Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003916

Physical Location & Seq

Genomic Coordinates Reverse (-)

27698599 .. 27701463

Gene Structure 2865 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1198700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGTCCTATCCCTTTCCCCTTATGTCCTATCCCTTTCCCCACTATAAATACGACTACCAAGTTGCCGACACCCCAATCCTACAAAGGAAAAGGAGAGGATCCACCGCCTTCCCAAAGCACCACCACAAAAAGAAAAAAGCAACCATGGCCACCACCAAGCTCCTTCTCTCCCTTCTCCTCCTCGTCCAGCTCGCCGCCACAGCCTACTCCATCGGCGTAAACTACGGTACTCTCGCCGACAACCTCCCGCCACCGGCCCAAGTCGCCGACTTCATCAAAACCCAAACCAACATCGACGCCGTCAAACTCTTCGACGCCAACCCCGACATCATCAAGGCATTCGCCAACACCAACATCTCCTTGACGATTACCATCCCCAACGGAGACATCCAGAGCCTCATAAATCTACGCACCGCTCGCCATTGGATCATTGATCACGTCAAGCCGTTTTACCCGGCAACCAAAATCAAATACATTGCCATGGGCAGCGAGGTCCTCCACTGGGGGGACGACGCTCTCAAGGCGAGCCTCGTCCCTGCCATGAGGACCTTCCACAACGCTTTGATCCGTGAGGGAATCGAGGACGTCAAGGTCTCGACCCCTCACTCGCTCGGAATGATGTTGTCTTCCGACCCGCCCAGCACAGGCCGGTTCAGACCGGAGGTGCATCCAATTCTGGCCCAAATCCTTCTGTTCTGTCGCCAGACCAAGTCGCCTTTCATGGTTAACCCGTACCCGTTTTTCGGGTGGTCACCCGAAAAGGAGAACTACGCTCTCTTCAGACCCAACAGTGGTGTGCGCGATAAATTCACCGGCAAGTTTTACACTAACATGTTCGATGGGTTGATGGACGCGGTTTACTCAGCTGCCAAGGCAATCGGGTTCGGTGACGTGAACTTGATTGCGGGCGAGACCGGTTGGCCTTCGGCTTGCGAGCTCCCAGTCTGCTCGGTTCAGAACGCTGCGGACTACAACGGTAACTTGATCAAGCATATTGAGTCCGGTAAGGGTACGCCATTGATGCCGAACCGTACGATCGAGACTTACATCTTTGCTTTGTTTAATGAGAACCAGAAACCCGGTTCGGCCGCTGAGAAGAACTTTGGGCTGTTCAAACCGGACATGACTCCGGTTTACAATGCTGGGGTTATGCGCAATCAACAGGATGGTGCAACTCCAGGAAAGCCTGCCGGACCAGCAAAACCATCAACACCAGCGACGCCGACAACACCGGTAGCAGGAGGCAAGAAGTGGTGCGTGGCAAAATCAGCAGCCAGCAACCAAGCATTGCAATCAAACATTGACTACGTCTGTAGCACCGGAGTTGATTGCAAGTCCATCCAGCCAGGCGGCGCTTGCTTTGATAACGACGTTAGAGCCCGCGCGTCTTACCTTATGAATGCTTACTACCAGGCTAACGGCCGTCACGACTTCAACTGTGATTTCTCTGGGTCCGGACAAATCACCACCACCGACCCAAGCCACGGCTCTTGTAAATACAATTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001871 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010208 GO:0010584 GO:0010927 GO:0016020 GO:0016043 GO:0016787 GO:0016798 GO:0022414 GO:0022607 GO:0030198 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0044085 GO:0044425 GO:0044459 GO:0044464 GO:0045229 GO:0046658 GO:0048229 GO:0048316 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0061458 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

503

Amino Acids

55.02

Weight (kDa)

9.04

Isoelectric Point (pI)

40.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	71 - 380	1e-81	Glycosyl hydrolases family 17
X8	PF07983	417 - 486	1.4e-19	X8 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003916)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55430
fragaria_vesca	FvH4_7g22480 FvH4_7g22494
malus_domestica	MD01G1134200.v1.1 MD07G1198400.v1.1 MD07G1198600.v1.1 MD07G1198700.v1.1
prunus_persica	Prupe.2G235400_v2.0.a1
pyrus_communis	pycom01g15500 pycom07g18550 pycom07g18560 pycom07g18600
rosa_chinensis	RchiOBHm_Chr1g0367091 RchiOBHm_Chr1g0367121
rosa_laevigata	RLG00000027296 RLG00000027300
rosa_multiflora	Rmu_co8239959.1_g000001 Rmu_co8287615.1_g000001 Rmu_sc0007424.1_g000003 Rmu_sc0007844.1_g000002
rosa_roxburghii	Rroxscaffold_4G00289610 Rroxscaffold_4G00289630
rosa_rugosa	Rorug01G0334300 Rorug01G0334700 Rorug01G0334800
rosa_samantha	Rh1AG341300 Rh1AG341500 Rh1BG302000 Rh1CG318000 Rh1CG318400 Rh1DG334200 Rh1DG334500
rosa_wichuraiana	Rw1G030320 Rw1G030350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	590
AatII	GACGTC	1 cut(s)	588
Acc16I	TGCGCA	1 cut(s)	1154
AccBSI	CCGCTC	1 cut(s)	416
AccII	CGCG	4 cut(s)	801, 854, 1383, 1385
AccIII	TCCGGA	1 cut(s)	1454
AclWI	GGATC	4 cut(s)	93, 106, 434, 559
AcoI	YGGCCR	3 cut(s)	147, 1086, 1420
AcsI	RAATTY	1 cut(s)	806
AcuI	CTGAAG	1 cut(s)	763
AcyI	GRCGYC	4 cut(s)	297, 315, 585, 1220
AfaI	GTAC	4 cut(s)	229, 734, 1012, 1033
AfiI	CCNNNNNNNGG	8 cut(s)	253, 502, 505, 644, 743, 791, 1238, 1484
AflIII	ACRYGT	1 cut(s)	831
AgeI	ACCGGT	2 cut(s)	914, 1231
AgsI	TTSAA	2 cut(s)	1114, 1435
AjiI	CACGTC	2 cut(s)	439, 892
AjnI	CCWGG	3 cut(s)	1177, 1345, 1410
AjuI	GAANNNNNNNTTGG	2 cut(s)	701, 733
AluBI	AGCT	4 cut(s)	160, 190, 866, 937
AluI	AGCT	4 cut(s)	160, 190, 866, 937
Alw21I	GWGCWC	1 cut(s)	939
Alw26I	GTCTC	4 cut(s)	378, 598, 905, 1034
AlwI	GGATC	4 cut(s)	93, 106, 434, 559
Aor13HI	TCCGGA	1 cut(s)	1454
AoxI	GGCC	7 cut(s)	147, 255, 646, 678, 920, 1086, 1420
ApeKI	GCWGC	4 cut(s)	486, 866, 962, 1271
ApoI	RAATTY	1 cut(s)	806
AsiGI	ACCGGT	2 cut(s)	914, 1231
AspLEI	GCGC	4 cut(s)	801, 1155, 1355, 1385
AspS9I	GGNCC	6 cut(s)	256, 493, 546, 679, 1193, 1452
AsuC2I	CCSGG	2 cut(s)	455, 1080
AsuHPI	GGTGA	4 cut(s)	744, 802, 899, 1456
AvaII	GGWCC	4 cut(s)	493, 546, 1193, 1452
BalI	TGGCCA	1 cut(s)	149
BamHI	GGATCC	1 cut(s)	98
BanII	GRGCYC	2 cut(s)	939, 1381
BbsI	GAAGAC	1 cut(s)	618
Bbv12I	GWGCWC	1 cut(s)	939
BbvI	GCAGC	4 cut(s)	498, 853, 949, 1283
BccI	CCATC	7 cut(s)	218, 380, 833, 841, 1160, 1213, 1346
BceAI	ACGGC	5 cut(s)	284, 430, 1407, 1435, 1501
BciT130I	CCWGG	3 cut(s)	1179, 1347, 1412
BclI	TGATCA	2 cut(s)	433, 984
BcnI	CCSGG	2 cut(s)	455, 1080
BcoDI	GTCTC	4 cut(s)	378, 598, 905, 1034
BfmI	CTRYAG	1 cut(s)	1312
BfoI	RGCGCY	1 cut(s)	1356
BisI	GCNGC	7 cut(s)	195, 487, 867, 963, 1089, 1272, 1351
BlsI	GCNGC	7 cut(s)	196, 488, 868, 964, 1090, 1273, 1352
Bme1390I	CCNGG	5 cut(s)	455, 1080, 1179, 1347, 1412
Bme18I	GGWCC	4 cut(s)	493, 546, 1193, 1452
BmgBI	CACGTC	2 cut(s)	439, 892
BmgT120I	GGNCC	6 cut(s)	256, 493, 546, 679, 1193, 1452
BmiI	GGNNCC	2 cut(s)	100, 1453
BmrFI	CCNGG	5 cut(s)	455, 1080, 1179, 1347, 1412
BmrI	ACTGGG	2 cut(s)	511, 935
BmsI	GCATC	2 cut(s)	676, 1011
BmuI	ACTGGG	2 cut(s)	511, 935
BpiI	GAAGAC	1 cut(s)	618
BpmI	CTGGAG	1 cut(s)	1161
BpuEI	CTTGAG	1 cut(s)	503
BpuMI	CCSGG	2 cut(s)	455, 1080
BsaBI	GATNNNNATC	1 cut(s)	371
BsaHI	GRCGYC	4 cut(s)	297, 315, 585, 1220
BsaI	GGTCTC	2 cut(s)	598, 905
BsaJI	CCNNGG	4 cut(s)	144, 480, 870, 1483
BsaWI	WCCGGW	8 cut(s)	658, 914, 1001, 1117, 1129, 1231, 1319, 1454
Bsc4I	CCNNNNNNNGG	8 cut(s)	253, 502, 505, 644, 743, 791, 1238, 1484
Bse118I	RCCGGY	5 cut(s)	253, 648, 812, 914, 1231
Bse1I	ACTGG	2 cut(s)	506, 941
Bse3DI	GCAATG	2 cut(s)	474, 1286
Bse8I	GATNNNNATC	1 cut(s)	371
BseAI	TCCGGA	1 cut(s)	1454
BseBI	CCWGG	3 cut(s)	1179, 1347, 1412
BseDI	CCNNGG	4 cut(s)	144, 480, 870, 1483
BseGI	GGATG	5 cut(s)	372, 387, 667, 1171, 1338
BseJI	GATNNNNATC	1 cut(s)	371
BseLI	CCNNNNNNNGG	8 cut(s)	253, 502, 505, 644, 743, 791, 1238, 1484
BseMI	GCAATG	2 cut(s)	474, 1286
BseMII	CTCAG	2 cut(s)	876, 1083
BseNI	ACTGG	2 cut(s)	506, 941
BseRI	GAGGAG	2 cut(s)	167, 170
BseX3I	CGGCCG	2 cut(s)	1086, 1420
BseXI	GCAGC	4 cut(s)	498, 853, 949, 1283
BseYI	CCCAGC	2 cut(s)	638, 1142
Bsh1236I	CGCG	4 cut(s)	801, 854, 1383, 1385
Bsh1285I	CGRYCG	3 cut(s)	1038, 1089, 1423
BshFI	GGCC	7 cut(s)	149, 257, 648, 680, 922, 1088, 1422
BshTI	ACCGGT	2 cut(s)	914, 1231
BsiEI	CGRYCG	3 cut(s)	1038, 1089, 1423
BsiHKAI	GWGCWC	1 cut(s)	939
BsiWI	CGTACG	1 cut(s)	1031
BslFI	GGGAC	2 cut(s)	518, 521
BslI	CCNNNNNNNGG	8 cut(s)	253, 502, 505, 644, 743, 791, 1238, 1484
BsmAI	GTCTC	4 cut(s)	378, 598, 905, 1034
BsmFI	GGGAC	2 cut(s)	518, 521
BsmI	GAATGC	2 cut(s)	338, 1405
BsnI	GGCC	7 cut(s)	149, 257, 648, 680, 922, 1088, 1422
Bso31I	GGTCTC	2 cut(s)	598, 905
Bsp1286I	GDGCHC	2 cut(s)	939, 1381
Bsp13I	TCCGGA	1 cut(s)	1454
Bsp143I	GATC	6 cut(s)	98, 426, 433, 564, 984, 1035
Bsp19I	CCATGG	2 cut(s)	144, 480
BspANI	GGCC	7 cut(s)	149, 257, 648, 680, 922, 1088, 1422
BspCNI	CTCAG	2 cut(s)	875, 1084
BspEI	TCCGGA	1 cut(s)	1454
BspFNI	CGCG	4 cut(s)	801, 854, 1383, 1385
BspLI	GGNNCC	2 cut(s)	100, 1453
BspPI	GGATC	4 cut(s)	93, 106, 434, 559
BspTNI	GGTCTC	2 cut(s)	598, 905
BsrBI	CCGCTC	1 cut(s)	416
BsrDI	GCAATG	2 cut(s)	474, 1286
BsrFI	RCCGGY	5 cut(s)	253, 648, 812, 914, 1231
BsrI	ACTGG	2 cut(s)	506, 941
BssAI	RCCGGY	5 cut(s)	253, 648, 812, 914, 1231
BssECI	CCNNGG	4 cut(s)	144, 480, 870, 1483
BssMI	GATC	6 cut(s)	98, 426, 433, 564, 984, 1035
BssNI	GRCGYC	4 cut(s)	297, 315, 585, 1220
BssT1I	CCWWGG	3 cut(s)	144, 480, 870
Bst2UI	CCWGG	3 cut(s)	1179, 1347, 1412
Bst4CI	ACNGT	5 cut(s)	227, 791, 977, 1031, 1439
Bst6I	CTCTTC	2 cut(s)	314, 782
BstACI	GRCGYC	4 cut(s)	297, 315, 585, 1220
BstDEI	CTNAG	2 cut(s)	862, 1092
BstDSI	CCRYGG	3 cut(s)	144, 480, 1483
BstEII	GGTNACC	1 cut(s)	750
BstF5I	GGATG	5 cut(s)	372, 387, 667, 1171, 1338
BstFNI	CGCG	4 cut(s)	801, 854, 1383, 1385
BstH2I	RGCGCY	1 cut(s)	1356
BstHHI	GCGC	4 cut(s)	801, 1155, 1355, 1385
BstKTI	GATC	6 cut(s)	101, 429, 436, 567, 987, 1038
BstMAI	GTCTC	4 cut(s)	378, 598, 905, 1034
BstMBI	GATC	6 cut(s)	98, 426, 433, 564, 984, 1035
BstMCI	CGRYCG	3 cut(s)	1038, 1089, 1423
BstNI	CCWGG	3 cut(s)	1179, 1347, 1412
BstNSI	RCATGY	1 cut(s)	835
BstPI	GGTNACC	1 cut(s)	750
BstSCI	CCNGG	5 cut(s)	453, 1078, 1177, 1345, 1410
BstSFI	CTRYAG	1 cut(s)	1312
BstUI	CGCG	4 cut(s)	801, 854, 1383, 1385
BstV1I	GCAGC	4 cut(s)	498, 853, 949, 1283
BstV2I	GAAGAC	1 cut(s)	618
BstX2I	RGATCY	1 cut(s)	98
BstYI	RGATCY	1 cut(s)	98
BstZI	CGGCCG	2 cut(s)	1086, 1420
BsuRI	GGCC	7 cut(s)	149, 257, 648, 680, 922, 1088, 1422
BtgI	CCRYGG	3 cut(s)	144, 480, 1483
BtrI	CACGTC	2 cut(s)	439, 892
BtsCI	GGATG	5 cut(s)	372, 387, 667, 1171, 1338
BtsIMutI	CAGTG	2 cut(s)	499, 796
CfoI	GCGC	4 cut(s)	801, 1155, 1355, 1385
Cfr10I	RCCGGY	5 cut(s)	253, 648, 812, 914, 1231
Cfr13I	GGNCC	6 cut(s)	256, 493, 546, 679, 1193, 1452
CseI	GACGC	6 cut(s)	305, 323, 521, 860, 1228, 1374
Csp6I	GTAC	4 cut(s)	228, 733, 1011, 1032
CspAI	ACCGGT	2 cut(s)	914, 1231
CspCI	CAANNNNNGTGG	2 cut(s)	1472, 1507
CviAII	CATG	6 cut(s)	145, 481, 541, 721, 832, 1123
CviQI	GTAC	4 cut(s)	228, 733, 1011, 1032
DdeI	CTNAG	2 cut(s)	862, 1092
DpnI	GATC	6 cut(s)	100, 428, 435, 566, 986, 1037
DpnII	GATC	6 cut(s)	98, 426, 433, 564, 984, 1035
DrdI	GACNNNNNNGTC	1 cut(s)	590
DseDI	GACNNNNNNGTC	1 cut(s)	590
EaeI	YGGCCR	3 cut(s)	147, 1086, 1420
EagI	CGGCCG	2 cut(s)	1086, 1420
Eam1104I	CTCTTC	2 cut(s)	314, 782
EarI	CTCTTC	2 cut(s)	314, 782
Ecl136II	GAGCTC	1 cut(s)	937
EclXI	CGGCCG	2 cut(s)	1086, 1420
Eco130I	CCWWGG	3 cut(s)	144, 480, 870
Eco24I	GRGCYC	2 cut(s)	939, 1381
Eco31I	GGTCTC	2 cut(s)	598, 905
Eco47I	GGWCC	4 cut(s)	493, 546, 1193, 1452
Eco52I	CGGCCG	2 cut(s)	1086, 1420
Eco53kI	GAGCTC	1 cut(s)	937
Eco57I	CTGAAG	1 cut(s)	763
Eco91I	GGTNACC	1 cut(s)	750
EcoICRI	GAGCTC	1 cut(s)	937
EcoO109I	RGGNCCY	2 cut(s)	493, 546
EcoO65I	GGTNACC	1 cut(s)	750
EcoRII	CCWGG	3 cut(s)	1177, 1345, 1410
EcoT14I	CCWWGG	3 cut(s)	144, 480, 870
EcoT38I	GRGCYC	2 cut(s)	939, 1381
ErhI	CCWWGG	3 cut(s)	144, 480, 870
FaeI	CATG	6 cut(s)	148, 484, 544, 724, 835, 1126
FaqI	GGGAC	2 cut(s)	518, 521
FatI	CATG	6 cut(s)	144, 480, 540, 720, 831, 1122
FauI	CCCGC	4 cut(s)	255, 642, 898, 1388
FbaI	TGATCA	2 cut(s)	433, 984
Fnu4HI	GCNGC	7 cut(s)	195, 487, 867, 963, 1089, 1272, 1351
FokI	GGATG	5 cut(s)	359, 374, 654, 1178, 1325
FriOI	GRGCYC	2 cut(s)	939, 1381
Fsp4HI	GCNGC	7 cut(s)	195, 487, 867, 963, 1089, 1272, 1351
FspI	TGCGCA	1 cut(s)	1154
GlaI	GCGC	4 cut(s)	800, 1154, 1354, 1384
GluI	GCNGC	7 cut(s)	195, 487, 867, 963, 1089, 1272, 1351
GsaI	CCCAGC	2 cut(s)	642, 1146
GsuI	CTGGAG	1 cut(s)	1161
HaeII	RGCGCY	1 cut(s)	1356
HaeIII	GGCC	7 cut(s)	149, 257, 648, 680, 922, 1088, 1422
HgaI	GACGC	6 cut(s)	305, 323, 521, 860, 1228, 1374
HhaI	GCGC	4 cut(s)	801, 1155, 1355, 1385
Hin1I	GRCGYC	4 cut(s)	297, 315, 585, 1220
Hin1II	CATG	6 cut(s)	148, 484, 544, 724, 835, 1126
Hin6I	GCGC	4 cut(s)	799, 1153, 1353, 1383
HinP1I	GCGC	4 cut(s)	799, 1153, 1353, 1383
HincII	GTYRAC	1 cut(s)	727
HindII	GTYRAC	1 cut(s)	727
HinfI	GANTC	3 cut(s)	576, 998, 1126
HpaI	GTTAAC	1 cut(s)	727
HphI	GGTGA	4 cut(s)	744, 802, 899, 1456
Hpy166II	GTNNAC	5 cut(s)	220, 727, 859, 895, 1135
Hpy188I	TCNGA	5 cut(s)	614, 631, 656, 782, 957
Hpy188III	TCNNGA	6 cut(s)	391, 595, 1039, 1427, 1455, 1506
Hpy8I	GTNNAC	5 cut(s)	220, 727, 859, 895, 1135
Hpy99I	CGWCG	5 cut(s)	299, 317, 515, 1222, 1373
HpyAV	CCTTC	6 cut(s)	118, 173, 182, 559, 698, 933
HpyCH4III	ACNGT	5 cut(s)	227, 791, 977, 1031, 1439
HpyCH4IV	ACGT	5 cut(s)	438, 585, 891, 1308, 1371
HpyCH4V	TGCA	4 cut(s)	667, 1172, 1291, 1332
HpyF3I	CTNAG	2 cut(s)	862, 1092
HpySE526I	ACGT	5 cut(s)	438, 585, 891, 1308, 1371
Hsp92I	GRCGYC	4 cut(s)	297, 315, 585, 1220
Hsp92II	CATG	6 cut(s)	148, 484, 544, 724, 835, 1126
HspAI	GCGC	4 cut(s)	799, 1153, 1353, 1383
Kpn2I	TCCGGA	1 cut(s)	1454
Ksp22I	TGATCA	2 cut(s)	433, 984
KspAI	GTTAAC	1 cut(s)	727
Kzo9I	GATC	6 cut(s)	98, 426, 433, 564, 984, 1035
LmnI	GCTCC	2 cut(s)	165, 942
Lsp1109I	GCAGC	4 cut(s)	498, 853, 949, 1283
LweI	GCATC	2 cut(s)	676, 1011
MaeII	ACGT	5 cut(s)	438, 585, 891, 1308, 1371
MaeIII	GTNAC	4 cut(s)	750, 887, 977, 1424
MalI	GATC	6 cut(s)	100, 428, 435, 566, 986, 1037
MbiI	CCGCTC	1 cut(s)	416
MboI	GATC	6 cut(s)	98, 426, 433, 564, 984, 1035
MboII	GAAGA	4 cut(s)	301, 618, 769, 1108
MflI	RGATCY	1 cut(s)	98
MhlI	GDGCHC	2 cut(s)	939, 1381
MlsI	TGGCCA	1 cut(s)	149
MluCI	AATT	3 cut(s)	672, 806, 1501
MluNI	TGGCCA	1 cut(s)	149
MlyI	GAGTC	2 cut(s)	1007, 1120
MmeI	TCCRAC	1 cut(s)	654
Mox20I	TGGCCA	1 cut(s)	149
MroI	TCCGGA	1 cut(s)	1454
MscI	TGGCCA	1 cut(s)	149
MseI	TTAA	2 cut(s)	726, 1062
MslI	CAYNNNNRTG	2 cut(s)	479, 830
Msp20I	TGGCCA	1 cut(s)	149
MspA1I	CMGCKG	2 cut(s)	866, 1091
MspR9I	CCNGG	5 cut(s)	455, 1080, 1179, 1347, 1412
Mva1269I	GAATGC	2 cut(s)	338, 1405
MvaI	CCWGG	3 cut(s)	1179, 1347, 1412
MvnI	CGCG	4 cut(s)	801, 854, 1383, 1385
NciI	CCSGG	2 cut(s)	455, 1080
NcoI	CCATGG	2 cut(s)	144, 480
NdeII	GATC	6 cut(s)	98, 426, 433, 564, 984, 1035
NlaIII	CATG	6 cut(s)	148, 484, 544, 724, 835, 1126
NlaIV	GGNNCC	2 cut(s)	100, 1453
NmuCI	GTSAC	3 cut(s)	750, 887, 1424
NsbI	TGCGCA	1 cut(s)	1154
NspI	RCATGY	1 cut(s)	835
PciI	ACATGT	1 cut(s)	831
PcsI	WCGNNNNNNNCGW	2 cut(s)	321, 1425
PctI	GAATGC	2 cut(s)	338, 1405
PfeI	GAWTC	1 cut(s)	576
Pfl23II	CGTACG	1 cut(s)	1031
PflFI	GACNNNGTC	3 cut(s)	299, 709, 1307
PfoI	TCCNGGA	1 cut(s)	1177
PinAI	ACCGGT	2 cut(s)	914, 1231
PkrI	GCNGC	7 cut(s)	196, 488, 868, 964, 1090, 1273, 1352
Ple19I	CGATCG	1 cut(s)	1038
PleI	GAGTC	2 cut(s)	1006, 1120
PpsI	GAGTC	2 cut(s)	1006, 1120
PpuMI	RGGWCCY	2 cut(s)	493, 546
PscI	ACATGT	1 cut(s)	831
Psp124BI	GAGCTC	1 cut(s)	939
Psp5II	RGGWCCY	2 cut(s)	493, 546
Psp6I	CCWGG	3 cut(s)	1177, 1345, 1410
PspEI	GGTNACC	1 cut(s)	750
PspFI	CCCAGC	2 cut(s)	638, 1142
PspGI	CCWGG	3 cut(s)	1177, 1345, 1410
PspLI	CGTACG	1 cut(s)	1031
PspN4I	GGNNCC	2 cut(s)	100, 1453
PspPI	GGNCC	6 cut(s)	256, 493, 546, 679, 1193, 1452
PspPPI	RGGWCCY	2 cut(s)	493, 546
PsuI	RGATCY	1 cut(s)	98
PsyI	GACNNNGTC	3 cut(s)	299, 709, 1307
PvuI	CGATCG	1 cut(s)	1038
PvuII	CAGCTG	1 cut(s)	866
RsaI	GTAC	4 cut(s)	229, 734, 1012, 1033
RsaNI	GTAC	4 cut(s)	228, 733, 1011, 1032
RseI	CAYNNNNRTG	2 cut(s)	479, 830
SacI	GAGCTC	1 cut(s)	939
SaqAI	TTAA	2 cut(s)	726, 1062
SatI	GCNGC	7 cut(s)	195, 487, 867, 963, 1089, 1272, 1351
Sau3AI	GATC	6 cut(s)	98, 426, 433, 564, 984, 1035
Sau96I	GGNCC	6 cut(s)	256, 493, 546, 679, 1193, 1452
SchI	GAGTC	2 cut(s)	1007, 1120
ScrFI	CCNGG	5 cut(s)	455, 1080, 1179, 1347, 1412
SduI	GDGCHC	2 cut(s)	939, 1381
SfaNI	GCATC	2 cut(s)	676, 1011
SfcI	CTRYAG	1 cut(s)	1312
SinI	GGWCC	4 cut(s)	493, 546, 1193, 1452
SmiMI	CAYNNNNRTG	2 cut(s)	479, 830
SmlI	CTYRAG	1 cut(s)	518
SmoI	CTYRAG	1 cut(s)	518
Sse9I	AATT	3 cut(s)	672, 806, 1501
SstI	GAGCTC	1 cut(s)	939
StyD4I	CCNGG	5 cut(s)	453, 1078, 1177, 1345, 1410
StyI	CCWWGG	3 cut(s)	144, 480, 870
TaaI	ACNGT	5 cut(s)	227, 791, 977, 1031, 1439
TaiI	ACGT	5 cut(s)	441, 588, 894, 1311, 1374
TaqI	TCGA	6 cut(s)	294, 312, 579, 596, 837, 1038
TasI	AATT	3 cut(s)	672, 806, 1501
TauI	GCSGC	3 cut(s)	197, 1091, 1353
TfiI	GAWTC	1 cut(s)	576
Tru1I	TTAA	2 cut(s)	726, 1062
Tru9I	TTAA	2 cut(s)	726, 1062
TscAI	CASTG	2 cut(s)	506, 796
TseFI	GTSAC	3 cut(s)	750, 887, 1424
TseI	GCWGC	4 cut(s)	486, 866, 962, 1271
Tsp45I	GTSAC	3 cut(s)	750, 887, 1424
TspDTI	ATGAA	3 cut(s)	262, 709, 1412
TspGWI	ACGGA	2 cut(s)	396, 557
TspRI	CASTG	2 cut(s)	506, 796
Tth111I	GACNNNGTC	3 cut(s)	299, 709, 1307
VpaK11BI	GGWCC	4 cut(s)	493, 546, 1193, 1452
XapI	RAATTY	1 cut(s)	806
XceI	RCATGY	1 cut(s)	835
ZraI	GACGTC	1 cut(s)	586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.