Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0009432

Physical Location & Seq

Genomic Coordinates Reverse (-)

5277296 .. 5283627

Gene Structure 6332 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G006220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2394 bp

ATGGCCTTCGCAACCGCCAAAATTTCCGAAATGCCACTCAGAAACTCACTCCCGCTCACCTCTCACTCCCCTTCCTCTCTCAACCTCCTCTTCTCGTCCCCAAAACCGTCATTTCACATCCTCAAGCCTTTCTCTGCTCTCAGAACCACCGAGCACGGCGGCAACCCGAACGCGAGGCCGTCGCCGCACAAGTCCAAGCCCTCGTCGTCCTCCTCCACCGCGCCGTGGCTGAATAAGTGGCCGTCTCGCGGTGCTCCCTCGGAGTTGCCCCGCCATAAGGTCAACGATAGAGTCAAGGAGTCCAAGGGCGGTGGAGAGAAGCCGAGTAATGGCGCTAGGTACGTGGACAAGGACAAAGGCCAGAGCGCGATAGAGAGGATCGTGTTCCGGTTGCGGAACCTAGGGTTAGGCGACGAAGACGAGGAGGAGGAGGAGAGCGGTGATGGTGTTGAATTGGACGGTCCGGATTCAATGCCGGCGGCGAGCGGAGAGGAGAAGCTCGGCGATTTGCTGCAGCGGGAGTGGGTCCGGCCGGACTATATATTGGTGGAGGAGAAGGGCGACGATGACGTGGCGCTGCCATGGGAGAAGGAGGAGGAGGAGGAGGAATTGAGTGAGGATGAGGAGGTGAAGGGGCTGAGGAGAGCTCGGAGGTCGAAGGCGCCGAGCTTGGCTGAGCTGACGATTGAGGATGAGGAGCTGAGGCGATTGAGGAGGTTGGGAATGTTCCTGAGGGAGAGGATCAGTGTGCCGAAAGCCGGGATTACGCAGGCTGTGCTGGAGAAGATTCATGATAAGTGGAGGAAGGAGGAGCTGGTGAGGCTCAAGTTTCATGAGGTTTTGGCTCATGATATGAAGACTGCTCATGAGATTGTTGAGCGTCGAACAGGAGGTTTAGTTTTATGGAGATCAGGGAGTGTCATGGTTGTGTACCGTGGGATTAACTACAAAGGCCCTTCTAAATCCGAACCCGTTGGTAGGGAAGGAAATGCTCTTTTCATCCCAGATGTTTCATCAGCTGAAACTCCGGTAACAAGAAGTGGCAATGATGTGACTTCAACTCCAGATAAGACTGAGCAAGCTGTGAAAATTCCAGCACCTCTTCCGAAGATGACAGAGGAAGAAGCTGAATTCAACAGCTTATTGGATGAATTAGGTCCTCGTTTTGTTGAGTATTGGGGTACAGGAATACTTCCTGTTGACGCTGATTTACTTCCTAAAACAGTCCCGGGTTACAAAACACCTTTCAGGCTTCTTCCTACCGGGATGCGATCACGGCTGACTAATGCAGAGATGACTAATTTGCGGAAACTTGCTAAATCAATTCCATGTCATTTTGCCCTTGGGAGAAATAGAAATCATCAAGGGTTAGCATCTGCAATACTTAAAGTTTGGGAGAAAAGTTCTGTGGCGAAGATTGCTGTCAAACGAGGTATCCAGAATACAAACAACAAAATAATGGCTGAGGAGCTGAAGGCATTAACAGGTGGTGTTTTACTACTCAGAAACAAATATTACATTGTCATTTACCGTGGAAAGGACTTTATCCCAACAAGTGTAGCTGCTGCTCTGGCTGAAAGACAGGAACTAACGAAACAGGTTCAAGATGTGGAAGAGATAGTGCGTGTCAAACCAATAGATGCAGCTTCTTCAGGTACAGAGGAAGGACAGGCACTTGCAGGAACTTTGGCAGAGTTCTACGAGGCCCAAGCCCGATGGGGAAGAGAAATATCTGCTGAAGAACGTGAAAAGATGATTGAAGAAGATTCAAAAGCTAAGATTGCCAGGCGTGCAAAACGAATTGAACATAAGCTAGGCGTTGCCCAAGCTAAAAAGCTTAGAGCAGAGAGACTCCTATCCAAGATAGAATCAGCAATGCTTCCTGCAGGTCCTGATTATGATCAGGAAACCATCACTGATGAGGAGCGGGTTATGTTTCGCAGGGTTGGCTTAAGAATGAAAGCATACTTGCCTCTTGGCATACGTGGTGTCTTTGATGGTGTCATCGAAAACATGCATTTGCATTGGAAGCACAGAGAGCTAGTGAAATTAATATCCAAACAGAAGACCCTTGCTTTCCCGAATTATAGGCGTCCCATTACCTTGAGGCCAAGGAACCTTTTAACTAAGGCGAAGGCATTGAAACGTTCGGTGGCCATGCAACGCCATGAGGCTCTCAGTCAGCACATCGGGGAATTGGAGAGGACCATAGAGCAAATGAGATCTGAAATTGGTATTTCTGAAGATGTAGAAAATGAGAGCACTTGGACCTCAAGAAATCCCCATCAGAGTGGTCATGACTCGGAATTTAACCAAAGTGAGGATGAAGCTTCTGACATGGAATCAGATGGTAGTGACGATGATGAAGGCTCAGATTGGGAAGATGAGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRS1_YhbY

Protein Analysis

797

Amino Acids

89.64

Weight (kDa)

6.19

Isoelectric Point (pI)

60.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRS1_YhbY	PF01985	230 - 312	4.4e-20	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	427 - 511	1.6e-15	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	638 - 689	5.4e-10	CRS1 / YhbY (CRM) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009432)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18390
fragaria_vesca	FvH4_5g12070 FvH4_5g12070 FvH4_5g12070 FvH4_5g12070
malus_domestica	MD06G1216600.v1.1 MD14G1227100.v1.1
prunus_persica	Prupe.5G221800_v2.0.a1
pyrus_communis	pycom06g19300 pycom14g18850
rosa_chinensis	RchiOBHm_Chr7g0185501
rosa_laevigata	RLG00000004907
rosa_multiflora	Rmu_sc0008223.1_g000008 Rmu_sc0010190.1_g000020 Rmu_sc0042727.1_g000001
rosa_roxburghii	Rroxscaffold_3G00268880
rosa_rugosa	Rorug06G0469800
rosa_samantha	Rh7AG072900 Rh7BG074700 Rh7CG074800 Rh7DG074700
rosa_wichuraiana	Rw7G006220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1877
AccB1I	GGYRCC	1 cut(s)	661
AccBSI	CCGCTC	4 cut(s)	55, 438, 486, 1927
AccII	CGCG	4 cut(s)	173, 221, 249, 368
AccIII	TCCGGA	1 cut(s)	463
AclI	AACGTT	1 cut(s)	2146
AclWI	GGATC	2 cut(s)	386, 749
AcoI	YGGCCR	3 cut(s)	239, 530, 2154
AcsI	RAATTY	4 cut(s)	21, 1089, 1130, 2306
AcuI	CTGAAG	4 cut(s)	1493, 1635, 1758, 2262
AcyI	GRCGYC	2 cut(s)	662, 2092
AfaI	GTAC	4 cut(s)	341, 932, 1183, 1657
AfiI	CCNNNNNNNGG	8 cut(s)	225, 248, 277, 329, 758, 978, 1537, 2320
AflII	CTTAAG	1 cut(s)	1951
AgsI	TTSAA	9 cut(s)	452, 471, 1059, 1135, 1604, 1760, 1770, 1805, 2143
AjiI	CACGTC	1 cut(s)	571
AjnI	CCWGG	1 cut(s)	1784
Alw21I	GWGCWC	4 cut(s)	156, 256, 649, 2264
Alw26I	GTCTC	2 cut(s)	249, 1843
AlwI	GGATC	2 cut(s)	386, 749
AlwNI	CAGNNNCTG	1 cut(s)	1892
Ama87I	CYCGRG	1 cut(s)	1228
Aor13HI	TCCGGA	1 cut(s)	463
AoxI	GGCC	9 cut(s)	3, 176, 239, 358, 530, 952, 1704, 2108, 2154
ApeKI	GCWGC	6 cut(s)	511, 514, 577, 1562, 1565, 1643
ApoI	RAATTY	4 cut(s)	21, 1089, 1130, 2306
AseI	ATTAAT	1 cut(s)	2051
AspA2I	CCTAGG	1 cut(s)	400
AspLEI	GCGC	5 cut(s)	223, 335, 368, 577, 664
AspS9I	GGNCC	8 cut(s)	461, 526, 953, 1157, 1705, 1889, 2205, 2268
AsuC2I	CCSGG	4 cut(s)	760, 1229, 1230, 1264
AsuHPI	GGTGA	4 cut(s)	49, 452, 640, 829
AvaI	CYCGRG	1 cut(s)	1228
AvaII	GGWCC	6 cut(s)	461, 526, 1157, 1889, 2205, 2268
AvrII	CCTAGG	1 cut(s)	400
AxyI	CCTNAGG	1 cut(s)	731
BalI	TGGCCA	1 cut(s)	2156
BanI	GGYRCC	1 cut(s)	661
BanII	GRGCYC	1 cut(s)	649
BbsI	GAAGAC	3 cut(s)	423, 863, 2072
Bbv12I	GWGCWC	4 cut(s)	156, 256, 649, 2264
BbvCI	CCTCAGC	3 cut(s)	638, 701, 1464
BbvI	GCAGC	6 cut(s)	498, 526, 564, 1549, 1552, 1655
BccI	CCATC	6 cut(s)	437, 1710, 1919, 1991, 2292, 2342
BceAI	ACGGC	5 cut(s)	163, 172, 208, 226, 1292
BciT130I	CCWGG	1 cut(s)	1786
BciVI	GTATCC	1 cut(s)	1445
BclI	TGATCA	1 cut(s)	1900
BcnI	CCSGG	4 cut(s)	760, 1229, 1230, 1264
BcoDI	GTCTC	2 cut(s)	249, 1843
BfaI	CTAG	4 cut(s)	336, 401, 1814, 2042
BfmI	CTRYAG	2 cut(s)	512, 1884
BfoI	RGCGCY	3 cut(s)	336, 578, 665
BfrI	CTTAAG	1 cut(s)	1951
BfuAI	ACCTGC	1 cut(s)	1877
BfuI	GTATCC	1 cut(s)	1445
BglII	AGATCT	1 cut(s)	2222
BisI	GCNGC	9 cut(s)	160, 185, 480, 512, 515, 578, 1563, 1566, 1644
BlnI	CCTAGG	1 cut(s)	400
BlpI	GCTNAGC	1 cut(s)	675
BlsI	GCNGC	9 cut(s)	161, 186, 481, 513, 516, 579, 1564, 1567, 1645
Bme1390I	CCNGG	5 cut(s)	760, 1229, 1230, 1264, 1786
Bme18I	GGWCC	6 cut(s)	461, 526, 1157, 1889, 2205, 2268
BmeT110I	CYCGRG	1 cut(s)	1228
BmgBI	CACGTC	1 cut(s)	571
BmgT120I	GGNCC	8 cut(s)	461, 526, 953, 1157, 1705, 1889, 2205, 2268
BmiI	GGNNCC	4 cut(s)	398, 527, 663, 2117
BmrFI	CCNGG	5 cut(s)	760, 1229, 1230, 1264, 1786
BmsI	GCATC	3 cut(s)	1257, 1382, 1630
BpiI	GAAGAC	3 cut(s)	423, 863, 2072
BplI	GAGNNNNNCTC	2 cut(s)	631, 663
BpmI	CTGGAG	2 cut(s)	800, 1047
Bpu10I	CCTNAGC	3 cut(s)	638, 701, 1464
Bpu1102I	GCTNAGC	1 cut(s)	675
BpuEI	CTTGAG	4 cut(s)	107, 809, 2125, 2257
BpuMI	CCSGG	4 cut(s)	760, 1229, 1230, 1264
BsaAI	YACGTR	2 cut(s)	343, 1985
BsaBI	GATNNNNATC	1 cut(s)	1899
BsaHI	GRCGYC	2 cut(s)	662, 2092
BsaWI	WCCGGW	3 cut(s)	387, 463, 1027
BsaXI	ACNNNNNCTCC	4 cut(s)	882, 912, 1045, 1075
Bsc4I	CCNNNNNNNGG	8 cut(s)	225, 248, 277, 329, 758, 978, 1537, 2320
Bse118I	RCCGGY	1 cut(s)	475
Bse21I	CCTNAGG	1 cut(s)	731
Bse3DI	GCAATG	2 cut(s)	1051, 1881
Bse8I	GATNNNNATC	1 cut(s)	1899
BseAI	TCCGGA	1 cut(s)	463
BseBI	CCWGG	1 cut(s)	1786
BseGI	GGATG	7 cut(s)	117, 625, 697, 999, 1153, 1272, 2329
BseJI	GATNNNNATC	1 cut(s)	1899
BseLI	CCNNNNNNNGG	8 cut(s)	225, 248, 277, 329, 758, 978, 1537, 2320
BseMI	GCAATG	2 cut(s)	1051, 1881
BseX3I	CGGCCG	1 cut(s)	530
BseXI	GCAGC	6 cut(s)	498, 526, 564, 1549, 1552, 1655
Bsh1236I	CGCG	4 cut(s)	173, 221, 249, 368
Bsh1285I	CGRYCG	1 cut(s)	533
BshFI	GGCC	9 cut(s)	5, 178, 241, 360, 532, 954, 1706, 2110, 2156
BshNI	GGYRCC	1 cut(s)	661
BsiEI	CGRYCG	1 cut(s)	533
BsiHKAI	GWGCWC	4 cut(s)	156, 256, 649, 2264
BsiHKCI	CYCGRG	1 cut(s)	1228
BsiSI	CCGG	9 cut(s)	388, 464, 476, 529, 533, 759, 1028, 1229, 1263
BslFI	GGGAC	3 cut(s)	82, 1211, 2079
BslI	CCNNNNNNNGG	8 cut(s)	225, 248, 277, 329, 758, 978, 1537, 2320
BsmAI	GTCTC	2 cut(s)	249, 1843
BsmBI	CGTCTC	1 cut(s)	249
BsmFI	GGGAC	3 cut(s)	82, 1211, 2079
BsnI	GGCC	9 cut(s)	5, 178, 241, 360, 532, 954, 1706, 2110, 2156
BsoBI	CYCGRG	1 cut(s)	1228
Bsp1286I	GDGCHC	4 cut(s)	156, 256, 649, 2264
Bsp13I	TCCGGA	1 cut(s)	463
Bsp143I	GATC	6 cut(s)	378, 741, 908, 1271, 1900, 2222
Bsp1720I	GCTNAGC	1 cut(s)	675
Bsp19I	CCATGG	1 cut(s)	581
BspANI	GGCC	9 cut(s)	5, 178, 241, 360, 532, 954, 1706, 2110, 2156
BspEI	TCCGGA	1 cut(s)	463
BspFNI	CGCG	4 cut(s)	173, 221, 249, 368
BspHI	TCATGA	5 cut(s)	790, 832, 847, 865, 2296
BspLI	GGNNCC	4 cut(s)	398, 527, 663, 2117
BspMAI	CTGCAG	2 cut(s)	516, 1888
BspMI	ACCTGC	1 cut(s)	1877
BspPI	GGATC	2 cut(s)	386, 749
BspT107I	GGYRCC	1 cut(s)	661
BspTI	CTTAAG	1 cut(s)	1951
BsrBI	CCGCTC	4 cut(s)	55, 438, 486, 1927
BsrDI	GCAATG	2 cut(s)	1051, 1881
BsrFI	RCCGGY	1 cut(s)	475
BssAI	RCCGGY	1 cut(s)	475
BssMI	GATC	6 cut(s)	378, 741, 908, 1271, 1900, 2222
BssNI	GRCGYC	2 cut(s)	662, 2092
BssT1I	CCWWGG	5 cut(s)	303, 400, 581, 1342, 2111
Bst2UI	CCWGG	1 cut(s)	1786
Bst4CI	ACNGT	5 cut(s)	108, 461, 935, 1225, 1532
Bst6I	CTCTTC	4 cut(s)	95, 1107, 1608, 1717
BstACI	GRCGYC	2 cut(s)	662, 2092
BstAFI	CTTAAG	1 cut(s)	1951
BstAPI	GCANNNNNTGC	1 cut(s)	775
BstBAI	YACGTR	2 cut(s)	343, 1985
BstC8I	GCNNGC	5 cut(s)	477, 484, 771, 1080, 1791
BstDSI	CCRYGG	4 cut(s)	224, 581, 934, 1531
BstF5I	GGATG	7 cut(s)	117, 625, 697, 999, 1153, 1272, 2329
BstFNI	CGCG	4 cut(s)	173, 221, 249, 368
BstH2I	RGCGCY	3 cut(s)	336, 578, 665
BstHHI	GCGC	5 cut(s)	223, 335, 368, 577, 664
BstKTI	GATC	6 cut(s)	381, 744, 911, 1274, 1903, 2225
BstMAI	GTCTC	2 cut(s)	249, 1843
BstMBI	GATC	6 cut(s)	378, 741, 908, 1271, 1900, 2222
BstMCI	CGRYCG	1 cut(s)	533
BstNI	CCWGG	1 cut(s)	1786
BstNSI	RCATGY	1 cut(s)	2017
BstSCI	CCNGG	5 cut(s)	758, 1227, 1228, 1262, 1784
BstSFI	CTRYAG	2 cut(s)	512, 1884
BstUI	CGCG	4 cut(s)	173, 221, 249, 368
BstV1I	GCAGC	6 cut(s)	498, 526, 564, 1549, 1552, 1655
BstV2I	GAAGAC	3 cut(s)	423, 863, 2072
BstX2I	RGATCY	1 cut(s)	2222
BstXI	CCANNNNNNTGG	1 cut(s)	2291
BstYI	RGATCY	1 cut(s)	2222
BstZI	CGGCCG	1 cut(s)	530
Bsu36I	CCTNAGG	1 cut(s)	731
BsuI	GTATCC	1 cut(s)	1445
BsuRI	GGCC	9 cut(s)	5, 178, 241, 360, 532, 954, 1706, 2110, 2156
BtgI	CCRYGG	4 cut(s)	224, 581, 934, 1531
BtrI	CACGTC	1 cut(s)	571
BtsCI	GGATG	7 cut(s)	117, 625, 697, 999, 1153, 1272, 2329
BtsIMutI	CAGTG	2 cut(s)	751, 1914
BveI	ACCTGC	1 cut(s)	1877
Cac8I	GCNNGC	5 cut(s)	477, 484, 771, 1080, 1791
CaiI	CAGNNNCTG	1 cut(s)	1892
CciI	TCATGA	5 cut(s)	790, 832, 847, 865, 2296
CfoI	GCGC	5 cut(s)	223, 335, 368, 577, 664
Cfr10I	RCCGGY	1 cut(s)	475
Cfr13I	GGNCC	8 cut(s)	461, 526, 953, 1157, 1705, 1889, 2205, 2268
Cfr9I	CCCGGG	1 cut(s)	1228
CpoI	CGGWCCG	1 cut(s)	461
CseI	GACGC	3 cut(s)	869, 1211, 2081
Csp6I	GTAC	4 cut(s)	340, 931, 1182, 1656
CspCI	CAANNNNNGTGG	2 cut(s)	292, 327
CspI	CGGWCCG	1 cut(s)	461
CviQI	GTAC	4 cut(s)	340, 931, 1182, 1656
DinI	GGCGCC	1 cut(s)	663
DpnI	GATC	6 cut(s)	380, 743, 910, 1273, 1902, 2224
DpnII	GATC	6 cut(s)	378, 741, 908, 1271, 1900, 2222
EaeI	YGGCCR	3 cut(s)	239, 530, 2154
EagI	CGGCCG	1 cut(s)	530
Eam1104I	CTCTTC	4 cut(s)	95, 1107, 1608, 1717
EarI	CTCTTC	4 cut(s)	95, 1107, 1608, 1717
Ecl136II	GAGCTC	1 cut(s)	647
EclXI	CGGCCG	1 cut(s)	530
Eco130I	CCWWGG	5 cut(s)	303, 400, 581, 1342, 2111
Eco24I	GRGCYC	1 cut(s)	649
Eco47I	GGWCC	6 cut(s)	461, 526, 1157, 1889, 2205, 2268
Eco52I	CGGCCG	1 cut(s)	530
Eco53kI	GAGCTC	1 cut(s)	647
Eco57I	CTGAAG	4 cut(s)	1493, 1635, 1758, 2262
Eco81I	CCTNAGG	1 cut(s)	731
Eco88I	CYCGRG	1 cut(s)	1228
EcoICRI	GAGCTC	1 cut(s)	647
EcoO109I	RGGNCCY	3 cut(s)	953, 1157, 1889
EcoRI	GAATTC	1 cut(s)	1130
EcoRII	CCWGG	1 cut(s)	1784
EcoT14I	CCWWGG	5 cut(s)	303, 400, 581, 1342, 2111
EcoT22I	ATGCAT	1 cut(s)	2019
EcoT38I	GRGCYC	1 cut(s)	649
EgeI	GGCGCC	1 cut(s)	663
EheI	GGCGCC	1 cut(s)	663
ErhI	CCWWGG	5 cut(s)	303, 400, 581, 1342, 2111
Esp3I	CGTCTC	1 cut(s)	249
FaqI	GGGAC	3 cut(s)	82, 1211, 2079
FauI	CCCGC	4 cut(s)	60, 278, 510, 1920
FbaI	TGATCA	1 cut(s)	1900
Fnu4HI	GCNGC	9 cut(s)	160, 185, 480, 512, 515, 578, 1563, 1566, 1644
FokI	GGATG	7 cut(s)	104, 632, 704, 986, 1160, 1279, 2336
FriOI	GRGCYC	1 cut(s)	649
Fsp4HI	GCNGC	9 cut(s)	160, 185, 480, 512, 515, 578, 1563, 1566, 1644
FspBI	CTAG	4 cut(s)	336, 401, 1814, 2042
GlaI	GCGC	5 cut(s)	222, 334, 367, 576, 663
GluI	GCNGC	9 cut(s)	160, 185, 480, 512, 515, 578, 1563, 1566, 1644
GsuI	CTGGAG	2 cut(s)	800, 1047
HaeII	RGCGCY	3 cut(s)	336, 578, 665
HaeIII	GGCC	9 cut(s)	5, 178, 241, 360, 532, 954, 1706, 2110, 2156
HapII	CCGG	9 cut(s)	388, 464, 476, 529, 533, 759, 1028, 1229, 1263
HgaI	GACGC	3 cut(s)	869, 1211, 2081
HhaI	GCGC	5 cut(s)	223, 335, 368, 577, 664
Hin1I	GRCGYC	2 cut(s)	662, 2092
Hin6I	GCGC	5 cut(s)	221, 333, 366, 575, 662
HinP1I	GCGC	5 cut(s)	221, 333, 366, 575, 662
HincII	GTYRAC	2 cut(s)	283, 1201
HindII	GTYRAC	2 cut(s)	283, 1201
HindIII	AAGCTT	2 cut(s)	1835, 2328
HinfI	GANTC	9 cut(s)	291, 299, 467, 787, 1766, 1851, 1868, 2300, 2342
HpaII	CCGG	9 cut(s)	388, 464, 476, 529, 533, 759, 1028, 1229, 1263
HphI	GGTGA	4 cut(s)	49, 452, 640, 829
Hpy166II	GTNNAC	4 cut(s)	283, 346, 931, 1201
Hpy8I	GTNNAC	4 cut(s)	283, 346, 931, 1201
Hpy99I	CGWCG	5 cut(s)	184, 208, 416, 566, 885
HpyCH4III	ACNGT	5 cut(s)	108, 461, 935, 1225, 1532
HpyCH4IV	ACGT	5 cut(s)	342, 570, 1744, 1984, 2146
HpySE526I	ACGT	5 cut(s)	342, 570, 1744, 1984, 2146
Hsp92I	GRCGYC	2 cut(s)	662, 2092
HspAI	GCGC	5 cut(s)	221, 333, 366, 575, 662
KasI	GGCGCC	1 cut(s)	661
Kpn2I	TCCGGA	1 cut(s)	463
KroI	GCCGGC	1 cut(s)	475
KroNI	GCCGGC	1 cut(s)	477
Ksp22I	TGATCA	1 cut(s)	1900
Kzo9I	GATC	6 cut(s)	378, 741, 908, 1271, 1900, 2222
LmnI	GCTCC	5 cut(s)	259, 697, 811, 1468, 1924
Lsp1109I	GCAGC	6 cut(s)	498, 526, 564, 1549, 1552, 1655
LweI	GCATC	3 cut(s)	1257, 1382, 1630
MaeI	CTAG	4 cut(s)	336, 401, 1814, 2042
MaeII	ACGT	5 cut(s)	342, 570, 1744, 1984, 2146
MaeIII	GTNAC	4 cut(s)	1030, 1051, 1232, 2354
MalI	GATC	6 cut(s)	380, 743, 910, 1273, 1902, 2224
MbiI	CCGCTC	4 cut(s)	55, 438, 486, 1927
MboI	GATC	6 cut(s)	378, 741, 908, 1271, 1900, 2222
MflI	RGATCY	1 cut(s)	2222
MhlI	GDGCHC	4 cut(s)	156, 256, 649, 2264
MlsI	TGGCCA	1 cut(s)	2156
MluNI	TGGCCA	1 cut(s)	2156
Mly113I	GGCGCC	1 cut(s)	662
MlyI	GAGTC	4 cut(s)	300, 308, 1845, 2294
Mox20I	TGGCCA	1 cut(s)	2156
Mph1103I	ATGCAT	1 cut(s)	2019
MroI	TCCGGA	1 cut(s)	463
MroNI	GCCGGC	1 cut(s)	475
MscI	TGGCCA	1 cut(s)	2156
MseI	TTAA	8 cut(s)	942, 1386, 1481, 1952, 2051, 2123, 2310, 2392
MslI	CAYNNNNRTG	2 cut(s)	926, 2289
Msp20I	TGGCCA	1 cut(s)	2156
MspA1I	CMGCKG	2 cut(s)	517, 1019
MspCI	CTTAAG	1 cut(s)	1951
MspI	CCGG	9 cut(s)	388, 464, 476, 529, 533, 759, 1028, 1229, 1263
MspR9I	CCNGG	5 cut(s)	760, 1229, 1230, 1264, 1786
MvaI	CCWGG	1 cut(s)	1786
MvnI	CGCG	4 cut(s)	173, 221, 249, 368
NaeI	GCCGGC	1 cut(s)	477
NarI	GGCGCC	1 cut(s)	662
NciI	CCSGG	4 cut(s)	760, 1229, 1230, 1264
NcoI	CCATGG	1 cut(s)	581
NdeII	GATC	6 cut(s)	378, 741, 908, 1271, 1900, 2222
NgoMIV	GCCGGC	1 cut(s)	475
NlaIV	GGNNCC	4 cut(s)	398, 527, 663, 2117
NmeAIII	GCCGAG	3 cut(s)	348, 480, 690
NmuCI	GTSAC	2 cut(s)	1051, 2354
NsiI	ATGCAT	1 cut(s)	2019
NspI	RCATGY	1 cut(s)	2017
PagI	TCATGA	5 cut(s)	790, 832, 847, 865, 2296
PcsI	WCGNNNNNNNCGW	1 cut(s)	662
PdiI	GCCGGC	1 cut(s)	477
PfeI	GAWTC	5 cut(s)	467, 787, 1766, 1868, 2342
PkrI	GCNGC	9 cut(s)	161, 186, 481, 513, 516, 579, 1564, 1567, 1645
PleI	GAGTC	4 cut(s)	299, 307, 1845, 2294
PluTI	GGCGCC	1 cut(s)	665
PpsI	GAGTC	4 cut(s)	299, 307, 1845, 2294
Ppu21I	YACGTR	2 cut(s)	343, 1985
PpuMI	RGGWCCY	2 cut(s)	1157, 1889
PshBI	ATTAAT	1 cut(s)	2051
Psp124BI	GAGCTC	1 cut(s)	649
Psp1406I	AACGTT	1 cut(s)	2146
Psp5II	RGGWCCY	2 cut(s)	1157, 1889
Psp6I	CCWGG	1 cut(s)	1784
PspGI	CCWGG	1 cut(s)	1784
PspN4I	GGNNCC	4 cut(s)	398, 527, 663, 2117
PspPI	GGNCC	8 cut(s)	461, 526, 953, 1157, 1705, 1889, 2205, 2268
PspPPI	RGGWCCY	2 cut(s)	1157, 1889
PstI	CTGCAG	2 cut(s)	516, 1888
PstNI	CAGNNNCTG	1 cut(s)	1892
PsuI	RGATCY	1 cut(s)	2222
PvuII	CAGCTG	1 cut(s)	1019
RsaI	GTAC	4 cut(s)	341, 932, 1183, 1657
RsaNI	GTAC	4 cut(s)	340, 931, 1182, 1656
RseI	CAYNNNNRTG	2 cut(s)	926, 2289
Rsr2I	CGGWCCG	1 cut(s)	461
RsrII	CGGWCCG	1 cut(s)	461
SacI	GAGCTC	1 cut(s)	649
SaqAI	TTAA	8 cut(s)	942, 1386, 1481, 1952, 2051, 2123, 2310, 2392
SatI	GCNGC	9 cut(s)	160, 185, 480, 512, 515, 578, 1563, 1566, 1644
Sau3AI	GATC	6 cut(s)	378, 741, 908, 1271, 1900, 2222
Sau96I	GGNCC	8 cut(s)	461, 526, 953, 1157, 1705, 1889, 2205, 2268
SbfI	CCTGCAGG	1 cut(s)	1888
SchI	GAGTC	4 cut(s)	300, 308, 1845, 2294
ScrFI	CCNGG	5 cut(s)	760, 1229, 1230, 1264, 1786
SdaI	CCTGCAGG	1 cut(s)	1888
SduI	GDGCHC	4 cut(s)	156, 256, 649, 2264
SfaNI	GCATC	3 cut(s)	1257, 1382, 1630
SfcI	CTRYAG	2 cut(s)	512, 1884
SfoI	GGCGCC	1 cut(s)	663
SinI	GGWCC	6 cut(s)	461, 526, 1157, 1889, 2205, 2268
SmaI	CCCGGG	1 cut(s)	1230
SmiMI	CAYNNNNRTG	2 cut(s)	926, 2289
SmlI	CTYRAG	5 cut(s)	122, 824, 1951, 2104, 2272
SmoI	CTYRAG	5 cut(s)	122, 824, 1951, 2104, 2272
Sse8387I	CCTGCAGG	1 cut(s)	1888
SspDI	GGCGCC	1 cut(s)	661
SspI	AATATT	1 cut(s)	1514
SspMI	CTAG	4 cut(s)	336, 401, 1814, 2042
SstI	GAGCTC	1 cut(s)	649
StyD4I	CCNGG	5 cut(s)	758, 1227, 1228, 1262, 1784
StyI	CCWWGG	5 cut(s)	303, 400, 581, 1342, 2111
TaaI	ACNGT	5 cut(s)	108, 461, 935, 1225, 1532
TaiI	ACGT	5 cut(s)	345, 573, 1747, 1987, 2149
TaqI	TCGA	3 cut(s)	656, 883, 2007
TauI	GCSGC	3 cut(s)	162, 187, 482
TfiI	GAWTC	5 cut(s)	467, 787, 1766, 1868, 2342
Tru1I	TTAA	8 cut(s)	942, 1386, 1481, 1952, 2051, 2123, 2310, 2392
Tru9I	TTAA	8 cut(s)	942, 1386, 1481, 1952, 2051, 2123, 2310, 2392
TscAI	CASTG	2 cut(s)	751, 1921
TseFI	GTSAC	2 cut(s)	1051, 2354
TseI	GCWGC	6 cut(s)	511, 514, 577, 1562, 1565, 1643
Tsp45I	GTSAC	2 cut(s)	1051, 2354
TspDTI	ATGAA	9 cut(s)	779, 821, 869, 988, 1002, 1164, 1973, 2340, 2379
TspMI	CCCGGG	1 cut(s)	1228
TspRI	CASTG	2 cut(s)	751, 1921
Vha464I	CTTAAG	1 cut(s)	1951
VpaK11BI	GGWCC	6 cut(s)	461, 526, 1157, 1889, 2205, 2268
VspI	ATTAAT	1 cut(s)	2051
XapI	RAATTY	4 cut(s)	21, 1089, 1130, 2306
XceI	RCATGY	1 cut(s)	2017
XmaI	CCCGGG	1 cut(s)	1228
XmaJI	CCTAGG	1 cut(s)	400
XspI	CTAG	4 cut(s)	336, 401, 1814, 2042
Zsp2I	ATGCAT	1 cut(s)	2019

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.