MD07G1000900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010227
Physical Location & Seq
Forward (+)
154612 .. 154941
330 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1000900.v1.1.491

Sequence Viewer

Length: 330 bp
ATGTTTAGAACACCTCTTTATANCCAGGCGCTCCGAACCCGCGGCGGCTCTGCCACCGGTCCGAGCCGGTGGACCAGCCCGGGCCACGAGGAACAACCCAAAGGCTACCTCTTCAATCGGACCCCACCTCCCCCAGGCCAGTCTCGGAAATGGGAGGATTGGGAGCTCCCGTGCTACGTCACCAGCTTCCTCACCATCGTCATCCTCGGCGTCGGCCTCAACGCCAAGCCTGATCTCACCATCGAGACTTGGGCTCACCAGAAAGCCCTCGAACGGCTCGAGATCGAGCAGGCCGCCCTCTCTGTCGCCGGGTCGGGCGAATCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

110

Amino Acids

12.0

Weight (kDa)

6.1

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 42
AciI CCGC 4 cut(s) 40, 42, 45, 294
AcyI GRCGYC 1 cut(s) 210
AfiI CCNNNNNNNGG 2 cut(s) 134, 273
AgeI ACCGGT 1 cut(s) 56
AgsI TTSAA 1 cut(s) 115
AjnI CCWGG 2 cut(s) 24, 133
AluBI AGCT 2 cut(s) 166, 186
AluI AGCT 2 cut(s) 166, 186
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 2 cut(s) 147, 239
Ama87I CYCGRG 2 cut(s) 79, 278
AoxI GGCC 4 cut(s) 82, 136, 214, 291
AsiGI ACCGGT 1 cut(s) 56
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 4 cut(s) 59, 72, 82, 120
AsuC2I CCSGG 3 cut(s) 80, 81, 310
AsuHPI GGTGA 4 cut(s) 172, 184, 229, 248
AvaI CYCGRG 2 cut(s) 79, 278
AvaII GGWCC 3 cut(s) 59, 72, 120
BanII GRGCYC 2 cut(s) 168, 256
BauI CACGAG 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 2 cut(s) 203, 248
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 2 cut(s) 26, 135
BcnI CCSGG 3 cut(s) 80, 81, 310
BcoDI GTCTC 2 cut(s) 147, 239
BfoI RGCGCY 1 cut(s) 32
BisI GCNGC 3 cut(s) 43, 46, 294
BlsI GCNGC 3 cut(s) 44, 47, 295
Bme1390I CCNGG 5 cut(s) 26, 80, 81, 135, 310
Bme18I GGWCC 3 cut(s) 59, 72, 120
BmeT110I CYCGRG 2 cut(s) 79, 278
BmgT120I GGNCC 4 cut(s) 59, 72, 82, 120
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 5 cut(s) 26, 80, 81, 135, 310
BpuMI CCSGG 3 cut(s) 80, 81, 310
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 4 cut(s) 40, 79, 133, 205
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 4 cut(s) 112, 142, 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 273
Bse118I RCCGGY 2 cut(s) 56, 66
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 2 cut(s) 26, 135
BseDI CCNNGG 4 cut(s) 40, 79, 133, 205
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 134, 273
BseNI ACTGG 1 cut(s) 139
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 4 cut(s) 84, 138, 216, 293
BshTI ACCGGT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 2 cut(s) 79, 278
BsiSI CCGG 4 cut(s) 57, 67, 80, 309
BslI CCNNNNNNNGG 2 cut(s) 134, 273
BsmAI GTCTC 2 cut(s) 147, 239
BsnI GGCC 4 cut(s) 84, 138, 216, 293
BsoBI CYCGRG 2 cut(s) 79, 278
Bsp1286I GDGCHC 2 cut(s) 168, 256
Bsp143I GATC 2 cut(s) 232, 282
BspACI CCGC 4 cut(s) 40, 42, 45, 294
BspANI GGCC 4 cut(s) 84, 138, 216, 293
BspFNI CGCG 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 122
BsrFI RCCGGY 2 cut(s) 56, 66
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 2 cut(s) 56, 66
BssECI CCNNGG 4 cut(s) 40, 79, 133, 205
BssMI GATC 2 cut(s) 232, 282
BssNI GRCGYC 1 cut(s) 210
BssSI CACGAG 1 cut(s) 86
Bst2BI CACGAG 1 cut(s) 86
Bst2UI CCWGG 2 cut(s) 26, 135
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 40
BstENI CCTNNNNNAGG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 1 cut(s) 42
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 2 cut(s) 235, 285
BstMAI GTCTC 2 cut(s) 147, 239
BstMBI GATC 2 cut(s) 232, 282
BstNI CCWGG 2 cut(s) 26, 135
BstSCI CCNGG 5 cut(s) 24, 78, 79, 133, 308
BstUI CGCG 1 cut(s) 42
BsuRI GGCC 4 cut(s) 84, 138, 216, 293
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 291
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 2 cut(s) 56, 66
Cfr13I GGNCC 4 cut(s) 59, 72, 82, 120
Cfr42I CCGCGG 1 cut(s) 43
Cfr9I CCCGGG 1 cut(s) 79
CpoI CGGWCCG 1 cut(s) 59
CseI GACGC 1 cut(s) 199
CspAI ACCGGT 1 cut(s) 56
CspI CGGWCCG 1 cut(s) 59
DpnI GATC 2 cut(s) 234, 284
DpnII GATC 2 cut(s) 232, 282
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 166
Eco24I GRGCYC 2 cut(s) 168, 256
Eco47I GGWCC 3 cut(s) 59, 72, 120
Eco53kI GAGCTC 1 cut(s) 166
Eco88I CYCGRG 2 cut(s) 79, 278
EcoICRI GAGCTC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 24, 133
EcoT38I GRGCYC 2 cut(s) 168, 256
FaiI YATR 1 cut(s) 21
FauI CCCGC 1 cut(s) 47
Fnu4HI GCNGC 3 cut(s) 43, 46, 294
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 2 cut(s) 168, 256
Fsp4HI GCNGC 3 cut(s) 43, 46, 294
GlaI GCGC 1 cut(s) 30
GluI GCNGC 3 cut(s) 43, 46, 294
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 4 cut(s) 84, 138, 216, 293
HapII CCGG 4 cut(s) 57, 67, 80, 309
HgaI GACGC 1 cut(s) 199
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 210
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HinfI GANTC 1 cut(s) 320
HpaII CCGG 4 cut(s) 57, 67, 80, 309
HphI GGTGA 4 cut(s) 172, 184, 229, 248
Hpy166II GTNNAC 1 cut(s) 72
Hpy188I TCNGA 5 cut(s) 35, 63, 120, 147, 325
Hpy188III TCNNGA 2 cut(s) 244, 280
Hpy8I GTNNAC 1 cut(s) 72
Hpy99I CGWCG 1 cut(s) 215
HpyCH4IV ACGT 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 210
HspAI GCGC 1 cut(s) 29
KspI CCGCGG 1 cut(s) 43
Kzo9I GATC 2 cut(s) 232, 282
LmnI GCTCC 3 cut(s) 36, 163, 171
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 178
MalI GATC 2 cut(s) 234, 284
MboI GATC 2 cut(s) 232, 282
MboII GAAGA 1 cut(s) 103
MhlI GDGCHC 2 cut(s) 168, 256
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 4 cut(s) 57, 67, 80, 309
MspR9I CCNGG 5 cut(s) 26, 80, 81, 135, 310
MvaI CCWGG 2 cut(s) 26, 135
MvnI CGCG 1 cut(s) 42
NciI CCSGG 3 cut(s) 80, 81, 310
NdeII GATC 2 cut(s) 232, 282
NlaIV GGNNCC 1 cut(s) 122
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 178
PaeR7I CTCGAG 1 cut(s) 278
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PfeI GAWTC 1 cut(s) 320
PinAI ACCGGT 1 cut(s) 56
PkrI GCNGC 3 cut(s) 44, 47, 295
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 24, 133
PspGI CCWGG 2 cut(s) 24, 133
PspN4I GGNNCC 1 cut(s) 122
PspPI GGNCC 4 cut(s) 59, 72, 82, 120
Rsr2I CGGWCCG 1 cut(s) 59
RsrII CGGWCCG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 168
SacII CCGCGG 1 cut(s) 43
SatI GCNGC 3 cut(s) 43, 46, 294
Sau3AI GATC 2 cut(s) 232, 282
Sau96I GGNCC 4 cut(s) 59, 72, 82, 120
ScrFI CCNGG 5 cut(s) 26, 80, 81, 135, 310
SduI GDGCHC 2 cut(s) 168, 256
SetI ASST 6 cut(s) 16, 111, 130, 168, 180, 188
Sfr274I CTCGAG 1 cut(s) 278
Sfr303I CCGCGG 1 cut(s) 43
SgrBI CCGCGG 1 cut(s) 43
SinI GGWCC 3 cut(s) 59, 72, 120
SlaI CTCGAG 1 cut(s) 278
SmaI CCCGGG 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SrfI GCCCGGGC 1 cut(s) 81
SsiI CCGC 4 cut(s) 40, 42, 45, 294
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 5 cut(s) 24, 78, 79, 133, 308
TaiI ACGT 1 cut(s) 180
TaqI TCGA 4 cut(s) 243, 270, 279, 285
TauI GCSGC 3 cut(s) 45, 48, 296
TfiI GAWTC 1 cut(s) 320
TseFI GTSAC 1 cut(s) 178
Tsp45I GTSAC 1 cut(s) 178
TspMI CCCGGG 1 cut(s) 79
VpaK11BI GGWCC 3 cut(s) 59, 72, 120
XagI CCTNNNNNAGG 1 cut(s) 132
XhoI CTCGAG 1 cut(s) 278
XmaI CCCGGG 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.