MD14G1196300.v1.1

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008866
Physical Location & Seq
Forward (+)
28670038 .. 28671063
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1196300.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGTTGGTCGACCTTGCCCACAATCCTCTCCCTCTCGACTTCCCATGGCTCATGCTGCCCGACCACCACCGTCACCACCACCACGGCGTTCATGACGGCGCTGAAGCTTACGAAAACCAAAACCTGAAGTGTTTCTTCAGCCTTTACACCCACCAAACCCACTGTCTCGTACTGCCCGAGGCTTTCGAAAAGACTGTGTGTGGCTCGTCATCGGGGTGGCTAATCTTGCGCGATGAAACCACGTCGTCTGTCGCCCTCCTCAACCCTCTCAACCGCGCCCAAATCGCTCTCCCACAGCTCTGCTCCTCTCCGTTTTACGATCAAGACCCTTCACGTGTTCTTCACAAAGCTGTGCTATCCGCAGACCCTTCCCGCTATCCGTTTGATTACAAAGTATTGGCGATTTTCGGAGAGAAGCGAAGGCTCGTATGCTACAAAGCGAGGGACAAGACATGGACAGTGCTACAAGATGCAGGGAATAACTATGAAGACATTATTTTGTTTAAGGGGGAGGAGTTGCTTGCCACTACTGAGATTGGAAAGCTCGTTTTGTGCGATCTTGATGACGATGGCTGTTTTTCGCCGAGAGTTGATGAAATCGCTCCCCCTTTTACCTTCAAAGGAGGCAAAGTTTATCTTGTCGGCATTGGTGGAGAGCTCGTGATGCTGATAAGATTTGTGCAGGACAACCCTCGTTACGGCCATGAGACTTATGACTTTGAGGCTTACAGGTATGATCAGTTTGAAGGCGAATGGGAACCCATCAATGGCTTAGGAGAGTGGACAATTTTCGTGGGGCATAACCACACTTCCGCCATTAAAGCTCAAGATTTCAAGGGGCTAATAAAACCCAACTGCATCTATTTCACTGACGATAGTTTGGACATGGAAGATAGCTTGATGGTTGGCCACGATTTGGGAGTCTTTAGCATGGAAGACAGAAAAGTTGAGCTGCTCGGATGTGATTTGAGTGGATTCATGTATGTTTGGCCGCGTCCGGTCTGGTTTATGCCAAGCATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

342

Amino Acids

38.92

Weight (kDa)

5.06

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 45 - 310 3.7e-47 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 916
AccI GTMKAC 1 cut(s) 9
AccII CGCG 3 cut(s) 231, 276, 994
AciI CCGC 5 cut(s) 274, 360, 373, 813, 992
AcoI YGGCCR 3 cut(s) 700, 907, 989
AcuI CTGAAG 3 cut(s) 121, 123, 146
AcvI CACGTG 1 cut(s) 335
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 3 cut(s) 698, 767, 916
AflIII ACRYGT 1 cut(s) 334
AgsI TTSAA 3 cut(s) 619, 746, 835
AjiI CACGTC 1 cut(s) 243
AluBI AGCT 8 cut(s) 107, 298, 350, 544, 658, 824, 897, 952
AluI AGCT 8 cut(s) 107, 298, 350, 544, 658, 824, 897, 952
Alw21I GWGCWC 1 cut(s) 660
Alw26I GTCTC 2 cut(s) 170, 701
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 3 cut(s) 700, 907, 989
ApeKI GCWGC 2 cut(s) 55, 952
ArsI GACNNNNNNTTYG 2 cut(s) 148, 180
Asp700I GAANNNNTTC 1 cut(s) 131
AspLEI GCGC 3 cut(s) 101, 231, 278
AsuHPI GGTGA 1 cut(s) 65
AsuII TTCGAA 1 cut(s) 186
AvaI CYCGRG 1 cut(s) 176
BalI TGGCCA 1 cut(s) 909
BanII GRGCYC 1 cut(s) 660
BauI CACGAG 1 cut(s) 659
BbrPI CACGTG 1 cut(s) 335
BbsI GAAGAC 2 cut(s) 495, 942
Bbv12I GWGCWC 1 cut(s) 660
BbvI GCAGC 2 cut(s) 42, 939
BccI CCATC 3 cut(s) 563, 770, 895
BceAI ACGGC 3 cut(s) 100, 112, 715
BclI TGATCA 1 cut(s) 736
BcoDI GTCTC 2 cut(s) 170, 701
BfoI RGCGCY 1 cut(s) 102
BisI GCNGC 3 cut(s) 56, 953, 992
BlsI GCNGC 3 cut(s) 57, 954, 993
BmeT110I CYCGRG 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 759
BmsI GCATC 3 cut(s) 460, 654, 867
BpiI GAAGAC 2 cut(s) 495, 942
Bpu10I CCTNAGC 1 cut(s) 772
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 810
BsaAI YACGTR 1 cut(s) 335
BsaJI CCNNGG 3 cut(s) 44, 82, 177
BsaWI WCCGGW 1 cut(s) 997
BsaXI ACNNNNNCTCC 4 cut(s) 615, 645, 645, 675
Bsc4I CCNNNNNNNGG 3 cut(s) 698, 767, 916
BseDI CCNNGG 3 cut(s) 44, 82, 177
BseGI GGATG 1 cut(s) 965
BseLI CCNNNNNNNGG 3 cut(s) 698, 767, 916
BseMII CTCAG 1 cut(s) 522
BseRI GAGGAG 3 cut(s) 248, 295, 527
BseXI GCAGC 2 cut(s) 42, 939
BsgI GTGCAG 1 cut(s) 701
Bsh1236I CGCG 3 cut(s) 231, 276, 994
BshFI GGCC 3 cut(s) 702, 909, 991
BsiHKAI GWGCWC 1 cut(s) 660
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 1 cut(s) 998
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 3 cut(s) 698, 767, 916
BsmAI GTCTC 2 cut(s) 170, 701
BsmFI GGGAC 1 cut(s) 458
BsnI GGCC 3 cut(s) 702, 909, 991
BsoBI CYCGRG 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 186
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 3 cut(s) 319, 556, 736
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 5 cut(s) 274, 360, 373, 813, 992
BspANI GGCC 3 cut(s) 702, 909, 991
BspCNI CTCAG 1 cut(s) 523
BspFNI CGCG 3 cut(s) 231, 276, 994
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 759
BspT104I TTCGAA 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 44, 82, 177
BssMI GATC 3 cut(s) 319, 556, 736
BssSI CACGAG 1 cut(s) 659
BssT1I CCWWGG 1 cut(s) 44
Bst2BI CACGAG 1 cut(s) 659
Bst4CI ACNGT 4 cut(s) 71, 164, 196, 460
BstBAI YACGTR 1 cut(s) 335
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 522
BstDEI CTNAG 2 cut(s) 531, 772
BstDSI CCRYGG 2 cut(s) 44, 82
BstF5I GGATG 1 cut(s) 965
BstFNI CGCG 3 cut(s) 231, 276, 994
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 3 cut(s) 101, 231, 278
BstKTI GATC 3 cut(s) 322, 559, 739
BstMAI GTCTC 2 cut(s) 170, 701
BstMBI GATC 3 cut(s) 319, 556, 736
BstMWI GCNNNNNNNGC 5 cut(s) 55, 226, 284, 664, 821
BstUI CGCG 3 cut(s) 231, 276, 994
BstV1I GCAGC 2 cut(s) 42, 939
BstV2I GAAGAC 2 cut(s) 495, 942
BsuRI GGCC 3 cut(s) 702, 909, 991
BtgI CCRYGG 2 cut(s) 44, 82
BtgZI GCGATG 1 cut(s) 246
BtrI CACGTC 1 cut(s) 243
BtsCI GGATG 1 cut(s) 965
BtsIMutI CAGTG 3 cut(s) 160, 465, 867
Cac8I GCNNGC 1 cut(s) 522
CciI TCATGA 1 cut(s) 91
CfoI GCGC 3 cut(s) 101, 231, 278
CseI GACGC 1 cut(s) 983
Csp6I GTAC 1 cut(s) 170
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviAII CATG 9 cut(s) 45, 52, 92, 453, 704, 886, 931, 979, 1018
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 2 cut(s) 531, 772
DpnI GATC 3 cut(s) 321, 558, 738
DpnII GATC 3 cut(s) 319, 556, 736
EaeI YGGCCR 3 cut(s) 700, 907, 989
EciI GGCGGA 1 cut(s) 802
Ecl136II GAGCTC 1 cut(s) 658
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 660
Eco53kI GAGCTC 1 cut(s) 658
Eco57I CTGAAG 3 cut(s) 121, 123, 146
Eco72I CACGTG 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 44
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 9 cut(s) 48, 55, 95, 456, 707, 889, 934, 982, 1021
FalI AAGNNNNNCTT 5 cut(s) 119, 151, 621, 653, 1006
FaqI GGGAC 1 cut(s) 458
FatI CATG 9 cut(s) 44, 51, 91, 452, 703, 885, 930, 978, 1017
FauI CCCGC 1 cut(s) 380
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 3 cut(s) 56, 953, 992
FokI GGATG 1 cut(s) 972
FriOI GRGCYC 1 cut(s) 660
Fsp4HI GCNGC 3 cut(s) 56, 953, 992
GlaI GCGC 3 cut(s) 100, 230, 277
GluI GCNGC 3 cut(s) 56, 953, 992
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 3 cut(s) 702, 909, 991
HapII CCGG 1 cut(s) 998
HgaI GACGC 1 cut(s) 983
HhaI GCGC 3 cut(s) 101, 231, 278
Hin1II CATG 9 cut(s) 48, 55, 95, 456, 707, 889, 934, 982, 1021
Hin6I GCGC 3 cut(s) 99, 229, 276
HinP1I GCGC 3 cut(s) 99, 229, 276
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 2 cut(s) 921, 975
HpaII CCGG 1 cut(s) 998
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 2 cut(s) 10, 783
Hpy188I TCNGA 2 cut(s) 410, 959
Hpy188III TCNNGA 6 cut(s) 35, 92, 323, 560, 661, 827
Hpy8I GTNNAC 2 cut(s) 10, 783
Hpy99I CGWCG 1 cut(s) 247
HpyAV CCTTC 5 cut(s) 339, 378, 414, 625, 740
HpyCH4III ACNGT 4 cut(s) 71, 164, 196, 460
HpyCH4IV ACGT 2 cut(s) 242, 334
HpyCH4V TGCA 3 cut(s) 473, 682, 858
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 226, 284, 664, 821
HpyF3I CTNAG 2 cut(s) 531, 772
HpySE526I ACGT 2 cut(s) 242, 334
Hsp92II CATG 9 cut(s) 48, 55, 95, 456, 707, 889, 934, 982, 1021
HspAI GCGC 3 cut(s) 99, 229, 276
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 3 cut(s) 319, 556, 736
LmnI GCTCC 2 cut(s) 308, 607
LpnPI CCDG 6 cut(s) 137, 459, 668, 715, 988, 1011
Lsp1109I GCAGC 2 cut(s) 42, 939
LweI GCATC 3 cut(s) 460, 654, 867
MaeII ACGT 2 cut(s) 242, 334
MaeIII GTNAC 2 cut(s) 71, 695
MalI GATC 3 cut(s) 321, 558, 738
MboI GATC 3 cut(s) 319, 556, 736
MboII GAAGA 5 cut(s) 127, 332, 500, 902, 947
MhlI GDGCHC 1 cut(s) 660
MlsI TGGCCA 1 cut(s) 909
MluCI AATT 1 cut(s) 786
MluNI TGGCCA 1 cut(s) 909
MlyI GAGTC 1 cut(s) 930
Mox20I TGGCCA 1 cut(s) 909
MroXI GAANNNNTTC 1 cut(s) 131
MscI TGGCCA 1 cut(s) 909
MseI TTAA 2 cut(s) 504, 819
MslI CAYNNNNRTG 1 cut(s) 214
Msp20I TGGCCA 1 cut(s) 909
MspI CCGG 1 cut(s) 998
MvnI CGCG 3 cut(s) 231, 276, 994
MwoI GCNNNNNNNGC 5 cut(s) 55, 226, 284, 664, 821
NcoI CCATGG 1 cut(s) 44
NdeII GATC 3 cut(s) 319, 556, 736
NlaIII CATG 9 cut(s) 48, 55, 95, 456, 707, 889, 934, 982, 1021
NlaIV GGNNCC 1 cut(s) 759
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 1 cut(s) 71
NspV TTCGAA 1 cut(s) 186
PagI TCATGA 1 cut(s) 91
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 552
PdmI GAANNNNTTC 1 cut(s) 131
PfeI GAWTC 1 cut(s) 975
PflMI CCANNNNNTGG 1 cut(s) 916
PkrI GCNGC 3 cut(s) 57, 954, 993
PleI GAGTC 1 cut(s) 929
PmaCI CACGTG 1 cut(s) 335
PmlI CACGTG 1 cut(s) 335
PpsI GAGTC 1 cut(s) 929
Ppu21I YACGTR 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 660
PspCI CACGTG 1 cut(s) 335
PspN4I GGNNCC 1 cut(s) 759
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
RseI CAYNNNNRTG 1 cut(s) 214
SacI GAGCTC 1 cut(s) 660
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 504, 819
SatI GCNGC 3 cut(s) 56, 953, 992
Sau3AI GATC 3 cut(s) 319, 556, 736
SchI GAGTC 1 cut(s) 930
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 3 cut(s) 460, 654, 867
SfuI TTCGAA 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 214
SmlI CTYRAG 1 cut(s) 825
SmoI CTYRAG 1 cut(s) 825
Sse9I AATT 1 cut(s) 786
SsiI CCGC 5 cut(s) 274, 360, 373, 813, 992
SstI GAGCTC 1 cut(s) 660
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 4 cut(s) 71, 164, 196, 460
TaiI ACGT 2 cut(s) 245, 337
TaqI TCGA 3 cut(s) 9, 36, 186
TasI AATT 1 cut(s) 786
TauI GCSGC 1 cut(s) 994
TfiI GAWTC 1 cut(s) 975
Tru1I TTAA 2 cut(s) 504, 819
Tru9I TTAA 2 cut(s) 504, 819
TscAI CASTG 3 cut(s) 167, 465, 874
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 2 cut(s) 55, 952
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 5 cut(s) 80, 249, 501, 609, 967
TspGWI ACGGA 2 cut(s) 300, 369
TspRI CASTG 3 cut(s) 167, 465, 874
Van91I CCANNNNNTGG 1 cut(s) 916
XmiI GTMKAC 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.