MD14G1064400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010227
Physical Location & Seq
Forward (+)
6801849 .. 6802175
327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1064400.v1.1.491

Sequence Viewer

Length: 327 bp
ATGCTCCGCCATCGCCTCGTCCAGGCGCTCCGAACCCGCGGCGGCTCTGCCACCGGTCCGAGCCGGTGGACCAGCCCGGGCCACGAGGAACAACCCAAAGGCTACCTCTTCAATCGGACCCCACCTCCCCCAGGCCAGTCTCGGAAATGGGAGGATTGGGAGCTCCCGTGCTACGTCACCAGCTTCCTCACCATCGTCATCCTCGGCGTCGGCCTCAACGCCAAGCCTGATCTCACCATCGAGACTTGGGCTCACCAGAAAGCCCTCGAACGGCTCGAGATCGAGCAGGCCGCCCTCTCTGTCGCCGGGTCGGGCGAATCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

109

Amino Acids

11.99

Weight (kDa)

6.83

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 39
AciI CCGC 5 cut(s) 7, 37, 39, 42, 291
AcyI GRCGYC 1 cut(s) 207
AfiI CCNNNNNNNGG 3 cut(s) 22, 131, 270
AgeI ACCGGT 1 cut(s) 53
AgsI TTSAA 1 cut(s) 112
AjnI CCWGG 2 cut(s) 21, 130
AluBI AGCT 2 cut(s) 163, 183
AluI AGCT 2 cut(s) 163, 183
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 2 cut(s) 144, 236
Ama87I CYCGRG 2 cut(s) 76, 275
AoxI GGCC 4 cut(s) 79, 133, 211, 288
AsiGI ACCGGT 1 cut(s) 53
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 4 cut(s) 56, 69, 79, 117
AsuC2I CCSGG 3 cut(s) 77, 78, 307
AsuHPI GGTGA 4 cut(s) 169, 181, 226, 245
AvaI CYCGRG 2 cut(s) 76, 275
AvaII GGWCC 3 cut(s) 56, 69, 117
BanII GRGCYC 2 cut(s) 165, 253
BauI CACGAG 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 165
BccI CCATC 3 cut(s) 18, 200, 245
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 2 cut(s) 23, 132
BcnI CCSGG 3 cut(s) 77, 78, 307
BcoDI GTCTC 2 cut(s) 144, 236
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 3 cut(s) 40, 43, 291
BlsI GCNGC 3 cut(s) 41, 44, 292
Bme1390I CCNGG 5 cut(s) 23, 77, 78, 132, 307
Bme18I GGWCC 3 cut(s) 56, 69, 117
BmeT110I CYCGRG 2 cut(s) 76, 275
BmgT120I GGNCC 4 cut(s) 56, 69, 79, 117
BmiI GGNNCC 1 cut(s) 119
BmrFI CCNGG 5 cut(s) 23, 77, 78, 132, 307
BpuMI CCSGG 3 cut(s) 77, 78, 307
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 4 cut(s) 37, 76, 130, 202
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 152, 182
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 131, 270
Bse118I RCCGGY 2 cut(s) 53, 63
Bse1I ACTGG 1 cut(s) 136
BseBI CCWGG 2 cut(s) 23, 132
BseDI CCNNGG 4 cut(s) 37, 76, 130, 202
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 22, 131, 270
BseNI ACTGG 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 4 cut(s) 81, 135, 213, 290
BshTI ACCGGT 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 2 cut(s) 76, 275
BsiSI CCGG 4 cut(s) 54, 64, 77, 306
BslI CCNNNNNNNGG 3 cut(s) 22, 131, 270
BsmAI GTCTC 2 cut(s) 144, 236
BsnI GGCC 4 cut(s) 81, 135, 213, 290
BsoBI CYCGRG 2 cut(s) 76, 275
Bsp1286I GDGCHC 2 cut(s) 165, 253
Bsp143I GATC 2 cut(s) 229, 279
BspACI CCGC 5 cut(s) 7, 37, 39, 42, 291
BspANI GGCC 4 cut(s) 81, 135, 213, 290
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 119
BsrFI RCCGGY 2 cut(s) 53, 63
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 2 cut(s) 53, 63
BssECI CCNNGG 4 cut(s) 37, 76, 130, 202
BssMI GATC 2 cut(s) 229, 279
BssNI GRCGYC 1 cut(s) 207
BssSI CACGAG 1 cut(s) 83
Bst2BI CACGAG 1 cut(s) 83
Bst2UI CCWGG 2 cut(s) 23, 132
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 37
BstENI CCTNNNNNAGG 2 cut(s) 20, 129
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 2 cut(s) 232, 282
BstMAI GTCTC 2 cut(s) 144, 236
BstMBI GATC 2 cut(s) 229, 279
BstNI CCWGG 2 cut(s) 23, 132
BstSCI CCNGG 5 cut(s) 21, 75, 76, 130, 305
BstUI CGCG 1 cut(s) 39
BsuRI GGCC 4 cut(s) 81, 135, 213, 290
BtgI CCRYGG 1 cut(s) 37
BtsCI GGATG 1 cut(s) 198
Cac8I GCNNGC 1 cut(s) 288
CfoI GCGC 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 53, 63
Cfr13I GGNCC 4 cut(s) 56, 69, 79, 117
Cfr42I CCGCGG 1 cut(s) 40
Cfr9I CCCGGG 1 cut(s) 76
CpoI CGGWCCG 1 cut(s) 56
CseI GACGC 1 cut(s) 196
CspAI ACCGGT 1 cut(s) 53
CspI CGGWCCG 1 cut(s) 56
DpnI GATC 2 cut(s) 231, 281
DpnII GATC 2 cut(s) 229, 279
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Ecl136II GAGCTC 1 cut(s) 163
Eco24I GRGCYC 2 cut(s) 165, 253
Eco47I GGWCC 3 cut(s) 56, 69, 117
Eco53kI GAGCTC 1 cut(s) 163
Eco88I CYCGRG 2 cut(s) 76, 275
EcoICRI GAGCTC 1 cut(s) 163
EcoNI CCTNNNNNAGG 2 cut(s) 20, 129
EcoRII CCWGG 2 cut(s) 21, 130
EcoT38I GRGCYC 2 cut(s) 165, 253
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 3 cut(s) 40, 43, 291
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 2 cut(s) 165, 253
Fsp4HI GCNGC 3 cut(s) 40, 43, 291
GlaI GCGC 1 cut(s) 27
GluI GCNGC 3 cut(s) 40, 43, 291
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 4 cut(s) 81, 135, 213, 290
HapII CCGG 4 cut(s) 54, 64, 77, 306
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 28
Hin1I GRCGYC 1 cut(s) 207
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HinfI GANTC 1 cut(s) 317
HpaII CCGG 4 cut(s) 54, 64, 77, 306
HphI GGTGA 4 cut(s) 169, 181, 226, 245
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 5 cut(s) 32, 60, 117, 144, 322
Hpy188III TCNNGA 2 cut(s) 241, 277
Hpy8I GTNNAC 1 cut(s) 69
Hpy99I CGWCG 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 207
HspAI GCGC 1 cut(s) 26
KspI CCGCGG 1 cut(s) 40
Kzo9I GATC 2 cut(s) 229, 279
LmnI GCTCC 4 cut(s) 9, 33, 160, 168
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 175
MalI GATC 2 cut(s) 231, 281
MboI GATC 2 cut(s) 229, 279
MboII GAAGA 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 165, 253
MspA1I CMGCKG 1 cut(s) 39
MspI CCGG 4 cut(s) 54, 64, 77, 306
MspR9I CCNGG 5 cut(s) 23, 77, 78, 132, 307
MvaI CCWGG 2 cut(s) 23, 132
MvnI CGCG 1 cut(s) 39
NciI CCSGG 3 cut(s) 77, 78, 307
NdeII GATC 2 cut(s) 229, 279
NlaIV GGNNCC 1 cut(s) 119
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 175
PaeR7I CTCGAG 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 1 cut(s) 317
PinAI ACCGGT 1 cut(s) 53
PkrI GCNGC 3 cut(s) 41, 44, 292
Psp124BI GAGCTC 1 cut(s) 165
Psp6I CCWGG 2 cut(s) 21, 130
PspGI CCWGG 2 cut(s) 21, 130
PspN4I GGNNCC 1 cut(s) 119
PspPI GGNCC 4 cut(s) 56, 69, 79, 117
Rsr2I CGGWCCG 1 cut(s) 56
RsrII CGGWCCG 1 cut(s) 56
SacI GAGCTC 1 cut(s) 165
SacII CCGCGG 1 cut(s) 40
SatI GCNGC 3 cut(s) 40, 43, 291
Sau3AI GATC 2 cut(s) 229, 279
Sau96I GGNCC 4 cut(s) 56, 69, 79, 117
ScrFI CCNGG 5 cut(s) 23, 77, 78, 132, 307
SduI GDGCHC 2 cut(s) 165, 253
SetI ASST 5 cut(s) 108, 127, 165, 177, 185
Sfr274I CTCGAG 1 cut(s) 275
Sfr303I CCGCGG 1 cut(s) 40
SgrBI CCGCGG 1 cut(s) 40
SinI GGWCC 3 cut(s) 56, 69, 117
SlaI CTCGAG 1 cut(s) 275
SmaI CCCGGG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SrfI GCCCGGGC 1 cut(s) 78
SsiI CCGC 5 cut(s) 7, 37, 39, 42, 291
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 5 cut(s) 21, 75, 76, 130, 305
TaiI ACGT 1 cut(s) 177
TaqI TCGA 4 cut(s) 240, 267, 276, 282
TauI GCSGC 3 cut(s) 42, 45, 293
TfiI GAWTC 1 cut(s) 317
TseFI GTSAC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 175
TspMI CCCGGG 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 56, 69, 117
XagI CCTNNNNNAGG 2 cut(s) 20, 129
XhoI CTCGAG 1 cut(s) 275
XmaI CCCGGG 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.