MD07G1224000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009529
Physical Location & Seq
Forward (+)
30078115 .. 30080018
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1224000.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGCTGTTTCATTCCACTCGGTGCCGGGCTTCAATTCCACGGCTAAGTACCAGAATGTGTCAAAAGGTTCTGTTGGAGCTTCTATACCAGCGGCCACCACCCAATTCACATCCTTCTTTGGAGCGTTTGGACAAAGGAGGAATGTAAGCGAAAAACGATGGGGCTTATGTCTTTCAGTTGCAGATAGCAATCAGCTCACCACAGACACTGGTGTCATTGATAAATCGCCGCTAACAGCAAGGGAGAGATTGAGGGTGGCCAAGGTTCTCAGTCGTTACACAGACTCAAAGCCATTGAAAAAATCAGCTAGGGGCAAGAATGTATTAGATGCTCTCAGAGACAGCGAGCGTGGGAAGAAAAGGTCCGGACTTCCAGAGGCCCCTACAAACATTTTTGACGACAGCAAGCGAGGAATGCCTAAGCAGGGCCTAACTTGGGATTTTCCAGGGGGCAATGATCTGTTTCTAATTCTCTTTTCATTTGTATTTATCAGCACGGTGATGTTCGCTACAACGTTCGTAGTTTGGAAACTTGGTGCCATCCATTTCAATGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.48

Weight (kDa)

10.1

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccB1I GGYRCC 2 cut(s) 22, 536
AccIII TCCGGA 1 cut(s) 365
AciI CCGC 2 cut(s) 92, 230
AclI AACGTT 1 cut(s) 515
AcoI YGGCCR 2 cut(s) 93, 258
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 425, 436
AgsI TTSAA 3 cut(s) 34, 298, 550
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 3 cut(s) 80, 196, 308
AluI AGCT 3 cut(s) 80, 196, 308
Alw26I GTCTC 1 cut(s) 333
AlwNI CAGNNNCTG 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 365
AoxI GGCC 4 cut(s) 93, 258, 378, 427
AspS9I GGNCC 3 cut(s) 363, 379, 427
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 190, 511
AvaII GGWCC 1 cut(s) 363
BalI TGGCCA 1 cut(s) 260
BanI GGYRCC 2 cut(s) 22, 536
BccI CCATC 2 cut(s) 153, 548
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 447
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 1 cut(s) 309
BisI GCNGC 2 cut(s) 93, 230
BlsI GCNGC 2 cut(s) 94, 231
Bme1390I CCNGG 2 cut(s) 27, 447
Bme18I GGWCC 1 cut(s) 363
BmgT120I GGNCC 3 cut(s) 363, 379, 427
BmiI GGNNCC 3 cut(s) 24, 381, 538
BmrFI CCNGG 2 cut(s) 27, 447
BmsI GCATC 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 39, 261, 446
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 2 cut(s) 425, 436
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 460
Bse8I GATNNNNATC 1 cut(s) 189
BseAI TCCGGA 1 cut(s) 365
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 3 cut(s) 39, 261, 446
BseGI GGATG 2 cut(s) 110, 540
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 425, 436
BseMI GCAATG 1 cut(s) 460
BseMII CTCAG 2 cut(s) 283, 349
BseNI ACTGG 1 cut(s) 214
BshFI GGCC 4 cut(s) 95, 260, 380, 429
BshNI GGYRCC 2 cut(s) 22, 536
BsiSI CCGG 2 cut(s) 26, 366
BslI CCNNNNNNNGG 2 cut(s) 425, 436
BsmAI GTCTC 1 cut(s) 333
BsmI GAATGC 1 cut(s) 420
BsnI GGCC 4 cut(s) 95, 260, 380, 429
Bsp13I TCCGGA 1 cut(s) 365
Bsp143I GATC 1 cut(s) 457
BspACI CCGC 2 cut(s) 92, 230
BspANI GGCC 4 cut(s) 95, 260, 380, 429
BspCNI CTCAG 2 cut(s) 282, 348
BspEI TCCGGA 1 cut(s) 365
BspLI GGNNCC 3 cut(s) 24, 381, 538
BspT107I GGYRCC 2 cut(s) 22, 536
BsrDI GCAATG 1 cut(s) 460
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 3 cut(s) 39, 261, 446
BssMI GATC 1 cut(s) 457
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 1 cut(s) 499
BstC8I GCNNGC 2 cut(s) 347, 407
BstDEI CTNAG 4 cut(s) 45, 269, 335, 420
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 2 cut(s) 110, 540
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 1 cut(s) 457
BstMWI GCNNNNNNNGC 1 cut(s) 415
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 2 cut(s) 25, 445
BsuRI GGCC 4 cut(s) 95, 260, 380, 429
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 2 cut(s) 110, 540
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 347, 407
CaiI CAGNNNCTG 1 cut(s) 209
Cfr13I GGNCC 3 cut(s) 363, 379, 427
Csp6I GTAC 1 cut(s) 49
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 4 cut(s) 45, 269, 335, 420
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 22
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 2 cut(s) 93, 258
Eco130I CCWWGG 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 363
EcoO109I RGGNCCY 2 cut(s) 379, 427
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaiI YATR 2 cut(s) 86, 169
Fnu4HI GCNGC 2 cut(s) 93, 230
FokI GGATG 2 cut(s) 97, 527
Fsp4HI GCNGC 2 cut(s) 93, 230
FspBI CTAG 1 cut(s) 309
GluI GCNGC 2 cut(s) 93, 230
HaeIII GGCC 4 cut(s) 95, 260, 380, 429
HapII CCGG 2 cut(s) 26, 366
HinfI GANTC 1 cut(s) 284
HpaII CCGG 2 cut(s) 26, 366
HphI GGTGA 2 cut(s) 190, 511
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 2 cut(s) 366, 374
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 499
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 415
HpyF3I CTNAG 4 cut(s) 45, 269, 335, 420
HpySE526I ACGT 1 cut(s) 515
Kpn2I TCCGGA 1 cut(s) 365
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 2 cut(s) 77, 122
LpnPI CCDG 9 cut(s) 39, 65, 102, 195, 379, 387, 410, 432, 459
LweI GCATC 1 cut(s) 319
MaeI CTAG 1 cut(s) 309
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 1 cut(s) 275
MalI GATC 1 cut(s) 459
MboI GATC 1 cut(s) 457
MboII GAAGA 1 cut(s) 367
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 4 cut(s) 34, 104, 468, 556
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 4 cut(s) 132, 246, 370, 404
Mox20I TGGCCA 1 cut(s) 260
MroI TCCGGA 1 cut(s) 365
MscI TGGCCA 1 cut(s) 260
MslI CAYNNNNRTG 2 cut(s) 500, 549
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 26, 366
MspR9I CCNGG 2 cut(s) 27, 447
Mva1269I GAATGC 1 cut(s) 420
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 1 cut(s) 415
NciI CCSGG 1 cut(s) 27
NdeII GATC 1 cut(s) 457
NlaIV GGNNCC 3 cut(s) 24, 381, 538
PctI GAATGC 1 cut(s) 420
PkrI GCNGC 2 cut(s) 94, 231
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
Psp1406I AACGTT 1 cut(s) 515
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 3 cut(s) 24, 381, 538
PspPI GGNCC 3 cut(s) 363, 379, 427
PstNI CAGNNNCTG 1 cut(s) 209
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 2 cut(s) 500, 549
SatI GCNGC 2 cut(s) 93, 230
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 3 cut(s) 363, 379, 427
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 2 cut(s) 27, 447
SetI ASST 7 cut(s) 70, 82, 198, 267, 310, 365, 518
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 363
SmiMI CAYNNNNRTG 2 cut(s) 500, 549
Sse9I AATT 4 cut(s) 34, 104, 468, 556
SsiI CCGC 2 cut(s) 92, 230
SspMI CTAG 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 25, 445
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 499
TaiI ACGT 1 cut(s) 518
TasI AATT 4 cut(s) 34, 104, 468, 556
TauI GCSGC 2 cut(s) 95, 232
TscAI CASTG 1 cut(s) 214
TspDTI ATGAA 1 cut(s) 468
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 363
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.