Rw1G000650

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013498
Physical Location & Seq
Forward (+)
972405 .. 975292
2888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000650.1

Sequence Viewer

Length: 795 bp
ATGGACAGCAGCGGCTTGAAAACCCTAACCCCTTTCTCCCCTTTAAATCCATTTCGCTCCAGAGGAGTAGGACCTCCTCTCTTTGCTTCTCTATACTTGGCTCATCTCAACCGCAAATCCCGCCTCTCTCTTTCCTCCACTCGCTGCTCACTCTTGAATGATGTTGCAGCTGAGAGGGGCAAAGAGTGTTGTTCTTTGGCTCATGGGGCAGGGGAGGCACTCAAGCAAGTCTCACAAACTAAGATTCTTCAAGTCGTTCTCGTGTCTCCTCAGATTCCTGGGAATACAGGTTGCATTGCCAGAACATGTGCAGCATCAGCAGTTGGGCTTCATCTGGTTGGGCCGTTGGGCTTTCAGATTGATGATGCAAAATTAAAGCGTGCTGGATTGGACTATTGGCCATATGTGGTTGTCAAAGTTCACAGCTCATGGGCAGAGTTTAAAGACTATTTCAGGCAACAGGATGGTGAAAAGCGGTTGCTAGCATTTACAAAGAGAGCGACAAAATTTCATTCAGATTTTTCTTACAGAAGAGGTGATTTTCTCGTATTTGGGTCAGAAACCAGCGGCCTACCACCTGAAGCCCTCCAAGACTGCAAGAGTGGAACATTTGGCGGTGGAACACTTAGGATTCCAATGGTTGAAACATATGTAAGATGTCTCAATCTCTCTGTAAGTGTTGGCATTGCTTTATATGAAGCTTCGAGACAGTTAAATTATGAGCAGCTTCAATTTCCACTTGAAAATTGTATTGATAGTGAAAAATCAATTGTCACAGAGGACATTTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

264

Amino Acids

28.97

Weight (kDa)

8.75

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 83 - 232 5e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26880
fragaria_vesca FvH4_7g00690 FvH4_7g00690 FvH4_7g00690
malus_domestica MD07G1006600.v1.1
prunus_persica Prupe.2G009700_v2.0.a1 Prupe.2G009700_v2.0.a1
pyrus_communis pycom07g00530
rosa_chinensis RchiOBHm_Chr1g0314911
rosa_laevigata RLG00000030755
rosa_roxburghii Rroxscaffold_4G00331680
rosa_rugosa Rorug01G0002900 Rorug01G0003000
rosa_samantha Rh1AG013900 Rh1BG005500 Rh1CG012200 Rh1DG007300
rosa_wichuraiana Rw1G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 12, 112, 121, 475, 567, 615
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 1 cut(s) 506
AcuI CTGAAG 1 cut(s) 600
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 6 cut(s) 19, 157, 251, 644, 731, 743
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 4 cut(s) 170, 426, 701, 727
AluI AGCT 4 cut(s) 170, 426, 701, 727
Alw26I GTCTC 4 cut(s) 235, 270, 665, 700
AoxI GGCC 3 cut(s) 341, 398, 568
ApeKI GCWGC 5 cut(s) 9, 144, 167, 311, 724
ApoI RAATTY 1 cut(s) 506
AspS9I GGNCC 2 cut(s) 71, 341
AsuHPI GGTGA 2 cut(s) 479, 548
AsuNHI GCTAGC 1 cut(s) 481
AvaII GGWCC 1 cut(s) 71
BalI TGGCCA 1 cut(s) 400
BauI CACGAG 1 cut(s) 260
BbvI GCAGC 5 cut(s) 21, 131, 179, 323, 736
BccI CCATC 1 cut(s) 458
BceAI ACGGC 1 cut(s) 328
BciT130I CCWGG 1 cut(s) 279
BcoDI GTCTC 4 cut(s) 235, 270, 665, 700
BfaI CTAG 1 cut(s) 482
BisI GCNGC 7 cut(s) 10, 13, 145, 168, 312, 568, 725
BlsI GCNGC 7 cut(s) 11, 14, 146, 169, 313, 569, 726
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 71, 341
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 2 cut(s) 323, 355
BmtI GCTAGC 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 278
Bse3DI GCAATG 2 cut(s) 294, 684
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 278
BseGI GGATG 1 cut(s) 469
BseMI GCAATG 2 cut(s) 294, 684
BseMII CTCAG 2 cut(s) 162, 284
BseRI GAGGAG 3 cut(s) 66, 78, 258
BseXI GCAGC 5 cut(s) 21, 131, 179, 323, 736
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 3 cut(s) 343, 400, 570
BsmAI GTCTC 4 cut(s) 235, 270, 665, 700
BsnI GGCC 3 cut(s) 343, 400, 570
BspACI CCGC 6 cut(s) 12, 112, 121, 475, 567, 615
BspANI GGCC 3 cut(s) 343, 400, 570
BspCNI CTCAG 2 cut(s) 163, 283
BspOI GCTAGC 1 cut(s) 485
BsrDI GCAATG 2 cut(s) 294, 684
BssECI CCNNGG 1 cut(s) 278
BssSI CACGAG 1 cut(s) 260
Bst2BI CACGAG 1 cut(s) 260
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 711
Bst6I CTCTTC 1 cut(s) 526
BstC8I GCNNGC 2 cut(s) 381, 483
BstDEI CTNAG 4 cut(s) 171, 240, 270, 626
BstF5I GGATG 1 cut(s) 469
BstMAI GTCTC 4 cut(s) 235, 270, 665, 700
BstMWI GCNNNNNNNGC 4 cut(s) 120, 206, 215, 317
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 277
BstV1I GCAGC 5 cut(s) 21, 131, 179, 323, 736
BsuRI GGCC 3 cut(s) 343, 400, 570
BtsCI GGATG 1 cut(s) 469
Cac8I GCNNGC 2 cut(s) 381, 483
Cfr13I GGNCC 2 cut(s) 71, 341
CviAII CATG 3 cut(s) 203, 306, 429
DdeI CTNAG 4 cut(s) 171, 240, 270, 626
DraI TTTAAA 2 cut(s) 45, 442
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 526
EarI CTCTTC 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 277
FaeI CATG 3 cut(s) 206, 309, 432
FatI CATG 3 cut(s) 202, 305, 428
FauI CCCGC 1 cut(s) 128
FauNDI CATATG 2 cut(s) 403, 649
Fnu4HI GCNGC 7 cut(s) 10, 13, 145, 168, 312, 568, 725
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 7 cut(s) 10, 13, 145, 168, 312, 568, 725
FspBI CTAG 1 cut(s) 482
GluI GCNGC 7 cut(s) 10, 13, 145, 168, 312, 568, 725
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 3 cut(s) 343, 400, 570
Hin1II CATG 3 cut(s) 206, 309, 432
HindIII AAGCTT 1 cut(s) 699
HinfI GANTC 3 cut(s) 244, 274, 631
HphI GGTGA 2 cut(s) 479, 548
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 4 cut(s) 273, 357, 517, 559
Hpy188III TCNNGA 3 cut(s) 60, 154, 705
Hpy8I GTNNAC 1 cut(s) 421
HpyCH4III ACNGT 1 cut(s) 711
HpyCH4V TGCA 6 cut(s) 167, 294, 311, 368, 597, 791
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 206, 215, 317
HpyF3I CTNAG 4 cut(s) 171, 240, 270, 626
Hsp92II CATG 3 cut(s) 206, 309, 432
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 5 cut(s) 21, 131, 179, 323, 736
LweI GCATC 2 cut(s) 323, 355
MaeI CTAG 1 cut(s) 482
MaeIII GTNAC 1 cut(s) 772
MboII GAAGA 2 cut(s) 239, 543
MfeI CAATTG 1 cut(s) 768
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 6 cut(s) 371, 506, 715, 731, 745, 768
MluNI TGGCCA 1 cut(s) 400
Mox20I TGGCCA 1 cut(s) 400
MscI TGGCCA 1 cut(s) 400
MseI TTAA 4 cut(s) 44, 374, 441, 713
Msp20I TGGCCA 1 cut(s) 400
MspA1I CMGCKG 3 cut(s) 12, 170, 567
MspR9I CCNGG 1 cut(s) 279
MunI CAATTG 1 cut(s) 768
MvaI CCWGG 1 cut(s) 279
MwoI GCNNNNNNNGC 4 cut(s) 120, 206, 215, 317
NdeI CATATG 2 cut(s) 403, 649
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 3 cut(s) 206, 309, 432
NmuCI GTSAC 1 cut(s) 772
NspI RCATGY 1 cut(s) 309
PciI ACATGT 1 cut(s) 305
PfeI GAWTC 3 cut(s) 244, 274, 631
PkrI GCNGC 7 cut(s) 11, 14, 146, 169, 313, 569, 726
PpuMI RGGWCCY 1 cut(s) 71
PscI ACATGT 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspPI GGNCC 2 cut(s) 71, 341
PspPPI RGGWCCY 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 170
SaqAI TTAA 4 cut(s) 44, 374, 441, 713
SatI GCNGC 7 cut(s) 10, 13, 145, 168, 312, 568, 725
Sau96I GGNCC 2 cut(s) 71, 341
ScrFI CCNGG 1 cut(s) 279
SetI ASST 8 cut(s) 76, 172, 292, 428, 538, 580, 703, 729
SfaNI GCATC 2 cut(s) 323, 355
SinI GGWCC 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 6 cut(s) 371, 506, 715, 731, 745, 768
SsiI CCGC 6 cut(s) 12, 112, 121, 475, 567, 615
SspMI CTAG 1 cut(s) 482
StyD4I CCNGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 711
TaqI TCGA 1 cut(s) 704
TasI AATT 6 cut(s) 371, 506, 715, 731, 745, 768
TauI GCSGC 2 cut(s) 15, 570
TfiI GAWTC 3 cut(s) 244, 274, 631
Tru1I TTAA 4 cut(s) 44, 374, 441, 713
Tru9I TTAA 4 cut(s) 44, 374, 441, 713
TseFI GTSAC 1 cut(s) 772
TseI GCWGC 5 cut(s) 9, 144, 167, 311, 724
Tsp45I GTSAC 1 cut(s) 772
TspDTI ATGAA 3 cut(s) 320, 500, 711
VpaK11BI GGWCC 1 cut(s) 71
XapI RAATTY 1 cut(s) 506
XceI RCATGY 1 cut(s) 309
XspI CTAG 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.