Rh1CG012200

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0013498
Physical Location & Seq
Forward (+)
1887041 .. 1892098
5058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG012200.1

Sequence Viewer

Length: 585 bp
ATGCTGCCGGCAATGGACAGCAGCGGCTTGAAAACCCTAACCCCTTTCTCTCCTTTAAATCCATTTCGCTCCAGAGGAGGAGCTCCTCTCTTTGCTTCTCTATACTTGGCTCATCTCAACCGCAAATCCCGCCTCTCTCTTTCCTCCACTCGCTGCTCACTCTCTGAGAGGGGCAAAGAGTGTTCTTTGGCTCATGGGGCAGGGGAGGCACTCAAACAAGTCTCACAAACTAAGATTCTTCAAGTCGTTCTCGTGTCTCCTCAGATTCCTGGAAATACAGGTTGCATTGCCAGAACATGTGCAGCATCAGCAGTTGGGCTTCATCTGGTTGGGCCGTTGGGCTTTCAGATTGATGATGCAAAATTAAAGCGTGCTGGATTGGACTATTGGCCATATGTGGTTGTCAAAGTTCACAGCTCATGGGCAGAGTTTCAAGACTATTTCAGGCAACAGGATGGTGAAAAGCGGTTGCTTGCATTTACAAAGAGAGCGACAAAATTTCATTCAGTAAGTTCCTGTTGTTTTAGTCTCTCCATTAGATTTTACAGTAATGAAATCTGTGAAGATACCCTTTGTACAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

194

Amino Acids

21.28

Weight (kDa)

9.3

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 80 - 170 4.3e-15 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26880
fragaria_vesca FvH4_7g00690 FvH4_7g00690 FvH4_7g00690
malus_domestica MD07G1006600.v1.1
prunus_persica Prupe.2G009700_v2.0.a1 Prupe.2G009700_v2.0.a1
pyrus_communis pycom07g00530
rosa_chinensis RchiOBHm_Chr1g0314911
rosa_laevigata RLG00000030755
rosa_roxburghii Rroxscaffold_4G00331680
rosa_rugosa Rorug01G0002900 Rorug01G0003000
rosa_samantha Rh1AG013900 Rh1BG005500 Rh1CG012200 Rh1DG007300
rosa_wichuraiana Rw1G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 24, 121, 130, 466
AcoI YGGCCR 1 cut(s) 389
AcsI RAATTY 1 cut(s) 497
AfaI GTAC 1 cut(s) 577
AflIII ACRYGT 1 cut(s) 296
AgsI TTSAA 3 cut(s) 31, 242, 434
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 2 cut(s) 83, 417
AluI AGCT 2 cut(s) 83, 417
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 3 cut(s) 226, 261, 533
AoxI GGCC 2 cut(s) 332, 389
ApeKI GCWGC 4 cut(s) 4, 21, 153, 302
ApoI RAATTY 1 cut(s) 497
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 1 cut(s) 470
BalI TGGCCA 1 cut(s) 391
BanII GRGCYC 1 cut(s) 85
BauI CACGAG 1 cut(s) 251
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 3 cut(s) 33, 140, 314
BccI CCATC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 3 cut(s) 226, 261, 533
BisI GCNGC 5 cut(s) 5, 22, 25, 154, 303
BlsI GCNGC 5 cut(s) 6, 23, 26, 155, 304
Bme1390I CCNGG 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 332
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 2 cut(s) 314, 346
BplI GAGNNNNNCTC 2 cut(s) 72, 104
BpmI CTGGAG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 7
Bse3DI GCAATG 2 cut(s) 18, 285
BseBI CCWGG 1 cut(s) 270
BseGI GGATG 1 cut(s) 460
BseMI GCAATG 2 cut(s) 18, 285
BseMII CTCAG 2 cut(s) 156, 275
BseRI GAGGAG 4 cut(s) 75, 90, 93, 249
BseXI GCAGC 3 cut(s) 33, 140, 314
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 2 cut(s) 334, 391
BsiHKAI GWGCWC 1 cut(s) 85
BsiSI CCGG 1 cut(s) 8
BsmAI GTCTC 3 cut(s) 226, 261, 533
BsnI GGCC 2 cut(s) 334, 391
Bsp1286I GDGCHC 1 cut(s) 85
Bsp1407I TGTACA 1 cut(s) 575
BspACI CCGC 4 cut(s) 24, 121, 130, 466
BspANI GGCC 2 cut(s) 334, 391
BspCNI CTCAG 2 cut(s) 157, 274
BsrDI GCAATG 2 cut(s) 18, 285
BsrFI RCCGGY 1 cut(s) 7
BsrGI TGTACA 1 cut(s) 575
BssAI RCCGGY 1 cut(s) 7
BssSI CACGAG 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 548
BstAUI TGTACA 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 9, 372, 474
BstDEI CTNAG 3 cut(s) 165, 231, 261
BstF5I GGATG 1 cut(s) 460
BstMAI GTCTC 3 cut(s) 226, 261, 533
BstMWI GCNNNNNNNGC 4 cut(s) 129, 197, 206, 308
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 268
BstV1I GCAGC 3 cut(s) 33, 140, 314
BsuRI GGCC 2 cut(s) 334, 391
BtsCI GGATG 1 cut(s) 460
Cac8I GCNNGC 3 cut(s) 9, 372, 474
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 576
CviAII CATG 3 cut(s) 194, 297, 420
CviJI RGCY 9 cut(s) 27, 83, 110, 191, 319, 334, 342, 391, 417
CviKI_1 RGCY 9 cut(s) 27, 83, 110, 191, 319, 334, 342, 391, 417
CviQI GTAC 1 cut(s) 576
DdeI CTNAG 3 cut(s) 165, 231, 261
DraI TTTAAA 1 cut(s) 57
EaeI YGGCCR 1 cut(s) 389
Ecl136II GAGCTC 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 85
FaeI CATG 3 cut(s) 197, 300, 423
FaiI YATR 6 cut(s) 103, 195, 298, 394, 396, 421
FalI AAGNNNNNCTT 1 cut(s) 555
FatI CATG 3 cut(s) 193, 296, 419
FauI CCCGC 1 cut(s) 137
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 5 cut(s) 5, 22, 25, 154, 303
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 5 cut(s) 5, 22, 25, 154, 303
GluI GCNGC 5 cut(s) 5, 22, 25, 154, 303
GsuI CTGGAG 1 cut(s) 55
HaeIII GGCC 2 cut(s) 334, 391
HapII CCGG 1 cut(s) 8
Hin1II CATG 3 cut(s) 197, 300, 423
HinfI GANTC 2 cut(s) 235, 265
HpaII CCGG 1 cut(s) 8
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 3 cut(s) 166, 264, 348
Hpy188III TCNNGA 2 cut(s) 72, 434
Hpy8I GTNNAC 1 cut(s) 412
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4IV ACGT 1 cut(s) 581
HpyCH4V TGCA 4 cut(s) 285, 302, 359, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 197, 206, 308
HpyF3I CTNAG 3 cut(s) 165, 231, 261
HpySE526I ACGT 1 cut(s) 581
Hsp92II CATG 3 cut(s) 197, 300, 423
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
LmnI GCTCC 3 cut(s) 74, 80, 88
Lsp1109I GCAGC 3 cut(s) 33, 140, 314
LweI GCATC 2 cut(s) 314, 346
MaeII ACGT 1 cut(s) 581
MboII GAAGA 2 cut(s) 230, 575
MhlI GDGCHC 1 cut(s) 85
MlsI TGGCCA 1 cut(s) 391
MluCI AATT 2 cut(s) 362, 497
MluNI TGGCCA 1 cut(s) 391
MnlI CCTC 8 cut(s) 68, 71, 96, 143, 154, 162, 199, 270
Mox20I TGGCCA 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 7
MscI TGGCCA 1 cut(s) 391
MseI TTAA 2 cut(s) 56, 365
Msp20I TGGCCA 1 cut(s) 391
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 4 cut(s) 129, 197, 206, 308
NaeI GCCGGC 1 cut(s) 9
NdeI CATATG 1 cut(s) 394
NgoMIV GCCGGC 1 cut(s) 7
NlaIII CATG 3 cut(s) 197, 300, 423
NspI RCATGY 1 cut(s) 300
PciI ACATGT 1 cut(s) 296
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 2 cut(s) 235, 265
PfoI TCCNGGA 1 cut(s) 268
PkrI GCNGC 5 cut(s) 6, 23, 26, 155, 304
PscI ACATGT 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspPI GGNCC 1 cut(s) 332
RsaI GTAC 1 cut(s) 577
RsaNI GTAC 1 cut(s) 576
SacI GAGCTC 1 cut(s) 85
SaqAI TTAA 2 cut(s) 56, 365
SatI GCNGC 5 cut(s) 5, 22, 25, 154, 303
Sau96I GGNCC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 85
SetI ASST 4 cut(s) 85, 283, 419, 584
SfaNI GCATC 2 cut(s) 314, 346
Sse9I AATT 2 cut(s) 362, 497
SsiI CCGC 4 cut(s) 24, 121, 130, 466
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 268
TaaI ACNGT 1 cut(s) 548
TaiI ACGT 1 cut(s) 584
TasI AATT 2 cut(s) 362, 497
TatI WGTACW 1 cut(s) 575
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 2 cut(s) 235, 265
Tru1I TTAA 2 cut(s) 56, 365
Tru9I TTAA 2 cut(s) 56, 365
TseI GCWGC 4 cut(s) 4, 21, 153, 302
TspDTI ATGAA 3 cut(s) 311, 491, 567
XapI RAATTY 1 cut(s) 497
XceI RCATGY 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.