsmAI GTCTC 3 cut(s) 176, 324, 707
BsnI GGCC 4 cut(s) 708, 846, 915, 997
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 182
Bsp119I TTCGAA 2 cut(s) 192, 750
Bsp1286I GDGCHC 2 cut(s) 306, 666
Bsp143I GATC 3 cut(s) 325, 370, 562
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 2 cut(s) 280, 657
BspANI GGCC 4 cut(s) 708, 846, 915, 997
BspCNI CTCAG 2 cut(s) 380, 529
BspFNI CGCG 3 cut(s) 155, 237, 282
BspPI GGATC 1 cut(s) 365
BspT104I TTCGAA 2 cut(s) 192, 750
BspTNI GGTCTC 1 cut(s) 324
BssECI CCNNGG 2 cut(s) 44, 183
BssMI GATC 3 cut(s) 325, 370, 562
BssSI CACGAG 1 cut(s) 665
BssT1I CCWWGG 1 cut(s) 44
Bst2BI CACGAG 1 cut(s) 665
Bst4CI ACNGT 3 cut(s) 71, 170, 202
BstBAI YACGTR 1 cut(s) 987
BstBI TTCGAA 2 cut(s) 192, 750
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 3 cut(s) 367, 537, 778
BstDSI CCRYGG 1 cut(s) 44
BstEII GGTNACC 1 cut(s) 256
BstF5I GGATG 1 cut(s) 971
BstFNI CGCG 3 cut(s) 155, 237, 282
BstH2I RGCGCY 1 cut(s) 962
BstHHI GCGC 4 cut(s) 157, 237, 284, 961
BstKTI GATC 3 cut(s) 328, 373, 565
BstMAI GTCTC 3 cut(s) 176, 324, 707
BstMBI GATC 3 cut(s) 325, 370, 562
BstMWI GCNNNNNNNGC 4 cut(s) 232, 290, 670, 827
BstPI GGTNACC 1 cut(s) 256
BstSCI CCNGG 1 cut(s) 217
BstUI CGCG 3 cut(s) 155, 237, 282
BstV2I GAAGAC 1 cut(s) 501
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 4 cut(s) 708, 846, 915, 997
BtgI CCRYGG 1 cut(s) 44
BtrI CACGTC 1 cut(s) 459
BtsCI GGATG 1 cut(s) 971
BtsIMutI CAGTG 2 cut(s) 166, 873
Cac8I GCNNGC 1 cut(s) 528
CfoI GCGC 4 cut(s) 157, 237, 284, 961
Cfr13I GGNCC 1 cut(s) 844
Csp6I GTAC 1 cut(s) 988
CviAII CATG 5 cut(s) 45, 52, 710, 892, 937
CviQI GTAC 1 cut(s) 988
DdeI CTNAG 3 cut(s) 367, 537, 778
DpnI GATC 3 cut(s) 327, 372, 564
DpnII GATC 3 cut(s) 325, 370, 562
EaeI YGGCCR 2 cut(s) 706, 913
EciI GGCGGA 1 cut(s) 672
Ecl136II GAGCTC 2 cut(s) 304, 664
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 2 cut(s) 306, 666
Eco31I GGTCTC 1 cut(s) 324
Eco53kI GAGCTC 2 cut(s) 304, 664
Eco57I CTGAAG 2 cut(s) 127, 152
Eco88I CYCGRG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 256
EcoICRI GAGCTC 2 cut(s) 304, 664
EcoO109I RGGNCCY 1 cut(s) 844
EcoO65I GGTNACC 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 44
EcoT38I GRGCYC 2 cut(s) 306, 666
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 5 cut(s) 48, 55, 713, 895, 940
FalI AAGNNNNNCTT 4 cut(s) 125, 157, 627, 659
FatI CATG 5 cut(s) 44, 51, 709, 891, 936
FauNDI CATATG 1 cut(s) 434
FblI GTMKAC 1 cut(s) 9
FokI GGATG 1 cut(s) 978
FriOI GRGCYC 2 cut(s) 306, 666
FspBI CTAG 1 cut(s) 1009
GlaI GCGC 4 cut(s) 156, 236, 283, 960
HaeII RGCGCY 1 cut(s) 962
HaeIII GGCC 4 cut(s) 708, 846, 915, 997
HapII CCGG 2 cut(s) 219, 1004
HhaI GCGC 4 cut(s) 157, 237, 284, 961
Hin1II CATG 5 cut(s) 48, 55, 713, 895, 940
Hin6I GCGC 4 cut(s) 155, 235, 282, 959
HinP1I GCGC 4 cut(s) 155, 235, 282, 959
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 3 cut(s) 920, 927, 981
HpaII CCGG 2 cut(s) 219, 1004
HphI GGTGA 3 cut(s) 65, 207, 250
Hpy166II GTNNAC 3 cut(s) 10, 166, 462
Hpy188I TCNGA 3 cut(s) 370, 416, 965
Hpy188III TCNNGA 7 cut(s) 59, 173, 329, 566, 667, 833, 924
Hpy8I GTNNAC 3 cut(s) 10, 166, 462
Hpy99I CGWCG 2 cut(s) 242, 253
HpyAV CCTTC 5 cut(s) 345, 384, 420, 631, 938
HpyCH4III ACNGT 3 cut(s) 71, 170, 202
HpyCH4IV ACGT 4 cut(s) 248, 458, 986, 990
HpyCH4V TGCA 3 cut(s) 479, 688, 864
HpyF10VI GCNNNNNNNGC 4 cut(s) 232, 290, 670, 827
HpyF3I CTNAG 3 cut(s) 367, 537, 778
HpySE526I ACGT 4 cut(s) 248, 458, 986, 990
Hsp92II CATG 5 cut(s) 48, 55, 713, 895, 940
HspAI GCGC 4 cut(s) 155, 235, 282, 959
Kzo9I GATC 3 cut(s) 325, 370, 562
LmnI GCTCC 1 cut(s) 613
LpnPI CCDG 9 cut(s) 72, 143, 232, 312, 465, 565, 674, 721, 860
LweI GCATC 3 cut(s) 466, 660, 873
MaeI CTAG 1 cut(s) 1009
MaeII ACGT 4 cut(s) 248, 458, 986, 990
MaeIII GTNAC 6 cut(s) 71, 98, 213, 256, 437, 701
MalI GATC 3 cut(s) 327, 372, 564
MboI GATC 3 cut(s) 325, 370, 562
MboII GAAGA 5 cut(s) 133, 338, 506, 908, 953
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 2 cut(s) 306, 666
MlsI TGGCCA 1 cut(s) 915
MluCI AATT 1 cut(s) 950
MluNI TGGCCA 1 cut(s) 915
Mox20I TGGCCA 1 cut(s) 915
MroXI GAANNNNTTC 1 cut(s) 137
MscI TGGCCA 1 cut(s) 915
MseI TTAA 2 cut(s) 510, 825
Msp20I TGGCCA 1 cut(s) 915
MspI CCGG 2 cut(s) 219, 1004
MspR9I CCNGG 1 cut(s) 219
MvnI CGCG 3 cut(s) 155, 237, 282
MwoI GCNNNNNNNGC 4 cut(s) 232, 290, 670, 827
NciI CCSGG 1 cut(s) 219
NcoI CCATGG 1 cut(s) 44
NdeI CATATG 1 cut(s) 434
NdeII GATC 3 cut(s) 325, 370, 562
NlaIII CATG 5 cut(s) 48, 55, 713, 895, 940
NmeAIII GCCGAG 1 cut(s) 615
NmuCI GTSAC 3 cut(s) 71, 213, 256
NspV TTCGAA 2 cut(s) 192, 750
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 558
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 3 cut(s) 920, 927, 981
Pfl23II CGTACG 1 cut(s) 987
Ppu21I YACGTR 1 cut(s) 987
Psp124BI GAGCTC 2 cut(s) 306, 666
PspEI GGTNACC 1 cut(s) 256
PspLI CGTACG 1 cut(s) 987
PspPI GGNCC 1 cut(s) 844
PsrI GAACNNNNNNTAC 2 cut(s) 731, 763
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 1 cut(s) 989
RsaNI GTAC 1 cut(s) 988
SacI GAGCTC 2 cut(s) 306, 666
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 510, 825
Sau3AI GATC 3 cut(s) 325, 370, 562
Sau96I GGNCC 1 cut(s) 844
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 2 cut(s) 306, 666
SfaNI GCATC 3 cut(s) 466, 660, 873
SfuI TTCGAA 2 cut(s) 192, 750
SmlI CTYRAG 1 cut(s) 831
SmoI CTYRAG 1 cut(s) 831
Sse9I AATT 1 cut(s) 950
SsiI CCGC 2 cut(s) 280, 657
SspMI CTAG 1 cut(s) 1009
SstI GAGCTC 2 cut(s) 306, 666
StyD4I CCNGG 1 cut(s) 217
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 3 cut(s) 71, 170, 202
TaiI ACGT 4 cut(s) 251, 461, 989, 993
TaqI TCGA 6 cut(s) 9, 174, 192, 328, 567, 750
TasI AATT 1 cut(s) 950
TfiI GAWTC 3 cut(s) 920, 927, 981
Tru1I TTAA 2 cut(s) 510, 825
Tru9I TTAA 2 cut(s) 510, 825
TscAI CASTG 2 cut(s) 173, 880
TseFI GTSAC 3 cut(s) 71, 213, 256
Tsp45I GTSAC 3 cut(s) 71, 213, 256
TspDTI ATGAA 3 cut(s) 507, 615, 756
TspGWI ACGGA 2 cut(s) 306, 375
TspRI CASTG 2 cut(s) 173, 880
XapI RAATTY 1 cut(s) 950
XmiI GTMKAC 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.