MD06G1211200.v1.1

Component of the post-replicative DNA mismatch repair system (MMR)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006881
Physical Location & Seq
Forward (+)
34388954 .. 34394363
5410 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1211200.v1.1.491

Sequence Viewer

Length: 2829 bp
ATGGATGCCAATTTTGAGGACCACAGCAAGCTTCCCGAGCTCAAGCTAGATGCAAAGCAGTCTCAAGGGTTTCTCTCATTCTTCAAAACCCTCCCCAATGATTCAAGAGCTATTCGATTGTTTGATCGTCGGGACTACTATACTGCTCATGGTGAAAATGCCACTTTCATCGCAAAGACCTACTACCGTACCACCACTGCTTTACGGCAGTTGGGTAGCGGATCTAATGGCCTATCCAGTGTGAGTGTTAGTAAAAATATGTTTGAGACAATTACGCGTGATATACTACTGGAAAGAACAGACCATACTCTTGAGATTTACGAGGGTAGTGGCTCAAGTTGGAAGCTGGTGAAAAGTGGAACGCCTGGAAACCTTGGCAGTTTCGAAGATGTTTTGTTTGCCAACAATGAGATGCAGGATACCCCAGTGGTTGTTGCGCTATTACCTAACTTTCGGGAAAATGGTTGCACAGTTGGGTTAGGATATGTTGATCTAACTAAACGAGTACTTGGATTAGCTGAATTTATTGATGATAGTCACTTTACAAATGTGGAATCAGCTTTAGTTGCACTTGGTTGCAAGGAATGCCTTCTGCCTTTAGAGAGTGGCAAAACTAGCGAAATTAGAACATTGCATGATGCATTGAGTAGATGTGGTGTGATGTTAACCGAGAGGAAGAAGACTGAATTTAAGATGAGGGATTTGGTTCAGGATCTCGGTCGGCTTGTCAAAGGCTCCATTGAACCAGTTCGAGATTTTGTGTCAGGGTTTGAGTTTGCACCTGGTGCATTGGGGGCATTGCTCTCTTATGCAGAATTACTTGCTGACGAGAGCAATTATGGAAATTATAGCATTCAAAGATATAATCTTGATAGCTATATGAGGTTAGATTCTGCTGCTATGAGGGCACTTAATGTCCTGGAAAGCAAAACTGATGCAAACAAAAATTTCAGTTTGTTTGGTCTTATGAATAGGACTTGTACTGCTGGAATGGGTAAAAGATTACTGCACATGTGGCTAAAACAGCCTTTATTGGATGTAAATGAAATCAACTCGAGGCTAGATTTGGTACAAGCATTTGTGGAGGATCCTGCACTTCGCCAAGATTTGAGGCAGCATCTGAAAAGAATTTCAGACATCGAGCGGCTGATGCACAATCTTGAGAAGAAAAGAGCTGGTTTGCAGCATATTGTGAAACTTTATCAGTCGTGTATAAGACTTCCCTACATTAAGAGTGCCCTAGAACGCTATGATGGACAGTTCTCCTCACTGACCAAGGAAAGGTATTGGGAACCCCTTGAGTTATGGACTGATGATCGCCACTTAAATAAGTTCATTGCTCTGGTGGAAGCTGCTGTTGACCTTGATCAACTTGAGAATGGAGAGTACATGATATCATCTGGTTATGACCCAGCACTGTCTGCACTGAACGAAGAGCAAGAGTCGTTGGAGCACCAAATACAGAATTTGCACAAACAAACTGCCAATGATCTCGATCTCGCCTTAGATAAGGCTTTAAAATTAGATAAAGGTACACAATTTGGACATGTTTTCAGAATCACAAAGAAGGAAGAGCCAAAAATACGGAAGAAGCTCACCACACAATTCATTGTCCTGGAAACTCGAAAGGATGGAGTGAAATTTACCAACACAAAGCTTAAAAAGTTAGGGGATCAGTATCAAAGAATAGTTGAAGAGTATAAGAGTTGTCAGAAAGAGCTTGTTAACCGAGTTATTCAAACTACAACAACTTTCTCTGAGGTGTTCTGGTCTGTAGCTGGGTTGCTCTCTGAATTGGATGTCTTACTTAGTTTTGCTGATTTGGCCTCTAGTTGTCCTACTCCTTACACTAGGCCAGTCATCACGCCACCGGATGAAGGGGATATTATATTAGAAGGCAGTAGACATCCCTGTGTGGAGGCTCAGGACTGGGTGAATTTTATACCCAACGATTGTAAACTTGTGAGAGGAAAGAGTTGGTTCCAAATCATCACAGGACCCAATATGGGTGGTAAATCAACATTTATCCGACAGGTTGGTGTGAATATTCTGATGGCACAAGTTGGTTGTTTCGTTCCTTGTGACTCAGCTAGCATTTCTGTTCGTGATTGCATTTTTGCTCGGGTGGGAGCTGGCGACTGCCAACTACGTGGAGTTTCTACCTTTATGCAAGAAATGCTTGAGACTGCATCTATACTGAAAGGAGCTACAGACAAGTCACTAATAATTATTGACGAGTTAGGCCGAGGGACTTCAACTTATGATGGATTCGGTTTAGCTTGGGCCATTTGTGAGCACCTTGTTGAAGTGATTAAAGCACCTACCTTATTTGCAACCCACTTTCATGAATTAACTGCTTTAGCTCATGAAAATGTCGTTGAGGACACTAATATGAAGCAAATTGTTGGCGTAGCAAACTATCATGTTAGTGCACATATTGACTCATCAAGCCGCAAGTTGACTATGCTGTACAAGGTTGAGCCAGGGGCTTGTGATCAAAGTTTCGGTATTCAAGTTGCAGAGTTTGCAAACTTTCCTGAAAGTGTTGTTTCCCTTGCTCGAGAAAAAGCTGCTGAACTGGAGGATTTCTCTGCTACTACAGTCACGCCAAATGATGCTACAGAAGAGGTTGGTTTGAAGCGTAAGAGAGAGCACGACACTGGTGATACGACTAAAGGAGCTGCTCGGGCGCACAAGTTTCTGGAGGAGTTTTCTAATCTGCCATTGGAGACGATGGATTTAAAGCAGGCTCTGCAAAGAGTTTGCAAGATGAAGGATGAGTTGCAAAAGGATGCAGCTAATTCCCAATGGCTCCAGCAGTTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000217 GO:0000400 GO:0000403 GO:0000404 GO:0000406 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006290 GO:0006298 GO:0006301 GO:0006310 GO:0006311 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0008094 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010520 GO:0010564 GO:0010605 GO:0010639 GO:0010948 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0030983 GO:0031323 GO:0031324 GO:0032135 GO:0032137 GO:0032138 GO:0032300 GO:0032301 GO:0032302 GO:0032991 GO:0033043 GO:0033554 GO:0034641 GO:0035822 GO:0040020 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045128 GO:0045786 GO:0045835 GO:0045910 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051321 GO:0051445 GO:0051447 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0060255 GO:0060631 GO:0061982 GO:0065007 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090304 GO:0097159 GO:0110029 GO:1901360 GO:1901363 GO:1903046 GO:1990391 GO:2000241 GO:2000242
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
MutS_I MutS_II MutS_III MutS_IV MutS_V

Protein Analysis

943

Amino Acids

105.83

Weight (kDa)

5.72

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MutS_I PF01624 23 - 128 7.2e-16 MutS domain I
MutS_II PF05188 152 - 283 2.6e-14 MutS domain II
MutS_III PF05192 299 - 606 1.4e-38 MutS domain III
MutS_IV PF05190 468 - 565 1.1e-19 MutS family domain IV
MutS_V PF00488 662 - 861 2.3e-78 MutS domain V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1144
AccI GTMKAC 1 cut(s) 1903
AccII CGCG 1 cut(s) 277
AciI CCGC 3 cut(s) 219, 1144, 2452
AclWI GGATC 5 cut(s) 229, 720, 1082, 1095, 1680
AcsI RAATTY 7 cut(s) 521, 686, 946, 1128, 1465, 1640, 1936
AdeI CACNNNGTG 1 cut(s) 785
AfaI GTAC 7 cut(s) 190, 507, 982, 1071, 1388, 1534, 2471
AfiI CCNNNNNNNGG 3 cut(s) 1281, 1877, 2006
AflIII ACRYGT 3 cut(s) 275, 1011, 1546
AjnI CCWGG 5 cut(s) 364, 781, 918, 1614, 2482
Alw21I GWGCWC 5 cut(s) 42, 1455, 2298, 2434, 2655
Alw26I GTCTC 4 cut(s) 66, 260, 2177, 2723
Alw44I GTGCAC 1 cut(s) 2430
AlwI GGATC 5 cut(s) 229, 720, 1082, 1095, 1680
AlwNI CAGNNNCTG 2 cut(s) 1120, 2752
Ama87I CYCGRG 5 cut(s) 35, 1054, 2121, 2559, 2685
AoxI GGCC 5 cut(s) 229, 1824, 1853, 2242, 2283
ApaLI GTGCAC 1 cut(s) 2430
ApeKI GCWGC 7 cut(s) 896, 1114, 1183, 1352, 2570, 2681, 2795
ApoI RAATTY 7 cut(s) 521, 686, 946, 1128, 1465, 1640, 1936
Asp700I GAANNNNTTC 2 cut(s) 588, 747
AspLEI GCGC 2 cut(s) 439, 2692
AspS9I GGNCC 3 cut(s) 19, 1997, 2283
AsuHPI GGTGA 5 cut(s) 164, 361, 1588, 1945, 2675
AsuII TTCGAA 1 cut(s) 384
AsuNHI GCTAGC 1 cut(s) 2090
AvaI CYCGRG 5 cut(s) 35, 1054, 2121, 2559, 2685
AvaII GGWCC 2 cut(s) 19, 1997
BaeGI GKGCMC 3 cut(s) 910, 1240, 2434
BaeI ACNNNNGTAYC 4 cut(s) 172, 205, 411, 444
BamHI GGATCC 1 cut(s) 1087
BanII GRGCYC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 5 cut(s) 42, 1455, 2298, 2434, 2655
BbvI GCAGC 7 cut(s) 883, 1126, 1195, 1339, 2557, 2668, 2807
BccI CCATC 5 cut(s) 1247, 1625, 2047, 2258, 2728
BceAI ACGGC 1 cut(s) 221
BciT130I CCWGG 5 cut(s) 366, 783, 920, 1616, 2484
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 2 cut(s) 1366, 2494
BcoDI GTCTC 4 cut(s) 66, 260, 2177, 2723
BfaI CTAG 8 cut(s) 47, 615, 1061, 1241, 1830, 1851, 2091, 2827
BfmI CTRYAG 4 cut(s) 1773, 2208, 2598, 2619
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 9 cut(s) 897, 1115, 1145, 1184, 1353, 2452, 2571, 2682, 2796
BlsI GCNGC 9 cut(s) 898, 1116, 1146, 1185, 1354, 2453, 2572, 2683, 2797
BmcAI AGTACT 1 cut(s) 507
Bme1390I CCNGG 5 cut(s) 366, 783, 920, 1616, 2484
Bme18I GGWCC 2 cut(s) 19, 1997
BmeT110I CYCGRG 5 cut(s) 35, 1054, 2121, 2559, 2685
BmgT120I GGNCC 3 cut(s) 19, 1997, 2283
BmiI GGNNCC 6 cut(s) 736, 1089, 1293, 1982, 1999, 2813
BmrFI CCNGG 5 cut(s) 366, 783, 920, 1616, 2484
BmrI ACTGGG 2 cut(s) 419, 1939
BmsI GCATC 9 cut(s) 40, 402, 628, 925, 1126, 1140, 2198, 2605, 2782
BmtI GCTAGC 1 cut(s) 2094
BmuI ACTGGG 2 cut(s) 419, 1939
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 2 cut(s) 2573, 2605
BpmI CTGGAG 3 cut(s) 2600, 2723, 2798
Bpu10I CCTNAGC 1 cut(s) 1923
Bpu14I TTCGAA 1 cut(s) 384
BpuEI CTTGAG 8 cut(s) 26, 48, 319, 332, 1181, 1319, 1394, 2201
BsaAI YACGTR 1 cut(s) 2150
BsaBI GATNNNNATC 2 cut(s) 1494, 1677
BsaJI CCNNGG 4 cut(s) 373, 1275, 2245, 2483
BsaWI WCCGGW 1 cut(s) 1870
Bsc4I CCNNNNNNNGG 3 cut(s) 1281, 1877, 2006
Bse1I ACTGG 8 cut(s) 237, 294, 425, 746, 1856, 1934, 2583, 2665
Bse3DI GCAATG 3 cut(s) 629, 797, 1335
Bse8I GATNNNNATC 2 cut(s) 1494, 1677
BseBI CCWGG 5 cut(s) 366, 783, 920, 1616, 2484
BseDI CCNNGG 4 cut(s) 373, 1275, 2245, 2483
BseGI GGATG 8 cut(s) 10, 1042, 1636, 1804, 1879, 1906, 2782, 2797
BseJI GATNNNNATC 2 cut(s) 1494, 1677
BseLI CCNNNNNNNGG 3 cut(s) 1281, 1877, 2006
BseMI GCAATG 3 cut(s) 629, 797, 1335
BseMII CTCAG 3 cut(s) 1749, 1937, 2100
BseNI ACTGG 8 cut(s) 237, 294, 425, 746, 1856, 1934, 2583, 2665
BseRI GAGGAG 2 cut(s) 1255, 2720
BseSI GKGCMC 3 cut(s) 910, 1240, 2434
BseXI GCAGC 7 cut(s) 883, 1126, 1195, 1339, 2557, 2668, 2807
BseYI CCCAGC 2 cut(s) 1411, 1778
BsgI GTGCAG 3 cut(s) 992, 1077, 1407
Bsh1236I CGCG 1 cut(s) 277
Bsh1285I CGRYCG 1 cut(s) 721
BshFI GGCC 5 cut(s) 231, 1826, 1855, 2244, 2285
BsiEI CGRYCG 1 cut(s) 721
BsiHKAI GWGCWC 5 cut(s) 42, 1455, 2298, 2434, 2655
BsiHKCI CYCGRG 5 cut(s) 35, 1054, 2121, 2559, 2685
BsiSI CCGG 1 cut(s) 1871
BslFI GGGAC 2 cut(s) 146, 2263
BslI CCNNNNNNNGG 3 cut(s) 1281, 1877, 2006
BsmAI GTCTC 4 cut(s) 66, 260, 2177, 2723
BsmBI CGTCTC 1 cut(s) 2723
BsmFI GGGAC 2 cut(s) 146, 2263
BsmI GAATGC 2 cut(s) 590, 852
BsnI GGCC 5 cut(s) 231, 1826, 1855, 2244, 2285
BsoBI CYCGRG 5 cut(s) 35, 1054, 2121, 2559, 2685
Bsp119I TTCGAA 1 cut(s) 384
Bsp1286I GDGCHC 7 cut(s) 42, 910, 1240, 1455, 2298, 2434, 2655
Bsp1407I TGTACA 1 cut(s) 2469
BspACI CCGC 3 cut(s) 219, 1144, 2452
BspANI GGCC 5 cut(s) 231, 1826, 1855, 2244, 2285
BspCNI CTCAG 3 cut(s) 1750, 1936, 2099
BspFNI CGCG 1 cut(s) 277
BspHI TCATGA 2 cut(s) 2344, 2365
BspLI GGNNCC 6 cut(s) 736, 1089, 1293, 1982, 1999, 2813
BspOI GCTAGC 1 cut(s) 2094
BspPI GGATC 5 cut(s) 229, 720, 1082, 1095, 1680
BspQI GCTCTTC 2 cut(s) 1428, 1566
BspT104I TTCGAA 1 cut(s) 384
BsrBI CCGCTC 1 cut(s) 1144
BsrDI GCAATG 3 cut(s) 629, 797, 1335
BsrGI TGTACA 1 cut(s) 2469
BsrI ACTGG 8 cut(s) 237, 294, 425, 746, 1856, 1934, 2583, 2665
BssECI CCNNGG 4 cut(s) 373, 1275, 2245, 2483
BssT1I CCWWGG 2 cut(s) 373, 1275
Bst2UI CCWGG 5 cut(s) 366, 783, 920, 1616, 2484
Bst4CI ACNGT 5 cut(s) 188, 472, 1260, 1419, 2602
Bst6I CTCTTC 4 cut(s) 1428, 1566, 1689, 2619
BstAPI GCANNNNNTGC 6 cut(s) 585, 785, 1421, 2176, 2525, 2752
BstAUI TGTACA 1 cut(s) 2469
BstBAI YACGTR 1 cut(s) 2150
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 4 cut(s) 29, 2092, 2134, 2748
BstDEI CTNAG 5 cut(s) 1504, 1758, 1808, 1923, 2086
BstF5I GGATG 8 cut(s) 10, 1042, 1636, 1804, 1879, 1906, 2782, 2797
BstFNI CGCG 1 cut(s) 277
BstHHI GCGC 2 cut(s) 439, 2692
BstMAI GTCTC 4 cut(s) 66, 260, 2177, 2723
BstMCI CGRYCG 1 cut(s) 721
BstNI CCWGG 5 cut(s) 366, 783, 920, 1616, 2484
BstNSI RCATGY 2 cut(s) 1015, 1550
BstSCI CCNGG 5 cut(s) 364, 781, 918, 1614, 2482
BstSFI CTRYAG 4 cut(s) 1773, 2208, 2598, 2619
BstSLI GKGCMC 3 cut(s) 910, 1240, 2434
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 7 cut(s) 883, 1126, 1195, 1339, 2557, 2668, 2807
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 3 cut(s) 221, 712, 1087
BstXI CCANNNNNNTGG 1 cut(s) 2150
BstYI RGATCY 3 cut(s) 221, 712, 1087
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 5 cut(s) 231, 1826, 1855, 2244, 2285
BtgZI GCGATG 1 cut(s) 154
BtsCI GGATG 8 cut(s) 10, 1042, 1636, 1804, 1879, 1906, 2782, 2797
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 7 cut(s) 195, 244, 432, 1268, 1415, 1424, 2658
Cac8I GCNNGC 4 cut(s) 29, 2092, 2134, 2748
CaiI CAGNNNCTG 2 cut(s) 1120, 2752
CciI TCATGA 2 cut(s) 2344, 2365
CfoI GCGC 2 cut(s) 439, 2692
Cfr13I GGNCC 3 cut(s) 19, 1997, 2283
CsiI ACCWGGT 1 cut(s) 781
Csp6I GTAC 7 cut(s) 189, 506, 981, 1070, 1387, 1533, 2470
CspCI CAANNNNNGTGG 2 cut(s) 1990, 2025
CviAII CATG 8 cut(s) 149, 635, 1012, 1390, 1547, 2345, 2366, 2423
CviQI GTAC 7 cut(s) 189, 506, 981, 1070, 1387, 1533, 2470
DdeI CTNAG 5 cut(s) 1504, 1758, 1808, 1923, 2086
DraI TTTAAA 2 cut(s) 1518, 2742
DraIII CACNNNGTG 1 cut(s) 785
Eam1104I CTCTTC 4 cut(s) 1428, 1566, 1689, 2619
EarI CTCTTC 4 cut(s) 1428, 1566, 1689, 2619
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 2 cut(s) 373, 1275
Eco24I GRGCYC 1 cut(s) 42
Eco32I GATATC 1 cut(s) 1395
Eco47I GGWCC 2 cut(s) 19, 1997
Eco53kI GAGCTC 1 cut(s) 40
Eco88I CYCGRG 5 cut(s) 35, 1054, 2121, 2559, 2685
EcoICRI GAGCTC 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 1997
EcoRII CCWGG 5 cut(s) 364, 781, 918, 1614, 2482
EcoRV GATATC 1 cut(s) 1395
EcoT14I CCWWGG 2 cut(s) 373, 1275
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 2 cut(s) 373, 1275
Esp3I CGTCTC 1 cut(s) 2723
FaeI CATG 8 cut(s) 152, 638, 1015, 1393, 1550, 2348, 2369, 2426
FalI AAGNNNNNCTT 2 cut(s) 2163, 2195
FaqI GGGAC 2 cut(s) 146, 2263
FatI CATG 8 cut(s) 148, 634, 1011, 1389, 1546, 2344, 2365, 2422
FbaI TGATCA 2 cut(s) 1366, 2494
FblI GTMKAC 1 cut(s) 1903
Fnu4HI GCNGC 9 cut(s) 897, 1115, 1145, 1184, 1353, 2452, 2571, 2682, 2796
FokI GGATG 8 cut(s) 17, 1049, 1643, 1811, 1886, 1893, 2789, 2804
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 9 cut(s) 897, 1115, 1145, 1184, 1353, 2452, 2571, 2682, 2796
FspBI CTAG 8 cut(s) 47, 615, 1061, 1241, 1830, 1851, 2091, 2827
GlaI GCGC 2 cut(s) 438, 2691
GluI GCNGC 9 cut(s) 897, 1115, 1145, 1184, 1353, 2452, 2571, 2682, 2796
GsaI CCCAGC 2 cut(s) 1415, 1782
GsuI CTGGAG 3 cut(s) 2600, 2723, 2798
HaeIII GGCC 5 cut(s) 231, 1826, 1855, 2244, 2285
HapII CCGG 1 cut(s) 1871
HhaI GCGC 2 cut(s) 439, 2692
Hin1II CATG 8 cut(s) 152, 638, 1015, 1393, 1550, 2348, 2369, 2426
Hin6I GCGC 2 cut(s) 437, 2690
HinP1I GCGC 2 cut(s) 437, 2690
HincII GTYRAC 4 cut(s) 666, 1360, 1726, 2460
HindII GTYRAC 4 cut(s) 666, 1360, 1726, 2460
HindIII AAGCTT 2 cut(s) 29, 1655
HinfI GANTC 8 cut(s) 101, 554, 890, 1442, 1557, 2084, 2268, 2441
HpaI GTTAAC 2 cut(s) 666, 1726
HpaII CCGG 1 cut(s) 1871
HphI GGTGA 5 cut(s) 164, 361, 1588, 1945, 2675
Hpy166II GTNNAC 8 cut(s) 666, 1360, 1535, 1726, 1904, 1958, 2432, 2460
Hpy188I TCNGA 8 cut(s) 1122, 1135, 1556, 1713, 1759, 1792, 2030, 2052
Hpy8I GTNNAC 8 cut(s) 666, 1360, 1535, 1726, 1904, 1958, 2432, 2460
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 5 cut(s) 599, 1561, 1871, 1889, 2767
HpyCH4III ACNGT 5 cut(s) 188, 472, 1260, 1419, 2602
HpyCH4IV ACGT 1 cut(s) 2149
HpyF3I CTNAG 5 cut(s) 1504, 1758, 1808, 1923, 2086
HpySE526I ACGT 1 cut(s) 2149
Hsp92II CATG 8 cut(s) 152, 638, 1015, 1393, 1550, 2348, 2369, 2426
HspAI GCGC 2 cut(s) 437, 2690
Ksp22I TGATCA 2 cut(s) 1366, 2494
KspAI GTTAAC 2 cut(s) 666, 1726
LguI GCTCTTC 2 cut(s) 1428, 1566
LmnI GCTCC 6 cut(s) 740, 1450, 2129, 2204, 2678, 2817
Lsp1109I GCAGC 7 cut(s) 883, 1126, 1195, 1339, 2557, 2668, 2807
LweI GCATC 9 cut(s) 40, 402, 628, 925, 1126, 1140, 2198, 2605, 2782
MabI ACCWGGT 1 cut(s) 781
MaeI CTAG 8 cut(s) 47, 615, 1061, 1241, 1830, 1851, 2091, 2827
MaeII ACGT 1 cut(s) 2149
MaeIII GTNAC 4 cut(s) 536, 2081, 2217, 2602
MbiI CCGCTC 1 cut(s) 1144
MflI RGATCY 3 cut(s) 221, 712, 1087
MhlI GDGCHC 7 cut(s) 42, 910, 1240, 1455, 2298, 2434, 2655
MluI ACGCGT 1 cut(s) 275
MlyI GAGTC 3 cut(s) 1451, 2078, 2435
MmeI TCCRAC 3 cut(s) 320, 1428, 2053
Mph1103I ATGCAT 1 cut(s) 643
MroXI GAANNNNTTC 2 cut(s) 588, 747
MslI CAYNNNNRTG 5 cut(s) 1911, 2343, 2370, 2390, 2427
MspI CCGG 1 cut(s) 1871
MspR9I CCNGG 5 cut(s) 366, 783, 920, 1616, 2484
Mva1269I GAATGC 2 cut(s) 590, 852
MvaI CCWGG 5 cut(s) 366, 783, 920, 1616, 2484
MvnI CGCG 1 cut(s) 277
NheI GCTAGC 1 cut(s) 2090
NlaIII CATG 8 cut(s) 152, 638, 1015, 1393, 1550, 2348, 2369, 2426
NlaIV GGNNCC 6 cut(s) 736, 1089, 1293, 1982, 1999, 2813
NmeAIII GCCGAG 1 cut(s) 2270
NmuCI GTSAC 4 cut(s) 536, 2081, 2217, 2602
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 2 cut(s) 1015, 1550
NspV TTCGAA 1 cut(s) 384
PaeR7I CTCGAG 2 cut(s) 1054, 2559
PagI TCATGA 2 cut(s) 2344, 2365
PciI ACATGT 2 cut(s) 1011, 1546
PciSI GCTCTTC 2 cut(s) 1428, 1566
PctI GAATGC 2 cut(s) 590, 852
PdmI GAANNNNTTC 2 cut(s) 588, 747
PfeI GAWTC 5 cut(s) 101, 554, 890, 1557, 2268
PfoI TCCNGGA 2 cut(s) 918, 1614
PkrI GCNGC 9 cut(s) 898, 1116, 1146, 1185, 1354, 2453, 2572, 2683, 2797
PleI GAGTC 3 cut(s) 1450, 2078, 2435
PpsI GAGTC 3 cut(s) 1450, 2078, 2435
Ppu21I YACGTR 1 cut(s) 2150
PpuMI RGGWCCY 1 cut(s) 1997
PscI ACATGT 2 cut(s) 1011, 1546
Psp124BI GAGCTC 1 cut(s) 42
Psp5II RGGWCCY 1 cut(s) 1997
Psp6I CCWGG 5 cut(s) 364, 781, 918, 1614, 2482
PspFI CCCAGC 2 cut(s) 1411, 1778
PspGI CCWGG 5 cut(s) 364, 781, 918, 1614, 2482
PspN4I GGNNCC 6 cut(s) 736, 1089, 1293, 1982, 1999, 2813
PspPI GGNCC 3 cut(s) 19, 1997, 2283
PspPPI RGGWCCY 1 cut(s) 1997
PspXI VCTCGAGB 1 cut(s) 1054
PsrI GAACNNNNNNTAC 2 cut(s) 289, 321
PstNI CAGNNNCTG 2 cut(s) 1120, 2752
PsuI RGATCY 3 cut(s) 221, 712, 1087
RsaI GTAC 7 cut(s) 190, 507, 982, 1071, 1388, 1534, 2471
RsaNI GTAC 7 cut(s) 189, 506, 981, 1070, 1387, 1533, 2470
RseI CAYNNNNRTG 5 cut(s) 1911, 2343, 2370, 2390, 2427
SacI GAGCTC 1 cut(s) 42
SapI GCTCTTC 2 cut(s) 1428, 1566
SatI GCNGC 9 cut(s) 897, 1115, 1145, 1184, 1353, 2452, 2571, 2682, 2796
Sau96I GGNCC 3 cut(s) 19, 1997, 2283
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 3 cut(s) 1451, 2078, 2435
ScrFI CCNGG 5 cut(s) 366, 783, 920, 1616, 2484
SduI GDGCHC 7 cut(s) 42, 910, 1240, 1455, 2298, 2434, 2655
SexAI ACCWGGT 1 cut(s) 781
SfaNI GCATC 9 cut(s) 40, 402, 628, 925, 1126, 1140, 2198, 2605, 2782
SfcI CTRYAG 4 cut(s) 1773, 2208, 2598, 2619
Sfr274I CTCGAG 2 cut(s) 1054, 2559
SfuI TTCGAA 1 cut(s) 384
SinI GGWCC 2 cut(s) 19, 1997
SlaI CTCGAG 2 cut(s) 1054, 2559
SmiMI CAYNNNNRTG 5 cut(s) 1911, 2343, 2370, 2390, 2427
SsiI CCGC 3 cut(s) 219, 1144, 2452
SspI AATATT 1 cut(s) 2047
SspMI CTAG 8 cut(s) 47, 615, 1061, 1241, 1830, 1851, 2091, 2827
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 5 cut(s) 364, 781, 918, 1614, 2482
StyI CCWWGG 2 cut(s) 373, 1275
TaaI ACNGT 5 cut(s) 188, 472, 1260, 1419, 2602
TaiI ACGT 1 cut(s) 2152
TaqI TCGA 8 cut(s) 115, 384, 751, 1055, 1140, 1494, 1624, 2560
TaqII GACCGA 1 cut(s) 707
TatI WGTACW 4 cut(s) 505, 980, 1386, 2469
TauI GCSGC 2 cut(s) 1147, 2454
TfiI GAWTC 5 cut(s) 101, 554, 890, 1557, 2268
TscAI CASTG 7 cut(s) 202, 244, 432, 1275, 1422, 1431, 2665
TseFI GTSAC 4 cut(s) 536, 2081, 2217, 2602
TseI GCWGC 7 cut(s) 896, 1114, 1183, 1352, 2570, 2681, 2795
Tsp45I GTSAC 4 cut(s) 536, 2081, 2217, 2602
TspGWI ACGGA 1 cut(s) 1600
TspRI CASTG 7 cut(s) 202, 244, 432, 1275, 1422, 1431, 2665
VneI GTGCAC 1 cut(s) 2430
VpaK11BI GGWCC 2 cut(s) 19, 1997
XapI RAATTY 7 cut(s) 521, 686, 946, 1128, 1465, 1640, 1936
XceI RCATGY 2 cut(s) 1015, 1550
XhoI CTCGAG 2 cut(s) 1054, 2559
XmiI GTMKAC 1 cut(s) 1903
XmnI GAANNNNTTC 2 cut(s) 588, 747
XspI CTAG 8 cut(s) 47, 615, 1061, 1241, 1830, 1851, 2091, 2827
ZrmI AGTACT 1 cut(s) 507
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.