Rh1BG005500

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0013498
Physical Location & Seq
Forward (+)
750432 .. 753422
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG005500.1

Sequence Viewer

Length: 786 bp
ATGCTGCCGGCAATGGACAGCAGCGGCTTGAAAACCCTAACCCCTTTCTCTCCTTTAAATCCATTTCGCTCCAGAGGAGGAGCTCCTCTCTTTGCTTCTCTATACTTGGCTCATCTCAACCGCAAATCCCGCCTCTCTCTTTCCTCCACTCGCTGCTCACTCTCTGAGAGGGGCAAAGAGTGTTCTTTGGCTCATGGGGCAGGGGAGGCACTCAAACAAGTCTCACAAACTAAGATTCTTCAAGTCGTTCTCGTGTCTCCTCAGATTCCTGGAAATACAGGTTGCATTGCCAGAACATGTGCAGCATCAGCAGTTGGGCTTCATCTGGTTGGGCCGTTGGGCTTTCAGATTGATGATGCAAAATTAAAGCGTGCTGGATTGGACTATTGGCCATATGTGGTTGTCAAAGTTCACAGCTCATGGGCAGAGTTTCAAGACTATTTCAGGCAACAGGATGGTGAAAAGCGGTTGCTTGCATTTACAAAGAGAGCGACAAAATTTCATTCAGATTTTTCTTATAGAAGAGGTGATTTTCTCATATTTGGGTCAGAAACAAGCGGCCTACCACCTGAAGCCCTCCAAGACTGCAAGAGTGGAACATTTGGCGGTGGAACACTTAGGATTCCAATGGTTGAAACGTATGTAAGATGTCTCAATCTCTCTGTAAGTGTTGGCATTGCTTTATATGAAGCTTCGAGACAGTTAAATTATGAGCAGCTTCAATTTCCACTTGAAAATTGTATTGATAGTGAAAAATCAATTGTCACAGAGGACATTTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

261

Amino Acids

28.68

Weight (kDa)

8.81

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 80 - 229 8.4e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26880
fragaria_vesca FvH4_7g00690 FvH4_7g00690 FvH4_7g00690
malus_domestica MD07G1006600.v1.1
prunus_persica Prupe.2G009700_v2.0.a1 Prupe.2G009700_v2.0.a1
pyrus_communis pycom07g00530
rosa_chinensis RchiOBHm_Chr1g0314911
rosa_laevigata RLG00000030755
rosa_roxburghii Rroxscaffold_4G00331680
rosa_rugosa Rorug01G0002900 Rorug01G0003000
rosa_samantha Rh1AG013900 Rh1BG005500 Rh1CG012200 Rh1DG007300
rosa_wichuraiana Rw1G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 24, 121, 130, 466, 558, 606
AcoI YGGCCR 1 cut(s) 389
AcsI RAATTY 1 cut(s) 497
AcuI CTGAAG 1 cut(s) 591
AflIII ACRYGT 1 cut(s) 296
AgsI TTSAA 6 cut(s) 31, 242, 434, 635, 722, 734
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 4 cut(s) 83, 417, 692, 718
AluI AGCT 4 cut(s) 83, 417, 692, 718
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 4 cut(s) 226, 261, 656, 691
AoxI GGCC 3 cut(s) 332, 389, 559
ApeKI GCWGC 5 cut(s) 4, 21, 153, 302, 715
ApoI RAATTY 1 cut(s) 497
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 470, 539
BalI TGGCCA 1 cut(s) 391
BanII GRGCYC 1 cut(s) 85
BauI CACGAG 1 cut(s) 251
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 4 cut(s) 33, 140, 314, 727
BccI CCATC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 4 cut(s) 226, 261, 656, 691
BisI GCNGC 7 cut(s) 5, 22, 25, 154, 303, 559, 716
BlsI GCNGC 7 cut(s) 6, 23, 26, 155, 304, 560, 717
Bme1390I CCNGG 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 332
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 2 cut(s) 314, 346
BplI GAGNNNNNCTC 2 cut(s) 72, 104
BpmI CTGGAG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 7
Bse3DI GCAATG 3 cut(s) 18, 285, 675
BseBI CCWGG 1 cut(s) 270
BseGI GGATG 1 cut(s) 460
BseMI GCAATG 3 cut(s) 18, 285, 675
BseMII CTCAG 2 cut(s) 156, 275
BseRI GAGGAG 4 cut(s) 75, 90, 93, 249
BseXI GCAGC 4 cut(s) 33, 140, 314, 727
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 3 cut(s) 334, 391, 561
BsiHKAI GWGCWC 1 cut(s) 85
BsiSI CCGG 1 cut(s) 8
BsmAI GTCTC 4 cut(s) 226, 261, 656, 691
BsnI GGCC 3 cut(s) 334, 391, 561
Bsp1286I GDGCHC 1 cut(s) 85
BspACI CCGC 6 cut(s) 24, 121, 130, 466, 558, 606
BspANI GGCC 3 cut(s) 334, 391, 561
BspCNI CTCAG 2 cut(s) 157, 274
BsrDI GCAATG 3 cut(s) 18, 285, 675
BsrFI RCCGGY 1 cut(s) 7
BssAI RCCGGY 1 cut(s) 7
BssSI CACGAG 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 702
Bst6I CTCTTC 1 cut(s) 517
BstC8I GCNNGC 3 cut(s) 9, 372, 474
BstDEI CTNAG 4 cut(s) 165, 231, 261, 617
BstF5I GGATG 1 cut(s) 460
BstMAI GTCTC 4 cut(s) 226, 261, 656, 691
BstMWI GCNNNNNNNGC 4 cut(s) 129, 197, 206, 308
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 268
BstV1I GCAGC 4 cut(s) 33, 140, 314, 727
BsuRI GGCC 3 cut(s) 334, 391, 561
BtsCI GGATG 1 cut(s) 460
Cac8I GCNNGC 3 cut(s) 9, 372, 474
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 332
CviAII CATG 3 cut(s) 194, 297, 420
DdeI CTNAG 4 cut(s) 165, 231, 261, 617
DraI TTTAAA 1 cut(s) 57
EaeI YGGCCR 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 517
EarI CTCTTC 1 cut(s) 517
Ecl136II GAGCTC 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 591
EcoICRI GAGCTC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 85
FaeI CATG 3 cut(s) 197, 300, 423
FatI CATG 3 cut(s) 193, 296, 419
FauI CCCGC 1 cut(s) 137
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 7 cut(s) 5, 22, 25, 154, 303, 559, 716
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 7 cut(s) 5, 22, 25, 154, 303, 559, 716
GluI GCNGC 7 cut(s) 5, 22, 25, 154, 303, 559, 716
GsuI CTGGAG 1 cut(s) 55
HaeIII GGCC 3 cut(s) 334, 391, 561
HapII CCGG 1 cut(s) 8
Hin1II CATG 3 cut(s) 197, 300, 423
HindIII AAGCTT 1 cut(s) 690
HinfI GANTC 3 cut(s) 235, 265, 622
HpaII CCGG 1 cut(s) 8
HphI GGTGA 2 cut(s) 470, 539
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 5 cut(s) 166, 264, 348, 508, 550
Hpy188III TCNNGA 3 cut(s) 72, 434, 696
Hpy8I GTNNAC 1 cut(s) 412
HpyCH4III ACNGT 1 cut(s) 702
HpyCH4IV ACGT 1 cut(s) 638
HpyCH4V TGCA 6 cut(s) 285, 302, 359, 476, 588, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 197, 206, 308
HpyF3I CTNAG 4 cut(s) 165, 231, 261, 617
HpySE526I ACGT 1 cut(s) 638
Hsp92II CATG 3 cut(s) 197, 300, 423
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
LmnI GCTCC 3 cut(s) 74, 80, 88
Lsp1109I GCAGC 4 cut(s) 33, 140, 314, 727
LweI GCATC 2 cut(s) 314, 346
MaeII ACGT 1 cut(s) 638
MaeIII GTNAC 1 cut(s) 763
MboII GAAGA 2 cut(s) 230, 534
MfeI CAATTG 1 cut(s) 759
MhlI GDGCHC 1 cut(s) 85
MlsI TGGCCA 1 cut(s) 391
MluCI AATT 6 cut(s) 362, 497, 706, 722, 736, 759
MluNI TGGCCA 1 cut(s) 391
Mox20I TGGCCA 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 7
MscI TGGCCA 1 cut(s) 391
MseI TTAA 3 cut(s) 56, 365, 704
Msp20I TGGCCA 1 cut(s) 391
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 270
MunI CAATTG 1 cut(s) 759
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 4 cut(s) 129, 197, 206, 308
NaeI GCCGGC 1 cut(s) 9
NdeI CATATG 1 cut(s) 394
NgoMIV GCCGGC 1 cut(s) 7
NlaIII CATG 3 cut(s) 197, 300, 423
NmuCI GTSAC 1 cut(s) 763
NspI RCATGY 1 cut(s) 300
PciI ACATGT 1 cut(s) 296
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 235, 265, 622
PfoI TCCNGGA 1 cut(s) 268
PkrI GCNGC 7 cut(s) 6, 23, 26, 155, 304, 560, 717
PscI ACATGT 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspPI GGNCC 1 cut(s) 332
SacI GAGCTC 1 cut(s) 85
SaqAI TTAA 3 cut(s) 56, 365, 704
SatI GCNGC 7 cut(s) 5, 22, 25, 154, 303, 559, 716
Sau96I GGNCC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 85
SetI ASST 8 cut(s) 85, 283, 419, 529, 571, 641, 694, 720
SfaNI GCATC 2 cut(s) 314, 346
Sse9I AATT 6 cut(s) 362, 497, 706, 722, 736, 759
SsiI CCGC 6 cut(s) 24, 121, 130, 466, 558, 606
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 268
TaaI ACNGT 1 cut(s) 702
TaiI ACGT 1 cut(s) 641
TaqI TCGA 1 cut(s) 695
TasI AATT 6 cut(s) 362, 497, 706, 722, 736, 759
TauI GCSGC 2 cut(s) 27, 561
TfiI GAWTC 3 cut(s) 235, 265, 622
Tru1I TTAA 3 cut(s) 56, 365, 704
Tru9I TTAA 3 cut(s) 56, 365, 704
TseFI GTSAC 1 cut(s) 763
TseI GCWGC 5 cut(s) 4, 21, 153, 302, 715
Tsp45I GTSAC 1 cut(s) 763
TspDTI ATGAA 3 cut(s) 311, 491, 702
XapI RAATTY 1 cut(s) 497
XceI RCATGY 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.