Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002240

Physical Location & Seq

Genomic Coordinates Reverse (-)

23311213 .. 23315190

Gene Structure 3978 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1159300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1848 bp

ATGGCAACCATCACTGCTTCAAGCTTTGCCTCCACAAGTTCCCATGTCAGCTATGGCGCTGTATCGTCCGGATCAGACCCGAAAACAGGATTTAAGAAAATGAGTTTTGGAAAACAGACCCTGACTCACAATGGGTTGAGAGCTTTGAACTCGGTGGACGAGCTCCGTGTCAGAATCATGGCCAATTCAGTTGCAAGGCAAACGAGGGGCAAAACCGTCAACAGCACTAGGAAGACTTCGGGAGTCATTGTTTGTGGAAGTGGGATGAATTTAGTGTTTCTGGGAACTGAGGTTGGTCCATGGAGTAAAACTGGTGGACTTGGTGATGTTCTTGGAGGCCTCCCACCGGCAATGGCTGCAAATGGGCATCGAGTGATGACGATTTCTCCGCGTTATGATCAGTACAAGGATGCATGGGATACAGAAGTCACAGTTGAGCTTAAAGTGGGAGATAAAACCGAAACAGTTCGATTTTTTCACTGCTACAAACGAGGAGTTGATCGTGTTTTCGTGGATCACCCATTGTTTCTCGAAAAGGTATGGGGGAAAACTGCATCCAAAATTTATGGTCCGGTCGCTGGAGTCGATTTCAAGGACAATCAGCTTAGATTCAGCCTTCTATGCCAGGCAGCTCTAGTAGCACCAAGAGTTCTGAACCTTAACAGCAGCAAATATTTTTCTGGACCATATGGGGAAGAAGTTGTTTTCATTGCCAACGATTGGCACACTGCCCTGCTTCCATGCTACCTGAAAGCCATATACAAGCCTAAAGGCATATACAAGACCGCCAAAGTAGCCTTTTGCATTCACAACATTGCTTACCAAGGCAGATTTGCTTTCGCTGACTTTGCACTTCTCAATCTGCCCAATGAATTCAAGAGCTCGTTTGATTTCATTGACGGGTATAACAAACCAGTAAAGGGGAGGAAAATCAACTGGATGAAAGCAGGAATCTTGGAATCAGACAAGGTCTTGACTGTTAGCCCATACTATGCCGAAGAACTTGTTTCTTCAGTTGAGAAAGGAGTTGAATTGGATAACATTCTTCGGAAATCTAGAATTCAAGGAATTGTGAATGGCATGGACGTCCAGGAATGGAATCCCGTAACTGACAAGTATACAACTGTCAAATATGATGCTTCAACTGTGGCTGATGCAAAGCCTCTTTTGAAAGAAGCCCTCCAAGCAGAAGTCGGACTGCCGGTGGATAGAGACATCCCTGTCATAGGTTTCATTGGGAGGCTTGAAGAGCAGAAGGGTTCGGACATTCTCATAGAAGCCATTCCCCATTTTATCAAAGAGAATGTTCAGATTATAGTCCTCGGGACTGGCAAAAAGCCAATGGAGAAGCAGCTTGAACAGCTGGAAACAGAATACCCAGACAAAGCCCGAGGAATAGCGAAATTCAATGTCCCTCTGGCCCATATGATCACGGCAGGAGCTGATTTCATGTTGGTTCCAAGCAGATTCGAGCCATGTGGTCTCATCCAGTTGCACGCCATGCGTTATGGAACTGTGCCCATTGTTGCGTCAACCGGCGGGTTGGTAGACACTGTTAAAGAAGGATTCACTGGATTTCATATGGGAGCCTTCAATGTGGAATGCGAAGTTGTTGATCCAGTGGATGTACAAGCAATTGCTACAACCGTCACAAGAGCCCTCGGAAGTTATGGAACTCCAGCTTTTACTGAGATCATAAGCAACTGCATGGCTCAAGATCTCTCGTGGAAGGGTCCTGCTAAGAAGTGGGAGGAAGTGCTGCTGAGTTTGGGTGTCGCCAACAGCGAACTTGGGATTGAGGGTGAGGAAATTGCACCGCTCGCAAAGGAAAATGTCGCAACGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000271 GO:0003674 GO:0003824 GO:0004373 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0008194 GO:0009011 GO:0009058 GO:0009059 GO:0009250 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0017076 GO:0019252 GO:0019863 GO:0019865 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033692 GO:0034637 GO:0034645 GO:0035251 GO:0036094 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043531 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0046527 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Glyco_transf_5 Glycos_transf_1

Protein Analysis

616

Amino Acids

67.25

Weight (kDa)

7.57

Isoelectric Point (pI)

25.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_5	PF08323	91 - 351	2.6e-62	Starch synthase catalytic domain
GT4-conflict	PF20706	386 - 579	4.7e-07	Family 4 Glycosyltransferase in conflict systems
Glycos_transf_1	PF00534	405 - 532	3.3e-18	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	407 - 554	1.5e-16	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002240)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32900
fragaria_vesca	FvH4_5g10580 FvH4_5g10580 FvH4_5g10580 FvH4_5g10580 FvH4_5g10580 FvH4_5g10580 FvH4_7g17630 FvH4_7g17630 FvH4_7g17630 FvH4_7g17630
malus_domestica	MD06G1122100.v1.1 MD07G1159300.v1.1 MD14G1138500.v1.1
prunus_persica	Prupe.2G197400_v2.0.a1 Prupe.2G197400_v2.0.a1 Prupe.2G197400_v2.0.a1 Prupe.5G132800_v2.0.a1 Prupe.5G132800_v2.0.a1
pyrus_communis	pycom01g11240 pycom06g11320 pycom07g15590 pycom14g11360
rosa_chinensis	RchiOBHm_Chr1g0361061 RchiOBHm_Chr7g0187571
rosa_laevigata	RLG00000004770 RLG00000027751
rosa_multiflora	Rmu_co8027448.1_g000001 Rmu_sc0000677.1_g000001 Rmu_ssc0000110.1_g000020
rosa_roxburghii	Rroxscaffold_3G00267310 Rroxscaffold_4G00295460
rosa_rugosa	Rorug01G0285200 Rorug06G0482700
rosa_samantha	Rh1AG296600 Rh1BG260900 Rh1CG278200 Rh1DG290900 Rh7AG087600 Rh7BG088900 Rh7CG088100 Rh7DG089800
rosa_wichuraiana	Rw1G026200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1220
AatII	GACGTC	1 cut(s)	1089
AccB7I	CCANNNNNTGG	1 cut(s)	720
AccBSI	CCGCTC	1 cut(s)	1819
AccI	GTMKAC	2 cut(s)	1118, 1548
AccII	CGCG	1 cut(s)	391
AccIII	TCCGGA	1 cut(s)	68
AciI	CCGC	4 cut(s)	389, 786, 1539, 1817
AclWI	GGATC	3 cut(s)	79, 522, 1610
AcoI	YGGCCR	1 cut(s)	180
AcsI	RAATTY	5 cut(s)	268, 561, 872, 1059, 1403
AcuI	CTGAAG	1 cut(s)	996
AcyI	GRCGYC	1 cut(s)	1086
AfaI	GTAC	2 cut(s)	404, 1629
AfiI	CCNNNNNNNGG	6 cut(s)	86, 346, 578, 720, 920, 1226
AjnI	CCWGG	2 cut(s)	624, 1089
Alw21I	GWGCWC	2 cut(s)	165, 884
Alw26I	GTCTC	2 cut(s)	1206, 1487
AlwI	GGATC	3 cut(s)	79, 522, 1610
AlwNI	CAGNNNCTG	2 cut(s)	121, 1442
Ama87I	CYCGRG	2 cut(s)	1322, 1389
Aor13HI	TCCGGA	1 cut(s)	68
AoxI	GGCC	3 cut(s)	180, 337, 1419
ApeKI	GCWGC	5 cut(s)	356, 629, 666, 1351, 1759
ApoI	RAATTY	5 cut(s)	268, 561, 872, 1059, 1403
Asp700I	GAANNNNTTC	2 cut(s)	465, 1281
AspLEI	GCGC	1 cut(s)	59
AspS9I	GGNCC	5 cut(s)	296, 569, 683, 1420, 1733
AsuHPI	GGTGA	3 cut(s)	335, 509, 1814
AvaI	CYCGRG	2 cut(s)	1322, 1389
AvaII	GGWCC	4 cut(s)	296, 569, 683, 1733
BaeGI	GKGCMC	1 cut(s)	1521
BaeI	ACNNNNGTAYC	2 cut(s)	411, 444
BalI	TGGCCA	1 cut(s)	182
BanII	GRGCYC	3 cut(s)	165, 884, 1660
BauI	CACGAG	1 cut(s)	1723
BbsI	GAAGAC	1 cut(s)	239
Bbv12I	GWGCWC	2 cut(s)	165, 884
BbvI	GCAGC	5 cut(s)	343, 641, 678, 1363, 1746
BccI	CCATC	1 cut(s)	17
BceAI	ACGGC	1 cut(s)	1449
BciT130I	CCWGG	2 cut(s)	626, 1091
BciVI	GTATCC	1 cut(s)	412
BclI	TGATCA	2 cut(s)	397, 1428
BcoDI	GTCTC	2 cut(s)	1206, 1487
BfaI	CTAG	3 cut(s)	228, 635, 1056
BfoI	RGCGCY	1 cut(s)	60
BfuI	GTATCC	1 cut(s)	412
BglII	AGATCT	1 cut(s)	1717
BisI	GCNGC	5 cut(s)	357, 630, 667, 1352, 1760
BlsI	GCNGC	5 cut(s)	358, 631, 668, 1353, 1761
Bme1390I	CCNGG	2 cut(s)	626, 1091
Bme18I	GGWCC	4 cut(s)	296, 569, 683, 1733
BmeT110I	CYCGRG	2 cut(s)	1322, 1389
BmgT120I	GGNCC	5 cut(s)	296, 569, 683, 1420, 1733
BmiI	GGNNCC	3 cut(s)	1458, 1588, 1734
BmrFI	CCNGG	2 cut(s)	626, 1091
BmsI	GCATC	5 cut(s)	376, 400, 563, 1126, 1144
BpiI	GAAGAC	1 cut(s)	239
BpmI	CTGGAG	2 cut(s)	600, 1662
BpuEI	CTTGAG	1 cut(s)	1698
BsaHI	GRCGYC	1 cut(s)	1086
BsaI	GGTCTC	1 cut(s)	1487
BsaJI	CCNNGG	5 cut(s)	299, 823, 1321, 1390, 1660
BsaWI	WCCGGW	2 cut(s)	68, 571
BsaXI	ACNNNNNCTCC	4 cut(s)	234, 264, 370, 400
Bsc4I	CCNNNNNNNGG	6 cut(s)	86, 346, 578, 720, 920, 1226
Bse118I	RCCGGY	3 cut(s)	346, 1201, 1535
Bse1I	ACTGG	7 cut(s)	316, 914, 941, 1333, 1489, 1576, 1619
Bse3DI	GCAATG	3 cut(s)	357, 708, 813
BseAI	TCCGGA	1 cut(s)	68
BseBI	CCWGG	2 cut(s)	626, 1091
BseDI	CCNNGG	5 cut(s)	299, 823, 1321, 1390, 1660
BseGI	GGATG	7 cut(s)	270, 415, 554, 945, 1215, 1485, 1630
BseLI	CCNNNNNNNGG	6 cut(s)	86, 346, 578, 720, 920, 1226
BseMI	GCAATG	3 cut(s)	357, 708, 813
BseMII	CTCAG	3 cut(s)	279, 1680, 1754
BseNI	ACTGG	7 cut(s)	316, 914, 941, 1333, 1489, 1576, 1619
BseRI	GAGGAG	1 cut(s)	507
BseSI	GKGCMC	1 cut(s)	1521
BseXI	GCAGC	5 cut(s)	343, 641, 678, 1363, 1746
Bsh1236I	CGCG	1 cut(s)	391
Bsh1285I	CGRYCG	1 cut(s)	576
BshFI	GGCC	3 cut(s)	182, 339, 1421
BsiEI	CGRYCG	1 cut(s)	576
BsiHKAI	GWGCWC	2 cut(s)	165, 884
BsiHKCI	CYCGRG	2 cut(s)	1322, 1389
BsiSI	CCGG	5 cut(s)	69, 347, 572, 1202, 1536
BslFI	GGGAC	2 cut(s)	1339, 1397
BslI	CCNNNNNNNGG	6 cut(s)	86, 346, 578, 720, 920, 1226
BsmAI	GTCTC	2 cut(s)	1206, 1487
BsmFI	GGGAC	2 cut(s)	1339, 1397
BsmI	GAATGC	2 cut(s)	804, 1607
BsnI	GGCC	3 cut(s)	182, 339, 1421
Bso31I	GGTCTC	1 cut(s)	1487
BsoBI	CYCGRG	2 cut(s)	1322, 1389
Bsp1286I	GDGCHC	4 cut(s)	165, 884, 1521, 1660
Bsp13I	TCCGGA	1 cut(s)	68
Bsp1407I	TGTACA	1 cut(s)	1627
Bsp143I	GATC	8 cut(s)	71, 397, 499, 514, 1428, 1615, 1692, 1717
Bsp19I	CCATGG	1 cut(s)	299
BspACI	CCGC	4 cut(s)	389, 786, 1539, 1817
BspANI	GGCC	3 cut(s)	182, 339, 1421
BspCNI	CTCAG	3 cut(s)	280, 1681, 1755
BspEI	TCCGGA	1 cut(s)	68
BspFNI	CGCG	1 cut(s)	391
BspLI	GGNNCC	3 cut(s)	1458, 1588, 1734
BspPI	GGATC	3 cut(s)	79, 522, 1610
BspQI	GCTCTTC	1 cut(s)	1242
BspTNI	GGTCTC	1 cut(s)	1487
BsrBI	CCGCTC	1 cut(s)	1819
BsrDI	GCAATG	3 cut(s)	357, 708, 813
BsrFI	RCCGGY	3 cut(s)	346, 1201, 1535
BsrGI	TGTACA	1 cut(s)	1627
BsrI	ACTGG	7 cut(s)	316, 914, 941, 1333, 1489, 1576, 1619
BssAI	RCCGGY	3 cut(s)	346, 1201, 1535
BssECI	CCNNGG	5 cut(s)	299, 823, 1321, 1390, 1660
BssMI	GATC	8 cut(s)	71, 397, 499, 514, 1428, 1615, 1692, 1717
BssNAI	GTATAC	1 cut(s)	1119
BssNI	GRCGYC	1 cut(s)	1086
BssSI	CACGAG	1 cut(s)	1723
BssT1I	CCWWGG	2 cut(s)	299, 823
Bst1107I	GTATAC	1 cut(s)	1119
Bst2BI	CACGAG	1 cut(s)	1723
Bst2UI	CCWGG	2 cut(s)	626, 1091
Bst4CI	ACNGT	9 cut(s)	217, 433, 466, 979, 1126, 1147, 1516, 1555, 1648
Bst6I	CTCTTC	1 cut(s)	1242
BstACI	GRCGYC	1 cut(s)	1086
BstAPI	GCANNNNNTGC	2 cut(s)	356, 1501
BstAUI	TGTACA	1 cut(s)	1627
BstC8I	GCNNGC	2 cut(s)	1497, 1821
BstDEI	CTNAG	5 cut(s)	288, 605, 1689, 1740, 1763
BstDSI	CCRYGG	1 cut(s)	299
BstENI	CCTNNNNNAGG	1 cut(s)	1224
BstF5I	GGATG	7 cut(s)	270, 415, 554, 945, 1215, 1485, 1630
BstFNI	CGCG	1 cut(s)	391
BstH2I	RGCGCY	1 cut(s)	60
BstHHI	GCGC	1 cut(s)	59
BstKTI	GATC	8 cut(s)	74, 400, 502, 517, 1431, 1618, 1695, 1720
BstMAI	GTCTC	2 cut(s)	1206, 1487
BstMBI	GATC	8 cut(s)	71, 397, 499, 514, 1428, 1615, 1692, 1717
BstMCI	CGRYCG	1 cut(s)	576
BstNI	CCWGG	2 cut(s)	626, 1091
BstSCI	CCNGG	2 cut(s)	624, 1089
BstSLI	GKGCMC	1 cut(s)	1521
BstUI	CGCG	1 cut(s)	391
BstV1I	GCAGC	5 cut(s)	343, 641, 678, 1363, 1746
BstV2I	GAAGAC	1 cut(s)	239
BstX2I	RGATCY	1 cut(s)	1717
BstYI	RGATCY	1 cut(s)	1717
BstZ17I	GTATAC	1 cut(s)	1119
BsuI	GTATCC	1 cut(s)	412
BsuRI	GGCC	3 cut(s)	182, 339, 1421
BtgI	CCRYGG	1 cut(s)	299
BtsCI	GGATG	7 cut(s)	270, 415, 554, 945, 1215, 1485, 1630
BtsI	GCAGTG	3 cut(s)	12, 478, 726
BtsIMutI	CAGTG	6 cut(s)	12, 478, 726, 1551, 1569, 1626
Cac8I	GCNNGC	2 cut(s)	1497, 1821
CaiI	CAGNNNCTG	2 cut(s)	121, 1442
CfoI	GCGC	1 cut(s)	59
Cfr10I	RCCGGY	3 cut(s)	346, 1201, 1535
Cfr13I	GGNCC	5 cut(s)	296, 569, 683, 1420, 1733
CseI	GACGC	1 cut(s)	1518
Csp6I	GTAC	2 cut(s)	403, 1628
CviQI	GTAC	2 cut(s)	403, 1628
DdeI	CTNAG	5 cut(s)	288, 605, 1689, 1740, 1763
DpnI	GATC	8 cut(s)	73, 399, 501, 516, 1430, 1617, 1694, 1719
DpnII	GATC	8 cut(s)	71, 397, 499, 514, 1428, 1615, 1692, 1717
DrdI	GACNNNNNNGTC	1 cut(s)	1220
DseDI	GACNNNNNNGTC	1 cut(s)	1220
EaeI	YGGCCR	1 cut(s)	180
Eam1104I	CTCTTC	1 cut(s)	1242
EarI	CTCTTC	1 cut(s)	1242
Ecl136II	GAGCTC	2 cut(s)	163, 882
Eco130I	CCWWGG	2 cut(s)	299, 823
Eco147I	AGGCCT	1 cut(s)	339
Eco24I	GRGCYC	3 cut(s)	165, 884, 1660
Eco31I	GGTCTC	1 cut(s)	1487
Eco47I	GGWCC	4 cut(s)	296, 569, 683, 1733
Eco53kI	GAGCTC	2 cut(s)	163, 882
Eco57I	CTGAAG	1 cut(s)	996
Eco88I	CYCGRG	2 cut(s)	1322, 1389
EcoICRI	GAGCTC	2 cut(s)	163, 882
EcoNI	CCTNNNNNAGG	1 cut(s)	1224
EcoO109I	RGGNCCY	1 cut(s)	1733
EcoRI	GAATTC	2 cut(s)	872, 1059
EcoRII	CCWGG	2 cut(s)	624, 1089
EcoT14I	CCWWGG	2 cut(s)	299, 823
EcoT22I	ATGCAT	1 cut(s)	415
EcoT38I	GRGCYC	3 cut(s)	165, 884, 1660
ErhI	CCWWGG	2 cut(s)	299, 823
FaqI	GGGAC	2 cut(s)	1339, 1397
FauI	CCCGC	1 cut(s)	1532
FauNDI	CATATG	3 cut(s)	688, 1425, 1581
FbaI	TGATCA	2 cut(s)	397, 1428
FblI	GTMKAC	2 cut(s)	1118, 1548
Fnu4HI	GCNGC	5 cut(s)	357, 630, 667, 1352, 1760
FokI	GGATG	7 cut(s)	277, 422, 541, 952, 1202, 1472, 1637
FriOI	GRGCYC	3 cut(s)	165, 884, 1660
Fsp4HI	GCNGC	5 cut(s)	357, 630, 667, 1352, 1760
FspBI	CTAG	3 cut(s)	228, 635, 1056
GlaI	GCGC	1 cut(s)	58
GluI	GCNGC	5 cut(s)	357, 630, 667, 1352, 1760
GsuI	CTGGAG	2 cut(s)	600, 1662
HaeII	RGCGCY	1 cut(s)	60
HaeIII	GGCC	3 cut(s)	182, 339, 1421
HapII	CCGG	5 cut(s)	69, 347, 572, 1202, 1536
HgaI	GACGC	1 cut(s)	1518
HhaI	GCGC	1 cut(s)	59
Hin1I	GRCGYC	1 cut(s)	1086
Hin6I	GCGC	1 cut(s)	57
HinP1I	GCGC	1 cut(s)	57
HincII	GTYRAC	2 cut(s)	220, 1533
HindII	GTYRAC	2 cut(s)	220, 1533
HindIII	AAGCTT	1 cut(s)	22
HpaII	CCGG	5 cut(s)	69, 347, 572, 1202, 1536
HphI	GGTGA	3 cut(s)	335, 509, 1814
Hpy166II	GTNNAC	6 cut(s)	157, 220, 317, 1119, 1533, 1549
Hpy188I	TCNGA	9 cut(s)	76, 173, 654, 964, 1050, 1196, 1264, 1311, 1664
Hpy188III	TCNNGA	9 cut(s)	69, 240, 530, 681, 877, 973, 1056, 1324, 1715
Hpy8I	GTNNAC	6 cut(s)	157, 220, 317, 1119, 1533, 1549
HpyAV	CCTTC	5 cut(s)	626, 1249, 1556, 1600, 1723
HpyCH4III	ACNGT	9 cut(s)	217, 433, 466, 979, 1126, 1147, 1516, 1555, 1648
HpyCH4IV	ACGT	1 cut(s)	1086
HpyF3I	CTNAG	5 cut(s)	288, 605, 1689, 1740, 1763
HpySE526I	ACGT	1 cut(s)	1086
Hsp92I	GRCGYC	1 cut(s)	1086
HspAI	GCGC	1 cut(s)	57
Kpn2I	TCCGGA	1 cut(s)	68
Ksp22I	TGATCA	2 cut(s)	397, 1428
Kzo9I	GATC	8 cut(s)	71, 397, 499, 514, 1428, 1615, 1692, 1717
LguI	GCTCTTC	1 cut(s)	1242
LmnI	GCTCC	3 cut(s)	168, 1439, 1586
Lsp1109I	GCAGC	5 cut(s)	343, 641, 678, 1363, 1746
LweI	GCATC	5 cut(s)	376, 400, 563, 1126, 1144
MaeI	CTAG	3 cut(s)	228, 635, 1056
MaeII	ACGT	1 cut(s)	1086
MaeIII	GTNAC	3 cut(s)	427, 1105, 1648
MalI	GATC	8 cut(s)	73, 399, 501, 516, 1430, 1617, 1694, 1719
MbiI	CCGCTC	1 cut(s)	1819
MboI	GATC	8 cut(s)	71, 397, 499, 514, 1428, 1615, 1692, 1717
MboII	GAAGA	6 cut(s)	244, 707, 1002, 1010, 1037, 1259
MfeI	CAATTG	1 cut(s)	1635
MflI	RGATCY	1 cut(s)	1717
MhlI	GDGCHC	4 cut(s)	165, 884, 1521, 1660
MlsI	TGGCCA	1 cut(s)	182
MluNI	TGGCCA	1 cut(s)	182
MlyI	GAGTC	3 cut(s)	118, 252, 591
MmeI	TCCRAC	1 cut(s)	1174
Mox20I	TGGCCA	1 cut(s)	182
Mph1103I	ATGCAT	1 cut(s)	415
MroI	TCCGGA	1 cut(s)	68
MroXI	GAANNNNTTC	2 cut(s)	465, 1281
MscI	TGGCCA	1 cut(s)	182
MseI	TTAA	4 cut(s)	93, 441, 660, 1557
Msp20I	TGGCCA	1 cut(s)	182
MspA1I	CMGCKG	1 cut(s)	1363
MspI	CCGG	5 cut(s)	69, 347, 572, 1202, 1536
MspR9I	CCNGG	2 cut(s)	626, 1091
MunI	CAATTG	1 cut(s)	1635
Mva1269I	GAATGC	2 cut(s)	804, 1607
MvaI	CCWGG	2 cut(s)	626, 1091
MvnI	CGCG	1 cut(s)	391
NcoI	CCATGG	1 cut(s)	299
NdeI	CATATG	3 cut(s)	688, 1425, 1581
NdeII	GATC	8 cut(s)	71, 397, 499, 514, 1428, 1615, 1692, 1717
NlaIV	GGNNCC	3 cut(s)	1458, 1588, 1734
NmuCI	GTSAC	2 cut(s)	427, 1648
NsiI	ATGCAT	1 cut(s)	415
PceI	AGGCCT	1 cut(s)	339
PciSI	GCTCTTC	1 cut(s)	1242
PcsI	WCGNNNNNNNCGW	2 cut(s)	582, 1782
PctI	GAATGC	2 cut(s)	804, 1607
PdmI	GAANNNNTTC	2 cut(s)	465, 1281
PfeI	GAWTC	7 cut(s)	174, 609, 951, 959, 1099, 1467, 1566
PflFI	GACNNNGTC	1 cut(s)	968
PflMI	CCANNNNNTGG	1 cut(s)	720
PfoI	TCCNGGA	1 cut(s)	1089
PkrI	GCNGC	5 cut(s)	358, 631, 668, 1353, 1761
PleI	GAGTC	3 cut(s)	118, 251, 590
PpsI	GAGTC	3 cut(s)	118, 251, 590
PpuMI	RGGWCCY	1 cut(s)	1733
Psp124BI	GAGCTC	2 cut(s)	165, 884
Psp5II	RGGWCCY	1 cut(s)	1733
Psp6I	CCWGG	2 cut(s)	624, 1089
PspGI	CCWGG	2 cut(s)	624, 1089
PspN4I	GGNNCC	3 cut(s)	1458, 1588, 1734
PspPI	GGNCC	5 cut(s)	296, 569, 683, 1420, 1733
PspPPI	RGGWCCY	1 cut(s)	1733
PstNI	CAGNNNCTG	2 cut(s)	121, 1442
PsuI	RGATCY	1 cut(s)	1717
PsyI	GACNNNGTC	1 cut(s)	968
PvuII	CAGCTG	1 cut(s)	1363
RsaI	GTAC	2 cut(s)	404, 1629
RsaNI	GTAC	2 cut(s)	403, 1628
SacI	GAGCTC	2 cut(s)	165, 884
SapI	GCTCTTC	1 cut(s)	1242
SaqAI	TTAA	4 cut(s)	93, 441, 660, 1557
SatI	GCNGC	5 cut(s)	357, 630, 667, 1352, 1760
Sau3AI	GATC	8 cut(s)	71, 397, 499, 514, 1428, 1615, 1692, 1717
Sau96I	GGNCC	5 cut(s)	296, 569, 683, 1420, 1733
SchI	GAGTC	3 cut(s)	118, 252, 591
ScrFI	CCNGG	2 cut(s)	626, 1091
SduI	GDGCHC	4 cut(s)	165, 884, 1521, 1660
SfaNI	GCATC	5 cut(s)	376, 400, 563, 1126, 1144
SinI	GGWCC	4 cut(s)	296, 569, 683, 1733
SmlI	CTYRAG	1 cut(s)	1713
SmoI	CTYRAG	1 cut(s)	1713
SseBI	AGGCCT	1 cut(s)	339
SsiI	CCGC	4 cut(s)	389, 786, 1539, 1817
SspI	AATATT	1 cut(s)	674
SspMI	CTAG	3 cut(s)	228, 635, 1056
SstI	GAGCTC	2 cut(s)	165, 884
StuI	AGGCCT	1 cut(s)	339
StyD4I	CCNGG	2 cut(s)	624, 1089
StyI	CCWWGG	2 cut(s)	299, 823
TaaI	ACNGT	9 cut(s)	217, 433, 466, 979, 1126, 1147, 1516, 1555, 1648
TaiI	ACGT	1 cut(s)	1089
TaqI	TCGA	5 cut(s)	370, 469, 531, 585, 1470
TatI	WGTACW	2 cut(s)	402, 1627
TfiI	GAWTC	7 cut(s)	174, 609, 951, 959, 1099, 1467, 1566
Tru1I	TTAA	4 cut(s)	93, 441, 660, 1557
Tru9I	TTAA	4 cut(s)	93, 441, 660, 1557
TscAI	CASTG	6 cut(s)	19, 485, 733, 1558, 1576, 1626
TseFI	GTSAC	2 cut(s)	427, 1648
TseI	GCWGC	5 cut(s)	356, 629, 666, 1351, 1759
Tsp45I	GTSAC	2 cut(s)	427, 1648
TspDTI	ATGAA	8 cut(s)	281, 697, 883, 885, 956, 1222, 1438, 1568
TspGWI	ACGGA	1 cut(s)	155
TspRI	CASTG	6 cut(s)	19, 485, 733, 1558, 1576, 1626
Tth111I	GACNNNGTC	1 cut(s)	968
Van91I	CCANNNNNTGG	1 cut(s)	720
VpaK11BI	GGWCC	4 cut(s)	296, 569, 683, 1733
XagI	CCTNNNNNAGG	1 cut(s)	1224
XapI	RAATTY	5 cut(s)	268, 561, 872, 1059, 1403
XbaI	TCTAGA	1 cut(s)	1055
XcmI	CCANNNNNNNNNTGG	1 cut(s)	50
XmiI	GTMKAC	2 cut(s)	1118, 1548
XmnI	GAANNNNTTC	2 cut(s)	465, 1281
XspI	CTAG	3 cut(s)	228, 635, 1056
ZraI	GACGTC	1 cut(s)	1087
Zsp2I	ATGCAT	1 cut(s)	415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.