MD07G1115500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011176
Physical Location & Seq
Forward (+)
13922170 .. 13924895
2726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1115500.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGCATCATTAGCTCAACCTCCATTCATGATGTCTGCCAATATTGTTCGCAGAGTCCACCCTGCAATGCAGAAATCAGTGAAAACACCAAATACAATACATATGCCCACCTTGTCTCACATTTGGATAAAACTTTCCCCTGTTTGCTGCACAAAACTGACTCGCTTGGAGCCCTCATCTGCCTCATACGCCCCCACGGAGAGTGCTGGTGACAACTTCTTGAATAAAACCTCCAACATATTTGAAACCCTTTCTTCTATTAGCAATGATGAAGCACCAGAAACCAACAGTGAAACGCATACTGATGCAAGTAATCAGACAAAAACGGAGTTTCAGGTCCTCAAATGGCCAATGTGGCTCTTGGGCCCTTCTCTTCTCCTCATCACCGGCATGGTGCCAACTTTGTGGCTACCAATATCTTCCATATTCCTAGGCCCCAACATAGCCAGCCTGCTTTCCTTGGTCGGACTTGATTGTATATATAATCTTGGCGCAACCCTTTTTCTACTCATGGCCGATTCTTGTGCTCGGCCTAAACAGCCAGAACAAGCCTGTAAAAGCAAGCCCCCATTTAGCTACCAGTTTTGGAATATAGTTGCAACCGTGTCTGGATTTATCGTTCCCCTAATAATGGCTTTTGGATCCCAAAAGGGTTTCCTCCAACCCCAGCTGCCCTTCATCTCATTTGCAGTTCTATTGGGTCCCTACCTTCTGCTTATTTCAGTACAGATTCTGACAGAGATGCTGACTTGGCATTGGCAGTCACCGGTTTGGCTTGTGACCCCTGTAGTGTACGAGTCTTACCGGCTGTTGCAGCTGATGAGGGGATTGAAGCTTGGAACTGAGCTCAGTGCACCAGCATGGATGATGCATACGATCAGAGGACTAGTGTCGTGGTGGGTACTGATTCTTGGCATGCAGCTGATGAGAGTTGCTTGGTTTGCTGGTTTCAGCGCTCGAACTCGTAAGCAGGAGCCGCCTTCTGCTGATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

332

Amino Acids

36.94

Weight (kDa)

8.79

Isoelectric Point (pI)

55.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60590 AT3G60590 AT3G60590 AT3G60590
fragaria_vesca FvH4_7g11080 FvH4_7g11080
malus_domestica MD07G1115500.v1.1
prunus_persica Prupe.2G138000_v2.0.a1
pyrus_communis pycom07g10900
rosa_chinensis RchiOBHm_Chr1g0349101
rosa_laevigata RLG00000028606
rosa_multiflora Rmu_sc0008324.1_g000003 Rmu_sc0012100.1_g000004
rosa_roxburghii Rroxscaffold_4G00306120
rosa_rugosa Rorug01G0200100
rosa_samantha Rh1AG217800 Rh1BG183300 Rh1CG202200 Rh1DG213100
rosa_wichuraiana Rw1G018510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AciI CCGC 1 cut(s) 977
AclWI GGATC 2 cut(s) 636, 649
AcoI YGGCCR 2 cut(s) 347, 513
AfaI GTAC 3 cut(s) 726, 794, 903
AfeI AGCGCT 1 cut(s) 955
AfiI CCNNNNNNNGG 1 cut(s) 631
AgeI ACCGGT 1 cut(s) 766
AgsI TTSAA 3 cut(s) 223, 245, 832
AhlI ACTAGT 1 cut(s) 886
AloI GAACNNNNNNTCC 2 cut(s) 603, 635
AluBI AGCT 7 cut(s) 14, 576, 670, 817, 835, 847, 922
AluI AGCT 7 cut(s) 14, 576, 670, 817, 835, 847, 922
Alw21I GWGCWC 3 cut(s) 529, 849, 856
Alw26I GTCTC 1 cut(s) 120
Alw44I GTGCAC 1 cut(s) 852
AlwI GGATC 2 cut(s) 636, 649
AlwNI CAGNNNCTG 1 cut(s) 733
Aor51HI AGCGCT 1 cut(s) 955
AoxI GGCC 5 cut(s) 347, 364, 433, 513, 530
ApaI GGGCCC 1 cut(s) 368
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 4 cut(s) 147, 670, 814, 919
AsiGI ACCGGT 1 cut(s) 766
AspA2I CCTAGG 1 cut(s) 430
AspLEI GCGC 2 cut(s) 494, 956
AspS9I GGNCC 5 cut(s) 337, 364, 365, 434, 701
AsuHPI GGTGA 3 cut(s) 221, 376, 756
AvaII GGWCC 2 cut(s) 337, 701
AvrII CCTAGG 1 cut(s) 430
BaeGI GKGCMC 2 cut(s) 368, 856
BalI TGGCCA 1 cut(s) 349
BamHI GGATCC 1 cut(s) 641
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 3 cut(s) 174, 368, 849
Bbv12I GWGCWC 3 cut(s) 529, 849, 856
BbvI GCAGC 4 cut(s) 134, 657, 826, 931
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BcoDI GTCTC 1 cut(s) 120
BcuI ACTAGT 1 cut(s) 886
BfaI CTAG 3 cut(s) 431, 887, 994
BfmI CTRYAG 1 cut(s) 786
BfoI RGCGCY 1 cut(s) 957
BglI GCCNNNNNGGC 1 cut(s) 355
BisI GCNGC 5 cut(s) 148, 671, 815, 920, 977
BlnI CCTAGG 1 cut(s) 430
BlsI GCNGC 5 cut(s) 149, 672, 816, 921, 978
Bme18I GGWCC 2 cut(s) 337, 701
BmgT120I GGNCC 5 cut(s) 337, 364, 365, 434, 701
BmiI GGNNCC 8 cut(s) 171, 366, 396, 436, 643, 702, 703, 975
BmsI GCATC 4 cut(s) 14, 295, 732, 858
BoxI GACNNNNGTC 1 cut(s) 889
BsaJI CCNNGG 3 cut(s) 195, 430, 459
BsaWI WCCGGW 1 cut(s) 766
Bsc4I CCNNNNNNNGG 1 cut(s) 631
Bse118I RCCGGY 3 cut(s) 386, 766, 804
Bse1I ACTGG 1 cut(s) 580
Bse3DI GCAATG 2 cut(s) 72, 271
BseDI CCNNGG 3 cut(s) 195, 430, 459
BseGI GGATG 1 cut(s) 870
BseLI CCNNNNNNNGG 1 cut(s) 631
BseMI GCAATG 2 cut(s) 72, 271
BseMII CTCAG 2 cut(s) 834, 862
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 2 cut(s) 368, 856
BseXI GCAGC 4 cut(s) 134, 657, 826, 931
BseYI CCCAGC 1 cut(s) 666
BsgI GTGCAG 1 cut(s) 133
BshFI GGCC 5 cut(s) 349, 366, 435, 515, 532
BshNI GGYRCC 1 cut(s) 394
BshTI ACCGGT 1 cut(s) 766
BsiHKAI GWGCWC 3 cut(s) 529, 849, 856
BsiSI CCGG 3 cut(s) 387, 767, 805
BslFI GGGAC 1 cut(s) 687
BslI CCNNNNNNNGG 1 cut(s) 631
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 687
BsnI GGCC 5 cut(s) 349, 366, 435, 515, 532
Bsp120I GGGCCC 1 cut(s) 364
Bsp1286I GDGCHC 5 cut(s) 174, 368, 529, 849, 856
Bsp143I GATC 2 cut(s) 641, 876
BspACI CCGC 1 cut(s) 977
BspANI GGCC 5 cut(s) 349, 366, 435, 515, 532
BspCNI CTCAG 2 cut(s) 835, 861
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 8 cut(s) 171, 366, 396, 436, 643, 702, 703, 975
BspPI GGATC 2 cut(s) 636, 649
BspT107I GGYRCC 1 cut(s) 394
BsrDI GCAATG 2 cut(s) 72, 271
BsrFI RCCGGY 3 cut(s) 386, 766, 804
BsrI ACTGG 1 cut(s) 580
BssAI RCCGGY 3 cut(s) 386, 766, 804
BssECI CCNNGG 3 cut(s) 195, 430, 459
BssMI GATC 2 cut(s) 641, 876
BssT1I CCWWGG 2 cut(s) 430, 459
Bst4CI ACNGT 2 cut(s) 290, 604
Bst6I CTCTTC 1 cut(s) 378
BstC8I GCNNGC 4 cut(s) 448, 452, 563, 917
BstDEI CTNAG 2 cut(s) 843, 848
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 870
BstH2I RGCGCY 1 cut(s) 957
BstHHI GCGC 2 cut(s) 494, 956
BstKTI GATC 2 cut(s) 644, 879
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 2 cut(s) 641, 876
BstMWI GCNNNNNNNGC 8 cut(s) 11, 188, 355, 538, 751, 814, 941, 976
BstNSI RCATGY 1 cut(s) 919
BstPAI GACNNNNGTC 1 cut(s) 889
BstSFI CTRYAG 1 cut(s) 786
BstSLI GKGCMC 2 cut(s) 368, 856
BstV1I GCAGC 4 cut(s) 134, 657, 826, 931
BstX2I RGATCY 1 cut(s) 641
BstXI CCANNNNNNTGG 1 cut(s) 405
BstYI RGATCY 1 cut(s) 641
BsuRI GGCC 5 cut(s) 349, 366, 435, 515, 532
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 1 cut(s) 870
BtsIMutI CAGTG 3 cut(s) 84, 295, 856
Cac8I GCNNGC 4 cut(s) 448, 452, 563, 917
CaiI CAGNNNCTG 1 cut(s) 733
CciI TCATGA 1 cut(s) 27
CfoI GCGC 2 cut(s) 494, 956
Cfr10I RCCGGY 3 cut(s) 386, 766, 804
Cfr13I GGNCC 5 cut(s) 337, 364, 365, 434, 701
Csp6I GTAC 3 cut(s) 725, 793, 902
CspAI ACCGGT 1 cut(s) 766
CviAII CATG 5 cut(s) 28, 391, 511, 861, 916
CviQI GTAC 3 cut(s) 725, 793, 902
DdeI CTNAG 2 cut(s) 843, 848
DpnI GATC 2 cut(s) 643, 878
DpnII GATC 2 cut(s) 641, 876
EaeI YGGCCR 2 cut(s) 347, 513
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Ecl136II GAGCTC 1 cut(s) 847
Eco130I CCWWGG 2 cut(s) 430, 459
Eco24I GRGCYC 3 cut(s) 174, 368, 849
Eco47I GGWCC 2 cut(s) 337, 701
Eco47III AGCGCT 1 cut(s) 955
Eco53kI GAGCTC 1 cut(s) 847
EcoICRI GAGCTC 1 cut(s) 847
EcoO109I RGGNCCY 4 cut(s) 337, 365, 434, 701
EcoT14I CCWWGG 2 cut(s) 430, 459
EcoT22I ATGCAT 1 cut(s) 873
EcoT38I GRGCYC 3 cut(s) 174, 368, 849
ErhI CCWWGG 2 cut(s) 430, 459
FaeI CATG 5 cut(s) 31, 394, 514, 864, 919
FaqI GGGAC 1 cut(s) 687
FatI CATG 5 cut(s) 27, 390, 510, 860, 915
FauNDI CATATG 1 cut(s) 102
Fnu4HI GCNGC 5 cut(s) 148, 671, 815, 920, 977
FokI GGATG 1 cut(s) 877
FriOI GRGCYC 3 cut(s) 174, 368, 849
Fsp4HI GCNGC 5 cut(s) 148, 671, 815, 920, 977
FspBI CTAG 3 cut(s) 431, 887, 994
GlaI GCGC 2 cut(s) 493, 955
GluI GCNGC 5 cut(s) 148, 671, 815, 920, 977
GsaI CCCAGC 1 cut(s) 670
HaeII RGCGCY 1 cut(s) 957
HaeIII GGCC 5 cut(s) 349, 366, 435, 515, 532
HapII CCGG 3 cut(s) 387, 767, 805
HhaI GCGC 2 cut(s) 494, 956
Hin1II CATG 5 cut(s) 31, 394, 514, 864, 919
Hin6I GCGC 2 cut(s) 492, 954
HinP1I GCGC 2 cut(s) 492, 954
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 6 cut(s) 54, 160, 518, 730, 797, 907
HpaII CCGG 3 cut(s) 387, 767, 805
HphI GGTGA 3 cut(s) 221, 376, 756
Hpy166II GTNNAC 3 cut(s) 58, 793, 854
Hpy188I TCNGA 4 cut(s) 318, 467, 735, 881
Hpy188III TCNNGA 3 cut(s) 28, 220, 609
Hpy8I GTNNAC 3 cut(s) 58, 793, 854
HpyAV CCTTC 4 cut(s) 378, 685, 719, 990
HpyCH4III ACNGT 2 cut(s) 290, 604
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 188, 355, 538, 751, 814, 941, 976
HpyF3I CTNAG 2 cut(s) 843, 848
Hsp92II CATG 5 cut(s) 31, 394, 514, 864, 919
HspAI GCGC 2 cut(s) 492, 954
KflI GGGWCCC 1 cut(s) 701
Kzo9I GATC 2 cut(s) 641, 876
LmnI GCTCC 2 cut(s) 169, 973
Lsp1109I GCAGC 4 cut(s) 134, 657, 826, 931
LweI GCATC 4 cut(s) 14, 295, 732, 858
MaeI CTAG 3 cut(s) 431, 887, 994
MaeIII GTNAC 3 cut(s) 209, 762, 778
MalI GATC 2 cut(s) 643, 878
MboI GATC 2 cut(s) 641, 876
MboII GAAGA 3 cut(s) 246, 365, 411
MflI RGATCY 1 cut(s) 641
MhlI GDGCHC 5 cut(s) 174, 368, 529, 849, 856
MlsI TGGCCA 1 cut(s) 349
MluNI TGGCCA 1 cut(s) 349
MlyI GAGTC 3 cut(s) 63, 154, 806
MmeI TCCRAC 3 cut(s) 258, 445, 685
MnlI CCTC 9 cut(s) 30, 184, 193, 241, 350, 389, 668, 816, 875
Mox20I TGGCCA 1 cut(s) 349
Mph1103I ATGCAT 1 cut(s) 873
MscI TGGCCA 1 cut(s) 349
MslI CAYNNNNRTG 3 cut(s) 303, 389, 859
Msp20I TGGCCA 1 cut(s) 349
MspA1I CMGCKG 3 cut(s) 670, 817, 922
MspI CCGG 3 cut(s) 387, 767, 805
MwoI GCNNNNNNNGC 8 cut(s) 11, 188, 355, 538, 751, 814, 941, 976
NdeI CATATG 1 cut(s) 102
NdeII GATC 2 cut(s) 641, 876
NlaIII CATG 5 cut(s) 31, 394, 514, 864, 919
NlaIV GGNNCC 8 cut(s) 171, 366, 396, 436, 643, 702, 703, 975
NmeAIII GCCGAG 1 cut(s) 508
NmuCI GTSAC 3 cut(s) 209, 762, 778
NsiI ATGCAT 1 cut(s) 873
NspI RCATGY 1 cut(s) 919
PaeI GCATGC 1 cut(s) 919
PagI TCATGA 1 cut(s) 27
PfeI GAWTC 3 cut(s) 518, 730, 907
PinAI ACCGGT 1 cut(s) 766
PkrI GCNGC 5 cut(s) 149, 672, 816, 921, 978
PleI GAGTC 3 cut(s) 62, 154, 805
PpsI GAGTC 3 cut(s) 62, 154, 805
PpuMI RGGWCCY 2 cut(s) 337, 701
PshAI GACNNNNGTC 1 cut(s) 889
Psp124BI GAGCTC 1 cut(s) 849
Psp5II RGGWCCY 2 cut(s) 337, 701
PspFI CCCAGC 1 cut(s) 666
PspN4I GGNNCC 8 cut(s) 171, 366, 396, 436, 643, 702, 703, 975
PspOMI GGGCCC 1 cut(s) 364
PspPI GGNCC 5 cut(s) 337, 364, 365, 434, 701
PspPPI RGGWCCY 2 cut(s) 337, 701
PstNI CAGNNNCTG 1 cut(s) 733
PsuI RGATCY 1 cut(s) 641
PvuII CAGCTG 3 cut(s) 670, 817, 922
RsaI GTAC 3 cut(s) 726, 794, 903
RsaNI GTAC 3 cut(s) 725, 793, 902
RseI CAYNNNNRTG 3 cut(s) 303, 389, 859
SacI GAGCTC 1 cut(s) 849
SatI GCNGC 5 cut(s) 148, 671, 815, 920, 977
Sau3AI GATC 2 cut(s) 641, 876
Sau96I GGNCC 5 cut(s) 337, 364, 365, 434, 701
SchI GAGTC 3 cut(s) 63, 154, 806
SduI GDGCHC 5 cut(s) 174, 368, 529, 849, 856
SfaNI GCATC 4 cut(s) 14, 295, 732, 858
SfcI CTRYAG 1 cut(s) 786
SinI GGWCC 2 cut(s) 337, 701
SmiMI CAYNNNNRTG 3 cut(s) 303, 389, 859
SpeI ACTAGT 1 cut(s) 886
SphI GCATGC 1 cut(s) 919
SsiI CCGC 1 cut(s) 977
SspI AATATT 1 cut(s) 43
SspMI CTAG 3 cut(s) 431, 887, 994
SstI GAGCTC 1 cut(s) 849
StyI CCWWGG 2 cut(s) 430, 459
TaaI ACNGT 2 cut(s) 290, 604
TaqI TCGA 1 cut(s) 958
TatI WGTACW 1 cut(s) 724
TauI GCSGC 1 cut(s) 979
TfiI GAWTC 3 cut(s) 518, 730, 907
TscAI CASTG 3 cut(s) 84, 295, 856
TseFI GTSAC 3 cut(s) 209, 762, 778
TseI GCWGC 4 cut(s) 147, 670, 814, 919
Tsp45I GTSAC 3 cut(s) 209, 762, 778
TspDTI ATGAA 3 cut(s) 16, 285, 667
TspGWI ACGGA 2 cut(s) 212, 341
TspRI CASTG 3 cut(s) 84, 295, 856
VneI GTGCAC 1 cut(s) 852
VpaK11BI GGWCC 2 cut(s) 337, 701
XceI RCATGY 1 cut(s) 919
XmaJI CCTAGG 1 cut(s) 430
XspI CTAG 3 cut(s) 431, 887, 994
Zsp2I ATGCAT 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.