MD07G1128700.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004718
Physical Location & Seq
Reverse (-)
18390508 .. 18396769
6262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1128700.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGCACCCATGAACTCGGCACCTCTTCTTCTTCGGCAATCTCTCCCGCCAATATCATCCCCAATCGAAACTCTGAAACCGAAAACTCCACTGCCAGTGCACAATAGCACGCTCGGCGCACGAACACCTGCATATTCACCTTGTTATATAAGAGTTTGCACTGGTAAAACAACCAGAGGAAGTGGAAGTGGAAGACGAAGCAGAAGAAACCCAGATGACTACCATGCCACTCTCACAGCTCTCAATTCCAAAGGCCGATTTCCCAGAAAATCCCTCGGCCAGCATTATATGTTGGACTCTGAGATCAACGATGAGCTCACTGCTGCGGCCGATGTTGGGGAAGGTGATGTGGTGTTAGAAATTGGGCCGGGAACAGGTTCTTTGACTAATACTCTTATTAGCGCTGGTGCAGTTGTGCTCGCAATCGAAAAGGATTCACACATGGCTACTCTTGTGAGTGAAAGATTTGCGCAGACTGACCGGTTGAAGGTTTTGAATGAGGACTTTGTAAAATGCCACATTCACTCACATATGTCGTCCTTATTGGGAAGTATAGAGCCATCTGGTCCAAATTCAAGACTTGCAAAAGTAGTTGCTAATATACCCTTTAATATAAGTACAGATGTTGTCAAACAACTTCTCCCAATGGGGGACATCTTTTCTGAAGTTGTTCTCTTACTCCAGGAGGAGGCAGCTTTGCGCTTGGTGGAACCATCTTTGAGGACATCCGAGTACCGGCCCATCAGTATCTTCGTCAATTTCTTTTCAGATCCTGAATTCATAATGAAGGTCCCAAGGACAAAATTTTTTCCTCAGCCTAATGTTGATGCTGCCGTTGTTAAATTTAAACTGAAGCAACCTGTAGACTACCCCCCAGTTTCCTCTACTAAAAGCTTCTTCTCAATGGTCAATTCTGCTTTTAATGGGAAACGAAAGATGTTGCGGAGATCACTCCAGCACATATGCACTCCCATGGAGATTGAAAATGCTTTGGGAACGGTTGGTCGTCCAGCCACGTCAAGACCAGAGGAGCTATCCACGGATGATTTTGTGAAGTTGCACAATTTGATCACGAAACAATAA

Protein Analysis

361

Amino Acids

39.58

Weight (kDa)

9.2

Isoelectric Point (pI)

63.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RrnaAD PF00398 89 - 357 1.7e-47 Ribosomal RNA adenine dimethylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 136
Acc16I TGCGCA 1 cut(s) 471
Acc36I ACCTGC 1 cut(s) 136
AccB1I GGYRCC 2 cut(s) 4, 19
AccI GTMKAC 1 cut(s) 864
AciI CCGC 3 cut(s) 47, 326, 943
AclWI GGATC 1 cut(s) 764
AcoI YGGCCR 2 cut(s) 277, 327
AcsI RAATTY 4 cut(s) 571, 776, 803, 842
AcuI CTGAAG 2 cut(s) 684, 872
AfaI GTAC 2 cut(s) 619, 734
AfeI AGCGCT 1 cut(s) 403
AfiI CCNNNNNNNGG 6 cut(s) 336, 374, 487, 649, 688, 735
AgeI ACCGGT 1 cut(s) 480
AgsI TTSAA 4 cut(s) 487, 496, 576, 983
AjiI CACGTC 1 cut(s) 1017
AjnI CCWGG 1 cut(s) 681
AluBI AGCT 5 cut(s) 239, 316, 695, 894, 1033
AluI AGCT 5 cut(s) 239, 316, 695, 894, 1033
Alw21I GWGCWC 3 cut(s) 102, 318, 420
Alw44I GTGCAC 1 cut(s) 98
AlwI GGATC 1 cut(s) 764
AlwNI CAGNNNCTG 1 cut(s) 773
Aor51HI AGCGCT 1 cut(s) 403
AoxI GGCC 5 cut(s) 253, 277, 327, 365, 737
ApaLI GTGCAC 1 cut(s) 98
ApeKI GCWGC 3 cut(s) 323, 692, 830
ApoI RAATTY 4 cut(s) 571, 776, 803, 842
AsiGI ACCGGT 1 cut(s) 480
Asp700I GAANNNNTTC 2 cut(s) 376, 669
AspLEI GCGC 4 cut(s) 119, 404, 472, 702
AspS9I GGNCC 4 cut(s) 365, 566, 738, 790
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 2 cut(s) 129, 356
AvaII GGWCC 2 cut(s) 566, 790
BaeGI GKGCMC 1 cut(s) 102
BanI GGYRCC 2 cut(s) 4, 19
BanII GRGCYC 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 3 cut(s) 102, 318, 420
BbvCI CCTCAGC 1 cut(s) 813
BbvI GCAGC 3 cut(s) 310, 704, 817
BccI CCATC 3 cut(s) 568, 721, 749
BceAI ACGGC 1 cut(s) 818
BciT130I CCWGG 1 cut(s) 683
BclI TGATCA 1 cut(s) 1068
BcnI CCSGG 1 cut(s) 369
BfmI CTRYAG 1 cut(s) 861
BfoI RGCGCY 1 cut(s) 405
BfuAI ACCTGC 1 cut(s) 136
BisI GCNGC 4 cut(s) 324, 327, 693, 831
BlsI GCNGC 4 cut(s) 325, 328, 694, 832
Bme1390I CCNGG 2 cut(s) 369, 683
Bme18I GGWCC 2 cut(s) 566, 790
BmgBI CACGTC 1 cut(s) 1017
BmgT120I GGNCC 4 cut(s) 365, 566, 738, 790
BmiI GGNNCC 4 cut(s) 6, 21, 711, 792
BmrFI CCNGG 2 cut(s) 369, 683
BmrI ACTGGG 1 cut(s) 869
BmsI GCATC 1 cut(s) 817
BmuI ACTGGG 1 cut(s) 869
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 2 cut(s) 665, 938
Bpu10I CCTNAGC 1 cut(s) 813
BpuMI CCSGG 1 cut(s) 369
BsaJI CCNNGG 4 cut(s) 274, 794, 972, 1038
BsaWI WCCGGW 1 cut(s) 480
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bsc4I CCNNNNNNNGG 6 cut(s) 336, 374, 487, 649, 688, 735
Bse118I RCCGGY 2 cut(s) 480, 735
Bse1I ACTGG 3 cut(s) 95, 166, 875
BseBI CCWGG 1 cut(s) 683
BseDI CCNNGG 4 cut(s) 274, 794, 972, 1038
BseGI GGATG 3 cut(s) 56, 725, 1048
BseLI CCNNNNNNNGG 6 cut(s) 336, 374, 487, 649, 688, 735
BseMII CTCAG 2 cut(s) 291, 827
BseNI ACTGG 3 cut(s) 95, 166, 875
BseRI GAGGAG 2 cut(s) 701, 1043
BseSI GKGCMC 1 cut(s) 102
BseX3I CGGCCG 1 cut(s) 327
BseXI GCAGC 3 cut(s) 310, 704, 817
BsgI GTGCAG 1 cut(s) 429
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 5 cut(s) 255, 279, 329, 367, 739
BshNI GGYRCC 2 cut(s) 4, 19
BshTI ACCGGT 1 cut(s) 480
BsiEI CGRYCG 1 cut(s) 330
BsiHKAI GWGCWC 3 cut(s) 102, 318, 420
BsiSI CCGG 3 cut(s) 368, 481, 736
BslFI GGGAC 2 cut(s) 665, 776
BslI CCNNNNNNNGG 6 cut(s) 336, 374, 487, 649, 688, 735
BsmFI GGGAC 2 cut(s) 665, 776
BsnI GGCC 5 cut(s) 255, 279, 329, 367, 739
Bsp1286I GDGCHC 3 cut(s) 102, 318, 420
Bsp143I GATC 4 cut(s) 303, 769, 947, 1068
Bsp19I CCATGG 1 cut(s) 972
BspACI CCGC 3 cut(s) 47, 326, 943
BspANI GGCC 5 cut(s) 255, 279, 329, 367, 739
BspCNI CTCAG 2 cut(s) 292, 826
BspLI GGNNCC 4 cut(s) 6, 21, 711, 792
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 1 cut(s) 764
BspT107I GGYRCC 2 cut(s) 4, 19
BsrFI RCCGGY 2 cut(s) 480, 735
BsrI ACTGG 3 cut(s) 95, 166, 875
BssAI RCCGGY 2 cut(s) 480, 735
BssECI CCNNGG 4 cut(s) 274, 794, 972, 1038
BssMI GATC 4 cut(s) 303, 769, 947, 1068
BssT1I CCWWGG 2 cut(s) 794, 972
Bst2UI CCWGG 1 cut(s) 683
Bst4CI ACNGT 1 cut(s) 1000
Bst6I CTCTTC 1 cut(s) 30
BstC8I GCNNGC 3 cut(s) 110, 281, 420
BstDEI CTNAG 2 cut(s) 300, 813
BstDSI CCRYGG 2 cut(s) 972, 1038
BstF5I GGATG 3 cut(s) 56, 725, 1048
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 4 cut(s) 119, 404, 472, 702
BstKTI GATC 4 cut(s) 306, 772, 950, 1071
BstMBI GATC 4 cut(s) 303, 769, 947, 1068
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 1 cut(s) 683
BstSCI CCNGG 2 cut(s) 367, 681
BstSFI CTRYAG 1 cut(s) 861
BstSLI GKGCMC 1 cut(s) 102
BstV1I GCAGC 3 cut(s) 310, 704, 817
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BstZI CGGCCG 1 cut(s) 327
BsuRI GGCC 5 cut(s) 255, 279, 329, 367, 739
BtgI CCRYGG 2 cut(s) 972, 1038
BtrI CACGTC 1 cut(s) 1017
BtsCI GGATG 3 cut(s) 56, 725, 1048
BtsI GCAGTG 2 cut(s) 89, 318
BtsIMutI CAGTG 4 cut(s) 89, 102, 159, 318
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 3 cut(s) 110, 281, 420
CaiI CAGNNNCTG 1 cut(s) 773
CfoI GCGC 4 cut(s) 119, 404, 472, 702
Cfr10I RCCGGY 2 cut(s) 480, 735
Cfr13I GGNCC 4 cut(s) 365, 566, 738, 790
Csp6I GTAC 2 cut(s) 618, 733
CspAI ACCGGT 1 cut(s) 480
CviAII CATG 4 cut(s) 10, 224, 442, 973
CviQI GTAC 2 cut(s) 618, 733
DdeI CTNAG 2 cut(s) 300, 813
DpnI GATC 4 cut(s) 305, 771, 949, 1070
DpnII GATC 4 cut(s) 303, 769, 947, 1068
DraI TTTAAA 1 cut(s) 847
EaeI YGGCCR 2 cut(s) 277, 327
EagI CGGCCG 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 316
EclXI CGGCCG 1 cut(s) 327
Eco130I CCWWGG 2 cut(s) 794, 972
Eco24I GRGCYC 1 cut(s) 318
Eco47I GGWCC 2 cut(s) 566, 790
Eco47III AGCGCT 1 cut(s) 403
Eco52I CGGCCG 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 684, 872
EcoICRI GAGCTC 1 cut(s) 316
EcoO109I RGGNCCY 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 776
EcoRII CCWGG 1 cut(s) 681
EcoT14I CCWWGG 2 cut(s) 794, 972
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 2 cut(s) 794, 972
FaeI CATG 4 cut(s) 13, 227, 445, 976
FaqI GGGAC 2 cut(s) 665, 776
FatI CATG 4 cut(s) 9, 223, 441, 972
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 2 cut(s) 531, 962
FbaI TGATCA 1 cut(s) 1068
FblI GTMKAC 1 cut(s) 864
Fnu4HI GCNGC 4 cut(s) 324, 327, 693, 831
FokI GGATG 3 cut(s) 43, 712, 1055
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 4 cut(s) 324, 327, 693, 831
FspI TGCGCA 1 cut(s) 471
GlaI GCGC 4 cut(s) 118, 403, 471, 701
GluI GCNGC 4 cut(s) 324, 327, 693, 831
GsuI CTGGAG 2 cut(s) 665, 938
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 5 cut(s) 255, 279, 329, 367, 739
HapII CCGG 3 cut(s) 368, 481, 736
HhaI GCGC 4 cut(s) 119, 404, 472, 702
Hin1II CATG 4 cut(s) 13, 227, 445, 976
Hin6I GCGC 4 cut(s) 117, 402, 470, 700
HinP1I GCGC 4 cut(s) 117, 402, 470, 700
HindIII AAGCTT 1 cut(s) 892
HinfI GANTC 2 cut(s) 296, 434
HpaII CCGG 3 cut(s) 368, 481, 736
HphI GGTGA 2 cut(s) 129, 356
Hpy166II GTNNAC 2 cut(s) 100, 865
Hpy188I TCNGA 5 cut(s) 75, 301, 664, 730, 769
Hpy188III TCNNGA 4 cut(s) 576, 773, 1020, 1072
Hpy8I GTNNAC 2 cut(s) 100, 865
HpyAV CCTTC 3 cut(s) 335, 481, 781
HpyCH4III ACNGT 1 cut(s) 1000
HpyCH4IV ACGT 1 cut(s) 1016
HpyCH4V TGCA 7 cut(s) 100, 131, 159, 410, 584, 966, 1060
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 2 cut(s) 300, 813
HpySE526I ACGT 1 cut(s) 1016
Hsp92II CATG 4 cut(s) 13, 227, 445, 976
HspAI GCGC 4 cut(s) 117, 402, 470, 700
Ksp22I TGATCA 1 cut(s) 1068
Kzo9I GATC 4 cut(s) 303, 769, 947, 1068
LmnI GCTCC 1 cut(s) 1030
Lsp1109I GCAGC 3 cut(s) 310, 704, 817
LweI GCATC 1 cut(s) 817
MaeII ACGT 1 cut(s) 1016
MalI GATC 4 cut(s) 305, 771, 949, 1070
MboI GATC 4 cut(s) 303, 769, 947, 1068
MboII GAAGA 7 cut(s) 17, 20, 23, 204, 216, 742, 889
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 3 cut(s) 102, 318, 420
MluCI AATT 9 cut(s) 244, 360, 571, 757, 776, 803, 842, 910, 1063
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 273
MroXI GAANNNNTTC 2 cut(s) 376, 669
MseI TTAA 4 cut(s) 609, 840, 846, 921
MslI CAYNNNNRTG 1 cut(s) 971
MspI CCGG 3 cut(s) 368, 481, 736
MspR9I CCNGG 2 cut(s) 369, 683
MvaI CCWGG 1 cut(s) 683
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 369
NcoI CCATGG 1 cut(s) 972
NdeI CATATG 2 cut(s) 531, 962
NdeII GATC 4 cut(s) 303, 769, 947, 1068
NlaIII CATG 4 cut(s) 13, 227, 445, 976
NlaIV GGNNCC 4 cut(s) 6, 21, 711, 792
NmeAIII GCCGAG 2 cut(s) 93, 255
NsbI TGCGCA 1 cut(s) 471
PaqCI CACCTGC 1 cut(s) 136
PdmI GAANNNNTTC 2 cut(s) 376, 669
PfeI GAWTC 1 cut(s) 434
PfoI TCCNGGA 1 cut(s) 681
PinAI ACCGGT 1 cut(s) 480
PkrI GCNGC 4 cut(s) 325, 328, 694, 832
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
PpuMI RGGWCCY 1 cut(s) 790
Psp124BI GAGCTC 1 cut(s) 318
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 1 cut(s) 681
PspGI CCWGG 1 cut(s) 681
PspN4I GGNNCC 4 cut(s) 6, 21, 711, 792
PspPI GGNCC 4 cut(s) 365, 566, 738, 790
PspPPI RGGWCCY 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 1 cut(s) 769
RsaI GTAC 2 cut(s) 619, 734
RsaNI GTAC 2 cut(s) 618, 733
RseI CAYNNNNRTG 1 cut(s) 971
SacI GAGCTC 1 cut(s) 318
SaqAI TTAA 4 cut(s) 609, 840, 846, 921
SatI GCNGC 4 cut(s) 324, 327, 693, 831
Sau3AI GATC 4 cut(s) 303, 769, 947, 1068
Sau96I GGNCC 4 cut(s) 365, 566, 738, 790
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 369, 683
SduI GDGCHC 3 cut(s) 102, 318, 420
SfaNI GCATC 1 cut(s) 817
SfcI CTRYAG 1 cut(s) 861
SinI GGWCC 2 cut(s) 566, 790
SmiMI CAYNNNNRTG 1 cut(s) 971
Sse9I AATT 9 cut(s) 244, 360, 571, 757, 776, 803, 842, 910, 1063
SsiI CCGC 3 cut(s) 47, 326, 943
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 2 cut(s) 367, 681
StyI CCWWGG 2 cut(s) 794, 972
TaaI ACNGT 1 cut(s) 1000
TaiI ACGT 1 cut(s) 1019
TaqI TCGA 2 cut(s) 66, 426
TasI AATT 9 cut(s) 244, 360, 571, 757, 776, 803, 842, 910, 1063
TatI WGTACW 1 cut(s) 617
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 1 cut(s) 434
Tru1I TTAA 4 cut(s) 609, 840, 846, 921
Tru9I TTAA 4 cut(s) 609, 840, 846, 921
TscAI CASTG 4 cut(s) 96, 102, 166, 325
TseI GCWGC 3 cut(s) 323, 692, 830
TspDTI ATGAA 3 cut(s) 26, 769, 800
TspGWI ACGGA 1 cut(s) 1055
TspRI CASTG 4 cut(s) 96, 102, 166, 325
VneI GTGCAC 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 566, 790
XapI RAATTY 4 cut(s) 571, 776, 803, 842
XmiI GTMKAC 1 cut(s) 864
XmnI GAANNNNTTC 2 cut(s) 376, 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.