RLG00000028348
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004718
Physical Location & Seq
Reverse (-)
21620543 .. 21623413
2871 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028348

Sequence Viewer

Length: 1062 bp
ATGAATTCAGCACCTCATCTTCTTCTTCAGCAATCTCTCCCGCCAATATCTTCCCTACAAAACCCAAAACCCACACTGAGACCCCCACTCCCGGCGCACAATAGCACCCCCGGCGCACGAAAATCCACACCAACTTGCATAACAGCTTGCGCCAAAACAACACGAAAAACAGGAGGAAGAAGAAAAAACCCAGATGACTACCACGGCACTCTTACAGCTCTCAACTCCAAAGGCCGCTTCCCCAGAAAATCACTCGGCCAGCATTATATGTTGGACTCAGAGATCAATGACCAGCTTGTGGCTGCGGCCGAAGTTGGGGAAGGTGATGTGGTGTTGGAAATTGGGCCGGGAACTGGTTCTTTGACTAATGTTCTTATCAATGCTGGTGCAACTGTGCTCGCAATCGAAAAGGATCCATACATGGCTACTCTTGTAAGCGAAAGATTTGCGCAGACTGGTCAGTTGAAGGTTCTACAAGAGGACTTTGTCAAATGTCATATTCATTCACATATGTCATCATTATTGAGAAGTATAGAGTCATCCGGTGCAAGTTCAAGACATGCAAAAGTAGTCGCTAATATACCCTTCAATATAAGTACAGATGTAGTCAAACAACTTCTTCCAATGGGTGACATCTTCTCTGAAGTAGTTCTCTTACTCCAGGAGGAGACAGCTTTCCGCTTGGTGGAGTCATATCTGCGGACATCTGAGTACCGGCCTATCAATGTCTTTGTCAATTTCTATTCAGATCCTGAATTTGAATTTAAGGTCCCCAGGACAAAGTTCTTTCCGCAGCCTAATGTTGATGCTGCTGTTGTTAAGTTTAGGCTGAAGCAACCTACACAGTATCCCCCAGTTTCCTCTACTAAAAGCTTCTTCTCGATGGTCAATTCAGCTTTTAATGGGAAACGCAAAATGTTGCGGAAATCACTTCAGCACATATGCACGCCCTCGGAGATTGAAAGTGCTTTGGAAAATGTTGGTCGACCTGCCACGTCAAGACCAGAAGAGCTAACCACGGATGATTTTGTGAAGTTGCATAATTTGATTGTGAAAGAGTAG

Protein Analysis

354

Amino Acids

39.06

Weight (kDa)

9.16

Isoelectric Point (pI)

62.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RrnaAD PF00398 82 - 328 2.7e-46 Ribosomal RNA adenine dimethylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 997
AccB7I CCANNNNNTGG 1 cut(s) 298
AccI GTMKAC 1 cut(s) 985
AciI CCGC 7 cut(s) 41, 235, 305, 679, 700, 791, 922
AclWI GGATC 3 cut(s) 407, 420, 743
AcoI YGGCCR 2 cut(s) 256, 306
AcsI RAATTY 3 cut(s) 4, 755, 761
AcuI CTGAAG 4 cut(s) 11, 663, 851, 917
AfaI GTAC 2 cut(s) 598, 713
AfiI CCNNNNNNNGG 5 cut(s) 91, 298, 315, 353, 685
AgsI TTSAA 5 cut(s) 466, 555, 589, 761, 962
AjiI CACGTC 1 cut(s) 996
AjnI CCWGG 2 cut(s) 660, 773
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 7 cut(s) 146, 218, 295, 674, 873, 896, 1012
AluI AGCT 7 cut(s) 146, 218, 295, 674, 873, 896, 1012
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 2 cut(s) 73, 662
AlwI GGATC 3 cut(s) 407, 420, 743
AlwNI CAGNNNCTG 1 cut(s) 752
AoxI GGCC 5 cut(s) 232, 256, 306, 344, 716
ApeKI GCWGC 3 cut(s) 302, 793, 809
ApoI RAATTY 3 cut(s) 4, 755, 761
Asp700I GAANNNNTTC 2 cut(s) 355, 648
AspLEI GCGC 4 cut(s) 97, 116, 152, 451
AspS9I GGNCC 2 cut(s) 344, 769
AsuC2I CCSGG 3 cut(s) 92, 111, 348
AsuHPI GGTGA 2 cut(s) 335, 641
AvaII GGWCC 1 cut(s) 769
BamHI GGATCC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 3 cut(s) 289, 796, 805
BccI CCATC 1 cut(s) 877
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 2 cut(s) 662, 775
BciVI GTATCC 1 cut(s) 858
BcnI CCSGG 3 cut(s) 92, 111, 348
BcoDI GTCTC 2 cut(s) 73, 662
BfuAI ACCTGC 1 cut(s) 997
BfuI GTATCC 1 cut(s) 858
BisI GCNGC 5 cut(s) 235, 303, 306, 794, 810
BlsI GCNGC 5 cut(s) 236, 304, 307, 795, 811
Bme1390I CCNGG 5 cut(s) 92, 111, 348, 662, 775
Bme18I GGWCC 1 cut(s) 769
BmgBI CACGTC 1 cut(s) 996
BmgT120I GGNCC 2 cut(s) 344, 769
BmiI GGNNCC 2 cut(s) 414, 771
BmrFI CCNGG 5 cut(s) 92, 111, 348, 662, 775
BmrI ACTGGG 1 cut(s) 848
BmsI GCATC 1 cut(s) 796
BmuI ACTGGG 1 cut(s) 848
BpmI CTGGAG 1 cut(s) 644
BpuMI CCSGG 3 cut(s) 92, 111, 348
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 5 cut(s) 109, 202, 773, 951, 1017
BsaWI WCCGGW 1 cut(s) 542
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 298, 315, 353, 685
Bse118I RCCGGY 1 cut(s) 714
Bse1I ACTGG 3 cut(s) 358, 460, 854
BseBI CCWGG 2 cut(s) 662, 775
BseDI CCNNGG 5 cut(s) 109, 202, 773, 951, 1017
BseGI GGATG 2 cut(s) 539, 1027
BseLI CCNNNNNNNGG 5 cut(s) 91, 298, 315, 353, 685
BseMII CTCAG 3 cut(s) 68, 291, 699
BseNI ACTGG 3 cut(s) 358, 460, 854
BseRI GAGGAG 1 cut(s) 680
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 3 cut(s) 289, 796, 805
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 5 cut(s) 234, 258, 308, 346, 718
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 5 cut(s) 92, 111, 347, 543, 715
BslFI GGGAC 1 cut(s) 755
BslI CCNNNNNNNGG 5 cut(s) 91, 298, 315, 353, 685
BsmAI GTCTC 2 cut(s) 73, 662
BsmFI GGGAC 1 cut(s) 755
BsnI GGCC 5 cut(s) 234, 258, 308, 346, 718
Bso31I GGTCTC 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 3 cut(s) 282, 412, 748
BspACI CCGC 7 cut(s) 41, 235, 305, 679, 700, 791, 922
BspANI GGCC 5 cut(s) 234, 258, 308, 346, 718
BspCNI CTCAG 3 cut(s) 69, 290, 700
BspLI GGNNCC 2 cut(s) 414, 771
BspMI ACCTGC 1 cut(s) 997
BspPI GGATC 3 cut(s) 407, 420, 743
BspQI GCTCTTC 1 cut(s) 1002
BspTNI GGTCTC 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 714
BsrI ACTGG 3 cut(s) 358, 460, 854
BssAI RCCGGY 1 cut(s) 714
BssECI CCNNGG 5 cut(s) 109, 202, 773, 951, 1017
BssMI GATC 3 cut(s) 282, 412, 748
Bst2UI CCWGG 2 cut(s) 662, 775
Bst4CI ACNGT 2 cut(s) 394, 846
Bst6I CTCTTC 1 cut(s) 1002
BstC8I GCNNGC 4 cut(s) 148, 260, 399, 947
BstDEI CTNAG 3 cut(s) 77, 277, 708
BstDSI CCRYGG 2 cut(s) 202, 1017
BstF5I GGATG 2 cut(s) 539, 1027
BstHHI GCGC 4 cut(s) 97, 116, 152, 451
BstKTI GATC 3 cut(s) 285, 415, 751
BstMAI GTCTC 2 cut(s) 73, 662
BstMBI GATC 3 cut(s) 282, 412, 748
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstNI CCWGG 2 cut(s) 662, 775
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 5 cut(s) 90, 109, 346, 660, 773
BstV1I GCAGC 3 cut(s) 289, 796, 805
BstX2I RGATCY 2 cut(s) 412, 748
BstYI RGATCY 2 cut(s) 412, 748
BstZI CGGCCG 1 cut(s) 306
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 5 cut(s) 234, 258, 308, 346, 718
BtgI CCRYGG 2 cut(s) 202, 1017
BtrI CACGTC 1 cut(s) 996
BtsCI GGATG 2 cut(s) 539, 1027
BtsIMutI CAGTG 1 cut(s) 74
BveI ACCTGC 1 cut(s) 997
Cac8I GCNNGC 4 cut(s) 148, 260, 399, 947
CaiI CAGNNNCTG 1 cut(s) 752
CfoI GCGC 4 cut(s) 97, 116, 152, 451
Cfr10I RCCGGY 1 cut(s) 714
Cfr13I GGNCC 2 cut(s) 344, 769
Csp6I GTAC 2 cut(s) 597, 712
CviAII CATG 2 cut(s) 421, 560
CviQI GTAC 2 cut(s) 597, 712
DdeI CTNAG 3 cut(s) 77, 277, 708
DpnI GATC 3 cut(s) 284, 414, 750
DpnII GATC 3 cut(s) 282, 412, 748
EaeI YGGCCR 2 cut(s) 256, 306
EagI CGGCCG 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
EclXI CGGCCG 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 769
Eco52I CGGCCG 1 cut(s) 306
Eco57I CTGAAG 4 cut(s) 11, 663, 851, 917
EcoO109I RGGNCCY 1 cut(s) 769
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 2 cut(s) 660, 773
FaeI CATG 2 cut(s) 424, 563
FaqI GGGAC 1 cut(s) 755
FatI CATG 2 cut(s) 420, 559
FauI CCCGC 1 cut(s) 48
FauNDI CATATG 2 cut(s) 510, 941
FblI GTMKAC 1 cut(s) 985
Fnu4HI GCNGC 5 cut(s) 235, 303, 306, 794, 810
FokI GGATG 2 cut(s) 526, 1034
Fsp4HI GCNGC 5 cut(s) 235, 303, 306, 794, 810
FspI TGCGCA 1 cut(s) 450
GlaI GCGC 4 cut(s) 96, 115, 151, 450
GluI GCNGC 5 cut(s) 235, 303, 306, 794, 810
GsuI CTGGAG 1 cut(s) 644
HaeIII GGCC 5 cut(s) 234, 258, 308, 346, 718
HapII CCGG 5 cut(s) 92, 111, 347, 543, 715
HhaI GCGC 4 cut(s) 97, 116, 152, 451
Hin1II CATG 2 cut(s) 424, 563
Hin6I GCGC 4 cut(s) 95, 114, 150, 449
HinP1I GCGC 4 cut(s) 95, 114, 150, 449
HincII GTYRAC 1 cut(s) 986
HindII GTYRAC 1 cut(s) 986
HindIII AAGCTT 1 cut(s) 871
HinfI GANTC 3 cut(s) 275, 536, 689
HpaII CCGG 5 cut(s) 92, 111, 347, 543, 715
HphI GGTGA 2 cut(s) 335, 641
Hpy166II GTNNAC 1 cut(s) 986
Hpy188I TCNGA 5 cut(s) 280, 643, 709, 748, 955
Hpy188III TCNNGA 4 cut(s) 555, 752, 880, 999
Hpy8I GTNNAC 1 cut(s) 986
HpyAV CCTTC 3 cut(s) 314, 460, 595
HpyCH4III ACNGT 2 cut(s) 394, 846
HpyCH4IV ACGT 1 cut(s) 995
HpyCH4V TGCA 6 cut(s) 138, 389, 548, 563, 945, 1039
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 3 cut(s) 77, 277, 708
HpySE526I ACGT 1 cut(s) 995
Hsp92II CATG 2 cut(s) 424, 563
HspAI GCGC 4 cut(s) 95, 114, 150, 449
Kzo9I GATC 3 cut(s) 282, 412, 748
LguI GCTCTTC 1 cut(s) 1002
Lsp1109I GCAGC 3 cut(s) 289, 796, 805
LweI GCATC 1 cut(s) 796
MaeII ACGT 1 cut(s) 995
MaeIII GTNAC 1 cut(s) 629
MalI GATC 3 cut(s) 284, 414, 750
MboI GATC 3 cut(s) 282, 412, 748
MflI RGATCY 2 cut(s) 412, 748
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 7 cut(s) 4, 339, 736, 755, 761, 889, 1042
MlyI GAGTC 3 cut(s) 269, 545, 698
MmeI TCCRAC 2 cut(s) 252, 315
MnlI CCTC 6 cut(s) 24, 167, 472, 658, 871, 961
MroXI GAANNNNTTC 2 cut(s) 355, 648
MseI TTAA 3 cut(s) 765, 819, 900
MspI CCGG 5 cut(s) 92, 111, 347, 543, 715
MspR9I CCNGG 5 cut(s) 92, 111, 348, 662, 775
MvaI CCWGG 2 cut(s) 662, 775
MwoI GCNNNNNNNGC 1 cut(s) 111
NciI CCSGG 3 cut(s) 92, 111, 348
NdeI CATATG 2 cut(s) 510, 941
NdeII GATC 3 cut(s) 282, 412, 748
NlaIII CATG 2 cut(s) 424, 563
NlaIV GGNNCC 2 cut(s) 414, 771
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 629
NsbI TGCGCA 1 cut(s) 450
NspI RCATGY 1 cut(s) 563
PciSI GCTCTTC 1 cut(s) 1002
PdmI GAANNNNTTC 2 cut(s) 355, 648
PflFI GACNNNGTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 298
PfoI TCCNGGA 1 cut(s) 660
PkrI GCNGC 5 cut(s) 236, 304, 307, 795, 811
PleI GAGTC 3 cut(s) 269, 544, 697
PpsI GAGTC 3 cut(s) 269, 544, 697
PpuMI RGGWCCY 1 cut(s) 769
Psp5II RGGWCCY 1 cut(s) 769
Psp6I CCWGG 2 cut(s) 660, 773
PspGI CCWGG 2 cut(s) 660, 773
PspN4I GGNNCC 2 cut(s) 414, 771
PspPI GGNCC 2 cut(s) 344, 769
PspPPI RGGWCCY 1 cut(s) 769
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 2 cut(s) 412, 748
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 2 cut(s) 598, 713
RsaNI GTAC 2 cut(s) 597, 712
SalI GTCGAC 1 cut(s) 984
SapI GCTCTTC 1 cut(s) 1002
SaqAI TTAA 3 cut(s) 765, 819, 900
SatI GCNGC 5 cut(s) 235, 303, 306, 794, 810
Sau3AI GATC 3 cut(s) 282, 412, 748
Sau96I GGNCC 2 cut(s) 344, 769
SchI GAGTC 3 cut(s) 269, 545, 698
ScrFI CCNGG 5 cut(s) 92, 111, 348, 662, 775
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 1 cut(s) 796
SinI GGWCC 1 cut(s) 769
Sse9I AATT 7 cut(s) 4, 339, 736, 755, 761, 889, 1042
SsiI CCGC 7 cut(s) 41, 235, 305, 679, 700, 791, 922
StyD4I CCNGG 5 cut(s) 90, 109, 346, 660, 773
TaaI ACNGT 2 cut(s) 394, 846
TaiI ACGT 1 cut(s) 998
TaqI TCGA 3 cut(s) 405, 881, 985
TasI AATT 7 cut(s) 4, 339, 736, 755, 761, 889, 1042
TatI WGTACW 1 cut(s) 596
TauI GCSGC 2 cut(s) 237, 308
Tru1I TTAA 3 cut(s) 765, 819, 900
Tru9I TTAA 3 cut(s) 765, 819, 900
TscAI CASTG 1 cut(s) 81
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 3 cut(s) 302, 793, 809
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 2 cut(s) 17, 491
TspGWI ACGGA 1 cut(s) 1034
TspRI CASTG 1 cut(s) 81
Tth111I GACNNNGTC 1 cut(s) 485
Van91I CCANNNNNTGG 1 cut(s) 298
VpaK11BI GGWCC 1 cut(s) 769
XapI RAATTY 3 cut(s) 4, 755, 761
XceI RCATGY 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 985
XmnI GAANNNNTTC 2 cut(s) 355, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.