RLG00000030755

SpoU rRNA Methylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013498
Physical Location & Seq
Reverse (-)
66926438 .. 66929417
2980 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030755

Sequence Viewer

Length: 783 bp
ATGGACAGCAGCGGCTTGAAAACCCTCACCCCTTTCTCTCCTAATCCATTTCGCTCCAGAGGAGGAGCTCCTCTCTTTGCTTCTCTATACTTGGCTCATCTCAACCGCAAATCCCGCCTCTCTCTTTCCTCCACTCGCTTCTCTCCCCGCTGCTCACTCTCTGAGAGGGGCAAAGTGTGTTCTTTGGCTCGTGGGGCAGGGGAGGCACTCAAGCAAGTCTCACAAACTAAGATTCTTCAAGTGGTTCTCGTGTCTCCTCAGATTCCTGGAAATACAGGTTGCATTGCCAGAACATGTGCAGCATCAGCAGTTGGGCTTCATCTGGTTGGGCCGTTGGGCTTTCAGATTGATGATGCAAAATTAAAGCGTGCTGGATTGGACTATTGGCCATATGTGGTTGTCAAAGTTCACAACTCATGGGCAGAGTTTCAAGACTATTTCAGGCAACAGGATGGTGAAAAGCGGTTGCTTGCATTTACAAAGAGAGCGACAAAATTTCATTCGGATTTTTCTTACAGAAGAGGTGATTTTCTTGTATTTGGGTCAGAAACCAGCGGCCTACCACCTGAAGCCCTCCAAGACTGCAAGAGTGGAACATTTGGCGGTGGAACACTTAGGATTCCAATGGTTGAAACGTATGTAAGATGTCTCAATCTCTCTGTAAGTGTTGGCATTGCTTTATATGAAGCTTCGAGACAGTTAAATTATGAGCAGCTTCAATTTCCACTTGAAAATTGTCTTGATGGTGAAAAATCTGTTGTCACAGAGGACATTTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

261

Amino Acids

28.6

Weight (kDa)

9.18

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 79 - 228 3e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26880
fragaria_vesca FvH4_7g00690 FvH4_7g00690 FvH4_7g00690
malus_domestica MD07G1006600.v1.1
prunus_persica Prupe.2G009700_v2.0.a1 Prupe.2G009700_v2.0.a1
pyrus_communis pycom07g00530
rosa_chinensis RchiOBHm_Chr1g0314911
rosa_laevigata RLG00000030755
rosa_roxburghii Rroxscaffold_4G00331680
rosa_rugosa Rorug01G0002900 Rorug01G0003000
rosa_samantha Rh1AG013900 Rh1BG005500 Rh1CG012200 Rh1DG007300
rosa_wichuraiana Rw1G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 12, 106, 115, 148, 463, 555, 603
AcoI YGGCCR 1 cut(s) 386
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 588
AflIII ACRYGT 1 cut(s) 293
AgsI TTSAA 6 cut(s) 19, 239, 431, 632, 719, 731
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 3 cut(s) 68, 689, 715
AluI AGCT 3 cut(s) 68, 689, 715
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 4 cut(s) 223, 258, 653, 688
AoxI GGCC 3 cut(s) 329, 386, 556
ApeKI GCWGC 4 cut(s) 9, 150, 299, 712
ApoI RAATTY 1 cut(s) 494
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 4 cut(s) 19, 467, 536, 758
BalI TGGCCA 1 cut(s) 388
BanII GRGCYC 1 cut(s) 70
BauI CACGAG 2 cut(s) 189, 248
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 4 cut(s) 21, 137, 311, 724
BccI CCATC 2 cut(s) 446, 737
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 267
BcoDI GTCTC 4 cut(s) 223, 258, 653, 688
BisI GCNGC 6 cut(s) 10, 13, 151, 300, 556, 713
BlsI GCNGC 6 cut(s) 11, 14, 152, 301, 557, 714
Bme1390I CCNGG 1 cut(s) 267
BmgT120I GGNCC 1 cut(s) 329
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 2 cut(s) 311, 343
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 194
Bse3DI GCAATG 2 cut(s) 282, 672
BseBI CCWGG 1 cut(s) 267
BseGI GGATG 1 cut(s) 457
BseMI GCAATG 2 cut(s) 282, 672
BseMII CTCAG 2 cut(s) 153, 272
BseRI GAGGAG 4 cut(s) 60, 75, 78, 246
BseXI GCAGC 4 cut(s) 21, 137, 311, 724
BsgI GTGCAG 1 cut(s) 318
BshFI GGCC 3 cut(s) 331, 388, 558
BsiHKAI GWGCWC 1 cut(s) 70
BsmAI GTCTC 4 cut(s) 223, 258, 653, 688
BsnI GGCC 3 cut(s) 331, 388, 558
Bsp1286I GDGCHC 1 cut(s) 70
BspACI CCGC 7 cut(s) 12, 106, 115, 148, 463, 555, 603
BspANI GGCC 3 cut(s) 331, 388, 558
BspCNI CTCAG 2 cut(s) 154, 271
BsrDI GCAATG 2 cut(s) 282, 672
BssSI CACGAG 2 cut(s) 189, 248
Bst2BI CACGAG 2 cut(s) 189, 248
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 699
Bst6I CTCTTC 1 cut(s) 514
BstC8I GCNNGC 2 cut(s) 369, 471
BstDEI CTNAG 4 cut(s) 162, 228, 258, 614
BstF5I GGATG 1 cut(s) 457
BstMAI GTCTC 4 cut(s) 223, 258, 653, 688
BstMWI GCNNNNNNNGC 4 cut(s) 114, 194, 203, 305
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 265
BstV1I GCAGC 4 cut(s) 21, 137, 311, 724
BsuRI GGCC 3 cut(s) 331, 388, 558
BtsCI GGATG 1 cut(s) 457
Cac8I GCNNGC 2 cut(s) 369, 471
Cfr13I GGNCC 1 cut(s) 329
CviAII CATG 2 cut(s) 294, 417
DdeI CTNAG 4 cut(s) 162, 228, 258, 614
EaeI YGGCCR 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 514
Ecl136II GAGCTC 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 70
FaeI CATG 2 cut(s) 297, 420
FatI CATG 2 cut(s) 293, 416
FauI CCCGC 2 cut(s) 122, 155
FauNDI CATATG 1 cut(s) 391
Fnu4HI GCNGC 6 cut(s) 10, 13, 151, 300, 556, 713
FokI GGATG 1 cut(s) 464
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 6 cut(s) 10, 13, 151, 300, 556, 713
GluI GCNGC 6 cut(s) 10, 13, 151, 300, 556, 713
GsuI CTGGAG 1 cut(s) 40
HaeIII GGCC 3 cut(s) 331, 388, 558
Hin1II CATG 2 cut(s) 297, 420
HindIII AAGCTT 1 cut(s) 687
HinfI GANTC 3 cut(s) 232, 262, 619
HphI GGTGA 4 cut(s) 19, 467, 536, 758
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 5 cut(s) 163, 261, 345, 505, 547
Hpy188III TCNNGA 4 cut(s) 57, 431, 693, 740
Hpy8I GTNNAC 1 cut(s) 409
HpyCH4III ACNGT 1 cut(s) 699
HpyCH4IV ACGT 1 cut(s) 635
HpyCH4V TGCA 6 cut(s) 282, 299, 356, 473, 585, 779
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 194, 203, 305
HpyF3I CTNAG 4 cut(s) 162, 228, 258, 614
HpySE526I ACGT 1 cut(s) 635
Hsp92II CATG 2 cut(s) 297, 420
LmnI GCTCC 3 cut(s) 59, 65, 73
Lsp1109I GCAGC 4 cut(s) 21, 137, 311, 724
LweI GCATC 2 cut(s) 311, 343
MaeII ACGT 1 cut(s) 635
MaeIII GTNAC 1 cut(s) 760
MboII GAAGA 2 cut(s) 227, 531
MhlI GDGCHC 1 cut(s) 70
MlsI TGGCCA 1 cut(s) 388
MluCI AATT 5 cut(s) 359, 494, 703, 719, 733
MluNI TGGCCA 1 cut(s) 388
Mox20I TGGCCA 1 cut(s) 388
MscI TGGCCA 1 cut(s) 388
MseI TTAA 2 cut(s) 362, 701
Msp20I TGGCCA 1 cut(s) 388
MspA1I CMGCKG 3 cut(s) 12, 150, 555
MspR9I CCNGG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 4 cut(s) 114, 194, 203, 305
NdeI CATATG 1 cut(s) 391
NlaIII CATG 2 cut(s) 297, 420
NmuCI GTSAC 1 cut(s) 760
NspI RCATGY 1 cut(s) 297
PciI ACATGT 1 cut(s) 293
PfeI GAWTC 3 cut(s) 232, 262, 619
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 6 cut(s) 11, 14, 152, 301, 557, 714
PscI ACATGT 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspPI GGNCC 1 cut(s) 329
SacI GAGCTC 1 cut(s) 70
SaqAI TTAA 2 cut(s) 362, 701
SatI GCNGC 6 cut(s) 10, 13, 151, 300, 556, 713
Sau96I GGNCC 1 cut(s) 329
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 1 cut(s) 70
SetI ASST 7 cut(s) 70, 280, 526, 568, 638, 691, 717
SfaNI GCATC 2 cut(s) 311, 343
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 5 cut(s) 359, 494, 703, 719, 733
SsiI CCGC 7 cut(s) 12, 106, 115, 148, 463, 555, 603
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 699
TaiI ACGT 1 cut(s) 638
TaqI TCGA 1 cut(s) 692
TasI AATT 5 cut(s) 359, 494, 703, 719, 733
TauI GCSGC 2 cut(s) 15, 558
TfiI GAWTC 3 cut(s) 232, 262, 619
Tru1I TTAA 2 cut(s) 362, 701
Tru9I TTAA 2 cut(s) 362, 701
TseFI GTSAC 1 cut(s) 760
TseI GCWGC 4 cut(s) 9, 150, 299, 712
Tsp45I GTSAC 1 cut(s) 760
TspDTI ATGAA 3 cut(s) 308, 488, 699
XapI RAATTY 1 cut(s) 494
XceI RCATGY 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.