Rroxscaffold_3G00272580

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008866
Physical Location & Seq
Reverse (-)
64644580 .. 64645536
957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272580.1

Sequence Viewer

Length: 957 bp
ATGGTGAGCTACTCCCCATGGCTAATGGTTCCTAACCAGCACCACTCCGAGGATCGTCACCGCACTGAATTCTCCATTTCCAGTGGACTTGGTTGGTGGACTTATTTTGCAAATTCCATATGTGGAGAGGTTGAGGAAAAACGCATCAAACGATCTTTCCTCAACGTTTCCACCAACCAAGTCCACACTCTCGAACTACCCGAGGATGCTTGTGGAAAAAGAAAAACCATATGTGGATTCTCACTAGGTTGGCTAATCCTGTTCGACAATCGCAACTCATCATTGACTCTCCTCAACCCTTTCATCGGCGACCAAATCAACGACCTCCCACGACTCCATCGAAAGGGCACCGTCCACAAGGCCGTACTGTCTGCAGACCCGTCTTGCAACCCTAACGGCTACAAAGTCCTGGCAATCTTCGGAGACAAAAGAGAGCTAATTGGTTACGAACAAGGCAAGAAGCAATGGACTATGTTACAAGATTATGGAAATCACTACGACGATGTTCTTGTCCAAACGGACGAGGCGTTTGTAGCAGTGAGTGAGTTGGGGAAGCTTGTGTGCTGCAATCCACCAGCTTGTAACGAACATGTACCAGCTTCTTTCTTTAAAGGTAAAAAGGTTTATTTAGTGAGCATAGATGAAGACGTATTTATGCTGGTAAGCTTTTCGGGGACGAGCTACTGTTTTTGGCATGAAACCTACGACGACAAGCTTAAAAGGTTTGAGGTTTACAGGTATGATGAAGGACGGTGGTGCCACACCGAAAATCTTCACGAGTTTACGATTTTCTTGGGACAGAACCACTCAGTAGCTGTGCGTAATGCCCCGGATTTTAGACCTGATTGCATATACTTCACTGATGATGATGAAGGTAAAGTTGAAAACTCGGGCGTGTTTAGTTTGAAAGATGGCGAAGCTGAAAGAATGCAATGGTTTATGCCTAGGTTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

318

Amino Acids

36.66

Weight (kDa)

5.65

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 53 - 300 2.7e-38 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 347, 756
AciI CCGC 1 cut(s) 61
AclI AACGTT 1 cut(s) 165
AclWI GGATC 1 cut(s) 60
AcsI RAATTY 2 cut(s) 68, 112
AfaI GTAC 2 cut(s) 366, 594
AfiI CCNNNNNNNGG 3 cut(s) 49, 305, 343
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 2 cut(s) 884, 907
AjnI CCWGG 1 cut(s) 408
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 1 cut(s) 60
AlwNI CAGNNNCTG 1 cut(s) 815
Ama87I CYCGRG 2 cut(s) 200, 889
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 1 cut(s) 564
ApoI RAATTY 2 cut(s) 68, 112
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
Asp700I GAANNNNTTC 1 cut(s) 771
AspA2I CCTAGG 1 cut(s) 944
AsuC2I CCSGG 1 cut(s) 830
AsuHPI GGTGA 2 cut(s) 16, 50
AvaI CYCGRG 2 cut(s) 200, 889
AvrII CCTAGG 1 cut(s) 944
BaeGI GKGCMC 1 cut(s) 350
BanI GGYRCC 2 cut(s) 347, 756
BauI CACGAG 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 551
BccI CCATC 2 cut(s) 345, 905
BceAI ACGGC 2 cut(s) 347, 412
BciT130I CCWGG 1 cut(s) 410
BcnI CCSGG 1 cut(s) 830
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 2 cut(s) 245, 945
BfmI CTRYAG 1 cut(s) 372
BisI GCNGC 1 cut(s) 565
BlnI CCTAGG 1 cut(s) 944
BlsI GCNGC 1 cut(s) 566
Bme1390I CCNGG 2 cut(s) 410, 830
BmeT110I CYCGRG 2 cut(s) 200, 889
BmiI GGNNCC 3 cut(s) 30, 349, 758
BmrFI CCNGG 2 cut(s) 410, 830
BmsI GCATC 2 cut(s) 153, 196
BpiI GAAGAC 1 cut(s) 651
BpuMI CCSGG 1 cut(s) 830
BsaJI CCNNGG 5 cut(s) 17, 48, 201, 828, 944
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 305, 343
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 2 cut(s) 470, 938
BseBI CCWGG 1 cut(s) 410
BseDI CCNNGG 5 cut(s) 17, 48, 201, 828, 944
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 3 cut(s) 49, 305, 343
BseMI GCAATG 2 cut(s) 470, 938
BseMII CTCAG 1 cut(s) 822
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 1 cut(s) 551
BshFI GGCC 1 cut(s) 362
BshNI GGYRCC 2 cut(s) 347, 756
BsiHKCI CYCGRG 2 cut(s) 200, 889
BsiSI CCGG 1 cut(s) 830
BslFI GGGAC 2 cut(s) 688, 810
BslI CCNNNNNNNGG 3 cut(s) 49, 305, 343
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 2 cut(s) 688, 810
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 1 cut(s) 362
BsoBI CYCGRG 2 cut(s) 200, 889
Bsp1286I GDGCHC 1 cut(s) 350
Bsp143I GATC 2 cut(s) 52, 152
Bsp19I CCATGG 1 cut(s) 17
BspACI CCGC 1 cut(s) 61
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 1 cut(s) 821
BspLI GGNNCC 3 cut(s) 30, 349, 758
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 1 cut(s) 60
BspT107I GGYRCC 2 cut(s) 347, 756
BsrDI GCAATG 2 cut(s) 470, 938
BsrI ACTGG 1 cut(s) 81
BssECI CCNNGG 5 cut(s) 17, 48, 201, 828, 944
BssMI GATC 2 cut(s) 52, 152
BssSI CACGAG 1 cut(s) 776
BssT1I CCWWGG 2 cut(s) 17, 944
Bst2BI CACGAG 1 cut(s) 776
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 4 cut(s) 352, 369, 686, 753
BstDEI CTNAG 1 cut(s) 808
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 1 cut(s) 211
BstKTI GATC 2 cut(s) 55, 155
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 2 cut(s) 52, 152
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 1 cut(s) 410
BstNSI RCATGY 1 cut(s) 593
BstSCI CCNGG 2 cut(s) 408, 828
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 350
BstV1I GCAGC 1 cut(s) 551
BstV2I GAAGAC 1 cut(s) 651
BsuRI GGCC 1 cut(s) 362
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 1 cut(s) 211
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 4 cut(s) 63, 88, 543, 858
CaiI CAGNNNCTG 1 cut(s) 815
Csp6I GTAC 2 cut(s) 365, 593
CviAII CATG 3 cut(s) 18, 590, 695
CviQI GTAC 2 cut(s) 365, 593
DdeI CTNAG 1 cut(s) 808
DpnI GATC 2 cut(s) 54, 154
DpnII GATC 2 cut(s) 52, 152
DraI TTTAAA 1 cut(s) 610
Eco130I CCWWGG 2 cut(s) 17, 944
Eco88I CYCGRG 2 cut(s) 200, 889
EcoRI GAATTC 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 408
EcoT14I CCWWGG 2 cut(s) 17, 944
ErhI CCWWGG 2 cut(s) 17, 944
FaeI CATG 3 cut(s) 21, 593, 698
FaqI GGGAC 2 cut(s) 688, 810
FatI CATG 3 cut(s) 17, 589, 694
FauNDI CATATG 2 cut(s) 119, 230
Fnu4HI GCNGC 1 cut(s) 565
FokI GGATG 1 cut(s) 218
Fsp4HI GCNGC 1 cut(s) 565
FspBI CTAG 2 cut(s) 245, 945
GluI GCNGC 1 cut(s) 565
HaeIII GGCC 1 cut(s) 362
HapII CCGG 1 cut(s) 830
Hin1II CATG 3 cut(s) 21, 593, 698
HindIII AAGCTT 3 cut(s) 554, 664, 713
HinfI GANTC 3 cut(s) 237, 286, 333
HpaII CCGG 1 cut(s) 830
HphI GGTGA 2 cut(s) 16, 50
Hpy166II GTNNAC 6 cut(s) 86, 99, 184, 355, 733, 783
Hpy188I TCNGA 2 cut(s) 49, 422
Hpy188III TCNNGA 2 cut(s) 191, 776
Hpy8I GTNNAC 6 cut(s) 86, 99, 184, 355, 733, 783
Hpy99I CGWCG 2 cut(s) 503, 710
HpyAV CCTTC 2 cut(s) 740, 866
HpyCH4III ACNGT 4 cut(s) 352, 369, 686, 753
HpyCH4IV ACGT 2 cut(s) 165, 648
HpyCH4V TGCA 6 cut(s) 110, 374, 387, 567, 849, 931
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpyF3I CTNAG 1 cut(s) 808
HpySE526I ACGT 2 cut(s) 165, 648
Hsp92II CATG 3 cut(s) 21, 593, 698
Kzo9I GATC 2 cut(s) 52, 152
Lsp1109I GCAGC 1 cut(s) 551
LweI GCATC 2 cut(s) 153, 196
MaeI CTAG 2 cut(s) 245, 945
MaeII ACGT 2 cut(s) 165, 648
MaeIII GTNAC 4 cut(s) 56, 443, 474, 581
MalI GATC 2 cut(s) 54, 154
MboI GATC 2 cut(s) 52, 152
MboII GAAGA 3 cut(s) 409, 656, 764
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 3 cut(s) 68, 112, 438
MlyI GAGTC 2 cut(s) 280, 327
MnlI CCTC 9 cut(s) 43, 121, 127, 170, 196, 302, 335, 517, 721
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 2 cut(s) 609, 717
MspI CCGG 1 cut(s) 830
MspR9I CCNGG 2 cut(s) 410, 830
Mva1269I GAATGC 1 cut(s) 933
MvaI CCWGG 1 cut(s) 410
MwoI GCNNNNNNNGC 1 cut(s) 533
NciI CCSGG 1 cut(s) 830
NcoI CCATGG 1 cut(s) 17
NdeI CATATG 2 cut(s) 119, 230
NdeII GATC 2 cut(s) 52, 152
NlaIII CATG 3 cut(s) 21, 593, 698
NlaIV GGNNCC 3 cut(s) 30, 349, 758
NmuCI GTSAC 1 cut(s) 56
NspI RCATGY 1 cut(s) 593
PciI ACATGT 1 cut(s) 589
PcsI WCGNNNNNNNCGW 4 cut(s) 148, 198, 337, 524
PctI GAATGC 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 771
PfeI GAWTC 1 cut(s) 237
PkrI GCNGC 1 cut(s) 566
PleI GAGTC 2 cut(s) 280, 327
PpsI GAGTC 2 cut(s) 280, 327
PscI ACATGT 1 cut(s) 589
Psp1406I AACGTT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 408
PspGI CCWGG 1 cut(s) 408
PspN4I GGNNCC 3 cut(s) 30, 349, 758
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 1 cut(s) 815
RsaI GTAC 2 cut(s) 366, 594
RsaNI GTAC 2 cut(s) 365, 593
SaqAI TTAA 2 cut(s) 609, 717
SatI GCNGC 1 cut(s) 565
Sau3AI GATC 2 cut(s) 52, 152
SchI GAGTC 2 cut(s) 280, 327
ScrFI CCNGG 2 cut(s) 410, 830
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 2 cut(s) 153, 196
SfcI CTRYAG 1 cut(s) 372
Sse9I AATT 3 cut(s) 68, 112, 438
SsiI CCGC 1 cut(s) 61
SspMI CTAG 2 cut(s) 245, 945
StyD4I CCNGG 2 cut(s) 408, 828
StyI CCWWGG 2 cut(s) 17, 944
TaaI ACNGT 4 cut(s) 352, 369, 686, 753
TaiI ACGT 2 cut(s) 168, 651
TaqI TCGA 3 cut(s) 192, 264, 340
TasI AATT 3 cut(s) 68, 112, 438
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 2 cut(s) 609, 717
Tru9I TTAA 2 cut(s) 609, 717
TscAI CASTG 4 cut(s) 70, 88, 543, 865
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 1 cut(s) 564
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 5 cut(s) 292, 657, 711, 759, 885
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 4 cut(s) 70, 88, 543, 865
XapI RAATTY 2 cut(s) 68, 112
XceI RCATGY 1 cut(s) 593
XmaJI CCTAGG 1 cut(s) 944
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 2 cut(s) 245, 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.