MD06G1203000.v1.1

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005458
Physical Location & Seq
Reverse (-)
33748577 .. 33749548
972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1203000.v1.1.491

Sequence Viewer

Length: 828 bp
ATGCTTGAAGGACTAGCAAAAGCTCCAATCTCTCACACACAGCCTCATCGAGAATTGAGTCATCTGGTTATTGTTTCATGTGACATACCAGAGTCGAAGACTCTGGTAAAGCAGATGCCGAATGCCCTTCAAGGCAATGGTGCCGAAGATTCCAACCACAGCGAAGGTGAAGATCAATGGAATCCTGCAGGTAAGCTTGACAGCATGGAATCTGGTCTTACATCGGAAATCAATCGTATGAAATTGAAGAACATTGAAAACTTAGTTCTGAAGCTTAGACGATTGAACTATAATCACGATGAAGCAAGAACATACTACATTGCATCGCTCTGCGAGAACACAAATCCAGACCACAGATACATTTCTGAGATATTGTTAGCTTCAGGCCTCCTACTCAGAGACCTCGGCTCCAGCTTGACAACATTTCAGCTCCATCCCTCAGGCCATCCCATCAACCCTGAGTTGTTCTATGTTTTGGAGCAAACCAAGGCAAGCAGTTTGCTTGCAAAAGAAGAATGCATCCCTGATAAGGTAACCCTTGCAAAGCAAGAGAAAGAGAAATCTCATCGAAAACTCATATTTGACGCTGTCAATGAGATTGTTGTTCACAAGTTGGATTCAGCTGGTATCTCTCCCGTCCACGGCGGAGCCAGCATGGGATCACTGGTTGTCCTTGACTCCAATAACTTCAAAAATCTCATGTATATTTGTTTGTATATTGTATTTGACGCGTATAAACAGCTAAAACAAACTAAATTACAACCTTATTTTCCTATATGTCTATTTTCTTCTATCACATTTGTCTTCCTTTTCTCTCCTCTTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

276

Amino Acids

30.81

Weight (kDa)

6.26

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 117 - 207 3.4e-18 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 140
AccII CGCG 1 cut(s) 731
AciI CCGC 1 cut(s) 645
AclWI GGATC 1 cut(s) 667
AcuI CTGAAG 2 cut(s) 290, 366
AfiI CCNNNNNNNGG 2 cut(s) 529, 641
AflIII ACRYGT 1 cut(s) 729
AgsI TTSAA 6 cut(s) 8, 131, 247, 257, 286, 691
AluBI AGCT 8 cut(s) 23, 196, 274, 380, 414, 430, 623, 742
AluI AGCT 8 cut(s) 23, 196, 274, 380, 414, 430, 623, 742
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 1 cut(s) 667
AoxI GGCC 2 cut(s) 385, 442
AsuHPI GGTGA 1 cut(s) 179
AxyI CCTNAGG 1 cut(s) 439
BanI GGYRCC 1 cut(s) 140
BbsI GAAGAC 2 cut(s) 104, 796
BccI CCATC 3 cut(s) 441, 453, 458
BceAI ACGGC 1 cut(s) 658
BcoDI GTCTC 1 cut(s) 393
BfaI CTAG 1 cut(s) 14
BfmI CTRYAG 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 179
BmiI GGNNCC 3 cut(s) 142, 409, 649
BmsI GCATC 3 cut(s) 105, 332, 528
BpiI GAAGAC 2 cut(s) 104, 796
BpmI CTGGAG 1 cut(s) 394
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 403, 486, 640
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 641
Bse1I ACTGG 1 cut(s) 669
Bse21I CCTNAGG 1 cut(s) 439
Bse3DI GCAATG 2 cut(s) 142, 318
BseDI CCNNGG 3 cut(s) 403, 486, 640
BseGI GGATG 3 cut(s) 433, 445, 519
BseLI CCNNNNNNNGG 2 cut(s) 529, 641
BseMI GCAATG 2 cut(s) 142, 318
BseMII CTCAG 4 cut(s) 357, 409, 450, 453
BseNI ACTGG 1 cut(s) 669
BseRI GAGGAG 1 cut(s) 807
Bsh1236I CGCG 1 cut(s) 731
BshFI GGCC 2 cut(s) 387, 444
BshNI GGYRCC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 529, 641
BsmAI GTCTC 1 cut(s) 393
BsmI GAATGC 2 cut(s) 127, 521
BsnI GGCC 2 cut(s) 387, 444
Bso31I GGTCTC 1 cut(s) 393
Bsp143I GATC 2 cut(s) 172, 659
BspACI CCGC 1 cut(s) 645
BspANI GGCC 2 cut(s) 387, 444
BspCNI CTCAG 4 cut(s) 358, 408, 451, 452
BspFNI CGCG 1 cut(s) 731
BspLI GGNNCC 3 cut(s) 142, 409, 649
BspMAI CTGCAG 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 1 cut(s) 667
BspT107I GGYRCC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 393
BsrDI GCAATG 2 cut(s) 142, 318
BsrI ACTGG 1 cut(s) 669
BssECI CCNNGG 3 cut(s) 403, 486, 640
BssMI GATC 2 cut(s) 172, 659
BssT1I CCWWGG 1 cut(s) 486
BstC8I GCNNGC 3 cut(s) 493, 504, 652
BstDEI CTNAG 6 cut(s) 262, 275, 366, 395, 439, 459
BstDSI CCRYGG 1 cut(s) 640
BstEII GGTNACC 1 cut(s) 532
BstF5I GGATG 3 cut(s) 433, 445, 519
BstFNI CGCG 1 cut(s) 731
BstKTI GATC 2 cut(s) 175, 662
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 2 cut(s) 172, 659
BstMWI GCNNNNNNNGC 1 cut(s) 651
BstPI GGTNACC 1 cut(s) 532
BstSFI CTRYAG 1 cut(s) 186
BstUI CGCG 1 cut(s) 731
BstV2I GAAGAC 2 cut(s) 104, 796
Bsu36I CCTNAGG 1 cut(s) 439
BsuRI GGCC 2 cut(s) 387, 444
BtgI CCRYGG 1 cut(s) 640
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 3 cut(s) 433, 445, 519
BtsIMutI CAGTG 1 cut(s) 662
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 3 cut(s) 493, 504, 652
CseI GACGC 2 cut(s) 593, 737
CviAII CATG 5 cut(s) 78, 205, 655, 700, 825
DdeI CTNAG 6 cut(s) 262, 275, 366, 395, 439, 459
DpnI GATC 2 cut(s) 174, 661
DpnII GATC 2 cut(s) 172, 659
EciI GGCGGA 1 cut(s) 660
Eco130I CCWWGG 1 cut(s) 486
Eco147I AGGCCT 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 393
Eco57I CTGAAG 2 cut(s) 290, 366
Eco81I CCTNAGG 1 cut(s) 439
Eco91I GGTNACC 1 cut(s) 532
EcoO65I GGTNACC 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 486
EcoT22I ATGCAT 1 cut(s) 521
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 5 cut(s) 81, 208, 658, 703, 828
FatI CATG 5 cut(s) 77, 204, 654, 699, 824
FokI GGATG 3 cut(s) 420, 432, 506
FspBI CTAG 1 cut(s) 14
GsuI CTGGAG 1 cut(s) 394
HaeIII GGCC 2 cut(s) 387, 444
HgaI GACGC 2 cut(s) 593, 737
Hin1II CATG 5 cut(s) 81, 208, 658, 703, 828
HindIII AAGCTT 2 cut(s) 194, 272
HinfI GANTC 8 cut(s) 58, 92, 100, 149, 181, 209, 617, 677
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 2 cut(s) 607, 640
Hpy188I TCNGA 4 cut(s) 226, 270, 367, 398
Hpy188III TCNNGA 3 cut(s) 50, 296, 347
Hpy8I GTNNAC 2 cut(s) 607, 640
HpyAV CCTTC 2 cut(s) 137, 158
HpyCH4V TGCA 6 cut(s) 188, 323, 506, 519, 542, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 651
HpyF3I CTNAG 6 cut(s) 262, 275, 366, 395, 439, 459
Hsp92II CATG 5 cut(s) 81, 208, 658, 703, 828
Kzo9I GATC 2 cut(s) 172, 659
LmnI GCTCC 5 cut(s) 28, 413, 435, 478, 647
LweI GCATC 3 cut(s) 105, 332, 528
MaeI CTAG 1 cut(s) 14
MaeIII GTNAC 2 cut(s) 80, 532
MalI GATC 2 cut(s) 174, 661
MboI GATC 2 cut(s) 172, 659
MboII GAAGA 7 cut(s) 109, 158, 182, 259, 524, 780, 796
MluCI AATT 3 cut(s) 53, 242, 755
MluI ACGCGT 1 cut(s) 729
MlyI GAGTC 4 cut(s) 67, 94, 101, 671
MmeI TCCRAC 2 cut(s) 177, 594
MnlI CCTC 5 cut(s) 54, 398, 413, 448, 828
Mph1103I ATGCAT 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 623
Mva1269I GAATGC 2 cut(s) 127, 521
MvnI CGCG 1 cut(s) 731
MwoI GCNNNNNNNGC 1 cut(s) 651
NdeII GATC 2 cut(s) 172, 659
NlaIII CATG 5 cut(s) 81, 208, 658, 703, 828
NlaIV GGNNCC 3 cut(s) 142, 409, 649
NmeAIII GCCGAG 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 80
NsiI ATGCAT 1 cut(s) 521
PceI AGGCCT 1 cut(s) 387
PctI GAATGC 2 cut(s) 127, 521
PfeI GAWTC 4 cut(s) 149, 181, 209, 617
PflFI GACNNNGTC 1 cut(s) 587
PleI GAGTC 4 cut(s) 66, 94, 100, 671
PpsI GAGTC 4 cut(s) 66, 94, 100, 671
PspEI GGTNACC 1 cut(s) 532
PspN4I GGNNCC 3 cut(s) 142, 409, 649
PstI CTGCAG 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 623
Sau3AI GATC 2 cut(s) 172, 659
SbfI CCTGCAGG 1 cut(s) 190
SchI GAGTC 4 cut(s) 67, 94, 101, 671
SdaI CCTGCAGG 1 cut(s) 190
SfaNI GCATC 3 cut(s) 105, 332, 528
SfcI CTRYAG 1 cut(s) 186
Sse8387I CCTGCAGG 1 cut(s) 190
Sse9I AATT 3 cut(s) 53, 242, 755
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 1 cut(s) 645
SspMI CTAG 1 cut(s) 14
StuI AGGCCT 1 cut(s) 387
StyI CCWWGG 1 cut(s) 486
TaqI TCGA 3 cut(s) 49, 95, 568
TasI AATT 3 cut(s) 53, 242, 755
TfiI GAWTC 4 cut(s) 149, 181, 209, 617
TscAI CASTG 1 cut(s) 669
TseFI GTSAC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 3 cut(s) 66, 254, 315
TspRI CASTG 1 cut(s) 669
Tth111I GACNNNGTC 1 cut(s) 587
XspI CTAG 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.