Rh1AG240300
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004718
Physical Location & Seq
Forward (+)
45111403 .. 45114223
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG240300.1

Sequence Viewer

Length: 1065 bp
ATGAATTCAGCACCTCATCTTCTTCTTCTTCAGCAATCTCTCCCGCCAATATCTTCCCTACAAAACCCAAAACCCACACTGAGACCCTCACTCCCGGCGCATAATAGCACCCCCGGCGCACGAAAATCCACACCAACTTACATAACAGCTTGCGCCAAAACAACACAAAAAACAGGAGGAAGAAGAAAAAACCCTGATGACTACCACGCCACTCTTACAGCTCTCAACTCCAAAGGCCGCTTCCCCAGAAAATCACTCGGCCAGCATTATATGTTGGACTCAGAGATCAATGACCACCTTGTGGCTGCGGCGGAAGTTGGGGAAGGTGATGCGGTGTTGGAAATTGGGCCGGGAACTGGTTCTTTGACTAATGTTCTTATCAATGCTGGTGCAACTGTGCTCGCAATCGAAAAGGATCCATACATGGCTACTCTTGTAAGCGAAAGATTTGCGCAGACTGGTCAGTTGAAGGTTCTACAAGAGGACTTTGTCAAATGTCATATTCATTCACACATGTCATCATTATTGAGAAGTATAGAGTCATCCGGTGCAAGTTCAAGACATGCAAAAGTAGTCGCTAATATACCCTTTAATATAAGTACAGATGTAGTCAAACAACTTCTACCAATGGGTGACATCTTCTCTGAAGTAGTTCTCTTACTCCAGGAGGAGACAGCTTTCCGCTTGGTGGAGTCATATCTGCGGACATCTGAGTACCGGCCTATCAATGTCTTTGTCAATTTCTATTCAGATCCTGAATTTAAATTTAAGGTCCCCAGGACAAACTTCTTTCCTCAGCCTAATGTTGATGCTGCTGTTGTTAAGTTTAGGCTGAAGCAACCTACACAGTATCCCCCAGTTTCCTCTACTAAAAGCTTCTTCTCAATGGTCAATTCAGCTTTTAATGGGAAACGCAAAATGTTGCGGAAATCACTTCAGCACATATGCACGCCCTCGGAGATTGAAAGTGCTTTGGAAAATGTTGGTCGACCTGCCACGTCAAGACCAGAAGAGCTAACCACGGATGATTTTGTGAAGTTGCATAATTTGATTGTGAAAGAGTAG

Protein Analysis

354

Amino Acids

39.17

Weight (kDa)

9.2

Isoelectric Point (pI)

61.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RrnaAD PF00398 83 - 329 4.9e-46 Ribosomal RNA adenine dimethylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 453
Acc36I ACCTGC 1 cut(s) 1000
AccB7I CCANNNNNTGG 1 cut(s) 301
AccI GTMKAC 1 cut(s) 988
AciI CCGC 8 cut(s) 44, 238, 308, 311, 332, 682, 703, 925
AclWI GGATC 3 cut(s) 410, 423, 746
AcoI YGGCCR 1 cut(s) 259
AcsI RAATTY 3 cut(s) 4, 758, 764
AcuI CTGAAG 4 cut(s) 14, 666, 854, 920
AdeI CACNNNGTG 1 cut(s) 301
AfaI GTAC 2 cut(s) 601, 716
AfiI CCNNNNNNNGG 3 cut(s) 301, 356, 688
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 3 cut(s) 469, 558, 965
AjiI CACGTC 1 cut(s) 999
AjnI CCWGG 2 cut(s) 663, 776
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 6 cut(s) 149, 221, 677, 876, 899, 1015
AluI AGCT 6 cut(s) 149, 221, 677, 876, 899, 1015
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 2 cut(s) 76, 665
AlwI GGATC 3 cut(s) 410, 423, 746
AlwNI CAGNNNCTG 1 cut(s) 755
AoxI GGCC 4 cut(s) 235, 259, 347, 719
ApeKI GCWGC 2 cut(s) 305, 812
ApoI RAATTY 3 cut(s) 4, 758, 764
Asp700I GAANNNNTTC 2 cut(s) 358, 651
AspLEI GCGC 4 cut(s) 100, 119, 155, 454
AspS9I GGNCC 2 cut(s) 347, 772
AsuC2I CCSGG 3 cut(s) 95, 114, 351
AsuHPI GGTGA 2 cut(s) 338, 644
AvaII GGWCC 1 cut(s) 772
BamHI GGATCC 1 cut(s) 415
Bbv12I GWGCWC 1 cut(s) 402
BbvCI CCTCAGC 1 cut(s) 795
BbvI GCAGC 2 cut(s) 292, 799
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciT130I CCWGG 2 cut(s) 665, 778
BciVI GTATCC 1 cut(s) 861
BcnI CCSGG 3 cut(s) 95, 114, 351
BcoDI GTCTC 2 cut(s) 76, 665
BfuAI ACCTGC 1 cut(s) 1000
BfuI GTATCC 1 cut(s) 861
BisI GCNGC 4 cut(s) 238, 306, 309, 813
BlsI GCNGC 4 cut(s) 239, 307, 310, 814
Bme1390I CCNGG 5 cut(s) 95, 114, 351, 665, 778
Bme18I GGWCC 1 cut(s) 772
BmgBI CACGTC 1 cut(s) 999
BmgT120I GGNCC 2 cut(s) 347, 772
BmiI GGNNCC 2 cut(s) 417, 774
BmrFI CCNGG 5 cut(s) 95, 114, 351, 665, 778
BmrI ACTGGG 1 cut(s) 851
BmsI GCATC 2 cut(s) 319, 799
BmuI ACTGGG 1 cut(s) 851
BpmI CTGGAG 1 cut(s) 647
Bpu10I CCTNAGC 1 cut(s) 795
BpuMI CCSGG 3 cut(s) 95, 114, 351
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 4 cut(s) 112, 776, 954, 1020
BsaWI WCCGGW 1 cut(s) 545
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 356, 688
Bse118I RCCGGY 1 cut(s) 717
Bse1I ACTGG 3 cut(s) 361, 463, 857
BseBI CCWGG 2 cut(s) 665, 778
BseDI CCNNGG 4 cut(s) 112, 776, 954, 1020
BseGI GGATG 2 cut(s) 542, 1030
BseLI CCNNNNNNNGG 3 cut(s) 301, 356, 688
BseMII CTCAG 4 cut(s) 71, 294, 702, 809
BseNI ACTGG 3 cut(s) 361, 463, 857
BseRI GAGGAG 1 cut(s) 683
BseXI GCAGC 2 cut(s) 292, 799
BshFI GGCC 4 cut(s) 237, 261, 349, 721
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 5 cut(s) 95, 114, 350, 546, 718
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 3 cut(s) 301, 356, 688
BsmAI GTCTC 2 cut(s) 76, 665
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 4 cut(s) 237, 261, 349, 721
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 3 cut(s) 285, 415, 751
BspACI CCGC 8 cut(s) 44, 238, 308, 311, 332, 682, 703, 925
BspANI GGCC 4 cut(s) 237, 261, 349, 721
BspCNI CTCAG 4 cut(s) 72, 293, 703, 808
BspLI GGNNCC 2 cut(s) 417, 774
BspMI ACCTGC 1 cut(s) 1000
BspPI GGATC 3 cut(s) 410, 423, 746
BspQI GCTCTTC 1 cut(s) 1005
BspTNI GGTCTC 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 717
BsrI ACTGG 3 cut(s) 361, 463, 857
BssAI RCCGGY 1 cut(s) 717
BssECI CCNNGG 4 cut(s) 112, 776, 954, 1020
BssMI GATC 3 cut(s) 285, 415, 751
Bst2UI CCWGG 2 cut(s) 665, 778
Bst4CI ACNGT 2 cut(s) 397, 849
Bst6I CTCTTC 1 cut(s) 1005
BstC8I GCNNGC 4 cut(s) 151, 263, 402, 950
BstDEI CTNAG 4 cut(s) 80, 280, 711, 795
BstDSI CCRYGG 1 cut(s) 1020
BstF5I GGATG 2 cut(s) 542, 1030
BstHHI GCGC 4 cut(s) 100, 119, 155, 454
BstKTI GATC 3 cut(s) 288, 418, 754
BstMAI GTCTC 2 cut(s) 76, 665
BstMBI GATC 3 cut(s) 285, 415, 751
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 2 cut(s) 665, 778
BstNSI RCATGY 2 cut(s) 517, 566
BstSCI CCNGG 5 cut(s) 93, 112, 349, 663, 776
BstV1I GCAGC 2 cut(s) 292, 799
BstX2I RGATCY 2 cut(s) 415, 751
BstYI RGATCY 2 cut(s) 415, 751
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 4 cut(s) 237, 261, 349, 721
BtgI CCRYGG 1 cut(s) 1020
BtrI CACGTC 1 cut(s) 999
BtsCI GGATG 2 cut(s) 542, 1030
BtsIMutI CAGTG 1 cut(s) 77
BveI ACCTGC 1 cut(s) 1000
Cac8I GCNNGC 4 cut(s) 151, 263, 402, 950
CaiI CAGNNNCTG 1 cut(s) 755
CfoI GCGC 4 cut(s) 100, 119, 155, 454
Cfr10I RCCGGY 1 cut(s) 717
Cfr13I GGNCC 2 cut(s) 347, 772
Csp6I GTAC 2 cut(s) 600, 715
CviAII CATG 3 cut(s) 424, 514, 563
CviQI GTAC 2 cut(s) 600, 715
DdeI CTNAG 4 cut(s) 80, 280, 711, 795
DpnI GATC 3 cut(s) 287, 417, 753
DpnII GATC 3 cut(s) 285, 415, 751
DraI TTTAAA 1 cut(s) 763
DraIII CACNNNGTG 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 1 cut(s) 1005
EarI CTCTTC 1 cut(s) 1005
EciI GGCGGA 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 772
Eco57I CTGAAG 4 cut(s) 14, 666, 854, 920
EcoO109I RGGNCCY 1 cut(s) 772
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 2 cut(s) 663, 776
FaeI CATG 3 cut(s) 427, 517, 566
FaqI GGGAC 1 cut(s) 758
FatI CATG 3 cut(s) 423, 513, 562
FauI CCCGC 1 cut(s) 51
FauNDI CATATG 1 cut(s) 944
FblI GTMKAC 1 cut(s) 988
Fnu4HI GCNGC 4 cut(s) 238, 306, 309, 813
FokI GGATG 2 cut(s) 529, 1037
Fsp4HI GCNGC 4 cut(s) 238, 306, 309, 813
FspI TGCGCA 1 cut(s) 453
GlaI GCGC 4 cut(s) 99, 118, 154, 453
GluI GCNGC 4 cut(s) 238, 306, 309, 813
GsuI CTGGAG 1 cut(s) 647
HaeIII GGCC 4 cut(s) 237, 261, 349, 721
HapII CCGG 5 cut(s) 95, 114, 350, 546, 718
HhaI GCGC 4 cut(s) 100, 119, 155, 454
Hin1II CATG 3 cut(s) 427, 517, 566
Hin6I GCGC 4 cut(s) 98, 117, 153, 452
HinP1I GCGC 4 cut(s) 98, 117, 153, 452
HincII GTYRAC 1 cut(s) 989
HindII GTYRAC 1 cut(s) 989
HindIII AAGCTT 1 cut(s) 874
HinfI GANTC 3 cut(s) 278, 539, 692
HpaII CCGG 5 cut(s) 95, 114, 350, 546, 718
HphI GGTGA 2 cut(s) 338, 644
Hpy166II GTNNAC 1 cut(s) 989
Hpy188I TCNGA 5 cut(s) 283, 646, 712, 751, 958
Hpy188III TCNNGA 3 cut(s) 558, 755, 1002
Hpy8I GTNNAC 1 cut(s) 989
HpyAV CCTTC 2 cut(s) 317, 463
HpyCH4III ACNGT 2 cut(s) 397, 849
HpyCH4IV ACGT 1 cut(s) 998
HpyCH4V TGCA 5 cut(s) 392, 551, 566, 948, 1042
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 4 cut(s) 80, 280, 711, 795
HpySE526I ACGT 1 cut(s) 998
Hsp92II CATG 3 cut(s) 427, 517, 566
HspAI GCGC 4 cut(s) 98, 117, 153, 452
Kzo9I GATC 3 cut(s) 285, 415, 751
LguI GCTCTTC 1 cut(s) 1005
Lsp1109I GCAGC 2 cut(s) 292, 799
LweI GCATC 2 cut(s) 319, 799
MaeII ACGT 1 cut(s) 998
MaeIII GTNAC 1 cut(s) 632
MalI GATC 3 cut(s) 287, 417, 753
MboI GATC 3 cut(s) 285, 415, 751
MflI RGATCY 2 cut(s) 415, 751
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 7 cut(s) 4, 342, 739, 758, 764, 892, 1045
MlyI GAGTC 3 cut(s) 272, 548, 701
MmeI TCCRAC 2 cut(s) 255, 318
MnlI CCTC 8 cut(s) 24, 97, 170, 475, 661, 804, 874, 964
MroXI GAANNNNTTC 2 cut(s) 358, 651
MseI TTAA 5 cut(s) 591, 762, 768, 822, 903
MspI CCGG 5 cut(s) 95, 114, 350, 546, 718
MspR9I CCNGG 5 cut(s) 95, 114, 351, 665, 778
MvaI CCWGG 2 cut(s) 665, 778
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 3 cut(s) 95, 114, 351
NdeI CATATG 1 cut(s) 944
NdeII GATC 3 cut(s) 285, 415, 751
NlaIII CATG 3 cut(s) 427, 517, 566
NlaIV GGNNCC 2 cut(s) 417, 774
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 632
NsbI TGCGCA 1 cut(s) 453
NspI RCATGY 2 cut(s) 517, 566
PciI ACATGT 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 1005
PdmI GAANNNNTTC 2 cut(s) 358, 651
PflFI GACNNNGTC 1 cut(s) 488
PflMI CCANNNNNTGG 1 cut(s) 301
PfoI TCCNGGA 1 cut(s) 663
PkrI GCNGC 4 cut(s) 239, 307, 310, 814
PleI GAGTC 3 cut(s) 272, 547, 700
PpsI GAGTC 3 cut(s) 272, 547, 700
PpuMI RGGWCCY 1 cut(s) 772
PscI ACATGT 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 772
Psp6I CCWGG 2 cut(s) 663, 776
PspGI CCWGG 2 cut(s) 663, 776
PspN4I GGNNCC 2 cut(s) 417, 774
PspPI GGNCC 2 cut(s) 347, 772
PspPPI RGGWCCY 1 cut(s) 772
PstNI CAGNNNCTG 1 cut(s) 755
PsuI RGATCY 2 cut(s) 415, 751
PsyI GACNNNGTC 1 cut(s) 488
RsaI GTAC 2 cut(s) 601, 716
RsaNI GTAC 2 cut(s) 600, 715
SalI GTCGAC 1 cut(s) 987
SapI GCTCTTC 1 cut(s) 1005
SaqAI TTAA 5 cut(s) 591, 762, 768, 822, 903
SatI GCNGC 4 cut(s) 238, 306, 309, 813
Sau3AI GATC 3 cut(s) 285, 415, 751
Sau96I GGNCC 2 cut(s) 347, 772
SchI GAGTC 3 cut(s) 272, 548, 701
ScrFI CCNGG 5 cut(s) 95, 114, 351, 665, 778
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 2 cut(s) 319, 799
SinI GGWCC 1 cut(s) 772
SmiI ATTTAAAT 1 cut(s) 763
Sse9I AATT 7 cut(s) 4, 342, 739, 758, 764, 892, 1045
SsiI CCGC 8 cut(s) 44, 238, 308, 311, 332, 682, 703, 925
StyD4I CCNGG 5 cut(s) 93, 112, 349, 663, 776
SwaI ATTTAAAT 1 cut(s) 763
TaaI ACNGT 2 cut(s) 397, 849
TaiI ACGT 1 cut(s) 1001
TaqI TCGA 2 cut(s) 408, 988
TasI AATT 7 cut(s) 4, 342, 739, 758, 764, 892, 1045
TatI WGTACW 1 cut(s) 599
TauI GCSGC 2 cut(s) 240, 311
Tru1I TTAA 5 cut(s) 591, 762, 768, 822, 903
Tru9I TTAA 5 cut(s) 591, 762, 768, 822, 903
TscAI CASTG 1 cut(s) 84
TseFI GTSAC 1 cut(s) 632
TseI GCWGC 2 cut(s) 305, 812
Tsp45I GTSAC 1 cut(s) 632
TspDTI ATGAA 2 cut(s) 17, 494
TspGWI ACGGA 1 cut(s) 1037
TspRI CASTG 1 cut(s) 84
Tth111I GACNNNGTC 1 cut(s) 488
Van91I CCANNNNNTGG 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 772
XapI RAATTY 3 cut(s) 4, 758, 764
XceI RCATGY 2 cut(s) 517, 566
XmiI GTMKAC 1 cut(s) 988
XmnI GAANNNNTTC 2 cut(s) 358, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.