MD07G1006600.v1.1

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013498
Physical Location & Seq
Forward (+)
629780 .. 633367
3588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1006600.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGACACAACGACGACAACAACAGCAAGCGGCTTGAAAACCCTAACACCTTTCTCCCCCTTCTCTCCTTTATCTCCTTTCCGCATGTGCTCCTCAAAAGTTCCTCTCTTTCGTTCTCTCTGCTCGGCCCATCTCCACCGCAGCTCCCGCCTCTCTCTGTCCTCCGCTCGTTTCTCTCCCCTCTGCTCTCTCTCTGCAGCTGATGGGATTGGGAGCAGAGGAGAGTGCTTTCTGGCAAATGGGGTAGGGGAGGCAGTGAAGGAAGAACAAGTTTCACAAAGGAAGATTCTTCAAGTGGTTTTAGTCTCTCCTCAGATTCCTGGAAATACAGGTTGCATTGCCAGAACATGTGCAGCATCAGCTGTTGGGCTTCATCTTGTTGGGCCACTAGGATTCAAGATTGATGATGCAAAATTAAAGCGTGCTGGATTGGACTATTGGCCATATGTGGTGGTTAAAGTTCACAGCTCATGGGCAGAATTTCAAGACTATTTCAGGCGACAGGAGGGGGAAAAGCGGTTGCTTGCATTTACAAAGAGAGGAACAAAATTTCATTCAGACTTTTCTTATAGAAGGGGTGATTTTCTCATATTTGGCTCCGAAACATCCGGCCTACCACCTGAAGCGCTCGAAGACTGCAAAAGTGAAACGATTGGTGGTGGGACAATTAGGATTCCAATGGTTGAAACTTATGTAAGATGTCTGAATCTCTCTGTAAGTGTTGGCATTGCTCTGTATGAAGCTGCCAGACAGCTAAATTACGAGCAGCTTGATTTTCCAAATGAAAACTGTATTGATAGTGAAAAATCATTTGTCACCGAGGACATTTTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

279

Amino Acids

30.51

Weight (kDa)

8.05

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 97 - 246 4.6e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26880
fragaria_vesca FvH4_7g00690 FvH4_7g00690 FvH4_7g00690
malus_domestica MD07G1006600.v1.1
prunus_persica Prupe.2G009700_v2.0.a1 Prupe.2G009700_v2.0.a1
pyrus_communis pycom07g00530
rosa_chinensis RchiOBHm_Chr1g0314911
rosa_laevigata RLG00000030755
rosa_roxburghii Rroxscaffold_4G00331680
rosa_rugosa Rorug01G0002900 Rorug01G0003000
rosa_samantha Rh1AG013900 Rh1BG005500 Rh1CG012200 Rh1DG007300
rosa_wichuraiana Rw1G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 167
AciI CCGC 6 cut(s) 30, 82, 139, 148, 165, 517
AcoI YGGCCR 1 cut(s) 440
AcsI RAATTY 2 cut(s) 479, 548
AcuI CTGAAG 1 cut(s) 642
AfeI AGCGCT 1 cut(s) 627
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 5 cut(s) 37, 293, 397, 485, 686
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 7 cut(s) 144, 200, 362, 468, 743, 754, 769
AluI AGCT 7 cut(s) 144, 200, 362, 468, 743, 754, 769
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 310
Aor51HI AGCGCT 1 cut(s) 627
AoxI GGCC 4 cut(s) 126, 383, 440, 610
ApeKI GCWGC 5 cut(s) 141, 197, 353, 743, 766
ApoI RAATTY 2 cut(s) 479, 548
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 2 cut(s) 127, 383
AsuHPI GGTGA 2 cut(s) 590, 808
BalI TGGCCA 1 cut(s) 442
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 5 cut(s) 153, 209, 365, 730, 778
BccI CCATC 2 cut(s) 138, 197
BciT130I CCWGG 1 cut(s) 321
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 629
BisI GCNGC 6 cut(s) 31, 142, 198, 354, 744, 767
BlsI GCNGC 6 cut(s) 32, 143, 199, 355, 745, 768
Bme1390I CCNGG 1 cut(s) 321
BmgT120I GGNCC 2 cut(s) 127, 383
BmiI GGNNCC 1 cut(s) 598
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 365, 397
BpiI GAAGAC 1 cut(s) 639
BsaJI CCNNGG 1 cut(s) 819
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bse3DI GCAATG 2 cut(s) 336, 726
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 819
BseGI GGATG 1 cut(s) 605
BseMI GCAATG 2 cut(s) 336, 726
BseMII CTCAG 1 cut(s) 326
BseRI GAGGAG 3 cut(s) 82, 234, 300
BseXI GCAGC 5 cut(s) 153, 209, 365, 730, 778
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 4 cut(s) 128, 385, 442, 612
BsiHKAI GWGCWC 1 cut(s) 92
BsiSI CCGG 1 cut(s) 609
BslFI GGGAC 1 cut(s) 676
BsmAI GTCTC 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 676
BsnI GGCC 4 cut(s) 128, 385, 442, 612
Bsp1286I GDGCHC 1 cut(s) 92
BspACI CCGC 6 cut(s) 30, 82, 139, 148, 165, 517
BspANI GGCC 4 cut(s) 128, 385, 442, 612
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 598
BspMAI CTGCAG 1 cut(s) 199
BsrBI CCGCTC 1 cut(s) 167
BsrDI GCAATG 2 cut(s) 336, 726
BssECI CCNNGG 1 cut(s) 819
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 791
BstC8I GCNNGC 3 cut(s) 28, 423, 525
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 605
BstH2I RGCGCY 1 cut(s) 629
BstHHI GCGC 1 cut(s) 628
BstMAI GTCTC 1 cut(s) 310
BstMWI GCNNNNNNNGC 2 cut(s) 147, 359
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 2 cut(s) 88, 351
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 5 cut(s) 153, 209, 365, 730, 778
BstV2I GAAGAC 1 cut(s) 639
BsuRI GGCC 4 cut(s) 128, 385, 442, 612
BtsCI GGATG 1 cut(s) 605
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 261
Cac8I GCNNGC 3 cut(s) 28, 423, 525
CfoI GCGC 1 cut(s) 628
Cfr13I GGNCC 2 cut(s) 127, 383
CviAII CATG 3 cut(s) 85, 348, 471
DdeI CTNAG 1 cut(s) 312
EaeI YGGCCR 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 627
Eco57I CTGAAG 1 cut(s) 642
EcoRII CCWGG 1 cut(s) 319
FaeI CATG 3 cut(s) 88, 351, 474
FaiI YATR 9 cut(s) 86, 349, 445, 447, 472, 570, 590, 693, 738
FaqI GGGAC 1 cut(s) 676
FatI CATG 3 cut(s) 84, 347, 470
FauI CCCGC 1 cut(s) 155
FauNDI CATATG 1 cut(s) 445
Fnu4HI GCNGC 6 cut(s) 31, 142, 198, 354, 744, 767
FokI GGATG 1 cut(s) 592
Fsp4HI GCNGC 6 cut(s) 31, 142, 198, 354, 744, 767
FspBI CTAG 1 cut(s) 389
GlaI GCGC 1 cut(s) 627
GluI GCNGC 6 cut(s) 31, 142, 198, 354, 744, 767
HaeII RGCGCY 1 cut(s) 629
HaeIII GGCC 4 cut(s) 128, 385, 442, 612
HapII CCGG 1 cut(s) 609
HhaI GCGC 1 cut(s) 628
Hin1II CATG 3 cut(s) 88, 351, 474
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HinfI GANTC 5 cut(s) 286, 316, 393, 673, 706
HpaII CCGG 1 cut(s) 609
HphI GGTGA 2 cut(s) 590, 808
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 4 cut(s) 315, 559, 601, 705
Hpy188III TCNNGA 2 cut(s) 397, 485
Hpy8I GTNNAC 1 cut(s) 463
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 3 cut(s) 70, 253, 567
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4V TGCA 6 cut(s) 197, 336, 353, 410, 527, 639
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 359
HpyF3I CTNAG 1 cut(s) 312
Hsp92II CATG 3 cut(s) 88, 351, 474
HspAI GCGC 1 cut(s) 626
LmnI GCTCC 4 cut(s) 95, 149, 213, 602
Lsp1109I GCAGC 5 cut(s) 153, 209, 365, 730, 778
LweI GCATC 2 cut(s) 365, 397
MaeI CTAG 1 cut(s) 389
MaeIII GTNAC 1 cut(s) 814
MbiI CCGCTC 1 cut(s) 167
MboII GAAGA 4 cut(s) 275, 281, 295, 644
MhlI GDGCHC 1 cut(s) 92
MlsI TGGCCA 1 cut(s) 442
MluCI AATT 5 cut(s) 413, 479, 548, 666, 757
MluNI TGGCCA 1 cut(s) 442
Mox20I TGGCCA 1 cut(s) 442
MscI TGGCCA 1 cut(s) 442
MseI TTAA 3 cut(s) 416, 456, 835
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 2 cut(s) 200, 362
MspI CCGG 1 cut(s) 609
MspR9I CCNGG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 2 cut(s) 147, 359
NdeI CATATG 1 cut(s) 445
NlaIII CATG 3 cut(s) 88, 351, 474
NlaIV GGNNCC 1 cut(s) 598
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 1 cut(s) 814
NspI RCATGY 2 cut(s) 88, 351
PciI ACATGT 1 cut(s) 347
PfeI GAWTC 5 cut(s) 286, 316, 393, 673, 706
PfoI TCCNGGA 1 cut(s) 319
PkrI GCNGC 6 cut(s) 32, 143, 199, 355, 745, 768
PscI ACATGT 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 598
PspPI GGNCC 2 cut(s) 127, 383
PstI CTGCAG 1 cut(s) 199
PvuII CAGCTG 2 cut(s) 200, 362
SaqAI TTAA 3 cut(s) 416, 456, 835
SatI GCNGC 6 cut(s) 31, 142, 198, 354, 744, 767
Sau96I GGNCC 2 cut(s) 127, 383
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 1 cut(s) 92
SfaNI GCATC 2 cut(s) 365, 397
SfcI CTRYAG 1 cut(s) 195
Sse9I AATT 5 cut(s) 413, 479, 548, 666, 757
SsiI CCGC 6 cut(s) 30, 82, 139, 148, 165, 517
SspMI CTAG 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 319
TaaI ACNGT 1 cut(s) 791
TaqI TCGA 1 cut(s) 630
TasI AATT 5 cut(s) 413, 479, 548, 666, 757
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 5 cut(s) 286, 316, 393, 673, 706
Tru1I TTAA 3 cut(s) 416, 456, 835
Tru9I TTAA 3 cut(s) 416, 456, 835
TscAI CASTG 1 cut(s) 261
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 5 cut(s) 141, 197, 353, 743, 766
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 4 cut(s) 362, 542, 753, 798
TspRI CASTG 1 cut(s) 261
XapI RAATTY 2 cut(s) 479, 548
XceI RCATGY 2 cut(s) 88, 351
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.