Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009432

Physical Location & Seq

Genomic Coordinates Reverse (-)

34886303 .. 34890943

Gene Structure 4641 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1216600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGGCCTTCACAACCGCGAAAATTTCCGAAATGCCACTGAGGAACTCACTCCCACTCACCTCTCACTCGCCCTCCTCTTTCAGCCTGGCCCCAAAACCGTCATTTCGCATCGTCAAAACATTCTCCTCCTCCGTCAGAACCACCGAGCACGGCGGAAATCCGAACGCTAAACCCCCGCAGCACAAATCCAGGCCCGCCTCGCCCGCTCCTTGGCTCAACAAGTGGCCCAATCGCAGCTCTCCCGCCGAATTACCCCGCCAGAAAGTCAACGAGAAGGTCAACGAGTCGCAGGGACGAGAGCAAGATGGCAAAGCGGATTCGACTCGGTACTTTGACAAGAATAAAGGCCAGAGCGCGATTGAGAGAATCGTGTTCCGGTTGCGGAACTTAAGGTTAGGGTCGGACGATGAGGAGGAAGACGATGGAATCGGATTGGACGGTCAGGATTCAGTGCCGGCGGCAAGCGGAGAGGAGAAGCTCGGGGATTTGTTGCAGCGGGAGTGGGTCCGGCCGGATTATGTATTGGAGGAGGAGAAGAGCGCTGATGAGGTGGCATTGCCGTGGGAGAAAGAGGAGGAGGAGGAGGTGAATGACAAGGAGGAGGTCAAGGGGTTGAGGAAGAGGAGAGGGAAAGCTCCGTCCTTGGCGGAATTGACTATTGAGGACGAGGAGCTGAAGCGGCTGAGGAGAATGGGGATGGTGCTTAGGGAAAGGATCAGTGTGCCCAAGGCCGGGATCACCCAGGCGGTGCTAGAGAAGATTCACGATACATGGAGGAAGACGGAGCTGGTGAGGCTCAAGTTCCATGAGGTGCTGGCATTGGACATGAAGACGGCTCACGAGATTGTTGAGCGTCGAACAGGAGGGTTAGTTTTATGGCGATCAGGAAGTGTCATGGTTGTGTACCGAGGGAGTAACTACAAAGGACCTTCTCAATCCCAAACTGTTGATGGGGAAAGGGGTGGTCTTTTTATCCCAGATGTTTCTTCAGCTGAAACATCGGTGATGAGAAGTGGCAATGATGCAACTTCAGGTCCAGATAAGACTGAGCAAGCATTGAAAATTCCAGAGCGTGTTCATAACATGACGGAGGAGGAAGCTGAGTTTCACAGCCTATTGGATGATTTAGGTCCACGTTTTGAAGAGTGGTGGGGTACAGGAGTACTTCCTGTTGATGCTGATTTGCTTCCCAAAACAATTCCTGGCTACAAAACACCTTTCAGACTTCTCCCTACCGGGATGCGATCACGGCTGACTAATGCAGAGATGACTACCTTACGGAAACTTGCCAAGTCGCTTCCTTGTCATTTTGCCCTTGGGAGAAATAGAAACCATCAAGGGTTGGCATTGGCTATTATTAAACTTTGGGAGAAAAGCTCTGTTGCTAAGATTGCTGTCAAACGAGGTATCCAGAATACAAACAACAAGTTGATGGCTGAAGAGATAAAGAATTTAACAGGTGGCGTTTTACTGCTCAGAAACAAATATTACATTGTCATTTACCGTGGAAAGGACTTTCTTCCAACAAGTGTGGCTGCTGCTCTGGCTGAAAGACAGGAACTAACGAAACAGGTTCAAGATGTTGAAGAGAAAATGCGGATTAAAGCAATAGATGCAGCTTCTTCAGGTGCAGTGGAAGGACAGGCACTTGCAGGGACTTTGGCTGAGTTTTATGAGGCTCAAGCTCGTTGGGGACGAGAAATATCTGCTGAAGAACGGGAAAAGATGATTGAAGAAGATTCAAGAGCTAAGAATGCTAAGCTTGTCAGACGAATCGAGCATAAACTAGGTGTTGCCCAAGCCAAAAAGCTAAGAGCAGAGAAACTCCTCTCCAAGATAGAATCATTAATGCTTCCTGCGGGTCCCGATTATGATCAGGAAACAATCACTGATGAGGAACGGGTTATGTTTCGCCGGGTTGGTTTGAGAATGAAAGCATACTTGCCTCTTGGCATACGTGGTGTCTTTGATGGTGTTGTCGAAAACATGCACTTGCATTGGAAGCACAGAGAACTAGTGAAATTAATAAGCAAGCAAAAGACTCTTGCTTTTGTTGAAGATACAGCTAGGTTATTGGAATATGAGAGTGGTGGTATACTTGTGGCAATAGAAAGAGTTCCAAAGGGATATGCTCTCATTTACTATCGGGGAAAGAATTATCGGCGACCCATTACCTTGAGGCCTAGGAACCTTCTAACTAAGGCTAAAGCATTAAAACGATCAGTGGCCATGCAACGCCATGAGGCTCTCAGTCAGCACATATCTGAACTAGAGAAGAACATAGAGCAAATGAAATCTCAAGTTGGTATTTCTGAAGATGATGTAGATGAGAGTTCCTGGAGCTCGAGAGATCCCGAGCAGATTCATCGTGCGTTGGAATTAGTCCAAAGCGAGGACGAAGATGAGGATGAAGATGAAGATTCATACGTGGATTCAGATGTTAGCGACAATGATGAAGATTCTGATTGGGAAAACGAAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRS1_YhbY

Protein Analysis

828

Amino Acids

93.63

Weight (kDa)

6.08

Isoelectric Point (pI)

56.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRS1_YhbY	PF01985	221 - 303	7.4e-20	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	418 - 502	2e-15	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	629 - 716	3.4e-14	CRS1 / YhbY (CRM) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009432)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18390
fragaria_vesca	FvH4_5g12070 FvH4_5g12070 FvH4_5g12070 FvH4_5g12070
malus_domestica	MD06G1216600.v1.1 MD14G1227100.v1.1
prunus_persica	Prupe.5G221800_v2.0.a1
pyrus_communis	pycom06g19300 pycom14g18850
rosa_chinensis	RchiOBHm_Chr7g0185501
rosa_laevigata	RLG00000004907
rosa_multiflora	Rmu_sc0008223.1_g000008 Rmu_sc0010190.1_g000020 Rmu_sc0042727.1_g000001
rosa_roxburghii	Rroxscaffold_3G00268880
rosa_rugosa	Rorug06G0469800
rosa_samantha	Rh7AG072900 Rh7BG074700 Rh7CG074800 Rh7DG074700
rosa_wichuraiana	Rw7G006220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	206
AccI	GTMKAC	1 cut(s)	2095
AccII	CGCG	2 cut(s)	17, 356
AclWI	GGATC	3 cut(s)	722, 743, 2345
AcoI	YGGCCR	2 cut(s)	509, 2226
AcsI	RAATTY	3 cut(s)	21, 1062, 1450
AcuI	CTGAAG	7 cut(s)	695, 972, 1014, 1458, 1608, 1731, 2334
AfaI	GTAC	4 cut(s)	329, 905, 1156, 1164
AfeI	AGCGCT	1 cut(s)	541
AfiI	CCNNNNNNNGG	5 cut(s)	210, 731, 732, 1510, 2392
AflII	CTTAAG	1 cut(s)	388
AgsI	TTSAA	7 cut(s)	1060, 1142, 1577, 1586, 1733, 1743, 2057
AhlI	ACTAGT	1 cut(s)	2014
AjnI	CCWGG	5 cut(s)	84, 188, 741, 1201, 2336
Alw21I	GWGCWC	2 cut(s)	150, 2345
AlwI	GGATC	3 cut(s)	722, 743, 2345
Ama87I	CYCGRG	3 cut(s)	479, 2344, 2354
Aor51HI	AGCGCT	1 cut(s)	541
AoxI	GGCC	9 cut(s)	3, 87, 191, 224, 346, 509, 729, 2180, 2226
ApeKI	GCWGC	6 cut(s)	178, 234, 493, 1535, 1538, 1616
ApoI	RAATTY	3 cut(s)	21, 1062, 1450
AseI	ATTAAT	2 cut(s)	1847, 2024
Asp700I	GAANNNNTTC	1 cut(s)	2115
AspA2I	CCTAGG	1 cut(s)	2183
AspLEI	GCGC	2 cut(s)	356, 542
AspS9I	GGNCC	8 cut(s)	88, 192, 225, 505, 926, 1034, 1130, 1862
AsuC2I	CCSGG	3 cut(s)	733, 1237, 1916
AsuHPI	GGTGA	5 cut(s)	49, 598, 730, 802, 1015
AvaI	CYCGRG	3 cut(s)	479, 2344, 2354
AvaII	GGWCC	5 cut(s)	505, 926, 1034, 1130, 1862
AvrII	CCTAGG	1 cut(s)	2183
BaeGI	GKGCMC	1 cut(s)	726
BaeI	ACNNNNGTAYC	2 cut(s)	1146, 1179
BalI	TGGCCA	1 cut(s)	2228
BanII	GRGCYC	1 cut(s)	2345
BauI	CACGAG	1 cut(s)	839
BbsI	GAAGAC	3 cut(s)	423, 785, 836
Bbv12I	GWGCWC	2 cut(s)	150, 2345
BbvCI	CCTCAGC	1 cut(s)	683
BbvI	GCAGC	6 cut(s)	190, 246, 505, 1522, 1525, 1628
BccI	CCATC	7 cut(s)	299, 416, 691, 944, 1341, 1426, 1964
BceAI	ACGGC	4 cut(s)	166, 544, 849, 1265
BcgI	CGANNNNNNTGC	2 cut(s)	2348, 2382
BciT130I	CCWGG	5 cut(s)	86, 190, 743, 1203, 2338
BciVI	GTATCC	1 cut(s)	1418
BclI	TGATCA	1 cut(s)	1873
BcnI	CCSGG	3 cut(s)	733, 1237, 1916
BcuI	ACTAGT	1 cut(s)	2014
BfaI	CTAG	6 cut(s)	752, 1787, 2015, 2067, 2184, 2270
BfoI	RGCGCY	1 cut(s)	543
BfrI	CTTAAG	1 cut(s)	388
BfuI	GTATCC	1 cut(s)	1418
BisI	GCNGC	8 cut(s)	179, 235, 459, 494, 680, 1536, 1539, 1617
BlnI	CCTAGG	1 cut(s)	2183
BlpI	GCTNAGC	1 cut(s)	1758
BlsI	GCNGC	8 cut(s)	180, 236, 460, 495, 681, 1537, 1540, 1618
BmcAI	AGTACT	1 cut(s)	1164
Bme1390I	CCNGG	8 cut(s)	86, 190, 733, 743, 1203, 1237, 1916, 2338
Bme18I	GGWCC	5 cut(s)	505, 926, 1034, 1130, 1862
BmeT110I	CYCGRG	3 cut(s)	479, 2344, 2354
BmgT120I	GGNCC	8 cut(s)	88, 192, 225, 505, 926, 1034, 1130, 1862
BmiI	GGNNCC	5 cut(s)	90, 506, 1863, 1864, 2189
BmrFI	CCNGG	8 cut(s)	86, 190, 733, 743, 1203, 1237, 1916, 2338
BmsI	GCATC	5 cut(s)	117, 1012, 1165, 1230, 1603
BpiI	GAAGAC	3 cut(s)	423, 785, 836
BplI	GAGNNNNNCTC	2 cut(s)	1361, 1393
BpmI	CTGGAG	1 cut(s)	2359
Bpu10I	CCTNAGC	2 cut(s)	683, 704
Bpu1102I	GCTNAGC	1 cut(s)	1758
BpuEI	CTTGAG	4 cut(s)	782, 1665, 2197, 2283
BpuMI	CCSGG	3 cut(s)	733, 1237, 1916
BsaAI	YACGTR	2 cut(s)	1958, 2428
BsaBI	GATNNNNATC	1 cut(s)	1872
BsaJI	CCNNGG	9 cut(s)	209, 560, 642, 726, 741, 907, 1315, 1504, 2183
BsaWI	WCCGGW	1 cut(s)	375
BsaXI	ACNNNNNCTCC	4 cut(s)	56, 86, 855, 885
Bsc4I	CCNNNNNNNGG	5 cut(s)	210, 731, 732, 1510, 2392
Bse118I	RCCGGY	1 cut(s)	454
Bse3DI	GCAATG	2 cut(s)	554, 1024
Bse8I	GATNNNNATC	1 cut(s)	1872
BseBI	CCWGG	5 cut(s)	86, 190, 743, 1203, 2338
BseDI	CCNNGG	9 cut(s)	209, 560, 642, 726, 741, 907, 1315, 1504, 2183
BseGI	GGATG	4 cut(s)	702, 1126, 1245, 2413
BseJI	GATNNNNATC	1 cut(s)	1872
BseLI	CCNNNNNNNGG	5 cut(s)	210, 731, 732, 1510, 2392
BseMI	GCAATG	2 cut(s)	554, 1024
BseMII	CTCAG	7 cut(s)	29, 674, 1038, 1092, 1489, 1656, 2263
BseSI	GKGCMC	1 cut(s)	726
BseX3I	CGGCCG	1 cut(s)	509
BseXI	GCAGC	6 cut(s)	190, 246, 505, 1522, 1525, 1628
BsgI	GTGCAG	1 cut(s)	1650
Bsh1236I	CGCG	2 cut(s)	17, 356
Bsh1285I	CGRYCG	1 cut(s)	512
BshFI	GGCC	9 cut(s)	5, 89, 193, 226, 348, 511, 731, 2182, 2228
BsiEI	CGRYCG	1 cut(s)	512
BsiHKAI	GWGCWC	2 cut(s)	150, 2345
BsiHKCI	CYCGRG	3 cut(s)	479, 2344, 2354
BsiSI	CCGG	7 cut(s)	376, 455, 508, 512, 732, 1236, 1915
BslFI	GGGAC	4 cut(s)	306, 1669, 1707, 1848
BslI	CCNNNNNNNGG	5 cut(s)	210, 731, 732, 1510, 2392
BsmFI	GGGAC	4 cut(s)	306, 1669, 1707, 1848
BsmI	GAATGC	1 cut(s)	1759
BsnI	GGCC	9 cut(s)	5, 89, 193, 226, 348, 511, 731, 2182, 2228
BsoBI	CYCGRG	3 cut(s)	479, 2344, 2354
Bsp1286I	GDGCHC	3 cut(s)	150, 726, 2345
Bsp143I	GATC	7 cut(s)	714, 735, 881, 1244, 1873, 2219, 2350
Bsp1720I	GCTNAGC	1 cut(s)	1758
BspANI	GGCC	9 cut(s)	5, 89, 193, 226, 348, 511, 731, 2182, 2228
BspCNI	CTCAG	7 cut(s)	30, 675, 1039, 1093, 1488, 1657, 2262
BspFNI	CGCG	2 cut(s)	17, 356
BspLI	GGNNCC	5 cut(s)	90, 506, 1863, 1864, 2189
BspPI	GGATC	3 cut(s)	722, 743, 2345
BspQI	GCTCTTC	1 cut(s)	530
BspTI	CTTAAG	1 cut(s)	388
BsrBI	CCGCTC	1 cut(s)	206
BsrDI	GCAATG	2 cut(s)	554, 1024
BsrFI	RCCGGY	1 cut(s)	454
BssAI	RCCGGY	1 cut(s)	454
BssECI	CCNNGG	9 cut(s)	209, 560, 642, 726, 741, 907, 1315, 1504, 2183
BssMI	GATC	7 cut(s)	714, 735, 881, 1244, 1873, 2219, 2350
BssNAI	GTATAC	1 cut(s)	2096
BssSI	CACGAG	1 cut(s)	839
BssT1I	CCWWGG	5 cut(s)	209, 642, 726, 1315, 2183
Bst1107I	GTATAC	1 cut(s)	2096
Bst2BI	CACGAG	1 cut(s)	839
Bst2UI	CCWGG	5 cut(s)	86, 190, 743, 1203, 2338
Bst4CI	ACNGT	4 cut(s)	99, 440, 946, 1505
Bst6I	CTCTTC	5 cut(s)	530, 614, 1137, 1434, 1581
BstAFI	CTTAAG	1 cut(s)	388
BstAPI	GCANNNNNTGC	1 cut(s)	1613
BstBAI	YACGTR	2 cut(s)	1958, 2428
BstC8I	GCNNGC	7 cut(s)	195, 204, 456, 463, 816, 1053, 2033
BstDSI	CCRYGG	2 cut(s)	560, 1504
BstF5I	GGATG	4 cut(s)	702, 1126, 1245, 2413
BstFNI	CGCG	2 cut(s)	17, 356
BstH2I	RGCGCY	1 cut(s)	543
BstHHI	GCGC	2 cut(s)	356, 542
BstKTI	GATC	7 cut(s)	717, 738, 884, 1247, 1876, 2222, 2353
BstMBI	GATC	7 cut(s)	714, 735, 881, 1244, 1873, 2219, 2350
BstMCI	CGRYCG	1 cut(s)	512
BstNI	CCWGG	5 cut(s)	86, 190, 743, 1203, 2338
BstNSI	RCATGY	1 cut(s)	1990
BstSCI	CCNGG	8 cut(s)	84, 188, 731, 741, 1201, 1235, 1914, 2336
BstSLI	GKGCMC	1 cut(s)	726
BstUI	CGCG	2 cut(s)	17, 356
BstV1I	GCAGC	6 cut(s)	190, 246, 505, 1522, 1525, 1628
BstV2I	GAAGAC	3 cut(s)	423, 785, 836
BstX2I	RGATCY	1 cut(s)	2350
BstYI	RGATCY	1 cut(s)	2350
BstZ17I	GTATAC	1 cut(s)	2096
BstZI	CGGCCG	1 cut(s)	509
BsuI	GTATCC	1 cut(s)	1418
BsuRI	GGCC	9 cut(s)	5, 89, 193, 226, 348, 511, 731, 2182, 2228
BtgI	CCRYGG	2 cut(s)	560, 1504
BtsCI	GGATG	4 cut(s)	702, 1126, 1245, 2413
BtsI	GCAGTG	1 cut(s)	1638
BtsIMutI	CAGTG	6 cut(s)	35, 456, 724, 1638, 1887, 2229
Cac8I	GCNNGC	7 cut(s)	195, 204, 456, 463, 816, 1053, 2033
CfoI	GCGC	2 cut(s)	356, 542
Cfr10I	RCCGGY	1 cut(s)	454
Cfr13I	GGNCC	8 cut(s)	88, 192, 225, 505, 926, 1034, 1130, 1862
CseI	GACGC	1 cut(s)	842
Csp6I	GTAC	4 cut(s)	328, 904, 1155, 1163
CspCI	CAANNNNNGTGG	2 cut(s)	1512, 1547
CviAII	CATG	8 cut(s)	771, 806, 826, 895, 1084, 1987, 2230, 2240
CviQI	GTAC	4 cut(s)	328, 904, 1155, 1163
DpnI	GATC	7 cut(s)	716, 737, 883, 1246, 1875, 2221, 2352
DpnII	GATC	7 cut(s)	714, 735, 881, 1244, 1873, 2219, 2350
EaeI	YGGCCR	2 cut(s)	509, 2226
EagI	CGGCCG	1 cut(s)	509
Eam1104I	CTCTTC	5 cut(s)	530, 614, 1137, 1434, 1581
EarI	CTCTTC	5 cut(s)	530, 614, 1137, 1434, 1581
EciI	GGCGGA	2 cut(s)	168, 662
Ecl136II	GAGCTC	1 cut(s)	2343
EclXI	CGGCCG	1 cut(s)	509
Eco130I	CCWWGG	5 cut(s)	209, 642, 726, 1315, 2183
Eco147I	AGGCCT	1 cut(s)	2182
Eco24I	GRGCYC	1 cut(s)	2345
Eco47I	GGWCC	5 cut(s)	505, 926, 1034, 1130, 1862
Eco47III	AGCGCT	1 cut(s)	541
Eco52I	CGGCCG	1 cut(s)	509
Eco53kI	GAGCTC	1 cut(s)	2343
Eco57I	CTGAAG	7 cut(s)	695, 972, 1014, 1458, 1608, 1731, 2334
Eco88I	CYCGRG	3 cut(s)	479, 2344, 2354
EcoICRI	GAGCTC	1 cut(s)	2343
EcoO109I	RGGNCCY	2 cut(s)	926, 1862
EcoRII	CCWGG	5 cut(s)	84, 188, 741, 1201, 2336
EcoT14I	CCWWGG	5 cut(s)	209, 642, 726, 1315, 2183
EcoT38I	GRGCYC	1 cut(s)	2345
ErhI	CCWWGG	5 cut(s)	209, 642, 726, 1315, 2183
FaeI	CATG	8 cut(s)	774, 809, 829, 898, 1087, 1990, 2233, 2243
FaqI	GGGAC	4 cut(s)	306, 1669, 1707, 1848
FatI	CATG	8 cut(s)	770, 805, 825, 894, 1083, 1986, 2229, 2239
FauI	CCCGC	7 cut(s)	183, 202, 211, 250, 263, 489, 1852
FbaI	TGATCA	1 cut(s)	1873
FblI	GTMKAC	1 cut(s)	2095
Fnu4HI	GCNGC	8 cut(s)	179, 235, 459, 494, 680, 1536, 1539, 1617
FokI	GGATG	4 cut(s)	709, 1133, 1252, 2420
FriOI	GRGCYC	1 cut(s)	2345
Fsp4HI	GCNGC	8 cut(s)	179, 235, 459, 494, 680, 1536, 1539, 1617
FspBI	CTAG	6 cut(s)	752, 1787, 2015, 2067, 2184, 2270
GlaI	GCGC	2 cut(s)	355, 541
GluI	GCNGC	8 cut(s)	179, 235, 459, 494, 680, 1536, 1539, 1617
GsuI	CTGGAG	1 cut(s)	2359
HaeII	RGCGCY	1 cut(s)	543
HaeIII	GGCC	9 cut(s)	5, 89, 193, 226, 348, 511, 731, 2182, 2228
HapII	CCGG	7 cut(s)	376, 455, 508, 512, 732, 1236, 1915
HgaI	GACGC	1 cut(s)	842
HhaI	GCGC	2 cut(s)	356, 542
Hin1II	CATG	8 cut(s)	774, 809, 829, 898, 1087, 1990, 2233, 2243
Hin6I	GCGC	2 cut(s)	354, 540
HinP1I	GCGC	2 cut(s)	354, 540
HincII	GTYRAC	2 cut(s)	268, 280
HindII	GTYRAC	2 cut(s)	268, 280
HindIII	AAGCTT	1 cut(s)	1760
HpaII	CCGG	7 cut(s)	376, 455, 508, 512, 732, 1236, 1915
HphI	GGTGA	5 cut(s)	49, 598, 730, 802, 1015
Hpy166II	GTNNAC	5 cut(s)	268, 280, 904, 1133, 2096
Hpy8I	GTNNAC	5 cut(s)	268, 280, 904, 1133, 2096
Hpy99I	CGWCG	1 cut(s)	858
HpyAV	CCTTC	5 cut(s)	16, 268, 939, 1631, 2201
HpyCH4III	ACNGT	4 cut(s)	99, 440, 946, 1505
HpyCH4IV	ACGT	3 cut(s)	1135, 1957, 2427
HpyCH4V	TGCA	9 cut(s)	493, 1025, 1262, 1616, 1631, 1652, 1990, 1996, 2233
HpySE526I	ACGT	3 cut(s)	1135, 1957, 2427
Hsp92II	CATG	8 cut(s)	774, 809, 829, 898, 1087, 1990, 2233, 2243
HspAI	GCGC	2 cut(s)	354, 540
KflI	GGGWCCC	1 cut(s)	1862
KroI	GCCGGC	1 cut(s)	454
KroNI	GCCGGC	1 cut(s)	456
Ksp22I	TGATCA	1 cut(s)	1873
Kzo9I	GATC	7 cut(s)	714, 735, 881, 1244, 1873, 2219, 2350
LguI	GCTCTTC	1 cut(s)	530
LmnI	GCTCC	5 cut(s)	211, 640, 670, 784, 2340
Lsp1109I	GCAGC	6 cut(s)	190, 246, 505, 1522, 1525, 1628
LweI	GCATC	5 cut(s)	117, 1012, 1165, 1230, 1603
MaeI	CTAG	6 cut(s)	752, 1787, 2015, 2067, 2184, 2270
MaeII	ACGT	3 cut(s)	1135, 1957, 2427
MaeIII	GTNAC	1 cut(s)	914
MalI	GATC	7 cut(s)	716, 737, 883, 1246, 1875, 2221, 2352
MbiI	CCGCTC	1 cut(s)	206
MboI	GATC	7 cut(s)	714, 735, 881, 1244, 1873, 2219, 2350
MflI	RGATCY	1 cut(s)	2350
MhlI	GDGCHC	3 cut(s)	150, 726, 2345
MlsI	TGGCCA	1 cut(s)	2228
MluCI	AATT	9 cut(s)	21, 248, 650, 1062, 1197, 1450, 2021, 2155, 2378
MluNI	TGGCCA	1 cut(s)	2228
MlyI	GAGTC	3 cut(s)	293, 316, 2035
MmeI	TCCRAC	3 cut(s)	381, 1547, 2355
Mox20I	TGGCCA	1 cut(s)	2228
MroNI	GCCGGC	1 cut(s)	454
MroXI	GAANNNNTTC	1 cut(s)	2115
MscI	TGGCCA	1 cut(s)	2228
MseI	TTAA	8 cut(s)	389, 1359, 1454, 1602, 1847, 2024, 2213, 2482
MslI	CAYNNNNRTG	2 cut(s)	559, 899
Msp20I	TGGCCA	1 cut(s)	2228
MspA1I	CMGCKG	2 cut(s)	496, 992
MspCI	CTTAAG	1 cut(s)	388
MspI	CCGG	7 cut(s)	376, 455, 508, 512, 732, 1236, 1915
MspR9I	CCNGG	8 cut(s)	86, 190, 733, 743, 1203, 1237, 1916, 2338
Mva1269I	GAATGC	1 cut(s)	1759
MvaI	CCWGG	5 cut(s)	86, 190, 743, 1203, 2338
MvnI	CGCG	2 cut(s)	17, 356
NaeI	GCCGGC	1 cut(s)	456
NciI	CCSGG	3 cut(s)	733, 1237, 1916
NdeII	GATC	7 cut(s)	714, 735, 881, 1244, 1873, 2219, 2350
NgoMIV	GCCGGC	1 cut(s)	454
NlaIII	CATG	8 cut(s)	774, 809, 829, 898, 1087, 1990, 2233, 2243
NlaIV	GGNNCC	5 cut(s)	90, 506, 1863, 1864, 2189
NspI	RCATGY	1 cut(s)	1990
PaeR7I	CTCGAG	1 cut(s)	2344
PceI	AGGCCT	1 cut(s)	2182
PciSI	GCTCTTC	1 cut(s)	530
PcsI	WCGNNNNNNNCGW	1 cut(s)	1693
PctI	GAATGC	1 cut(s)	1759
PdiI	GCCGGC	1 cut(s)	456
PdmI	GAANNNNTTC	1 cut(s)	2115
PfoI	TCCNGGA	1 cut(s)	2336
PkrI	GCNGC	8 cut(s)	180, 236, 460, 495, 681, 1537, 1540, 1618
PleI	GAGTC	3 cut(s)	292, 316, 2035
PpsI	GAGTC	3 cut(s)	292, 316, 2035
Ppu21I	YACGTR	2 cut(s)	1958, 2428
PpuMI	RGGWCCY	2 cut(s)	926, 1862
PshBI	ATTAAT	2 cut(s)	1847, 2024
Psp124BI	GAGCTC	1 cut(s)	2345
Psp5II	RGGWCCY	2 cut(s)	926, 1862
Psp6I	CCWGG	5 cut(s)	84, 188, 741, 1201, 2336
PspGI	CCWGG	5 cut(s)	84, 188, 741, 1201, 2336
PspN4I	GGNNCC	5 cut(s)	90, 506, 1863, 1864, 2189
PspPI	GGNCC	8 cut(s)	88, 192, 225, 505, 926, 1034, 1130, 1862
PspPPI	RGGWCCY	2 cut(s)	926, 1862
PsuI	RGATCY	1 cut(s)	2350
PvuII	CAGCTG	1 cut(s)	992
RsaI	GTAC	4 cut(s)	329, 905, 1156, 1164
RsaNI	GTAC	4 cut(s)	328, 904, 1155, 1163
RseI	CAYNNNNRTG	2 cut(s)	559, 899
SacI	GAGCTC	1 cut(s)	2345
SapI	GCTCTTC	1 cut(s)	530
SaqAI	TTAA	8 cut(s)	389, 1359, 1454, 1602, 1847, 2024, 2213, 2482
SatI	GCNGC	8 cut(s)	179, 235, 459, 494, 680, 1536, 1539, 1617
Sau3AI	GATC	7 cut(s)	714, 735, 881, 1244, 1873, 2219, 2350
Sau96I	GGNCC	8 cut(s)	88, 192, 225, 505, 926, 1034, 1130, 1862
ScaI	AGTACT	1 cut(s)	1164
SchI	GAGTC	3 cut(s)	293, 316, 2035
ScrFI	CCNGG	8 cut(s)	86, 190, 733, 743, 1203, 1237, 1916, 2338
SduI	GDGCHC	3 cut(s)	150, 726, 2345
SfaNI	GCATC	5 cut(s)	117, 1012, 1165, 1230, 1603
Sfr274I	CTCGAG	1 cut(s)	2344
SinI	GGWCC	5 cut(s)	505, 926, 1034, 1130, 1862
SlaI	CTCGAG	1 cut(s)	2344
SmiMI	CAYNNNNRTG	2 cut(s)	559, 899
SmlI	CTYRAG	6 cut(s)	388, 797, 1680, 2176, 2298, 2344
SmoI	CTYRAG	6 cut(s)	388, 797, 1680, 2176, 2298, 2344
SpeI	ACTAGT	1 cut(s)	2014
Sse9I	AATT	9 cut(s)	21, 248, 650, 1062, 1197, 1450, 2021, 2155, 2378
SseBI	AGGCCT	1 cut(s)	2182
SspI	AATATT	1 cut(s)	1487
SspMI	CTAG	6 cut(s)	752, 1787, 2015, 2067, 2184, 2270
SstI	GAGCTC	1 cut(s)	2345
StuI	AGGCCT	1 cut(s)	2182
StyD4I	CCNGG	8 cut(s)	84, 188, 731, 741, 1201, 1235, 1914, 2336
StyI	CCWWGG	5 cut(s)	209, 642, 726, 1315, 2183
TaaI	ACNGT	4 cut(s)	99, 440, 946, 1505
TaiI	ACGT	3 cut(s)	1138, 1960, 2430
TaqI	TCGA	5 cut(s)	320, 856, 1776, 1980, 2345
TasI	AATT	9 cut(s)	21, 248, 650, 1062, 1197, 1450, 2021, 2155, 2378
TatI	WGTACW	1 cut(s)	1162
TauI	GCSGC	2 cut(s)	461, 682
Tru1I	TTAA	8 cut(s)	389, 1359, 1454, 1602, 1847, 2024, 2213, 2482
Tru9I	TTAA	8 cut(s)	389, 1359, 1454, 1602, 1847, 2024, 2213, 2482
TscAI	CASTG	6 cut(s)	42, 456, 724, 1638, 1894, 2229
TseI	GCWGC	6 cut(s)	178, 234, 493, 1535, 1538, 1616
TspDTI	ATGAA	9 cut(s)	842, 1067, 1946, 2306, 2354, 2412, 2424, 2430, 2469
TspGWI	ACGGA	5 cut(s)	121, 627, 797, 1103, 1294
TspRI	CASTG	6 cut(s)	42, 456, 724, 1638, 1894, 2229
Vha464I	CTTAAG	1 cut(s)	388
VpaK11BI	GGWCC	5 cut(s)	505, 926, 1034, 1130, 1862
VspI	ATTAAT	2 cut(s)	1847, 2024
XapI	RAATTY	3 cut(s)	21, 1062, 1450
XceI	RCATGY	1 cut(s)	1990
XcmI	CCANNNNNNNNNTGG	1 cut(s)	947
XhoI	CTCGAG	1 cut(s)	2344
XmaJI	CCTAGG	1 cut(s)	2183
XmiI	GTMKAC	1 cut(s)	2095
XmnI	GAANNNNTTC	1 cut(s)	2115
XspI	CTAG	6 cut(s)	752, 1787, 2015, 2067, 2184, 2270
ZrmI	AGTACT	1 cut(s)	1164

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.