MD14G1221900.v1.1

Component of the post-replicative DNA mismatch repair system (MMR)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006881
Physical Location & Seq
Forward (+)
30364729 .. 30369841
5113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1221900.v1.1.491

Sequence Viewer

Length: 2829 bp
ATGGATGCCAATTTTGAGGACCACAGCAAGCTTCCCGAGCTCAAGCTAGATGCAAAGCAATCTCAAGGGTTTCTCTCATTCTTCAAAACCCTCCCCAATGATTCAAGAGCTATTCGATTGTTTGATCGACGGGACTACTATACCGCGCATGGTGAAAATGCCACTTTCATTGCAAAGACCTACTACCGTACCACCACTGCTTTACGACAGTTGGGTAGCGGATCCAATGGCCTATCCAGTGTGAGTGTTAGTAAAAACATGTTTGAGACGATTGCACGTGATCTAATACTGGAAAGAACAGACCATACTCTTGAGATTTATGAGGGTAGTGGCTCAAGTTGGAGGCTGGTGAAAAGTGGAACCCCTGGAAACCTTTGCAGTTTCGAAGATGTTTTGTTTGCCAACAATGAGATGCAGGATACCCCAGTGGTTGTTGCGCTATTACCTAACTTTCAGGAAAATGGTTGCACAGTTGGGTTAGGATATGTTGATCTAACTAAACGAGTACTTGGATTAGCTGAATTTATTGATGATAGTCACTTTACAAATGTGGAATCAGCTTTAGTTGCACTTGGTTGCAAGGAATGCCTTCTGCCTCTGGAGAGTGGCAAAACTAGCGAAATTAGAACATTGCATGATGCATTGAGTAGATGTGGTGTGATGTTAACCGAGAGGAAGAAGACTGAATTTAAGATGAGGGATTTGGTTCAGGATCTCAGTCGGCTTGTCAAAGGCTCCATTGAACCAGTTAGAGATTTAGTGTCGGGGTTTGAGTTTGCACCTGGTGCATTGGGGGCATTGCTCTCTTATGCAGAATTACTTGCTGACGAGAGCAATTATGGAAATTATAGCATTCAAAGATATAATCTTGACAGCTGTATGAGGCTAGATTCTGCTGCTATGAGGGCACTTAATGTCCTGGAAAGCAAAACCGATGCAAACAAAAATTTCAGTTTGTTTGGTCTTATGAATAGGACTTGTACTGCTGGAATGGGTAAAAGATTACTGCACATGTGGCTCAAACAGCCTTTATTGGATGTAAATGAAATCAACTCAAGGTTAGATTTGGTACAAGCATTTGTGGAGGATCCTGCACTTCGCCATGATTTGAGGCAGCATCTGAAAAGAATTTCAGACATCGAGCGGCTAATGCACAATCTTGAGAAGAAAAGAGCTGGTTTGCAGCATATTGTAAAACTTTATCAGTCGTGTATAAGACTTCCCTACATTAAGAGTGCCCTAGAACGCTATGATGGACAGTTCTCCTCACTGACCAAGGAAAGGTATTGGGAACCCCTTGAGTTATGGACTGATGATAGCCACTTAAATAAGTTCATTTCTCTGGTGGAAGCTGCTGTTGACCTTGATCAACTTGAGAATGGAGAGTACATGATATCATCTAGTTATGACCCCACACTTTCTGCACTGAAGGAAGAGCAGGAGTCATTGGAGCACCAAATACACACTATGCATAAACAAACCGCCAATGATCTTGATCTTGCTGTAGATAAGGCTTTAAAATTAGATAAAGGCACACAATTTGGACATGTCTTTAGAATCACAAAGAAGGAAGAGCCAAAAGTAAGGAAAAAGCTCACAACACAGTTCATTGTTCTGGAAACCCGAAAGGATGGAGTGAAATTTACCAACACAAAGCTTAAAAAGTTAGGGGATCAGTATCAAACGATAGTTGAAGAGTATAAGAATTGTCAGAAAGAGCTTGTTAACCGAGTTGTTCAAACTGCAACAACTTTCTCTGAGGTGTTCTGGTCTGTAGCTGGGTTGCTCTCTGAATTGGATGTCTTACTTGGTTTTGCTGATTTGGCTTCTAGCTGTCCTACTCCTTACACTAGGCCAGTCATCATGCCACCGGATGAAGGGGATATAATATTAGAAGGGAGTAGACATCCCTGTGTGGAGGCTCAGGACTGGGTGAATTTTATACCCAACGATTGTAAACTTGTGAGAGGAAAGAGTTGGTTCCAAATCATCACAGGACCCAATATGGGTGGCAAATCAACATTTATCCGACAGGTTGGTGTGAATATTCTGATGGCACAAGTTGGTTGTTTCGTTCCTTGTGACGCAGCTAATATTTCTGTTCGTGATTGCATTTTTGCACGGGTGGGAGCTGGCGATTGCCAACTACGTGGAGTTTCTACCTTTATGCAAGAAATGCTTGAGACTGCATCTATACTGAAAGGAGCTACAGACAAGTCACTGATAATCATCGATGAGTTAGGCCGAGGGACTTCAACTTATGATGGATTCGGTTTAGCTTGGGCCATTTGTGAACACCTTGTTGAAGTGATTAAAGCACCTACCTTGTTTGCAACCCACTTCCATGAATTAACTGAATTAGCTCATGAAAATGGCGTTCAGGAAGCTAATATGAAGCAAATTGTTGGCGTAGCAAACTATCACGTTAGTGCGCATATTGACTCATCAAGCCGCAAGTTGACTATGCTGTACAAGGTTGAGCCAGGAGCTTGTGATCAAAGTTTCGGTATTCAGGTTGCAGAGTTTGCAAACTTTCCTGAAAGTGTTGTTTCCCTTGCTCGAGAAAAAGCTGCTGAATTGGAGGATTTCTCTGCTACTCCAGTCACGCCAAATGATGCTGGAGAAGAGGTTGGATTGAAGCGTAAGAGAGAGCCCGACTCTGATGATATGTCCAGAGGAGCTGCTCGGGCGCACAAGTTTCTGAAGGAGTTTTCGGATTTGCCTCTGGAGACAGTGGATTTAAAACAGGCATTGCAAAAGGTTGGCAAGATGAAGGATGAGTTGCAAAAGGATGCAGCAAATTGTCAATGGCTCCAGCAGTTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000217 GO:0000400 GO:0000403 GO:0000404 GO:0000406 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006290 GO:0006298 GO:0006301 GO:0006310 GO:0006311 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0008094 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010520 GO:0010564 GO:0010605 GO:0010639 GO:0010948 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0030983 GO:0031323 GO:0031324 GO:0032135 GO:0032137 GO:0032138 GO:0032300 GO:0032301 GO:0032302 GO:0032991 GO:0033043 GO:0033554 GO:0034641 GO:0035822 GO:0040020 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045128 GO:0045786 GO:0045835 GO:0045910 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051321 GO:0051445 GO:0051447 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0060255 GO:0060631 GO:0061982 GO:0065007 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090304 GO:0097159 GO:0110029 GO:1901360 GO:1901363 GO:1903046 GO:1990391 GO:2000241 GO:2000242
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
MutS_I MutS_II MutS_III MutS_IV MutS_V

Protein Analysis

943

Amino Acids

105.65

Weight (kDa)

5.66

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MutS_I PF01624 23 - 128 1.2e-16 MutS domain I
MutS_II PF05188 150 - 283 3.4e-14 MutS domain II
MutS_III PF05192 299 - 606 3.6e-38 MutS domain III
MutS_IV PF05190 469 - 565 3.1e-19 MutS family domain IV
MutS_V PF00488 662 - 861 2.6e-78 MutS domain V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2433
AccBSI CCGCTC 1 cut(s) 1144
AccI GTMKAC 1 cut(s) 1903
AccII CGCG 1 cut(s) 146
AciI CCGC 5 cut(s) 144, 219, 1144, 1482, 2452
AclWI GGATC 6 cut(s) 216, 229, 720, 1082, 1095, 1680
AcsI RAATTY 6 cut(s) 521, 686, 946, 1128, 1640, 1936
AcuI CTGAAG 2 cut(s) 1448, 2723
AcvI CACGTG 1 cut(s) 278
AdeI CACNNNGTG 1 cut(s) 785
AfaI GTAC 6 cut(s) 190, 507, 982, 1071, 1388, 2471
AfiI CCNNNNNNNGG 3 cut(s) 1281, 1877, 2006
AflIII ACRYGT 3 cut(s) 258, 1011, 1546
AgsI TTSAA 9 cut(s) 85, 105, 743, 857, 1694, 1739, 2256, 2306, 2638
AjnI CCWGG 4 cut(s) 364, 781, 918, 2482
AleI CACNNNNGTG 1 cut(s) 2427
Alw21I GWGCWC 2 cut(s) 42, 1455
Alw26I GTCTC 3 cut(s) 260, 2177, 2723
AlwI GGATC 6 cut(s) 216, 229, 720, 1082, 1095, 1680
AlwNI CAGNNNCTG 1 cut(s) 1120
Ama87I CYCGRG 3 cut(s) 35, 2559, 2685
AoxI GGCC 4 cut(s) 229, 1853, 2242, 2283
ApeKI GCWGC 8 cut(s) 896, 1114, 1183, 1352, 2087, 2570, 2681, 2795
ApoI RAATTY 6 cut(s) 521, 686, 946, 1128, 1640, 1936
Asp700I GAANNNNTTC 1 cut(s) 588
AspLEI GCGC 4 cut(s) 148, 439, 2434, 2692
AspS9I GGNCC 3 cut(s) 19, 1997, 2283
AsuHPI GGTGA 3 cut(s) 164, 361, 1945
AsuII TTCGAA 1 cut(s) 384
AvaI CYCGRG 3 cut(s) 35, 2559, 2685
AvaII GGWCC 2 cut(s) 19, 1997
BaeGI GKGCMC 2 cut(s) 910, 1240
BaeI ACNNNNGTAYC 4 cut(s) 172, 205, 411, 444
BamHI GGATCC 2 cut(s) 221, 1087
BanII GRGCYC 2 cut(s) 42, 2655
BbrPI CACGTG 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 2 cut(s) 42, 1455
BbvI GCAGC 8 cut(s) 883, 1126, 1195, 1339, 2099, 2557, 2668, 2807
BccI CCATC 4 cut(s) 1247, 1625, 2047, 2258
BciT130I CCWGG 4 cut(s) 366, 783, 920, 2484
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 2 cut(s) 1366, 2494
BcoDI GTCTC 3 cut(s) 260, 2177, 2723
BfaI CTAG 8 cut(s) 47, 615, 887, 1241, 1401, 1830, 1851, 2827
BfmI CTRYAG 3 cut(s) 1503, 1773, 2208
BfuI GTATCC 1 cut(s) 412
BmcAI AGTACT 1 cut(s) 507
Bme1390I CCNGG 4 cut(s) 366, 783, 920, 2484
Bme18I GGWCC 2 cut(s) 19, 1997
BmeT110I CYCGRG 3 cut(s) 35, 2559, 2685
BmgT120I GGNCC 3 cut(s) 19, 1997, 2283
BmiI GGNNCC 8 cut(s) 223, 361, 736, 1089, 1293, 1982, 1999, 2813
BmrFI CCNGG 4 cut(s) 366, 783, 920, 2484
BmrI ACTGGG 2 cut(s) 419, 1939
BmsI GCATC 8 cut(s) 40, 402, 628, 925, 1126, 2198, 2605, 2782
BmuI ACTGGG 2 cut(s) 419, 1939
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 4 cut(s) 2573, 2605, 2642, 2674
BpmI CTGGAG 5 cut(s) 620, 2583, 2640, 2747, 2798
Bpu10I CCTNAGC 1 cut(s) 1923
Bpu14I TTCGAA 1 cut(s) 384
BpuEI CTTGAG 9 cut(s) 26, 48, 319, 332, 1039, 1181, 1319, 1394, 2201
Bsa29I ATCGAT 1 cut(s) 2232
BsaAI YACGTR 2 cut(s) 278, 2150
BsaBI GATNNNNATC 3 cut(s) 1494, 1677, 2228
BsaJI CCNNGG 3 cut(s) 364, 1275, 2245
BsaWI WCCGGW 1 cut(s) 1870
BsaXI ACNNNNNCTCC 2 cut(s) 2613, 2643
Bsc4I CCNNNNNNNGG 3 cut(s) 1281, 1877, 2006
Bse1I ACTGG 7 cut(s) 237, 294, 425, 746, 1856, 1934, 2600
Bse3DI GCAATG 4 cut(s) 168, 629, 797, 2750
Bse8I GATNNNNATC 3 cut(s) 1494, 1677, 2228
BseBI CCWGG 4 cut(s) 366, 783, 920, 2484
BseCI ATCGAT 1 cut(s) 2232
BseDI CCNNGG 3 cut(s) 364, 1275, 2245
BseGI GGATG 8 cut(s) 10, 1042, 1636, 1804, 1879, 1906, 2782, 2797
BseJI GATNNNNATC 3 cut(s) 1494, 1677, 2228
BseLI CCNNNNNNNGG 3 cut(s) 1281, 1877, 2006
BseMI GCAATG 4 cut(s) 168, 629, 797, 2750
BseMII CTCAG 3 cut(s) 730, 1749, 1937
BseNI ACTGG 7 cut(s) 237, 294, 425, 746, 1856, 1934, 2600
BseRI GAGGAG 2 cut(s) 1255, 2691
BseSI GKGCMC 2 cut(s) 910, 1240
BseXI GCAGC 8 cut(s) 883, 1126, 1195, 1339, 2099, 2557, 2668, 2807
BseYI CCCAGC 1 cut(s) 1778
BsgI GTGCAG 3 cut(s) 992, 1077, 1407
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 4 cut(s) 231, 1855, 2244, 2285
BshVI ATCGAT 1 cut(s) 2232
BsiHKAI GWGCWC 2 cut(s) 42, 1455
BsiHKCI CYCGRG 3 cut(s) 35, 2559, 2685
BsiSI CCGG 1 cut(s) 1871
BslFI GGGAC 2 cut(s) 146, 2263
BslI CCNNNNNNNGG 3 cut(s) 1281, 1877, 2006
BsmAI GTCTC 3 cut(s) 260, 2177, 2723
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 2 cut(s) 146, 2263
BsmI GAATGC 2 cut(s) 590, 852
BsnI GGCC 4 cut(s) 231, 1855, 2244, 2285
BsoBI CYCGRG 3 cut(s) 35, 2559, 2685
Bsp119I TTCGAA 1 cut(s) 384
Bsp1286I GDGCHC 5 cut(s) 42, 910, 1240, 1455, 2655
Bsp1407I TGTACA 1 cut(s) 2469
BspACI CCGC 5 cut(s) 144, 219, 1144, 1482, 2452
BspANI GGCC 4 cut(s) 231, 1855, 2244, 2285
BspCNI CTCAG 3 cut(s) 729, 1750, 1936
BspDI ATCGAT 1 cut(s) 2232
BspFNI CGCG 1 cut(s) 146
BspHI TCATGA 1 cut(s) 2365
BspLI GGNNCC 8 cut(s) 223, 361, 736, 1089, 1293, 1982, 1999, 2813
BspPI GGATC 6 cut(s) 216, 229, 720, 1082, 1095, 1680
BspQI GCTCTTC 2 cut(s) 1428, 1566
BspT104I TTCGAA 1 cut(s) 384
BsrBI CCGCTC 1 cut(s) 1144
BsrDI GCAATG 4 cut(s) 168, 629, 797, 2750
BsrGI TGTACA 1 cut(s) 2469
BsrI ACTGG 7 cut(s) 237, 294, 425, 746, 1856, 1934, 2600
BssECI CCNNGG 3 cut(s) 364, 1275, 2245
BssT1I CCWWGG 1 cut(s) 1275
Bst2UI CCWGG 4 cut(s) 366, 783, 920, 2484
Bst4CI ACNGT 6 cut(s) 188, 210, 472, 1260, 1605, 2734
Bst6I CTCTTC 4 cut(s) 1428, 1566, 1689, 2619
BstAPI GCANNNNNTGC 4 cut(s) 585, 785, 2176, 2525
BstAUI TGTACA 1 cut(s) 2469
BstBAI YACGTR 2 cut(s) 278, 2150
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 2 cut(s) 29, 2134
BstDEI CTNAG 3 cut(s) 716, 1758, 1923
BstF5I GGATG 8 cut(s) 10, 1042, 1636, 1804, 1879, 1906, 2782, 2797
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 4 cut(s) 148, 439, 2434, 2692
BstMAI GTCTC 3 cut(s) 260, 2177, 2723
BstNI CCWGG 4 cut(s) 366, 783, 920, 2484
BstNSI RCATGY 3 cut(s) 262, 1015, 1550
BstSCI CCNGG 4 cut(s) 364, 781, 918, 2482
BstSFI CTRYAG 3 cut(s) 1503, 1773, 2208
BstSLI GKGCMC 2 cut(s) 910, 1240
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 8 cut(s) 883, 1126, 1195, 1339, 2099, 2557, 2668, 2807
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 3 cut(s) 221, 712, 1087
BstXI CCANNNNNNTGG 1 cut(s) 2150
BstYI RGATCY 3 cut(s) 221, 712, 1087
Bsu15I ATCGAT 1 cut(s) 2232
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 4 cut(s) 231, 1855, 2244, 2285
BsuTUI ATCGAT 1 cut(s) 2232
BtsCI GGATG 8 cut(s) 10, 1042, 1636, 1804, 1879, 1906, 2782, 2797
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 7 cut(s) 195, 244, 432, 1268, 1424, 2219, 2739
Cac8I GCNNGC 2 cut(s) 29, 2134
CaiI CAGNNNCTG 1 cut(s) 1120
CciI TCATGA 1 cut(s) 2365
CfoI GCGC 4 cut(s) 148, 439, 2434, 2692
Cfr13I GGNCC 3 cut(s) 19, 1997, 2283
ClaI ATCGAT 1 cut(s) 2232
CseI GACGC 1 cut(s) 2093
CsiI ACCWGGT 1 cut(s) 781
Csp6I GTAC 6 cut(s) 189, 506, 981, 1070, 1387, 2470
CspCI CAANNNNNGTGG 4 cut(s) 151, 186, 1990, 2025
CviQI GTAC 6 cut(s) 189, 506, 981, 1070, 1387, 2470
DdeI CTNAG 3 cut(s) 716, 1758, 1923
DraI TTTAAA 2 cut(s) 1518, 2742
DraIII CACNNNGTG 1 cut(s) 785
Eam1104I CTCTTC 4 cut(s) 1428, 1566, 1689, 2619
EarI CTCTTC 4 cut(s) 1428, 1566, 1689, 2619
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 1275
Eco24I GRGCYC 2 cut(s) 42, 2655
Eco32I GATATC 1 cut(s) 1395
Eco47I GGWCC 2 cut(s) 19, 1997
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 2 cut(s) 1448, 2723
Eco72I CACGTG 1 cut(s) 278
Eco88I CYCGRG 3 cut(s) 35, 2559, 2685
EcoICRI GAGCTC 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 1997
EcoRII CCWGG 4 cut(s) 364, 781, 918, 2482
EcoRV GATATC 1 cut(s) 1395
EcoT14I CCWWGG 1 cut(s) 1275
EcoT22I ATGCAT 2 cut(s) 643, 1473
EcoT38I GRGCYC 2 cut(s) 42, 2655
ErhI CCWWGG 1 cut(s) 1275
Esp3I CGTCTC 1 cut(s) 260
FalI AAGNNNNNCTT 2 cut(s) 2163, 2195
FaqI GGGAC 2 cut(s) 146, 2263
FbaI TGATCA 2 cut(s) 1366, 2494
FblI GTMKAC 1 cut(s) 1903
FokI GGATG 8 cut(s) 17, 1049, 1643, 1811, 1886, 1893, 2789, 2804
FriOI GRGCYC 2 cut(s) 42, 2655
FspAI RTGCGCAY 1 cut(s) 2433
FspBI CTAG 8 cut(s) 47, 615, 887, 1241, 1401, 1830, 1851, 2827
FspI TGCGCA 1 cut(s) 2433
GlaI GCGC 4 cut(s) 147, 438, 2433, 2691
GsaI CCCAGC 1 cut(s) 1782
GsuI CTGGAG 5 cut(s) 620, 2583, 2640, 2747, 2798
HaeIII GGCC 4 cut(s) 231, 1855, 2244, 2285
HapII CCGG 1 cut(s) 1871
HgaI GACGC 1 cut(s) 2093
HhaI GCGC 4 cut(s) 148, 439, 2434, 2692
Hin6I GCGC 4 cut(s) 146, 437, 2432, 2690
HinP1I GCGC 4 cut(s) 146, 437, 2432, 2690
HincII GTYRAC 4 cut(s) 666, 1360, 1726, 2460
HindII GTYRAC 4 cut(s) 666, 1360, 1726, 2460
HindIII AAGCTT 2 cut(s) 29, 1655
HinfI GANTC 8 cut(s) 101, 554, 890, 1442, 1557, 2268, 2441, 2657
HpaI GTTAAC 2 cut(s) 666, 1726
HpaII CCGG 1 cut(s) 1871
HphI GGTGA 3 cut(s) 164, 361, 1945
Hpy166II GTNNAC 7 cut(s) 666, 1360, 1726, 1904, 1958, 2294, 2460
Hpy8I GTNNAC 7 cut(s) 666, 1360, 1726, 1904, 1958, 2294, 2460
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 7 cut(s) 599, 1423, 1561, 1871, 1889, 2698, 2767
HpyCH4III ACNGT 6 cut(s) 188, 210, 472, 1260, 1605, 2734
HpyCH4IV ACGT 3 cut(s) 277, 2149, 2424
HpyF3I CTNAG 3 cut(s) 716, 1758, 1923
HpySE526I ACGT 3 cut(s) 277, 2149, 2424
HspAI GCGC 4 cut(s) 146, 437, 2432, 2690
Ksp22I TGATCA 2 cut(s) 1366, 2494
KspAI GTTAAC 2 cut(s) 666, 1726
LguI GCTCTTC 2 cut(s) 1428, 1566
LmnI GCTCC 7 cut(s) 740, 1450, 2129, 2204, 2486, 2678, 2817
Lsp1109I GCAGC 8 cut(s) 883, 1126, 1195, 1339, 2099, 2557, 2668, 2807
LweI GCATC 8 cut(s) 40, 402, 628, 925, 1126, 2198, 2605, 2782
MabI ACCWGGT 1 cut(s) 781
MaeI CTAG 8 cut(s) 47, 615, 887, 1241, 1401, 1830, 1851, 2827
MaeII ACGT 3 cut(s) 277, 2149, 2424
MaeIII GTNAC 4 cut(s) 536, 2081, 2217, 2602
MbiI CCGCTC 1 cut(s) 1144
MboII GAAGA 9 cut(s) 73, 398, 688, 691, 1177, 1445, 1583, 1706, 2636
MflI RGATCY 3 cut(s) 221, 712, 1087
MhlI GDGCHC 5 cut(s) 42, 910, 1240, 1455, 2655
MlyI GAGTC 3 cut(s) 1451, 2435, 2651
MmeI TCCRAC 3 cut(s) 320, 2053, 2611
Mph1103I ATGCAT 2 cut(s) 643, 1473
MroXI GAANNNNTTC 1 cut(s) 588
MslI CAYNNNNRTG 4 cut(s) 1911, 2343, 2370, 2427
MspA1I CMGCKG 1 cut(s) 876
MspI CCGG 1 cut(s) 1871
MspR9I CCNGG 4 cut(s) 366, 783, 920, 2484
Mva1269I GAATGC 2 cut(s) 590, 852
MvaI CCWGG 4 cut(s) 366, 783, 920, 2484
MvnI CGCG 1 cut(s) 146
NlaIV GGNNCC 8 cut(s) 223, 361, 736, 1089, 1293, 1982, 1999, 2813
NmeAIII GCCGAG 1 cut(s) 2270
NmuCI GTSAC 4 cut(s) 536, 2081, 2217, 2602
NsbI TGCGCA 1 cut(s) 2433
NsiI ATGCAT 2 cut(s) 643, 1473
NspI RCATGY 3 cut(s) 262, 1015, 1550
NspV TTCGAA 1 cut(s) 384
OliI CACNNNNGTG 1 cut(s) 2427
PaeR7I CTCGAG 1 cut(s) 2559
PagI TCATGA 1 cut(s) 2365
PciI ACATGT 3 cut(s) 258, 1011, 1546
PciSI GCTCTTC 2 cut(s) 1428, 1566
PctI GAATGC 2 cut(s) 590, 852
PdmI GAANNNNTTC 1 cut(s) 588
PfeI GAWTC 5 cut(s) 101, 554, 890, 1557, 2268
PfoI TCCNGGA 1 cut(s) 918
PleI GAGTC 3 cut(s) 1450, 2435, 2651
PmaCI CACGTG 1 cut(s) 278
PmlI CACGTG 1 cut(s) 278
PpsI GAGTC 3 cut(s) 1450, 2435, 2651
Ppu21I YACGTR 2 cut(s) 278, 2150
PpuMI RGGWCCY 1 cut(s) 1997
PscI ACATGT 3 cut(s) 258, 1011, 1546
Psp124BI GAGCTC 1 cut(s) 42
Psp5II RGGWCCY 1 cut(s) 1997
Psp6I CCWGG 4 cut(s) 364, 781, 918, 2482
PspCI CACGTG 1 cut(s) 278
PspFI CCCAGC 1 cut(s) 1778
PspGI CCWGG 4 cut(s) 364, 781, 918, 2482
PspN4I GGNNCC 8 cut(s) 223, 361, 736, 1089, 1293, 1982, 1999, 2813
PspPI GGNCC 3 cut(s) 19, 1997, 2283
PspPPI RGGWCCY 1 cut(s) 1997
PsrI GAACNNNNNNTAC 2 cut(s) 289, 321
PstNI CAGNNNCTG 1 cut(s) 1120
PsuI RGATCY 3 cut(s) 221, 712, 1087
PvuII CAGCTG 1 cut(s) 876
RsaI GTAC 6 cut(s) 190, 507, 982, 1071, 1388, 2471
RsaNI GTAC 6 cut(s) 189, 506, 981, 1070, 1387, 2470
RseI CAYNNNNRTG 4 cut(s) 1911, 2343, 2370, 2427
SacI GAGCTC 1 cut(s) 42
SapI GCTCTTC 2 cut(s) 1428, 1566
Sau96I GGNCC 3 cut(s) 19, 1997, 2283
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 3 cut(s) 1451, 2435, 2651
ScrFI CCNGG 4 cut(s) 366, 783, 920, 2484
SduI GDGCHC 5 cut(s) 42, 910, 1240, 1455, 2655
SexAI ACCWGGT 1 cut(s) 781
SfaNI GCATC 8 cut(s) 40, 402, 628, 925, 1126, 2198, 2605, 2782
SfcI CTRYAG 3 cut(s) 1503, 1773, 2208
Sfr274I CTCGAG 1 cut(s) 2559
SfuI TTCGAA 1 cut(s) 384
SinI GGWCC 2 cut(s) 19, 1997
SlaI CTCGAG 1 cut(s) 2559
SmiMI CAYNNNNRTG 4 cut(s) 1911, 2343, 2370, 2427
SsiI CCGC 5 cut(s) 144, 219, 1144, 1482, 2452
SspI AATATT 3 cut(s) 1890, 2047, 2095
SspMI CTAG 8 cut(s) 47, 615, 887, 1241, 1401, 1830, 1851, 2827
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 4 cut(s) 364, 781, 918, 2482
StyI CCWWGG 1 cut(s) 1275
TaaI ACNGT 6 cut(s) 188, 210, 472, 1260, 1605, 2734
TaiI ACGT 3 cut(s) 280, 2152, 2427
TaqI TCGA 6 cut(s) 115, 127, 384, 1140, 2232, 2560
TatI WGTACW 4 cut(s) 505, 980, 1386, 2469
TauI GCSGC 2 cut(s) 1147, 2454
TfiI GAWTC 5 cut(s) 101, 554, 890, 1557, 2268
TscAI CASTG 7 cut(s) 202, 244, 432, 1275, 1431, 2226, 2739
TseFI GTSAC 4 cut(s) 536, 2081, 2217, 2602
TseI GCWGC 8 cut(s) 896, 1114, 1183, 1352, 2087, 2570, 2681, 2795
Tsp45I GTSAC 4 cut(s) 536, 2081, 2217, 2602
TspRI CASTG 7 cut(s) 202, 244, 432, 1275, 1431, 2226, 2739
VpaK11BI GGWCC 2 cut(s) 19, 1997
XapI RAATTY 6 cut(s) 521, 686, 946, 1128, 1640, 1936
XceI RCATGY 3 cut(s) 262, 1015, 1550
XhoI CTCGAG 1 cut(s) 2559
XmiI GTMKAC 1 cut(s) 1903
XmnI GAANNNNTTC 1 cut(s) 588
XspI CTAG 8 cut(s) 47, 615, 887, 1241, 1401, 1830, 1851, 2827
ZrmI AGTACT 1 cut(s) 507
Zsp2I ATGCAT 2 cut(s) 643, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.